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Gene information
General information
TC006432 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9193575..9231590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006432
    iBeetle screen iB_04141

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006432 RA
      MYVTCLGLI NLVVLTWSV DAQHYQGLN DLDILPEGK 
      PPSFVTPGQ TYRIAAGST VVLPCRITP AGAYVLAWK 
      RGIAVLTAG PVKVTPDPR VRLLPSPVS TSSLTGIPE 
      LSGEGYSLE LKDVRPQDA GDYVCQIGT MEPREITHT 
      LEILVPPRI HYVSHTGPL EVLQGATVK MECRASGNP 
      VPTVAWTRK NNVLPSGER SVSGLSLVI QRADRHSAG 
      QYQCSADNG VGQPDTRHI TLNVLYGPE VETERATVH 
      TGIGLEAQL VCIVHAEPA PQVLWFKDT AQLGTTEQH 
      AAHARGNRY TLVIRNVTS ADFGNYSCV ASNSHGKGR 
      GQLTLTGTA GLAVFDSPP LSSEKDSYN VSWSVNSHA 
      AITEYRLFY RQQPHHHHG HHVEMYNNT KFTYRNDWN 
      SVVLPGAGV VNVQFPLPP PYPGVTRQR MFYVIKSLQ 
      PSTTYEARV QAKNQHGWN KLSPIFHFT TRSIEMENM 
      VEPSAQPAV YGVSQQGMA PFSSGVREQ FTSASVILV 
      LVLKFF
    CDS sequence
    • >TC006432 RA
      atgtacgta acgtgtctc gggctaatt aacctggtg 
      gtgctcacc tggagcgta gacgcccag cactaccaa 
      ggcctcaat gacctggac atcctcccc gagggcaag 
      cccccaagt tttgtaact ccgggccag acttaccgc 
      atagccgcc ggaagcacg gtggtttta ccttgcagg 
      ataactccg gcaggcgct tacgtgctg gcgtggaaa 
      cgaggaatt gcagttttg acagccgga ccagtgaaa 
      gtcacccct gatccgagg gtgagactg ctgccgtcc 
      ccggtgagc acttcgagc ttaaccgga ataccggaa 
      ctatccgga gaaggatat agtttggaa ttgaaggat 
      gttagaccg caagatgcc ggagattat gtgtgccaa 
      attggaaca atggagccg agagaaatt acacatact 
      ttggagata ttggtgcct ccccgaatc cattacgtt 
      tcccacacc ggtccattg gaagtcctc cagggtgct 
      acagtgaaa atggagtgt cgagcgtcg ggcaatccc 
      gtgccgacc gtcgcctgg accagaaag aacaacgtt 
      ttgccaagc ggcgagcgt tcggtttcc ggtttatct 
      ctcgtaatt cagcgagcg gaccgccat tccgcaggc 
      caatatcaa tgctcggct gataacggc gtcggccaa 
      cctgacaca cgtcatatc acccttaac gttttatac 
      ggaccagaa gtggaaact gaaagagcg actgtacac 
      acgggaatt ggacttgaa gcacagttg gtgtgtata 
      gttcatgca gaaccagct ccacaggtg ctttggttt 
      aaggatacg gcccaactg ggcaccaca gaacaacat 
      gcagcgcac gcaagaggc aacagatac accctcgtt 
      attcgcaac gtcacttcg gcggacttt ggaaactac 
      agctgcgtt gcgagcaat tcgcacggg aaaggccgc 
      ggacagctc actttaacc gggactgca ggactggct 
      gtcttcgac tcgccgcct cttagctcc gagaaggac 
      tcgtacaac gtgtcctgg tcggtcaac agccacgcc 
      gccatcacc gaataccgc ctgttttac agacaacag 
      ccccaccac caccacgga caccacgtc gaaatgtac 
      aacaacacc aagtttacc tatcgcaac gactggaac 
      agcgttgtg cttcctggg gccggggtt gtcaacgtc 
      caattcccc ctaccgccg ccctatcca ggggtgacc 
      cgccaacgg atgttttac gttatcaag tctctgcaa 
      ccctccaca acctacgag gcaagagtt caagccaag 
      aaccagcac ggttggaac aagttatcg cccattttc 
      cacttcacg actagatca atcgaaatg gaaaatatg 
      gtagaacca tccgcccaa ccggcagtt tacggggtg 
      agtcagcag ggaatggcc cccttcagc agtggcgtt 
      cgcgaacag ttcacctcc gccagtgtg attctcgtg 
      ctagtgctg aagttcttt taa
    mRNA sequence
    • >TC006432 RA
      accctccag tatttcccc ggcgtttgt ctgaaatct 
      atcggagct ttggaggtt tcggcgcgc tttcggcaa 
      ctttcaaac tcgtgaact gtgctctcg agtgagaat 
      tgtgagtga aactgtgac aatgtacgt aacgtgtct 
      cgggctaat taacctggt ggtgctcac ctggagcgt 
      agacgccca gcactacca aggcctcaa tgacctgga 
      catcctccc cgagggcaa gcccccaag ttttgtaac 
      tccgggcca gacttaccg catagccgc cggaagcac 
      ggtggtttt accttgcag gataactcc ggcaggcgc 
      ttacgtgct ggcgtggaa acgaggaat tgcagtttt 
      gacagccgg accagtgaa agtcacccc tgatccgag 
      ggtgagact gctgccgtc cccggtgag cacttcgag 
      cttaaccgg aataccgga actatccgg agaaggata 
      tagtttgga attgaagga tgttagacc gcaagatgc 
      cggagatta tgtgtgcca aattggaac aatggagcc 
      gagagaaat tacacatac tttggagat attggtgcc 
      tccccgaat ccattacgt ttcccacac cggtccatt 
      ggaagtcct ccagggtgc tacagtgaa aatggagtg 
      tcgagcgtc gggcaatcc cgtgccgac cgtcgcctg 
      gaccagaaa gaacaacgt tttgccaag cggcgagcg 
      ttcggtttc cggtttatc tctcgtaat tcagcgagc 
      ggaccgcca ttccgcagg ccaatatca atgctcggc 
      tgataacgg cgtcggcca acctgacac acgtcatat 
      cacccttaa cgttttata cggaccaga agtggaaac 
      tgaaagagc gactgtaca cacgggaat tggacttga 
      agcacagtt ggtgtgtat agttcatgc agaaccagc 
      tccacaggt gctttggtt taaggatac ggcccaact 
      gggcaccac agaacaaca tgcagcgca cgcaagagg 
      caacagata caccctcgt tattcgcaa cgtcacttc 
      ggcggactt tggaaacta cagctgcgt tgcgagcaa 
      ttcgcacgg gaaaggccg cggacagct cactttaac 
      cgggactgc aggactggc tgtcttcga ctcgccgcc 
      tcttagctc cgagaagga ctcgtacaa cgtgtcctg 
      gtcggtcaa cagccacgc cgccatcac cgaataccg 
      cctgtttta cagacaaca gccccacca ccaccacgg 
      acaccacgt cgaaatgta caacaacac caagtttac 
      ctatcgcaa cgactggaa cagcgttgt gcttcctgg 
      ggccggggt tgtcaacgt ccaattccc cctaccgcc 
      gccctatcc aggggtgac ccgccaacg gatgtttta 
      cgttatcaa gtctctgca accctccac aacctacga 
      ggcaagagt tcaagccaa gaaccagca cggttggaa 
      caagttatc gcccatttt ccacttcac gactagatc 
      aatcgaaat ggaaaatat ggtagaacc atccgccca 
      accggcagt ttacggggt gagtcagca gggaatggc 
      ccccttcag cagtggcgt tcgcgaaca gttcacctc 
      cgccagtgt gattctcgt gctagtgct gaagttctt 
      ttaaaccga agtgaagtg aattaaacc aaagtgttg 
      gtaccacgt acaatattg tgataactg tggatttaa 
      ggtgagaat tgacaacgc ctcgatttt ttcatgtga 
      caagtgacg attttttcc gatgttgct tattaagtg 
      tatagatat tacatttgt tcatctaag ataattcac 
      gtttagata gaggcgcgg aacatttta ttatttcta 
      ggctttgga agacacttt gtttcaatg agagggttc 
      agtcgttca ctacgtgta ctagttatc ataggacta 
      actttatat cgttatcac tctccatgg tgtcttttc 
      cctattgtt gtttacgag tgttttgcg ttcgaacca 
      attgtcaat gtaaattcg gttacatca tttatcact 
      gcttttttg cttgcacat attcgtaaa tgtcattgt 
      atataaaga ctgaactat ttttataag agtgcaata 
      agatgtatt agttctata actgtttca atgataacg 
      atgtcgaat aacatgtaa ataaagctt aaatagtac 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY