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Gene information
General information
TC006471 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10179931..10191417
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006471
    iBeetle screen iB_04151

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006471 RA
      MIGLGYLVN ATKMSMQLV QKVDVAKIM DDMEQYLHY 
      IGGGAGALA LTGVAAASA FYLASRPSS QKPLFPLDE 
      QCLLEAGPE LIRVSRFYK EAKEGKFVS YMFPDAKTL 
      YETFRRGAK ESNNGPCLG WRESYVKPY QWLNYNEAL 
      LRAKNFGAG MVAQGLTPG TNTIIGIYS QNCPEWIIT 
      EQAAYCYSM VLVPLYDTL GPDACAFII KQAEITVVV 
      CEDDQKCNL LLDRSPRCL RKLIVYKDV RPATKQRAK 
      NRGVEIVKF HDVEVLGSK SNHPEVPPK PSDLCTICY 
      TSGTTGNPK GVMLTHENV VASMSAVLM QMGDHKVKS 
      SDVLISFLP LAHMLERCC ENAMYMMGA SVGFFLGDI 
      RRLSDDMKA LKPTVSPAV PRLLNRIYD KVQAEINGS 
      CIKKMLFNM GMSAKESEI KRGILRNNS FWDMLVFRK 
      VQEGMGGRL RLMLVGSAP LAENVLTFM RCALGCLVI 
      EGYGQTECT APITLTIQG DHVPGHVGP PVACCCVKL 
      IDVPEMEYW AKNNQGEVC VKGTNVFQG YYKDPEKTE 
      ETIDEMGWH HTGDVGMWL PNGTLKIID RRKHIFKLS 
      QGEYIVPEK IENIYIRSQ YVSQVYVHG ESLKSCIIA 
      IVVPDVDVI KCWATENGI PGTLSVLCN NPDVKQLIM 
      DDMTAWGKE AGLKSFEQV KDIYLHPDP FSVQNGLLT 
      PTLKSKRPQ LKEYFKPQI EDMYSKLV
    • >TC006471 RB
      MDAIILKTK NFIKQHQKV KRRKRDDDD RRDKHRRWR 
      NMDDMEQYL HYIGGGAGA LALTGVAAA SAFYLASRP 
      SSQKPLFPL DEQCLLEAG PELIRVSRF YKEAKEGKF 
      VSYMFPDAK TLYETFRRG AKESNNGPC LGWRESYVK 
      PYQWLNYNE ALLRAKNFG AGMVAQGLT PGTNTIIGI 
      YSQNCPEWI ITEQAAYCY SMVLVPLYD TLGPDACAF 
      IIKQAEITV VVCEDDQKC NLLLDRSPR CLRKLIVYK 
      DVRPATKQR AKNRGVEIV KFHDVEVLG SKSNHPEVP 
      PKPSDLCTI CYTSGTTGN PKGVMLTHE NVVASMSAV 
      LMQMGDHKV KSSDVLISF LPLAHMLER CCENAMYMM 
      GASVGFFLG DIRRLSDDM KALKPTVSP AVPRLLNRI 
      YDKVQAEIN GSCIKKMLF NMGMSAKES EIKRGILRN 
      NSFWDMLVF RKVQEGMGG RLRLMLVGS APLAENVLT 
      FMRCALGCL VIEGYGQTE CTAPITLTI QGDHVPGHV 
      GPPVACCCV KLIDVPEME YWAKNNQGE VCVKGTNVF 
      QGYYKDPEK TEETIDEMG WHHTGDVGM WLPNGTLKI 
      IDRRKHIFK LSQGEYIVP EKIENIYIR SQYVSQVYV 
      HGESLKSCI IAIVVPDVD VIKCWATEN GIPGTLSVL 
      CNNPDVKQL IMDDMTAWG KEAGLKSFE QVKDIYLHP 
      DPFSVQNGL LTPTLKSKR PQLKEYFKP QIEDMYSKL 
      V
    CDS sequence
    • >TC006471 RA
      atgatcggc ttgggctat ttggtcaat gcgacgaag 
      atgtccatg cagttggtg caaaaggtg gacgtggcc 
      aaaatcatg gacgacatg gagcagtac ctccactac 
      atcggtggc ggcgcgggg gcattggcc ctcaccggc 
      gtggccgca gcttcggcc ttctaccta gccagcaga 
      ccgtcctcc caaaaacca ttgtttcca ctagacgaa 
      caatgcctc ctcgaagcg ggcccggag ctgatccgg 
      gtgtcgcgc ttctacaag gaagccaag gagggcaag 
      ttcgtcagt tatatgttt ccggacgct aagactctc 
      tacgagact ttccggaga ggagcgaaa gaatcaaat 
      aatggtcct tgtttggga tggcgggag tcgtacgtg 
      aaaccgtac cagtggctg aactacaat gaagctctt 
      ctcagggct aaaaacttc ggagcaggg atggtggcc 
      caagggctg actccgggc actaacacc atcatcgga 
      atttactcg cagaattgt cccgaatgg atcattacg 
      gaacaagct gcctattgt tacagcatg gtgttggtg 
      cccctctac gacactctc ggtcctgat gcatgtgcc 
      tttattatt aaacaagct gaaataacc gtggttgtt 
      tgcgaagac gaccaaaaa tgcaacctc ctcctcgac 
      cgctctccg cgttgcctc cgcaagctc atcgtctac 
      aaagacgtg agacctgcg acgaagcaa agggcaaag 
      aatcgagga gtggaaatc gtcaaattc cacgacgtt 
      gaagtttta ggttcgaag tcgaaccat ccggaagtt 
      cctcctaaa ccctcagac ctctgcact atttgttac 
      acgagtggc accaccggc aatcctaaa ggagttatg 
      ctaactcat gaaaatgtt gtggcctct atgagtgct 
      gttttgatg caaatgggc gatcacaag gtgaagagt 
      agcgacgtt cttatctcg ttcctgccg ctagcgcat 
      atgctggag cgttgctgc gagaacgct atgtatatg 
      atgggtgcc tcagtcggt ttctttctc ggggacata 
      aggcgatta tcggacgat atgaaagcc ctgaagcct 
      actgtctcc ccggcagtt cctcgactc cttaacagg 
      atttatgat aaagtccaa gccgagatc aacggcagt 
      tgtattaaa aaaatgtta ttcaatatg gggatgagt 
      gccaaagag agcgaaatc aaacgaggc atcctcagg 
      aataacagt ttttgggac atgttggtg ttcaggaaa 
      gtccaagag gggatgggc ggtcggctt agactaatg 
      ctagtcggg tctgcgccc cttgccgaa aacgtcctc 
      accttcatg cgatgtgct ttaggttgc ttggtgatt 
      gaaggttac ggccaaact gagtgtacc gcacctatc 
      actttgacc atccaaggc gatcacgtg ccgggtcat 
      gttggcccc cctgttgct tgttgctgc gttaaatta 
      atcgatgtg ccggaaatg gaatactgg gcgaagaac 
      aatcaaggg gaggtctgt gttaaaggc accaatgtt 
      tttcaaggt tactataaa gatcctgaa aagactgaa 
      gagacaata gatgagatg ggctggcac catactgga 
      gatgttggg atgtggcta ccaaatggt acccttaaa 
      atcatcgac cggcgaaaa cacatcttt aaactatct 
      caaggcgag tatattgtt cctgaaaaa atcgaaaat 
      atttatatt cggtcacaa tacgtaagc caagtctat 
      gtccacggc gagtctttg aagtcttgc attatcgca 
      attgttgtt cctgacgtc gatgtgatc aaatgttgg 
      gcgacggaa aacggtatt cctggtacc cttagtgtg 
      ctttgcaac aaccctgat gtcaaacaa ctaatcatg 
      gatgacatg accgcttgg ggtaaagaa gcgggtctt 
      aaatcattc gaacaagtc aaagacata tatttacat 
      cccgatccg tttagtgtc caaaacggt ttattgaca 
      ccaacgctg aaatcgaaa cgtccgcaa ctgaaagaa 
      tatttcaaa ccgcaaatt gaagatatg tacagtaaa 
      ttggtctaa
    • >TC006471 RB
      atggacgcg atcatcctc aaaaccaag aacttcatc 
      aaacagcac cagaaggtg aagaggagg aagagggat 
      gacgacgac agaagggac aagcacagg cggtggagg 
      aacatggac gacatggag cagtacctc cactacatc 
      ggtggcggc gcgggggca ttggccctc accggcgtg 
      gccgcagct tcggccttc tacctagcc agcagaccg 
      tcctcccaa aaaccattg tttccacta gacgaacaa 
      tgcctcctc gaagcgggc ccggagctg atccgggtg 
      tcgcgcttc tacaaggaa gccaaggag ggcaagttc 
      gtcagttat atgtttccg gacgctaag actctctac 
      gagactttc cggagagga gcgaaagaa tcaaataat 
      ggtccttgt ttgggatgg cgggagtcg tacgtgaaa 
      ccgtaccag tggctgaac tacaatgaa gctcttctc 
      agggctaaa aacttcgga gcagggatg gtggcccaa 
      gggctgact ccgggcact aacaccatc atcggaatt 
      tactcgcag aattgtccc gaatggatc attacggaa 
      caagctgcc tattgttac agcatggtg ttggtgccc 
      ctctacgac actctcggt cctgatgca tgtgccttt 
      attattaaa caagctgaa ataaccgtg gttgtttgc 
      gaagacgac caaaaatgc aacctcctc ctcgaccgc 
      tctccgcgt tgcctccgc aagctcatc gtctacaaa 
      gacgtgaga cctgcgacg aagcaaagg gcaaagaat 
      cgaggagtg gaaatcgtc aaattccac gacgttgaa 
      gttttaggt tcgaagtcg aaccatccg gaagttcct 
      cctaaaccc tcagacctc tgcactatt tgttacacg 
      agtggcacc accggcaat cctaaagga gttatgcta 
      actcatgaa aatgttgtg gcctctatg agtgctgtt 
      ttgatgcaa atgggcgat cacaaggtg aagagtagc 
      gacgttctt atctcgttc ctgccgcta gcgcatatg 
      ctggagcgt tgctgcgag aacgctatg tatatgatg 
      ggtgcctca gtcggtttc tttctcggg gacataagg 
      cgattatcg gacgatatg aaagccctg aagcctact 
      gtctccccg gcagttcct cgactcctt aacaggatt 
      tatgataaa gtccaagcc gagatcaac ggcagttgt 
      attaaaaaa atgttattc aatatgggg atgagtgcc 
      aaagagagc gaaatcaaa cgaggcatc ctcaggaat 
      aacagtttt tgggacatg ttggtgttc aggaaagtc 
      caagagggg atgggcggt cggcttaga ctaatgcta 
      gtcgggtct gcgcccctt gccgaaaac gtcctcacc 
      ttcatgcga tgtgcttta ggttgcttg gtgattgaa 
      ggttacggc caaactgag tgtaccgca cctatcact 
      ttgaccatc caaggcgat cacgtgccg ggtcatgtt 
      ggcccccct gttgcttgt tgctgcgtt aaattaatc 
      gatgtgccg gaaatggaa tactgggcg aagaacaat 
      caaggggag gtctgtgtt aaaggcacc aatgttttt 
      caaggttac tataaagat cctgaaaag actgaagag 
      acaatagat gagatgggc tggcaccat actggagat 
      gttgggatg tggctacca aatggtacc cttaaaatc 
      atcgaccgg cgaaaacac atctttaaa ctatctcaa 
      ggcgagtat attgttcct gaaaaaatc gaaaatatt 
      tatattcgg tcacaatac gtaagccaa gtctatgtc 
      cacggcgag tctttgaag tcttgcatt atcgcaatt 
      gttgttcct gacgtcgat gtgatcaaa tgttgggcg 
      acggaaaac ggtattcct ggtaccctt agtgtgctt 
      tgcaacaac cctgatgtc aaacaacta atcatggat 
      gacatgacc gcttggggt aaagaagcg ggtcttaaa 
      tcattcgaa caagtcaaa gacatatat ttacatccc 
      gatccgttt agtgtccaa aacggttta ttgacacca 
      acgctgaaa tcgaaacgt ccgcaactg aaagaatat 
      ttcaaaccg caaattgaa gatatgtac agtaaattg 
      gtctaa
    mRNA sequence
    • >TC006471 RA
      gggcacaaa tagccggcg taattccga ctctggggc 
      tattttccc ctcaataaa accacaatt agccacgtc 
      agagcagaa ttcctattg aaattttaa tacaatatt 
      gattaataa gtctcgcca ccacctacg tttccatcc 
      cttgacgtt ccggagggg tgcttccag tttgaccgt 
      gaacactgc aaagcgtgg tcgaattcc ggggctgtg 
      atttcgatg attggtgtt aattgagac gatttcggc 
      atgaaatcg ctcaacgag tgctttatt gcgagcggc 
      tagttgtta gtgtttact aaagtgata tgttcgggt 
      gataatcgg atgccaatt acttatgat cggcttggg 
      ctatttggt caatgcgac gaagatgtc catgcagtt 
      ggtgcaaaa ggtggacgt ggccaaaat catggacga 
      catggagca gtacctcca ctacatcgg tggcggcgc 
      gggggcatt ggccctcac cggcgtggc cgcagcttc 
      ggccttcta cctagccag cagaccgtc ctcccaaaa 
      accattgtt tccactaga cgaacaatg cctcctcga 
      agcgggccc ggagctgat ccgggtgtc gcgcttcta 
      caaggaagc caaggaggg caagttcgt cagttatat 
      gtttccgga cgctaagac tctctacga gactttccg 
      gagaggagc gaaagaatc aaataatgg tccttgttt 
      gggatggcg ggagtcgta cgtgaaacc gtaccagtg 
      gctgaacta caatgaagc tcttctcag ggctaaaaa 
      cttcggagc agggatggt ggcccaagg gctgactcc 
      gggcactaa caccatcat cggaattta ctcgcagaa 
      ttgtcccga atggatcat tacggaaca agctgccta 
      ttgttacag catggtgtt ggtgcccct ctacgacac 
      tctcggtcc tgatgcatg tgcctttat tattaaaca 
      agctgaaat aaccgtggt tgtttgcga agacgacca 
      aaaatgcaa cctcctcct cgaccgctc tccgcgttg 
      cctccgcaa gctcatcgt ctacaaaga cgtgagacc 
      tgcgacgaa gcaaagggc aaagaatcg aggagtgga 
      aatcgtcaa attccacga cgttgaagt tttaggttc 
      gaagtcgaa ccatccgga agttcctcc taaaccctc 
      agacctctg cactatttg ttacacgag tggcaccac 
      cggcaatcc taaaggagt tatgctaac tcatgaaaa 
      tgttgtggc ctctatgag tgctgtttt gatgcaaat 
      gggcgatca caaggtgaa gagtagcga cgttcttat 
      ctcgttcct gccgctagc gcatatgct ggagcgttg 
      ctgcgagaa cgctatgta tatgatggg tgcctcagt 
      cggtttctt tctcgggga cataaggcg attatcgga 
      cgatatgaa agccctgaa gcctactgt ctccccggc 
      agttcctcg actccttaa caggattta tgataaagt 
      ccaagccga gatcaacgg cagttgtat taaaaaaat 
      gttattcaa tatggggat gagtgccaa agagagcga 
      aatcaaacg aggcatcct caggaataa cagtttttg 
      ggacatgtt ggtgttcag gaaagtcca agaggggat 
      gggcggtcg gcttagact aatgctagt cgggtctgc 
      gccccttgc cgaaaacgt cctcacctt catgcgatg 
      tgctttagg ttgcttggt gattgaagg ttacggcca 
      aactgagtg taccgcacc tatcacttt gaccatcca 
      aggcgatca cgtgccggg tcatgttgg cccccctgt 
      tgcttgttg ctgcgttaa attaatcga tgtgccgga 
      aatggaata ctgggcgaa gaacaatca aggggaggt 
      ctgtgttaa aggcaccaa tgtttttca aggttacta 
      taaagatcc tgaaaagac tgaagagac aatagatga 
      gatgggctg gcaccatac tggagatgt tgggatgtg 
      gctaccaaa tggtaccct taaaatcat cgaccggcg 
      aaaacacat ctttaaact atctcaagg cgagtatat 
      tgttcctga aaaaatcga aaatattta tattcggtc 
      acaatacgt aagccaagt ctatgtcca cggcgagtc 
      tttgaagtc ttgcattat cgcaattgt tgttcctga 
      cgtcgatgt gatcaaatg ttgggcgac ggaaaacgg 
      tattcctgg tacccttag tgtgctttg caacaaccc 
      tgatgtcaa acaactaat catggatga catgaccgc 
      ttggggtaa agaagcggg tcttaaatc attcgaaca 
      agtcaaaga catatattt acatcccga tccgtttag 
      tgtccaaaa cggtttatt gacaccaac gctgaaatc 
      gaaacgtcc gcaactgaa agaatattt caaaccgca 
      aattgaaga tatgtacag taaattggt ctaaaacga 
      acaagtatt aggaatttt cttagatct aggatgcac 
      caaagcaaa tttctcatt tgaagactt gtttatagc 
      aatatctat cgagtcaat cgagaacgc aataaatac 
      cttgggagg ttgccaaaa caattattt tgatatttc 
      gttcggttt tatcaacaa atatgcatt atcttgcct 
      tttagatat aggtttaac accgtcaat ctgactctc 
      ttaggttag aacattgtt tatctgaca acaatttta 
      tttattgtt cgttgttgt attctaaac aacccttta 
      ataaagtta tttgaaaaa a
    • >TC006471 RB
      gggcacaaa tagccggcg taattccga ctctggggc 
      tattttccc ctcaataaa accacaatt agccacgtc 
      agagcagaa ttcctattg aaattttaa tacaatatt 
      gattaataa gtctcgcca ccacctacg tttccatcc 
      cttgacgtt ccggagggg tgcttccag tttgaccgt 
      gaacactgc aaagcgtgg tcgaattcc ggggctgtg 
      atttcgatg attggtgtt aattgagac gatttcggc 
      atgaaatcg ctcaacgag tgctttatt gcgagcggc 
      tagttgtta gtgtttact aaagtgata tgttcgggt 
      gataatcgg atgccaatt acttatgat cggcttggg 
      ctatttggt caatgcgac gaagatgtc catgcagtt 
      ggtgcaaaa ggtggacgt ggccaaaat cattgtcgg 
      tacctcgac ggctccgac ggttctgcg agtaccgta 
      tttcggtgt ttacgccaa acaaagatc cgcggttat 
      tggccttgt ccgaattat tcactcgca gataaattt 
      tatcgcgga tttaattaa tcatcacct gtggcccca 
      ctaggcgca cttcgctag ttcttcatc cagaatcaa 
      aaagtcccc tctctcgtg atctcgttc accgttacc 
      atattcgac ttctccaga tcgaactat gaggtgttg 
      tagtgcgtg agggatgga cgcgatcat cctcaaaac 
      caagaactt catcaaaca gcaccagaa ggtgaagag 
      gaggaagag ggatgacga cgacagaag ggacaagca 
      caggcggtg gaggaacat ggacgacat ggagcagta 
      cctccacta catcggtgg cggcgcggg ggcattggc 
      cctcaccgg cgtggccgc agcttcggc cttctacct 
      agccagcag accgtcctc ccaaaaacc attgtttcc 
      actagacga acaatgcct cctcgaagc gggcccgga 
      gctgatccg ggtgtcgcg cttctacaa ggaagccaa 
      ggagggcaa gttcgtcag ttatatgtt tccggacgc 
      taagactct ctacgagac tttccggag aggagcgaa 
      agaatcaaa taatggtcc ttgtttggg atggcggga 
      gtcgtacgt gaaaccgta ccagtggct gaactacaa 
      tgaagctct tctcagggc taaaaactt cggagcagg 
      gatggtggc ccaagggct gactccggg cactaacac 
      catcatcgg aatttactc gcagaattg tcccgaatg 
      gatcattac ggaacaagc tgcctattg ttacagcat 
      ggtgttggt gcccctcta cgacactct cggtcctga 
      tgcatgtgc ctttattat taaacaagc tgaaataac 
      cgtggttgt ttgcgaaga cgaccaaaa atgcaacct 
      cctcctcga ccgctctcc gcgttgcct ccgcaagct 
      catcgtcta caaagacgt gagacctgc gacgaagca 
      aagggcaaa gaatcgagg agtggaaat cgtcaaatt 
      ccacgacgt tgaagtttt aggttcgaa gtcgaacca 
      tccggaagt tcctcctaa accctcaga cctctgcac 
      tatttgtta cacgagtgg caccaccgg caatcctaa 
      aggagttat gctaactca tgaaaatgt tgtggcctc 
      tatgagtgc tgttttgat gcaaatggg cgatcacaa 
      ggtgaagag tagcgacgt tcttatctc gttcctgcc 
      gctagcgca tatgctgga gcgttgctg cgagaacgc 
      tatgtatat gatgggtgc ctcagtcgg tttctttct 
      cggggacat aaggcgatt atcggacga tatgaaagc 
      cctgaagcc tactgtctc cccggcagt tcctcgact 
      ccttaacag gatttatga taaagtcca agccgagat 
      caacggcag ttgtattaa aaaaatgtt attcaatat 
      ggggatgag tgccaaaga gagcgaaat caaacgagg 
      catcctcag gaataacag tttttggga catgttggt 
      gttcaggaa agtccaaga ggggatggg cggtcggct 
      tagactaat gctagtcgg gtctgcgcc ccttgccga 
      aaacgtcct caccttcat gcgatgtgc tttaggttg 
      cttggtgat tgaaggtta cggccaaac tgagtgtac 
      cgcacctat cactttgac catccaagg cgatcacgt 
      gccgggtca tgttggccc ccctgttgc ttgttgctg 
      cgttaaatt aatcgatgt gccggaaat ggaatactg 
      ggcgaagaa caatcaagg ggaggtctg tgttaaagg 
      caccaatgt ttttcaagg ttactataa agatcctga 
      aaagactga agagacaat agatgagat gggctggca 
      ccatactgg agatgttgg gatgtggct accaaatgg 
      tacccttaa aatcatcga ccggcgaaa acacatctt 
      taaactatc tcaaggcga gtatattgt tcctgaaaa 
      aatcgaaaa tatttatat tcggtcaca atacgtaag 
      ccaagtcta tgtccacgg cgagtcttt gaagtcttg 
      cattatcgc aattgttgt tcctgacgt cgatgtgat 
      caaatgttg ggcgacgga aaacggtat tcctggtac 
      ccttagtgt gctttgcaa caaccctga tgtcaaaca 
      actaatcat ggatgacat gaccgcttg gggtaaaga 
      agcgggtct taaatcatt cgaacaagt caaagacat 
      atatttaca tcccgatcc gtttagtgt ccaaaacgg 
      tttattgac accaacgct gaaatcgaa acgtccgca 
      actgaaaga atatttcaa accgcaaat tgaagatat 
      gtacagtaa attggtcta aaacgaaca agtattagg 
      aattttctt agatctagg atgcaccaa agcaaattt 
      ctcatttga agacttgtt tatagcaat atctatcga 
      gtcaatcga gaacgcaat aaatacctt gggaggttg 
      ccaaaacaa ttattttga tatttcgtt cggttttat 
      caacaaata tgcattatc ttgcctttt agatatagg 
      tttaacacc gtcaatctg actctctta ggttagaac 
      attgtttat ctgacaaca attttattt attgttcgt 
      tgttgtatt ctaaacaac cctttaata aagttattt 
      gaaaaaa
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