| General information | |||
| TC006453 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9872071..9880143 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006453 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04145 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC006453 RA
MFAKFLKIA LFINLVLCK PPKRLSDTI PATCHACHP IAPEKINIH LVPHSHDDV GWVKTTEQY YYGSKKKVQ FAGVQYIIS STIEALKRN PKRRFIQVE SVFFSKWWQ NQDETLRQE VIEMVKRGQ LEIVNGAWT MNDEADTNY QSIIDQFTW GFRFLDDNL GKCARPKVG WQIDSFGHS REHASILTQ MGFQGLVIG RLDHRDKEK RQQEKNLDF VWQASANLN NSAIFTTMF PEFYVSIEC FCFDILCDD EMINDDVKS PEFNLNEKV YMFSQAMDQ FKEYYKTNN ILLPMGGDF TYQAAEINF SNIDKLIKG FQGHKKYNV FYSTPSCYI AAVNEEVNR KGIKLRTKT DDFFPYSLT RDAFWTGYY TSRPTLKRF ERVGNNILQ AVKQLTTFS RIKGKDKSY FIDNLRGAM GIMQHHDAI TGTEREAVA KDYVKMLNK AIREAETPI GMIIGDLLK KNLHEVDLH LTTCLLANV SICETTKKA KFVVTVYNP LSRSVNHYV RLPVEGTSY KITGPDGEE EYDIFESIS SFDYITEET KPSARELVF AARNIPPLG LKLYYVELV EGFDHLRPF EHVTDEYYG SECDGFTID NASGMLSSI TIRELTLNI TQEFFYYSG YSKFRPSGA YVFRPQDPL PKQFKSRKV TVKAAKGKL ADEVLQIYN SEVSQIIRV YKNGEGYVE FDWLVGNLQ THGLGKEVI TRFEVPGLK NKGVFFTDS NGREQIKRE LNKRSDYEY DPRNEPVSS NYYPVTSKI VIKDVENDL EVAVLNDRA QGGTSLKDG VIELMVHRK LVRDDGYGV EEVLREREY HRGLYARGQ HYLVFGFVG NDVKTEKSI SAYERDLAN QKLLAPWIL VADATKEKI NSLEKTRKL INFQFNGLT KSLPENVKI LTLEPWRDN TYILRLEHT LEEDEDWVL SQKVIVDLE NLFSTFSIC EIGETTLGA NQWLGDYAE EEKLIWNDK LPTPWTKLN PNDPLKIEL NPMQIRTFL IKVDTK
- >TC006453 RA
atgtttgca aaatttctc aaaatcgct ctattcata aatttggtg ctatgtaaa ccacccaaa agactttct gacacaatt cctgcaact tgtcacgct tgtcatccc atagctcca gagaaaata aacattcat ttggtacca cattcacat gatgatgta gggtgggtt aaaacaaca gaacaatac tattatggc tccaaaaaa aaagtccag tttgctgga gtccaatac attattagt tcaacaata gaagcccta aaacgcaac cctaagcgc cgttttatt caagtcgag tcagttttt ttttcgaaa tggtggcaa aaccaggac gaaactctc agacaagaa gtgatcgaa atggtcaag agaggccaa ctggaaatc gtcaacggt gcctggacc atgaacgac gaagctgat acgaattat cagtcgatc atcgaccag ttcacttgg ggttttagg tttctggat gataatttg ggtaaatgt gccagacct aaagtgggc tggcagatc gattcgttt ggacatagc agagaacat gcctcgatt ttgacccaa atgggcttc caaggcttg gtcattggt cgactggac cacagggat aaagagaaa aggcagcag gagaagaat ttggacttt gtgtggcaa gctagcgcc aatttgaac aattcggcg attttcaca acaatgttt cccgaattt tacgtcagt attgaatgc ttttgcttt gatatactc tgtgatgac gaaatgatt aacgacgat gtgaaaagt cccgaattt aatttgaac gaaaaagtc tatatgttt tcgcaagca atggatcag tttaaggaa tactacaag accaataat attctatta ccgatgggt ggcgatttt acgtaccaa gcagccgag atcaatttt tccaatatc gataaactc atcaaaggg tttcaggga cataagaaa tataacgtt ttctactca actccttcg tgttacatt gcagctgtt aatgaagaa gttaacaga aaagggatt aaactgcga acgaaaact gacgatttt tttccatat tctttgaca agagacgct ttctggact ggatattac acatcaaga cccacttta aaacgattt gaaagggtc ggaaataac attcttcaa gcagtcaag caattaacc acgttctcc cgaatcaaa ggaaaggat aagtcatat tttattgat aatctacgt ggtgctatg ggcattatg caacatcat gatgctata actggtact gagagagag gcagttgct aaagattac gtcaaaatg ctcaataaa gcaataaga gaggcggaa acacccatt ggaatgatc attggtgat cttttaaag aaaaattta catgaggtt gatttacat ttaacgact tgtttgttg gccaatgtt agtatctgt gagactacc aaaaaggcc aagtttgtg gtgactgtc tacaaccct ttgtcacgt tctgtaaat cactatgtt cgactaccc gttgaaggt acaagttat aaaattacg ggacctgac ggtgaagaa gaatacgac atttttgaa agcatctct tcttttgac tacatcacc gaagaaacg aaaccaagc gccagagaa ctagttttc gcagccaga aacatacca ccactcggc ctaaaattg tattatgtt gaactagtg gaaggtttt gatcattta cgccctttc gagcacgtc acagatgaa tattacgga tctgagtgt gatggtttt acaattgat aacgcttcc ggaatgcta tcttctata acaataaga gaactaaca ttgaacatt actcaagaa tttttttat tattccggt tatagtaaa ttccgccct tctggtgcg tacgttttc cggccacaa gacccgcta ccaaaacaa ttcaaatca cgaaaagtc acagttaaa gccgccaaa ggtaaactt gcggatgaa gttttacaa atttacaat tcagaagtg agtcaaata attcgcgtt tacaaaaac ggcgaaggt tacgttgaa tttgactgg ttggtggga aatttacaa acacatgga ttagggaaa gaagtgatc acgcgtttt gaggtgcca ggtttgaag aacaaagga gtttttttc actgattca aacggaagg gaacaaatc aaacgagag ttgaataaa agaagtgat tatgagtac gatccaagg aatgaaccg gtgtctagt aattactac ccagtcacg tcaaaaatc gtgattaaa gacgtggag aatgatttg gaagttgcg gttttgaac gatagagcc caaggtggt acgagttta aaagacgga gttattgaa ttgatggtg catagaaaa cttgtccgg gatgatggg tatggtgtt gaagaagtg ctaagggag agggagtac catcgtggg ttgtatgca agagggcaa cactacttg gtttttggt tttgttggc aatgatgtt aaaacagaa aaatccata tctgcgtat gaacgcgac ttagcaaat cagaaattg ttggcacct tggatccta gttgctgat gctacgaaa gaaaaaata aattcgttg gaaaaaaca cgaaaattg attaatttt cagtttaac ggtcttaca aagtcactt ccggaaaat gttaagatt ttgactttg gagccttgg cgggacaat acttacatt ttacggttg gaacacact ttggaagaa gatgaagat tgggttttg tcgcaaaaa gttatcgtt gatttagaa aaccttttc tcgactttt tcaatttgt gaaattggc gagacaacg ttgggagcg aatcagtgg ttaggtgac tatgcagaa gaagagaaa ttgatatgg aatgacaaa ttaccgacc ccttggaca aaattgaac ccgaatgat cctctcaaa atcgaatta aatccaatg caaataaga acgttttta ataaaagtt gacacaaaa taa
- >TC006453 RA
catcaaatg agagtaaag gacgcagga aacacatcc acattcgaa gaatagaga ttttatcaa ctgttcaag tggtgtttt accaataat gtttgcaaa atttctcaa aatcgctct attcataaa tttggtgct atgtaaacc acccaaaag actttctga cacaattcc tgcaacttg tcacgcttg tcatcccat agctccaga gaaaataaa cattcattt ggtaccaca ttcacatga tgatgtagg gtgggttaa aacaacaga acaatacta ttatggctc caaaaaaaa agtccagtt tgctggagt ccaatacat tattagttc aacaataga agccctaaa acgcaaccc taagcgccg ttttattca agtcgagtc agttttttt ttcgaaatg gtggcaaaa ccaggacga aactctcag acaagaagt gatcgaaat ggtcaagag aggccaact ggaaatcgt caacggtgc ctggaccat gaacgacga agctgatac gaattatca gtcgatcat cgaccagtt cacttgggg ttttaggtt tctggatga taatttggg taaatgtgc cagacctaa agtgggctg gcagatcga ttcgtttgg acatagcag agaacatgc ctcgatttt gacccaaat gggcttcca aggcttggt cattggtcg actggacca cagggataa agagaaaag gcagcagga gaagaattt ggactttgt gtggcaagc tagcgccaa tttgaacaa ttcggcgat tttcacaac aatgtttcc cgaatttta cgtcagtat tgaatgctt ttgctttga tatactctg tgatgacga aatgattaa cgacgatgt gaaaagtcc cgaatttaa tttgaacga aaaagtcta tatgttttc gcaagcaat ggatcagtt taaggaata ctacaagac caataatat tctattacc gatgggtgg cgattttac gtaccaagc agccgagat caatttttc caatatcga taaactcat caaagggtt tcagggaca taagaaata taacgtttt ctactcaac tccttcgtg ttacattgc agctgttaa tgaagaagt taacagaaa agggattaa actgcgaac gaaaactga cgatttttt tccatattc tttgacaag agacgcttt ctggactgg atattacac atcaagacc cactttaaa acgatttga aagggtcgg aaataacat tcttcaagc agtcaagca attaaccac gttctcccg aatcaaagg aaaggataa gtcatattt tattgataa tctacgtgg tgctatggg cattatgca acatcatga tgctataac tggtactga gagagaggc agttgctaa agattacgt caaaatgct caataaagc aataagaga ggcggaaac acccattgg aatgatcat tggtgatct tttaaagaa aaatttaca tgaggttga tttacattt aacgacttg tttgttggc caatgttag tatctgtga gactaccaa aaaggccaa gtttgtggt gactgtcta caacccttt gtcacgttc tgtaaatca ctatgttcg actacccgt tgaaggtac aagttataa aattacggg acctgacgg tgaagaaga atacgacat ttttgaaag catctcttc ttttgacta catcaccga agaaacgaa accaagcgc cagagaact agttttcgc agccagaaa cataccacc actcggcct aaaattgta ttatgttga actagtgga aggttttga tcatttacg ccctttcga gcacgtcac agatgaata ttacggatc tgagtgtga tggttttac aattgataa cgcttccgg aatgctatc ttctataac aataagaga actaacatt gaacattac tcaagaatt tttttatta ttccggtta tagtaaatt ccgcccttc tggtgcgta cgttttccg gccacaaga cccgctacc aaaacaatt caaatcacg aaaagtcac agttaaagc cgccaaagg taaacttgc ggatgaagt tttacaaat ttacaattc agaagtgag tcaaataat tcgcgttta caaaaacgg cgaaggtta cgttgaatt tgactggtt ggtgggaaa tttacaaac acatggatt agggaaaga agtgatcac gcgttttga ggtgccagg tttgaagaa caaaggagt ttttttcac tgattcaaa cggaaggga acaaatcaa acgagagtt gaataaaag aagtgatta tgagtacga tccaaggaa tgaaccggt gtctagtaa ttactaccc agtcacgtc aaaaatcgt gattaaaga cgtggagaa tgatttgga agttgcggt tttgaacga tagagccca aggtggtac gagtttaaa agacggagt tattgaatt gatggtgca tagaaaact tgtccggga tgatgggta tggtgttga agaagtgct aagggagag ggagtacca tcgtgggtt gtatgcaag agggcaaca ctacttggt ttttggttt tgttggcaa tgatgttaa aacagaaaa atccatatc tgcgtatga acgcgactt agcaaatca gaaattgtt ggcaccttg gatcctagt tgctgatgc tacgaaaga aaaaataaa ttcgttgga aaaaacacg aaaattgat taattttca gtttaacgg tcttacaaa gtcacttcc ggaaaatgt taagatttt gactttgga gccttggcg ggacaatac ttacatttt acggttgga acacacttt ggaagaaga tgaagattg ggttttgtc gcaaaaagt tatcgttga tttagaaaa ccttttctc gactttttc aatttgtga aattggcga gacaacgtt gggagcgaa tcagtggtt aggtgacta tgcagaaga agagaaatt gatatggaa tgacaaatt accgacccc ttggacaaa attgaaccc gaatgatcc tctcaaaat cgaattaaa tccaatgca aataagaac gtttttaat aaaagttga cacaaaata atatttttt cgtgtaata aatttatta ttcaagata ttttgtttt tattttatt ccattttac atcgtaaaa taaaaacgt tcaaatgaa
