Status
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Gene information
General information
TC034076 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8669948..8698834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034076
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04136

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034076 RA
      MTEGSEDEL EVSEQPEDT HMDAVMVPP QHETFTDFS 
      SNNNLDDPP SKLSLKPAD QLFCDETLK AFNGATDLV 
      YSRCREAIP ASGVVPCEI ELKDGVFAK GVIPKGTRY 
      GPFKGKWAG VPQDVRFAW EVVAGSGVR GWLDGSGES 
      QNWLKLIRS CDKSSANMR YYLQGGQLW YETCVDIPN 
      GAELIMGPR EPLNLQDMF SDSVSADEK SDRETASQH 
      SGTVDERED DDEDESETR CCVCDQPFT DVDKLDDHL 
      IVKHNYRKD EYRCDLCPK AYSHRPCLI KHRSVSHGE 
      HRKYHCENC PKVFTDPSN LQRHIRIHH VGARSHACP 
      ECGKTFATS SGLKQHTHI HSSVKPFQC EVCFKAYTQ 
      FSNLCRHKR MHADCRMQI KCVKCGQSF STVTSLSKH 
      KRFCDSTTP TPSLSSQQN LPMGSNNPF SMYNTPACP 
      LPFFPTHFS PYPPIFPPS GHHPSFINP LLFNPIPKL 
      KSEEEQDGP KKLRLVSPD RIDLLRPGL QNFSSKPFH 
      DMIQQQAQG KMKDRYACK FCGKVFPRS ANLTRHLRT 
      HTGEQPYKC RYCERSFSI SSNLQRHVR NIHNKEKPF 
      KCPLCERCF GQQTNLDRH LKKHEADDG SGGVAVADS 
      PGSSNENER EDACFDEIR SFMGKVTYA GGDPYNQTR 
      IYTPPSHSV IDVVGKDEE DSETYSEEA PLEEFAANK 
      IEDEVLNNN DQTIKVST
    CDS sequence
    • >TC034076 RA
      atgacagaa ggcagcgaa gatgaacta gaagtctcc 
      gagcagcct gaagacacc cacatggac gcggtaatg 
      gtcccgccc cagcacgaa accttcacc gacttctcc 
      tccaacaac aacctcgac gaccctccc agcaagctc 
      agcctcaag cccgccgac caactcttc tgtgatgaa 
      accctcaag gccttcaac ggcgccaca gacctggtg 
      tactccaga tgccgcgaa gcgatcccg gcctccggg 
      gtcgtcccc tgcgagatc gagctcaag gacggggtc 
      ttcgccaag ggcgtcatc cccaagggc acccgctac 
      ggcccgttc aagggcaag tgggccggc gtcccgcag 
      gatgtgcgc ttcgcctgg gaggtcgtc gccggctcc 
      ggagtccgg ggatggctg gacggctcc ggggagagc 
      cagaactgg ctcaaactg atccgaagt tgcgataaa 
      agctcagcg aatatgcgg tattatctc caaggagga 
      caactgtgg tacgaaacc tgcgtggac attcccaac 
      ggggccgag ctgatcatg ggccctcgg gagccgctc 
      aacctgcag gacatgttc agcgactcg gtctcggcc 
      gacgaaaag tccgatcgg gaaacagcg tcgcagcac 
      agtggtacg gtggacgag cgtgaagac gacgacgaa 
      gacgagtcc gaaacgcgc tgttgcgtg tgcgaccag 
      cccttcacc gacgtagat aaattagac gaccatctc 
      atcgtcaag cacaactac cgcaaggat gagtaccgg 
      tgcgacctc tgccctaaa gcctactcc catcgtcct 
      tgcctcata aagcaccgg tcggtgagc cacggcgag 
      caccggaag taccattgc gagaactgc cccaaggta 
      tttacagac cccagcaat ctccagaga cacataaga 
      atccaccac gtgggtgcc cggtcccat gcgtgccca 
      gaatgcgga aagactttc gcaacatcg tcgggactt 
      aaacaacac acgcatatc cactcgagt gtgaagccc 
      tttcagtgc gaagtgtgc tttaaggcg tacacgcaa 
      ttctcgaac ttgtgccgc cacaaacgg atgcatgcc 
      gactgtcgc atgcaaata aaatgcgtc aagtgtggg 
      cagtcgttc tcgacggtg acttctcta tcgaaacat 
      aaacgattt tgtgattcc acaacacca actccttcg 
      ctaagctcc cagcagaat ttgcccatg gggagtaat 
      aacccgttc tcgatgtat aacacgccg gcttgccct 
      ctcccgttt ttcccaact cacttttcc ccctacccg 
      cccattttc ccaccgtcg ggacaccac ccatcgttc 
      attaacccg ctcctgttc aaccccatc ccgaaattg 
      aagagtgag gaagagcag gacgggccg aaaaaactg 
      agattggtg agtccggac cggattgac ttgctgcgg 
      cctgggctc cagaacttc agctcgaag ccgtttcat 
      gacatgatc cagcagcaa gcccagggg aagatgaag 
      gaccggtac gcttgcaaa ttctgcggc aaggtgttc 
      ccccggtcc gcgaacctg acccggcac ttgcggacg 
      cacacgggg gagcagccc tacaagtgc cgctattgt 
      gagcgctct ttcagcatc tcgagcaat ctgcagcgg 
      cacgtgagg aacattcac aacaaagag aagcccttc 
      aagtgtccg ctgtgcgag aggtgtttc ggccaacaa 
      accaattta gatcggcat ctgaagaag cacgaggcg 
      gacgacggg agcggagga gtggccgtg gcggactcg 
      cccgggagc agcaacgag aacgagcgg gaggacgcg 
      tgttttgac gagatccgc tccttcatg gggaaggtg 
      acgtacgct gggggcgac ccctacaac cagacccgg 
      atctacacg ccgccctcg cacagcgtg atcgacgtg 
      gtggggaag gacgaggag gactcggag acgtactcg 
      gaggaggcg cccctggag gagttcgca gcgaataag 
      atcgaggac gaagtgctg aacaacaac gatcagact 
      ataaaagtt tccacttag
    mRNA sequence
    • >TC034076 RA
      tgacaggag agtcccgga tcctggagc atcctgaaa 
      gtcagtgtg ttgtcgaac ttaatagag ttgactact 
      gttacgtaa cgaaatttt taactaagt ttgactttc 
      aaattgatt tggcgtgat gaagtggta gttgaccga 
      tttttgtga tagcggact tgcgttatg tttgatgat 
      tgccaaaat tatgacaga aggcagcga agatgaact 
      agaagtctc cgagcagcc tgaagacac ccacatgga 
      cgcggtaat ggtcccgcc ccagcacga aaccttcac 
      cgacttctc ctccaacaa caacctcga cgaccctcc 
      cagcaagct cagcctcaa gcccgccga ccaactctt 
      ctgtgatga aaccctcaa ggccttcaa cggcgccac 
      agacctggt gtactccag atgccgcga agcgatccc 
      ggcctccgg ggtcgtccc ctgcgagat cgagctcaa 
      ggacggggt cttcgccaa gggcgtcat ccccaaggg 
      cacccgcta cggcccgtt caagggcaa gtgggccgg 
      cgtcccgca ggatgtgcg cttcgcctg ggaggtcgt 
      cgccggctc cggagtccg gggatggct ggacggctc 
      cggggagag ccagaactg gctcaaact gatccgaag 
      ttgcgataa aagctcagc gaatatgcg gtattatct 
      ccaaggagg acaactgtg gtacgaaac ctgcgtgga 
      cattcccaa cggggccga gctgatcat gggccctcg 
      ggagccgct caacctgca ggacatgtt cagcgactc 
      ggtctcggc cgacgaaaa gtccgatcg ggaaacagc 
      gtcgcagca cagtggtac ggtggacga gcgtgaaga 
      cgacgacga agacgagtc cgaaacgcg ctgttgcgt 
      gtgcgacca gcccttcac cgacgtaga taaattaga 
      cgaccatct catcgtcaa gcacaacta ccgcaagga 
      tgagtaccg gtgcgacct ctgccctaa agcctactc 
      ccatcgtcc ttgcctcat aaagcaccg gtcggtgag 
      ccacggcga gcaccggaa gtaccattg cgagaactg 
      ccccaaggt atttacaga ccccagcaa tctccagag 
      acacataag aatccacca cgtgggtgc ccggtccca 
      tgcgtgccc agaatgcgg aaagacttt cgcaacatc 
      gtcgggact taaacaaca cacgcatat ccactcgag 
      tgtgaagcc ctttcagtg cgaagtgtg ctttaaggc 
      gtacacgca attctcgaa cttgtgccg ccacaaacg 
      gatgcatgc cgactgtcg catgcaaat aaaatgcgt 
      caagtgtgg gcagtcgtt ctcgacggt gacttctct 
      atcgaaaca taaacgatt ttgtgattc cacaacacc 
      aactccttc gctaagctc ccagcagaa tttgcccat 
      ggggagtaa taacccgtt ctcgatgta taacacgcc 
      ggcttgccc tctcccgtt tttcccaac tcacttttc 
      cccctaccc gcccatttt cccaccgtc gggacacca 
      cccatcgtt cattaaccc gctcctgtt caaccccat 
      cccgaaatt gaagagtga ggaagagca ggacgggcc 
      gaaaaaact gagattggt gagtccgga ccggattga 
      cttgctgcg gcctgggct ccagaactt cagctcgaa 
      gccgtttca tgacatgat ccagcagca agcccaggg 
      gaagatgaa ggaccggta cgcttgcaa attctgcgg 
      caaggtgtt cccccggtc cgcgaacct gacccggca 
      cttgcggac gcacacggg ggagcagcc ctacaagtg 
      ccgctattg tgagcgctc tttcagcat ctcgagcaa 
      tctgcagcg gcacgtgag gaacattca caacaaaga 
      gaagccctt caagtgtcc gctgtgcga gaggtgttt 
      cggccaaca aaccaattt agatcggca tctgaagaa 
      gcacgaggc ggacgacgg gagcggagg agtggccgt 
      ggcggactc gcccgggag cagcaacga gaacgagcg 
      ggaggacgc gtgttttga cgagatccg ctccttcat 
      ggggaaggt gacgtacgc tgggggcga cccctacaa 
      ccagacccg gatctacac gccgccctc gcacagcgt 
      gatcgacgt ggtggggaa ggacgagga ggactcgga 
      gacgtactc ggaggaggc gcccctgga ggagttcgc 
      agcgaataa gatcgagga cgaagtgct gaacaacaa 
      cgatcagac tataaaagt ttccactta gtgataagc 
      ttgactcaa ataactgtg ttatgcatc aacgtaatt 
      taggtgtag ttagtgtta tttattgta catacggag 
      acttgttcg agtcaaaaa ttataactt tttaaagtt 
      cctacacga attttgtca atttgtctg aacactttg 
      cacggaatt aagcaattg tagcacttt tcttttcac 
      actccttca gatgtaata attgtaagt tgtattgta 
      catagagtg gtgcatttg tgtgactcc cgttgttgt 
      ttgtacata tctaggaga attttttgt tgcttcagt 
      tcgattaac aaaaatgta ccataggga aattatttc 
      actcatagg tgagccaat tcagccaaa gctacgcta 
      attttgtac aaaacaatc ttcacttat tagtaataa 
      acgtactag tttatagtt tgttgatag tttattata 
      ctcatttaa atcactcat atactagac acctatatc 
      acaatgtaa aataaatta ataaattta ttaccatga 
      c
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