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Gene information
General information
TC006424 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9013194..9014467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006424
    iBeetle screen iB_04138

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006424 RA
      MLLAVAVVL CASSTRGLL GRSGGFPKF PADSCGRRA 
      VPWRPEREP RVVGGEEPP YGAVPWQVE LRLGQRHQC 
      GGALIARRL VLTAAHCWA EGLVAVAGA HGPPGTAPF 
      EQTLRVERA VQHPDFRKL GPYSHDIAV LLLADPGLD 
      FNFLVKPAC FAYDSPPPG TWCEVSGWG ASDPKAPDR 
      LSPVLRSAA VPLLSLETC RKDGIYGGR QQPILDSML 
      CAGHLRGGI DACGGDSGG PLVCERDGR HELTGIVSW 
      GDGCAKKDR PGVYTRVAS FLPWIRDCA RQLGVDYNF
    CDS sequence
    • >TC006424 RA
      atgctcctc gcggtggcc gtcgtgctc tgcgcttct 
      tcgacgcgc ggcctcctg gggcgcagc gggggcttc 
      cccaagttc ccggccgat tcgtgcggg cggcgcgcc 
      gtcccctgg cggccggag cgcgagccg cgcgtggtg 
      ggcggcgag gagccgcct tacggcgcc gtcccctgg 
      caggtggag ctgcggctg gggcagcgc caccagtgt 
      gggggcgcg ctcatcgcc cgccgcctg gtgctcacc 
      gccgcgcac tgctgggcc gaggggctg gtggccgtg 
      gcgggggcg cacgggccc ccggggacg gcgcccttc 
      gagcagacg ctccgggtg gaacgggcc gtgcaacac 
      cccgatttc cgcaagctg gggccctac agccacgac 
      atcgccgtg ctgctgctg gccgatcct ggactcgac 
      ttcaacttc ctggtgaag ccggcgtgt tttgcgtac 
      gattcgccg ccgccgggc acctggtgc gaggtgtct 
      ggatggggg gctagtgac ccgaaggcg ccggatagg 
      ctgtcgccg gttttgagg tcggctgcg gtgccactt 
      ctttccctg gagacctgt cgaaaggat gggatttat 
      ggaggaaga cagcagccc attttggat tcgatgctg 
      tgtgcgggg catttgcga gggggaatt gacgcttgc 
      ggtggggac tctggcggg ccgttggtt tgtgaaaga 
      gatggccga catgaactg acgggaatt gtatcttgg 
      ggtgatgga tgtgccaag aaagataga ccgggagtt 
      tatacaaga gtggcgagc tttttacca tggattaga 
      gactgtgcg aggcagtta ggcgttgat tataacttt 
      taa
    mRNA sequence
    • >TC006424 RA
      ggcacgaaa tcatcgttt cgctttcaa taataacat 
      cctgtaagt gtgtcacag gggaaagtg cttctgggc 
      gtcgcggcc aaggtgaca tgggcatcg atgtgggcc 
      actgcgcaa ttaaaaagt caccgtgtg cgggatcct 
      atgacataa taaccgtaa caacaatcg ggaggtcgc 
      gccgcttcg gcccagttt cccaagtgc accagaagt 
      ttccgcaag cctcggcgg cgatttcgg tggtgggcg 
      tacaggcca taaaacctc acccgctgg cccggatcc 
      ccatacgtg cgcgatgct cctcgcggt ggccgtcgt 
      gctctgcgc ttcttcgac gcgcggcct cctggggcg 
      cagcggggg cttccccaa gttcccggc cgattcgtg 
      cgggcggcg cgccgtccc ctggcggcc ggagcgcga 
      gccgcgcgt ggtgggcgg cgaggagcc gccttacgg 
      cgccgtccc ctggcaggt ggagctgcg gctggggca 
      gcgccacca gtgtggggg cgcgctcat cgcccgccg 
      cctggtgct caccgccgc gcactgctg ggccgaggg 
      gctggtggc cgtggcggg ggcgcacgg gcccccggg 
      gacggcgcc cttcgagca gacgctccg ggtggaacg 
      ggccgtgca acaccccga tttccgcaa gctggggcc 
      ctacagcca cgacatcgc cgtgctgct gctggccga 
      tcctggact cgacttcaa cttcctggt gaagccggc 
      gtgttttgc gtacgattc gccgccgcc gggcacctg 
      gtgcgaggt gtctggatg gggggctag tgacccgaa 
      ggcgccgga taggctgtc gccggtttt gaggtcggc 
      tgcggtgcc acttctttc cctggagac ctgtcgaaa 
      ggatgggat ttatggagg aagacagca gcccatttt 
      ggattcgat gctgtgtgc ggggcattt gcgaggggg 
      aattgacgc ttgcggtgg ggactctgg cgggccgtt 
      ggtttgtga aagagatgg ccgacatga actgacggg 
      aattgtatc ttggggtga tggatgtgc caagaaaga 
      tagaccggg agtttatac aagagtggc gagcttttt 
      accatggat tagagactg tgcgaggca gttaggcgt 
      tgattataa cttttaagg aaatttttc aaaatatga 
      gtagcacat tgttacttg tgattacga atttatttt 
      agttttaat cattgtgta aatacaact ttaaataaa 
      gaatatttc attct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY