| General information | |||
| TC006507 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 10710861..10716782 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006507 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04160 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC006507 RA
MDEFPNSDD EFELMYGDD LEVLHEQGD VQRTPRKSR RSLEFTQTP VSHNDNSFS DAFTPLEAP VPELNTNKR SIEELFGDI DDILYENRA NAKRYKGDN ADELALIEH IVELRKLNR ERDAPEIFR KAHVQSIDR AKDNLSYTV PSYPFVGVT RVDGRRLYI RCHSEQYEL EERQKIVQE INLACTLGP NDGLWAEAQ DLILKQATA PDLEQCYQP GDKELWVDL YKPRKYFEL LSDESTNRI MLRWLKLWD KAVFKRRPK IKPIKPAEK NKKMFKAPD LCTDLDEHG RPLHKIALL CGPPGLGKT TLAHMVARH AGYNVVEVN ASDDRSCEA FKTALENAT QMRSVVDQE RRPNCLVFD EIDGAPPSS IDYLVKFVQ GGPSGRKKQ KERNVLKRP IICICNDVY VPALRPLRQ IAFVVNFPP TSNTRLAER LMEIAKWQK VKTDMGAML ALAEKSQND IRACLSVLH FFKSQDKAV TLSDVHKTS VGQKDIQKG IFSVWRDIF LIDKANGDS LKARMSGVL NVVNAFGDY EKIAQGVFE NYPNLKFKN TSMVETCLA LDWFGFSDI VNKSIYSTQ NYNLGSYLP FACVVWHFA FGSRSWQKL QYPSAGYEA RTKQNRQKA IVNELMRGM QPAIRSHVQ PNSLILDIL PLLTTIITP AFRQISLHL YTEEEKKSL DNVVRIMVD YNLSYVQER TADGGYDYK LDPNIDELV SFGPKKSLS YSNKQLIAK EIETAKMRR FEPVQPERS AAPMPNHLQ TLKAKSVCG VEMPVSGQF FSSSMC
- >TC006507 RA
atggacgag ttccccaat tctgacgat gagttcgag cttatgtac ggcgacgat ctcgaggtg ctccatgag cagggagac gtccaaagg actccaaga aaatcgcgc cgaagtctt gaattcacc caaacccca gtctcccac aacgataac tccttcagt gatgctttc acccccctt gaagccccc gttcccgag cttaacaca aataagcgt tcaattgag gagttgttt ggcgacatt gacgatatt ttgtacgaa aatcgggcg aatgcgaag cgatacaaa ggtgataat gcggacgag ctagcgcta attgagcac atagtcgag ctgcgtaaa ctaaaccgg gagcgtgac gccccggaa attttccgg aaagcccac gttcagagc atagacagg gccaaagac aacctgtcg tacaccgtc ccgagttac cccttcgtg ggggtgacg cgggtcgac ggccgccgg ttgtacatc cggtgccac tccgaacag tacgaactg gaggagcgg caaaaaatc gtccaagaa atcaacctc gcgtgcact ttggggccc aatgacggc ctctgggcc gaggcccaa gacctgatt ttgaaacag gccacagcc cccgacctg gagcagtgc taccaaccc ggcgataaa gaactatgg gttgacttg tacaaaccg cgcaaatat ttcgaactt ttgagcgac gaaagcacc aaccggata atgctgcgt tggctgaag ctttgggac aaggccgtt ttcaagcgg cgccccaaa atcaagcca atcaaaccg gcggaaaaa aataaaaaa atgtttaaa gcccccgat ctttgcacg gatttggac gaacacggg cgtccgctc cacaaaatc gcgctactg tgtgggccc ccagggctc gggaagacc actctggcc cacatggtg gcaaggcat gcgggctat aacgtggtg gaggtgaat gcgtccgat gatcgcagt tgcgaggct tttaagaca gctctggag aacgcgacg caaatgcgc tcggttgtg gatcaggag aggcgcccc aattgtctc gtttttgac gagatcgac ggggcgccc ccttcctct attgactat ctcgtcaag ttcgtgcaa gggggcccc agcggcagg aagaaacag aaggagagg aatgtcttg aaacggccg attatctgc atttgtaac gatgtttac gtgccggcg ctgcgcccc ttgagacaa atcgcgttc gttgtcaat tttccgccc acttctaac acgagactt gccgaacgg ttgatggaa attgcaaag tggcaaaag gtcaagact gacatgggg gccatgcta gccttggcc gagaagtcc caaaatgat attcgggct tgtttgtct gtcctacac ttttttaag tcgcaagac aaggctgtg acgttatca gatgtgcac aagacaagc gtcggacaa aaagacatt caaaagggg attttttcg gtttggcgg gatattttc ctaattgat aaagcgaac ggagacagt ttgaaggcc cgaatgtcg ggggttttg aacgtagtt aacgccttc ggtgactac gaaaaaatc gcccaaggc gtgtttgaa aattacccg aatttgaaa ttcaaaaat acatccatg gttgaaacc tgcctggcc ttggattgg ttcggcttt tccgatatt gtaaacaag tcaatttat tcgacccaa aactacaat ttggggagc tatttgccg ttcgcttgt gtcgtttgg cactttgct ttcgggagc cggagctgg cagaagttg cagtacccg agcgcggga tacgaggcc agaaccaag cagaacaga cagaaagcg atcgtcaat gaattgatg cgtggaatg caaccggcg atacgatcc cacgtccaa ccaaattcc ctaatcctt gacatttta cccctgctc acaacaatc atcacgccg gcgtttaga caaatcagc ctgcatttg tacaccgaa gaagagaaa aagagctta gataacgtg gtcaggatt atggtcgat tacaacctc agctatgtg caggagagg actgcggac gggggatat gactacaag ttggaccct aacatcgac gagctggtg tccttcggc cccaaaaaa tccctctcc tactccaac aagcagctc atcgctaaa gagatcgaa actgcgaaa atgcgccgt ttcgaaccc gtccagcct gagcgttcc gccgccccc atgcccaac cacctccag acgctgaag gcgaagtcc gtgtgcggc gtggaaatg ccggtaagt ggtcaattc ttctcctcg tcgatgtgt taa
- >TC006507 RA
cggagtttg tccgtcaag tgcgccaaa tttggcgcg aaaaatagg caatttttg tgcaaaaac cggtgtatt gcccccatg gacgagttc cccaattct gacgatgag ttcgagctt atgtacggc gacgatctc gaggtgctc catgagcag ggagacgtc caaaggact ccaagaaaa tcgcgccga agtcttgaa ttcacccaa accccagtc tcccacaac gataactcc ttcagtgat gctttcacc ccccttgaa gcccccgtt cccgagctt aacacaaat aagcgttca attgaggag ttgtttggc gacattgac gatattttg tacgaaaat cgggcgaat gcgaagcga tacaaaggt gataatgcg gacgagcta gcgctaatt gagcacata gtcgagctg cgtaaacta aaccgggag cgtgacgcc ccggaaatt ttccggaaa gcccacgtt cagagcata gacagggcc aaagacaac ctgtcgtac accgtcccg agttacccc ttcgtgggg gtgacgcgg gtcgacggc cgccggttg tacatccgg tgccactcc gaacagtac gaactggag gagcggcaa aaaatcgtc caagaaatc aacctcgcg tgcactttg gggcccaat gacggcctc tgggccgag gcccaagac ctgattttg aaacaggcc acagccccc gacctggag cagtgctac caacccggc gataaagaa ctatgggtt gacttgtac aaaccgcgc aaatatttc gaacttttg agcgacgaa agcaccaac cggataatg ctgcgttgg ctgaagctt tgggacaag gccgttttc aagcggcgc cccaaaatc aagccaatc aaaccggcg gaaaaaaat aaaaaaatg tttaaagcc cccgatctt tgcacggat ttggacgaa cacgggcgt ccgctccac aaaatcgcg ctactgtgt gggccccca gggctcggg aagaccact ctggcccac atggtggca aggcatgcg ggctataac gtggtggag gtgaatgcg tccgatgat cgcagttgc gaggctttt aagacagct ctggagaac gcgacgcaa atgcgctcg gttgtggat caggagagg cgccccaat tgtctcgtt tttgacgag atcgacggg gcgccccct tcctctatt gactatctc gtcaagttc gtgcaaggg ggccccagc ggcaggaag aaacagaag gagaggaat gtcttgaaa cggccgatt atctgcatt tgtaacgat gtttacgtg ccggcgctg cgccccttg agacaaatc gcgttcgtt gtcaatttt ccgcccact tctaacacg agacttgcc gaacggttg atggaaatt gcaaagtgg caaaaggtc aagactgac atgggggcc atgctagcc ttggccgag aagtcccaa aatgatatt cgggcttgt ttgtctgtc ctacacttt tttaagtcg caagacaag gctgtgacg ttatcagat gtgcacaag acaagcgtc ggacaaaaa gacattcaa aaggggatt ttttcggtt tggcgggat attttccta attgataaa gcgaacgga gacagtttg aaggcccga atgtcgggg gttttgaac gtagttaac gccttcggt gactacgaa aaaatcgcc caaggcgtg tttgaaaat tacccgaat ttgaaattc aaaaataca tccatggtt gaaacctgc ctggccttg gattggttc ggcttttcc gatattgta aacaagtca atttattcg acccaaaac tacaatttg gggagctat ttgccgttc gcttgtgtc gtttggcac tttgctttc gggagccgg agctggcag aagttgcag tacccgagc gcgggatac gaggccaga accaagcag aacagacag aaagcgatc gtcaatgaa ttgatgcgt ggaatgcaa ccggcgata cgatcccac gtccaacca aattcccta atccttgac attttaccc ctgctcaca acaatcatc acgccggcg tttagacaa atcagcctg catttgtac accgaagaa gagaaaaag agcttagat aacgtggtc aggattatg gtcgattac aacctcagc tatgtgcag gagaggact gcggacggg ggatatgac tacaagttg gaccctaac atcgacgag ctggtgtcc ttcggcccc aaaaaatcc ctctcctac tccaacaag cagctcatc gctaaagag atcgaaact gcgaaaatg cgccgtttc gaacccgtc cagcctgag cgttccgcc gcccccatg cccaaccac ctccagacg ctgaaggcg aagtccgtg tgcggcgtg gaaatgccg gtaagtggt caattcttc tcctcgtcg atgtgttaa ctccctcta aattgcttc cgcgcttaa tggattttt tgggtagat gttgtgaag ttgcgactg ggcggaaat gaaataact cgtaagaag ttagccctc ccgtccaga cacgaaaaa ataaaaaca ttataaatc t
