Status
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Gene information
General information
TC006494 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10583890..10589236
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006494
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04155

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006494 RA
      MESGDESGL STQEALQRL HAEIEAVLQ QLKQSQKAR 
      KSRSWIKDT FHHSSQDTT INWVSASFQ LWAVASMFL 
      AFIIEQNFV LCVESITLL LFLLINVTL ELYDNKLRH 
      EEVPNRVQA VLDTIKRIS EGRWEKENY PNLCSPFSP 
      CITLQWTYR DNKLVNLPW ALLVKDDLI VIRPGQVSP 
      GYCEAVDKT SEFPLLHAK EVYAPSLQS ANEIFSAPK 
      IRKPLENKK YRLLETPYL NNLRIYLEQ SLDRPCTLL 
      NKQRHLVMI KIIERFVLP ICFIVTFLV NLFRFLFPL 
      GPSQPSVMF LIVPISTIL PLLPLVFPM AWNLLNYFG 
      LARFRCILD ISKQIEAYK DNPLEEDTE TSTIDHQEL 
      KCRRQELWK NFVSIVKGQ PDLLVHSAN ILHVLGSVT 
      ALGCVDKKG ILSWPNPTA EKIFFLHNN ENASPSSSL 
      GNVGDLAPD GDSAGGTPH HYFESPNTA TGEVLDLTH 
      NHSDPFKLH FDDQEWHQY LTSLKPLGL AILLNTCNV 
      ETQEHYAAF CSHITCEAI YNENLVPVS NRRCLCELA 
      KEIGFVDQA QKIFALEQQ LSTFRHLQP EMVRSDNKY 
      ARSLQLSTK LKFPFPHMF AVVVKELSS GSMQLLSEG 
      TADIILDSC VDYWDGHDL CPLTPSDRK KIQDFYQRS 
      SLTAYCTAF AYRPLNKCV SSKLGGVYL ELPSDSRHL 
      YMNYRSPTP VHWDCKSVL EPKIKTPLG QFFSTDSLL 
      YNDCPSVDV SDAESCFQM QCNQVFIGM VTMQYQALT 
      DMVQLIEQL ERACIRFVH FSKENELRS RVFSEKMGL 
      ESGWNCHIS LLSERNSNL ESSRTMSFS APSAINMEH 
      SVVKFDTEQ ERLNKNSSD QSGVYSTKV SHDSVQIHN 
      EINTSHDWQ SLSCLTDST EQSAPINFD MSNRAKLPR 
      GIDKIRPHI ELIDNVPLL VSLFTDCTP SATREMLNI 
      MQDYGEIVC IMGSSANCE NAGIFMQAD ASIAVDPLF 
      PQMCQKVPV YKKPKMGVS PLDLSRDLN SIACSLCLK 
      REDPISFYH LVMEARHFV TILWSSVQF WVSCCVILS 
      VLQLVSVLV MLPPVLTTG QVLWLTCFV VPLLSVSLM 
      GLPVDPEVM KKPQGKNQV GVNLYISMF ILWCYGLKF 
      FPTVVVVIV TFVGILAEH CVEICGVRN CTCQAFYLS 
      TEFMWEEES WTIATARLI IVFIVLLHF VVISIGFVF 
      RDYLIYKRP PIKNLYWIV TCFILVALQ SIYTILSFY 
      NITNNTDNS IQFQIPLWV IIFAAISPF LVLIINEVI 
      KQQEIMVNK RFRKRARLD FGTKLGMNS PF
    CDS sequence
    • >TC006494 RA
      atggagtcg ggcgacgag tcaggtctg agcacccag 
      gaggccctc cagcggctc cacgccgag atcgaggcg 
      gttctgcag cagctgaag cagtcccaa aaggccaga 
      aaatcccgc tcctggatc aaagacacg ttccaccac 
      tccagccaa gacaccacg atcaattgg gtgtctgcg 
      agtttccag ttgtgggcg gtggccagc atgttcctt 
      gcttttata attgaacaa aattttgtc ttgtgtgtt 
      gagagtatc acacttttg ctttttctc ctaattaac 
      gtgacgttg gaactgtac gataataaa ttgaggcat 
      gaggaggtg ccaaaccgg gtgcaggcg gttcttgac 
      acgattaag aggatctcg gaggggcgc tgggaaaag 
      gagaattac cctaacttg tgttcaccg ttttcgccg 
      tgtattacg ctgcagtgg acctacagg gacaacaaa 
      ctagtcaat ctaccgtgg gccctattg gtcaaggat 
      gacctaatt gtgataagg ccgggacaa gtgagcccc 
      ggctattgc gaagccgtt gataaaacc tccgaattt 
      ccgctcctc cacgccaaa gaagtatac gcaccgtcg 
      ctacaaagt gccaacgag attttcagt gccccaaaa 
      atacggaaa ccgctagaa aataaaaaa taccgactg 
      ctggaaacc ccatacttg aacaatttg cggatctac 
      ttggaacag tcactagac aggccttgc accctcctc 
      aacaaacag aggcactta gttatgatt aaaataatc 
      gagcgtttt gtcctccct atttgtttt attgtcacg 
      ttccttgtg aatttgttc cgatttttg ttcccgttg 
      ggccccagc cagccctcg gtgatgttc ctaatcgtc 
      cccatttcc acaatccta cctttgctt ccgttagtt 
      ttccccatg gcctggaac ctgttgaat tacttcggt 
      ctggcgcga tttcgctgc attttggac atttccaaa 
      caaattgaa gcctacaaa gacaacccc ctcgaagag 
      gacaccgag acttcgacc atcgaccac caagagctg 
      aaatgtcgg cggcaggaa ctttggaaa aatttcgtt 
      tcgattgtt aaaggccag ccagatttg ttggtacat 
      tccgccaat attttgcac gtcttgggc tcggtcact 
      gcacttggg tgcgtcgat aaaaaagga atcctctcg 
      tggccaaac cccacggct gaaaaaatc tttttcctc 
      cacaataac gaaaacgct tcgccttcc tctagtctg 
      ggcaacgtg ggggacttg gcccctgac ggggacagc 
      gccggtggc accccacac cactacttc gaaagtcca 
      aacaccgca actggggaa gttctggac ttgacgcac 
      aatcacagc gaccctttt aaactccat ttcgacgac 
      caggagtgg caccaatac ttaacctcc ctgaagccg 
      ctaggcctt gctattttg ttaaacact tgcaacgtt 
      gaaacccag gagcattat gcggcattt tgtagccac 
      attacgtgc gaggccatt tataacgaa aatctggtt 
      ccggtgagc aaccgacgg tgtctgtgc gagttggcc 
      aaagaaatt ggatttgtg gaccaggcc cagaaaatt 
      ttcgcgctt gaacaacaa ctctccacc tttcggcac 
      ttgcagccg gaaatggtc cgaagtgat aataagtat 
      gcgaggtcg cttcaactt tcaacaaaa ctcaaattt 
      ccgttcccg cacatgttt gctgtggtg gtcaaagag 
      ttgagcagt ggctccatg cagttgttg tctgaagga 
      acggctgat ataattttg gattcgtgc gtggattat 
      tgggacggg catgatctg tgccctttg actccttcc 
      gaccggaaa aaaatccag gatttttac cagaggagc 
      agcctcacg gcgtattgt acggcgttc gcttacagg 
      cccttaaat aaatgtgtt agtagtaaa ttaggggga 
      gtttatttg gaattgccg agtgatagt agacacttg 
      tatatgaat taccggagt ccgacgcca gtccattgg 
      gactgcaag agcgtcctt gagccgaaa attaagact 
      cctttgggg caatttttt agcactgac tcgcttttg 
      tacaatgat tgtccgtcg gttgatgtt tccgatgct 
      gagagttgc ttccaaatg cagtgtaat caggtcttt 
      atcggaatg gtgactatg cagtaccag gcattgacc 
      gatatggtg cagctaatc gaacagctg gaacgtgcc 
      tgcatccga ttcgtccat ttcagcaag gaaaacgag 
      ctgcgttcg cgcgttttc agcgagaaa atgggcctg 
      gaaagcggc tggaattgc cacatttcc cttttgagc 
      gaacgcaac agcaatttg gagtcgtcc cgaacgatg 
      agcttctcc gcccccagt gccataaac atggagcat 
      tcggttgtg aagtttgac acggaacag gagcgcctc 
      aacaaaaat tcaagcgat caaagcggc gtttactcc 
      acgaaagtg agccacgac agcgtccag atccacaac 
      gagatcaac acgtcgcac gactggcag tctctgagt 
      tgcctcacg gacagtacc gagcaaagc gccccaatt 
      aacttcgat atgagcaat cgggcgaaa ctaccccgt 
      ggtattgac aaaatccgc ccccatatc gagctaatc 
      gacaacgtc cccttgcta gtgtccctc tttaccgac 
      tgcacgccc agcgcgacc cgcgaaatg ctcaacatc 
      atgcaagac tacggcgaa atcgtgtgc ataatgggc 
      tcctcggcc aactgcgag aacgcgggg attttcatg 
      caggccgat gcgagcatc gcagtggac cccctgttc 
      ccccaaatg tgccagaaa gtcccagtt tacaagaag 
      ccaaaaatg ggggtttca ccgctcgat ctgtcgcgc 
      gacttgaac tcgatcgct tgctccctg tgcctcaaa 
      cgcgaggac ccgatctcg ttctatcac ctagtcatg 
      gaggcgcgg catttcgtc acaatcctc tggagttcg 
      gtccagttt tgggtgtct tgttgcgtt atcttgagt 
      gttctgcag ctggtctcg gtgctggtg atgttgccc 
      cccgttttg acaacgggg caagtcttg tggttgacg 
      tgttttgtt gttcctctg ttgagtgtg tcgctgatg 
      gggcttccg gtggatccg gaggtcatg aagaagccg 
      caagggaag aaccaagtc ggggtcaat ctctatatt 
      tcgatgttt attctgtgg tgttacgga ttgaagttt 
      tttccgact gttgttgtt gttattgtg acgtttgta 
      ggcatcttg gctgagcat tgtgttgag atttgtggc 
      gtgcgtaat tgcacctgt caggcgttt tatctatcg 
      actgagttc atgtgggaa gaagagtcc tggacgatt 
      gcaaccgca agacttatt attgtgttc attgtttta 
      ctgcatttt gttgttatt tcgataggt tttgttttt 
      agagattat ttaatttat aaaagaccg ccaatcaaa 
      aacctttac tggatagtc acttgtttt attctagtg 
      gcgcttcag agtatttat acgatttta agtttttac 
      aacattacg aataatacc gataatagt atccagttt 
      caaatacca ctttgggtg ataattttt gccgcaata 
      tctccgttt ttagtgctg attattaac gaagtgatc 
      aaacagcag gaaataatg gtaaataaa cgattcagg 
      aagcgggcg cgactggac tttgggaca aaattggga 
      atgaattct ccattttag
    mRNA sequence
    • >TC006494 RA
      tcgctcacc ttgaatagt ggccagaac taaacccaa 
      cgtccttga cggcactaa ccaggcacg tgttggact 
      catacaaat tacactttt gtacacttg gattcacgc 
      aactccacc ctcagtgtg attaaatca ccgaatttc 
      ggggataaa aacgcaaaa acaacgcaa catgtgtga 
      taacgcttg acggttaat tgacagttg acagctgtt 
      caaggtttt gtggttata tcaaaaata atgtaacga 
      attcccgaa attcccgtg tgtggtaaa cagttgggc 
      cgtttcgtg ggttctgtg atgacataa tgtgagacg 
      ggatggagt cgggcgacg agtcaggtc tgagcaccc 
      aggaggccc tccagcggc tccacgccg agatcgagg 
      cggttctgc agcagctga agcagtccc aaaaggcca 
      gaaaatccc gctcctgga tcaaagaca cgttccacc 
      actccagcc aagacacca cgatcaatt gggtgtctg 
      cgagtttcc agttgtggg cggtggcca gcatgttcc 
      ttgctttta taattgaac aaaattttg tcttgtgtg 
      ttgagagta tcacacttt tgctttttc tcctaatta 
      acgtgacgt tggaactgt acgataata aattgaggc 
      atgaggagg tgccaaacc gggtgcagg cggttcttg 
      acacgatta agaggatct cggaggggc gctgggaaa 
      aggagaatt accctaact tgtgttcac cgttttcgc 
      cgtgtatta cgctgcagt ggacctaca gggacaaca 
      aactagtca atctaccgt gggccctat tggtcaagg 
      atgacctaa ttgtgataa ggccgggac aagtgagcc 
      ccggctatt gcgaagccg ttgataaaa cctccgaat 
      ttccgctcc tccacgcca aagaagtat acgcaccgt 
      cgctacaaa gtgccaacg agattttca gtgccccaa 
      aaatacgga aaccgctag aaaataaaa aataccgac 
      tgctggaaa ccccatact tgaacaatt tgcggatct 
      acttggaac agtcactag acaggcctt gcaccctcc 
      tcaacaaac agaggcact tagttatga ttaaaataa 
      tcgagcgtt ttgtcctcc ctatttgtt ttattgtca 
      cgttccttg tgaatttgt tccgatttt tgttcccgt 
      tgggcccca gccagccct cggtgatgt tcctaatcg 
      tccccattt ccacaatcc tacctttgc ttccgttag 
      ttttcccca tggcctgga acctgttga attacttcg 
      gtctggcgc gatttcgct gcattttgg acatttcca 
      aacaaattg aagcctaca aagacaacc ccctcgaag 
      aggacaccg agacttcga ccatcgacc accaagagc 
      tgaaatgtc ggcggcagg aactttgga aaaatttcg 
      tttcgattg ttaaaggcc agccagatt tgttggtac 
      attccgcca atattttgc acgtcttgg gctcggtca 
      ctgcacttg ggtgcgtcg ataaaaaag gaatcctct 
      cgtggccaa accccacgg ctgaaaaaa tctttttcc 
      tccacaata acgaaaacg cttcgcctt cctctagtc 
      tgggcaacg tgggggact tggcccctg acggggaca 
      gcgccggtg gcaccccac accactact tcgaaagtc 
      caaacaccg caactgggg aagttctgg acttgacgc 
      acaatcaca gcgaccctt ttaaactcc atttcgacg 
      accaggagt ggcaccaat acttaacct ccctgaagc 
      cgctaggcc ttgctattt tgttaaaca cttgcaacg 
      ttgaaaccc aggagcatt atgcggcat tttgtagcc 
      acattacgt gcgaggcca tttataacg aaaatctgg 
      ttccggtga gcaaccgac ggtgtctgt gcgagttgg 
      ccaaagaaa ttggatttg tggaccagg cccagaaaa 
      ttttcgcgc ttgaacaac aactctcca cctttcggc 
      acttgcagc cggaaatgg tccgaagtg ataataagt 
      atgcgaggt cgcttcaac tttcaacaa aactcaaat 
      ttccgttcc cgcacatgt ttgctgtgg tggtcaaag 
      agttgagca gtggctcca tgcagttgt tgtctgaag 
      gaacggctg atataattt tggattcgt gcgtggatt 
      attgggacg ggcatgatc tgtgccctt tgactcctt 
      ccgaccgga aaaaaatcc aggattttt accagagga 
      gcagcctca cggcgtatt gtacggcgt tcgcttaca 
      ggcccttaa ataaatgtg ttagtagta aattagggg 
      gagtttatt tggaattgc cgagtgata gtagacact 
      tgtatatga attaccgga gtccgacgc cagtccatt 
      gggactgca agagcgtcc ttgagccga aaattaaga 
      ctcctttgg ggcaatttt ttagcactg actcgcttt 
      tgtacaatg attgtccgt cggttgatg tttccgatg 
      ctgagagtt gcttccaaa tgcagtgta atcaggtct 
      ttatcggaa tggtgacta tgcagtacc aggcattga 
      ccgatatgg tgcagctaa tcgaacagc tggaacgtg 
      cctgcatcc gattcgtcc atttcagca aggaaaacg 
      agctgcgtt cgcgcgttt tcagcgaga aaatgggcc 
      tggaaagcg gctggaatt gccacattt cccttttga 
      gcgaacgca acagcaatt tggagtcgt cccgaacga 
      tgagcttct ccgccccca gtgccataa acatggagc 
      attcggttg tgaagtttg acacggaac aggagcgcc 
      tcaacaaaa attcaagcg atcaaagcg gcgtttact 
      ccacgaaag tgagccacg acagcgtcc agatccaca 
      acgagatca acacgtcgc acgactggc agtctctga 
      gttgcctca cggacagta ccgagcaaa gcgccccaa 
      ttaacttcg atatgagca atcgggcga aactacccc 
      gtggtattg acaaaatcc gcccccata tcgagctaa 
      tcgacaacg tccccttgc tagtgtccc tctttaccg 
      actgcacgc ccagcgcga cccgcgaaa tgctcaaca 
      tcatgcaag actacggcg aaatcgtgt gcataatgg 
      gctcctcgg ccaactgcg agaacgcgg ggattttca 
      tgcaggccg atgcgagca tcgcagtgg accccctgt 
      tcccccaaa tgtgccaga aagtcccag tttacaaga 
      agccaaaaa tgggggttt caccgctcg atctgtcgc 
      gcgacttga actcgatcg cttgctccc tgtgcctca 
      aacgcgagg acccgatct cgttctatc acctagtca 
      tggaggcgc ggcatttcg tcacaatcc tctggagtt 
      cggtccagt tttgggtgt cttgttgcg ttatcttga 
      gtgttctgc agctggtct cggtgctgg tgatgttgc 
      cccccgttt tgacaacgg ggcaagtct tgtggttga 
      cgtgttttg ttgttcctc tgttgagtg tgtcgctga 
      tggggcttc cggtggatc cggaggtca tgaagaagc 
      cgcaaggga agaaccaag tcggggtca atctctata 
      tttcgatgt ttattctgt ggtgttacg gattgaagt 
      tttttccga ctgttgttg ttgttattg tgacgtttg 
      taggcatct tggctgagc attgtgttg agatttgtg 
      gcgtgcgta attgcacct gtcaggcgt tttatctat 
      cgactgagt tcatgtggg aagaagagt cctggacga 
      ttgcaaccg caagactta ttattgtgt tcattgttt 
      tactgcatt ttgttgtta tttcgatag gttttgttt 
      ttagagatt atttaattt ataaaagac cgccaatca 
      aaaaccttt actggatag tcacttgtt ttattctag 
      tggcgcttc agagtattt atacgattt taagttttt 
      acaacatta cgaataata ccgataata gtatccagt 
      ttcaaatac cactttggg tgataattt ttgccgcaa 
      tatctccgt ttttagtgc tgattatta acgaagtga 
      tcaaacagc aggaaataa tggtaaata aacgattca 
      ggaagcggg cgcgactgg actttggga caaaattgg 
      gaatgaatt ctccatttt agcgatacg aagtgaaaa 
      ctatatttt tatatatcg gtgtaacgg aaccagttg 
      actgatttc atttgataa gactggaat gtttgtaaa 
      aaataaagt gttgatttt aag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY