| General information | |||
| TC006456 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9900561..9905432 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006456 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04146 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC006456 RA
MPRYLLVLV VLFIEVYSS PIKHKAVSS CQACHGVDP NKINVHLIP HSHDDVGWL KTVDQYYYG SHSEIQKAG VQYIISSTV EALKADPDR RFVQVETAF FWKWWQHQP DTIRQDFID LVNSGQIEI INAAWSMND EAAVNYHST IDQFTYVFR IINDTIGKC GIPRIGWQI DPFGHSREQ ASIFAQLGY DGVFFARID YTDRDQRIA DKNMEVIWR GSANSENIN IFTNVFPEF YYPPAGYCF DIECGDEVI NDDTKSPDY NVPQRVEEF QKKVESLAA FYQTSNLLI PMGGDFQYQ SAEKNFINM DKLIAGFKG NDKINIIYS TPSCYIKAV NDEATSKNI KFTLKTDDF FPYGSASHC YWTGYFTSR PNAKRFERT ANNILQAGK QLASFSKVK GTDYDDDLT LLKQAVGIL QHHDAITGT AKEAVANDY ARLLNKGIK KAEPSLNVI VTNLLKKDA SSDINLNLE TCLLSNVSI CEITKSERF IVSIYNPLE RPVTHYVRV PVPDGTYKI TGPDGEVTV DIVDSISSF EYIDTNFGT PSPKELVFA ANDVPGFGI RLYYVEKTS AKSRPVKQS PKVKFGTDT TGFEIDEKT GLLKSVTMN GQTVEITQQ FLYYNGFNG DNNGDDKQA SGAYIFRPK ENEATVVSD SVTVTSTTG TLVDEVRQQ VNDWITQII RVYKGENDN YIEFDWLIG QIEVDNDNG IGREIVSRF TVKDFDNGE SFFTDSNGR ELIKRQLNK RYDYEYDST LEPIASNYY PVTSKIVIR DETKKLQVA VLNDRAQGG ASLQNGALE LMLHRRLLA DDNKGVGEP LDDEEFGQG VVARGQLYL VVGSTDSNV SGGKSTAAQ ERELALKKL LNPLVLVGD ASSDALSLD KVQSALNFN FEGLKTTLP DNVHILTLE PWKDDSYIL RLEHILENN EDATLSTTA TVNLEGLFT LFEITEIRE TTLGANQWY DEYAAEDKY IWALKDGSS VSNKEAPVT VTADLQITL DPMQIRTFI IKVK
- >TC006456 RB
MPRYLLVLV VLFIEVYSS PIKHKAVSS CQACHGVDP NKINVHLIP HSHDDVGWL KTVDQYYYG SHSEIQKAG VQYIISSTV EALKADPDR RFVQVETAF FWKWWQHQP DTIRQDFID LVNSGQIEI INAAWSMND EAAVNYHST IDQFTYGLK IINDTIGKC GIPRIGWQI DPFGHSREQ ASIFAQLGY DGVFFARID YTDRDQRIA DKNMEVIWR GSANSENIN IFTNVFPEF YYPPAGYCF DIECGDEVI NDDTKSPDY NVPQRVEEF QKKVESLAA FYQTSNLLI PMGGDFQYQ SAEKNFINM DKLIAGFKG NDKINIIYS TPSCYIKAV NDEATSKNI KFTLKTDDF FPYGSASHC YWTGYFTSR PNAKRFERT ANNILQAGK QLASFSKVK GTDYDDDLT LLKQAVGIL QHHDAITGT AKEAVANDY ARLLNKGIK KAEPSLNVI VTNLLKKDA SSDINLNLE TCLLSNVSI CEITKSERF IVSIYNPLE RPVTHYVRV PVPDGTYKI TGPDGEVTV DIVDSISSF EYIDTNFGT PSPKELVFA ANDVPGFGI RLYYVEKTS AKSRPVKQS PKVKFGTDT TGFEIDEKT GLLKSVTMN GQTVEITQQ FLYYNGFNG DNNGDDKQA SGAYIFRPK ENEATVVSD SVTVTSTTG TLVDEVRQQ VNDWITQII RVYKGENDN YIEFDWLIG QIEVDNDNG IGREIVSRF TVKDFDNGE SFFTDSNGR ELIKRQLNK RYDYEYDST LEPIASNYY PVTSKIVIR DETKKLQVA VLNDRAQGG ASLQNGALE LMLHRRLLA DDNKGVGEP LDDEEFGQG VVARGQLYL VVGSTDSNV SGGKSTAAQ ERELALKKL LNPLVLVGD ASSDALSLD KVQSALNFN FEGLKTTLP DNVHILTLE PWKDDSYIL RLEHILENN EDATLSTTA TVNLEGLFT LFEITEIRE TTLGANQWY DEYAAEDKY IWALKDGSS VSNKEAPVT VTADLQITL DPMQIRTFI IKVK
- >TC006456 RA
atgccgaga tatttactg gtgttggtg gtgcttttc attgaggtg tattcatcg ccaatcaaa cacaaagcg gtgtcaagc tgccaagcc tgccatggc gtagatccc aacaaaatt aatgttcat ctcatccct cattcacac gatgacgtc ggatggttg aaaactgtt gatcaatac tactacgga agtcacagc gagatacaa aaggccgga gtacaatac attatttca tcaactgtt gaagcactc aaagcggat ccggatagg agatttgtt caggtggaa acagcgttt ttctggaag tggtggcaa caccagccc gataccatc agacaagat ttcattgat ttggtcaac agtggacaa attgaaatt atcaatgcg gcctggtct atgaatgat gaagcggct gttaattat cactcgacg attgatcag ttcacctat gtttttaga ataattaat gacactatt ggaaaatgt ggaatcccg agaatcggc tggcaaatt gatccgttc ggtcatagt cgggaacaa gcctcaatt tttgcacag ttgggctat gatggtgtt ttctttgcc agaattgat tacactgat cgggatcaa agaattgcc gacaaaaat atggaagtt atttggaga ggcagtgcc aactctgag aatatcaat atttttact aacgttttt cccgaattt tattatcct cccgctggt tattgcttc gatattgag tgtggagat gaggttatt aatgacgat actaaaagt cctgactat aatgttcca caacgtgtt gaagaattc caaaagaaa gtcgagtca ttggctgca ttctaccaa actagcaat cttctgatc cccatggga ggagacttc cagtatcag tcagcagag aagaacttt atcaacatg gataaactt atagctggt tttaagggc aatgacaaa attaacata atttactca accccgtct tgctacatt aaagcagtg aatgatgag gccactagc aaaaatatc aaattcact ttaaaaact gacgatttc ttcccatac ggctctgct tcacactgt tactggact ggatacttt acttcacgt cccaatgcc aaacgattt gaaagaaca gccaacaac attttacaa gctgggaaa caattggca tctttctca aaagtcaaa ggaactgat tacgacgac gatttaaca cttttgaag caagctgtt ggtattttg caacaccat gacgccatc actggcacc gccaaagaa gcagtggct aatgactat gccagactt ttgaacaaa ggtattaag aaagctgaa ccctcattg aacgttata gtcacaaac cttctcaaa aaagatgcg tcttctgat atcaatctc aacttggag acttgcttg ttgtcaaat gttagtatt tgtgagatt accaaatct gagcgattt attgtatca atttacaac cctcttgaa cgccctgtt acacattac gtacgtgtg cctgtacca gatggtact tacaaaatc acaggacct gatggtgaa gtaactgtt gatatcgtt gacagtata tccagtttt gaatacatt gatacaaat ttcggaact ccaagtccc aaagaactg gtctttgcc gctaatgat gttcccggt tttgggatc agactgtat tatgttgaa aaaacttct gcaaaatcg cgtccagtg aaacaatca cccaaagtt aaattcggc actgataca actgggttt gaaattgat gaaaaaacc ggtcttttg aaatctgtt accatgaac ggacaaact gtggaaatc actcaacaa ttcttatat tataacggt tttaatgga gacaataat ggcgatgat aaacaagcg tctggagct tacattttc cgacccaag gaaaatgaa gctacagtt gttagtgat tcagtgact gttacttca acgactgga actctggtc gatgaagtc cgtcaacaa gtgaacgac tggattacc caaattatc agggtttat aaaggagaa aacgacaat tacatcgag tttgattgg ctcattggt cagatagaa gtggacaac gacaatggg attgggcgc gaaattgtc agccgattt acagtcaaa gacttcgac aatggtgaa tccttcttt accgactcc aacggccgt gaattaatc aaaagacaa ctcaacaaa cgatatgat tacgaatat gactcgacc cttgaacca attgccagt aactactac cctgtgact tcaaaaatt gtaattcgg gacgaaacc aaaaaattg caagttgca gttttgaac gatcgagct caaggaggt gcaagtttg caaaacgga gcgcttgaa cttatgctt cacagacga cttttggcc gatgataac aaaggtgta ggcgaacca cttgatgat gaagaattt gggcaaggt gtagttgca cgtggacag ctttacttg gttgttggt tcaactgat agtaatgtg agtggaggg aaatcaaca gcagcgcaa gaacgagaa ttggctttg aagaaattg ttgaatccg cttgtactt gttggtgat gcttcttcc gatgctttg tcgttagat aaagttcaa agtgctctc aactttaat tttgaaggt ttgaaaacc actcttcca gacaatgtt catattttg actcttgag ccgtggaaa gatgatagt tatatttta aggctggaa catattctg gagaataat gaagacgca acattgtca actacagca accgtgaat ttggaaggt ctatttaca ctttttgaa ataaccgaa attagggaa actacactt ggtgctaat caatggtat gatgaatat gcagctgaa gataagtac atctgggcg cttaaggat ggcagttct gtgtctaat aaagaagct ccagttaca gtaactgca gatttgcaa attacctta gatcctatg caaattcgt accttcata attaaggtt aaataa
- >TC006456 RB
atgccgaga tatttactg gtgttggtg gtgcttttc attgaggtg tattcatcg ccaatcaaa cacaaagcg gtgtcaagc tgccaagcc tgccatggc gtagatccc aacaaaatt aatgttcat ctcatccct cattcacac gatgacgtc ggatggttg aaaactgtt gatcaatac tactacgga agtcacagc gagatacaa aaggccgga gtacaatac attatttca tcaactgtt gaagcactc aaagcggat ccggatagg agatttgtt caggtggaa acagcgttt ttctggaag tggtggcaa caccagccc gataccatc agacaagat ttcattgat ttggtcaac agtggacaa attgaaatt atcaatgcg gcctggtct atgaatgat gaagcggct gttaattat cactcgacg attgatcag ttcacctat ggtcttaaa ataattaat gacactatt ggaaaatgt ggaatcccg agaatcggc tggcaaatt gatccgttc ggtcatagt cgggaacaa gcctcaatt tttgcacag ttgggctat gatggtgtt ttctttgcc agaattgat tacactgat cgggatcaa agaattgcc gacaaaaat atggaagtt atttggaga ggcagtgcc aactctgag aatatcaat atttttact aacgttttt cccgaattt tattatcct cccgctggt tattgcttc gatattgag tgtggagat gaggttatt aatgacgat actaaaagt cctgactat aatgttcca caacgtgtt gaagaattc caaaagaaa gtcgagtca ttggctgca ttctaccaa actagcaat cttctgatc cccatggga ggagacttc cagtatcag tcagcagag aagaacttt atcaacatg gataaactt atagctggt tttaagggc aatgacaaa attaacata atttactca accccgtct tgctacatt aaagcagtg aatgatgag gccactagc aaaaatatc aaattcact ttaaaaact gacgatttc ttcccatac ggctctgct tcacactgt tactggact ggatacttt acttcacgt cccaatgcc aaacgattt gaaagaaca gccaacaac attttacaa gctgggaaa caattggca tctttctca aaagtcaaa ggaactgat tacgacgac gatttaaca cttttgaag caagctgtt ggtattttg caacaccat gacgccatc actggcacc gccaaagaa gcagtggct aatgactat gccagactt ttgaacaaa ggtattaag aaagctgaa ccctcattg aacgttata gtcacaaac cttctcaaa aaagatgcg tcttctgat atcaatctc aacttggag acttgcttg ttgtcaaat gttagtatt tgtgagatt accaaatct gagcgattt attgtatca atttacaac cctcttgaa cgccctgtt acacattac gtacgtgtg cctgtacca gatggtact tacaaaatc acaggacct gatggtgaa gtaactgtt gatatcgtt gacagtata tccagtttt gaatacatt gatacaaat ttcggaact ccaagtccc aaagaactg gtctttgcc gctaatgat gttcccggt tttgggatc agactgtat tatgttgaa aaaacttct gcaaaatcg cgtccagtg aaacaatca cccaaagtt aaattcggc actgataca actgggttt gaaattgat gaaaaaacc ggtcttttg aaatctgtt accatgaac ggacaaact gtggaaatc actcaacaa ttcttatat tataacggt tttaatgga gacaataat ggcgatgat aaacaagcg tctggagct tacattttc cgacccaag gaaaatgaa gctacagtt gttagtgat tcagtgact gttacttca acgactgga actctggtc gatgaagtc cgtcaacaa gtgaacgac tggattacc caaattatc agggtttat aaaggagaa aacgacaat tacatcgag tttgattgg ctcattggt cagatagaa gtggacaac gacaatggg attgggcgc gaaattgtc agccgattt acagtcaaa gacttcgac aatggtgaa tccttcttt accgactcc aacggccgt gaattaatc aaaagacaa ctcaacaaa cgatatgat tacgaatat gactcgacc cttgaacca attgccagt aactactac cctgtgact tcaaaaatt gtaattcgg gacgaaacc aaaaaattg caagttgca gttttgaac gatcgagct caaggaggt gcaagtttg caaaacgga gcgcttgaa cttatgctt cacagacga cttttggcc gatgataac aaaggtgta ggcgaacca cttgatgat gaagaattt gggcaaggt gtagttgca cgtggacag ctttacttg gttgttggt tcaactgat agtaatgtg agtggaggg aaatcaaca gcagcgcaa gaacgagaa ttggctttg aagaaattg ttgaatccg cttgtactt gttggtgat gcttcttcc gatgctttg tcgttagat aaagttcaa agtgctctc aactttaat tttgaaggt ttgaaaacc actcttcca gacaatgtt catattttg actcttgag ccgtggaaa gatgatagt tatatttta aggctggaa catattctg gagaataat gaagacgca acattgtca actacagca accgtgaat ttggaaggt ctatttaca ctttttgaa ataaccgaa attagggaa actacactt ggtgctaat caatggtat gatgaatat gcagctgaa gataagtac atctgggcg cttaaggat ggcagttct gtgtctaat aaagaagct ccagttaca gtaactgca gatttgcaa attacctta gatcctatg caaattcgt accttcata attaaggtt aaataa
- >TC006456 RA
tcaatgaaa taaaaaatg ccgagatat ttactggtg ttggtggtg cttttcatt gaggtgtat tcatcgcca atcaaacac aaagcggtg tcaagctgc caagcctgc catggcgta gatcccaac aaaattaat gttcatctc atccctcat tcacacgat gacgtcgga tggttgaaa actgttgat caatactac tacggaagt cacagcgag atacaaaag gccggagta caatacatt atttcatca actgttgaa gcactcaaa gcggatccg gataggaga tttgttcag gtggaaaca gcgtttttc tggaagtgg tggcaacac cagcccgat accatcaga caagatttc attgatttg gtcaacagt ggacaaatt gaaattatc aatgcggcc tggtctatg aatgatgaa gcggctgtt aattatcac tcgacgatt gatcagttc acctatgtt tttagaata attaatgac actattgga aaatgtgga atcccgaga atcggctgg caaattgat ccgttcggt catagtcgg gaacaagcc tcaattttt gcacagttg ggctatgat ggtgttttc tttgccaga attgattac actgatcgg gatcaaaga attgccgac aaaaatatg gaagttatt tggagaggc agtgccaac tctgagaat atcaatatt tttactaac gtttttccc gaattttat tatcctccc gctggttat tgcttcgat attgagtgt ggagatgag gttattaat gacgatact aaaagtcct gactataat gttccacaa cgtgttgaa gaattccaa aagaaagtc gagtcattg gctgcattc taccaaact agcaatctt ctgatcccc atgggagga gacttccag tatcagtca gcagagaag aactttatc aacatggat aaacttata gctggtttt aagggcaat gacaaaatt aacataatt tactcaacc ccgtcttgc tacattaaa gcagtgaat gatgaggcc actagcaaa aatatcaaa ttcacttta aaaactgac gatttcttc ccatacggc tctgcttca cactgttac tggactgga tactttact tcacgtccc aatgccaaa cgatttgaa agaacagcc aacaacatt ttacaagct gggaaacaa ttggcatct ttctcaaaa gtcaaagga actgattac gacgacgat ttaacactt ttgaagcaa gctgttggt attttgcaa caccatgac gccatcact ggcaccgcc aaagaagca gtggctaat gactatgcc agacttttg aacaaaggt attaagaaa gctgaaccc tcattgaac gttatagtc acaaacctt ctcaaaaaa gatgcgtct tctgatatc aatctcaac ttggagact tgcttgttg tcaaatgtt agtatttgt gagattacc aaatctgag cgatttatt gtatcaatt tacaaccct cttgaacgc cctgttaca cattacgta cgtgtgcct gtaccagat ggtacttac aaaatcaca ggacctgat ggtgaagta actgttgat atcgttgac agtatatcc agttttgaa tacattgat acaaatttc ggaactcca agtcccaaa gaactggtc tttgccgct aatgatgtt cccggtttt gggatcaga ctgtattat gttgaaaaa acttctgca aaatcgcgt ccagtgaaa caatcaccc aaagttaaa ttcggcact gatacaact gggtttgaa attgatgaa aaaaccggt cttttgaaa tctgttacc atgaacgga caaactgtg gaaatcact caacaattc ttatattat aacggtttt aatggagac aataatggc gatgataaa caagcgtct ggagcttac attttccga cccaaggaa aatgaagct acagttgtt agtgattca gtgactgtt acttcaacg actggaact ctggtcgat gaagtccgt caacaagtg aacgactgg attacccaa attatcagg gtttataaa ggagaaaac gacaattac atcgagttt gattggctc attggtcag atagaagtg gacaacgac aatgggatt gggcgcgaa attgtcagc cgatttaca gtcaaagac ttcgacaat ggtgaatcc ttctttacc gactccaac ggccgtgaa ttaatcaaa agacaactc aacaaacga tatgattac gaatatgac tcgaccctt gaaccaatt gccagtaac tactaccct gtgacttca aaaattgta attcgggac gaaaccaaa aaattgcaa gttgcagtt ttgaacgat cgagctcaa ggaggtgca agtttgcaa aacggagcg cttgaactt atgcttcac agacgactt ttggccgat gataacaaa ggtgtaggc gaaccactt gatgatgaa gaatttggg caaggtgta gttgcacgt ggacagctt tacttggtt gttggttca actgatagt aatgtgagt ggagggaaa tcaacagca gcgcaagaa cgagaattg gctttgaag aaattgttg aatccgctt gtacttgtt ggtgatgct tcttccgat gctttgtcg ttagataaa gttcaaagt gctctcaac tttaatttt gaaggtttg aaaaccact cttccagac aatgttcat attttgact cttgagccg tggaaagat gatagttat attttaagg ctggaacat attctggag aataatgaa gacgcaaca ttgtcaact acagcaacc gtgaatttg gaaggtcta tttacactt tttgaaata accgaaatt agggaaact acacttggt gctaatcaa tggtatgat gaatatgca gctgaagat aagtacatc tgggcgctt aaggatggc agttctgtg tctaataaa gaagctcca gttacagta actgcagat ttgcaaatt accttagat cctatgcaa attcgtacc ttcataatt aaggttaaa taataaacg attgtttag agataagaa aataaagct tttgaaaaa ta
- >TC006456 RB
tcaatgaaa taaaaaatg ccgagatat ttactggtg ttggtggtg cttttcatt gaggtgtat tcatcgcca atcaaacac aaagcggtg tcaagctgc caagcctgc catggcgta gatcccaac aaaattaat gttcatctc atccctcat tcacacgat gacgtcgga tggttgaaa actgttgat caatactac tacggaagt cacagcgag atacaaaag gccggagta caatacatt atttcatca actgttgaa gcactcaaa gcggatccg gataggaga tttgttcag gtggaaaca gcgtttttc tggaagtgg tggcaacac cagcccgat accatcaga caagatttc attgatttg gtcaacagt ggacaaatt gaaattatc aatgcggcc tggtctatg aatgatgaa gcggctgtt aattatcac tcgacgatt gatcagttc acctatggt cttaaaata attaatgac actattgga aaatgtgga atcccgaga atcggctgg caaattgat ccgttcggt catagtcgg gaacaagcc tcaattttt gcacagttg ggctatgat ggtgttttc tttgccaga attgattac actgatcgg gatcaaaga attgccgac aaaaatatg gaagttatt tggagaggc agtgccaac tctgagaat atcaatatt tttactaac gtttttccc gaattttat tatcctccc gctggttat tgcttcgat attgagtgt ggagatgag gttattaat gacgatact aaaagtcct gactataat gttccacaa cgtgttgaa gaattccaa aagaaagtc gagtcattg gctgcattc taccaaact agcaatctt ctgatcccc atgggagga gacttccag tatcagtca gcagagaag aactttatc aacatggat aaacttata gctggtttt aagggcaat gacaaaatt aacataatt tactcaacc ccgtcttgc tacattaaa gcagtgaat gatgaggcc actagcaaa aatatcaaa ttcacttta aaaactgac gatttcttc ccatacggc tctgcttca cactgttac tggactgga tactttact tcacgtccc aatgccaaa cgatttgaa agaacagcc aacaacatt ttacaagct gggaaacaa ttggcatct ttctcaaaa gtcaaagga actgattac gacgacgat ttaacactt ttgaagcaa gctgttggt attttgcaa caccatgac gccatcact ggcaccgcc aaagaagca gtggctaat gactatgcc agacttttg aacaaaggt attaagaaa gctgaaccc tcattgaac gttatagtc acaaacctt ctcaaaaaa gatgcgtct tctgatatc aatctcaac ttggagact tgcttgttg tcaaatgtt agtatttgt gagattacc aaatctgag cgatttatt gtatcaatt tacaaccct cttgaacgc cctgttaca cattacgta cgtgtgcct gtaccagat ggtacttac aaaatcaca ggacctgat ggtgaagta actgttgat atcgttgac agtatatcc agttttgaa tacattgat acaaatttc ggaactcca agtcccaaa gaactggtc tttgccgct aatgatgtt cccggtttt gggatcaga ctgtattat gttgaaaaa acttctgca aaatcgcgt ccagtgaaa caatcaccc aaagttaaa ttcggcact gatacaact gggtttgaa attgatgaa aaaaccggt cttttgaaa tctgttacc atgaacgga caaactgtg gaaatcact caacaattc ttatattat aacggtttt aatggagac aataatggc gatgataaa caagcgtct ggagcttac attttccga cccaaggaa aatgaagct acagttgtt agtgattca gtgactgtt acttcaacg actggaact ctggtcgat gaagtccgt caacaagtg aacgactgg attacccaa attatcagg gtttataaa ggagaaaac gacaattac atcgagttt gattggctc attggtcag atagaagtg gacaacgac aatgggatt gggcgcgaa attgtcagc cgatttaca gtcaaagac ttcgacaat ggtgaatcc ttctttacc gactccaac ggccgtgaa ttaatcaaa agacaactc aacaaacga tatgattac gaatatgac tcgaccctt gaaccaatt gccagtaac tactaccct gtgacttca aaaattgta attcgggac gaaaccaaa aaattgcaa gttgcagtt ttgaacgat cgagctcaa ggaggtgca agtttgcaa aacggagcg cttgaactt atgcttcac agacgactt ttggccgat gataacaaa ggtgtaggc gaaccactt gatgatgaa gaatttggg caaggtgta gttgcacgt ggacagctt tacttggtt gttggttca actgatagt aatgtgagt ggagggaaa tcaacagca gcgcaagaa cgagaattg gctttgaag aaattgttg aatccgctt gtacttgtt ggtgatgct tcttccgat gctttgtcg ttagataaa gttcaaagt gctctcaac tttaatttt gaaggtttg aaaaccact cttccagac aatgttcat attttgact cttgagccg tggaaagat gatagttat attttaagg ctggaacat attctggag aataatgaa gacgcaaca ttgtcaact acagcaacc gtgaatttg gaaggtcta tttacactt tttgaaata accgaaatt agggaaact acacttggt gctaatcaa tggtatgat gaatatgca gctgaagat aagtacatc tgggcgctt aaggatggc agttctgtg tctaataaa gaagctcca gttacagta actgcagat ttgcaaatt accttagat cctatgcaa attcgtacc ttcataatt aaggttaaa taataaacg attgtttag agataagaa aataaagct tttgaaaaa ta
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