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Gene information
General information
TC006523 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10974588..10978426
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006523
    iBeetle screen iB_04163

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006523 RA
      MAVTIDSRE LFNSLSTKI TQLSYGRAK NDRSVINST 
      LDELNSFNY KYPIVANPT KAVLLVNQC CSLIPPEDD 
      ELVLKCCQL ITNLITRQK VVIDGRTLT VTVLWCLQA 
      LKDQDSSTD LLVALDALL RANAGHELI TEIVKNSTL 
      LNLAKNEQK PELAFFALQ CLEACTIPA DDTKTLPPD 
      ELKNEFQTS FEIFLKHTV KNHFKLDKL ISAKIYIVC 
      LRGLQNVIK QKPDLLSDN LGIILGVVK SYMLLGISG 
      IEFVKPQKL MPSPLSVPE TSTTESRGK GGKMTKQRK 
      PKTQPTIMK TRKSEKWDD DTGGYTPAV TMNTDESVP 
      NFGNSVKTS DSDFSDTEG GNLAKLNVM NGRVRQAAL 
      GLFLHTIKL THKTVIFSY WSSFIPENP LSTKHNLMT 
      CILKETSNR GSMAALNAL LVLLSLSKV YLAQAEKGE 
      NLAFTPFSV VLGLTIKEL HKCLSLALS AHSIPVLTM 
      VLKCFAALV QATPYHRLA PGLITKLIR NVKPLVSHK 
      DSTVQVAAL IVLGCVLAS EPIIPESRE AFLKTNEPE 
      TPWLLHRCL ANLEQDNVP TPVKLESLQ IISVMSRNY 
      FEDLVAPYL DHVTKALDL SLASKYTDV QLHAGRAVD 
      FIGQAMSRC AAPCLHFWH SLLQGSLTS LLQTEQPVL 
      RAIGCDCLG SIGAEAFEQ LPRDKQILC VTLLFTSSR 
      DEENAVKGA AVRALAICV LYPSLREDS GFVVDTAER 
      IYSVLGDEN FAVRVKCSW ALGNLSDAL VLNKGSALL 
      VDEFPKSLI LKLLKGTIK ASSDGDKIK MNTVRALGN 
      LLQLIDESL IGEREFRDA VQEGIGILV KNCTSGSNM 
      KVRWNSCYA IGNAMKNSF LFSLQNNWQ ERVFNSLSD 
      LVVNFRNFK VRINAALAL SSPQKREFY GRFYQPVWV 
      SLIKGLETS ENVADFNEY KHRDNLVEQ ICLTLGHLM 
      TLLTKEDLA FIKDGVNSD YLNIQMKRV WEKLLPEKS 
      TVLFDAQNY VTSLKNNPD LSDGQKNTL DCLHNILKQ 
      DL
    CDS sequence
    • >TC006523 RA
      atggccgtc accattgac agtcgagaa cttttcaac 
      agtctgtca acaaaaatc acacaatta tcgtacgga 
      cgcgctaaa aacgaccgc tctgtgata aattcaacc 
      cttgacgag ctaaattct ttcaattat aagtaccca 
      atcgttgca aacccgact aaagctgtc cttttggta 
      aaccaatgt tgctccttg ataccgccc gaagacgac 
      gaactcgtg ttgaaatgt tgccagtta attaccaat 
      ttaattacg cgccagaag gtggtcata gacggacgc 
      acgctcact gtgaccgtt ttgtggtgc ctccaagct 
      ttaaaagac caagactcc agtacggac ctccttgtt 
      gccttagac gccctactg cgtgcaaat gctggacat 
      gaattaatt accgaaatt gtgaaaaac agtacgctc 
      ctgaattta gcaaaaaat gagcagaag ccggaattg 
      gcattcttt gcccttcag tgtcttgaa gcgtgcacg 
      atacctgcc gatgatact aaaacctta cctccagac 
      gagttgaaa aatgagttt caaaccagt tttgaaatt 
      tttttgaaa cacacagtt aaaaaccat tttaaattg 
      gataaattg ataagtgcg aaaatctac attgtttgc 
      ttgaggggg ttgcaaaat gtaatcaag caaaaacct 
      gatttgctc tccgataat ttggggata atactaggg 
      gttgtaaag tcctacatg ttgctcgga atttctgga 
      attgagttt gtgaagccg cagaaattg atgccttcg 
      cctttgagt gtccctgag actagcacg actgagtct 
      agggggaaa ggaggaaag atgactaag caaagaaag 
      cccaaaact cagcccaca attatgaaa actcgaaaa 
      tcggaaaaa tgggacgat gacactggt ggttacact 
      cccgctgta acaatgaac accgacgaa tcagtgcct 
      aattttggc aatagtgtc aaaacaagc gactctgat 
      ttttccgac acagagggc ggaaatcta gcgaaacta 
      aacgtaatg aatggaagg gtgcgtcag gcagccctt 
      ggcttattt ttacacaca attaagcta acacacaag 
      actgtgatt ttttcgtac tggtcgagt ttcatcccc 
      gaaaacccg ctttccacc aaacacaac ttaatgact 
      tgcatactt aaggaaacg tccaatcgg gggagcatg 
      gcggctttg aacgccctc ctagtccta ctttcgctg 
      tccaaagtg tatctagca caggcggaa aaaggcgaa 
      aatctcgcc tttacaccg ttctccgtc gtgttgggt 
      ctaacaatc aaagaattg cacaaatgt ctaagtcta 
      gcattaagt gcgcactcg atccctgtg ctaacaatg 
      gttttgaag tgtttcgcg gctttggtc caagccacg 
      ccttatcac cggctagcc cccggcctt atcaccaaa 
      ctgataagg aacgtcaaa cctttggtc agccacaaa 
      gactcaacg gttcaagtt gccgctttg atcgtcctg 
      gggtgtgtg ttagcctcg gaaccgatc attcccgag 
      tcacgggaa gcgttcctc aaaacgaac gaacccgaa 
      actccatgg ctgctccat cggtgtctc gccaacttg 
      gagcaggac aacgtcccc actccagtc aaactcgaa 
      tcgctccaa ataatctca gtcatgagc cgaaattat 
      ttcgaggat ttagtcgcc ccctatttg gaccacgtg 
      actaaagcg ctcgattta tcactggct agtaaatac 
      accgacgtg cagttgcat gcaggacgt gctgttgac 
      ttcataggc caggcaatg agtcgttgt gcagcccca 
      tgtctccac ttctggcac tcgctcctg caagggtca 
      ctcacgtcg ctcttgcaa accgagcag ccggtttta 
      agggccata ggctgcgat tgtctgggg agtatcggg 
      gcggaggcg tttgagcaa ctcccccgt gacaagcaa 
      attctatgt gtcaccctc ctctttacc agcagtcgc 
      gacgaggaa aacgccgtt aagggggcg gcggttagg 
      gctttggcc atttgtgtc ctttatccg agcttgaga 
      gaggattca ggctttgtg gtggatacc gcagaacgg 
      atatacagt gtgttgggg gatgagaat ttcgcggtt 
      cgggttaag tgttcgtgg gctttgggg aatctgagc 
      gatgcgctt gttttaaac aagggttca gcgcttctc 
      gtggacgag tttcccaaa agtttgatt ttgaagttg 
      ttgaaaggc acgattaaa gcgtcaagt gacggcgat 
      aaaattaaa atgaacacg gtgcgggcg ttggggaat 
      cttttacaa ttgattgac gaaagtttg attggggaa 
      cgagagttt cgggatgcg gtgcaagag gggattggt 
      attttggtg aaaaactgc acctcgggg tcaaatatg 
      aaggtgcga tggaattcg tgttacgcc atcgggaac 
      gccatgaaa aattcattt cttttctcg ttacaaaac 
      aactggcag gagagggtt tttaactcg ttgtccgac 
      ttagtggtg aatttccgt aactttaaa gtgcggatt 
      aatgcagca cttgcgctc tcatcgcct cagaagaga 
      gagttttac ggacggttt taccaacca gtttgggtt 
      tcactcatt aaagggcta gaaacgtcc gaaaacgtt 
      gccgatttt aacgaatat aaacaccga gataatttg 
      gtggaacaa atttgttta acgcttggt catttgatg 
      acgctactc acaaaagaa gatttggca tttattaaa 
      gatggtgtt aattctgac tatttaaac attcagatg 
      aagagggtt tgggaaaaa ttgctaccc gaaaaatcg 
      actgtgcta tttgatgca caaaactat gttaccagc 
      ttaaaaaac aatcccgac ctcagcgat gggcaaaaa 
      aatacactc gactgtcta cacaatatt ttgaaacaa 
      gatttataa
    mRNA sequence
    • >TC006523 RA
      ttgttcccc aatgattaa ataaaaatt aatttcgca 
      caaagactc aaactgcaa tgttggcgc cttgacgaa 
      acgaccaaa catggccgt caccattga cagtcgaga 
      acttttcaa cagtctgtc aacaaaaat cacacaatt 
      atcgtacgg acgcgctaa aaacgaccg ctctgtgat 
      aaattcaac ccttgacga gctaaattc tttcaatta 
      taagtaccc aatcgttgc aaacccgac taaagctgt 
      ccttttggt aaaccaatg ttgctcctt gataccgcc 
      cgaagacga cgaactcgt gttgaaatg ttgccagtt 
      aattaccaa tttaattac gcgccagaa ggtggtcat 
      agacggacg cacgctcac tgtgaccgt tttgtggtg 
      cctccaagc tttaaaaga ccaagactc cagtacgga 
      cctccttgt tgccttaga cgccctact gcgtgcaaa 
      tgctggaca tgaattaat taccgaaat tgtgaaaaa 
      cagtacgct cctgaattt agcaaaaaa tgagcagaa 
      gccggaatt ggcattctt tgcccttca gtgtcttga 
      agcgtgcac gatacctgc cgatgatac taaaacctt 
      acctccaga cgagttgaa aaatgagtt tcaaaccag 
      ttttgaaat ttttttgaa acacacagt taaaaacca 
      ttttaaatt ggataaatt gataagtgc gaaaatcta 
      cattgtttg cttgagggg gttgcaaaa tgtaatcaa 
      gcaaaaacc tgatttgct ctccgataa tttggggat 
      aatactagg ggttgtaaa gtcctacat gttgctcgg 
      aatttctgg aattgagtt tgtgaagcc gcagaaatt 
      gatgccttc gcctttgag tgtccctga gactagcac 
      gactgagtc tagggggaa aggaggaaa gatgactaa 
      gcaaagaaa gcccaaaac tcagcccac aattatgaa 
      aactcgaaa atcggaaaa atgggacga tgacactgg 
      tggttacac tcccgctgt aacaatgaa caccgacga 
      atcagtgcc taattttgg caatagtgt caaaacaag 
      cgactctga tttttccga cacagaggg cggaaatct 
      agcgaaact aaacgtaat gaatggaag ggtgcgtca 
      ggcagccct tggcttatt tttacacac aattaagct 
      aacacacaa gactgtgat tttttcgta ctggtcgag 
      tttcatccc cgaaaaccc gctttccac caaacacaa 
      cttaatgac ttgcatact taaggaaac gtccaatcg 
      ggggagcat ggcggcttt gaacgccct cctagtcct 
      actttcgct gtccaaagt gtatctagc acaggcgga 
      aaaaggcga aaatctcgc ctttacacc gttctccgt 
      cgtgttggg tctaacaat caaagaatt gcacaaatg 
      tctaagtct agcattaag tgcgcactc gatccctgt 
      gctaacaat ggttttgaa gtgtttcgc ggctttggt 
      ccaagccac gccttatca ccggctagc ccccggcct 
      tatcaccaa actgataag gaacgtcaa acctttggt 
      cagccacaa agactcaac ggttcaagt tgccgcttt 
      gatcgtcct ggggtgtgt gttagcctc ggaaccgat 
      cattcccga gtcacggga agcgttcct caaaacgaa 
      cgaacccga aactccatg gctgctcca tcggtgtct 
      cgccaactt ggagcagga caacgtccc cactccagt 
      caaactcga atcgctcca aataatctc agtcatgag 
      ccgaaatta tttcgagga tttagtcgc cccctattt 
      ggaccacgt gactaaagc gctcgattt atcactggc 
      tagtaaata caccgacgt gcagttgca tgcaggacg 
      tgctgttga cttcatagg ccaggcaat gagtcgttg 
      tgcagcccc atgtctcca cttctggca ctcgctcct 
      gcaagggtc actcacgtc gctcttgca aaccgagca 
      gccggtttt aagggccat aggctgcga ttgtctggg 
      gagtatcgg ggcggaggc gtttgagca actcccccg 
      tgacaagca aattctatg tgtcaccct cctctttac 
      cagcagtcg cgacgagga aaacgccgt taagggggc 
      ggcggttag ggctttggc catttgtgt cctttatcc 
      gagcttgag agaggattc aggctttgt ggtggatac 
      cgcagaacg gatatacag tgtgttggg ggatgagaa 
      tttcgcggt tcgggttaa gtgttcgtg ggctttggg 
      gaatctgag cgatgcgct tgttttaaa caagggttc 
      agcgcttct cgtggacga gtttcccaa aagtttgat 
      tttgaagtt gttgaaagg cacgattaa agcgtcaag 
      tgacggcga taaaattaa aatgaacac ggtgcgggc 
      gttggggaa tcttttaca attgattga cgaaagttt 
      gattgggga acgagagtt tcgggatgc ggtgcaaga 
      ggggattgg tattttggt gaaaaactg cacctcggg 
      gtcaaatat gaaggtgcg atggaattc gtgttacgc 
      catcgggaa cgccatgaa aaattcatt tcttttctc 
      gttacaaaa caactggca ggagagggt ttttaactc 
      gttgtccga cttagtggt gaatttccg taactttaa 
      agtgcggat taatgcagc acttgcgct ctcatcgcc 
      tcagaagag agagtttta cggacggtt ttaccaacc 
      agtttgggt ttcactcat taaagggct agaaacgtc 
      cgaaaacgt tgccgattt taacgaata taaacaccg 
      agataattt ggtggaaca aatttgttt aacgcttgg 
      tcatttgat gacgctact cacaaaaga agatttggc 
      atttattaa agatggtgt taattctga ctatttaaa 
      cattcagat gaagagggt ttgggaaaa attgctacc 
      cgaaaaatc gactgtgct atttgatgc acaaaacta 
      tgttaccag cttaaaaaa caatcccga cctcagcga 
      tgggcaaaa aaatacact cgactgtct acacaatat 
      tttgaaaca agatttata aattattta ttttttata 
      gaaatatac gattttgtt tatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY