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Gene information
General information
TC006453 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9872071..9880143
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006453
    iBeetle screen iB_04145

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006453 RA
      MFAKFLKIA LFINLVLCK PPKRLSDTI PATCHACHP 
      IAPEKINIH LVPHSHDDV GWVKTTEQY YYGSKKKVQ 
      FAGVQYIIS STIEALKRN PKRRFIQVE SVFFSKWWQ 
      NQDETLRQE VIEMVKRGQ LEIVNGAWT MNDEADTNY 
      QSIIDQFTW GFRFLDDNL GKCARPKVG WQIDSFGHS 
      REHASILTQ MGFQGLVIG RLDHRDKEK RQQEKNLDF 
      VWQASANLN NSAIFTTMF PEFYVSIEC FCFDILCDD 
      EMINDDVKS PEFNLNEKV YMFSQAMDQ FKEYYKTNN 
      ILLPMGGDF TYQAAEINF SNIDKLIKG FQGHKKYNV 
      FYSTPSCYI AAVNEEVNR KGIKLRTKT DDFFPYSLT 
      RDAFWTGYY TSRPTLKRF ERVGNNILQ AVKQLTTFS 
      RIKGKDKSY FIDNLRGAM GIMQHHDAI TGTEREAVA 
      KDYVKMLNK AIREAETPI GMIIGDLLK KNLHEVDLH 
      LTTCLLANV SICETTKKA KFVVTVYNP LSRSVNHYV 
      RLPVEGTSY KITGPDGEE EYDIFESIS SFDYITEET 
      KPSARELVF AARNIPPLG LKLYYVELV EGFDHLRPF 
      EHVTDEYYG SECDGFTID NASGMLSSI TIRELTLNI 
      TQEFFYYSG YSKFRPSGA YVFRPQDPL PKQFKSRKV 
      TVKAAKGKL ADEVLQIYN SEVSQIIRV YKNGEGYVE 
      FDWLVGNLQ THGLGKEVI TRFEVPGLK NKGVFFTDS 
      NGREQIKRE LNKRSDYEY DPRNEPVSS NYYPVTSKI 
      VIKDVENDL EVAVLNDRA QGGTSLKDG VIELMVHRK 
      LVRDDGYGV EEVLREREY HRGLYARGQ HYLVFGFVG 
      NDVKTEKSI SAYERDLAN QKLLAPWIL VADATKEKI 
      NSLEKTRKL INFQFNGLT KSLPENVKI LTLEPWRDN 
      TYILRLEHT LEEDEDWVL SQKVIVDLE NLFSTFSIC 
      EIGETTLGA NQWLGDYAE EEKLIWNDK LPTPWTKLN 
      PNDPLKIEL NPMQIRTFL IKVDTK
    CDS sequence
    • >TC006453 RA
      atgtttgca aaatttctc aaaatcgct ctattcata 
      aatttggtg ctatgtaaa ccacccaaa agactttct 
      gacacaatt cctgcaact tgtcacgct tgtcatccc 
      atagctcca gagaaaata aacattcat ttggtacca 
      cattcacat gatgatgta gggtgggtt aaaacaaca 
      gaacaatac tattatggc tccaaaaaa aaagtccag 
      tttgctgga gtccaatac attattagt tcaacaata 
      gaagcccta aaacgcaac cctaagcgc cgttttatt 
      caagtcgag tcagttttt ttttcgaaa tggtggcaa 
      aaccaggac gaaactctc agacaagaa gtgatcgaa 
      atggtcaag agaggccaa ctggaaatc gtcaacggt 
      gcctggacc atgaacgac gaagctgat acgaattat 
      cagtcgatc atcgaccag ttcacttgg ggttttagg 
      tttctggat gataatttg ggtaaatgt gccagacct 
      aaagtgggc tggcagatc gattcgttt ggacatagc 
      agagaacat gcctcgatt ttgacccaa atgggcttc 
      caaggcttg gtcattggt cgactggac cacagggat 
      aaagagaaa aggcagcag gagaagaat ttggacttt 
      gtgtggcaa gctagcgcc aatttgaac aattcggcg 
      attttcaca acaatgttt cccgaattt tacgtcagt 
      attgaatgc ttttgcttt gatatactc tgtgatgac 
      gaaatgatt aacgacgat gtgaaaagt cccgaattt 
      aatttgaac gaaaaagtc tatatgttt tcgcaagca 
      atggatcag tttaaggaa tactacaag accaataat 
      attctatta ccgatgggt ggcgatttt acgtaccaa 
      gcagccgag atcaatttt tccaatatc gataaactc 
      atcaaaggg tttcaggga cataagaaa tataacgtt 
      ttctactca actccttcg tgttacatt gcagctgtt 
      aatgaagaa gttaacaga aaagggatt aaactgcga 
      acgaaaact gacgatttt tttccatat tctttgaca 
      agagacgct ttctggact ggatattac acatcaaga 
      cccacttta aaacgattt gaaagggtc ggaaataac 
      attcttcaa gcagtcaag caattaacc acgttctcc 
      cgaatcaaa ggaaaggat aagtcatat tttattgat 
      aatctacgt ggtgctatg ggcattatg caacatcat 
      gatgctata actggtact gagagagag gcagttgct 
      aaagattac gtcaaaatg ctcaataaa gcaataaga 
      gaggcggaa acacccatt ggaatgatc attggtgat 
      cttttaaag aaaaattta catgaggtt gatttacat 
      ttaacgact tgtttgttg gccaatgtt agtatctgt 
      gagactacc aaaaaggcc aagtttgtg gtgactgtc 
      tacaaccct ttgtcacgt tctgtaaat cactatgtt 
      cgactaccc gttgaaggt acaagttat aaaattacg 
      ggacctgac ggtgaagaa gaatacgac atttttgaa 
      agcatctct tcttttgac tacatcacc gaagaaacg 
      aaaccaagc gccagagaa ctagttttc gcagccaga 
      aacatacca ccactcggc ctaaaattg tattatgtt 
      gaactagtg gaaggtttt gatcattta cgccctttc 
      gagcacgtc acagatgaa tattacgga tctgagtgt 
      gatggtttt acaattgat aacgcttcc ggaatgcta 
      tcttctata acaataaga gaactaaca ttgaacatt 
      actcaagaa tttttttat tattccggt tatagtaaa 
      ttccgccct tctggtgcg tacgttttc cggccacaa 
      gacccgcta ccaaaacaa ttcaaatca cgaaaagtc 
      acagttaaa gccgccaaa ggtaaactt gcggatgaa 
      gttttacaa atttacaat tcagaagtg agtcaaata 
      attcgcgtt tacaaaaac ggcgaaggt tacgttgaa 
      tttgactgg ttggtggga aatttacaa acacatgga 
      ttagggaaa gaagtgatc acgcgtttt gaggtgcca 
      ggtttgaag aacaaagga gtttttttc actgattca 
      aacggaagg gaacaaatc aaacgagag ttgaataaa 
      agaagtgat tatgagtac gatccaagg aatgaaccg 
      gtgtctagt aattactac ccagtcacg tcaaaaatc 
      gtgattaaa gacgtggag aatgatttg gaagttgcg 
      gttttgaac gatagagcc caaggtggt acgagttta 
      aaagacgga gttattgaa ttgatggtg catagaaaa 
      cttgtccgg gatgatggg tatggtgtt gaagaagtg 
      ctaagggag agggagtac catcgtggg ttgtatgca 
      agagggcaa cactacttg gtttttggt tttgttggc 
      aatgatgtt aaaacagaa aaatccata tctgcgtat 
      gaacgcgac ttagcaaat cagaaattg ttggcacct 
      tggatccta gttgctgat gctacgaaa gaaaaaata 
      aattcgttg gaaaaaaca cgaaaattg attaatttt 
      cagtttaac ggtcttaca aagtcactt ccggaaaat 
      gttaagatt ttgactttg gagccttgg cgggacaat 
      acttacatt ttacggttg gaacacact ttggaagaa 
      gatgaagat tgggttttg tcgcaaaaa gttatcgtt 
      gatttagaa aaccttttc tcgactttt tcaatttgt 
      gaaattggc gagacaacg ttgggagcg aatcagtgg 
      ttaggtgac tatgcagaa gaagagaaa ttgatatgg 
      aatgacaaa ttaccgacc ccttggaca aaattgaac 
      ccgaatgat cctctcaaa atcgaatta aatccaatg 
      caaataaga acgttttta ataaaagtt gacacaaaa 
      taa
    mRNA sequence
    • >TC006453 RA
      catcaaatg agagtaaag gacgcagga aacacatcc 
      acattcgaa gaatagaga ttttatcaa ctgttcaag 
      tggtgtttt accaataat gtttgcaaa atttctcaa 
      aatcgctct attcataaa tttggtgct atgtaaacc 
      acccaaaag actttctga cacaattcc tgcaacttg 
      tcacgcttg tcatcccat agctccaga gaaaataaa 
      cattcattt ggtaccaca ttcacatga tgatgtagg 
      gtgggttaa aacaacaga acaatacta ttatggctc 
      caaaaaaaa agtccagtt tgctggagt ccaatacat 
      tattagttc aacaataga agccctaaa acgcaaccc 
      taagcgccg ttttattca agtcgagtc agttttttt 
      ttcgaaatg gtggcaaaa ccaggacga aactctcag 
      acaagaagt gatcgaaat ggtcaagag aggccaact 
      ggaaatcgt caacggtgc ctggaccat gaacgacga 
      agctgatac gaattatca gtcgatcat cgaccagtt 
      cacttgggg ttttaggtt tctggatga taatttggg 
      taaatgtgc cagacctaa agtgggctg gcagatcga 
      ttcgtttgg acatagcag agaacatgc ctcgatttt 
      gacccaaat gggcttcca aggcttggt cattggtcg 
      actggacca cagggataa agagaaaag gcagcagga 
      gaagaattt ggactttgt gtggcaagc tagcgccaa 
      tttgaacaa ttcggcgat tttcacaac aatgtttcc 
      cgaatttta cgtcagtat tgaatgctt ttgctttga 
      tatactctg tgatgacga aatgattaa cgacgatgt 
      gaaaagtcc cgaatttaa tttgaacga aaaagtcta 
      tatgttttc gcaagcaat ggatcagtt taaggaata 
      ctacaagac caataatat tctattacc gatgggtgg 
      cgattttac gtaccaagc agccgagat caatttttc 
      caatatcga taaactcat caaagggtt tcagggaca 
      taagaaata taacgtttt ctactcaac tccttcgtg 
      ttacattgc agctgttaa tgaagaagt taacagaaa 
      agggattaa actgcgaac gaaaactga cgatttttt 
      tccatattc tttgacaag agacgcttt ctggactgg 
      atattacac atcaagacc cactttaaa acgatttga 
      aagggtcgg aaataacat tcttcaagc agtcaagca 
      attaaccac gttctcccg aatcaaagg aaaggataa 
      gtcatattt tattgataa tctacgtgg tgctatggg 
      cattatgca acatcatga tgctataac tggtactga 
      gagagaggc agttgctaa agattacgt caaaatgct 
      caataaagc aataagaga ggcggaaac acccattgg 
      aatgatcat tggtgatct tttaaagaa aaatttaca 
      tgaggttga tttacattt aacgacttg tttgttggc 
      caatgttag tatctgtga gactaccaa aaaggccaa 
      gtttgtggt gactgtcta caacccttt gtcacgttc 
      tgtaaatca ctatgttcg actacccgt tgaaggtac 
      aagttataa aattacggg acctgacgg tgaagaaga 
      atacgacat ttttgaaag catctcttc ttttgacta 
      catcaccga agaaacgaa accaagcgc cagagaact 
      agttttcgc agccagaaa cataccacc actcggcct 
      aaaattgta ttatgttga actagtgga aggttttga 
      tcatttacg ccctttcga gcacgtcac agatgaata 
      ttacggatc tgagtgtga tggttttac aattgataa 
      cgcttccgg aatgctatc ttctataac aataagaga 
      actaacatt gaacattac tcaagaatt tttttatta 
      ttccggtta tagtaaatt ccgcccttc tggtgcgta 
      cgttttccg gccacaaga cccgctacc aaaacaatt 
      caaatcacg aaaagtcac agttaaagc cgccaaagg 
      taaacttgc ggatgaagt tttacaaat ttacaattc 
      agaagtgag tcaaataat tcgcgttta caaaaacgg 
      cgaaggtta cgttgaatt tgactggtt ggtgggaaa 
      tttacaaac acatggatt agggaaaga agtgatcac 
      gcgttttga ggtgccagg tttgaagaa caaaggagt 
      ttttttcac tgattcaaa cggaaggga acaaatcaa 
      acgagagtt gaataaaag aagtgatta tgagtacga 
      tccaaggaa tgaaccggt gtctagtaa ttactaccc 
      agtcacgtc aaaaatcgt gattaaaga cgtggagaa 
      tgatttgga agttgcggt tttgaacga tagagccca 
      aggtggtac gagtttaaa agacggagt tattgaatt 
      gatggtgca tagaaaact tgtccggga tgatgggta 
      tggtgttga agaagtgct aagggagag ggagtacca 
      tcgtgggtt gtatgcaag agggcaaca ctacttggt 
      ttttggttt tgttggcaa tgatgttaa aacagaaaa 
      atccatatc tgcgtatga acgcgactt agcaaatca 
      gaaattgtt ggcaccttg gatcctagt tgctgatgc 
      tacgaaaga aaaaataaa ttcgttgga aaaaacacg 
      aaaattgat taattttca gtttaacgg tcttacaaa 
      gtcacttcc ggaaaatgt taagatttt gactttgga 
      gccttggcg ggacaatac ttacatttt acggttgga 
      acacacttt ggaagaaga tgaagattg ggttttgtc 
      gcaaaaagt tatcgttga tttagaaaa ccttttctc 
      gactttttc aatttgtga aattggcga gacaacgtt 
      gggagcgaa tcagtggtt aggtgacta tgcagaaga 
      agagaaatt gatatggaa tgacaaatt accgacccc 
      ttggacaaa attgaaccc gaatgatcc tctcaaaat 
      cgaattaaa tccaatgca aataagaac gtttttaat 
      aaaagttga cacaaaata atatttttt cgtgtaata 
      aatttatta ttcaagata ttttgtttt tattttatt 
      ccattttac atcgtaaaa taaaaacgt tcaaatgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY