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Gene information
General information
TC006506 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10705011..10706430
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006506
    iBeetle screen iB_04159

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006506 RA
      MPNVFILVP LVILLWISL LLLLKNSLN TSIQLDINT 
      STNTLAKIA TKIPNRIEN TVPVNKKKY HVPKFMLEL 
      YEQNKVKRD NLTRPDVVR SLTPKHAGP IDDNEVLEE 
      SENHLLIFD LPNVDEEEQ FVSAELKIL TLVEVKSGV 
      ERQLVVSIY DDVTKKFHH LEQIHVYHM NDTWISFNV 
      TQPVVKILQ KTQGQKYLK VVISVRAFL SYYKAPKSD 
      FKLSLMPVE DDFDHDYPI LLLSYTSNN KNITRRKKR 
      SLEDDYEEE TNKLWDDDT TKKIQAKKM KKVKNSCRR 
      KPLYINFSE INFDLWILQ PPGYEAYQC QGKCFYPVA 
      EHLNPTKHA IVQALLHSV SPSKVSRSC CVPTNLDSI 
      SILYVDNNG VLTYRYAYK DMVVLECGC R
    CDS sequence
    • >TC006506 RA
      atgccaaac gtttttatt ctagtgccc ttggtaata 
      ctcctgtgg atttcttta ctattatta ctaaaaaat 
      agcctaaac acttcgatc caactggac ataaacacc 
      agcacgaac acactcgct aaaatcgcc acaaaaata 
      ccaaacaga atcgaaaac actgttccg gtgaacaag 
      aagaaatac cacgttccg aagtttatg ctagaatta 
      tacgaacaa aataaggtg aagagggac aacttgacg 
      cgtcctgac gtcgtccgc agcctaact ccgaaacat 
      gctgggccg attgacgac aatgaagtt cttgaagaa 
      tccgaaaat cacttgctc attttcgat ttgccaaac 
      gtggacgaa gaggaacag tttgtaagt gccgagctg 
      aaaatcctg actttggtt gaggtcaaa tcaggggtt 
      gagcgacaa ctcgtagtt tcaatctac gacgatgtg 
      actaaaaag tttcaccac ctggagcaa atccatgtt 
      taccacatg aacgacacg tggatttct ttcaacgtt 
      acgcaacca gtcgtcaaa attttgcaa aaaacgcaa 
      gggcagaag tatctgaaa gtggtaatt tcggtgcgc 
      gcgtttttg tcctattac aaggcgcct aagagtgac 
      tttaaactg tctctaatg ccagtcgaa gatgatttt 
      gaccacgat tacccaatc ctcttactg tcttacact 
      tcaaataat aaaaatata acacgacgg aaaaagcgc 
      agcttggag gatgactat gaagaagag acgaacaaa 
      ctgtgggac gacgacacg accaaaaaa atccaagca 
      aagaagatg aaaaaagtg aagaatagt tgtcgccgc 
      aagcccctc tatatcaat ttctctgaa atcaatttc 
      gatttgtgg attctccaa cctccaggt tacgaggcg 
      taccagtgc caaggcaag tgtttctac cccgtggct 
      gagcacttg aaccccacc aagcacgcc atagtgcag 
      gcactcctc cacagcgtg tctccatca aaggtttcg 
      aggtcttgc tgcgtccca accaacttg gactcgatt 
      tcaatcctc tacgtcgac aataacggc gttctcact 
      taccgttac gcttacaag gacatggtg gtgttagag 
      tgtggttgt agataa
    mRNA sequence
    • >TC006506 RA
      gttattttt aacaggtat aattctttg ataagacca 
      acaagtccg ataaacacc taaatggat ctcaagtgt 
      tttcccgga cgccgtcgt ctcggcgct agttcgaag 
      atgctcgcg ttttgaaca acaacaaca atgccaaac 
      gtttttatt ctagtgccc ttggtaata ctcctgtgg 
      atttcttta ctattatta ctaaaaaat agcctaaac 
      acttcgatc caactggac ataaacacc agcacgaac 
      acactcgct aaaatcgcc acaaaaata ccaaacaga 
      atcgaaaac actgttccg gtgaacaag aagaaatac 
      cacgttccg aagtttatg ctagaatta tacgaacaa 
      aataaggtg aagagggac aacttgacg cgtcctgac 
      gtcgtccgc agcctaact ccgaaacat gctgggccg 
      attgacgac aatgaagtt cttgaagaa tccgaaaat 
      cacttgctc attttcgat ttgccaaac gtggacgaa 
      gaggaacag tttgtaagt gccgagctg aaaatcctg 
      actttggtt gaggtcaaa tcaggggtt gagcgacaa 
      ctcgtagtt tcaatctac gacgatgtg actaaaaag 
      tttcaccac ctggagcaa atccatgtt taccacatg 
      aacgacacg tggatttct ttcaacgtt acgcaacca 
      gtcgtcaaa attttgcaa aaaacgcaa gggcagaag 
      tatctgaaa gtggtaatt tcggtgcgc gcgtttttg 
      tcctattac aaggcgcct aagagtgac tttaaactg 
      tctctaatg ccagtcgaa gatgatttt gaccacgat 
      tacccaatc ctcttactg tcttacact tcaaataat 
      aaaaatata acacgacgg aaaaagcgc agcttggag 
      gatgactat gaagaagag acgaacaaa ctgtgggac 
      gacgacacg accaaaaaa atccaagca aagaagatg 
      aaaaaagtg aagaatagt tgtcgccgc aagcccctc 
      tatatcaat ttctctgaa atcaatttc gatttgtgg 
      attctccaa cctccaggt tacgaggcg taccagtgc 
      caaggcaag tgtttctac cccgtggct gagcacttg 
      aaccccacc aagcacgcc atagtgcag gcactcctc 
      cacagcgtg tctccatca aaggtttcg aggtcttgc 
      tgcgtccca accaacttg gactcgatt tcaatcctc 
      tacgtcgac aataacggc gttctcact taccgttac 
      gcttacaag gacatggtg gtgttagag tgtggttgt 
      agataagtt cggtaataa atatattgt gagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY