Status
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Gene information
General information
TC034109
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006511,TC006510,TC006509,TC006513,TC006512
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10778435..10839061
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034109
    iBeetle screen iB_04161

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 5
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    tropharium anterior irregular - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber partially not present - Number of animals showing the phenotype: 4
    Comment: no late vitellogenic egg chambers
    egg chamber partially degenerated - Number of animals showing the phenotype: 4
    egg chamber stage sequence irregular - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034109 RA
      MRVCFGTLC FSSRLTAND EEAAPGLVG AATVTTTTA 
      LNGHHRQAM ASSVDELQA LLAEKETKI QELTKLVQQ 
      KDLEITNLR SQLDKFQSV LPLYNPTSP KHYVGLNNN 
      AVLRPRKQR AGISAEPQS EASILELSK ETFPTYNKD 
      ESSRELIKA AILDNDFMK NLESTQIKE IVDCMYPEA 
      YTAGSLIIK EGDVGSIVY VLEAVSLLS CNFLLPLSV 
      NVHISKATV SRSAPSPST SCNENVKCS SASEDISIT 
      KCKIWWSCC QARKNPEVT HFRPFFKPS ESIGATGQN 
      TMKIKHYPG KAVINDPAW LEMDISNDI RGRQYEANW 
      EDTQRNLSS TNSSGSNSW DKYSEGTIS SKLGHYQKV 
      LDDLAQEEL YTQPIKKEE KPKYHSEKC LARINNNTV 
      PNYYLPSQA KIQKSRSFK ETEDKPKPK PRTKLAQNL 
      KNVVNDLEK QIKSVRFTE EEEAEPGQR ISQWIDDQN 
      KYILTDSVL IKGEDLPKK PRQRIPPRN LTPSPSEVS 
      DERSSAYNL YKSGAKAQV LEAQNSDFL IPRPKLIVP 
      VHTYAVRRR RTGNLQCAG EEVIKNKDS EGCVEVSRE 
      NKFLSTLTP GKVLGELAI LYNCQRTAT IKAATDCKL 
      WAIERQCFQ TIMMRTGLI RQAEYTDFL KSVPIFKNL 
      PEDTLIKIS DVLEETFYA NGDYIIRQG ARGDTFFII 
      SKGTVKVTK KVPDSNEEK YIRTLGKGD FFGEKALQG 
      DDLRTANII VDNPEGVYC LVIDRETFN QLISNLDEI 
      RTKYKDEGV DRRRENEEF EHVQLTDLK KLTTLGVGG 
      FGRVELVQI QGDSNRSFA LKQMKKAQI VETRQQQHI 
      MSEKEIMGE ANCDFIVKL FKTFKDRKY LYMLMESCL 
      GGELWTVLR DKGHFDDAT TRFYTACVV EAFDYLHSR 
      NIIYRDLKP ENLLLDNQG YVKLVDFGF AKKLHSGRK 
      TWTFCGTPE YVAPEVILN KGHDISADY WSLGVLMFE 
      LLTGTPPFT GADPMKTYN IILKGIDAI DFPRNITRN 
      AMALIKKLC RDNPAERLG YQKGGISEI QKHKWFDGF 
      NWEGLVNRT LTPPILPQV KHVTDTSNF DDYPPDTDT 
      PPPDDVSGW DADF
    • >TC034109 RB
      MSCYNFISK VLTRRKYHS EMPPSEAEV VQSLKKMLE 
      DKTRELSLK EETIALLEK ELDDKDAII KHLHNEIDK 
      FRQVVKPIT QKIITKQLT LGDGKPRTK RQAISAEPL 
      NDEFGDLQI KKIPKSATS RELIKAAIL DNDFMKNLE 
      STQIKEIVD CMYPEAYTA GSLIIKEGD VGSIVYVLE 
      AVSLLSCNF LLPLSVNVH ISKATVSRS APSPSTSCN 
      ENVKCSSAS EDISITKCK IWWSCCQAR KNPEVTHFR 
      PFFKPSESI GATGQNTMK IKHYPGKAV INDPAWLEM 
      DISNDIRGR QYEANWEDT QRNLSSTNS SGSNSWDKY 
      SEGTISSKL GHYQKVLDD LAQEELYTQ PIKKEEKPK 
      YHSEKCLAR INNNTVPNY YLPSQAKIQ KSRSFKETE 
      DKPKPKPRT KLAQNLKNV VNDLEKQIK SVRFTEEEE 
      AEPGQRISQ WIDDQNKYI LTDSVLIKG EDLPKKPRQ 
      RIPPRNLTP SPSEVSDER SSAYNLYKS GAKAQVLEA 
      QNSDFLIPR PKLIVPVHT YAVRRRRTG NLQCAGEEV 
      IKNKDSEGC VEVSRENKF LSTLTPGKV LGELAILYN 
      CQRTATIKA ATDCKLWAI ERQCFQTIM MRTGLIRQA 
      EYTDFLKSV PIFKNLPED TLIKISDVL EETFYANGD 
      YIIRQGARG DTFFIISKG TVKVTKKVP DSNEEKYIR 
      TLGKGDFFG EKALQGDDL RTANIIVDN PEGVYCLVI 
      DRETFNQLI SNLDEIRTK YKDEGVDRR RENEEFEHV 
      QLTDLKKLT TLGVGGFGR VELVQIQGD SNRSFALKQ 
      MKKAQIVET RQQQHIMSE KEIMGEANC DFIVKLFKT 
      FKDRKYLYM LMESCLGGE LWTVLRDKG HFDDATTRF 
      YTACVVEAF DYLHSRNII YRDLKPENL LLDNQGYVK 
      LVDFGFAKK LHSGRKTWT FCGTPEYVA PEVILNKGH 
      DISADYWSL GVLMFELLT GTPPFTGAD PMKTYNIIL 
      KGIDAIDFP RNITRNAMA LIKKLCRDN PAERLGYQK 
      GGISEIQKH KWFDGFNWE GLVNRTLTP PILPQVKHV 
      TDTSNFDDY PPDTDTPPP DDVSGWDAD F
    • >TC034109 RC
      MRVCFGTLC FSSRLTAND EEAAPGLVG AATVTTTTA 
      LNGHHRQAM ASSVDELQA LLAEKETKI QELTKLVQQ 
      KDLEITNLR SQLDKFQSV LPLYNPTSP KHYVGLNNN 
      AVLRPRKQR AGISAEPQS EASILELSK ETFPTYNKD 
      ER
    CDS sequence
    • >TC034109 RA
      atgcgcgtc tgtttcggg acgctgtgc ttctcgtcg 
      aggctgacc gctaacgac gaggaagcc gccccaggt 
      ctcgttggt gccgcgacg gtgacgacg acgacggcc 
      ctcaacggc catcatcgg caagccatg gcgtcgtcc 
      gtcgacgaa ctccaagcg ctcctcgcc gagaaggag 
      accaaaatc caggagttg accaaactg gtgcagcag 
      aaggacctg gagatcact aatctcagg tcgcagctc 
      gacaaattc cagagtgtt ctgccgctc tataacccc 
      acgagtcct aaacactac gtcgggctg aacaataat 
      gccgtctta aggcccagg aagcaaagg gccggcatt 
      tccgcagag ccgcagagc gaggcctcg attctggag 
      ctgagcaaa gagacgttt cctacttac aataaggac 
      gaaagttct cgcgaactc atcaaagct gcaattttg 
      gacaacgac ttcatgaaa aatttggaa agcacccaa 
      atcaaggaa atcgtcgat tgcatgtac ccggaagcg 
      tacacggcc ggaagtctt atcatcaag gaaggggac 
      gtcgggagc atagtctac gtcctggaa gccgtctcc 
      ctactttcc tgcaatttc ctgctgccg ctctcagtc 
      aacgtccac atctcgaag gcgacggtt tcgcgttcg 
      gccccttca ccgtcgacc tcgtgcaac gaaaacgtg 
      aaatgtagt tccgcgtct gaagacatt tccatcaca 
      aagtgtaag atttggtgg agctgctgc caggcccgg 
      aaaaatccg gaagtgaca cattttcgc ccgtttttc 
      aaaccgagt gaaagtatc ggggccacc ggtcagaac 
      acaatgaag atcaaacat tacccgggc aaggcggtg 
      atcaacgat cccgcttgg ctagaaatg gacatttcg 
      aacgacatc aggggacgc cagtatgag gcaaactgg 
      gaagacacc cagcggaat ttatcgtca actaacagt 
      tctgggtcc aattcttgg gacaagtac agcgaagga 
      acgatttcc tcaaaactg gggcattac cagaaggtg 
      ctagatgac ctagcacag gaggaactg tacacacaa 
      cccatcaaa aaagaggag aagcccaaa tatcacagc 
      gagaagtgt ttagcgagg attaacaat aacacagtc 
      ccgaattat tacctgcct tcgcaagcc aagattcag 
      aagagtcgc agttttaaa gaaacggaa gataaacca 
      aaacctaaa cctagaact aaactggcc cagaatctc 
      aaaaacgtg gttaacgat ctagagaag caaatcaag 
      tcggttcgg tttaccgaa gaagaggag gccgagcct 
      gggcaaaga atcagccaa tggattgat gaccagaac 
      aagtacatt ttaactgac agcgtgctg atcaagggc 
      gaggacctg cccaagaag ccgcgccag aggatacct 
      ccaaggaac ctcacgccg tcgccgtcg gaagtcagt 
      gacgagcgt tctagtgcg tacaatttg tacaaaagt 
      ggcgcaaaa gcgcaagtg ctcgaagcg caaaacagt 
      gattttctg attccgcgt ccgaaactg atcgtccct 
      gtgcatacg tacgcggtg aggaggcgg cgcaccggc 
      aacttgcag tgtgccggc gaagaggtc atcaagaac 
      aaagattcg gagggttgc gtcgaagtc tcccgtgaa 
      aacaagttc ctgagcacc ctcacccct ggcaaggtt 
      ctaggcgag ctcgcaatc ctctacaat tgtcaaagg 
      accgcaact atcaaagcc gccaccgac tgcaaacta 
      tgggccatc gaacggcaa tgcttccag acgattatg 
      atgaggacc ggcctcatc agacaagcc gaatacacc 
      gatttcctc aagagcgtc cccatcttc aaaaacctc 
      cccgaagac actctcatc aaaatatcg gacgttctg 
      gaggaaacc ttctacgcc aacggcgac tacatcatc 
      agacagggt gccagaggc gacaccttc ttcatcatc 
      agcaagggc acggtcaag gtgacgaaa aaagtgccc 
      gacagcaac gaagaaaag tacatcagg acgctggga 
      aagggcgac ttcttcggc gaaaaagcc ctccaaggg 
      gatgatctg cgcacggcg aacatcatc gtcgacaac 
      cccgaaggg gtctattgt ctggtcatt gaccgcgag 
      acgttcaac caactgatc tcgaacctg gacgagatc 
      aggaccaag tacaaggac gagggagtg gacaggaga 
      agggaaaac gaagagttc gaacacgtc caactgacc 
      gatttgaaa aaactcacg actttgggc gttggcggg 
      ttcggacgc gtcgaactc gtgcaaatc caaggcgac 
      tcgaaccgc tcgttcgcc ctcaagcag atgaagaag 
      gcacagatc gtggagacg cgtcaacag caacatatc 
      atgtccgag aaggagatt atgggcgag gctaattgc 
      gactttatc gtcaagctg ttcaagacg tttaaagac 
      cgcaaatat ttgtacatg ctgatggag agctgtctg 
      ggaggcgag ctctggacg gtgttgagg gataaggga 
      cactttgac gatgccacg accaggttc tacaccgcc 
      tgcgtggtg gaggcgttc gattatctg cattcccgc 
      aacattatt tacagggac ttgaaaccg gaaaacctc 
      ctcttagac aaccaaggc tacgtgaaa cttgtcgat 
      ttcgggttc gccaagaag ctgcacagc ggccgcaag 
      acctggacg ttctgtgga acccccgaa tacgtggcc 
      cctgaagtg atactgaac aaggggcac gacataagc 
      gccgactat tggtccctt ggcgtgctg atgtttgaa 
      ctgttgacg gggacgccg cccttcacg ggagcggac 
      cccatgaag acctacaac atcatcctg aaaggcata 
      gacgcgata gatttccct cggaacatc acgcgcaac 
      gccatggcc cttatcaag aaactgtgc agagacaac 
      ccggccgag cgcttgggg taccagaaa ggggggatt 
      agcgagata cagaagcac aagtggttc gatggtttc 
      aactgggag ggtctggtg aacaggacg ctgacgccg 
      ccgattttg ccgcaagtc aagcacgtg acggacacg 
      agcaacttc gatgactac cccccggac acggacacc 
      ccgccacct gatgacgtg tcgggctgg gatgccgat 
      ttttag
    • >TC034109 RB
      atgagttgc tacaacttc atcagcaaa gtgctaacg 
      cgtcggaag taccacagc gagatgccg cccagcgaa 
      gcggaggtc gtgcagagc ctgaagaag atgctggag 
      gacaagacg cgcgagctg agcctgaag gaggagacg 
      atcgcgctc ctggagaag gagctggac gacaaggac 
      gccatcatc aagcacttg cacaacgag attgataag 
      ttccgacag gtggtaaaa ccgatcacc cagaaaatc 
      atcaccaag cagctgact ctgggcgat gggaagccc 
      aggaccaag cggcaggcc atcagtgcg gagccgctc 
      aacgacgag ttcggggat ttacagatc aagaaaatc 
      ccgaaatcg gcgacttct cgcgaactc atcaaagct 
      gcaattttg gacaacgac ttcatgaaa aatttggaa 
      agcacccaa atcaaggaa atcgtcgat tgcatgtac 
      ccggaagcg tacacggcc ggaagtctt atcatcaag 
      gaaggggac gtcgggagc atagtctac gtcctggaa 
      gccgtctcc ctactttcc tgcaatttc ctgctgccg 
      ctctcagtc aacgtccac atctcgaag gcgacggtt 
      tcgcgttcg gccccttca ccgtcgacc tcgtgcaac 
      gaaaacgtg aaatgtagt tccgcgtct gaagacatt 
      tccatcaca aagtgtaag atttggtgg agctgctgc 
      caggcccgg aaaaatccg gaagtgaca cattttcgc 
      ccgtttttc aaaccgagt gaaagtatc ggggccacc 
      ggtcagaac acaatgaag atcaaacat tacccgggc 
      aaggcggtg atcaacgat cccgcttgg ctagaaatg 
      gacatttcg aacgacatc aggggacgc cagtatgag 
      gcaaactgg gaagacacc cagcggaat ttatcgtca 
      actaacagt tctgggtcc aattcttgg gacaagtac 
      agcgaagga acgatttcc tcaaaactg gggcattac 
      cagaaggtg ctagatgac ctagcacag gaggaactg 
      tacacacaa cccatcaaa aaagaggag aagcccaaa 
      tatcacagc gagaagtgt ttagcgagg attaacaat 
      aacacagtc ccgaattat tacctgcct tcgcaagcc 
      aagattcag aagagtcgc agttttaaa gaaacggaa 
      gataaacca aaacctaaa cctagaact aaactggcc 
      cagaatctc aaaaacgtg gttaacgat ctagagaag 
      caaatcaag tcggttcgg tttaccgaa gaagaggag 
      gccgagcct gggcaaaga atcagccaa tggattgat 
      gaccagaac aagtacatt ttaactgac agcgtgctg 
      atcaagggc gaggacctg cccaagaag ccgcgccag 
      aggatacct ccaaggaac ctcacgccg tcgccgtcg 
      gaagtcagt gacgagcgt tctagtgcg tacaatttg 
      tacaaaagt ggcgcaaaa gcgcaagtg ctcgaagcg 
      caaaacagt gattttctg attccgcgt ccgaaactg 
      atcgtccct gtgcatacg tacgcggtg aggaggcgg 
      cgcaccggc aacttgcag tgtgccggc gaagaggtc 
      atcaagaac aaagattcg gagggttgc gtcgaagtc 
      tcccgtgaa aacaagttc ctgagcacc ctcacccct 
      ggcaaggtt ctaggcgag ctcgcaatc ctctacaat 
      tgtcaaagg accgcaact atcaaagcc gccaccgac 
      tgcaaacta tgggccatc gaacggcaa tgcttccag 
      acgattatg atgaggacc ggcctcatc agacaagcc 
      gaatacacc gatttcctc aagagcgtc cccatcttc 
      aaaaacctc cccgaagac actctcatc aaaatatcg 
      gacgttctg gaggaaacc ttctacgcc aacggcgac 
      tacatcatc agacagggt gccagaggc gacaccttc 
      ttcatcatc agcaagggc acggtcaag gtgacgaaa 
      aaagtgccc gacagcaac gaagaaaag tacatcagg 
      acgctggga aagggcgac ttcttcggc gaaaaagcc 
      ctccaaggg gatgatctg cgcacggcg aacatcatc 
      gtcgacaac cccgaaggg gtctattgt ctggtcatt 
      gaccgcgag acgttcaac caactgatc tcgaacctg 
      gacgagatc aggaccaag tacaaggac gagggagtg 
      gacaggaga agggaaaac gaagagttc gaacacgtc 
      caactgacc gatttgaaa aaactcacg actttgggc 
      gttggcggg ttcggacgc gtcgaactc gtgcaaatc 
      caaggcgac tcgaaccgc tcgttcgcc ctcaagcag 
      atgaagaag gcacagatc gtggagacg cgtcaacag 
      caacatatc atgtccgag aaggagatt atgggcgag 
      gctaattgc gactttatc gtcaagctg ttcaagacg 
      tttaaagac cgcaaatat ttgtacatg ctgatggag 
      agctgtctg ggaggcgag ctctggacg gtgttgagg 
      gataaggga cactttgac gatgccacg accaggttc 
      tacaccgcc tgcgtggtg gaggcgttc gattatctg 
      cattcccgc aacattatt tacagggac ttgaaaccg 
      gaaaacctc ctcttagac aaccaaggc tacgtgaaa 
      cttgtcgat ttcgggttc gccaagaag ctgcacagc 
      ggccgcaag acctggacg ttctgtgga acccccgaa 
      tacgtggcc cctgaagtg atactgaac aaggggcac 
      gacataagc gccgactat tggtccctt ggcgtgctg 
      atgtttgaa ctgttgacg gggacgccg cccttcacg 
      ggagcggac cccatgaag acctacaac atcatcctg 
      aaaggcata gacgcgata gatttccct cggaacatc 
      acgcgcaac gccatggcc cttatcaag aaactgtgc 
      agagacaac ccggccgag cgcttgggg taccagaaa 
      ggggggatt agcgagata cagaagcac aagtggttc 
      gatggtttc aactgggag ggtctggtg aacaggacg 
      ctgacgccg ccgattttg ccgcaagtc aagcacgtg 
      acggacacg agcaacttc gatgactac cccccggac 
      acggacacc ccgccacct gatgacgtg tcgggctgg 
      gatgccgat ttttag
    • >TC034109 RC
      atgcgcgtc tgtttcggg acgctgtgc ttctcgtcg 
      aggctgacc gctaacgac gaggaagcc gccccaggt 
      ctcgttggt gccgcgacg gtgacgacg acgacggcc 
      ctcaacggc catcatcgg caagccatg gcgtcgtcc 
      gtcgacgaa ctccaagcg ctcctcgcc gagaaggag 
      accaaaatc caggagttg accaaactg gtgcagcag 
      aaggacctg gagatcact aatctcagg tcgcagctc 
      gacaaattc cagagtgtt ctgccgctc tataacccc 
      acgagtcct aaacactac gtcgggctg aacaataat 
      gccgtctta aggcccagg aagcaaagg gccggcatt 
      tccgcagag ccgcagagc gaggcctcg attctggag 
      ctgagcaaa gagacgttt cctacttac aataaggac 
      gaaaggtag
    mRNA sequence
    • >TC034109 RA
      cgctctaaa aatagccgc aatgtccat ccctgattt 
      cattcatcc tgcgacgtc cacaaaacc acttgttat 
      cgagaatgc acacgtggt actcggcgg ggatgggcg 
      aagggggcc ggagggggg ccccagtcg agctcagcc 
      gcagtcgag gttcagttc acgctcatc aaatcgccg 
      taaacaaaa gacaaacgc gaaaacgcg tgtgatcta 
      gtcggcatg tagccgcgt tcatttgtt agatcctag 
      tgcagtgat agtgatttt ggtgtccac gtgttggtg 
      cgtgatcac tgatttcga gccgtgtcc cgcccgcaa 
      tgtgcttcc atctctagt tccgtagcc caagttcgc 
      gtgtttcac accttcatc ctcggtttt cggcgtcgc 
      gacgcgcgt ctttcttcc ccaccgccg tttcgccgc 
      gtgaatgcg cgtctgttt cgggacgct gtgcttctc 
      gtcgaggct gaccgctaa cgacgagga agccgcccc 
      aggtctcgt tggtgccgc gacggtgac gacgacgac 
      ggccctcaa cggccatca tcggcaagc catggcgtc 
      gtccgtcga cgaactcca agcgctcct cgccgagaa 
      ggagaccaa aatccagga gttgaccaa actggtgca 
      gcagaagga cctggagat cactaatct caggtcgca 
      gctcgacaa attccagag tgttctgcc gctctataa 
      ccccacgag tcctaaaca ctacgtcgg gctgaacaa 
      taatgccgt cttaaggcc caggaagca aagggccgg 
      catttccgc agagccgca gagcgaggc ctcgattct 
      ggagctgag caaagagac gtttcctac ttacaataa 
      ggacgaaag ttctcgcga actcatcaa agctgcaat 
      tttggacaa cgacttcat gaaaaattt ggaaagcac 
      ccaaatcaa ggaaatcgt cgattgcat gtacccgga 
      agcgtacac ggccggaag tcttatcat caaggaagg 
      ggacgtcgg gagcatagt ctacgtcct ggaagccgt 
      ctccctact ttcctgcaa tttcctgct gccgctctc 
      agtcaacgt ccacatctc gaaggcgac ggtttcgcg 
      ttcggcccc ttcaccgtc gacctcgtg caacgaaaa 
      cgtgaaatg tagttccgc gtctgaaga catttccat 
      cacaaagtg taagatttg gtggagctg ctgccaggc 
      ccggaaaaa tccggaagt gacacattt tcgcccgtt 
      tttcaaacc gagtgaaag tatcggggc caccggtca 
      gaacacaat gaagatcaa acattaccc gggcaaggc 
      ggtgatcaa cgatcccgc ttggctaga aatggacat 
      ttcgaacga catcagggg acgccagta tgaggcaaa 
      ctgggaaga cacccagcg gaatttatc gtcaactaa 
      cagttctgg gtccaattc ttgggacaa gtacagcga 
      aggaacgat ttcctcaaa actggggca ttaccagaa 
      ggtgctaga tgacctagc acaggagga actgtacac 
      acaacccat caaaaaaga ggagaagcc caaatatca 
      cagcgagaa gtgtttagc gaggattaa caataacac 
      agtcccgaa ttattacct gccttcgca agccaagat 
      tcagaagag tcgcagttt taaagaaac ggaagataa 
      accaaaacc taaacctag aactaaact ggcccagaa 
      tctcaaaaa cgtggttaa cgatctaga gaagcaaat 
      caagtcggt tcggtttac cgaagaaga ggaggccga 
      gcctgggca aagaatcag ccaatggat tgatgacca 
      gaacaagta cattttaac tgacagcgt gctgatcaa 
      gggcgagga cctgcccaa gaagccgcg ccagaggat 
      acctccaag gaacctcac gccgtcgcc gtcggaagt 
      cagtgacga gcgttctag tgcgtacaa tttgtacaa 
      aagtggcgc aaaagcgca agtgctcga agcgcaaaa 
      cagtgattt tctgattcc gcgtccgaa actgatcgt 
      ccctgtgca tacgtacgc ggtgaggag gcggcgcac 
      cggcaactt gcagtgtgc cggcgaaga ggtcatcaa 
      gaacaaaga ttcggaggg ttgcgtcga agtctcccg 
      tgaaaacaa gttcctgag caccctcac ccctggcaa 
      ggttctagg cgagctcgc aatcctcta caattgtca 
      aaggaccgc aactatcaa agccgccac cgactgcaa 
      actatgggc catcgaacg gcaatgctt ccagacgat 
      tatgatgag gaccggcct catcagaca agccgaata 
      caccgattt cctcaagag cgtccccat cttcaaaaa 
      cctccccga agacactct catcaaaat atcggacgt 
      tctggagga aaccttcta cgccaacgg cgactacat 
      catcagaca gggtgccag aggcgacac cttcttcat 
      catcagcaa gggcacggt caaggtgac gaaaaaagt 
      gcccgacag caacgaaga aaagtacat caggacgct 
      gggaaaggg cgacttctt cggcgaaaa agccctcca 
      aggggatga tctgcgcac ggcgaacat catcgtcga 
      caaccccga aggggtcta ttgtctggt cattgaccg 
      cgagacgtt caaccaact gatctcgaa cctggacga 
      gatcaggac caagtacaa ggacgaggg agtggacag 
      gagaaggga aaacgaaga gttcgaaca cgtccaact 
      gaccgattt gaaaaaact cacgacttt gggcgttgg 
      cgggttcgg acgcgtcga actcgtgca aatccaagg 
      cgactcgaa ccgctcgtt cgccctcaa gcagatgaa 
      gaaggcaca gatcgtgga gacgcgtca acagcaaca 
      tatcatgtc cgagaagga gattatggg cgaggctaa 
      ttgcgactt tatcgtcaa gctgttcaa gacgtttaa 
      agaccgcaa atatttgta catgctgat ggagagctg 
      tctgggagg cgagctctg gacggtgtt gagggataa 
      gggacactt tgacgatgc cacgaccag gttctacac 
      cgcctgcgt ggtggaggc gttcgatta tctgcattc 
      ccgcaacat tatttacag ggacttgaa accggaaaa 
      cctcctctt agacaacca aggctacgt gaaacttgt 
      cgatttcgg gttcgccaa gaagctgca cagcggccg 
      caagacctg gacgttctg tggaacccc cgaatacgt 
      ggcccctga agtgatact gaacaaggg gcacgacat 
      aagcgccga ctattggtc ccttggcgt gctgatgtt 
      tgaactgtt gacggggac gccgccctt cacgggagc 
      ggaccccat gaagaccta caacatcat cctgaaagg 
      catagacgc gatagattt ccctcggaa catcacgcg 
      caacgccat ggcccttat caagaaact gtgcagaga 
      caacccggc cgagcgctt ggggtacca gaaaggggg 
      gattagcga gatacagaa gcacaagtg gttcgatgg 
      tttcaactg ggagggtct ggtgaacag gacgctgac 
      gccgccgat tttgccgca agtcaagca cgtgacgga 
      cacgagcaa cttcgatga ctacccccc ggacacgga 
      caccccgcc acctgatga cgtgtcggg ctgggatgc 
      cgattttta gagtaatgt tagtttagg ttgttacaa 
      ttggatatt gatgagagc tgtgggaat tgcaacgca 
      aagtaagca ggtgccaac taacgcagc gttgcaagc 
      gatcgatta gcacatgac aataattct ggttttcga 
      ctgatggtg gagatggcc ccaaaacgc acaagtgcc 
      attttaatt cgttttaaa aaggaattg tttgtatat 
      ttttttaat ttacaagta catgttgtg actgtttat 
      attttaaca gaaagttac ccatttaca ttatgtgta 
      tatttcaat aattgtaaa gcaatccat aatttaata 
      atgtaaagt tttttattg tgtaagttt gtgaatata 
      agattctaa agttg
    • >TC034109 RB
      cgaagctta aaattcatt gcgaaattc cctcgcgta 
      attatcggt gtgtgtgtg cgttgggag aataatttc 
      gcgcaaaag agggtcatg gtactttgc gtaacgtcc 
      cgaaatttc ggtttccgg cagtgtgtg cttaaaatg 
      gacctatga gttgctaca acttcatca gcaaagtgc 
      taacgcgtc ggaagtacc acagcgaga tgccgccca 
      gcgaagcgg aggtcgtgc agagcctga agaagatgc 
      tggaggaca agacgcgcg agctgagcc tgaaggagg 
      agacgatcg cgctcctgg agaaggagc tggacgaca 
      aggacgcca tcatcaagc acttgcaca acgagattg 
      ataagttcc gacaggtgg taaaaccga tcacccaga 
      aaatcatca ccaagcagc tgactctgg gcgatggga 
      agcccagga ccaagcggc aggccatca gtgcggagc 
      cgctcaacg acgagttcg gggatttac agatcaaga 
      aaatcccga aatcggcga cttctcgcg aactcatca 
      aagctgcaa ttttggaca acgacttca tgaaaaatt 
      tggaaagca cccaaatca aggaaatcg tcgattgca 
      tgtacccgg aagcgtaca cggccggaa gtcttatca 
      tcaaggaag gggacgtcg ggagcatag tctacgtcc 
      tggaagccg tctccctac tttcctgca atttcctgc 
      tgccgctct cagtcaacg tccacatct cgaaggcga 
      cggtttcgc gttcggccc cttcaccgt cgacctcgt 
      gcaacgaaa acgtgaaat gtagttccg cgtctgaag 
      acatttcca tcacaaagt gtaagattt ggtggagct 
      gctgccagg cccggaaaa atccggaag tgacacatt 
      ttcgcccgt ttttcaaac cgagtgaaa gtatcgggg 
      ccaccggtc agaacacaa tgaagatca aacattacc 
      cgggcaagg cggtgatca acgatcccg cttggctag 
      aaatggaca tttcgaacg acatcaggg gacgccagt 
      atgaggcaa actgggaag acacccagc ggaatttat 
      cgtcaacta acagttctg ggtccaatt cttgggaca 
      agtacagcg aaggaacga tttcctcaa aactggggc 
      attaccaga aggtgctag atgacctag cacaggagg 
      aactgtaca cacaaccca tcaaaaaag aggagaagc 
      ccaaatatc acagcgaga agtgtttag cgaggatta 
      acaataaca cagtcccga attattacc tgccttcgc 
      aagccaaga ttcagaaga gtcgcagtt ttaaagaaa 
      cggaagata aaccaaaac ctaaaccta gaactaaac 
      tggcccaga atctcaaaa acgtggtta acgatctag 
      agaagcaaa tcaagtcgg ttcggttta ccgaagaag 
      aggaggccg agcctgggc aaagaatca gccaatgga 
      ttgatgacc agaacaagt acattttaa ctgacagcg 
      tgctgatca agggcgagg acctgccca agaagccgc 
      gccagagga tacctccaa ggaacctca cgccgtcgc 
      cgtcggaag tcagtgacg agcgttcta gtgcgtaca 
      atttgtaca aaagtggcg caaaagcgc aagtgctcg 
      aagcgcaaa acagtgatt ttctgattc cgcgtccga 
      aactgatcg tccctgtgc atacgtacg cggtgagga 
      ggcggcgca ccggcaact tgcagtgtg ccggcgaag 
      aggtcatca agaacaaag attcggagg gttgcgtcg 
      aagtctccc gtgaaaaca agttcctga gcaccctca 
      cccctggca aggttctag gcgagctcg caatcctct 
      acaattgtc aaaggaccg caactatca aagccgcca 
      ccgactgca aactatggg ccatcgaac ggcaatgct 
      tccagacga ttatgatga ggaccggcc tcatcagac 
      aagccgaat acaccgatt tcctcaaga gcgtcccca 
      tcttcaaaa acctccccg aagacactc tcatcaaaa 
      tatcggacg ttctggagg aaaccttct acgccaacg 
      gcgactaca tcatcagac agggtgcca gaggcgaca 
      ccttcttca tcatcagca agggcacgg tcaaggtga 
      cgaaaaaag tgcccgaca gcaacgaag aaaagtaca 
      tcaggacgc tgggaaagg gcgacttct tcggcgaaa 
      aagccctcc aaggggatg atctgcgca cggcgaaca 
      tcatcgtcg acaaccccg aaggggtct attgtctgg 
      tcattgacc gcgagacgt tcaaccaac tgatctcga 
      acctggacg agatcagga ccaagtaca aggacgagg 
      gagtggaca ggagaaggg aaaacgaag agttcgaac 
      acgtccaac tgaccgatt tgaaaaaac tcacgactt 
      tgggcgttg gcgggttcg gacgcgtcg aactcgtgc 
      aaatccaag gcgactcga accgctcgt tcgccctca 
      agcagatga agaaggcac agatcgtgg agacgcgtc 
      aacagcaac atatcatgt ccgagaagg agattatgg 
      gcgaggcta attgcgact ttatcgtca agctgttca 
      agacgttta aagaccgca aatatttgt acatgctga 
      tggagagct gtctgggag gcgagctct ggacggtgt 
      tgagggata agggacact ttgacgatg ccacgacca 
      ggttctaca ccgcctgcg tggtggagg cgttcgatt 
      atctgcatt cccgcaaca ttatttaca gggacttga 
      aaccggaaa acctcctct tagacaacc aaggctacg 
      tgaaacttg tcgatttcg ggttcgcca agaagctgc 
      acagcggcc gcaagacct ggacgttct gtggaaccc 
      ccgaatacg tggcccctg aagtgatac tgaacaagg 
      ggcacgaca taagcgccg actattggt cccttggcg 
      tgctgatgt ttgaactgt tgacgggga cgccgccct 
      tcacgggag cggacccca tgaagacct acaacatca 
      tcctgaaag gcatagacg cgatagatt tccctcgga 
      acatcacgc gcaacgcca tggccctta tcaagaaac 
      tgtgcagag acaacccgg ccgagcgct tggggtacc 
      agaaagggg ggattagcg agatacaga agcacaagt 
      ggttcgatg gtttcaact gggagggtc tggtgaaca 
      ggacgctga cgccgccga ttttgccgc aagtcaagc 
      acgtgacgg acacgagca acttcgatg actaccccc 
      cggacacgg acaccccgc cacctgatg acgtgtcgg 
      gctgggatg ccgattttt agagtaatg ttagtttag 
      gttgttaca attggatat tgatgagag ctgtgggaa 
      ttgcaacgc aaagtaagc aggtgccaa ctaacgcag 
      cgttgcaag cgatcgatt agcacatga caataattc 
      tggttttcg actgatggt ggagatggc cccaaaacg 
      cacaagtgc cattttaat tcgttttaa aaaggaatt 
      gtttgtata tttttttaa tttacaagt acatgttgt 
      gactgttta tattttaac agaaagtta cccatttac 
      attatgtgt atatttcaa taattgtaa agcaatcca 
      taatttaat aatgtaaag ttttttatt gtgtaagtt 
      tgtgaatat aagattcta aagttg
    • >TC034109 RC
      cgctctaaa aatagccgc aatgtccat ccctgattt 
      cattcatcc tgcgacgtc cacaaaacc acttgttat 
      cgagaatgc acacgtggt actcggcgg ggatgggcg 
      aagggggcc ggagggggg ccccagtcg agctcagcc 
      gcagtcgag gttcagttc acgctcatc aaatcgccg 
      taaacaaaa gacaaacgc gaaaacgcg tgtgatcta 
      gtcggcatg tagccgcgt tcatttgtt agatcctag 
      tgcagtgat agtgatttt ggtgtccac gtgttggtg 
      cgtgatcac tgatttcga gccgtgtcc cgcccgcaa 
      tgtgcttcc atctctagt tccgtagcc caagttcgc 
      gtgtttcac accttcatc ctcggtttt cggcgtcgc 
      gacgcgcgt ctttcttcc ccaccgccg tttcgccgc 
      gtgaatgcg cgtctgttt cgggacgct gtgcttctc 
      gtcgaggct gaccgctaa cgacgagga agccgcccc 
      aggtctcgt tggtgccgc gacggtgac gacgacgac 
      ggccctcaa cggccatca tcggcaagc catggcgtc 
      gtccgtcga cgaactcca agcgctcct cgccgagaa 
      ggagaccaa aatccagga gttgaccaa actggtgca 
      gcagaagga cctggagat cactaatct caggtcgca 
      gctcgacaa attccagag tgttctgcc gctctataa 
      ccccacgag tcctaaaca ctacgtcgg gctgaacaa 
      taatgccgt cttaaggcc caggaagca aagggccgg 
      catttccgc agagccgca gagcgaggc ctcgattct 
      ggagctgag caaagagac gtttcctac ttacaataa 
      ggacgaaag gtaggaatt gtttattat atcaccttc 
      caacagctt tatttttcg tttctaatt gtaattgtg 
      gcgaaacct gacaaaaaa aaccatgac aggaaatat 
      caaaatgga aaaatgtta aggaaaaaa ttatcggca 
      aaatagtaa agaaaataa ataaataaa aataat
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