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Gene information
General information
TC006495 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10593413..10595677
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006495
    iBeetle screen iB_04156

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006495 RA
      MWLVGDLTP PLSVPTVLS TFIVCVISF YLFKWLSGD 
      GNVPIRDSS KNHNWTPIK ITAKAWYCS ICESFLING 
      IGVYCDCCG VCADPECVK KANAKLQCK VVTSNNENQ 
      AHQWVKGNL PLGAVCCLC NEDCSLEPG LIDYQCCWC 
      QKTVHTDCL PKLPQECDF GPFKNMIVP PWCVQVARR 
      KGALNKHLL LRGVKDPGW KNWTPLVVI ANKKSGNSD 
      GALLLSEFR KYLNPVQVI DLSARKPPA ALQWAVLLA 
      PREIRVLVG GGDGTVAWV LTTSHKLDL DPEPPLAIV 
      PLGTGNDLS RVLGWGRAN PSELDVGEV LQNVQNSRV 
      AQLDRWRVE ITSSRHLGL RIPNKTLYM YNYIGIGVD 
      AQVALNFHK TRDSLFYVF GSRIFNKIL YLCFGTQQV 
      VTSDCKNLE QKIDLYLDG KQVDLPELE SIVILNIPS 
      WGAGVNLWG KIASI
    • >TC006495 RB
      MWLVGDLTP PLSVPTVLS TFIVCVISF YLFKWLSGD 
      GNVPIRDSS KNHNWTPIK ITAKAWYCS ICESFLING 
      IGVYCDCCG VCADPECVK KANAKLQCK VVTSNNENQ 
      AHQWVKGNL PLGAVCCLC NEDCSLEPG LIDYQCCWC 
      QKTVHTDCL PKLPQECDF GPFKNMIVP PWCVQVARR 
      KGALNKHLL LRGVKDPGW KNWTPLVVI ANKKSGNSD 
      GALLLSEFR KYLNPVQVI DLSARKPPA ALQWAVLLA 
      PREIRVLVG GGDGTVAWV LTTSHKLDL DPEPPLAIV 
      PLGTGNDLS RVLGWGRAN PSELDVGEV LQNVQNSRV 
      AQLDRWRVE ITSSRHLGL RIPNKTLYM YNYIGIGVD 
      AQVALNFHK TRDSLFYVF GSRIFNKIL YLCFGTQQV 
      VTSDCKNLE QKIDLYLDG KQVDLPELE SIVILNIPS 
      WGAGVNLWG LLGNNTKPA QSYHDGILE VLGIYSSFH 
      IAQLQVGLS TPLKLGQAK TVEVRLKSK SPVQIDGEP 
      WEQHPCVMK VTLVNKCPV LINPSRS
    CDS sequence
    • >TC006495 RA
      atgtggcta gtgggtgac ttgacgccc cctctgagc 
      gtccccaca gtgcttagc acttttatc gtttgcgtc 
      ataagtttt taccttttt aaatggttg agtggcgat 
      ggtaatgtc cccattcga gactcctcc aagaatcac 
      aattggacc cccattaaa atcaccgca aaggcgtgg 
      tattgttca atttgtgag tcattttta atcaacggg 
      attggggtt tattgtgac tgttgtggt gtctgtgcc 
      gacccagaa tgcgttaaa aaggccaac gcgaaattg 
      cagtgcaag gtcgtgacc agtaataat gaaaatcag 
      gcgcaccag tgggttaag ggtaattta cccctgggg 
      gctgtgtgt tgcttgtgc aacgaggac tgttcactg 
      gagcccggt ttgattgac taccagtgc tgctggtgt 
      caaaaaacc gtccacact gactgtctt cccaaactc 
      ccccaagag tgtgacttt ggccccttt aaaaacatg 
      atagtcccg ccctggtgc gtgcaagtg gcacgtcgc 
      aaaggcgcc ctcaacaag cacctccta ctccggggc 
      gtcaaagac cccggatgg aaaaactgg acgcctttg 
      gtcgtaatt gcgaataaa aaatcaggc aatagtgat 
      ggggccttg ctattgtct gaattccgg aaatacctg 
      aatccggtc caggtcatc gatttgagt gcccggaag 
      ccgccagcg gccctgcaa tgggccgtg cttttggcc 
      cctagggaa ataagggtg ttggtaggg ggaggtgat 
      gggacagtg gcctgggtc ttgaccacg tcacataag 
      ctagattta gacccggag ccgcctctt gcgattgtg 
      cctctaggg accggaaat gacttaagt agggttctg 
      gggtggggg agggccaat ccgtctgag ttagatgtg 
      ggggaggtg ttgcaaaac gtgcaaaac tcacgagtg 
      gcgcagttg gaccggtgg agagttgaa ataacgtcg 
      tcaagacac ttggggctc cgaattccc aacaaaacg 
      ctctacatg tataattat atcgggatc ggggttgat 
      gcccaggtg gctttgaat ttccacaag acccgagat 
      agcctattc tacgtgttt gggagtaga atttttaat 
      aaaatactt tatttatgt ttcgggacg caacaagtg 
      gttacgtca gattgtaaa aacttggag caaaaaatc 
      gacttgtat ttggacggg aaacaagtc gatttgccc 
      gaactggag tcgattgtt attttaaac atcccatca 
      tggggggcg ggggtcaat ttatggggt aaaatcgca 
      tcaatttag
    • >TC006495 RB
      atgtggcta gtgggtgac ttgacgccc cctctgagc 
      gtccccaca gtgcttagc acttttatc gtttgcgtc 
      ataagtttt taccttttt aaatggttg agtggcgat 
      ggtaatgtc cccattcga gactcctcc aagaatcac 
      aattggacc cccattaaa atcaccgca aaggcgtgg 
      tattgttca atttgtgag tcattttta atcaacggg 
      attggggtt tattgtgac tgttgtggt gtctgtgcc 
      gacccagaa tgcgttaaa aaggccaac gcgaaattg 
      cagtgcaag gtcgtgacc agtaataat gaaaatcag 
      gcgcaccag tgggttaag ggtaattta cccctgggg 
      gctgtgtgt tgcttgtgc aacgaggac tgttcactg 
      gagcccggt ttgattgac taccagtgc tgctggtgt 
      caaaaaacc gtccacact gactgtctt cccaaactc 
      ccccaagag tgtgacttt ggccccttt aaaaacatg 
      atagtcccg ccctggtgc gtgcaagtg gcacgtcgc 
      aaaggcgcc ctcaacaag cacctccta ctccggggc 
      gtcaaagac cccggatgg aaaaactgg acgcctttg 
      gtcgtaatt gcgaataaa aaatcaggc aatagtgat 
      ggggccttg ctattgtct gaattccgg aaatacctg 
      aatccggtc caggtcatc gatttgagt gcccggaag 
      ccgccagcg gccctgcaa tgggccgtg cttttggcc 
      cctagggaa ataagggtg ttggtaggg ggaggtgat 
      gggacagtg gcctgggtc ttgaccacg tcacataag 
      ctagattta gacccggag ccgcctctt gcgattgtg 
      cctctaggg accggaaat gacttaagt agggttctg 
      gggtggggg agggccaat ccgtctgag ttagatgtg 
      ggggaggtg ttgcaaaac gtgcaaaac tcacgagtg 
      gcgcagttg gaccggtgg agagttgaa ataacgtcg 
      tcaagacac ttggggctc cgaattccc aacaaaacg 
      ctctacatg tataattat atcgggatc ggggttgat 
      gcccaggtg gctttgaat ttccacaag acccgagat 
      agcctattc tacgtgttt gggagtaga atttttaat 
      aaaatactt tatttatgt ttcgggacg caacaagtg 
      gttacgtca gattgtaaa aacttggag caaaaaatc 
      gacttgtat ttggacggg aaacaagtc gatttgccc 
      gaactggag tcgattgtt attttaaac atcccatca 
      tggggggcg ggggtcaat ttatggggt cttttgggg 
      aataacaca aagccggca cagtcatac cacgatggt 
      attttggaa gttttggga atttactct tcctttcat 
      attgcacaa ctacaagtt ggtctttcc actccacta 
      aaactaggt caagcaaaa acagttgag gtacgactt 
      aagtccaaa tcgcccgtt cagatcgat ggtgagcct 
      tgggagcag cacccttgc gttatgaaa gtgactttg 
      gtcaataaa tgtcctgta cttattaat ccgagtaga 
      agttag
    mRNA sequence
    • >TC006495 RA
      gacaacaag tttttgttt ataattgtt gaaatgttt 
      taattttaa aaataggcg tgaaaaacc actttaata 
      acgtgttta cgagtgaaa ccagctatt aatattccg 
      aggtcacta cagaagcgg taaagttgg taaaaactc 
      ccggtttca gttgtctgt gtaattacc ctgcttgat 
      ccaccccaa gtggcgaag ttgtacaaa tgcagcccc 
      taattggcg tacggttga atcctgtag ttttgtcgt 
      taccaaata gcggccaaa atgtaataa agttatggc 
      agttttcag taagtttaa actaaactt caaaatgtg 
      gctagtggg tgacttgac gccccctct gagcgtccc 
      cacagtgct tagcacttt tatcgtttg cgtcataag 
      tttttacct ttttaaatg gttgagtgg cgatggtaa 
      tgtccccat tcgagactc ctccaagaa tcacaattg 
      gacccccat taaaatcac cgcaaaggc gtggtattg 
      ttcaatttg tgagtcatt tttaatcaa cgggattgg 
      ggtttattg tgactgttg tggtgtctg tgccgaccc 
      agaatgcgt taaaaaggc caacgcgaa attgcagtg 
      caaggtcgt gaccagtaa taatgaaaa tcaggcgca 
      ccagtgggt taagggtaa tttacccct gggggctgt 
      gtgttgctt gtgcaacga ggactgttc actggagcc 
      cggtttgat tgactacca gtgctgctg gtgtcaaaa 
      aaccgtcca cactgactg tcttcccaa actccccca 
      agagtgtga ctttggccc ctttaaaaa catgatagt 
      cccgccctg gtgcgtgca agtggcacg tcgcaaagg 
      cgccctcaa caagcacct cctactccg gggcgtcaa 
      agaccccgg atggaaaaa ctggacgcc tttggtcgt 
      aattgcgaa taaaaaatc aggcaatag tgatggggc 
      cttgctatt gtctgaatt ccggaaata cctgaatcc 
      ggtccaggt catcgattt gagtgcccg gaagccgcc 
      agcggccct gcaatgggc cgtgctttt ggcccctag 
      ggaaataag ggtgttggt agggggagg tgatgggac 
      agtggcctg ggtcttgac cacgtcaca taagctaga 
      tttagaccc ggagccgcc tcttgcgat tgtgcctct 
      agggaccgg aaatgactt aagtagggt tctggggtg 
      ggggagggc caatccgtc tgagttaga tgtggggga 
      ggtgttgca aaacgtgca aaactcacg agtggcgca 
      gttggaccg gtggagagt tgaaataac gtcgtcaag 
      acacttggg gctccgaat tcccaacaa aacgctcta 
      catgtataa ttatatcgg gatcggggt tgatgccca 
      ggtggcttt gaatttcca caagacccg agatagcct 
      attctacgt gtttgggag tagaatttt taataaaat 
      actttattt atgtttcgg gacgcaaca agtggttac 
      gtcagattg taaaaactt ggagcaaaa aatcgactt 
      gtatttgga cgggaaaca agtcgattt gcccgaact 
      ggagtcgat tgttatttt aaacatccc atcatgggg 
      ggcgggggt caatttatg gggtaaaat cgcatcaat 
      ttagtgttt tgtgcccaa agttgtttc aggtctttt 
      ggggaataa cacaaagcc ggcacagtc ataccacga 
      tggtatttt ggaagtttt gggaattta ctcttcctt 
      tcatattgc acaactaca agttggtct ttccactcc 
      actaaaact aggtcaagc aaaaacagt tgaggtacg 
      acttaagtc caaatcgcc cgttcagat cgatggtga 
      gccttggga gcagcaccc ttgcgttat gaaagtgac 
      tttggtcaa taaatgtcc tgtacttat taatccgag 
      tagaagtta gaaacaaat gcatttacc taattaagc 
      atttactcc cagtttctt gccgtgata cttaaggtt 
      tttaacgtt ttt
    • >TC006495 RB
      gacaacaag tttttgttt ataattgtt gaaatgttt 
      taattttaa aaataggcg tgaaaaacc actttaata 
      acgtgttta cgagtgaaa ccagctatt aatattccg 
      aggtcacta cagaagcgg taaagttgg taaaaactc 
      ccggtttca gttgtctgt gtaattacc ctgcttgat 
      ccaccccaa gtggcgaag ttgtacaaa tgcagcccc 
      taattggcg tacggttga atcctgtag ttttgtcgt 
      taccaaata gcggccaaa atgtaataa agttatggc 
      agttttcag taagtttaa actaaactt caaaatgtg 
      gctagtggg tgacttgac gccccctct gagcgtccc 
      cacagtgct tagcacttt tatcgtttg cgtcataag 
      tttttacct ttttaaatg gttgagtgg cgatggtaa 
      tgtccccat tcgagactc ctccaagaa tcacaattg 
      gacccccat taaaatcac cgcaaaggc gtggtattg 
      ttcaatttg tgagtcatt tttaatcaa cgggattgg 
      ggtttattg tgactgttg tggtgtctg tgccgaccc 
      agaatgcgt taaaaaggc caacgcgaa attgcagtg 
      caaggtcgt gaccagtaa taatgaaaa tcaggcgca 
      ccagtgggt taagggtaa tttacccct gggggctgt 
      gtgttgctt gtgcaacga ggactgttc actggagcc 
      cggtttgat tgactacca gtgctgctg gtgtcaaaa 
      aaccgtcca cactgactg tcttcccaa actccccca 
      agagtgtga ctttggccc ctttaaaaa catgatagt 
      cccgccctg gtgcgtgca agtggcacg tcgcaaagg 
      cgccctcaa caagcacct cctactccg gggcgtcaa 
      agaccccgg atggaaaaa ctggacgcc tttggtcgt 
      aattgcgaa taaaaaatc aggcaatag tgatggggc 
      cttgctatt gtctgaatt ccggaaata cctgaatcc 
      ggtccaggt catcgattt gagtgcccg gaagccgcc 
      agcggccct gcaatgggc cgtgctttt ggcccctag 
      ggaaataag ggtgttggt agggggagg tgatgggac 
      agtggcctg ggtcttgac cacgtcaca taagctaga 
      tttagaccc ggagccgcc tcttgcgat tgtgcctct 
      agggaccgg aaatgactt aagtagggt tctggggtg 
      ggggagggc caatccgtc tgagttaga tgtggggga 
      ggtgttgca aaacgtgca aaactcacg agtggcgca 
      gttggaccg gtggagagt tgaaataac gtcgtcaag 
      acacttggg gctccgaat tcccaacaa aacgctcta 
      catgtataa ttatatcgg gatcggggt tgatgccca 
      ggtggcttt gaatttcca caagacccg agatagcct 
      attctacgt gtttgggag tagaatttt taataaaat 
      actttattt atgtttcgg gacgcaaca agtggttac 
      gtcagattg taaaaactt ggagcaaaa aatcgactt 
      gtatttgga cgggaaaca agtcgattt gcccgaact 
      ggagtcgat tgttatttt aaacatccc atcatgggg 
      ggcgggggt caatttatg gggtctttt ggggaataa 
      cacaaagcc ggcacagtc ataccacga tggtatttt 
      ggaagtttt gggaattta ctcttcctt tcatattgc 
      acaactaca agttggtct ttccactcc actaaaact 
      aggtcaagc aaaaacagt tgaggtacg acttaagtc 
      caaatcgcc cgttcagat cgatggtga gccttggga 
      gcagcaccc ttgcgttat gaaagtgac tttggtcaa 
      taaatgtcc tgtacttat taatccgag tagaagtta 
      gaaacaaat gcatttacc taattaagc atttactcc 
      cagtttctt gccgtgata cttaaggtt tttaacgtt 
      ttt
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