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Gene information
General information
TC006507 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10710861..10716782
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006507
    iBeetle screen iB_04160

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006507 RA
      MDEFPNSDD EFELMYGDD LEVLHEQGD VQRTPRKSR 
RSLEFTQTP VSHNDNSFS DAFTPLEAP VPELNTNKR 
SIEELFGDI DDILYENRA NAKRYKGDN ADELALIEH 
IVELRKLNR ERDAPEIFR KAHVQSIDR AKDNLSYTV 
PSYPFVGVT RVDGRRLYI RCHSEQYEL EERQKIVQE 
INLACTLGP NDGLWAEAQ DLILKQATA PDLEQCYQP 
GDKELWVDL YKPRKYFEL LSDESTNRI MLRWLKLWD 
KAVFKRRPK IKPIKPAEK NKKMFKAPD LCTDLDEHG 
RPLHKIALL CGPPGLGKT TLAHMVARH AGYNVVEVN 
ASDDRSCEA FKTALENAT QMRSVVDQE RRPNCLVFD 
EIDGAPPSS IDYLVKFVQ GGPSGRKKQ KERNVLKRP 
IICICNDVY VPALRPLRQ IAFVVNFPP TSNTRLAER 
LMEIAKWQK VKTDMGAML ALAEKSQND IRACLSVLH 
FFKSQDKAV TLSDVHKTS VGQKDIQKG IFSVWRDIF 
LIDKANGDS LKARMSGVL NVVNAFGDY EKIAQGVFE 
NYPNLKFKN TSMVETCLA LDWFGFSDI VNKSIYSTQ 
NYNLGSYLP FACVVWHFA FGSRSWQKL QYPSAGYEA 
RTKQNRQKA IVNELMRGM QPAIRSHVQ PNSLILDIL 
PLLTTIITP AFRQISLHL YTEEEKKSL DNVVRIMVD 
YNLSYVQER TADGGYDYK LDPNIDELV SFGPKKSLS 
YSNKQLIAK EIETAKMRR FEPVQPERS AAPMPNHLQ 
TLKAKSVCG VEMPVSGQF FSSSMC
    CDS sequence
    • >TC006507 RA
      atggacgag ttccccaat tctgacgat gagttcgag 
cttatgtac ggcgacgat ctcgaggtg ctccatgag 
cagggagac gtccaaagg actccaaga aaatcgcgc 
cgaagtctt gaattcacc caaacccca gtctcccac 
aacgataac tccttcagt gatgctttc acccccctt 
gaagccccc gttcccgag cttaacaca aataagcgt 
tcaattgag gagttgttt ggcgacatt gacgatatt 
ttgtacgaa aatcgggcg aatgcgaag cgatacaaa 
ggtgataat gcggacgag ctagcgcta attgagcac 
atagtcgag ctgcgtaaa ctaaaccgg gagcgtgac 
gccccggaa attttccgg aaagcccac gttcagagc 
atagacagg gccaaagac aacctgtcg tacaccgtc 
ccgagttac cccttcgtg ggggtgacg cgggtcgac 
ggccgccgg ttgtacatc cggtgccac tccgaacag 
tacgaactg gaggagcgg caaaaaatc gtccaagaa 
atcaacctc gcgtgcact ttggggccc aatgacggc 
ctctgggcc gaggcccaa gacctgatt ttgaaacag 
gccacagcc cccgacctg gagcagtgc taccaaccc 
ggcgataaa gaactatgg gttgacttg tacaaaccg 
cgcaaatat ttcgaactt ttgagcgac gaaagcacc 
aaccggata atgctgcgt tggctgaag ctttgggac 
aaggccgtt ttcaagcgg cgccccaaa atcaagcca 
atcaaaccg gcggaaaaa aataaaaaa atgtttaaa 
gcccccgat ctttgcacg gatttggac gaacacggg 
cgtccgctc cacaaaatc gcgctactg tgtgggccc 
ccagggctc gggaagacc actctggcc cacatggtg 
gcaaggcat gcgggctat aacgtggtg gaggtgaat 
gcgtccgat gatcgcagt tgcgaggct tttaagaca 
gctctggag aacgcgacg caaatgcgc tcggttgtg 
gatcaggag aggcgcccc aattgtctc gtttttgac 
gagatcgac ggggcgccc ccttcctct attgactat 
ctcgtcaag ttcgtgcaa gggggcccc agcggcagg 
aagaaacag aaggagagg aatgtcttg aaacggccg 
attatctgc atttgtaac gatgtttac gtgccggcg 
ctgcgcccc ttgagacaa atcgcgttc gttgtcaat 
tttccgccc acttctaac acgagactt gccgaacgg 
ttgatggaa attgcaaag tggcaaaag gtcaagact 
gacatgggg gccatgcta gccttggcc gagaagtcc 
caaaatgat attcgggct tgtttgtct gtcctacac 
ttttttaag tcgcaagac aaggctgtg acgttatca 
gatgtgcac aagacaagc gtcggacaa aaagacatt 
caaaagggg attttttcg gtttggcgg gatattttc 
ctaattgat aaagcgaac ggagacagt ttgaaggcc 
cgaatgtcg ggggttttg aacgtagtt aacgccttc 
ggtgactac gaaaaaatc gcccaaggc gtgtttgaa 
aattacccg aatttgaaa ttcaaaaat acatccatg 
gttgaaacc tgcctggcc ttggattgg ttcggcttt 
tccgatatt gtaaacaag tcaatttat tcgacccaa 
aactacaat ttggggagc tatttgccg ttcgcttgt 
gtcgtttgg cactttgct ttcgggagc cggagctgg 
cagaagttg cagtacccg agcgcggga tacgaggcc 
agaaccaag cagaacaga cagaaagcg atcgtcaat 
gaattgatg cgtggaatg caaccggcg atacgatcc 
cacgtccaa ccaaattcc ctaatcctt gacatttta 
cccctgctc acaacaatc atcacgccg gcgtttaga 
caaatcagc ctgcatttg tacaccgaa gaagagaaa 
aagagctta gataacgtg gtcaggatt atggtcgat 
tacaacctc agctatgtg caggagagg actgcggac 
gggggatat gactacaag ttggaccct aacatcgac 
gagctggtg tccttcggc cccaaaaaa tccctctcc 
tactccaac aagcagctc atcgctaaa gagatcgaa 
actgcgaaa atgcgccgt ttcgaaccc gtccagcct 
gagcgttcc gccgccccc atgcccaac cacctccag 
acgctgaag gcgaagtcc gtgtgcggc gtggaaatg 
ccggtaagt ggtcaattc ttctcctcg tcgatgtgt 
taa
    mRNA sequence
    • >TC006507 RA
      cggagtttg tccgtcaag tgcgccaaa tttggcgcg 
aaaaatagg caatttttg tgcaaaaac cggtgtatt 
gcccccatg gacgagttc cccaattct gacgatgag 
ttcgagctt atgtacggc gacgatctc gaggtgctc 
catgagcag ggagacgtc caaaggact ccaagaaaa 
tcgcgccga agtcttgaa ttcacccaa accccagtc 
tcccacaac gataactcc ttcagtgat gctttcacc 
ccccttgaa gcccccgtt cccgagctt aacacaaat 
aagcgttca attgaggag ttgtttggc gacattgac 
gatattttg tacgaaaat cgggcgaat gcgaagcga 
tacaaaggt gataatgcg gacgagcta gcgctaatt 
gagcacata gtcgagctg cgtaaacta aaccgggag 
cgtgacgcc ccggaaatt ttccggaaa gcccacgtt 
cagagcata gacagggcc aaagacaac ctgtcgtac 
accgtcccg agttacccc ttcgtgggg gtgacgcgg 
gtcgacggc cgccggttg tacatccgg tgccactcc 
gaacagtac gaactggag gagcggcaa aaaatcgtc 
caagaaatc aacctcgcg tgcactttg gggcccaat 
gacggcctc tgggccgag gcccaagac ctgattttg 
aaacaggcc acagccccc gacctggag cagtgctac 
caacccggc gataaagaa ctatgggtt gacttgtac 
aaaccgcgc aaatatttc gaacttttg agcgacgaa 
agcaccaac cggataatg ctgcgttgg ctgaagctt 
tgggacaag gccgttttc aagcggcgc cccaaaatc 
aagccaatc aaaccggcg gaaaaaaat aaaaaaatg 
tttaaagcc cccgatctt tgcacggat ttggacgaa 
cacgggcgt ccgctccac aaaatcgcg ctactgtgt 
gggccccca gggctcggg aagaccact ctggcccac 
atggtggca aggcatgcg ggctataac gtggtggag 
gtgaatgcg tccgatgat cgcagttgc gaggctttt 
aagacagct ctggagaac gcgacgcaa atgcgctcg 
gttgtggat caggagagg cgccccaat tgtctcgtt 
tttgacgag atcgacggg gcgccccct tcctctatt 
gactatctc gtcaagttc gtgcaaggg ggccccagc 
ggcaggaag aaacagaag gagaggaat gtcttgaaa 
cggccgatt atctgcatt tgtaacgat gtttacgtg 
ccggcgctg cgccccttg agacaaatc gcgttcgtt 
gtcaatttt ccgcccact tctaacacg agacttgcc 
gaacggttg atggaaatt gcaaagtgg caaaaggtc 
aagactgac atgggggcc atgctagcc ttggccgag 
aagtcccaa aatgatatt cgggcttgt ttgtctgtc 
ctacacttt tttaagtcg caagacaag gctgtgacg 
ttatcagat gtgcacaag acaagcgtc ggacaaaaa 
gacattcaa aaggggatt ttttcggtt tggcgggat 
attttccta attgataaa gcgaacgga gacagtttg 
aaggcccga atgtcgggg gttttgaac gtagttaac 
gccttcggt gactacgaa aaaatcgcc caaggcgtg 
tttgaaaat tacccgaat ttgaaattc aaaaataca 
tccatggtt gaaacctgc ctggccttg gattggttc 
ggcttttcc gatattgta aacaagtca atttattcg 
acccaaaac tacaatttg gggagctat ttgccgttc 
gcttgtgtc gtttggcac tttgctttc gggagccgg 
agctggcag aagttgcag tacccgagc gcgggatac 
gaggccaga accaagcag aacagacag aaagcgatc 
gtcaatgaa ttgatgcgt ggaatgcaa ccggcgata 
cgatcccac gtccaacca aattcccta atccttgac 
attttaccc ctgctcaca acaatcatc acgccggcg 
tttagacaa atcagcctg catttgtac accgaagaa 
gagaaaaag agcttagat aacgtggtc aggattatg 
gtcgattac aacctcagc tatgtgcag gagaggact 
gcggacggg ggatatgac tacaagttg gaccctaac 
atcgacgag ctggtgtcc ttcggcccc aaaaaatcc 
ctctcctac tccaacaag cagctcatc gctaaagag 
atcgaaact gcgaaaatg cgccgtttc gaacccgtc 
cagcctgag cgttccgcc gcccccatg cccaaccac 
ctccagacg ctgaaggcg aagtccgtg tgcggcgtg 
gaaatgccg gtaagtggt caattcttc tcctcgtcg 
atgtgttaa ctccctcta aattgcttc cgcgcttaa 
tggattttt tgggtagat gttgtgaag ttgcgactg 
ggcggaaat gaaataact cgtaagaag ttagccctc 
ccgtccaga cacgaaaaa ataaaaaca ttataaatc 
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    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY