| General information | |||
| TC034095
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9970371..9979136 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034095 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04148 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC034095 RA
MWQAPPPPR FQATKGGAS HVEAFERVV RHFASGRVA TSKTDTSST PLTNNLEDH SSEEDGSMA LLGRDKEMD KVAPQPLPP RYRLRDLIM GDYAFNDDG ERSCAILGG PAQIAGVQA SLIRNNSDG TFSLDELRD RIRKNPDCH EPYTTLVII ENTHNMCGG KVKLIIIKN PPLDWIEKV KNISKEYNI PVHMDGARV INAAVYLKV PVERVVRDV DTVCFCLSK GLGAPIGSI LAGSKTFVL GGGWRQAGI IAAAGLVAL DKMIDRLQE DHDHILQIA RAIDSLRSD IFKVDLPSI HTNILIGAK ASSIRPDCV RFVTYWQIT DDDIRDTID RVTFVLKGF DDQFKINFP RLHKHEITM EKEESQQAE VQTAEVTTC NEESVKKFC DADIAGQDS CESHPYKHL LNFEPTVEQ LSRVENIEA PKTLKETPL CDIDTKTSL EELITDLKA WKELGVDVE YSDHGYKAL TCLVQISTP DKDYIIDAI ALKDELHAL NEVFTNPDI VKIFHSAVN DLKWLQQDL GVFVVNLFD TQIAMKALG YKKLGLDAL LQDYHVAKD KTMQRQDFR RRPLPPKFK DYARVDSHY LIGFYHKLK NELIEANLL RAVLDDCNN CCKILYPKV EDEAYLSVR RNVEEIHKR QLLVLEKLN SWRHQIAQY LDKNVGCVL SKSNMETLV LQMPSDSAD IMQISQSSH VKEHLDEVM QILSEGNPV RFNEFSRNK RKFNQTGFG RNEPSGSNK KKWNNQIGK NVQNMFDLR NTLPQRDRK PFWNKQAAK RFHKYN
- >TC034095 RA
atgtggcaa gcgcctccg ccgccacgt ttccaagca actaaaggt ggcgcaagt catgtggag gccttcgaa cgcgtggtc cgccacttt gcatccggc cgcgtcgcg acgtccaag accgacacc tcgtccacg ccgctcacc aacaacctg gaggaccac tcctccgaa gaagacggg agtatggca ctcttggga cgagacaag gaaatggat aaagttgct cctcagcct ctaccaccg agatacaga ctgagggat cttatcatg ggggattac gcctttaac gacgatggc gaaaggagt tgcgcaatt ttagggggc cccgcccaa attgccggc gtccaagcc tcccttatc cgcaataac agtgacggg acgttcagt ttggacgaa ctcagagac agaatccgg aaaaatccg gactgtcat gagccctac accactcta gtcattatt gaaaacacc cacaacatg tgtggcgga aaagtaaag ttaatcata ataaaaaac ccaccgtta gattggatc gaaaaagtg aaaaacatt tcaaaagag tacaacatt ccggtccat atggacggg gctcgcgtc ataaacgcc gccgtctac ctcaaagta cctgtcgaa cgcgtcgtc cgggacgtg gacaccgtt tgcttctgc ctgagtaag gggctaggc gcccccatt gggtcgatt ctcgccggc agcaagact tttgtcctg gggggcggc tggcggcag gctgggatt atcgcagcc gcggggctc gtggccctt gacaagatg atcgaccgg ttgcaggag gaccacgac cacatctta caaattgcc agagctatc gatagcttg agatcggac atcttcaaa gttgacttg ccctcaatt cacacaaat attttgatc ggagcaaag gcttcctcc attcggccc gattgtgtc aggtttgtg acgtactgg cagatcacc gatgatgat atcagagat acgatcgac agagttacg tttgttttg aaaggattc gatgaccaa ttcaaaatt aacttccct cgcttacat aagcacgaa ataacaatg gaaaaagaa gaatcccaa caagctgaa gtccagact gctgaagta acgacatgt aatgaagaa tctgtgaaa aaattttgt gacgctgac attgctggc caggattcg tgtgaaagc cacccctac aagcacctt ctcaatttt gagcccacc gttgagcag ttgtctcgc gttgagaac atagaggcc cccaaaacg ctcaaggaa acccctctg tgtgacatc gacacgaaa accagtctc gaggaatta ataaccgat ttgaaagcc tggaaggag ttaggggtt gacgttgaa tactcggat catggctac aaagctttg acttgtctc gtccagata tcgacccct gacaaagac tacatcatc gatgcgatt gccttaaaa gacgagttg cacgccttg aatgaagtt ttcaccaat ccagacatt gtgaaaatt tttcattcg gcggtaaat gacctgaag tggctccag caggacttg ggtgttttt gtcgtcaac ttgtttgac actcaaatc gcgatgaaa gcgctgggg tacaaaaag ttgggactg gacgcactt ttgcaggac tatcacgtc gcaaaggat aagacaatg caacgtcag gattttcgc agaaggccc ctgcctccc aaatttaaa gactacgcc cgtgtggac tcccattac ttaatcggg ttttaccac aaactcaaa aatgagttg attgaagcg aaccttttg cgggctgtg cttgatgat tgtaataac tgctgcaag attctctat cctaaagtt gaagatgaa gcgtatttg tcggttcgt agaaatgtc gaagaaata cacaaacgc cagttgtta gttttggaa aaactgaac agctggagg caccaaatt gcccaatat ttggacaaa aacgtgggc tgtgtctta tctaagagt aatatggag accttggtt ttgcaaatg ccgagtgat tcggctgat attatgcaa atttcgcag tcatcacac gtgaaggaa catttggac gaagttatg caaatattg tcggaggga aatcccgtc cggtttaat gagttttct agaaataaa cgtaaattt aaccaaact gggtttggt agaaatgaa ccgtcggga agtaataag aaaaaatgg aataatcaa attggcaaa aatgtgcaa aacatgttt gacttgagg aacactttg ccccaaagg gacaggaag cccttctgg aacaagcaa gcggccaaa cgttttcac aagtataac tag
- >TC034095 RA
gtggaaaag ccgtccaaa gcgtccagc gtccggcat ctatctaat taaattgca tgtggcaag cgcctccgc cgccacgtt tccaagcaa ctaaaggtg gcgcaagtc atgtggagg ccttcgaac gcgtggtcc gccactttg catccggcc gcgtcgcga cgtccaaga ccgacacct cgtccacgc cgctcacca acaacctgg aggaccact cctccgaag aagacggga gtatggcac tcttgggac gagacaagg aaatggata aagttgctc ctcagcctc taccaccga gatacagac tgagggatc ttatcatgg gggattacg cctttaacg acgatggcg aaaggagtt gcgcaattt tagggggcc ccgcccaaa ttgccggcg tccaagcct cccttatcc gcaataaca gtgacggga cgttcagtt tggacgaac tcagagaca gaatccgga aaaatccgg actgtcatg agccctaca ccactctag tcattattg aaaacaccc acaacatgt gtggcggaa aagtaaagt taatcataa taaaaaacc caccgttag attggatcg aaaaagtga aaaacattt caaaagagt acaacattc cggtccata tggacgggg ctcgcgtca taaacgccg ccgtctacc tcaaagtac ctgtcgaac gcgtcgtcc gggacgtgg acaccgttt gcttctgcc tgagtaagg ggctaggcg cccccattg ggtcgattc tcgccggca gcaagactt ttgtcctgg ggggcggct ggcggcagg ctgggatta tcgcagccg cggggctcg tggcccttg acaagatga tcgaccggt tgcaggagg accacgacc acatcttac aaattgcca gagctatcg atagcttga gatcggaca tcttcaaag ttgacttgc cctcaattc acacaaata ttttgatcg gagcaaagg cttcctcca ttcggcccg attgtgtca ggtttgtga cgtactggc agatcaccg atgatgata tcagagata cgatcgaca gagttacgt ttgttttga aaggattcg atgaccaat tcaaaatta acttccctc gcttacata agcacgaaa taacaatgg aaaaagaag aatcccaac aagctgaag tccagactg ctgaagtaa cgacatgta atgaagaat ctgtgaaaa aattttgtg acgctgaca ttgctggcc aggattcgt gtgaaagcc acccctaca agcaccttc tcaattttg agcccaccg ttgagcagt tgtctcgcg ttgagaaca tagaggccc ccaaaacgc tcaaggaaa cccctctgt gtgacatcg acacgaaaa ccagtctcg aggaattaa taaccgatt tgaaagcct ggaaggagt taggggttg acgttgaat actcggatc atggctaca aagctttga cttgtctcg tccagatat cgacccctg acaaagact acatcatcg atgcgattg ccttaaaag acgagttgc acgccttga atgaagttt tcaccaatc cagacattg tgaaaattt ttcattcgg cggtaaatg acctgaagt ggctccagc aggacttgg gtgtttttg tcgtcaact tgtttgaca ctcaaatcg cgatgaaag cgctggggt acaaaaagt tgggactgg acgcacttt tgcaggact atcacgtcg caaaggata agacaatgc aacgtcagg attttcgca gaaggcccc tgcctccca aatttaaag actacgccc gtgtggact cccattact taatcgggt tttaccaca aactcaaaa atgagttga ttgaagcga accttttgc gggctgtgc ttgatgatt gtaataact gctgcaaga ttctctatc ctaaagttg aagatgaag cgtatttgt cggttcgta gaaatgtcg aagaaatac acaaacgcc agttgttag ttttggaaa aactgaaca gctggaggc accaaattg cccaatatt tggacaaaa acgtgggct gtgtcttat ctaagagta atatggaga ccttggttt tgcaaatgc cgagtgatt cggctgata ttatgcaaa tttcgcagt catcacacg tgaaggaac atttggacg aagttatgc aaatattgt cggagggaa atcccgtcc ggtttaatg agttttcta gaaataaac gtaaattta accaaactg ggtttggta gaaatgaac cgtcgggaa gtaataaga aaaaatgga ataatcaaa ttggcaaaa atgtgcaaa acatgtttg acttgagga acactttgc cccaaaggg acaggaagc ccttctgga acaagcaag cggccaaac gttttcaca agtataact agaccacaa atttttttt tttttggat taattcttt ttggtaagt ttcaatttt attcagtta attatgttt tttatgagt ggtttggac cacttttat ttttttcat ttgggtaca aatgcgtct ttatggaaa cgctacttc acaaaatac agcttaatg tgtttgaaa aaattagaa agatatttc gcttaactc cacaatgtg aattcataa gtgaaatat ttcgaaaac tactgtggg tatagggct cctgttgtt gataaaaac aaaactaaa acatttaag atattggaa aagacaata acaaacata ttgtataag tgcggcaat aaattatat agttcattc aaaaaaaaa aaaaataaa gtaacaaac ctttc
