Status
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Gene information
General information
TC006514 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10846195..10848469
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006514
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04162

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006514 RA
      MTTTKSNMR ELEAAAKDS ARAHVVNMF QRPGQLEKV 
      DHLKRRLTR KKNSVEALL KSAMQQQLD GVRVGLNQL 
      HCCLEDVRE IDSSIKKMF TLFNEVPDL CAKLSQVRD 
      ENMRHSQYV TARENLKHI FTVPESVEK TKQWINEGK 
      LLHTHQCLR DLEMSRDDL LYELHKLPN QSPHDKSML 
      KAYFADVET LSNLLEKQL RLILSRTLN TVRKEPTVI 
      VTALRIIER EEKTDQEAL KQEKQTGFL PPGRPKKWK 
      QMAFEVLEK SVATRIEGT QVDERDDNK HWLIRYLEL 
      TRQLILEDL RVVKTLCVP CFPPRYGII DKYIQMYHH 
      CLSRHLQEV IQNGIEGNE YVTILSWVS KTYQGVELL 
      GHPDLVEYT KHLEPLLPQ PVMETLEAE YLRNIETNY 
      GEWMRNTLN SEEKEWRSN IEPNSENYT RTAAPIIIF 
      QMIDQNLQV CKTISDEMT CKVLILSIE QVINFGDMY 
      RRAIVEFKN KYFEDRKQV PYFTQYMIM IVNNCLQLA 
      DLGSQLEKQ YWAPTSPPT LGDHFNRLR TTFIQLRND 
      SGDFLLEEL FLDLDKHFD ELFTPRWLG TSHPVDTIC 
      ATLDDYFQD YNRLSEPNY KFIVHQARA LVSKRYLTA 
      LLSKKGTFK TYEESVQAS GKIVKEANQ LGKLLDTLC 
      SLEGDDPFE SVILLTEVL KSDDDMLSF ELHRIVEKY 
      PDITEDHLL RLLSLRGDL ARSDIRDKV AHLPKVKAT 
      HQSSVFRTL VFPKLVNLN VFNN
    CDS sequence
    • >TC006514 RA
      atgaccaca acaaagtca aatatgcgc gaattggag 
      gcggcggcc aaagactcg gcccgggcg cacgtggtg 
      aacatgttc caacgtcca ggccagttg gaaaaagtc 
      gaccacttg aagcgccgc ctcacccgc aagaaaaac 
      tccgtggag gcgctcctc aagtccgcg atgcagcaa 
      caactcgac ggagtccgc gtcggactc aaccaactg 
      cactgttgt ctcgaagac gtccgcgag atcgactca 
      agcatcaaa aaaatgttc actctcttc aacgaagtc 
      cccgacttg tgcgccaag ctgagccaa gtccgggac 
      gaaaacatg cggcattcg cagtacgtg accgcgcgc 
      gagaacttg aagcacatc ttcacggtc cccgaaagt 
      gtggagaag accaaacag tggattaac gaagggaaa 
      ttgctccac acccatcag tgtctgagg gatttggaa 
      atgtcccgc gatgattta ctgtacgag ttgcataag 
      ttgccaaac cagtcaccg catgataaa tccatgctt 
      aaagcgtac tttgccgac gttgagact ttgtccaat 
      ttattggag aaacagttg aggctgatt ttgtcgcgt 
      acgctcaac acggtgcgg aaggaacct actgtgatt 
      gtcacagct ctccggata attgaacgg gaggagaaa 
      accgaccaa gaggcactt aaacaggaa aaacagacc 
      gggttcttg cccccaggg cggcccaaa aaatggaaa 
      caaatggcg ttcgaagtg ttggaaaag tcagtcgct 
      actagaatt gaaggcacg caagtcgac gaacgcgat 
      gacaacaaa cactggctc attcgttac ttggaattg 
      acgcggcag ctcattttg gaggacttg cgagttgtt 
      aaaacactg tgtgtcccg tgtttcccg cccaggtac 
      gggatcatc gacaagtac atacagatg taccaccac 
      tgcctgtcc cgccactta caagaagtg attcagaac 
      ggaatcgaa ggcaacgaa tacgtgacc atcctctca 
      tgggtcagt aaaacctac caaggggtt gagctactg 
      ggccacccg gacttggtc gaatacacc aaacacctg 
      gagcctttg ctcccccag cccgtgatg gagaccctc 
      gaggccgag tatttgcgc aacattgag acaaactac 
      ggggaatgg atgcgaaac accctaaac tcagaggag 
      aaagagtgg cgctcgaac atcgaaccc aactcggag 
      aactacacc cgcacggca gcccccatt atcatcttc 
      caaatgatc gaccagaac ttacaagtc tgcaagacg 
      ataagcgac gaaatgacg tgcaaagtc ctcatcttg 
      agcatagag caagtcatc aatttcggg gacatgtac 
      cggagagca atcgtcgag ttcaaaaac aaatacttc 
      gaggaccgc aagcaagtc ccgtacttc acccagtac 
      atgatcatg atcgtcaac aactgcctc caactggcc 
      gatttgggg tcacagttg gagaagcag tactgggcc 
      cccacgtcg ccgccaact ctcggcgat catttcaac 
      cgcttgcgg acgactttc atccagttg cgcaacgac 
      tccggcgat ttcttactc gaggagttg ttcctggac 
      ttggacaaa cactttgac gaattgttc accccgcgc 
      tggctgggg acttcgcac cccgtcgac acgatttgt 
      gcgactctc gacgactac ttccaagac tacaatcgc 
      ctcagcgaa cccaactac aagtttatt gtgcaccag 
      gcccgcgct ttggtctcc aaacgctac ttaaccgcg 
      ctattgtcc aaaaaaggc acttttaag acgtatgaa 
      gagagcgtg caagccagc ggaaagatc gtcaaggag 
      gccaatcag ttgggaaag ttactagac acactgtgc 
      agtttagaa ggcgatgac ccgtttgag agcgttatt 
      ttgttaacg gaagtattg aagagcgac gatgatatg 
      ctatccttt gagctacat cggattgtt gaaaagtat 
      cccgatatc accgaagat catttattg cgactgttg 
      agtctgagg ggtgacctt gccaggtcg gacatccgc 
      gataaagtc gcgcatttg cccaaagtt aaggccacg 
      caccagagc agcgtgttc cggacgctg gtgttcccc 
      aaacttgtt aatctcaac gtattcaac aattaa
    mRNA sequence
    • >TC006514 RA
      taggttagg tcaaaacac ccccaaaaa tgaccacaa 
      caaagtcaa atatgcgcg aattggagg cggcggcca 
      aagactcgg cccgggcgc acgtggtga acatgttcc 
      aacgtccag gccagttgg aaaaagtcg accacttga 
      agcgccgcc tcacccgca agaaaaact ccgtggagg 
      cgctcctca agtccgcga tgcagcaac aactcgacg 
      gagtccgcg tcggactca accaactgc actgttgtc 
      tcgaagacg tccgcgaga tcgactcaa gcatcaaaa 
      aaatgttca ctctcttca acgaagtcc ccgacttgt 
      gcgccaagc tgagccaag tccgggacg aaaacatgc 
      ggcattcgc agtacgtga ccgcgcgcg agaacttga 
      agcacatct tcacggtcc ccgaaagtg tggagaaga 
      ccaaacagt ggattaacg aagggaaat tgctccaca 
      cccatcagt gtctgaggg atttggaaa tgtcccgcg 
      atgatttac tgtacgagt tgcataagt tgccaaacc 
      agtcaccgc atgataaat ccatgctta aagcgtact 
      ttgccgacg ttgagactt tgtccaatt tattggaga 
      aacagttga ggctgattt tgtcgcgta cgctcaaca 
      cggtgcgga aggaaccta ctgtgattg tcacagctc 
      tccggataa ttgaacggg aggagaaaa ccgaccaag 
      aggcactta aacaggaaa aacagaccg ggttcttgc 
      ccccagggc ggcccaaaa aatggaaac aaatggcgt 
      tcgaagtgt tggaaaagt cagtcgcta ctagaattg 
      aaggcacgc aagtcgacg aacgcgatg acaacaaac 
      actggctca ttcgttact tggaattga cgcggcagc 
      tcattttgg aggacttgc gagttgtta aaacactgt 
      gtgtcccgt gtttcccgc ccaggtacg ggatcatcg 
      acaagtaca tacagatgt accaccact gcctgtccc 
      gccacttac aagaagtga ttcagaacg gaatcgaag 
      gcaacgaat acgtgacca tcctctcat gggtcagta 
      aaacctacc aaggggttg agctactgg gccacccgg 
      acttggtcg aatacacca aacacctgg agcctttgc 
      tcccccagc ccgtgatgg agaccctcg aggccgagt 
      atttgcgca acattgaga caaactacg gggaatgga 
      tgcgaaaca ccctaaact cagaggaga aagagtggc 
      gctcgaaca tcgaaccca actcggaga actacaccc 
      gcacggcag cccccatta tcatcttcc aaatgatcg 
      accagaact tacaagtct gcaagacga taagcgacg 
      aaatgacgt gcaaagtcc tcatcttga gcatagagc 
      aagtcatca atttcgggg acatgtacc ggagagcaa 
      tcgtcgagt tcaaaaaca aatacttcg aggaccgca 
      agcaagtcc cgtacttca cccagtaca tgatcatga 
      tcgtcaaca actgcctcc aactggccg atttggggt 
      cacagttgg agaagcagt actgggccc ccacgtcgc 
      cgccaactc tcggcgatc atttcaacc gcttgcgga 
      cgactttca tccagttgc gcaacgact ccggcgatt 
      tcttactcg aggagttgt tcctggact tggacaaac 
      actttgacg aattgttca ccccgcgct ggctgggga 
      cttcgcacc ccgtcgaca cgatttgtg cgactctcg 
      acgactact tccaagact acaatcgcc tcagcgaac 
      ccaactaca agtttattg tgcaccagg cccgcgctt 
      tggtctcca aacgctact taaccgcgc tattgtcca 
      aaaaaggca cttttaaga cgtatgaag agagcgtgc 
      aagccagcg gaaagatcg tcaaggagg ccaatcagt 
      tgggaaagt tactagaca cactgtgca gtttagaag 
      gcgatgacc cgtttgaga gcgttattt tgttaacgg 
      aagtattga agagcgacg atgatatgc tatcctttg 
      agctacatc ggattgttg aaaagtatc ccgatatca 
      ccgaagatc atttattgc gactgttga gtctgaggg 
      gtgaccttg ccaggtcgg acatccgcg ataaagtcg 
      cgcatttgc ccaaagtta aggccacgc accagagca 
      gcgtgttcc ggacgctgg tgttcccca aacttgtta 
      atctcaacg tattcaaca attaaatct ataagatag 
      aataggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY