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Gene information
General information
TC006470 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10174801..10177159
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006470
    iBeetle screen iB_04150

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber slightly number decreased - Number of animals showing the phenotype: 4

    egg chamber shape rounded - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006470 RA
      MGGKFESLR VEYQDVLRK SFKTKTTDL IETKAKHLS 
      KSAIDLKAP LDQAFRDVL LELLEKEVN VSVLRELVD 
      FSVLTCRKE LSNPTMPVM LLSDIFDAL TLDACQEMF 
      LYVENNVEV WKENLFFSC CKNNLLRLC NDLLRRLSR 
      STATVFCGR ILLFLAKFF PFSERSGLN IVSEFNLEN 
      TTEYGTDSS QEKGGDVDG DEKKNVIDF TFYCKFWSL 
      QDFFRNPNQ LYTKVQWKL FCTYATSVF NTFQGLKLE 
      HVENNGSYC DESKNKMYF SKYLTSQKL LDLQLYDVN 
      FRRAVLLQF LILFQYLSS SVKFKSESF ELKSDQKEW 
      IQSSTEKVY SLLRETPPD GKRFAEIAS NILSREEYW 
      NAWKNDGCP ELKKSVVPT ESPDKRRTE ERPLLGDII 
      KQASSEGKY YMGSTELTK LWNLCPDNL EACKGRERD 
      FLPTLEDYF ADAIAQIES PGAVKDEDN LLKDGNYGW 
      RALRLLAKR SPHFFTYST VIMNPLSSY LEMMVKKIA 
      HDKPGRVTE NSQESQQND AEEENLFKD TQVDENTED 
      IKPSETEEF MDDKNARVE HKLNPNQLQ LFSEKVAPN 
      WKKLASKLG FKTDEIQFF SMENEKEVD QARNMLQLW 
      FDDDEDATL DNFLYILEG LEMEEAAEI VRNEINSMD 
      S
    CDS sequence
    • >TC006470 RA
      atggggggc aaattcgag tcgttacgg gtcgaatac 
      caggacgtt ttacgcaaa tctttcaag accaaaacc 
      accgatttg atcgaaact aaagccaaa cacttgtcg 
      aagtccgcc atcgacctt aaggcccct ctggaccag 
      gcgttccgc gacgtctta ctcgaactc cttgaaaag 
      gaagtcaac gtctcagtc ttacgcgag ttggtcgat 
      tttagcgtc ctaacatgt cgcaaggaa ctgagcaac 
      cccacaatg ccagtgatg ctcctttcg gacattttt 
      gacgcctta acgctggac gcctgtcag gaaatgttc 
      ctctacgta gagaacaat gtcgaagtg tggaaagag 
      aacttgttt ttcagttgt tgcaaaaat aatttactc 
      aggttgtgt aacgactta ttgaggcgg ttatctcgg 
      tccacggcg accgttttt tgcggccga attctcctc 
      tttttggcc aagtttttc ccgttttct gaacgttca 
      ggtttgaat attgtgagc gaatttaat ttggaaaac 
      acgacagag tatggcacg gactcgagc caggaaaag 
      gggggcgac gttgacggc gacgaaaag aaaaacgtg 
      atcgatttc acgttttat tgcaagttt tggtcgctc 
      caggatttt ttccggaac cccaaccag ttgtacact 
      aaagtgcaa tggaaactg ttttgcacc tacgccacg 
      agtgtcttt aacacgttc caaggcctc aagcttgaa 
      cacgtggaa aataacggg agctattgt gacgaaagt 
      aaaaataaa atgtacttt tccaagtac ctgacgagc 
      caaaaattg ctcgacttg caactgtac gacgtcaat 
      tttcggcgc gccgtcctg ttgcagttt ttaatttta 
      ttccagtac ctttcatca tccgtaaaa tttaaatcc 
      gaaagtttc gagttaaag tcggaccaa aaagagtgg 
      atccaatca agcaccgaa aaagtctac agtttgtta 
      cgcgagact cctccagac ggtaaacga tttgccgaa 
      atcgcctca aatattcta agtcgggag gagtattgg 
      aacgcttgg aagaacgac ggttgtccc gaactgaaa 
      aaatcggtg gttcccacg gaaagcccc gacaagcgc 
      cgaaccgaa gagcgccct ctcttaggc gacatcatt 
      aaacaggcc tccagtgag ggcaaatac tacatggga 
      agcaccgaa ctgaccaaa ctgtggaac ctatgcccc 
      gataacttg gaggcttgt aaaggccga gagcgcgat 
      tttctcccc acacttgag gattatttc gccgatgcc 
      atcgcccaa atcgagtca cctggcgca gtcaaagac 
      gaagacaat ttactgaaa gatgggaat tacgggtgg 
      cgggctttg agactcttg gctaagcgc agcccccac 
      tttttcacc tacagtact gtgattatg aaccccctg 
      tcttcgtat ttggagatg atggtcaag aaaatagcg 
      catgataag ccaggtcgc gtgaccgaa aattcgcaa 
      gagtcgcaa caaaacgac gccgaggaa gaaaatttg 
      tttaaggac acgcaagtt gatgaaaac accgaagac 
      atcaaaccg tccgaaacg gaggaattt atggacgat 
      aagaacgcg cgagtcgag cataaatta aacccgaac 
      cagttgcaa ctcttcagc gagaaagtg gcccccaat 
      tggaaaaaa ctagccagc aagttgggc ttcaaaact 
      gacgaaatc cagttcttc agtatggaa aatgagaaa 
      gaggtggac caggcccgt aatatgcta caactgtgg 
      ttcgatgac gacgaagat gccactttg gacaacttc 
      ttgtacatt ttggaaggg ttggagatg gaggaggcg 
      gcggaaatt gtccgaaat gaaatcaat tcgatggat 
      tcctaa
    mRNA sequence
    • >TC006470 RA
      tctaacatt atttttgac ctttgacag ttgtttaca 
      agacgattc cggtcgctt attcaagta tttcacacg 
      aaattgagc gtttttcac gttgggggc cctccaagc 
      gttgcattt aagcaatgg ggggcaaat tcgagtcgt 
      tacgggtcg aataccagg acgttttac gcaaatctt 
      tcaagacca aaaccaccg atttgatcg aaactaaag 
      ccaaacact tgtcgaagt ccgccatcg accttaagg 
      cccctctgg accaggcgt tccgcgacg tcttactcg 
      aactccttg aaaaggaag tcaacgtct cagtcttac 
      gcgagttgg tcgatttta gcgtcctaa catgtcgca 
      aggaactga gcaacccca caatgccag tgatgctcc 
      tttcggaca tttttgacg ccttaacgc tggacgcct 
      gtcaggaaa tgttcctct acgtagaga acaatgtcg 
      aagtgtgga aagagaact tgtttttca gttgttgca 
      aaaataatt tactcaggt tgtgtaacg acttattga 
      ggcggttat ctcggtcca cggcgaccg ttttttgcg 
      gccgaattc tcctctttt tggccaagt ttttcccgt 
      tttctgaac gttcaggtt tgaatattg tgagcgaat 
      ttaatttgg aaaacacga cagagtatg gcacggact 
      cgagccagg aaaaggggg gcgacgttg acggcgacg 
      aaaagaaaa acgtgatcg atttcacgt tttattgca 
      agttttggt cgctccagg attttttcc ggaacccca 
      accagttgt acactaaag tgcaatgga aactgtttt 
      gcacctacg ccacgagtg tctttaaca cgttccaag 
      gcctcaagc ttgaacacg tggaaaata acgggagct 
      attgtgacg aaagtaaaa ataaaatgt acttttcca 
      agtacctga cgagccaaa aattgctcg acttgcaac 
      tgtacgacg tcaattttc ggcgcgccg tcctgttgc 
      agtttttaa ttttattcc agtaccttt catcatccg 
      taaaattta aatccgaaa gtttcgagt taaagtcgg 
      accaaaaag agtggatcc aatcaagca ccgaaaaag 
      tctacagtt tgttacgcg agactcctc cagacggta 
      aacgatttg ccgaaatcg cctcaaata ttctaagtc 
      gggaggagt attggaacg cttggaaga acgacggtt 
      gtcccgaac tgaaaaaat cggtggttc ccacggaaa 
      gccccgaca agcgccgaa ccgaagagc gccctctct 
      taggcgaca tcattaaac aggcctcca gtgagggca 
      aatactaca tgggaagca ccgaactga ccaaactgt 
      ggaacctat gccccgata acttggagg cttgtaaag 
      gccgagagc gcgattttc tccccacac ttgaggatt 
      atttcgccg atgccatcg cccaaatcg agtcacctg 
      gcgcagtca aagacgaag acaatttac tgaaagatg 
      ggaattacg ggtggcggg ctttgagac tcttggcta 
      agcgcagcc cccactttt tcacctaca gtactgtga 
      ttatgaacc ccctgtctt cgtatttgg agatgatgg 
      tcaagaaaa tagcgcatg ataagccag gtcgcgtga 
      ccgaaaatt cgcaagagt cgcaacaaa acgacgccg 
      aggaagaaa atttgttta aggacacgc aagttgatg 
      aaaacaccg aagacatca aaccgtccg aaacggagg 
      aatttatgg acgataaga acgcgcgag tcgagcata 
      aattaaacc cgaaccagt tgcaactct tcagcgaga 
      aagtggccc ccaattgga aaaaactag ccagcaagt 
      tgggcttca aaactgacg aaatccagt tcttcagta 
      tggaaaatg agaaagagg tggaccagg cccgtaata 
      tgctacaac tgtggttcg atgacgacg aagatgcca 
      ctttggaca acttcttgt acattttgg aagggttgg 
      agatggagg aggcggcgg aaattgtcc gaaatgaaa 
      tcaattcga tggattcct aactgtatt taactcgtt 
      tcgccctat ttttacttt tcaatttgc gtttcatat 
      gtaattgtt tgtgatcaa ccttgggac agtcccgtt 
      ttttgtgcc ccggttgtt tacagtgga aacaatcct 
      ttaataaaa ttattaaac cttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY