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Gene information
General information
TC006533 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11111063..11113738
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006533
    iBeetle screen iB_04165

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva complete everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006533 RA
      MCCSNPAFY ISVYVLAVN LFGLYLRFV YFHNYFECF 
      ACRELDFKD QAYDYIVVG SGSAGSIVA RRLAENPSV 
      KVLLIEAGA SGNGILQIP TVSLMLQDS VFDWQYRTV 
      PQKHACLGL DKKVSHWPM GKILGGTAM LNNMIYVRG 
      HPQDFAEWY KDSCNFNYT IDVLPYFKK LESNETNKH 
      KCSVFVEDM PFKSNLSDY FLQAGLCLG FGLSDGVNS 
      EPGFSATKV TMRNGQRWT PYHQLEKTK KRNLVVITN 
      SLVEKVLLK SNYEAYGVK YTHLDETYY VRATKGVIL 
      SAGVIGSPK ILMLSGIGP KKHLEKLKI APRLDLPVG 
      ENLQDHVTT GLDLITLEA PPDMGLQQM LSPWSASRY 
      FLWGEGPWT SPGCESVGF FNSEDEKIP ELQFMILPY 
      GAAIDGGSY LRGLVGIGE RLWEGYFRR VNGSTMTVL 
      PVVLHPKSR GTVRLKSKD PRTPPLIDP NYLAEGYDV 
      DILLEGIEL VKEFLETPP MRRLGAKLN AVKFPGCEG 
      LEFDTRPYW VCYVRHFTL SSYHPVGTC ALGRVIDEG 
      FQVKGTNKL YVVDGSVLP SLPSGNPQG AIMMMAERA 
      AEIIKHHCW LSQRRCCSS DVFQDQCSC Y
    CDS sequence
    • >TC006533 RA
      atgtgttgc tccaaccct gcgttctac ataagtgtc 
      tacgttctc gctgttaat ctattcggg ctatacctt 
      agatttgtg tactttcac aactacttt gagtgcttc 
      gcttgtcga gagctggat ttcaaggac caggcctac 
      gattacatt gtagtcggg agcggctca gctggaagc 
      atcgtcgct cgaagactc gccgaaaac cccagcgtt 
      aaagttttg ctaattgaa gctggggct agcggaaat 
      ggcattttg caaatcccg actgttagt ctcatgcta 
      caagactcg gtttttgac tggcagtac cgaacggtg 
      ccccagaaa catgcctgt ttggggctt gacaaaaaa 
      gtaagtcat tggcccatg gggaagatt ctggggggc 
      acggcgatg ttaaataac atgatttac gtccgggga 
      cacccccaa gacttcgcc gagtggtat aaagattct 
      tgtaatttc aactacacg atcgacgtt cttccctac 
      ttcaaaaaa ctggaaagc aacgaaact aacaagcac 
      aaatgttca gttttcgtg gaggatatg ccgtttaag 
      tcgaacttg tcggattat tttttgcaa gccgggttg 
      tgtctcggc tttggccta tcagacggg gtgaactca 
      gagcctggt ttttcggcc actaaagta acaatgcgt 
      aacggccag cgctggacg ccttaccac caattagaa 
      aaaaccaaa aaacgcaat ctagtcgtc ataactaac 
      tctctagtc gaaaaagtc ctcttaaaa tccaactac 
      gaggcctac ggtgtgaaa tacacccac ttggacgaa 
      acctactac gtccgtgcc accaagggt gtgattcta 
      tcggccggc gtgatcggc tccccaaaa atcctcatg 
      ttatcgggc attggcccc aaaaaacac ttggagaaa 
      ttaaaaata gcccccaga cttgaccta ccagtcggc 
      gagaacttg caagaccac gtaaccacc ggactggac 
      ctcatcacg ctggaagcg ccccccgac atgggcctg 
      cagcaaatg ctgtcgccg tggagcgct agcaggtat 
      tttctgtgg ggggagggc ccctggacc agtccaggg 
      tgcgagagt gtcgggttt ttcaacagc gaggacgag 
      aaaatcccc gagttgcag ttcatgatt ctgccgtat 
      ggggccgcc attgacggg ggctcctac ttaaggggg 
      ctggtggga atcggggag agactctgg gaggggtat 
      tttcggcga gtgaacggc tcgactatg accgtgctc 
      ccggttgtt ttgcacccg aaaagtcgg gggaccgtg 
      aggctcaaa agtaaagac cccagaacg ccgcctttg 
      attgacccg aattatttg gcggagggg tacgatgtt 
      gatattctt ttggagggg attgagctg gttaaggag 
      tttttggaa acgccgcca atgcgccgc ctgggggct 
      aagctgaac gcggtcaag ttccccggt tgtgagggg 
      cttgagttt gacactaga ccctactgg gtgtgttat 
      gtccgccat tttacgtta tcaagttac caccctgtt 
      ggtacgtgc gctttgggg cgcgtgatt gacgagggg 
      tttcaagtc aaaggcacc aataaattg tacgttgtt 
      gacgggtcg gttttgccc agcttgccc agcggtaac 
      ccccagggg gctatcatg atgatggcg gagcgcgcg 
      gctgagata atcaagcac cactgctgg ttgagtcag 
      aggcggtgc tgctcgtcc gacgtgttc caagaccag 
      tgcagctgt tactaa
    mRNA sequence
    • >TC006533 RA
      tgcatcaaa tcaattagt acaaatcaa acattacgc 
      gtgtttttc agcaataaa taacccaga caaatttaa 
      ataaacttc ttgacaggt ggtttagtt gtaacctca 
      cttctattt ggcataacc tcacttttg acaattttc 
      ggccttctt tttttcaaa atttccgtg taaaattga 
      attaaacat aatgtgttg ctccaaccc tgcgttcta 
      cataagtgt ctacgttct cgctgttaa tctattcgg 
      gctatacct tagatttgt gtactttca caactactt 
      tgagtgctt cgcttgtcg agagctgga tttcaagga 
      ccaggccta cgattacat tgtagtcgg gagcggctc 
      agctggaag catcgtcgc tcgaagact cgccgaaaa 
      ccccagcgt taaagtttt gctaattga agctggggc 
      tagcggaaa tggcatttt gcaaatccc gactgttag 
      tctcatgct acaagactc ggtttttga ctggcagta 
      ccgaacggt gccccagaa acatgcctg tttggggct 
      tgacaaaaa agtaagtca ttggcccat ggggaagat 
      tctgggggg cacggcgat gttaaataa catgattta 
      cgtccgggg acaccccca agacttcgc cgagtggta 
      taaagattc ttgtaattt caactacac gatcgacgt 
      tcttcccta cttcaaaaa actggaaag caacgaaac 
      taacaagca caaatgttc agttttcgt ggaggatat 
      gccgtttaa gtcgaactt gtcggatta ttttttgca 
      agccgggtt gtgtctcgg ctttggcct atcagacgg 
      ggtgaactc agagcctgg tttttcggc cactaaagt 
      aacaatgcg taacggcca gcgctggac gccttacca 
      ccaattaga aaaaaccaa aaaacgcaa tctagtcgt 
      cataactaa ctctctagt cgaaaaagt cctcttaaa 
      atccaacta cgaggccta cggtgtgaa atacaccca 
      cttggacga aacctacta cgtccgtgc caccaaggg 
      tgtgattct atcggccgg cgtgatcgg ctccccaaa 
      aatcctcat gttatcggg cattggccc caaaaaaca 
      cttggagaa attaaaaat agcccccag acttgacct 
      accagtcgg cgagaactt gcaagacca cgtaaccac 
      cggactgga cctcatcac gctggaagc gccccccga 
      catgggcct gcagcaaat gctgtcgcc gtggagcgc 
      tagcaggta ttttctgtg gggggaggg cccctggac 
      cagtccagg gtgcgagag tgtcgggtt tttcaacag 
      cgaggacga gaaaatccc cgagttgca gttcatgat 
      tctgccgta tggggccgc cattgacgg gggctccta 
      cttaagggg gctggtggg aatcgggga gagactctg 
      ggaggggta ttttcggcg agtgaacgg ctcgactat 
      gaccgtgct cccggttgt tttgcaccc gaaaagtcg 
      ggggaccgt gaggctcaa aagtaaaga ccccagaac 
      gccgccttt gattgaccc gaattattt ggcggaggg 
      gtacgatgt tgatattct tttggaggg gattgagct 
      ggttaagga gtttttgga aacgccgcc aatgcgccg 
      cctgggggc taagctgaa cgcggtcaa gttccccgg 
      ttgtgaggg gcttgagtt tgacactag accctactg 
      ggtgtgtta tgtccgcca ttttacgtt atcaagtta 
      ccaccctgt tggtacgtg cgctttggg gcgcgtgat 
      tgacgaggg gtttcaagt caaaggcac caataaatt 
      gtacgttgt tgacgggtc ggttttgcc cagcttgcc 
      cagcggtaa cccccaggg ggctatcat gatgatggc 
      ggagcgcgc ggctgagat aatcaagca ccactgctg 
      gttgagtca gaggcggtg ctgctcgtc cgacgtgtt 
      ccaagacca gtgcagctg ttactaatt gtaataatc 
      ggtggtttt atttaaact acaccatac agttcactt 
      ctatttgaa tggtagagt acaatcata ttgctgcgt 
      ttgagcaaa ctgtgtaaa attacaaat attgcaaaa 
      gactagaat acagttttt acagtttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY