Status
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Gene information
General information
TC034081
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006423
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9002784..9008842
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC034081
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04137

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      Adult morphology
      abdominal odoriferous glands size increased - Number of animals showing the phenotype: 4 (22 dpi)
      Days post injection



      odoriferous glands size increased - Number of animals showing the phenotype: 6 (41 dpi)
      Days post injection



      Fertility
      egg production: number <50% of expected -
      ovary structure without detectable defects - Number of animals showing the phenotype: 4
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034081 RA
        MSQIKYTSH FCFLFPDCL HLQLYRDSK DRYKQGQTK 
        ASLSLQHFL GLETGFTLD KESNTVAIL CQDVVVVLA 
        FDNRERLMQ WQVKLSTHL GDGPHFLVL VSSTPAKSR 
        LPPGPARLH VQERGFCLT TGVPPRVAG EWLISNLRR 
        YGVVEGRFC FEGGSRCGK GEGLHVLLT DQWEEITST 
        LKVAAVGQL HASRRRPVS RNMSVMDSP RRPALRSEC 
        RATMELSDS SIISRADSP YTDLESNYG CGDSISNDG 
        WGVPPIERC MSCISKLGA LSRSSTATV TMATPCWEH 
        TCDRHSISS GSDSSGWSQ AVTKPPARP PKPNSGPLT 
        SPSKKPPAP APSCYDNYD IPKIPYPVE SKLDEHYDT 
        PRKLKECLF DTVHKPCGC IIRVRQPEP ERPCVCQRL 
        MSCWSGDED INALYATVD RSKKCQRNQ PTAANYANI 
        APMSAPTVP TSSNYENME FAQTLKLYE NSKEIVEKA 
        RALCSKCGH AQDDYLMME PSASKPAPH PGYIPMSPA 
        VKQRLGKAL SNSNPNLAP ALDRSNKPS GSAMLHANV 
        YQRKQLLDS TDNLDLKRR RSNSADSSR CEETETSPC 
        HCVQSVAVR RSLSVPCKS NRDSSSSND SGVFELPRK 
        AQLCTVSTL PRRSKSSDP LRDLSFQFN KAEGKSASA 
        EAEVPVCPS GSTSSGTSD MSDYFETLS LSSHSSNEA 
        PPPSCTLRP RSGNEYLSL QRLIAPVPE ERHN
      CDS sequence
      • >TC034081 RA
        atgtcccaa attaagtac acaagtcat ttctgtttt 
        ctttttcca gattgcctc cacttgcaa ctttatcgc 
        gattcaaag gatcgctac aagcaaggc cagactaaa 
        gcctcgctt tccctccaa catttcttg ggtctggaa 
        actggattc acattagat aaagaatcg aacaccgta 
        gccattctc tgtcaggac gtcgtagtc gtcctggct 
        ttcgataac agagaacgc ttgatgcaa tggcaagtc 
        aagctgagc acacattta ggggacggc ccgcatttt 
        ctcgtgctt gtatcgtca actccggca aagtcacga 
        ttacctcca ggtcccgca agactgcac gtccaagaa 
        cggggtttt tgtttgact actggagtt ccgccgcga 
        gtcgcgggc gaatggctg attagcaac ttgcggagg 
        tatggggtg gtggaggga cggttctgc ttcgagggc 
        ggctcgagg tgcgggaaa ggagaaggg ctgcacgtg 
        ctactaacc gaccaatgg gaggaaatt acttccacg 
        ttaaaggtc gcggccgtt ggccagctg cacgcgtcc 
        aggaggcgg ccagtctcg agaaacatg tcagttatg 
        gatagcccg cgccggccc gccctgcgg tctgagtgc 
        cgggccacg atggagttg agcgattcc tcgattata 
        tctcgtgct gattctcct tacaccgat ttggagagc 
        aattatggt tgtggtgat agtatatcg aacgatggt 
        tggggggtg ccgccgatt gagcgttgt atgagttgt 
        atcagcaaa cttggtgcg ttatcgcga tcatcaacg 
        gcgactgtg acaatggca acgccgtgt tgggaacac 
        acttgtgac cgacattcg attagcagt ggttccgat 
        agtagtggt tggagccaa gcggtcact aaaccaccg 
        gcacggccc ccgaaacca aattctggg cctttaact 
        tctcccagt aagaagccg ccagcgccg gctccatct 
        tgctatgac aattacgac attcccaaa atcccatat 
        cccgtggag tcaaaattg gacgagcat tacgacacg 
        cctcgcaag ctgaaagaa tgtttattc gacacagtc 
        cacaaaccg tgcggttgt ataatccgc gtccgccag 
        cccgagccg gaaaggccg tgcgtttgt caacgtctc 
        atgtcgtgc tggtctgga gatgaagac ataaacgcc 
        ctttatgcc acagttgac aggagtaaa aaatgtcaa 
        cgcaatcaa cccacagct gccaactac gccaacata 
        gctcccatg tcggccccc actgttcct acttcttcc 
        aattacgaa aacatggaa tttgctcaa actctcaaa 
        ctgtacgaa aacagcaaa gaaatcgtg gaaaaagcg 
        cgcgctctg tgcagcaaa tgcggccac gctcaagac 
        gactacctc atgatggag ccctccgcc agcaagccg 
        gcgccgcac cctggctac atccccatg tccccggct 
        gtgaagcag cgcctgggg aaggcgctc tccaacagc 
        aaccccaac ctggcgccg gccctggac cgctccaac 
        aagccctcg ggctcggcc atgctgcac gcgaacgtc 
        tatcaacga aagcaactg ctcgactcg actgataat 
        ctagatttg aagcgacga cgatcaaat tcggctgat 
        tccagccgc tgcgaagag actgaaact tctccgtgt 
        cattgcgtc cagtccgta gctgtgaga aggtccctg 
        tccgttccg tgcaaatcc aatcgggac tcgtcgagt 
        tccaacgat tccggagtc tttgagtta cctcgcaag 
        gcgcaatta tgcaccgtc tccacttta cctcgacgt 
        tcgaaatct tcagacccg ctccgggat ttgagcttt 
        caatttaat aaggctgaa ggaaagtcc gccagtgcc 
        gaggcggag gttccggtc tgtccgtcg ggatcgacc 
        agcagtggt acttccgac atgtccgat tacttcgaa 
        acgctgtcc ttgtcctca cacagctcc aacgaagcg 
        ccgccgccg agctgcacg ctcagaccc cgctcgggg 
        aacgaatat ctcagtctg cagaggctc atcgcaccc 
        gttcccgag gaacggcat aactag
      mRNA sequence
      • >TC034081 RA
        acggaataa aaagtgtga aacttgggt aacaaggag 
        ttacttatt caactccta acgagtgtg tgggtcgtt 
        ttccgtctg ggtgatgaa taaacagga acaataatt 
        tgagaacag ttcggtgtc gacttaccc aaaggatgt 
        ttcaaagag ccgccgcca tcgcttttg aagttggat 
        cgcttcaat taggtcgat tctgttgaa cttcggcac 
        gttgatgtt gcattttat tagtctcat aaggctatt 
        gcggtgagc caaatcgag taacaaagt ctcgtggac 
        acgcacgca aaacttcgc ctattctgg ccatctcga 
        aatttattt ctttgatgt caacttgcg gtcaatgga 
        gccccaaac ctgcatctt tttcccaat tctgcgata 
        tacgcatcc acaaagcga atataaaac gttcaggca 
        acgtaatcc tgcccagga aaggggaga ttaaccggg 
        gagggtgcg taatttact tctctttac gagggcggc 
        ctctgcgac gaatccaac tcaaattcc gtttgttgc 
        ggaaaccaa aggataatt aaagaatcg tttctttgt 
        acaagaaag aatccgaca tcaaagttt tcagcggtc 
        agcgagctt tttcagcga cataacagc catagaaag 
        agcgagttt gacgggatt ggtttcacg cccaataaa 
        ataaaggaa ggcaaatga tccgttcgg agcagacgg 
        ggatccgtt ccaacactt gactgttga aagaaaata 
        ttgcccaaa aaatccgaa ctggatgaa ctaccccgg 
        cttcgaatt tccccggca aaaaaccaa cagatcatg 
        agaaaattt tgtgttggt taaatgcaa taatcaatt 
        tattactga gtaatatga cgtgaggca cagatggcg 
        ttaccatta atacgagcc gtgggaatc atcaatctt 
        gcggagcgt catcccgcg cggtggccg gttaaggtt 
        tagcacaga gatagaaat atttcaacc gttcagctt 
        atttaaata ctcctccct gcaaaaatg aaattgctc 
        taatcggca cgtgttttg aaattatcg ggaagcgtg 
        cccgatatt catcatctg atacgaaaa actgacaaa 
        tgacgccga ccgttgtta atgggatca gtcagttgg 
        ggctgaaac aattcactt ccgcattta ctagcccat 
        tattattaa tagactaca aaactggct aaactttcc 
        gggctagga taataaact tccggattg cgcgtcttg 
        aaaggttcg cttttattg tgatagtac tcggtctat 
        ttttcggcg agaatattt tcaaaccaa ctggaatca 
        acccgacca taatcctta tcgatggct gcagatccg 
        ctctgtggc gtcgggctg atacgacag ccacgaggc 
        acaacttat tctttcgta ttaccataa gctggaata 
        acaggatcc aaggacgca taattcgcc cattgtttg 
        ccgataagt cttaattgt gcaaagcaa caatatagc 
        cgtggcttt tgtaattga cgcgagaaa agaagccaa 
        gattgcagc tggatcgaa gaataaact ccgaatgct 
        tagcgaagt ggcatccga accccatgc aatttcatg 
        gaccgctta ctctggccg gatttacac gttattgtt 
        aaagttttg gttagggta aagtcgaat taaacttat 
        acgtggtga accactcta aaaaatagc actcaacac 
        ctgaacaaa gccggttaa cggctcaca ctgatggct 
        ctcgaagcc actgcattt gtaatcgta tcggtgaaa 
        attgattat gtggtaaaa atgtcccaa attaagtac 
        acaagtcat ttctgtttt ctttttcca gattgcctc 
        cacttgcaa ctttatcgc gattcaaag gatcgctac 
        aagcaaggc cagactaaa gcctcgctt tccctccaa 
        catttcttg ggtctggaa actggattc acattagat 
        aaagaatcg aacaccgta gccattctc tgtcaggac 
        gtcgtagtc gtcctggct ttcgataac agagaacgc 
        ttgatgcaa tggcaagtc aagctgagc acacattta 
        ggggacggc ccgcatttt ctcgtgctt gtatcgtca 
        actccggca aagtcacga ttacctcca ggtcccgca 
        agactgcac gtccaagaa cggggtttt tgtttgact 
        actggagtt ccgccgcga gtcgcgggc gaatggctg 
        attagcaac ttgcggagg tatggggtg gtggaggga 
        cggttctgc ttcgagggc ggctcgagg tgcgggaaa 
        ggagaaggg ctgcacgtg ctactaacc gaccaatgg 
        gaggaaatt acttccacg ttaaaggtc gcggccgtt 
        ggccagctg cacgcgtcc aggaggcgg ccagtctcg 
        agaaacatg tcagttatg gatagcccg cgccggccc 
        gccctgcgg tctgagtgc cgggccacg atggagttg 
        agcgattcc tcgattata tctcgtgct gattctcct 
        tacaccgat ttggagagc aattatggt tgtggtgat 
        agtatatcg aacgatggt tggggggtg ccgccgatt 
        gagcgttgt atgagttgt atcagcaaa cttggtgcg 
        ttatcgcga tcatcaacg gcgactgtg acaatggca 
        acgccgtgt tgggaacac acttgtgac cgacattcg 
        attagcagt ggttccgat agtagtggt tggagccaa 
        gcggtcact aaaccaccg gcacggccc ccgaaacca 
        aattctggg cctttaact tctcccagt aagaagccg 
        ccagcgccg gctccatct tgctatgac aattacgac 
        attcccaaa atcccatat cccgtggag tcaaaattg 
        gacgagcat tacgacacg cctcgcaag ctgaaagaa 
        tgtttattc gacacagtc cacaaaccg tgcggttgt 
        ataatccgc gtccgccag cccgagccg gaaaggccg 
        tgcgtttgt caacgtctc atgtcgtgc tggtctgga 
        gatgaagac ataaacgcc ctttatgcc acagttgac 
        aggagtaaa aaatgtcaa cgcaatcaa cccacagct 
        gccaactac gccaacata gctcccatg tcggccccc 
        actgttcct acttcttcc aattacgaa aacatggaa 
        tttgctcaa actctcaaa ctgtacgaa aacagcaaa 
        gaaatcgtg gaaaaagcg cgcgctctg tgcagcaaa 
        tgcggccac gctcaagac gactacctc atgatggag 
        ccctccgcc agcaagccg gcgccgcac cctggctac 
        atccccatg tccccggct gtgaagcag cgcctgggg 
        aaggcgctc tccaacagc aaccccaac ctggcgccg 
        gccctggac cgctccaac aagccctcg ggctcggcc 
        atgctgcac gcgaacgtc tatcaacga aagcaactg 
        ctcgactcg actgataat ctagatttg aagcgacga 
        cgatcaaat tcggctgat tccagccgc tgcgaagag 
        actgaaact tctccgtgt cattgcgtc cagtccgta 
        gctgtgaga aggtccctg tccgttccg tgcaaatcc 
        aatcgggac tcgtcgagt tccaacgat tccggagtc 
        tttgagtta cctcgcaag gcgcaatta tgcaccgtc 
        tccacttta cctcgacgt tcgaaatct tcagacccg 
        ctccgggat ttgagcttt caatttaat aaggctgaa 
        ggaaagtcc gccagtgcc gaggcggag gttccggtc 
        tgtccgtcg ggatcgacc agcagtggt acttccgac 
        atgtccgat tacttcgaa acgctgtcc ttgtcctca 
        cacagctcc aacgaagcg ccgccgccg agctgcacg 
        ctcagaccc cgctcgggg aacgaatat ctcagtctg 
        cagaggctc atcgcaccc gttcccgag gaacggcat 
        aactagtct ataaatagg aagcaataa atattaaca 
        gtatctagt acaacatta tatgattaa actatacat 
        gtaatcatt ttctgtata aaattatga ctttataat 
        aaattattc attgaaca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY