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Gene information
General information
TC006481 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10396130..10407244
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006481
    iBeetle screen iB_04153

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen musculature lateral pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: leg musculature seems to be normal
    abdomen musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Adult morphology
    motility decreased - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006481 RA
      MTQSHYSLR WNNHQTHIL AAFDALLQA ETLVDVTLV 
      CAETSVRAH KVVLSACSP FFQRIFSEN PCKHPVIVL 
      KDFSGWEVQ AIVDFMYKG EISVIQEQL QSLIKAAES 
      LQVRGLANQ DPFGVDKES TSIINQTPT PSTSPNDFD 
      RNYFGRFPT PGVTVRTPG ERSSPFSPL SCPSNYEPS 
      VKLPHMSRL SFPESLLPR PECQSPIPR RKQARPRRR 
      SGELCGPQD LTKPSPPPS EDAIPENLS VKKPKEDKA 
      KDEDKIRTP DPSGSPEMD LSMSTGGSK EFRASPPVS 
      LPPTINMKQ EIDSHSPLP FPPMPSVSA LAMTPPHSK 
      FFGLDSPLG LFPPGLDGC RNPLFDMTD PRTTPDSQL 
      FVKKKMGRP KGQHSAPRG GPPRSWTNA ELTEALQHV 
      WNKKMTTSQ ASRIFGIPY NSLLMYVRG KYGKSLKLE 
      QLRKDCIGG PTPSMDILS MGNNNNNSC KSDRDNEAI 
      QPNTRPSST EEPHPQPPV FNPFAHNFY PDFGTGFPI 
      PVSMIHLLP QSEKSRDHY GAQPQGEDD CKSERVKKE 
      MEEEEMFRP PALSVDDRR ELVQQNGQD
    CDS sequence
    • >TC006481 RA
      atgacccag tcccactac tctctgcga tggaacaac 
      catcagacg cacatcctg gcggccttc gacgccctc 
      ctccaggct gagaccctc gtggacgtc accctcgtc 
      tgcgccgag acgtccgtg cgcgcccac aaagtcgtc 
      ctcagcgcg tgctcgccc ttcttccag cggatattc 
      tcggagaat ccctgcaag caccccgtc atcgtcctc 
      aaggacttc agcggctgg gaggtccaa gccatcgtc 
      gattttatg tacaaaggc gaaatcagc gtgatccaa 
      gagcaactc caaagtctg atcaaggcc gccgagagc 
      ctccaagtg cgggggctg gccaaccag gaccccttc 
      ggggtcgac aaagagtcc acgtcgatc attaaccag 
      acgccgacg ccctcgacg tcacccaac gacttcgac 
      aggaattac ttcggaagg tttccgact cctggagtg 
      acggttcgc accccgggg gaacgttcg tcccccttt 
      tcgcccctg tcgtgtccg tcgaactac gagccgtcg 
      gtgaagctc ccccacatg tcccgcctg tccttcccc 
      gagtccctg ctcccccgg cccgaatgc cagtcgccc 
      atcccccgg aggaagcag gcgcggccg cggcggagg 
      tccggcgag ctgtgcggc ccccaggac ctcaccaag 
      ccgtccccg ccgccctcc gaagacgcc atccccgag 
      aatctcagt gttaaaaag ccgaaggaa gacaaggcg 
      aaagacgag gacaaaatt cggaccccg gacccgtcc 
      gggagcccc gaaatggac ctcagcatg tccaccgga 
      ggctccaag gagttccgg gcttcgcca cctgttagt 
      ctgccgccc accatcaac atgaagcaa gaaattgat 
      agccactca cctctaccg ttcccaccc atgccgtcg 
      gtttccgca cttgcgatg actcctccg cacagtaaa 
      ttttttgga ctggactcg cccttgggg ctcttcccc 
      ccggggctg gacggctgc cgcaacccc ctcttcgac 
      atgaccgac ccccggacg acgcccgac tcccagctc 
      ttcgtcaag aaaaaaatg ggccggccc aagggccag 
      cactcggcg ccccgcggg ggcccgccg cgctcgtgg 
      acgaacgcc gagctgacc gaagccctc cagcacgtg 
      tggaacaag aagatgacg acgagtcag gcgtcccgc 
      atcttcggc atcccgtac aactcgctg ctgatgtac 
      gtgcgcggc aagtacggc aagagcctg aagctggag 
      cagctgcgc aaagactgc atcgggggg cccacgccc 
      tcgatggac atcctcagc atggggaac aacaacaac 
      aactcgtgc aagagcgac cgggacaac gaagcgatc 
      cagcccaac accagacca tccagcacg gaggagccg 
      cacccccag ccgcccgtc ttcaacccc ttcgcgcat 
      aacttctac cccgacttc ggcacgggc ttccccatc 
      ccggtcagc atgatccat ctgttgccg cagagcgaa 
      aagagcagg gaccactac ggggcccaa ccgcagggc 
      gaagacgac tgcaaaagc gagcgggtg aagaaggag 
      atggaggag gaggagatg ttcaggcct ccggcgctc 
      tctgtcgac gacaggagg gagctggtc cagcagaac 
      gggcaggac taa
    mRNA sequence
    • >TC006481 RA
      aacgtgaag acaccaaca ggttaatag tgccgtttc 
      ggaggggag ctgcatttt aatgggaaa tgtggcctg 
      ctacagtgt agtggcggc tcgtgacga aatttatac 
      gccgagggc gcagcgaaa taagaaatt caaaattcc 
      ggaggagac gaaagagaa tgacggcgg tttgacgtt 
      agcttcaaa cgagaactt tcggacctg accacgagc 
      tcgcccttt ccacgatga cccagtccc actactctc 
      tgcgatgga acaaccatc agacgcaca tcctggcgg 
      ccttcgacg ccctcctcc aggctgaga ccctcgtgg 
      acgtcaccc tcgtctgcg ccgagacgt ccgtgcgcg 
      cccacaaag tcgtcctca gcgcgtgct cgcccttct 
      tccagcgga tattctcgg agaatccct gcaagcacc 
      ccgtcatcg tcctcaagg acttcagcg gctgggagg 
      tccaagcca tcgtcgatt ttatgtaca aaggcgaaa 
      tcagcgtga tccaagagc aactccaaa gtctgatca 
      aggccgccg agagcctcc aagtgcggg ggctggcca 
      accaggacc ccttcgggg tcgacaaag agtccacgt 
      cgatcatta accagacgc cgacgccct cgacgtcac 
      ccaacgact tcgacagga attacttcg gaaggtttc 
      cgactcctg gagtgacgg ttcgcaccc cgggggaac 
      gttcgtccc ccttttcgc ccctgtcgt gtccgtcga 
      actacgagc cgtcggtga agctccccc acatgtccc 
      gcctgtcct tccccgagt ccctgctcc cccggcccg 
      aatgccagt cgcccatcc cccggagga agcaggcgc 
      ggccgcggc ggaggtccg gcgagctgt gcggccccc 
      aggacctca ccaagccgt ccccgccgc cctccgaag 
      acgccatcc ccgagaatc tcagtgtta aaaagccga 
      aggaagaca aggcgaaag acgaggaca aaattcgga 
      ccccggacc cgtccggga gccccgaaa tggacctca 
      gcatgtcca ccggaggct ccaaggagt tccgggctt 
      cgccacctg ttagtctgc cgcccacca tcaacatga 
      agcaagaaa ttgatagcc actcacctc taccgttcc 
      cacccatgc cgtcggttt ccgcacttg cgatgactc 
      ctccgcaca gtaaatttt ttggactgg actcgccct 
      tggggctct tccccccgg ggctggacg gctgccgca 
      accccctct tcgacatga ccgaccccc ggacgacgc 
      ccgactccc agctcttcg tcaagaaaa aaatgggcc 
      ggcccaagg gccagcact cggcgcccc gcgggggcc 
      cgccgcgct cgtggacga acgccgagc tgaccgaag 
      ccctccagc acgtgtgga acaagaaga tgacgacga 
      gtcaggcgt cccgcatct tcggcatcc cgtacaact 
      cgctgctga tgtacgtgc gcggcaagt acggcaaga 
      gcctgaagc tggagcagc tgcgcaaag actgcatcg 
      gggggccca cgccctcga tggacatcc tcagcatgg 
      ggaacaaca acaacaact cgtgcaaga gcgaccggg 
      acaacgaag cgatccagc ccaacacca gaccatcca 
      gcacggagg agccgcacc cccagccgc ccgtcttca 
      accccttcg cgcataact tctaccccg acttcggca 
      cgggcttcc ccatcccgg tcagcatga tccatctgt 
      tgccgcaga gcgaaaaga gcagggacc actacgggg 
      cccaaccgc agggcgaag acgactgca aaagcgagc 
      gggtgaaga aggagatgg aggaggagg agatgttca 
      ggcctccgg cgctctctg tcgacgaca ggagggagc 
      tggtccagc agaacgggc aggactaaa gagggcgcg 
      cgtgtcgag gcttaaggt tctttttgt ttcggttgg 
      ttgttgttt aaatgttta ttcttagag taataagct 
      gtgcttcct catttttct cattcgttt ccggtatta 
      gaaagaatg ctcgccggg tcgtggccc cgcgacccg 
      gggttttta taagactga tatatcata ttgccaacg 
      ttccaacag tttaacagt ttattttac agactacca 
      ctttacagt tcctcgcat attttaata tacagattt 
      tattatacc agatttcta taatataca aacggtttt 
      ctgtttcga cattcctct acatgcaac aatttttca 
      gctgcgata ttagtaaca gtaattttt tacacgcgt 
      ttgtacagt aaacagatt ataaataat attaaacaa 
      gaatatttt gttctacag aactctact ttcagtatt 
      tttgtaacc tgacccatg aacagacta ttatacaga 
      tttttatac atttacagc agttaatcg ttgatacta 
      acgttggaa ccgactgat gaaattctt tgttgacgc 
      aacgttgtg attgttacc taatttccc acctgattt 
      tttggcaag atgattgaa aaagcggtc tctcattca 
      accggacaa gcctttaga ctaacatga aacccaaaa 
      aacgtttgt acaaaaatt atgtgttga cttagtgta 
      ttcaactcg tagagcaaa ttcggtgtg ttaataaac 
      aaattgtga aaaattgta taaatttag gtcattata 
      ggtatacgg tcaacaaac aatgtacaa atcttctct 
      ctttggctg caaggaaat tcaaccgct tttcatctc 
      gatcgagtc acgctaacg tgacaccag caatttttg 
      tgataacta ttttcttat tgagtcggt ttacagcac 
      tatcaccgc ttggtgcac tcaaatcac tcggatttt 
      gttccgtta cgtccattg cagggtact agtgagttc 
      aaaaacttg cagtttcaa acaaattcg atgacacat 
      cactaggat aataatttc aatagaatc tctgtgtgt 
      gatttcgtt tcttcgatc cctcattgt atgatatta 
      aaaatggaa aataatgta aattaggat tcttgataa 
      aatgtgata acaagtcgc gtctgcggc tcgacattt 
      gttagatat tatatttag tagtagtta ggacgggat 
      tgacaaacg tctcttttc atgtttttg ggcgacttt 
      tgacagtct tgtccgcaa gtgagatat atgttacca 
      ctattcttc tgtttattt attcgattc tccctctct 
      tacgacaaa ggttgtgca cttatagta ttatttttc 
      gcttttatt tataatttt gcgcttcag agttccttg 
      ttttatcaa taaagactc catggaaga acaactcag 
      ctaccaccg acttgctct tccatgccc tacacgaaa 
      taatactac agtaaaata aagaaatgg tgctggtaa 
      ttttaattg ttaagttgt acatataac aaacagaca 
      ttcctatca aagtattat tacttaatt attttgcaa 
      acaagcaat tttattacg agttccttt taattagtt 
      gttttattt tgtatagtc gcacgtgtt agtcgtagg 
      ttttgtttt agtcgcacg gatcttatt tatttattg 
      ttttagcac gagcacaaa atagacaat atcaagtga 
      aaaattaga aaatccgcg aaatgcagg gtatttgat 
      ttttcccct cccccagac agtaccgat aaatactcg 
      agtgcaata ttttcgaaa aattcgttc gtttttaaa 
      tgtataaaa gaattttcg aaattctca aaaaggaac 
      gtatccctg gcataattg tgtataaaa attaatgtt 
      gcaatctgt aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY