General information | |||
TC031278
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 8468958..8481925 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031278 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04133 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04134 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC031278 RA
MGNCLACFK EPNSTALDN SPNCRKEEM PEAQLYPAP SHVPASSAP TETILSNGN HLTALPEPA NNRFYPRKP HSKSAPAMG LTDSKPSDA KLNALFDQY KDNAEDTIL AEGIEQLCR DLQISPDDF RILVLAWKL NAEQMCRFT RSEFVTGLK AMRADSVKG IQGRLPELV AEVGQDVEQ FKDLYRFTF RFGLDSAAG QRILPTDMA VVLWRLVFT MREPPILAR WLSFLESHQ SVRGIPRDT WNMFLNFAE AVGDDLSCY DDNEAWPSL FDDFVEPSK HYLLSPTHK METSRAIVV KKFGSYDVL SVEHFALPP LEGNVEVKV EYGGVNFAD LYTRQGLMH NKKLPFVLG MECVGTIVK IGAENTQLK VGQHVICYD YHCGMYRDV VRVTPDKCY PLPDHVTWE EGAAIFVNY LTAYFSIIE LGNLRQNET VLILSCGGG VGWAGTQLA KTVPGVTVV GTTAPFKHP EVKANGVDI TLTLDENFQ KEITKECPQ GFDLILSNY AGPLYGFLK TLLKPLGRI VLIGANNLI QNEQKLSIF TLLKGWLTT KNVDLEELI TRNRVAAGL HLGTLIEKD PGRVRDALG VIFKMMEEK KLKVKIDSV HPMEKIVDA TKLLAERKN VGKDCRLGV QLCVSLNRA PCGATVAST GTVRFLRAC HLCFFWNHL PSWTHLARL DIRHTMADI DDTHFETGD SGASATYPM QCSALRKNG FVMLKSRPC KIVEMSTSK TGKHGHAKV HLVGIDIFT GKKYEDICP STHNMDVPH VKREDYQLT DISDDGYLS LMSDNGDLR EDLKVPEGD VGAQLRAEY DAGKDILCT VLKSCGEEV VIAVKTNTA LDK
- >TC031278 RA
atgggcaat tgcctggcg tgcttcaag gagcccaac agtacggcc ctggacaac tcgcccaat tgccgtaaa gaagaaatg cccgaagcg cagctgtat ccggctccg agtcacgtg ccagcctcc tcggcccca accgagacg atcctgagc aacggcaac cacctaaca gcccttccg gaacccgcc aacaaccgt ttctaccct cgcaagccc cattcgaag tcggcgccg gcgatgggt ctcaccgat agcaaacca tccgacgcg aaactaaac gcacttttc gaccagtac aaggacaac gcggaggac accatcctc gcagagggc atcgaacag ttgtgcaga gacctgcag atctcgccc gacgatttt cggattttg gtactggcg tggaagctc aacgccgag cagatgtgt cggttcacc aggtccgag tttgtcacg gggttgaag gccatgcgg gccgactcg gtcaagggg atccagggc cggctgccc gagctcgtc gcggaggtc ggccaggac gtggagcag tttaaggat ttgtacagg tttaccttc cggttcggc ctggattct gcggcgggg cagcgaatc ctgccgacg gacatggcg gtggtgctg tggcggctg gtgttcaca atgcgggaa cccccgatc ctcgcgcgc tggctgtcg ttcctcgag agtcaccag tcggttcgg gggattccc cgcgacacg tggaacatg tttttgaat ttcgccgaa gccgtgggc gacgacttg agttgttac gacgacaac gaggcgtgg ccgagcctg ttcgacgac ttcgtcgag ccaagcaaa cactacttg ttgagccct acccataaa atggaaacg agcagagcc atcgtggtg aaaaaattc ggcagttac gacgtcctt agcgtggaa cactttgcc ttgcctcct ttggagggc aatgtcgaa gtgaaagtg gaatatggg ggcgtaaac tttgccgat ttgtacacc cggcaaggt ttgatgcac aacaaaaaa ctgccgttt gttttgggg atggaatgt gtgggcaca attgtgaaa atcggggcc gaaaacaca caactcaaa gtgggccaa catgtcatc tgttatgat tatcactgt gggatgtac cgggacgta gtcagggta acccccgac aaatgctac cctctaccc gaccacgtg acttgggag gagggtgct gcgattttc gtcaattac ttaaccgcc tacttttcc attatcgag ctcgggaat ttgcggcaa aatgagaca gttttgata ctctcatgc ggggggggt gtggggtgg gcgggcacc cagctggcc aaaaccgtc cccggggtc acagtggtg ggcaccaca gcccccttc aagcacccc gaagtcaaa gccaacggc gtcgatatc accctaact ttggacgag aatttccaa aaggaaata acaaaagag tgtccccaa ggctttgat ttaattctg tctaattac gccggcccg ctttatggc ttcttgaaa acccttttg aagccttta ggacgcatt gttttaatt ggggcgaat aatttgatt cagaatgag caaaaattg tcgattttt acgctgttg aaggggtgg ttgactacg aaaaacgtt gatttggag gagttaatt acgcgaaat agggtcgct gcggggctt catttgggg acgctgatt gagaaggac ccaggaagg gtaagagac gccttgggg gtgattttc aaaatgatg gaagagaag aaactaaaa gtgaagatt gattcggtg catcccatg gagaagatt gttgatgcc acgaagttg ctggccgag aggaaaaac gtcgggaaa gactgtagg ttgggcgtc caattgtgc gtgagtttg aatcgcgcg ccttgcggc gccactgtc gcttccacg ggtaccgtt cgttttctc cgcgcatgt catttgtgt ttcttttgg aatcatttg ccatcttgg acacatctt gcacggttg gacataaga cacacgatg gctgacatc gacgacact catttcgaa actggggac tccggcgct tcggccact taccccatg caatgttcg gccctgcgc aagaacggc ttcgtcatg ctcaagtct cgaccttgc aagattgtc gaaatgtca acttccaaa actggaaaa cacggacac gccaaagtg catttggta ggcattgat atttttaca ggtaaaaaa tacgaagat atatgtccc tcaacacac aatatggat gtcccgcac gttaagcgc gaggactac cagttgact gatatctct gatgatggc tatttgtct ctcatgtct gacaatggt gacctccgt gaggatctc aaagttcca gagggggac gtgggggcc caactacgc gctgagtac gacgccggc aaggacatt ctgtgtaca gtgctcaaa tcttgcggc gaggaggtg gtaattgct gtgaaaaca aacactgcg cttgataaa taa
- >TC031278 RA
tgcgtgcaa ccaccgcca aaaatgaca attctccct cccagaatt cgccaagtg tcataatga aaattccgt cgaaaaact cgtcgaaat ttgtgaatt ggtcgcgta aatagtggt agaatagaa atttcttac gatttgatg gtaaagtca gcataggca atgcaagag tgttttttg aaaatcctc gcgaggttt cgtttgggc ccttccaat gggcaattg cctggcgtg cttcaagga gcccaacag tacggccct ggacaactc gcccaattg ccgtaaaga agaaatgcc cgaagcgca gctgtatcc ggctccgag tcacgtgcc agcctcctc ggccccaac cgagacgat cctgagcaa cggcaacca cctaacagc ccttccgga acccgccaa caaccgttt ctaccctcg caagcccca ttcgaagtc ggcgccggc gatgggtct caccgatag caaaccatc cgacgcgaa actaaacgc acttttcga ccagtacaa ggacaacgc ggaggacac catcctcgc agagggcat cgaacagtt gtgcagaga cctgcagat ctcgcccga cgattttcg gattttggt actggcgtg gaagctcaa cgccgagca gatgtgtcg gttcaccag gtccgagtt tgtcacggg gttgaaggc catgcgggc cgactcggt caaggggat ccagggccg gctgcccga gctcgtcgc ggaggtcgg ccaggacgt ggagcagtt taaggattt gtacaggtt taccttccg gttcggcct ggattctgc ggcggggca gcgaatcct gccgacgga catggcggt ggtgctgtg gcggctggt gttcacaat gcgggaacc cccgatcct cgcgcgctg gctgtcgtt cctcgagag tcaccagtc ggttcgggg gattccccg cgacacgtg gaacatgtt tttgaattt cgccgaagc cgtgggcga cgacttgag ttgttacga cgacaacga ggcgtggcc gagcctgtt cgacgactt cgtcgagcc aagcaaaca ctacttgtt gagccctac ccataaaat ggaaacgag cagagccat cgtggtgaa aaaattcgg cagttacga cgtccttag cgtggaaca ctttgcctt gcctccttt ggagggcaa tgtcgaagt gaaagtgga atatggggg cgtaaactt tgccgattt gtacacccg gcaaggttt gatgcacaa caaaaaact gccgtttgt tttggggat ggaatgtgt gggcacaat tgtgaaaat cggggccga aaacacaca actcaaagt gggccaaca tgtcatctg ttatgatta tcactgtgg gatgtaccg ggacgtagt cagggtaac ccccgacaa atgctaccc tctacccga ccacgtgac ttgggagga gggtgctgc gattttcgt caattactt aaccgccta cttttccat tatcgagct cgggaattt gcggcaaaa tgagacagt tttgatact ctcatgcgg ggggggtgt ggggtgggc gggcaccca gctggccaa aaccgtccc cggggtcac agtggtggg caccacagc ccccttcaa gcaccccga agtcaaagc caacggcgt cgatatcac cctaacttt ggacgagaa tttccaaaa ggaaataac aaaagagtg tccccaagg ctttgattt aattctgtc taattacgc cggcccgct ttatggctt cttgaaaac ccttttgaa gcctttagg acgcattgt tttaattgg ggcgaataa tttgattca gaatgagca aaaattgtc gatttttac gctgttgaa ggggtggtt gactacgaa aaacgttga tttggagga gttaattac gcgaaatag ggtcgctgc ggggcttca tttggggac gctgattga gaaggaccc aggaagggt aagagacgc cttgggggt gattttcaa aatgatgga agagaagaa actaaaagt gaagattga ttcggtgca tcccatgga gaagattgt tgatgccac gaagttgct ggccgagag gaaaaacgt cgggaaaga ctgtaggtt gggcgtcca attgtgcgt gagtttgaa tcgcgcgcc ttgcggcgc cactgtcgc ttccacggg taccgttcg ttttctccg cgcatgtca tttgtgttt cttttggaa tcatttgcc atcttggac acatcttgc acggttgga cataagaca cacgatggc tgacatcga cgacactca tttcgaaac tggggactc cggcgcttc ggccactta ccccatgca atgttcggc cctgcgcaa gaacggctt cgtcatgct caagtctcg accttgcaa gattgtcga aatgtcaac ttccaaaac tggaaaaca cggacacgc caaagtgca tttggtagg cattgatat ttttacagg taaaaaata cgaagatat atgtccctc aacacacaa tatggatgt cccgcacgt taagcgcga ggactacca gttgactga tatctctga tgatggcta tttgtctct catgtctga caatggtga cctccgtga ggatctcaa agttccaga gggggacgt gggggccca actacgcgc tgagtacga cgccggcaa ggacattct gtgtacagt gctcaaatc ttgcggcga ggaggtggt aattgctgt gaaaacaaa cactgcgct tgataaata ataccgatc acgtgacgt gggggccat agcctgttt tcaccaagt ccagaggcc tccactgcc gctaagcta tataattat cgtattgga ccagttata tcaacagct tacctacac caggtggac tcgcaggtc gtcaatgtg gtgtttaca ttttacatc tatttgtac ttttgttgg cgaaattca aatgcaaaa gctacaatt atgttcaat aaaaattta atttaattt taaatcaat gtaaatttt tattcaagc tccaatcag tcgagccac ctgtttctc atttttcac atcatcaga gtttttcta gtttcggga agcagctgg ctctgactc cgaataact tgttgatat tgcttttgt tcagtaagt atagaactt tttttaatt gttttgata ttgtttagg cgtaagttt taagtaact gaccaattg atttttgca ataattttc tggaattgg ttgaaatac aataacata ggaacc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
