| General information | |||
| TC006428 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9058964..9066246 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006428 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04139 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC006428 RA
MASVSTSFF IPDRSTPLP RPPEDEDSF PVICDRELH SLHRNVPAL RRRDVDTAT IKQHYYPEG GWGWLVCGA AFLAHLLTS GFQLAFGLL YLYTMKFLI RKNQDKDFY VMATAWLGA LCLCISFLT APLIVAVCR RKSTRLAAA FGGLVTALA CLFTSFAVQ FHQALLSYG LVLGLGAGV VRETSGLML GHYFKRRRE FVEMVAQAG AGVGIALFS VLYKEAVGK LGWRLGLQA ITGLLSLAF FLPVVYRSA NLYHPQRRA ITHLKNQRK KMKEKKTHA KAKKPPMFD FTPLKSRGL RVLMVACAF AALGIYAPV FYLVYQGYK EGLEDSALV LLQTFMGFA SALGCVGFG LVTVRPSSQ CLISRQYLC QAAMVGIGV SLLALSAVQ GYHGYVLFV WLYGTCLGG YTYSLKMFA LERVRGRHF TKAWSFIQG AKSLPVLLG IPITGYINQ TYPKAGYYF SFVTTIVGA SLMFLVGTR KDHINSNTN NCNLNGCNI ANLNECACN LALNYNNIC TPDLQPYNF CRQSANHYE RLFNYGHMD RSDFHRHSL RHHYLPKSL SYAANIDRS PYHQYPVHY HDYVRLTNP KSRNMLRPS KSVPEGLAR WDYCGSYRR PIIRNVQVI EQITTSV
- >TC006428 RA
atggctagt gtttccacg tcgtttttt attccagac cgcagcacg cctctccct cggccgccg gaagacgaa gattcgttt cctgttatt tgcgaccga gaattgcat tcattgcat cgaaacgtg cccgcactt cgacggagg gacgtcgat acggccact attaagcag cactactac cctgaagga ggatggggt tggttggtg tgcggagct gccttctta gcccacctt ctcacctcc ggcttccaa ttggcgttc ggactgctg tatttgtat accatgaag ttcctcatt aggaaaaat caagacaaa gatttctac gtgatggca actgcttgg ttgggagct ttgtgcctg tgtatctct tttctgacc gctccatta attgtggcc gtttgccga agaaagtcc acgagattg gcggccgcg ttcgggggc cttgtgacg gctctggcg tgccttttt acgtctttt gccgtacag ttccaccag gcactgctc agctacggg ctggtgctg ggcctgggc gccggagtc gtccgggag acttccggg ctgatgctg ggccactat ttcaagcgg aggagagag ttcgtggaa atggtggcg caagccgga gccggggtc gggatcgcc ttgttttcg gtgctttat aaagaagca gtggggaaa ctgggatgg agattaggc ttgcaagcc atcaccggt ttactctcc ctcgcattt tttctccct gttgtctac cgatcggcc aatttgtac cacccgcag cgacgtgcc attacacat ttaaaaaat caacgcaaa aaaatgaaa gaaaagaag acgcacgcc aaagctaag aaaccgcca atgttcgat ttcacgccg ctgaaaagt cgcggattg cgggtattg atggtggct tgcgctttc gctgcgtta ggaatttac gcccccgtt ttctacctt gtgtaccag gggtataag gagggatta gaagacagt gctttagtt ctcttacaa actttcatg gggtttgcg tcagcttta ggctgcgtc ggatttggg ctggtgact gtgaggccc agctcgcag tgcttaatt tccaggcaa tatttgtgt caagcggcc atggtaggg atcggagtg tctcttctg gcgctaagt gccgttcag ggataccac gggtacgtt ttgtttgtc tggctgtat ggcacttgt ctcgggggc tacacctac tctctgaaa atgtttgct ttggagcga gtgcgcggc cgccacttc accaaagct tggagcttt atccaaggt gctaagtcc cttcctgtg cttttagga atccccata accggttat attaatcaa acttatccc aaagcaggg tattatttc agtttcgtg acgacgatc gtcggcgcc agtttaatg tttctggtc ggcacccgc aaagaccac atcaatagc aacaccaat aattgcaac ctgaacggc tgcaatatc gccaacttg aacgagtgc gcttgcaac ctggccctt aattacaac aacatctgc acgcccgac ctgcaaccg tacaatttc tgtcgacaa agcgccaac cactacgag cggcttttc aattacggg cacatggat cgaagcgat ttccaccgg cattcgctg cgccaccat tacctgccg aaaagtctc agctacgcc gccaacatc gacagatcg ccctaccac caatatccg gtgcattat cacgattac gtgcgactg accaacccc aaaagcagg aacatgctg cggcccagc aaaagtgtt cctgaagga ctcgcgcga tgggattat tgcggctcg tacaggagg ccgataatt aggaacgtt caagttatc gaacaaatc accacttcg gtgtaa
- >TC006428 RA
gtccgctct taaatcatg gctagtgtt tccacgtcg ttttttatt ccagaccgc agcacgcct ctccctcgg ccgccggaa gacgaagat tcgtttcct gttatttgc gaccgagaa ttgcattca ttgcatcga aacgtgccc gcacttcga cggagggac gtcgatacg gccactatt aagcagcac tactaccct gaaggagga tggggttgg ttggtgtgc ggagctgcc ttcttagcc caccttctc acctccggc ttccaattg gcgttcgga ctgctgtat ttgtatacc atgaagttc ctcattagg aaaaatcaa gacaaagat ttctacgtg atggcaact gcttggttg ggagctttg tgcctgtgt atctctttt ctgaccgct ccattaatt gtggccgtt tgccgaaga aagtccacg agattggcg gccgcgttc gggggcctt gtgacggct ctggcgtgc ctttttacg tcttttgcc gtacagttc caccaggca ctgctcagc tacgggctg gtgctgggc ctgggcgcc ggagtcgtc cgggagact tccgggctg atgctgggc cactatttc aagcggagg agagagttc gtggaaatg gtggcgcaa gccggagcc ggggtcggg atcgccttg ttttcggtg ctttataaa gaagcagtg gggaaactg ggatggaga ttaggcttg caagccatc accggttta ctctccctc gcatttttt ctccctgtt gtctaccga tcggccaat ttgtaccac ccgcagcga cgtgccatt acacattta aaaaatcaa cgcaaaaaa atgaaagaa aagaagacg cacgccaaa gctaagaaa ccgccaatg ttcgatttc acgccgctg aaaagtcgc ggattgcgg gtattgatg gtggcttgc gctttcgct gcgttagga atttacgcc cccgttttc taccttgtg taccagggg tataaggag ggattagaa gacagtgct ttagttctc ttacaaact ttcatgggg tttgcgtca gctttaggc tgcgtcgga tttgggctg gtgactgtg aggcccagc tcgcagtgc ttaatttcc aggcaatat ttgtgtcaa gcggccatg gtagggatc ggagtgtct cttctggcg ctaagtgcc gttcaggga taccacggg tacgttttg tttgtctgg ctgtatggc acttgtctc gggggctac acctactct ctgaaaatg tttgctttg gagcgagtg cgcggccgc cacttcacc aaagcttgg agctttatc caaggtgct aagtccctt cctgtgctt ttaggaatc cccataacc ggttatatt aatcaaact tatcccaaa gcagggtat tatttcagt ttcgtgacg acgatcgtc ggcgccagt ttaatgttt ctggtcggc acccgcaaa gaccacatc aatagcaac accaataat tgcaacctg aacggctgc aatatcgcc aacttgaac gagtgcgct tgcaacctg gcccttaat tacaacaac atctgcacg cccgacctg caaccgtac aatttctgt cgacaaagc gccaaccac tacgagcgg cttttcaat tacgggcac atggatcga agcgatttc caccggcat tcgctgcgc caccattac ctgccgaaa agtctcagc tacgccgcc aacatcgac agatcgccc taccaccaa tatccggtg cattatcac gattacgtg cgactgacc aaccccaaa agcaggaac atgctgcgg cccagcaaa agtgttcct gaaggactc gcgcgatgg gattattgc ggctcgtac aggaggccg ataattagg aacgttcaa gttatcgaa caaatcacc acttcggtg taacgtaac ggttccggt gctaattct agtcatttt agtcactcg ttcgtggat ttgtgagga gtagattat ccaatttag attactttg tacctaaaa agcgaaact tcggtggct ggaaacgag tgttcttta atgtatttc aaattcttg ccttgcgtt tgtgctaaa agcgactcg atttaagac gaccagaca tatctataa tccactggc cccgagttt taaaattta aaggacacg ttattttat tatttagtt accctcatt ttaaaaatt cgggtcact tttaataca agcaaaagt caatcgtct tgactaata tttaaactg ttgaccaga ttttatagc aatacacta tggttgtga ccactttat gacgaatct agacgtact atagtagaa tttatcgtg ggaacaaaa tacacttac tctagaaca aacattagc gaaacatta aagttgaat agaataatt aattgtcgc tttatattt tataaaagc cgattcttt attgattga atttgtaat ctgttgaaa ttttgttat aatttattt gttattaaa ttgattgtt gcattattt atttagatg cgaataaag agattcttt gaac
