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Gene information
General information
TC006527 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11026880..11042792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006527
    iBeetle screen iB_04164

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006527 RA
      MVSFVLFAL VSAAWAEPL VRTCEPIRV DMCTNLGYN 
      MTEMPNLGG NDIQQEADY TLKSFSPLI QYGCSSQLK 
      LFLCSVYVP MCTEKVANP IGPCRGLCE SVRAKCYPV 
      LKGFGFSWP DALNCSRFP VENNHEHLC MEGPKDRGV 
      DVLAPVDPA VQKFDCGRN FVRNELGNC VPACDTNLM 
      FDDSEKNFA EVWVTVWAM ICFVISLAS ALTLTIGGG 
      RVKARPLIS IALCYCMVS AGWALRMFS GRMSPFCPP 
      EDGLSNVNC AFVFLLIYY FGMAANAWW VCLCAWWVA 
      RVGLAWPPE KLRSLGSVL HVCAWGLPA AQTVAALVR 
      RDVDPDELT GTCYIGNRN SATLLGLVL IPDFIYFML 
      GIFFLILGC VRVIRKPRP SAAAPLTAA TPRKESDFL 
      GAVCTLYAI PTFCVMASI YYEYNNRDQ WLNRESKPA 
      LWAFLLRHL MSLFIGVST IFWIWSMKT VTAWRAVLR 
      RLGPRKQLP VKVQTMPVL RYVPAHPVP STFSTSSRH 
      STRSHPHRK PRVHHIRTG GETVI
    CDS sequence
    • >TC006527 RA
      atggtttcg ttcgtgctg ttcgcgctc gtgtcggcg 
      gcctgggcc gagcccctg gtgcgcacc tgcgagccc 
      atccgcgtg gacatgtgc accaacctg ggctacaac 
      atgacggag atgcccaac ctcggcggc aacgacatc 
      cagcaggag gccgactac acgctcaag tccttctcg 
      ccgctgatc cagtacggc tgcagctcg cagctgaag 
      ctgttcctg tgctcggtg tacgtgccc atgtgcacg 
      gagaaggtg gccaacccc atcgggccc tgccgcggg 
      ctgtgcgag agcgtgcgg gccaagtgc taccccgtg 
      ctcaagggc ttcggcttc tcctggccg gacgccctc 
      aactgctcg cgcttcccg gtggagaac aaccacgag 
      cacctctgc atggagggc cccaaggac cggggggtg 
      gacgtgctg gcgcccgtg gaccccgcc gtgcagaag 
      ttcgactgc gggaggaat ttcgtcagg aatgagttg 
      gggaactgc gtgccggcc tgcgatacc aatctcatg 
      tttgacgat tcggagaag aactttgcc gaggtgtgg 
      gtgaccgtg tgggcaatg atttgcttc gtgatcagc 
      ttagcctcg gccctgacg ctgacaata ggcggaggc 
      cgcgtgaag gctcgccct ttgatcagc atagccctt 
      tgctactgc atggtgagc gctggctgg gccctccgc 
      atgttctcg ggccgcatg tcgcccttc tgcccgccc 
      gaggacggc ctttcgaac gtcaactgc gcgttcgtc 
      ttcctcctg atctactat ttcgggatg gcagcgaat 
      gcttggtgg gtctgttta tgcgcttgg tgggtcgca 
      cgtgtggga ctggcttgg cctccggaa aagctccga 
      agcctgggc tcggtgctg catgtgtgc gcttggggc 
      ttgccggca gcgcagact gtcgccgcc ttggtgcgg 
      cgggacgtg gacccggac gagctcact gggacctgt 
      tacatcggc aaccgcaac tcggccacc ctcctcggc 
      ctggtgctc atccccgac ttcatctac tttatgctg 
      ggaatattt ttcttaatc ctgggctgc gtgcgcgtg 
      atccgcaag ccgcgcccc tcggcggcg gcccccttg 
      acggcggcg acgccccgc aaagagagc gacttcctg 
      ggggccgtg tgcaccctc tacgccatc cccacgttc 
      tgcgtcatg gccagcatc tactacgag tacaacaac 
      cgcgaccag tggctgaac cgcgagtcc aaaccggcc 
      ttatgggcc ttcctcctg cgccacctc atgtcgctc 
      ttcatcggc gtcagcacc atcttctgg atctggtcg 
      atgaagact gtgacggcc tggcgggcg gttctgcgc 
      cggctgggc cccaggaag cagctgccg gtgaaggtg 
      cagacgatg ccggtgctg cggtacgtc ccggcgcac 
      ccagtgccc agcaccttc agcacgagc agcaggcac 
      tcgaccagg tcgcacccg caccggaag ccgagagtg 
      caccacatc cggaccggc ggggagact gttatttga
    mRNA sequence
    • >TC006527 RA
      acctagtga accaaacgc ggctccaat cggtcggag 
      agctcgcag tagagcggt cgtgccatg gtttcgttc 
      gtgctgttc gcgctcgtg tcggcggcc tgggccgag 
      cccctggtg cgcacctgc gagcccatc cgcgtggac 
      atgtgcacc aacctgggc tacaacatg acggagatg 
      cccaacctc ggcggcaac gacatccag caggaggcc 
      gactacacg ctcaagtcc ttctcgccg ctgatccag 
      tacggctgc agctcgcag ctgaagctg ttcctgtgc 
      tcggtgtac gtgcccatg tgcacggag aaggtggcc 
      aaccccatc gggccctgc cgcgggctg tgcgagagc 
      gtgcgggcc aagtgctac cccgtgctc aagggcttc 
      ggcttctcc tggccggac gccctcaac tgctcgcgc 
      ttcccggtg gagaacaac cacgagcac ctctgcatg 
      gagggcccc aaggaccgg ggggtggac gtgctggcg 
      cccgtggac cccgccgtg cagaagttc gactgcggg 
      aggaatttc gtcaggaat gagttgggg aactgcgtg 
      ccggcctgc gataccaat ctcatgttt gacgattcg 
      gagaagaac tttgccgag gtgtgggtg accgtgtgg 
      gcaatgatt tgcttcgtg atcagctta gcctcggcc 
      ctgacgctg acaataggc ggaggccgc gtgaaggct 
      cgccctttg atcagcata gccctttgc tactgcatg 
      gtgagcgct ggctgggcc ctccgcatg ttctcgggc 
      cgcatgtcg cccttctgc ccgcccgag gacggcctt 
      tcgaacgtc aactgcgcg ttcgtcttc ctcctgatc 
      tactatttc gggatggca gcgaatgct tggtgggtc 
      tgtttatgc gcttggtgg gtcgcacgt gtgggactg 
      gcttggcct ccggaaaag ctccgaagc ctgggctcg 
      gtgctgcat gtgtgcgct tggggcttg ccggcagcg 
      cagactgtc gccgccttg gtgcggcgg gacgtggac 
      ccggacgag ctcactggg acctgttac atcggcaac 
      cgcaactcg gccaccctc ctcggcctg gtgctcatc 
      cccgacttc atctacttt atgctggga atatttttc 
      ttaatcctg ggctgcgtg cgcgtgatc cgcaagccg 
      cgcccctcg gcggcggcc cccttgacg gcggcgacg 
      ccccgcaaa gagagcgac ttcctgggg gccgtgtgc 
      accctctac gccatcccc acgttctgc gtcatggcc 
      agcatctac tacgagtac aacaaccgc gaccagtgg 
      ctgaaccgc gagtccaaa ccggcctta tgggccttc 
      ctcctgcgc cacctcatg tcgctcttc atcggcgtc 
      agcaccatc ttctggatc tggtcgatg aagactgtg 
      acggcctgg cgggcggtt ctgcgccgg ctgggcccc 
      aggaagcag ctgccggtg aaggtgcag acgatgccg 
      gtgctgcgg tacgtcccg gcgcaccca gtgcccagc 
      accttcagc acgagcagc aggcactcg accaggtcg 
      cacccgcac cggaagccg agagtgcac cacatccgg 
      accggcggg gagactgtt atttgagga aattcgggg 
      ggggggcga agaaaagat ctctgtgtt ttggggact 
      ttgggggtt aagccagtt agctctttt caagtccgg 
      atttgtaaa tattctgtg atcggtgcg ttgcgacat 
      ttgtttatt ctgggattt gtgttttgc cataacagc 
      tcgttctgt gtgcatgaa aacaaacaa ccgatttat 
      cgacagatg gttcggttt ggggcttgc ggactcttg 
      cgcgcgtaa gagaccgac aagattgtt tctcttcaa 
      gacatagga gagggtcct tatagactt cgattcatt 
      ttttcatta ttttctcat ttttttttt ccatgaatt 
      aacgttata ttccttgta aatgtgcct tgaacggat 
      gtacataaa agtgccatt gtgtagtat actatctaa 
      agcggggga taaatcgac gaaccgtct cttgccaaa 
      ttaccttca tggcgagtt gtcgattaa tccggcgct 
      tttctcaga atttttatt tttttcgta cgatttttt 
      atacaaatc tttataatg tatttgtaa ttatattta 
      tttagttat tatgagtgt aaaattgtt ttaaatatt 
      gtgatacgt tttttgaat atttgtaga tagacaata 
      aaacacaaa aaacatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY