| General information | |||
| TC006471 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 10179931..10191417 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006471 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04151 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC006471 RA
MIGLGYLVN ATKMSMQLV QKVDVAKIM DDMEQYLHY IGGGAGALA LTGVAAASA FYLASRPSS QKPLFPLDE QCLLEAGPE LIRVSRFYK EAKEGKFVS YMFPDAKTL YETFRRGAK ESNNGPCLG WRESYVKPY QWLNYNEAL LRAKNFGAG MVAQGLTPG TNTIIGIYS QNCPEWIIT EQAAYCYSM VLVPLYDTL GPDACAFII KQAEITVVV CEDDQKCNL LLDRSPRCL RKLIVYKDV RPATKQRAK NRGVEIVKF HDVEVLGSK SNHPEVPPK PSDLCTICY TSGTTGNPK GVMLTHENV VASMSAVLM QMGDHKVKS SDVLISFLP LAHMLERCC ENAMYMMGA SVGFFLGDI RRLSDDMKA LKPTVSPAV PRLLNRIYD KVQAEINGS CIKKMLFNM GMSAKESEI KRGILRNNS FWDMLVFRK VQEGMGGRL RLMLVGSAP LAENVLTFM RCALGCLVI EGYGQTECT APITLTIQG DHVPGHVGP PVACCCVKL IDVPEMEYW AKNNQGEVC VKGTNVFQG YYKDPEKTE ETIDEMGWH HTGDVGMWL PNGTLKIID RRKHIFKLS QGEYIVPEK IENIYIRSQ YVSQVYVHG ESLKSCIIA IVVPDVDVI KCWATENGI PGTLSVLCN NPDVKQLIM DDMTAWGKE AGLKSFEQV KDIYLHPDP FSVQNGLLT PTLKSKRPQ LKEYFKPQI EDMYSKLV
- >TC006471 RB
MDAIILKTK NFIKQHQKV KRRKRDDDD RRDKHRRWR NMDDMEQYL HYIGGGAGA LALTGVAAA SAFYLASRP SSQKPLFPL DEQCLLEAG PELIRVSRF YKEAKEGKF VSYMFPDAK TLYETFRRG AKESNNGPC LGWRESYVK PYQWLNYNE ALLRAKNFG AGMVAQGLT PGTNTIIGI YSQNCPEWI ITEQAAYCY SMVLVPLYD TLGPDACAF IIKQAEITV VVCEDDQKC NLLLDRSPR CLRKLIVYK DVRPATKQR AKNRGVEIV KFHDVEVLG SKSNHPEVP PKPSDLCTI CYTSGTTGN PKGVMLTHE NVVASMSAV LMQMGDHKV KSSDVLISF LPLAHMLER CCENAMYMM GASVGFFLG DIRRLSDDM KALKPTVSP AVPRLLNRI YDKVQAEIN GSCIKKMLF NMGMSAKES EIKRGILRN NSFWDMLVF RKVQEGMGG RLRLMLVGS APLAENVLT FMRCALGCL VIEGYGQTE CTAPITLTI QGDHVPGHV GPPVACCCV KLIDVPEME YWAKNNQGE VCVKGTNVF QGYYKDPEK TEETIDEMG WHHTGDVGM WLPNGTLKI IDRRKHIFK LSQGEYIVP EKIENIYIR SQYVSQVYV HGESLKSCI IAIVVPDVD VIKCWATEN GIPGTLSVL CNNPDVKQL IMDDMTAWG KEAGLKSFE QVKDIYLHP DPFSVQNGL LTPTLKSKR PQLKEYFKP QIEDMYSKL V
- >TC006471 RA
atgatcggc ttgggctat ttggtcaat gcgacgaag atgtccatg cagttggtg caaaaggtg gacgtggcc aaaatcatg gacgacatg gagcagtac ctccactac atcggtggc ggcgcgggg gcattggcc ctcaccggc gtggccgca gcttcggcc ttctaccta gccagcaga ccgtcctcc caaaaacca ttgtttcca ctagacgaa caatgcctc ctcgaagcg ggcccggag ctgatccgg gtgtcgcgc ttctacaag gaagccaag gagggcaag ttcgtcagt tatatgttt ccggacgct aagactctc tacgagact ttccggaga ggagcgaaa gaatcaaat aatggtcct tgtttggga tggcgggag tcgtacgtg aaaccgtac cagtggctg aactacaat gaagctctt ctcagggct aaaaacttc ggagcaggg atggtggcc caagggctg actccgggc actaacacc atcatcgga atttactcg cagaattgt cccgaatgg atcattacg gaacaagct gcctattgt tacagcatg gtgttggtg cccctctac gacactctc ggtcctgat gcatgtgcc tttattatt aaacaagct gaaataacc gtggttgtt tgcgaagac gaccaaaaa tgcaacctc ctcctcgac cgctctccg cgttgcctc cgcaagctc atcgtctac aaagacgtg agacctgcg acgaagcaa agggcaaag aatcgagga gtggaaatc gtcaaattc cacgacgtt gaagtttta ggttcgaag tcgaaccat ccggaagtt cctcctaaa ccctcagac ctctgcact atttgttac acgagtggc accaccggc aatcctaaa ggagttatg ctaactcat gaaaatgtt gtggcctct atgagtgct gttttgatg caaatgggc gatcacaag gtgaagagt agcgacgtt cttatctcg ttcctgccg ctagcgcat atgctggag cgttgctgc gagaacgct atgtatatg atgggtgcc tcagtcggt ttctttctc ggggacata aggcgatta tcggacgat atgaaagcc ctgaagcct actgtctcc ccggcagtt cctcgactc cttaacagg atttatgat aaagtccaa gccgagatc aacggcagt tgtattaaa aaaatgtta ttcaatatg gggatgagt gccaaagag agcgaaatc aaacgaggc atcctcagg aataacagt ttttgggac atgttggtg ttcaggaaa gtccaagag gggatgggc ggtcggctt agactaatg ctagtcggg tctgcgccc cttgccgaa aacgtcctc accttcatg cgatgtgct ttaggttgc ttggtgatt gaaggttac ggccaaact gagtgtacc gcacctatc actttgacc atccaaggc gatcacgtg ccgggtcat gttggcccc cctgttgct tgttgctgc gttaaatta atcgatgtg ccggaaatg gaatactgg gcgaagaac aatcaaggg gaggtctgt gttaaaggc accaatgtt tttcaaggt tactataaa gatcctgaa aagactgaa gagacaata gatgagatg ggctggcac catactgga gatgttggg atgtggcta ccaaatggt acccttaaa atcatcgac cggcgaaaa cacatcttt aaactatct caaggcgag tatattgtt cctgaaaaa atcgaaaat atttatatt cggtcacaa tacgtaagc caagtctat gtccacggc gagtctttg aagtcttgc attatcgca attgttgtt cctgacgtc gatgtgatc aaatgttgg gcgacggaa aacggtatt cctggtacc cttagtgtg ctttgcaac aaccctgat gtcaaacaa ctaatcatg gatgacatg accgcttgg ggtaaagaa gcgggtctt aaatcattc gaacaagtc aaagacata tatttacat cccgatccg tttagtgtc caaaacggt ttattgaca ccaacgctg aaatcgaaa cgtccgcaa ctgaaagaa tatttcaaa ccgcaaatt gaagatatg tacagtaaa ttggtctaa
- >TC006471 RB
atggacgcg atcatcctc aaaaccaag aacttcatc aaacagcac cagaaggtg aagaggagg aagagggat gacgacgac agaagggac aagcacagg cggtggagg aacatggac gacatggag cagtacctc cactacatc ggtggcggc gcgggggca ttggccctc accggcgtg gccgcagct tcggccttc tacctagcc agcagaccg tcctcccaa aaaccattg tttccacta gacgaacaa tgcctcctc gaagcgggc ccggagctg atccgggtg tcgcgcttc tacaaggaa gccaaggag ggcaagttc gtcagttat atgtttccg gacgctaag actctctac gagactttc cggagagga gcgaaagaa tcaaataat ggtccttgt ttgggatgg cgggagtcg tacgtgaaa ccgtaccag tggctgaac tacaatgaa gctcttctc agggctaaa aacttcgga gcagggatg gtggcccaa gggctgact ccgggcact aacaccatc atcggaatt tactcgcag aattgtccc gaatggatc attacggaa caagctgcc tattgttac agcatggtg ttggtgccc ctctacgac actctcggt cctgatgca tgtgccttt attattaaa caagctgaa ataaccgtg gttgtttgc gaagacgac caaaaatgc aacctcctc ctcgaccgc tctccgcgt tgcctccgc aagctcatc gtctacaaa gacgtgaga cctgcgacg aagcaaagg gcaaagaat cgaggagtg gaaatcgtc aaattccac gacgttgaa gttttaggt tcgaagtcg aaccatccg gaagttcct cctaaaccc tcagacctc tgcactatt tgttacacg agtggcacc accggcaat cctaaagga gttatgcta actcatgaa aatgttgtg gcctctatg agtgctgtt ttgatgcaa atgggcgat cacaaggtg aagagtagc gacgttctt atctcgttc ctgccgcta gcgcatatg ctggagcgt tgctgcgag aacgctatg tatatgatg ggtgcctca gtcggtttc tttctcggg gacataagg cgattatcg gacgatatg aaagccctg aagcctact gtctccccg gcagttcct cgactcctt aacaggatt tatgataaa gtccaagcc gagatcaac ggcagttgt attaaaaaa atgttattc aatatgggg atgagtgcc aaagagagc gaaatcaaa cgaggcatc ctcaggaat aacagtttt tgggacatg ttggtgttc aggaaagtc caagagggg atgggcggt cggcttaga ctaatgcta gtcgggtct gcgcccctt gccgaaaac gtcctcacc ttcatgcga tgtgcttta ggttgcttg gtgattgaa ggttacggc caaactgag tgtaccgca cctatcact ttgaccatc caaggcgat cacgtgccg ggtcatgtt ggcccccct gttgcttgt tgctgcgtt aaattaatc gatgtgccg gaaatggaa tactgggcg aagaacaat caaggggag gtctgtgtt aaaggcacc aatgttttt caaggttac tataaagat cctgaaaag actgaagag acaatagat gagatgggc tggcaccat actggagat gttgggatg tggctacca aatggtacc cttaaaatc atcgaccgg cgaaaacac atctttaaa ctatctcaa ggcgagtat attgttcct gaaaaaatc gaaaatatt tatattcgg tcacaatac gtaagccaa gtctatgtc cacggcgag tctttgaag tcttgcatt atcgcaatt gttgttcct gacgtcgat gtgatcaaa tgttgggcg acggaaaac ggtattcct ggtaccctt agtgtgctt tgcaacaac cctgatgtc aaacaacta atcatggat gacatgacc gcttggggt aaagaagcg ggtcttaaa tcattcgaa caagtcaaa gacatatat ttacatccc gatccgttt agtgtccaa aacggttta ttgacacca acgctgaaa tcgaaacgt ccgcaactg aaagaatat ttcaaaccg caaattgaa gatatgtac agtaaattg gtctaa
- >TC006471 RA
gggcacaaa tagccggcg taattccga ctctggggc tattttccc ctcaataaa accacaatt agccacgtc agagcagaa ttcctattg aaattttaa tacaatatt gattaataa gtctcgcca ccacctacg tttccatcc cttgacgtt ccggagggg tgcttccag tttgaccgt gaacactgc aaagcgtgg tcgaattcc ggggctgtg atttcgatg attggtgtt aattgagac gatttcggc atgaaatcg ctcaacgag tgctttatt gcgagcggc tagttgtta gtgtttact aaagtgata tgttcgggt gataatcgg atgccaatt acttatgat cggcttggg ctatttggt caatgcgac gaagatgtc catgcagtt ggtgcaaaa ggtggacgt ggccaaaat catggacga catggagca gtacctcca ctacatcgg tggcggcgc gggggcatt ggccctcac cggcgtggc cgcagcttc ggccttcta cctagccag cagaccgtc ctcccaaaa accattgtt tccactaga cgaacaatg cctcctcga agcgggccc ggagctgat ccgggtgtc gcgcttcta caaggaagc caaggaggg caagttcgt cagttatat gtttccgga cgctaagac tctctacga gactttccg gagaggagc gaaagaatc aaataatgg tccttgttt gggatggcg ggagtcgta cgtgaaacc gtaccagtg gctgaacta caatgaagc tcttctcag ggctaaaaa cttcggagc agggatggt ggcccaagg gctgactcc gggcactaa caccatcat cggaattta ctcgcagaa ttgtcccga atggatcat tacggaaca agctgccta ttgttacag catggtgtt ggtgcccct ctacgacac tctcggtcc tgatgcatg tgcctttat tattaaaca agctgaaat aaccgtggt tgtttgcga agacgacca aaaatgcaa cctcctcct cgaccgctc tccgcgttg cctccgcaa gctcatcgt ctacaaaga cgtgagacc tgcgacgaa gcaaagggc aaagaatcg aggagtgga aatcgtcaa attccacga cgttgaagt tttaggttc gaagtcgaa ccatccgga agttcctcc taaaccctc agacctctg cactatttg ttacacgag tggcaccac cggcaatcc taaaggagt tatgctaac tcatgaaaa tgttgtggc ctctatgag tgctgtttt gatgcaaat gggcgatca caaggtgaa gagtagcga cgttcttat ctcgttcct gccgctagc gcatatgct ggagcgttg ctgcgagaa cgctatgta tatgatggg tgcctcagt cggtttctt tctcgggga cataaggcg attatcgga cgatatgaa agccctgaa gcctactgt ctccccggc agttcctcg actccttaa caggattta tgataaagt ccaagccga gatcaacgg cagttgtat taaaaaaat gttattcaa tatggggat gagtgccaa agagagcga aatcaaacg aggcatcct caggaataa cagtttttg ggacatgtt ggtgttcag gaaagtcca agaggggat gggcggtcg gcttagact aatgctagt cgggtctgc gccccttgc cgaaaacgt cctcacctt catgcgatg tgctttagg ttgcttggt gattgaagg ttacggcca aactgagtg taccgcacc tatcacttt gaccatcca aggcgatca cgtgccggg tcatgttgg cccccctgt tgcttgttg ctgcgttaa attaatcga tgtgccgga aatggaata ctgggcgaa gaacaatca aggggaggt ctgtgttaa aggcaccaa tgtttttca aggttacta taaagatcc tgaaaagac tgaagagac aatagatga gatgggctg gcaccatac tggagatgt tgggatgtg gctaccaaa tggtaccct taaaatcat cgaccggcg aaaacacat ctttaaact atctcaagg cgagtatat tgttcctga aaaaatcga aaatattta tattcggtc acaatacgt aagccaagt ctatgtcca cggcgagtc tttgaagtc ttgcattat cgcaattgt tgttcctga cgtcgatgt gatcaaatg ttgggcgac ggaaaacgg tattcctgg tacccttag tgtgctttg caacaaccc tgatgtcaa acaactaat catggatga catgaccgc ttggggtaa agaagcggg tcttaaatc attcgaaca agtcaaaga catatattt acatcccga tccgtttag tgtccaaaa cggtttatt gacaccaac gctgaaatc gaaacgtcc gcaactgaa agaatattt caaaccgca aattgaaga tatgtacag taaattggt ctaaaacga acaagtatt aggaatttt cttagatct aggatgcac caaagcaaa tttctcatt tgaagactt gtttatagc aatatctat cgagtcaat cgagaacgc aataaatac cttgggagg ttgccaaaa caattattt tgatatttc gttcggttt tatcaacaa atatgcatt atcttgcct tttagatat aggtttaac accgtcaat ctgactctc ttaggttag aacattgtt tatctgaca acaatttta tttattgtt cgttgttgt attctaaac aacccttta ataaagtta tttgaaaaa a
- >TC006471 RB
gggcacaaa tagccggcg taattccga ctctggggc tattttccc ctcaataaa accacaatt agccacgtc agagcagaa ttcctattg aaattttaa tacaatatt gattaataa gtctcgcca ccacctacg tttccatcc cttgacgtt ccggagggg tgcttccag tttgaccgt gaacactgc aaagcgtgg tcgaattcc ggggctgtg atttcgatg attggtgtt aattgagac gatttcggc atgaaatcg ctcaacgag tgctttatt gcgagcggc tagttgtta gtgtttact aaagtgata tgttcgggt gataatcgg atgccaatt acttatgat cggcttggg ctatttggt caatgcgac gaagatgtc catgcagtt ggtgcaaaa ggtggacgt ggccaaaat cattgtcgg tacctcgac ggctccgac ggttctgcg agtaccgta tttcggtgt ttacgccaa acaaagatc cgcggttat tggccttgt ccgaattat tcactcgca gataaattt tatcgcgga tttaattaa tcatcacct gtggcccca ctaggcgca cttcgctag ttcttcatc cagaatcaa aaagtcccc tctctcgtg atctcgttc accgttacc atattcgac ttctccaga tcgaactat gaggtgttg tagtgcgtg agggatgga cgcgatcat cctcaaaac caagaactt catcaaaca gcaccagaa ggtgaagag gaggaagag ggatgacga cgacagaag ggacaagca caggcggtg gaggaacat ggacgacat ggagcagta cctccacta catcggtgg cggcgcggg ggcattggc cctcaccgg cgtggccgc agcttcggc cttctacct agccagcag accgtcctc ccaaaaacc attgtttcc actagacga acaatgcct cctcgaagc gggcccgga gctgatccg ggtgtcgcg cttctacaa ggaagccaa ggagggcaa gttcgtcag ttatatgtt tccggacgc taagactct ctacgagac tttccggag aggagcgaa agaatcaaa taatggtcc ttgtttggg atggcggga gtcgtacgt gaaaccgta ccagtggct gaactacaa tgaagctct tctcagggc taaaaactt cggagcagg gatggtggc ccaagggct gactccggg cactaacac catcatcgg aatttactc gcagaattg tcccgaatg gatcattac ggaacaagc tgcctattg ttacagcat ggtgttggt gcccctcta cgacactct cggtcctga tgcatgtgc ctttattat taaacaagc tgaaataac cgtggttgt ttgcgaaga cgaccaaaa atgcaacct cctcctcga ccgctctcc gcgttgcct ccgcaagct catcgtcta caaagacgt gagacctgc gacgaagca aagggcaaa gaatcgagg agtggaaat cgtcaaatt ccacgacgt tgaagtttt aggttcgaa gtcgaacca tccggaagt tcctcctaa accctcaga cctctgcac tatttgtta cacgagtgg caccaccgg caatcctaa aggagttat gctaactca tgaaaatgt tgtggcctc tatgagtgc tgttttgat gcaaatggg cgatcacaa ggtgaagag tagcgacgt tcttatctc gttcctgcc gctagcgca tatgctgga gcgttgctg cgagaacgc tatgtatat gatgggtgc ctcagtcgg tttctttct cggggacat aaggcgatt atcggacga tatgaaagc cctgaagcc tactgtctc cccggcagt tcctcgact ccttaacag gatttatga taaagtcca agccgagat caacggcag ttgtattaa aaaaatgtt attcaatat ggggatgag tgccaaaga gagcgaaat caaacgagg catcctcag gaataacag tttttggga catgttggt gttcaggaa agtccaaga ggggatggg cggtcggct tagactaat gctagtcgg gtctgcgcc ccttgccga aaacgtcct caccttcat gcgatgtgc tttaggttg cttggtgat tgaaggtta cggccaaac tgagtgtac cgcacctat cactttgac catccaagg cgatcacgt gccgggtca tgttggccc ccctgttgc ttgttgctg cgttaaatt aatcgatgt gccggaaat ggaatactg ggcgaagaa caatcaagg ggaggtctg tgttaaagg caccaatgt ttttcaagg ttactataa agatcctga aaagactga agagacaat agatgagat gggctggca ccatactgg agatgttgg gatgtggct accaaatgg tacccttaa aatcatcga ccggcgaaa acacatctt taaactatc tcaaggcga gtatattgt tcctgaaaa aatcgaaaa tatttatat tcggtcaca atacgtaag ccaagtcta tgtccacgg cgagtcttt gaagtcttg cattatcgc aattgttgt tcctgacgt cgatgtgat caaatgttg ggcgacgga aaacggtat tcctggtac ccttagtgt gctttgcaa caaccctga tgtcaaaca actaatcat ggatgacat gaccgcttg gggtaaaga agcgggtct taaatcatt cgaacaagt caaagacat atatttaca tcccgatcc gtttagtgt ccaaaacgg tttattgac accaacgct gaaatcgaa acgtccgca actgaaaga atatttcaa accgcaaat tgaagatat gtacagtaa attggtcta aaacgaaca agtattagg aattttctt agatctagg atgcaccaa agcaaattt ctcatttga agacttgtt tatagcaat atctatcga gtcaatcga gaacgcaat aaatacctt gggaggttg ccaaaacaa ttattttga tatttcgtt cggttttat caacaaata tgcattatc ttgcctttt agatatagg tttaacacc gtcaatctg actctctta ggttagaac attgtttat ctgacaaca attttattt attgttcgt tgttgtatt ctaaacaac cctttaata aagttattt gaaaaaa
