Status
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Gene information
General information
TC006498 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10604690..10616479
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006498
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04158

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006498 RA
      MDIFEELGV PKKYLKPTT KEGHYIALV HKSWLKSLV 
      KYSKLVEFA ERGRFDAWP VSEEELGAS WSKVFICVF 
      KKRDASVIP LPRIRSLIK DDPPLLFCQ LMQYIYQTN 
      YKNLWKEAY KKYNSKTET SKETQISLV EYNEVLREI 
      LTRIYGCPL VNICDSRLS TEQSKNFDV HLNVLPAVC 
      AVETLNAIF VLHMPYFDH SLKDCVTFS PAILNKCYA 
      KPLFIIYQL LQTLKSMHD RSLTLGDIS LSDIYLTED 
      MWIYIIPNI HSNIYVQET KTEAKRHVN IPDCRKNGH 
      KFDLNLKCE GCGIKTYDK VQISNETLQ ELCQLWVEG 
      QISNFTYIS ALNKLSGRK LGDPNCHHV FPWVTDFAS 
      RCGKNWRDL KKSKFRLNK GDRQLDLTY ENASSEVPH 
      HVSDVLSSI TYYVYMARR TPKSVLCKN VRTIWVPAE 
      YPSSIQRMQ EWTPDECIP EFFTDPTVF KSIHDDLDD 
      LEVPSWCTG PEDFVEKHR EALESVHVS ERLHYWIDL 
      TFGYKLAGA AAVKAKNVC LHLVDDHTK LMKSGIVQL 
      FTHPHPPKA TPSPFWNKT PPKIYISKT KPKSRDRSN 
      SPGLTLDAK TDDEDIEDI PASSNRPLG LSRFLSRSR 
      SSLNEEKPK NATRSSSSG PKNAFTSHL KVQSSSLTT 
      GVIYLPKEY RPEQYLENL EKKHAFFAK TFHLEEKRV 
      KCVEEKRLE EHANDECLV QNAFTNLIF SENFEKRLQ 
      QQDNLNFHR SRVQKNENQ IICNYSDII SARRIRELQ 
      ILGCLIVEI FMSKQLRAL GANDSNLSF TDRLKACLT 
      VIKSSEIPP CISYVVHLL LQPDTPNFK EFKYPSVTD 
      LGLPPPSAH LLLEPLLHS VIPFPRLFP YLYSLIATL 
      KDFKNVSTE LGVLYHFDC NGEMCAEYE SLERTKVLF 
      AQNIGECKV KSCARNLES LLEELNTTT DLEVANILL 
      PHVRELIED PPTSVLAAW YLFDPLARV LGPQKTAGS 
      LLEPILKLY ENEPTEATL PYYGKIAKL YHHSFLLRL 
      IVRLGLKCF LDNLVAPLV EAVGRYKDY EKVDFILHT 
      HSEKFVRKT SHLKTMDAE HVDTSGSDD SSVSSEKRP 
      TPRQELASE PEVFDFDDD KQNEEQLKS LMEHLELNV 
      ASDLPFNHS TAEEALDAT LTENLDQLR SLEELTMNL 
      SEDDVETRP GVSSPTIPI PSTKQEFSN ISCEVGSKK 
      SDSIQLDLV LDSPGSAVS DQKKPTVSK SKKQDAKIS 
      DMSSDSLVW LSHRLGPVL TARYLSRNL LKMLTLCYV 
      GKENLTLVQ KEENSREVE TLSVVSSYV LGDQNAVKV 
      LECLTSIAG LYGEQLILF QYIPHMSEL IALCKRRLT 
      ANLEGGLIS CLALFQHVI PYLSDSTLM DQLQDVILK 
      NILHPIVRL LASTKYTFP SGYTARNIL ARKYLDTLY 
      VLSIRMGSD MTRTHLAVP ALQRFFLIF DKVFSGEES 
      HQPEDSQVS QSEKEESHY VELCRDGSV NEWSVGGKP 
      IQISRMNDT DSGLLLEDA SEQSLEELR LVFTTELAY 
      TAYLPFLRH IGANSLEIS LKNHSLIKE LCQEYEQGA 
      KSSVPIANK SADFKCTIS ASSSIGSNI ALVGNRIDV 
      QTADLENFN TDLLSLVSN RLENNSRHL RGNWLAYWE 
      HEIGRSDKD TMFNFKQIK LQTFAGHTH SVKCLYVLD 
      NENSFISGS RDKTVKLWS LRSQGDGSS VSTCQWTYT 
      AHKKSVLSL TFIESMRLV ASCDSVVHI WDPFMGANV 
      GHLESPRYP PVNVLRSLP APSSLVFAA TTDGTVKVI 
      DTRVCNYIH ELKVSLSPT GLIRCLAVA PSGAWVATG 
      QSSGTITVL DTRTGLVIS TWKAHESEV LQLVAADNS 
      TLISSSLDQ TIGVWNASD GKFKFHLRG ATEPVHCLN 
      VYNNELISG TTANRIGVH TSIDMEASF SSTKLRSDA 
      FRGLLTSMA LLPLNRLLL LGADTGTIN LLC
    CDS sequence
    • >TC006498 RA
      atggacatt ttcgaagaa ctgggtgta ccaaagaag 
      tacctgaag cccactact aaagagggg cactacata 
      gctctagta cacaaatca tggctcaag tcattggta 
      aagtactca aaactggtg gagttcgct gaaaggggg 
      cgtttcgac gcatggcca gtcagtgaa gaggagctg 
      ggggcctcg tggagcaaa gtgtttatc tgtgttttc 
      aaaaaacgc gacgctagc gtgatccca ctacctcgc 
      atccggagt ctgattaaa gacgacccc cctttgctc 
      ttctgccag ttgatgcaa tatatttac cagacgaat 
      tataagaat ttgtggaag gaggcttac aagaagtac 
      aacagtaaa actgagact agcaaggag actcaaatt 
      tccctagtg gagtataac gaggttttg cgggagatt 
      ttaacacgg atttacggg tgccccctt gttaatatt 
      tgcgactcg agactgagc accgagcag tcgaaaaac 
      ttcgacgta catttgaac gtgttacca gccgtgtgt 
      gctgtggag actctcaac gcaattttc gtcctacat 
      atgccgtat ttcgaccac agtttgaaa gactgtgtt 
      accttcagc cccgctatt ttaaacaaa tgttacgcc 
      aagccgtta tttataatc taccagttg ttacaaact 
      ctgaagtca atgcacgac cgcagcctc actctgggg 
      gacatctcc ttgagcgac atttaccta accgaagac 
      atgtggatt tacataatc ccgaacatc cactcaaac 
      atttacgtc caagaaacg aaaaccgag gcaaaaaga 
      cacgtaaat atccccgac tgccgcaaa aacggccac 
      aaattcgac ctaaacctt aaatgcgaa ggctgtggc 
      ataaaaacc tacgacaag gtccagatc agcaatgaa 
      accctgcaa gaattgtgc cagttgtgg gtcgaaggc 
      cagatttcg aacttcacc tacatttcc gcgttaaat 
      aaactctcg ggacggaag ttgggtgac cccaactgc 
      caccatgtc tttccgtgg gtgaccgat ttcgcctca 
      aggtgcggc aaaaactgg cgcgatttg aagaaatcc 
      aagtttagg cttaataaa ggggaccgt cagttggac 
      ctgacttac gaaaatgcc tcgtcggag gtgccccac 
      cacgtgtcg gatgtcttg tcttcgatc acgtattac 
      gtgtatatg gctaggagg acccccaag tcggttttg 
      tgcaagaat gtgcggacc atttgggtg ccagcggag 
      taccccagc tccattcag aggatgcag gagtggact 
      ccggacgag tgtattcct gagtttttc accgatccg 
      acggttttt aagagcatt catgacgat ttggacgac 
      ctggaggtc ccctcatgg tgcacgggg cccgaggat 
      tttgtagag aagcacaga gaggccttg gagagtgtt 
      cacgtgtcg gagaggctg cattattgg atagacctg 
      acttttgga tacaaactg gcgggcgcc gccgccgtc 
      aaggccaag aacgtgtgc ctccacctg gtcgacgac 
      cacaccaag ctgatgaaa tcgggaatc gtccaactc 
      ttcacccac ccacacccc ccaaaagcc accccatcc 
      cccttctgg aacaaaacc cccccgaaa atctacatc 
      agcaaaaca aaacccaaa tcccgcgac cgtagcaac 
      tcgcccggt ctcaccctc gacgcgaaa accgacgac 
      gaagacatc gaagacatc ccagcgtcc tcaaaccgc 
      cccctcggc ctctcccgg tttctctcc cgcagccgg 
      tcgtccctc aacgaagaa aaaccgaaa aacgcaacc 
      cggagctcg agctccggg ccgaaaaac gccttcacc 
      tcccacctc aaagtgcaa agttcctcc ctcaccacc 
      ggggtgatt tacttgccg aaggagtac cggccggag 
      caatacttg gagaatttg gagaagaaa catgcgttt 
      ttcgccaag acttttcac cttgaggag aagcgggtg 
      aaatgtgtg gaggagaaa cgactggag gagcacgca 
      aatgacgag tgtttggta caaaatgcg ttcacgaat 
      ttgattttt agcgagaat tttgaaaaa cggttgcaa 
      caacaagac aatctgaat tttcaccgg tcgcgggta 
      caaaagaac gagaatcaa attatttgc aactactct 
      gatatcatc tcagcgagg cgaattcgg gaattgcaa 
      attttgggt tgtttgatt gtggagatt ttcatgtcg 
      aaacagttg cgggctttg ggggccaac gactcgaac 
      ttatcattc accgatcgg ctcaaagcc tgtctcact 
      gttatcaaa agttccgaa atccccccg tgtatctcc 
      tacgttgtt catttgctc ctccagcct gacactccc 
      aacttcaag gagtttaaa tatccgtcc gtgaccgac 
      ttggggttg cctccaccc agcgcccac ctcctcctg 
      gagcccctc ctccactcc gtcatcccg ttcccccga 
      cttttcccc tacttgtat tctctaatc gcgactctt 
      aaagacttc aaaaacgtc tccacggag ttgggagtt 
      ttgtaccac ttcgactgc aacggcgaa atgtgcgca 
      gaatacgaa agtttggaa cggactaaa gtattgttc 
      gcacaaaac atcggcgag tgcaaagtt aaatcatgc 
      gcccgcaac ttggagagt ttgctggag gagttgaac 
      acaacgact gacctagaa gtcgcaaat atcctcctc 
      ccacacgtg cgcgaacta atcgaggat cccccaact 
      tcagtccta gccgcgtgg tatttattc gacccgctt 
      gcccgggtc ttggggccc cagaaaacg gccgggagc 
      ctcctggag cccatttta aaactgtac gaaaatgag 
      ccaaccgag gccactttg ccatactac ggcaaaata 
      gccaagttg taccatcac agttttctc ttacgattg 
      attgtgaga ctggggttg aagtgtttt ttggacaat 
      cttgtggcc ccgcttgtg gaggccgtt gggcggtat 
      aaagactac gaaaaggtc gattttatc ttgcacact 
      cattcggag aagttcgtc cggaaaacg tcgcacttg 
      aagacgatg gacgccgaa catgttgac acttccggg 
      agtgatgac tcgagtgtt tcgtccgaa aaacggcca 
      actccgcga caggagctc gcctccgaa ccggaagtc 
      tttgatttt gacgacgac aaacaaaac gaggagcag 
      ttaaaatcg ctgatggaa catttagag ttaaatgtc 
      gcctcagat ttgcccttc aatcattcg acggcggag 
      gaggcgctc gatgccacc ctgaccgaa aatctggac 
      cagctgagg agtctcgaa gagctcacc atgaacctg 
      agtgaggac gatgttgag accagacct ggggtttcc 
      tccccaact ataccgatc ccgtccacc aagcaagag 
      ttcagtaat attagttgc gaggttggg agcaagaaa 
      agcgattcg attcagctt gatctggtt ttggattcg 
      cccggatcg gcggtttcc gatcaaaag aaaccaact 
      gtctcaaaa tcgaagaaa caagacgcg aaaatttcg 
      gatatgagt tctgatagt ctcgtttgg ttgtcacac 
      agactgggg ccggtgctc acagctcgg tatctttca 
      cggaatttg ctcaaaatg ttgactttg tgttatgtg 
      ggcaaggag aatttgact ctggtgcaa aaagaggag 
      aattcaagg gaggtggag acgctttcg gtggtttcc 
      agctatgtg ctgggggac cagaatgcg gtcaaggtt 
      ttggagtgt ctgacgagc attgcgggt ttatatggc 
      gagcagttg attctgttc caatacatc ccgcacatg 
      agcgaactg atcgctttg tgcaaacga cgcctcacc 
      gccaacttg gagggtggt ttgatctca tgcttggct 
      ttattccaa cacgtcatt ccatactta tctgactcg 
      actctcatg gaccagtta caggacgtg attttaaaa 
      aacatactc catccaatc gtgagactg ctagcttcg 
      acgaaatac acattcccg agcggttac acggctcgt 
      aacatcttg gctcgcaaa tatttggac acactgtac 
      gtcctttcg atccggatg ggctctgat atgacgcga 
      acccacttg gctgtgccc gcccttcag cggtttttt 
      ctcattttt gataaagtg ttctctggg gaggaatcc 
      caccaaccg gaggacagt caagtcagc cagtcggaa 
      aaggaggaa tctcattac gttgagctg tgtcgggac 
      ggatcggtt aacgagtgg agtgtggga gggaaaccg 
      attcaaata tcgcgaatg aacgacacg gactcgggg 
      ttgctgctg gaggacgcc tccgagcag agcctggag 
      gagttacgg ctggttttc accaccgag ttggcctat 
      acggcctat ttgccgttt ttgagacac attggggca 
      aattcgttg gagatttcg ctcaagaat cacagtttg 
      atcaaagag ttgtgccag gagtacgag cagggggcc 
      aaatccagc gtccccatt gcgaataaa tccgccgat 
      tttaaatgc acgatttcg gcttcgagc agcattggg 
      agcaacata gctctggtt gggaaccga atcgacgtc 
      caaaccgcc gatttggaa aacttcaac acggatttg 
      ttgagtctt gtcagcaac cgattggag aacaacagc 
      cggcatttg aggggcaac tggttggcc tattgggag 
      cacgaaatc ggccgctcg gacaaagac acgatgttc 
      aatttcaaa caaatcaaa ctgcaaact ttcgcggga 
      cacacgcac agtgtcaag tgtctctac gtgctagat 
      aacgaaaat agcttcatt tctgggagt cgggacaag 
      acggtcaaa ttgtggtcg ctgaggagt caaggcgac 
      ggctcaagt gtctcaacg tgccagtgg acgtacaca 
      gcccacaaa aagtcggtc ctttcgctc actttcatc 
      gaaagtatg agactagtg gcgtcgtgc gatagcgtc 
      gtgcacatt tgggacccc ttcatgggg gccaacgtg 
      gggcacttg gagtccccc aggtacccc cctgttaac 
      gttttgagg agtttgccg gcgccgtcc agtctcgtg 
      ttcgccgca actaccgac ggaacagtc aaagtgatt 
      gataccagg gtttgcaac tacatccac gaactcaag 
      gtgagtctg agcccgact ggcctgatc cggtgcctt 
      gccgtggcc ccttcgggg gcttgggtg gccacgggc 
      cagtcttcg ggcaccatc accgtcctg gacactcgc 
      accggcttg gtcatttcg acttggaaa gcccacgag 
      agtgaggtc ctccaactc gttgccgcg gacaattca 
      acgctgatt tcgtccagt ttggaccaa acaatcggt 
      gtgtggaac gccagcgat ggcaaattc aagtttcac 
      ctgaggggg gccaccgag ccggtgcac tgcctcaac 
      gtctacaat aacgaactg atttcgggg acaaccgcc 
      aacaggatc ggcgtccac acgtccatc gacatggag 
      gcgtcgttc tccagcacc aagttgcgc tcggacgcg 
      ttcagggga ctgctcacc tccatggcg ctgctgccg 
      ctgaatcgg cttttgctg ttgggggct gacacgggg 
      acgattaat ttgttgtgt tga
    mRNA sequence
    • >TC006498 RA
      tgactcaca aagtgcggt tatgttcaa tcaaaatcc 
      gactttgtt ttgtataca aagatgatc tcagcccgt 
      taaacccca ataatgccc ttttttatc cttttgacg 
      tccccgttc gttcgaaag tgtcgccag atcgcggtt 
      gcttgacac cacatttcc ccgaaatgg acattttcg 
      aagaactgg gtgtaccaa agaagtacc tgaagccca 
      ctactaaag aggggcact acatagctc tagtacaca 
      aatcatggc tcaagtcat tggtaaagt actcaaaac 
      tggtggagt tcgctgaaa gggggcgtt tcgacgcat 
      ggccagtca gtgaagagg agctggggg cctcgtgga 
      gcaaagtgt ttatctgtg ttttcaaaa aacgcgacg 
      ctagcgtga tcccactac ctcgcatcc ggagtctga 
      ttaaagacg acccccctt tgctcttct gccagttga 
      tgcaatata tttaccaga cgaattata agaatttgt 
      ggaaggagg cttacaaga agtacaaca gtaaaactg 
      agactagca aggagactc aaatttccc tagtggagt 
      ataacgagg ttttgcggg agattttaa cacggattt 
      acgggtgcc cccttgtta atatttgcg actcgagac 
      tgagcaccg agcagtcga aaaacttcg acgtacatt 
      tgaacgtgt taccagccg tgtgtgctg tggagactc 
      tcaacgcaa ttttcgtcc tacatatgc cgtatttcg 
      accacagtt tgaaagact gtgttacct tcagccccg 
      ctattttaa acaaatgtt acgccaagc cgttattta 
      taatctacc agttgttac aaactctga agtcaatgc 
      acgaccgca gcctcactc tgggggaca tctccttga 
      gcgacattt acctaaccg aagacatgt ggatttaca 
      taatcccga acatccact caaacattt acgtccaag 
      aaacgaaaa ccgaggcaa aaagacacg taaatatcc 
      ccgactgcc gcaaaaacg gccacaaat tcgacctaa 
      accttaaat gcgaaggct gtggcataa aaacctacg 
      acaaggtcc agatcagca atgaaaccc tgcaagaat 
      tgtgccagt tgtgggtcg aaggccaga tttcgaact 
      tcacctaca tttccgcgt taaataaac tctcgggac 
      ggaagttgg gtgacccca actgccacc atgtctttc 
      cgtgggtga ccgatttcg cctcaaggt gcggcaaaa 
      actggcgcg atttgaaga aatccaagt ttaggctta 
      ataaagggg accgtcagt tggacctga cttacgaaa 
      atgcctcgt cggaggtgc cccaccacg tgtcggatg 
      tcttgtctt cgatcacgt attacgtgt atatggcta 
      ggaggaccc ccaagtcgg ttttgtgca agaatgtgc 
      ggaccattt gggtgccag cggagtacc ccagctcca 
      ttcagagga tgcaggagt ggactccgg acgagtgta 
      ttcctgagt ttttcaccg atccgacgg tttttaaga 
      gcattcatg acgatttgg acgacctgg aggtcccct 
      catggtgca cggggcccg aggattttg tagagaagc 
      acagagagg ccttggaga gtgttcacg tgtcggaga 
      ggctgcatt attggatag acctgactt ttggataca 
      aactggcgg gcgccgccg ccgtcaagg ccaagaacg 
      tgtgcctcc acctggtcg acgaccaca ccaagctga 
      tgaaatcgg gaatcgtcc aactcttca cccacccac 
      accccccaa aagccaccc catccccct tctggaaca 
      aaacccccc cgaaaatct acatcagca aaacaaaac 
      ccaaatccc gcgaccgta gcaactcgc ccggtctca 
      ccctcgacg cgaaaaccg acgacgaag acatcgaag 
      acatcccag cgtcctcaa accgccccc tcggcctct 
      cccggtttc tctcccgca gccggtcgt ccctcaacg 
      aagaaaaac cgaaaaacg caacccgga gctcgagct 
      ccgggccga aaaacgcct tcacctccc acctcaaag 
      tgcaaagtt cctccctca ccaccgggg tgatttact 
      tgccgaagg agtaccggc cggagcaat acttggaga 
      atttggaga agaaacatg cgtttttcg ccaagactt 
      ttcaccttg aggagaagc gggtgaaat gtgtggagg 
      agaaacgac tggaggagc acgcaaatg acgagtgtt 
      tggtacaaa atgcgttca cgaatttga tttttagcg 
      agaattttg aaaaacggt tgcaacaac aagacaatc 
      tgaattttc accggtcgc gggtacaaa agaacgaga 
      atcaaatta tttgcaact actctgata tcatctcag 
      cgaggcgaa ttcgggaat tgcaaattt tgggttgtt 
      tgattgtgg agattttca tgtcgaaac agttgcggg 
      ctttggggg ccaacgact cgaacttat cattcaccg 
      atcggctca aagcctgtc tcactgtta tcaaaagtt 
      ccgaaatcc ccccgtgta tctcctacg ttgttcatt 
      tgctcctcc agcctgaca ctcccaact tcaaggagt 
      ttaaatatc cgtccgtga ccgacttgg ggttgcctc 
      cacccagcg cccacctcc tcctggagc ccctcctcc 
      actccgtca tcccgttcc cccgacttt tcccctact 
      tgtattctc taatcgcga ctcttaaag acttcaaaa 
      acgtctcca cggagttgg gagttttgt accacttcg 
      actgcaacg gcgaaatgt gcgcagaat acgaaagtt 
      tggaacgga ctaaagtat tgttcgcac aaaacatcg 
      gcgagtgca aagttaaat catgcgccc gcaacttgg 
      agagtttgc tggaggagt tgaacacaa cgactgacc 
      tagaagtcg caaatatcc tcctcccac acgtgcgcg 
      aactaatcg aggatcccc caacttcag tcctagccg 
      cgtggtatt tattcgacc cgcttgccc gggtcttgg 
      ggccccaga aaacggccg ggagcctcc tggagccca 
      ttttaaaac tgtacgaaa atgagccaa ccgaggcca 
      ctttgccat actacggca aaatagcca agttgtacc 
      atcacagtt ttctcttac gattgattg tgagactgg 
      ggttgaagt gttttttgg acaatcttg tggccccgc 
      ttgtggagg ccgttgggc ggtataaag actacgaaa 
      aggtcgatt ttatcttgc acactcatt cggagaagt 
      tcgtccgga aaacgtcgc acttgaaga cgatggacg 
      ccgaacatg ttgacactt ccgggagtg atgactcga 
      gtgtttcgt ccgaaaaac ggccaactc cgcgacagg 
      agctcgcct ccgaaccgg aagtctttg attttgacg 
      acgacaaac aaaacgagg agcagttaa aatcgctga 
      tggaacatt tagagttaa atgtcgcct cagatttgc 
      ccttcaatc attcgacgg cggaggagg cgctcgatg 
      ccaccctga ccgaaaatc tggaccagc tgaggagtc 
      tcgaagagc tcaccatga acctgagtg aggacgatg 
      ttgagacca gacctgggg tttcctccc caactatac 
      cgatcccgt ccaccaagc aagagttca gtaatatta 
      gttgcgagg ttgggagca agaaaagcg attcgattc 
      agcttgatc tggttttgg attcgcccg gatcggcgg 
      tttccgatc aaaagaaac caactgtct caaaatcga 
      agaaacaag acgcgaaaa tttcggata tgagttctg 
      atagtctcg tttggttgt cacacagac tggggccgg 
      tgctcacag ctcggtatc tttcacgga atttgctca 
      aaatgttga ctttgtgtt atgtgggca aggagaatt 
      tgactctgg tgcaaaaag aggagaatt caagggagg 
      tggagacgc tttcggtgg tttccagct atgtgctgg 
      gggaccaga atgcggtca aggttttgg agtgtctga 
      cgagcattg cgggtttat atggcgagc agttgattc 
      tgttccaat acatcccgc acatgagcg aactgatcg 
      ctttgtgca aacgacgcc tcaccgcca acttggagg 
      gtggtttga tctcatgct tggctttat tccaacacg 
      tcattccat acttatctg actcgactc tcatggacc 
      agttacagg acgtgattt taaaaaaca tactccatc 
      caatcgtga gactgctag cttcgacga aatacacat 
      tcccgagcg gttacacgg ctcgtaaca tcttggctc 
      gcaaatatt tggacacac tgtacgtcc tttcgatcc 
      ggatgggct ctgatatga cgcgaaccc acttggctg 
      tgcccgccc ttcagcggt tttttctca tttttgata 
      aagtgttct ctggggagg aatcccacc aaccggagg 
      acagtcaag tcagccagt cggaaaagg aggaatctc 
      attacgttg agctgtgtc gggacggat cggttaacg 
      agtggagtg tgggaggga aaccgattc aaatatcgc 
      gaatgaacg acacggact cggggttgc tgctggagg 
      acgcctccg agcagagcc tggaggagt tacggctgg 
      ttttcacca ccgagttgg cctatacgg cctatttgc 
      cgtttttga gacacattg gggcaaatt cgttggaga 
      tttcgctca agaatcaca gtttgatca aagagttgt 
      gccaggagt acgagcagg gggccaaat ccagcgtcc 
      ccattgcga ataaatccg ccgatttta aatgcacga 
      tttcggctt cgagcagca ttgggagca acatagctc 
      tggttggga accgaatcg acgtccaaa ccgccgatt 
      tggaaaact tcaacacgg atttgttga gtcttgtca 
      gcaaccgat tggagaaca acagccggc atttgaggg 
      gcaactggt tggcctatt gggagcacg aaatcggcc 
      gctcggaca aagacacga tgttcaatt tcaaacaaa 
      tcaaactgc aaactttcg cgggacaca cgcacagtg 
      tcaagtgtc tctacgtgc tagataacg aaaatagct 
      tcatttctg ggagtcggg acaagacgg tcaaattgt 
      ggtcgctga ggagtcaag gcgacggct caagtgtct 
      caacgtgcc agtggacgt acacagccc acaaaaagt 
      cggtccttt cgctcactt tcatcgaaa gtatgagac 
      tagtggcgt cgtgcgata gcgtcgtgc acatttggg 
      accccttca tgggggcca acgtggggc acttggagt 
      cccccaggt acccccctg ttaacgttt tgaggagtt 
      tgccggcgc cgtccagtc tcgtgttcg ccgcaacta 
      ccgacggaa cagtcaaag tgattgata ccagggttt 
      gcaactaca tccacgaac tcaaggtga gtctgagcc 
      cgactggcc tgatccggt gccttgccg tggcccctt 
      cgggggctt gggtggcca cgggccagt cttcgggca 
      ccatcaccg tcctggaca ctcgcaccg gcttggtca 
      tttcgactt ggaaagccc acgagagtg aggtcctcc 
      aactcgttg ccgcggaca attcaacgc tgatttcgt 
      ccagtttgg accaaacaa tcggtgtgt ggaacgcca 
      gcgatggca aattcaagt ttcacctga ggggggcca 
      ccgagccgg tgcactgcc tcaacgtct acaataacg 
      aactgattt cggggacaa ccgccaaca ggatcggcg 
      tccacacgt ccatcgaca tggaggcgt cgttctcca 
      gcaccaagt tgcgctcgg acgcgttca ggggactgc 
      tcacctcca tggcgctgc tgccgctga atcggcttt 
      tgctgttgg gggctgaca cggggacga ttaatttgt 
      tgtgttgaa gtcttgccg ggtaagtaa cgtttctca 
      cattccagt gcaattccg taaaaccga tgtgatgtt 
      ttgttttat ttgtatacc gagactgtc agggactat 
      atttacagt tgaacttgc aaaacttta ttttgtaca 
      gatgaataa tagttagcc aatcggaaa ccatcacgg 
      gtgatgggc tgttgttac ctgaaggct agtgttttg 
      ttcaaagtc ccgaaaaat caaaaataa ttgtggcga 
      tttgtagtt cgtgtcact agtcaattt tgtcatcgg 
      ggtcgccac ttattttta cagacctgt accaccatt 
      attttatct ctatttacc tgttcttta gtattatac 
      gatccaaag atatgcaat gcgtgatgt atacaataa 
      gttgtgcaa ataaaattt gtaaagaga g
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