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Gene information
General information
TC006450 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9804046..9808340
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006450
    iBeetle screen iB_04144

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006450 RA
      MFTTKAFIK KNKVGNYLK HVREHYIRD DISCGLQEC 
      ETCLPVESF LSPDHENKC SLFTSNHYL ILDTNVVLD 
      QMDILEESV IGNVIILHT VLNEVKHRS LPIYKRLCA 
      VIENPSRHF YVFINDHHQ GTYLQQNEG ELINDYNDR 
      CIRAACSWY INHAPKQKF VFLTDDSAN RIKAQKEDI 
      PSYTVSEYV QNLNTSGLT DKLSRKDFH GNDFTKDVF 
      PNHLSNVEL LTGIREGKL LQGTFRASR DNFLEGFVN 
      VDSYEEPVI VQGRAGLNR SVDGDTVAV ELLKEEEWV 
      CPSEIILED EGVGDESVD DVVTKENEL QKATKKKSG 
      AKPTGRVVG IIRRKWRQY CGILQGGID GVYQMFVPA 
      DKSIPKIKI ETRQAEFLR TQKLIVTID SWPKHSRYP 
      HGHFVRALG NIGDQTTEN EVILLEHDV PHSQFSEEV 
      LNCLPKMPW IITDDDVKK RVDLRHLDI CSVDPPGCT 
      DIDDALHCR PLADGKLEV GVHIADVSH FIRPGTPLD 
      REAASRATT VYLVNNRID MVPALLSSN LCSLRGGEE 
      RFAFSCIWE MDQNANILS TRFHKSIIK SRKAMTYEE 
      AQLIIDDKT KQDALAEAL RSLNKLAKI MKQRRLVNG 
      ALVLASPEV KIQMDSETM EPLDVEVKK LFETNSMIE 
      EFMLQANIS VAEKILEEF PDCAMLRRH PVPPATNFD 
      PLVKAGRHL GFEIRVDSG KNLAKSLDE AVNTKNPYL 
      NTMFRILAT RCMLQAVYF VSGTYQKEE YFHYGLAVP 
      LYTHFTSPI RRYADIIVH RLLAVICGA DSTYPDLLD 
      KNKTTELCQ NLNYRNRMA QYAGRASIA FNTHGNYKM 
      RKVTCCMSV KTPCKFLYH DMAWNVPYL WPKKAKRPI 
      FLNTMKRTR HKKPEVWLF TLLTPL
    CDS sequence
    • >TC006450 RA
      atgtttacg actaaagct tttattaaa aagaacaaa 
      gttgggaat tatctgaag cacgttcga gaacattat 
      attcgtgat gacatttct tgtggtctt caagagtgt 
      gaaacttgt ttgccagtg gagtcattc ctgtcccca 
      gaccacgaa aacaaatgt tctttattt acttctaat 
      cattatcta atactagac acaaatgtt gttcttgat 
      cagatggat attttggaa gagtccgtc attggtaat 
      gtgataatc ctccacaca gttttaaac gaggtgaag 
      caccgaagt ctaccaatt tacaaacgc ttgtgtgct 
      gtaattgaa aatccgagt cgtcatttt tacgttttt 
      ataaacgac caccatcaa ggcacttat ttgcaacaa 
      aacgaaggt gaattaatt aatgattac aatgacagg 
      tgtattaga gctgcgtgt tcctggtac ataaaccat 
      gcccctaaa caaaaattc gttttcctc actgacgac 
      tcagctaat cggattaaa gcccaaaaa gaggatata 
      ccttcttac accgtctcg gaatatgtg caaaatctc 
      aacacttcg ggtcttacc gataaatta tcgaggaaa 
      gattttcac ggaaatgat tttacgaaa gacgttttt 
      cccaaccat ttgagtaat gttgagctt ttgactggc 
      ataagagag ggaaagctg ctccagggg actttccga 
      gcctctcga gataatttc ttggaaggt tttgtcaat 
      gtggattcg tacgaagag ccggtgata gttcagggt 
      cgggctggc cttaaccgc tcagtggat ggtgacact 
      gtagctgtt gagcttttg aaagaggag gaatgggtg 
      tgtccgagt gaaataatc ctagaggat gaaggggtt 
      ggtgatgag agtgttgac gatgttgtg acaaaagaa 
      aacgagtta caaaaagcg acaaagaag aaaagtgga 
      gctaaacct acaggacgt gttgttgga attattcgt 
      agaaagtgg agacaatat tgtggcatt ttacaaggg 
      ggcattgat ggtgtttat cagatgttt gttccagct 
      gataaaagt atacccaaa attaaaata gaaacgaga 
      caagcggag tttctaagg actcagaaa ttgatcgtt 
      acgattgat tcctggccc aaacattca cgctatcct 
      catggtcat tttgtgcga gctttgggc aacattggg 
      gaccaaacc accgaaaac gaggtcatt ttgctggaa 
      catgacgtc cctcacagc caattctct gaggaagtt 
      ctcaattgt ttgcccaaa atgccttgg atcataacc 
      gatgatgac gtcaaaaaa cgagtcgat ctcagacac 
      cttgacatt tgctcagtg gatccccca ggttgtacc 
      gacattgac gacgccctt cattgtcgt ccactagct 
      gacggcaaa cttgaagtt ggtgttcat attgccgat 
      gtttcgcat tttataaga cctggaacg cctctagat 
      cgcgaagct gcttctaga gccaccaca gtgtatttg 
      gttaataat cgaatagat atggtgcca gcattgctc 
      agttcaaat ctttgttcg ttacgagga ggagaggaa 
      agattcgcg ttttcttgc atttgggaa atggaccaa 
      aatgccaat attttaagt acgcgattt cataaaagt 
      ataattaag tcgaggaaa gcaatgact tatgaagag 
      gcgcaactg attattgac gacaagacc aagcaggac 
      gctctggcc gaggccttg aggagcttg aacaagttg 
      gccaaaatt atgaagcag cgcagactg gttaatgga 
      gctttagtt cttgcatcg cctgaggta aaaatccag 
      atggactcc gagacgatg gaacccttg gacgtggaa 
      gtgaaaaaa ctgtttgaa acaaattcc atgattgag 
      gaatttatg ctgcaagca aatataagt gtcgctgaa 
      aaaatcctt gaggagttt cccgattgt gcgatgtta 
      cgtcgtcat cccgtccct ccagctact aatttcgat 
      cccctggtt aaagcaggc cgtcacctt ggctttgaa 
      attagagtg gattcaggc aaaaacttg gcaaaatca 
      ctcgatgaa gcagtaaac acaaaaaat ccctatttg 
      aacacaatg ttccgaatt ttagccaca cgttgtatg 
      ctacaagct gtttacttt gtcagtggg acgtaccaa 
      aaggaggag tacttccat tatggtcta gctgtgcct 
      ttgtacaca catttcacg tcaccaatt cgtagatac 
      gccgatatc atagtccac aggctactt gcagttatc 
      tgtggtgcc gactccact tatccggac cttttggat 
      aagaataaa acgaccgag ttgtgccaa aatttgaac 
      tacaggaac cgaatggct caatatgct ggaagagcc 
      tcaattgcg ttcaacaca catggaaac tacaagatg 
      aggaaggtt acgtgttgt atgtccgta aaaacgccc 
      tgcaagttc ttataccac gatatggcc tggaatgta 
      ccctatttg tggccaaaa aaggcgaaa cgtccaata 
      tttttgaat acaatgaag aggaccaga cacaaaaag 
      ccggaagtg tggttattc acgcttttg accccgttg 
      tag
    mRNA sequence
    • >TC006450 RA
      taaccacaa aaaatattg taatataac ctccaaata 
      acttgttgc ggcatgttt ttgtaaaaa aacatttga 
      aataattaa acaatgttt acgactaaa gcttttatt 
      aaaaagaac aaagttggg aattatctg aagcacgtt 
      cgagaacat tatattcgt gatgacatt tcttgtggt 
      cttcaagag tgtgaaact tgtttgcca gtggagtca 
      ttcctgtcc ccagaccac gaaaacaaa tgttcttta 
      tttacttct aatcattat ctaatacta gacacaaat 
      gttgttctt gatcagatg gatattttg gaagagtcc 
      gtcattggt aatgtgata atcctccac acagtttta 
      aacgaggtg aagcaccga agtctacca atttacaaa 
      cgcttgtgt gctgtaatt gaaaatccg agtcgtcat 
      ttttacgtt tttataaac gaccaccat caaggcact 
      tatttgcaa caaaacgaa ggtgaatta attaatgat 
      tacaatgac aggtgtatt agagctgcg tgttcctgg 
      tacataaac catgcccct aaacaaaaa ttcgttttc 
      ctcactgac gactcagct aatcggatt aaagcccaa 
      aaagaggat ataccttct tacaccgtc tcggaatat 
      gtgcaaaat ctcaacact tcgggtctt accgataaa 
      ttatcgagg aaagatttt cacggaaat gattttacg 
      aaagacgtt tttcccaac catttgagt aatgttgag 
      cttttgact ggcataaga gagggaaag ctgctccag 
      gggactttc cgagcctct cgagataat ttcttggaa 
      ggttttgtc aatgtggat tcgtacgaa gagccggtg 
      atagttcag ggtcgggct ggccttaac cgctcagtg 
      gatggtgac actgtagct gttgagctt ttgaaagag 
      gaggaatgg gtgtgtccg agtgaaata atcctagag 
      gatgaaggg gttggtgat gagagtgtt gacgatgtt 
      gtgacaaaa gaaaacgag ttacaaaaa gcgacaaag 
      aagaaaagt ggagctaaa cctacagga cgtgttgtt 
      ggaattatt cgtagaaag tggagacaa tattgtggc 
      attttacaa gggggcatt gatggtgtt tatcagatg 
      tttgttcca gctgataaa agtataccc aaaattaaa 
      atagaaacg agacaagcg gagtttcta aggactcag 
      aaattgatc gttacgatt gattcctgg cccaaacat 
      tcacgctat cctcatggt cattttgtg cgagctttg 
      ggcaacatt ggggaccaa accaccgaa aacgaggtc 
      attttgctg gaacatgac gtccctcac agccaattc 
      tctgaggaa gttctcaat tgtttgccc aaaatgcct 
      tggatcata accgatgat gacgtcaaa aaacgagtc 
      gatctcaga caccttgac atttgctca gtggatccc 
      ccaggttgt accgacatt gacgacgcc cttcattgt 
      cgtccacta gctgacggc aaacttgaa gttggtgtt 
      catattgcc gatgtttcg cattttata agacctgga 
      acgcctcta gatcgcgaa gctgcttct agagccacc 
      acagtgtat ttggttaat aatcgaata gatatggtg 
      ccagcattg ctcagttca aatctttgt tcgttacga 
      ggaggagag gaaagattc gcgttttct tgcatttgg 
      gaaatggac caaaatgcc aatatttta agtacgcga 
      tttcataaa agtataatt aagtcgagg aaagcaatg 
      acttatgaa gaggcgcaa ctgattatt gacgacaag 
      accaagcag gacgctctg gccgaggcc ttgaggagc 
      ttgaacaag ttggccaaa attatgaag cagcgcaga 
      ctggttaat ggagcttta gttcttgca tcgcctgag 
      gtaaaaatc cagatggac tccgagacg atggaaccc 
      ttggacgtg gaagtgaaa aaactgttt gaaacaaat 
      tccatgatt gaggaattt atgctgcaa gcaaatata 
      agtgtcgct gaaaaaatc cttgaggag tttcccgat 
      tgtgcgatg ttacgtcgt catcccgtc cctccagct 
      actaatttc gatcccctg gttaaagca ggccgtcac 
      cttggcttt gaaattaga gtggattca ggcaaaaac 
      ttggcaaaa tcactcgat gaagcagta aacacaaaa 
      aatccctat ttgaacaca atgttccga attttagcc 
      acacgttgt atgctacaa gctgtttac tttgtcagt 
      gggacgtac caaaaggag gagtacttc cattatggt 
      ctagctgtg cctttgtac acacatttc acgtcacca 
      attcgtaga tacgccgat atcatagtc cacaggcta 
      cttgcagtt atctgtggt gccgactcc acttatccg 
      gaccttttg gataagaat aaaacgacc gagttgtgc 
      caaaatttg aactacagg aaccgaatg gctcaatat 
      gctggaaga gcctcaatt gcgttcaac acacatgga 
      aactacaag atgaggaag gttacgtgt tgtatgtcc 
      gtaaaaacg ccctgcaag ttcttatac cacgatatg 
      gcctggaat gtaccctat ttgtggcca aaaaaggcg 
      aaacgtcca atatttttg aatacaatg aagaggacc 
      agacacaaa aagccggaa gtgtggtta ttcacgctt 
      ttgaccccg ttgtagtcc gactatcgc ttgattcta 
      ataacattc aacacgaaa aatttgttt tacaactcg 
      ttaaacctt acgttgagg gtttcagtg ttgcgccac 
      tggaaagta acgatgaaa atatgaagc gcaagcaac 
      cggaaccag agacaggta aaaagaaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY