Status
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Gene information
General information
TC006457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9906961..9930965
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006457
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06216

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    iBeetle screen iB_04147

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006457 RA
      MFRTRDSTS AMIIFSDSN DDMGIVFTL EGLTQAGII 
      FIMAIAIIV TNILIIATF LNFRGPSEV INCYLLSLA 
      VADLLCGLL VVPLSVYPA VVREWVYGD IVCRLVGYL 
      EVTLWAVTV YTFMWISVD RYLAVRKPL RYETLQTKT 
      RCQCWMAFT WISAAMLCC PPLLGFNQP VFDKDAYIC 
      MLDWGSMAA YSVTLSILV LGPSLITIV YTYTYIFNM 
      LRKLRSGYA FHDKEYATA LSENLSNPS HLMSFSLVV 
      AFWVSWTPY IGVKLYEYF TGVKLQVQF LHFGIVWLG 
      FLNSFWKSM ILITMSPQF RLALRIFCM TICCRYKGR 
      MQAELIGME ADD
    CDS sequence
    • >TC006457 RA
      atgttccgc accagggac agcacctcg gctatgatc 
      atcttcagc gactccaac gatgacatg ggaatcgtg 
      ttcacgctg gagggtctc acccaagcg ggcatcatc 
      ttcatcatg gccattgcc atcatcgtc accaacata 
      ctcatcatc gcaaccttc ctcaatttc agaggtcca 
      tcggaggtg atcaactgc tacttgctg tctctggcc 
      gtagccgac ctgctgtgc ggcctgctg gtggtgccc 
      ctgtccgtg tacccggcc gtggtgcgc gagtgggtc 
      tacggggac atcgtgtgc cggctggtg ggctacctg 
      gaggtgacc ctgtgggcg gtgacagtg tacacgttc 
      atgtggata agcgtggac cgctacttg gccgtccgc 
      aagcccctc cgctacgag accctccag accaagacg 
      cggtgccag tgctggatg gccttcacc tggatctcg 
      gcggcgatg ctgtgctgc ccccctctg ctgggcttc 
      aaccagccc gtgttcgac aaggacgcc tacatctgc 
      atgctggat tggggcagc atggcggcc tactcggtc 
      acgctgtcc atcctggtg ctgggcccc agcctcatc 
      accattgtc tacacctac acctacatc ttcaacatg 
      ctgcggaag ctgcgcagc ggctacgcc ttccacgac 
      aaggagtac gccacagca ctgtcggag aacctctcc 
      aaccccagc catctgatg tcgttctcg ctggtggtg 
      gcgttctgg gtgtcctgg acgccctac atcggggtc 
      aagctgtac gagtacttc accggtgtt aagttacaa 
      gtgcaattt ttacatttc gggatcgtc tggttgggc 
      ttcttgaac tcgttctgg aagtcgatg attctgatc 
      acgatgagt ccgcagttc aggctggcg ctgaggatc 
      ttctgcatg acgatctgc tgcaggtac aagggccga 
      atgcaggcc gagctgata gggatggaa gcagatgac 
      tga
    mRNA sequence
    • >TC006457 RA
      gctcagcta agcctggaa catcagttc tcggtcaga 
      tcgcaaagt gtgagcttt tcggtcaat gctgtcatg 
      ttctgtggc ggcactcaa cttccggat tattgcgcg 
      gtttcaatg ctcatcttg cgcttctag gtgtgtatt 
      ttaagcgga aaaacggtc aggtttagg ttgcttcaa 
      ggaagggaa agtttagcg cggattatg agatgtgcg 
      atgttttca ggagctgtg atgcttgac ggttccaca 
      ttgctgttc ttcaatgac tgaagacga ctttaagaa 
      cgtccatgc gaccgcgct gtgagcagg ccaggtgat 
      ccttaacga gatggcgta taggtggcg ccacctgca 
      tccgggtcc gcttctcct ccggcatcg ctagcggcc 
      cggagccgg aatgttccg caccaggga cagcacctc 
      ggctatgat catcttcag cgactccaa cgatgacat 
      gggaatcgt gttcacgct ggagggtct cacccaagc 
      gggcatcat cttcatcat ggccattgc catcatcgt 
      caccaacat actcatcat cgcaacctt cctcaattt 
      cagaggtcc atcggaggt gatcaactg ctacttgct 
      gtctctggc cgtagccga cctgctgtg cggcctgct 
      ggtggtgcc cctgtccgt gtacccggc cgtggtgcg 
      cgagtgggt ctacgggga catcgtgtg ccggctggt 
      gggctacct ggaggtgac cctgtgggc ggtgacagt 
      gtacacgtt catgtggat aagcgtgga ccgctactt 
      ggccgtccg caagcccct ccgctacga gaccctcca 
      gaccaagac gcggtgcca gtgctggat ggccttcac 
      ctggatctc ggcggcgat gctgtgctg cccccctct 
      gctgggctt caaccagcc cgtgttcga caaggacgc 
      ctacatctg catgctgga ttggggcag catggcggc 
      ctactcggt cacgctgtc catcctggt gctgggccc 
      cagcctcat caccattgt ctacaccta cacctacat 
      cttcaacat gctgcggaa gctgcgcag cggctacgc 
      cttccacga caaggagta cgccacagc actgtcgga 
      gaacctctc caaccccag ccatctgat gtcgttctc 
      gctggtggt ggcgttctg ggtgtcctg gacgcccta 
      catcggggt caagctgta cgagtactt caccggtgt 
      taagttaca agtgcaatt tttacattt cgggatcgt 
      ctggttggg cttcttgaa ctcgttctg gaagtcgat 
      gattctgat cacgatgag tccgcagtt caggctggc 
      gctgaggat cttctgcat gacgatctg ctgcaggta 
      caagggccg aatgcaggc cgagctgat agggatgga 
      agcagatga ctgactact ctttcccgc caccggatg 
      ttttccggg tttccccat cacaggact cgcaatgcg 
      agactatga gtacttgct ggataaaat caggcggta 
      ctgctggca ctactccag acgcaatca attttacaa 
      ccagtgact cgggacttg atcgatgtg gctgtagat 
      actttgtac agatttcaa gtgttttgt attttaaca 
      ttaacagtc aattaatat tacttgtag gttttaatc 
      tccgagatt aaggagctc caggaggta gacaagttt 
      tttcgcact tatttgccc actctggaa acgtgcata 
      gaattattt gcttagttg gattcggga aatattaat 
      gctttattc acaaagttc aaacttttg tggaaactg 
      cgttagacc atcatatca agtctgtaa gtggactat 
      taactttgt gattaaaca gtctttaaa ttgtctttt 
      tcaattact ccaaatctc tacaaatac accaacagt 
      ttacataaa aaacggaaa taaaaattt ttacagtgt 
      agataatat tccacgtct tggccactg aaaaagtcg 
      cataccaaa gtaatgaaa aaatctaaa aacaagaca 
      aagatgctt ttttaacaa aaacacatc aaaaaataa 
      ataaataaa ataaaaaag gcaaaaaat atcttgata 
      ataatacat aaaaggctt cacctttcc atttttgcc 
      ttatttgat agaagataa tagtttttt gcttatttt 
      gaaaaatca cagtaacta taatatgaa tttagtgtt 
      actttaaac tcactctgt atgtccaaa aatgcagaa 
      atatagggt gttttattt aaaaaatat atacatttg 
      ttttgtaac attttttga atcaaaaat ttttgtaca 
      aatttattt aatataata tatatatat tatatataa 
      ttatatata ttatatata taatataat ataatagaa 
      tattagaaa atagaaaat atacacaac aaacatcct 
      aaaacaaaa taataaact aattagtta ggcagagaa 
      ttgacttac gaaaataat gtaaattga agatttaag 
      aaagtaact ttctccttt caagttatg atggcttta 
      agacttttt cttgcagac aatggtttc tgtatactt 
      accttattt cctgaagaa ttattgctt attaacttt 
      ctcattcat ctgccaagg taaaaacta ttatataaa 
      aaaattctg aaaatcaaa gaaacaatt ctttatttt 
      tgcagattt cgaggcaca tttgcattt caaatgtaa 
      tttcaaatt caaaagatt cataattgg aaaaacaag 
      ttaaatttt tttgccaga atttatcga tcaccaact 
      accaaaaca attccatcc atgattgct gagtctggg 
      ccgaaaaaa ccgctacct gctgggcaa aatgcagct 
      tatttggga agctcataa cgattttac aacccccgt 
      gtattggct gcttatgtg taatcaaaa atgaggctt 
      aacaccaac ttttaacga ccgttgttc aaactttat 
      cgagatgca gacccaaac aatgttgga ggacgatag 
      ttataatca gggtaacca gctgcaatt gaagcattt 
      ttaggcctg catctgctt gatccgcaa acacaatca 
      aactttttc attactaga agtgcaacg aagcaaaac 
      ccaaaatta tacaccgat tattaaatt ggacgtgat 
      gattttaat gtagttgtt aaggatgag cttaaaatt 
      gagcaatca aaacgtatt gctagatca cacataata 
      agttgcctt ggattttgt actgatcat ccctttgaa 
      agatttacg tcgacactg tgttaggtc taatgtttc 
      tcaggctaa atagacttc atgttttta gagcacagt 
      ttctaatat tacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY