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Gene information
General information
TC006428 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9058964..9066246
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006428
    iBeetle screen iB_04139

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006428 RA
      MASVSTSFF IPDRSTPLP RPPEDEDSF PVICDRELH 
      SLHRNVPAL RRRDVDTAT IKQHYYPEG GWGWLVCGA 
      AFLAHLLTS GFQLAFGLL YLYTMKFLI RKNQDKDFY 
      VMATAWLGA LCLCISFLT APLIVAVCR RKSTRLAAA 
      FGGLVTALA CLFTSFAVQ FHQALLSYG LVLGLGAGV 
      VRETSGLML GHYFKRRRE FVEMVAQAG AGVGIALFS 
      VLYKEAVGK LGWRLGLQA ITGLLSLAF FLPVVYRSA 
      NLYHPQRRA ITHLKNQRK KMKEKKTHA KAKKPPMFD 
      FTPLKSRGL RVLMVACAF AALGIYAPV FYLVYQGYK 
      EGLEDSALV LLQTFMGFA SALGCVGFG LVTVRPSSQ 
      CLISRQYLC QAAMVGIGV SLLALSAVQ GYHGYVLFV 
      WLYGTCLGG YTYSLKMFA LERVRGRHF TKAWSFIQG 
      AKSLPVLLG IPITGYINQ TYPKAGYYF SFVTTIVGA 
      SLMFLVGTR KDHINSNTN NCNLNGCNI ANLNECACN 
      LALNYNNIC TPDLQPYNF CRQSANHYE RLFNYGHMD 
      RSDFHRHSL RHHYLPKSL SYAANIDRS PYHQYPVHY 
      HDYVRLTNP KSRNMLRPS KSVPEGLAR WDYCGSYRR 
      PIIRNVQVI EQITTSV
    CDS sequence
    • >TC006428 RA
      atggctagt gtttccacg tcgtttttt attccagac 
      cgcagcacg cctctccct cggccgccg gaagacgaa 
      gattcgttt cctgttatt tgcgaccga gaattgcat 
      tcattgcat cgaaacgtg cccgcactt cgacggagg 
      gacgtcgat acggccact attaagcag cactactac 
      cctgaagga ggatggggt tggttggtg tgcggagct 
      gccttctta gcccacctt ctcacctcc ggcttccaa 
      ttggcgttc ggactgctg tatttgtat accatgaag 
      ttcctcatt aggaaaaat caagacaaa gatttctac 
      gtgatggca actgcttgg ttgggagct ttgtgcctg 
      tgtatctct tttctgacc gctccatta attgtggcc 
      gtttgccga agaaagtcc acgagattg gcggccgcg 
      ttcgggggc cttgtgacg gctctggcg tgccttttt 
      acgtctttt gccgtacag ttccaccag gcactgctc 
      agctacggg ctggtgctg ggcctgggc gccggagtc 
      gtccgggag acttccggg ctgatgctg ggccactat 
      ttcaagcgg aggagagag ttcgtggaa atggtggcg 
      caagccgga gccggggtc gggatcgcc ttgttttcg 
      gtgctttat aaagaagca gtggggaaa ctgggatgg 
      agattaggc ttgcaagcc atcaccggt ttactctcc 
      ctcgcattt tttctccct gttgtctac cgatcggcc 
      aatttgtac cacccgcag cgacgtgcc attacacat 
      ttaaaaaat caacgcaaa aaaatgaaa gaaaagaag 
      acgcacgcc aaagctaag aaaccgcca atgttcgat 
      ttcacgccg ctgaaaagt cgcggattg cgggtattg 
      atggtggct tgcgctttc gctgcgtta ggaatttac 
      gcccccgtt ttctacctt gtgtaccag gggtataag 
      gagggatta gaagacagt gctttagtt ctcttacaa 
      actttcatg gggtttgcg tcagcttta ggctgcgtc 
      ggatttggg ctggtgact gtgaggccc agctcgcag 
      tgcttaatt tccaggcaa tatttgtgt caagcggcc 
      atggtaggg atcggagtg tctcttctg gcgctaagt 
      gccgttcag ggataccac gggtacgtt ttgtttgtc 
      tggctgtat ggcacttgt ctcgggggc tacacctac 
      tctctgaaa atgtttgct ttggagcga gtgcgcggc 
      cgccacttc accaaagct tggagcttt atccaaggt 
      gctaagtcc cttcctgtg cttttagga atccccata 
      accggttat attaatcaa acttatccc aaagcaggg 
      tattatttc agtttcgtg acgacgatc gtcggcgcc 
      agtttaatg tttctggtc ggcacccgc aaagaccac 
      atcaatagc aacaccaat aattgcaac ctgaacggc 
      tgcaatatc gccaacttg aacgagtgc gcttgcaac 
      ctggccctt aattacaac aacatctgc acgcccgac 
      ctgcaaccg tacaatttc tgtcgacaa agcgccaac 
      cactacgag cggcttttc aattacggg cacatggat 
      cgaagcgat ttccaccgg cattcgctg cgccaccat 
      tacctgccg aaaagtctc agctacgcc gccaacatc 
      gacagatcg ccctaccac caatatccg gtgcattat 
      cacgattac gtgcgactg accaacccc aaaagcagg 
      aacatgctg cggcccagc aaaagtgtt cctgaagga 
      ctcgcgcga tgggattat tgcggctcg tacaggagg 
      ccgataatt aggaacgtt caagttatc gaacaaatc 
      accacttcg gtgtaa
    mRNA sequence
    • >TC006428 RA
      gtccgctct taaatcatg gctagtgtt tccacgtcg 
      ttttttatt ccagaccgc agcacgcct ctccctcgg 
      ccgccggaa gacgaagat tcgtttcct gttatttgc 
      gaccgagaa ttgcattca ttgcatcga aacgtgccc 
      gcacttcga cggagggac gtcgatacg gccactatt 
      aagcagcac tactaccct gaaggagga tggggttgg 
      ttggtgtgc ggagctgcc ttcttagcc caccttctc 
      acctccggc ttccaattg gcgttcgga ctgctgtat 
      ttgtatacc atgaagttc ctcattagg aaaaatcaa 
      gacaaagat ttctacgtg atggcaact gcttggttg 
      ggagctttg tgcctgtgt atctctttt ctgaccgct 
      ccattaatt gtggccgtt tgccgaaga aagtccacg 
      agattggcg gccgcgttc gggggcctt gtgacggct 
      ctggcgtgc ctttttacg tcttttgcc gtacagttc 
      caccaggca ctgctcagc tacgggctg gtgctgggc 
      ctgggcgcc ggagtcgtc cgggagact tccgggctg 
      atgctgggc cactatttc aagcggagg agagagttc 
      gtggaaatg gtggcgcaa gccggagcc ggggtcggg 
      atcgccttg ttttcggtg ctttataaa gaagcagtg 
      gggaaactg ggatggaga ttaggcttg caagccatc 
      accggttta ctctccctc gcatttttt ctccctgtt 
      gtctaccga tcggccaat ttgtaccac ccgcagcga 
      cgtgccatt acacattta aaaaatcaa cgcaaaaaa 
      atgaaagaa aagaagacg cacgccaaa gctaagaaa 
      ccgccaatg ttcgatttc acgccgctg aaaagtcgc 
      ggattgcgg gtattgatg gtggcttgc gctttcgct 
      gcgttagga atttacgcc cccgttttc taccttgtg 
      taccagggg tataaggag ggattagaa gacagtgct 
      ttagttctc ttacaaact ttcatgggg tttgcgtca 
      gctttaggc tgcgtcgga tttgggctg gtgactgtg 
      aggcccagc tcgcagtgc ttaatttcc aggcaatat 
      ttgtgtcaa gcggccatg gtagggatc ggagtgtct 
      cttctggcg ctaagtgcc gttcaggga taccacggg 
      tacgttttg tttgtctgg ctgtatggc acttgtctc 
      gggggctac acctactct ctgaaaatg tttgctttg 
      gagcgagtg cgcggccgc cacttcacc aaagcttgg 
      agctttatc caaggtgct aagtccctt cctgtgctt 
      ttaggaatc cccataacc ggttatatt aatcaaact 
      tatcccaaa gcagggtat tatttcagt ttcgtgacg 
      acgatcgtc ggcgccagt ttaatgttt ctggtcggc 
      acccgcaaa gaccacatc aatagcaac accaataat 
      tgcaacctg aacggctgc aatatcgcc aacttgaac 
      gagtgcgct tgcaacctg gcccttaat tacaacaac 
      atctgcacg cccgacctg caaccgtac aatttctgt 
      cgacaaagc gccaaccac tacgagcgg cttttcaat 
      tacgggcac atggatcga agcgatttc caccggcat 
      tcgctgcgc caccattac ctgccgaaa agtctcagc 
      tacgccgcc aacatcgac agatcgccc taccaccaa 
      tatccggtg cattatcac gattacgtg cgactgacc 
      aaccccaaa agcaggaac atgctgcgg cccagcaaa 
      agtgttcct gaaggactc gcgcgatgg gattattgc 
      ggctcgtac aggaggccg ataattagg aacgttcaa 
      gttatcgaa caaatcacc acttcggtg taacgtaac 
      ggttccggt gctaattct agtcatttt agtcactcg 
      ttcgtggat ttgtgagga gtagattat ccaatttag 
      attactttg tacctaaaa agcgaaact tcggtggct 
      ggaaacgag tgttcttta atgtatttc aaattcttg 
      ccttgcgtt tgtgctaaa agcgactcg atttaagac 
      gaccagaca tatctataa tccactggc cccgagttt 
      taaaattta aaggacacg ttattttat tatttagtt 
      accctcatt ttaaaaatt cgggtcact tttaataca 
      agcaaaagt caatcgtct tgactaata tttaaactg 
      ttgaccaga ttttatagc aatacacta tggttgtga 
      ccactttat gacgaatct agacgtact atagtagaa 
      tttatcgtg ggaacaaaa tacacttac tctagaaca 
      aacattagc gaaacatta aagttgaat agaataatt 
      aattgtcgc tttatattt tataaaagc cgattcttt 
      attgattga atttgtaat ctgttgaaa ttttgttat 
      aatttattt gttattaaa ttgattgtt gcattattt 
      atttagatg cgaataaag agattcttt gaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY