| General information | |||
| TC006494 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 10583890..10589236 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006494 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04155 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC006494 RA
MESGDESGL STQEALQRL HAEIEAVLQ QLKQSQKAR KSRSWIKDT FHHSSQDTT INWVSASFQ LWAVASMFL AFIIEQNFV LCVESITLL LFLLINVTL ELYDNKLRH EEVPNRVQA VLDTIKRIS EGRWEKENY PNLCSPFSP CITLQWTYR DNKLVNLPW ALLVKDDLI VIRPGQVSP GYCEAVDKT SEFPLLHAK EVYAPSLQS ANEIFSAPK IRKPLENKK YRLLETPYL NNLRIYLEQ SLDRPCTLL NKQRHLVMI KIIERFVLP ICFIVTFLV NLFRFLFPL GPSQPSVMF LIVPISTIL PLLPLVFPM AWNLLNYFG LARFRCILD ISKQIEAYK DNPLEEDTE TSTIDHQEL KCRRQELWK NFVSIVKGQ PDLLVHSAN ILHVLGSVT ALGCVDKKG ILSWPNPTA EKIFFLHNN ENASPSSSL GNVGDLAPD GDSAGGTPH HYFESPNTA TGEVLDLTH NHSDPFKLH FDDQEWHQY LTSLKPLGL AILLNTCNV ETQEHYAAF CSHITCEAI YNENLVPVS NRRCLCELA KEIGFVDQA QKIFALEQQ LSTFRHLQP EMVRSDNKY ARSLQLSTK LKFPFPHMF AVVVKELSS GSMQLLSEG TADIILDSC VDYWDGHDL CPLTPSDRK KIQDFYQRS SLTAYCTAF AYRPLNKCV SSKLGGVYL ELPSDSRHL YMNYRSPTP VHWDCKSVL EPKIKTPLG QFFSTDSLL YNDCPSVDV SDAESCFQM QCNQVFIGM VTMQYQALT DMVQLIEQL ERACIRFVH FSKENELRS RVFSEKMGL ESGWNCHIS LLSERNSNL ESSRTMSFS APSAINMEH SVVKFDTEQ ERLNKNSSD QSGVYSTKV SHDSVQIHN EINTSHDWQ SLSCLTDST EQSAPINFD MSNRAKLPR GIDKIRPHI ELIDNVPLL VSLFTDCTP SATREMLNI MQDYGEIVC IMGSSANCE NAGIFMQAD ASIAVDPLF PQMCQKVPV YKKPKMGVS PLDLSRDLN SIACSLCLK REDPISFYH LVMEARHFV TILWSSVQF WVSCCVILS VLQLVSVLV MLPPVLTTG QVLWLTCFV VPLLSVSLM GLPVDPEVM KKPQGKNQV GVNLYISMF ILWCYGLKF FPTVVVVIV TFVGILAEH CVEICGVRN CTCQAFYLS TEFMWEEES WTIATARLI IVFIVLLHF VVISIGFVF RDYLIYKRP PIKNLYWIV TCFILVALQ SIYTILSFY NITNNTDNS IQFQIPLWV IIFAAISPF LVLIINEVI KQQEIMVNK RFRKRARLD FGTKLGMNS PF
- >TC006494 RA
atggagtcg ggcgacgag tcaggtctg agcacccag gaggccctc cagcggctc cacgccgag atcgaggcg gttctgcag cagctgaag cagtcccaa aaggccaga aaatcccgc tcctggatc aaagacacg ttccaccac tccagccaa gacaccacg atcaattgg gtgtctgcg agtttccag ttgtgggcg gtggccagc atgttcctt gcttttata attgaacaa aattttgtc ttgtgtgtt gagagtatc acacttttg ctttttctc ctaattaac gtgacgttg gaactgtac gataataaa ttgaggcat gaggaggtg ccaaaccgg gtgcaggcg gttcttgac acgattaag aggatctcg gaggggcgc tgggaaaag gagaattac cctaacttg tgttcaccg ttttcgccg tgtattacg ctgcagtgg acctacagg gacaacaaa ctagtcaat ctaccgtgg gccctattg gtcaaggat gacctaatt gtgataagg ccgggacaa gtgagcccc ggctattgc gaagccgtt gataaaacc tccgaattt ccgctcctc cacgccaaa gaagtatac gcaccgtcg ctacaaagt gccaacgag attttcagt gccccaaaa atacggaaa ccgctagaa aataaaaaa taccgactg ctggaaacc ccatacttg aacaatttg cggatctac ttggaacag tcactagac aggccttgc accctcctc aacaaacag aggcactta gttatgatt aaaataatc gagcgtttt gtcctccct atttgtttt attgtcacg ttccttgtg aatttgttc cgatttttg ttcccgttg ggccccagc cagccctcg gtgatgttc ctaatcgtc cccatttcc acaatccta cctttgctt ccgttagtt ttccccatg gcctggaac ctgttgaat tacttcggt ctggcgcga tttcgctgc attttggac atttccaaa caaattgaa gcctacaaa gacaacccc ctcgaagag gacaccgag acttcgacc atcgaccac caagagctg aaatgtcgg cggcaggaa ctttggaaa aatttcgtt tcgattgtt aaaggccag ccagatttg ttggtacat tccgccaat attttgcac gtcttgggc tcggtcact gcacttggg tgcgtcgat aaaaaagga atcctctcg tggccaaac cccacggct gaaaaaatc tttttcctc cacaataac gaaaacgct tcgccttcc tctagtctg ggcaacgtg ggggacttg gcccctgac ggggacagc gccggtggc accccacac cactacttc gaaagtcca aacaccgca actggggaa gttctggac ttgacgcac aatcacagc gaccctttt aaactccat ttcgacgac caggagtgg caccaatac ttaacctcc ctgaagccg ctaggcctt gctattttg ttaaacact tgcaacgtt gaaacccag gagcattat gcggcattt tgtagccac attacgtgc gaggccatt tataacgaa aatctggtt ccggtgagc aaccgacgg tgtctgtgc gagttggcc aaagaaatt ggatttgtg gaccaggcc cagaaaatt ttcgcgctt gaacaacaa ctctccacc tttcggcac ttgcagccg gaaatggtc cgaagtgat aataagtat gcgaggtcg cttcaactt tcaacaaaa ctcaaattt ccgttcccg cacatgttt gctgtggtg gtcaaagag ttgagcagt ggctccatg cagttgttg tctgaagga acggctgat ataattttg gattcgtgc gtggattat tgggacggg catgatctg tgccctttg actccttcc gaccggaaa aaaatccag gatttttac cagaggagc agcctcacg gcgtattgt acggcgttc gcttacagg cccttaaat aaatgtgtt agtagtaaa ttaggggga gtttatttg gaattgccg agtgatagt agacacttg tatatgaat taccggagt ccgacgcca gtccattgg gactgcaag agcgtcctt gagccgaaa attaagact cctttgggg caatttttt agcactgac tcgcttttg tacaatgat tgtccgtcg gttgatgtt tccgatgct gagagttgc ttccaaatg cagtgtaat caggtcttt atcggaatg gtgactatg cagtaccag gcattgacc gatatggtg cagctaatc gaacagctg gaacgtgcc tgcatccga ttcgtccat ttcagcaag gaaaacgag ctgcgttcg cgcgttttc agcgagaaa atgggcctg gaaagcggc tggaattgc cacatttcc cttttgagc gaacgcaac agcaatttg gagtcgtcc cgaacgatg agcttctcc gcccccagt gccataaac atggagcat tcggttgtg aagtttgac acggaacag gagcgcctc aacaaaaat tcaagcgat caaagcggc gtttactcc acgaaagtg agccacgac agcgtccag atccacaac gagatcaac acgtcgcac gactggcag tctctgagt tgcctcacg gacagtacc gagcaaagc gccccaatt aacttcgat atgagcaat cgggcgaaa ctaccccgt ggtattgac aaaatccgc ccccatatc gagctaatc gacaacgtc cccttgcta gtgtccctc tttaccgac tgcacgccc agcgcgacc cgcgaaatg ctcaacatc atgcaagac tacggcgaa atcgtgtgc ataatgggc tcctcggcc aactgcgag aacgcgggg attttcatg caggccgat gcgagcatc gcagtggac cccctgttc ccccaaatg tgccagaaa gtcccagtt tacaagaag ccaaaaatg ggggtttca ccgctcgat ctgtcgcgc gacttgaac tcgatcgct tgctccctg tgcctcaaa cgcgaggac ccgatctcg ttctatcac ctagtcatg gaggcgcgg catttcgtc acaatcctc tggagttcg gtccagttt tgggtgtct tgttgcgtt atcttgagt gttctgcag ctggtctcg gtgctggtg atgttgccc cccgttttg acaacgggg caagtcttg tggttgacg tgttttgtt gttcctctg ttgagtgtg tcgctgatg gggcttccg gtggatccg gaggtcatg aagaagccg caagggaag aaccaagtc ggggtcaat ctctatatt tcgatgttt attctgtgg tgttacgga ttgaagttt tttccgact gttgttgtt gttattgtg acgtttgta ggcatcttg gctgagcat tgtgttgag atttgtggc gtgcgtaat tgcacctgt caggcgttt tatctatcg actgagttc atgtgggaa gaagagtcc tggacgatt gcaaccgca agacttatt attgtgttc attgtttta ctgcatttt gttgttatt tcgataggt tttgttttt agagattat ttaatttat aaaagaccg ccaatcaaa aacctttac tggatagtc acttgtttt attctagtg gcgcttcag agtatttat acgatttta agtttttac aacattacg aataatacc gataatagt atccagttt caaatacca ctttgggtg ataattttt gccgcaata tctccgttt ttagtgctg attattaac gaagtgatc aaacagcag gaaataatg gtaaataaa cgattcagg aagcgggcg cgactggac tttgggaca aaattggga atgaattct ccattttag
- >TC006494 RA
tcgctcacc ttgaatagt ggccagaac taaacccaa cgtccttga cggcactaa ccaggcacg tgttggact catacaaat tacactttt gtacacttg gattcacgc aactccacc ctcagtgtg attaaatca ccgaatttc ggggataaa aacgcaaaa acaacgcaa catgtgtga taacgcttg acggttaat tgacagttg acagctgtt caaggtttt gtggttata tcaaaaata atgtaacga attcccgaa attcccgtg tgtggtaaa cagttgggc cgtttcgtg ggttctgtg atgacataa tgtgagacg ggatggagt cgggcgacg agtcaggtc tgagcaccc aggaggccc tccagcggc tccacgccg agatcgagg cggttctgc agcagctga agcagtccc aaaaggcca gaaaatccc gctcctgga tcaaagaca cgttccacc actccagcc aagacacca cgatcaatt gggtgtctg cgagtttcc agttgtggg cggtggcca gcatgttcc ttgctttta taattgaac aaaattttg tcttgtgtg ttgagagta tcacacttt tgctttttc tcctaatta acgtgacgt tggaactgt acgataata aattgaggc atgaggagg tgccaaacc gggtgcagg cggttcttg acacgatta agaggatct cggaggggc gctgggaaa aggagaatt accctaact tgtgttcac cgttttcgc cgtgtatta cgctgcagt ggacctaca gggacaaca aactagtca atctaccgt gggccctat tggtcaagg atgacctaa ttgtgataa ggccgggac aagtgagcc ccggctatt gcgaagccg ttgataaaa cctccgaat ttccgctcc tccacgcca aagaagtat acgcaccgt cgctacaaa gtgccaacg agattttca gtgccccaa aaatacgga aaccgctag aaaataaaa aataccgac tgctggaaa ccccatact tgaacaatt tgcggatct acttggaac agtcactag acaggcctt gcaccctcc tcaacaaac agaggcact tagttatga ttaaaataa tcgagcgtt ttgtcctcc ctatttgtt ttattgtca cgttccttg tgaatttgt tccgatttt tgttcccgt tgggcccca gccagccct cggtgatgt tcctaatcg tccccattt ccacaatcc tacctttgc ttccgttag ttttcccca tggcctgga acctgttga attacttcg gtctggcgc gatttcgct gcattttgg acatttcca aacaaattg aagcctaca aagacaacc ccctcgaag aggacaccg agacttcga ccatcgacc accaagagc tgaaatgtc ggcggcagg aactttgga aaaatttcg tttcgattg ttaaaggcc agccagatt tgttggtac attccgcca atattttgc acgtcttgg gctcggtca ctgcacttg ggtgcgtcg ataaaaaag gaatcctct cgtggccaa accccacgg ctgaaaaaa tctttttcc tccacaata acgaaaacg cttcgcctt cctctagtc tgggcaacg tgggggact tggcccctg acggggaca gcgccggtg gcaccccac accactact tcgaaagtc caaacaccg caactgggg aagttctgg acttgacgc acaatcaca gcgaccctt ttaaactcc atttcgacg accaggagt ggcaccaat acttaacct ccctgaagc cgctaggcc ttgctattt tgttaaaca cttgcaacg ttgaaaccc aggagcatt atgcggcat tttgtagcc acattacgt gcgaggcca tttataacg aaaatctgg ttccggtga gcaaccgac ggtgtctgt gcgagttgg ccaaagaaa ttggatttg tggaccagg cccagaaaa ttttcgcgc ttgaacaac aactctcca cctttcggc acttgcagc cggaaatgg tccgaagtg ataataagt atgcgaggt cgcttcaac tttcaacaa aactcaaat ttccgttcc cgcacatgt ttgctgtgg tggtcaaag agttgagca gtggctcca tgcagttgt tgtctgaag gaacggctg atataattt tggattcgt gcgtggatt attgggacg ggcatgatc tgtgccctt tgactcctt ccgaccgga aaaaaatcc aggattttt accagagga gcagcctca cggcgtatt gtacggcgt tcgcttaca ggcccttaa ataaatgtg ttagtagta aattagggg gagtttatt tggaattgc cgagtgata gtagacact tgtatatga attaccgga gtccgacgc cagtccatt gggactgca agagcgtcc ttgagccga aaattaaga ctcctttgg ggcaatttt ttagcactg actcgcttt tgtacaatg attgtccgt cggttgatg tttccgatg ctgagagtt gcttccaaa tgcagtgta atcaggtct ttatcggaa tggtgacta tgcagtacc aggcattga ccgatatgg tgcagctaa tcgaacagc tggaacgtg cctgcatcc gattcgtcc atttcagca aggaaaacg agctgcgtt cgcgcgttt tcagcgaga aaatgggcc tggaaagcg gctggaatt gccacattt cccttttga gcgaacgca acagcaatt tggagtcgt cccgaacga tgagcttct ccgccccca gtgccataa acatggagc attcggttg tgaagtttg acacggaac aggagcgcc tcaacaaaa attcaagcg atcaaagcg gcgtttact ccacgaaag tgagccacg acagcgtcc agatccaca acgagatca acacgtcgc acgactggc agtctctga gttgcctca cggacagta ccgagcaaa gcgccccaa ttaacttcg atatgagca atcgggcga aactacccc gtggtattg acaaaatcc gcccccata tcgagctaa tcgacaacg tccccttgc tagtgtccc tctttaccg actgcacgc ccagcgcga cccgcgaaa tgctcaaca tcatgcaag actacggcg aaatcgtgt gcataatgg gctcctcgg ccaactgcg agaacgcgg ggattttca tgcaggccg atgcgagca tcgcagtgg accccctgt tcccccaaa tgtgccaga aagtcccag tttacaaga agccaaaaa tgggggttt caccgctcg atctgtcgc gcgacttga actcgatcg cttgctccc tgtgcctca aacgcgagg acccgatct cgttctatc acctagtca tggaggcgc ggcatttcg tcacaatcc tctggagtt cggtccagt tttgggtgt cttgttgcg ttatcttga gtgttctgc agctggtct cggtgctgg tgatgttgc cccccgttt tgacaacgg ggcaagtct tgtggttga cgtgttttg ttgttcctc tgttgagtg tgtcgctga tggggcttc cggtggatc cggaggtca tgaagaagc cgcaaggga agaaccaag tcggggtca atctctata tttcgatgt ttattctgt ggtgttacg gattgaagt tttttccga ctgttgttg ttgttattg tgacgtttg taggcatct tggctgagc attgtgttg agatttgtg gcgtgcgta attgcacct gtcaggcgt tttatctat cgactgagt tcatgtggg aagaagagt cctggacga ttgcaaccg caagactta ttattgtgt tcattgttt tactgcatt ttgttgtta tttcgatag gttttgttt ttagagatt atttaattt ataaaagac cgccaatca aaaaccttt actggatag tcacttgtt ttattctag tggcgcttc agagtattt atacgattt taagttttt acaacatta cgaataata ccgataata gtatccagt ttcaaatac cactttggg tgataattt ttgccgcaa tatctccgt ttttagtgc tgattatta acgaagtga tcaaacagc aggaaataa tggtaaata aacgattca ggaagcggg cgcgactgg actttggga caaaattgg gaatgaatt ctccatttt agcgatacg aagtgaaaa ctatatttt tatatatcg gtgtaacgg aaccagttg actgatttc atttgataa gactggaat gtttgtaaa aaataaagt gttgatttt aag
