Status
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Gene information
General information
TC006507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10710861..10716782
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006507
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04160

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006507 RA
      MDEFPNSDD EFELMYGDD LEVLHEQGD VQRTPRKSR 
      RSLEFTQTP VSHNDNSFS DAFTPLEAP VPELNTNKR 
      SIEELFGDI DDILYENRA NAKRYKGDN ADELALIEH 
      IVELRKLNR ERDAPEIFR KAHVQSIDR AKDNLSYTV 
      PSYPFVGVT RVDGRRLYI RCHSEQYEL EERQKIVQE 
      INLACTLGP NDGLWAEAQ DLILKQATA PDLEQCYQP 
      GDKELWVDL YKPRKYFEL LSDESTNRI MLRWLKLWD 
      KAVFKRRPK IKPIKPAEK NKKMFKAPD LCTDLDEHG 
      RPLHKIALL CGPPGLGKT TLAHMVARH AGYNVVEVN 
      ASDDRSCEA FKTALENAT QMRSVVDQE RRPNCLVFD 
      EIDGAPPSS IDYLVKFVQ GGPSGRKKQ KERNVLKRP 
      IICICNDVY VPALRPLRQ IAFVVNFPP TSNTRLAER 
      LMEIAKWQK VKTDMGAML ALAEKSQND IRACLSVLH 
      FFKSQDKAV TLSDVHKTS VGQKDIQKG IFSVWRDIF 
      LIDKANGDS LKARMSGVL NVVNAFGDY EKIAQGVFE 
      NYPNLKFKN TSMVETCLA LDWFGFSDI VNKSIYSTQ 
      NYNLGSYLP FACVVWHFA FGSRSWQKL QYPSAGYEA 
      RTKQNRQKA IVNELMRGM QPAIRSHVQ PNSLILDIL 
      PLLTTIITP AFRQISLHL YTEEEKKSL DNVVRIMVD 
      YNLSYVQER TADGGYDYK LDPNIDELV SFGPKKSLS 
      YSNKQLIAK EIETAKMRR FEPVQPERS AAPMPNHLQ 
      TLKAKSVCG VEMPVSGQF FSSSMC
    CDS sequence
    • >TC006507 RA
      atggacgag ttccccaat tctgacgat gagttcgag 
      cttatgtac ggcgacgat ctcgaggtg ctccatgag 
      cagggagac gtccaaagg actccaaga aaatcgcgc 
      cgaagtctt gaattcacc caaacccca gtctcccac 
      aacgataac tccttcagt gatgctttc acccccctt 
      gaagccccc gttcccgag cttaacaca aataagcgt 
      tcaattgag gagttgttt ggcgacatt gacgatatt 
      ttgtacgaa aatcgggcg aatgcgaag cgatacaaa 
      ggtgataat gcggacgag ctagcgcta attgagcac 
      atagtcgag ctgcgtaaa ctaaaccgg gagcgtgac 
      gccccggaa attttccgg aaagcccac gttcagagc 
      atagacagg gccaaagac aacctgtcg tacaccgtc 
      ccgagttac cccttcgtg ggggtgacg cgggtcgac 
      ggccgccgg ttgtacatc cggtgccac tccgaacag 
      tacgaactg gaggagcgg caaaaaatc gtccaagaa 
      atcaacctc gcgtgcact ttggggccc aatgacggc 
      ctctgggcc gaggcccaa gacctgatt ttgaaacag 
      gccacagcc cccgacctg gagcagtgc taccaaccc 
      ggcgataaa gaactatgg gttgacttg tacaaaccg 
      cgcaaatat ttcgaactt ttgagcgac gaaagcacc 
      aaccggata atgctgcgt tggctgaag ctttgggac 
      aaggccgtt ttcaagcgg cgccccaaa atcaagcca 
      atcaaaccg gcggaaaaa aataaaaaa atgtttaaa 
      gcccccgat ctttgcacg gatttggac gaacacggg 
      cgtccgctc cacaaaatc gcgctactg tgtgggccc 
      ccagggctc gggaagacc actctggcc cacatggtg 
      gcaaggcat gcgggctat aacgtggtg gaggtgaat 
      gcgtccgat gatcgcagt tgcgaggct tttaagaca 
      gctctggag aacgcgacg caaatgcgc tcggttgtg 
      gatcaggag aggcgcccc aattgtctc gtttttgac 
      gagatcgac ggggcgccc ccttcctct attgactat 
      ctcgtcaag ttcgtgcaa gggggcccc agcggcagg 
      aagaaacag aaggagagg aatgtcttg aaacggccg 
      attatctgc atttgtaac gatgtttac gtgccggcg 
      ctgcgcccc ttgagacaa atcgcgttc gttgtcaat 
      tttccgccc acttctaac acgagactt gccgaacgg 
      ttgatggaa attgcaaag tggcaaaag gtcaagact 
      gacatgggg gccatgcta gccttggcc gagaagtcc 
      caaaatgat attcgggct tgtttgtct gtcctacac 
      ttttttaag tcgcaagac aaggctgtg acgttatca 
      gatgtgcac aagacaagc gtcggacaa aaagacatt 
      caaaagggg attttttcg gtttggcgg gatattttc 
      ctaattgat aaagcgaac ggagacagt ttgaaggcc 
      cgaatgtcg ggggttttg aacgtagtt aacgccttc 
      ggtgactac gaaaaaatc gcccaaggc gtgtttgaa 
      aattacccg aatttgaaa ttcaaaaat acatccatg 
      gttgaaacc tgcctggcc ttggattgg ttcggcttt 
      tccgatatt gtaaacaag tcaatttat tcgacccaa 
      aactacaat ttggggagc tatttgccg ttcgcttgt 
      gtcgtttgg cactttgct ttcgggagc cggagctgg 
      cagaagttg cagtacccg agcgcggga tacgaggcc 
      agaaccaag cagaacaga cagaaagcg atcgtcaat 
      gaattgatg cgtggaatg caaccggcg atacgatcc 
      cacgtccaa ccaaattcc ctaatcctt gacatttta 
      cccctgctc acaacaatc atcacgccg gcgtttaga 
      caaatcagc ctgcatttg tacaccgaa gaagagaaa 
      aagagctta gataacgtg gtcaggatt atggtcgat 
      tacaacctc agctatgtg caggagagg actgcggac 
      gggggatat gactacaag ttggaccct aacatcgac 
      gagctggtg tccttcggc cccaaaaaa tccctctcc 
      tactccaac aagcagctc atcgctaaa gagatcgaa 
      actgcgaaa atgcgccgt ttcgaaccc gtccagcct 
      gagcgttcc gccgccccc atgcccaac cacctccag 
      acgctgaag gcgaagtcc gtgtgcggc gtggaaatg 
      ccggtaagt ggtcaattc ttctcctcg tcgatgtgt 
      taa
    mRNA sequence
    • >TC006507 RA
      cggagtttg tccgtcaag tgcgccaaa tttggcgcg 
      aaaaatagg caatttttg tgcaaaaac cggtgtatt 
      gcccccatg gacgagttc cccaattct gacgatgag 
      ttcgagctt atgtacggc gacgatctc gaggtgctc 
      catgagcag ggagacgtc caaaggact ccaagaaaa 
      tcgcgccga agtcttgaa ttcacccaa accccagtc 
      tcccacaac gataactcc ttcagtgat gctttcacc 
      ccccttgaa gcccccgtt cccgagctt aacacaaat 
      aagcgttca attgaggag ttgtttggc gacattgac 
      gatattttg tacgaaaat cgggcgaat gcgaagcga 
      tacaaaggt gataatgcg gacgagcta gcgctaatt 
      gagcacata gtcgagctg cgtaaacta aaccgggag 
      cgtgacgcc ccggaaatt ttccggaaa gcccacgtt 
      cagagcata gacagggcc aaagacaac ctgtcgtac 
      accgtcccg agttacccc ttcgtgggg gtgacgcgg 
      gtcgacggc cgccggttg tacatccgg tgccactcc 
      gaacagtac gaactggag gagcggcaa aaaatcgtc 
      caagaaatc aacctcgcg tgcactttg gggcccaat 
      gacggcctc tgggccgag gcccaagac ctgattttg 
      aaacaggcc acagccccc gacctggag cagtgctac 
      caacccggc gataaagaa ctatgggtt gacttgtac 
      aaaccgcgc aaatatttc gaacttttg agcgacgaa 
      agcaccaac cggataatg ctgcgttgg ctgaagctt 
      tgggacaag gccgttttc aagcggcgc cccaaaatc 
      aagccaatc aaaccggcg gaaaaaaat aaaaaaatg 
      tttaaagcc cccgatctt tgcacggat ttggacgaa 
      cacgggcgt ccgctccac aaaatcgcg ctactgtgt 
      gggccccca gggctcggg aagaccact ctggcccac 
      atggtggca aggcatgcg ggctataac gtggtggag 
      gtgaatgcg tccgatgat cgcagttgc gaggctttt 
      aagacagct ctggagaac gcgacgcaa atgcgctcg 
      gttgtggat caggagagg cgccccaat tgtctcgtt 
      tttgacgag atcgacggg gcgccccct tcctctatt 
      gactatctc gtcaagttc gtgcaaggg ggccccagc 
      ggcaggaag aaacagaag gagaggaat gtcttgaaa 
      cggccgatt atctgcatt tgtaacgat gtttacgtg 
      ccggcgctg cgccccttg agacaaatc gcgttcgtt 
      gtcaatttt ccgcccact tctaacacg agacttgcc 
      gaacggttg atggaaatt gcaaagtgg caaaaggtc 
      aagactgac atgggggcc atgctagcc ttggccgag 
      aagtcccaa aatgatatt cgggcttgt ttgtctgtc 
      ctacacttt tttaagtcg caagacaag gctgtgacg 
      ttatcagat gtgcacaag acaagcgtc ggacaaaaa 
      gacattcaa aaggggatt ttttcggtt tggcgggat 
      attttccta attgataaa gcgaacgga gacagtttg 
      aaggcccga atgtcgggg gttttgaac gtagttaac 
      gccttcggt gactacgaa aaaatcgcc caaggcgtg 
      tttgaaaat tacccgaat ttgaaattc aaaaataca 
      tccatggtt gaaacctgc ctggccttg gattggttc 
      ggcttttcc gatattgta aacaagtca atttattcg 
      acccaaaac tacaatttg gggagctat ttgccgttc 
      gcttgtgtc gtttggcac tttgctttc gggagccgg 
      agctggcag aagttgcag tacccgagc gcgggatac 
      gaggccaga accaagcag aacagacag aaagcgatc 
      gtcaatgaa ttgatgcgt ggaatgcaa ccggcgata 
      cgatcccac gtccaacca aattcccta atccttgac 
      attttaccc ctgctcaca acaatcatc acgccggcg 
      tttagacaa atcagcctg catttgtac accgaagaa 
      gagaaaaag agcttagat aacgtggtc aggattatg 
      gtcgattac aacctcagc tatgtgcag gagaggact 
      gcggacggg ggatatgac tacaagttg gaccctaac 
      atcgacgag ctggtgtcc ttcggcccc aaaaaatcc 
      ctctcctac tccaacaag cagctcatc gctaaagag 
      atcgaaact gcgaaaatg cgccgtttc gaacccgtc 
      cagcctgag cgttccgcc gcccccatg cccaaccac 
      ctccagacg ctgaaggcg aagtccgtg tgcggcgtg 
      gaaatgccg gtaagtggt caattcttc tcctcgtcg 
      atgtgttaa ctccctcta aattgcttc cgcgcttaa 
      tggattttt tgggtagat gttgtgaag ttgcgactg 
      ggcggaaat gaaataact cgtaagaag ttagccctc 
      ccgtccaga cacgaaaaa ataaaaaca ttataaatc 
      t
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