Status
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Gene information
General information
TC031278
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006404,TC006406,TC006405 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8468958..8481925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031278
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04134

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031278 RA
      MGNCLACFK EPNSTALDN SPNCRKEEM PEAQLYPAP 
SHVPASSAP TETILSNGN HLTALPEPA NNRFYPRKP 
HSKSAPAMG LTDSKPSDA KLNALFDQY KDNAEDTIL 
AEGIEQLCR DLQISPDDF RILVLAWKL NAEQMCRFT 
RSEFVTGLK AMRADSVKG IQGRLPELV AEVGQDVEQ 
FKDLYRFTF RFGLDSAAG QRILPTDMA VVLWRLVFT 
MREPPILAR WLSFLESHQ SVRGIPRDT WNMFLNFAE 
AVGDDLSCY DDNEAWPSL FDDFVEPSK HYLLSPTHK 
METSRAIVV KKFGSYDVL SVEHFALPP LEGNVEVKV 
EYGGVNFAD LYTRQGLMH NKKLPFVLG MECVGTIVK 
IGAENTQLK VGQHVICYD YHCGMYRDV VRVTPDKCY 
PLPDHVTWE EGAAIFVNY LTAYFSIIE LGNLRQNET 
VLILSCGGG VGWAGTQLA KTVPGVTVV GTTAPFKHP 
EVKANGVDI TLTLDENFQ KEITKECPQ GFDLILSNY 
AGPLYGFLK TLLKPLGRI VLIGANNLI QNEQKLSIF 
TLLKGWLTT KNVDLEELI TRNRVAAGL HLGTLIEKD 
PGRVRDALG VIFKMMEEK KLKVKIDSV HPMEKIVDA 
TKLLAERKN VGKDCRLGV QLCVSLNRA PCGATVAST 
GTVRFLRAC HLCFFWNHL PSWTHLARL DIRHTMADI 
DDTHFETGD SGASATYPM QCSALRKNG FVMLKSRPC 
KIVEMSTSK TGKHGHAKV HLVGIDIFT GKKYEDICP 
STHNMDVPH VKREDYQLT DISDDGYLS LMSDNGDLR 
EDLKVPEGD VGAQLRAEY DAGKDILCT VLKSCGEEV 
VIAVKTNTA LDK
    CDS sequence
    • >TC031278 RA
      atgggcaat tgcctggcg tgcttcaag gagcccaac 
agtacggcc ctggacaac tcgcccaat tgccgtaaa 
gaagaaatg cccgaagcg cagctgtat ccggctccg 
agtcacgtg ccagcctcc tcggcccca accgagacg 
atcctgagc aacggcaac cacctaaca gcccttccg 
gaacccgcc aacaaccgt ttctaccct cgcaagccc 
cattcgaag tcggcgccg gcgatgggt ctcaccgat 
agcaaacca tccgacgcg aaactaaac gcacttttc 
gaccagtac aaggacaac gcggaggac accatcctc 
gcagagggc atcgaacag ttgtgcaga gacctgcag 
atctcgccc gacgatttt cggattttg gtactggcg 
tggaagctc aacgccgag cagatgtgt cggttcacc 
aggtccgag tttgtcacg gggttgaag gccatgcgg 
gccgactcg gtcaagggg atccagggc cggctgccc 
gagctcgtc gcggaggtc ggccaggac gtggagcag 
tttaaggat ttgtacagg tttaccttc cggttcggc 
ctggattct gcggcgggg cagcgaatc ctgccgacg 
gacatggcg gtggtgctg tggcggctg gtgttcaca 
atgcgggaa cccccgatc ctcgcgcgc tggctgtcg 
ttcctcgag agtcaccag tcggttcgg gggattccc 
cgcgacacg tggaacatg tttttgaat ttcgccgaa 
gccgtgggc gacgacttg agttgttac gacgacaac 
gaggcgtgg ccgagcctg ttcgacgac ttcgtcgag 
ccaagcaaa cactacttg ttgagccct acccataaa 
atggaaacg agcagagcc atcgtggtg aaaaaattc 
ggcagttac gacgtcctt agcgtggaa cactttgcc 
ttgcctcct ttggagggc aatgtcgaa gtgaaagtg 
gaatatggg ggcgtaaac tttgccgat ttgtacacc 
cggcaaggt ttgatgcac aacaaaaaa ctgccgttt 
gttttgggg atggaatgt gtgggcaca attgtgaaa 
atcggggcc gaaaacaca caactcaaa gtgggccaa 
catgtcatc tgttatgat tatcactgt gggatgtac 
cgggacgta gtcagggta acccccgac aaatgctac 
cctctaccc gaccacgtg acttgggag gagggtgct 
gcgattttc gtcaattac ttaaccgcc tacttttcc 
attatcgag ctcgggaat ttgcggcaa aatgagaca 
gttttgata ctctcatgc ggggggggt gtggggtgg 
gcgggcacc cagctggcc aaaaccgtc cccggggtc 
acagtggtg ggcaccaca gcccccttc aagcacccc 
gaagtcaaa gccaacggc gtcgatatc accctaact 
ttggacgag aatttccaa aaggaaata acaaaagag 
tgtccccaa ggctttgat ttaattctg tctaattac 
gccggcccg ctttatggc ttcttgaaa acccttttg 
aagccttta ggacgcatt gttttaatt ggggcgaat 
aatttgatt cagaatgag caaaaattg tcgattttt 
acgctgttg aaggggtgg ttgactacg aaaaacgtt 
gatttggag gagttaatt acgcgaaat agggtcgct 
gcggggctt catttgggg acgctgatt gagaaggac 
ccaggaagg gtaagagac gccttgggg gtgattttc 
aaaatgatg gaagagaag aaactaaaa gtgaagatt 
gattcggtg catcccatg gagaagatt gttgatgcc 
acgaagttg ctggccgag aggaaaaac gtcgggaaa 
gactgtagg ttgggcgtc caattgtgc gtgagtttg 
aatcgcgcg ccttgcggc gccactgtc gcttccacg 
ggtaccgtt cgttttctc cgcgcatgt catttgtgt 
ttcttttgg aatcatttg ccatcttgg acacatctt 
gcacggttg gacataaga cacacgatg gctgacatc 
gacgacact catttcgaa actggggac tccggcgct 
tcggccact taccccatg caatgttcg gccctgcgc 
aagaacggc ttcgtcatg ctcaagtct cgaccttgc 
aagattgtc gaaatgtca acttccaaa actggaaaa 
cacggacac gccaaagtg catttggta ggcattgat 
atttttaca ggtaaaaaa tacgaagat atatgtccc 
tcaacacac aatatggat gtcccgcac gttaagcgc 
gaggactac cagttgact gatatctct gatgatggc 
tatttgtct ctcatgtct gacaatggt gacctccgt 
gaggatctc aaagttcca gagggggac gtgggggcc 
caactacgc gctgagtac gacgccggc aaggacatt 
ctgtgtaca gtgctcaaa tcttgcggc gaggaggtg 
gtaattgct gtgaaaaca aacactgcg cttgataaa 
taa
    mRNA sequence
    • >TC031278 RA
      tgcgtgcaa ccaccgcca aaaatgaca attctccct 
cccagaatt cgccaagtg tcataatga aaattccgt 
cgaaaaact cgtcgaaat ttgtgaatt ggtcgcgta 
aatagtggt agaatagaa atttcttac gatttgatg 
gtaaagtca gcataggca atgcaagag tgttttttg 
aaaatcctc gcgaggttt cgtttgggc ccttccaat 
gggcaattg cctggcgtg cttcaagga gcccaacag 
tacggccct ggacaactc gcccaattg ccgtaaaga 
agaaatgcc cgaagcgca gctgtatcc ggctccgag 
tcacgtgcc agcctcctc ggccccaac cgagacgat 
cctgagcaa cggcaacca cctaacagc ccttccgga 
acccgccaa caaccgttt ctaccctcg caagcccca 
ttcgaagtc ggcgccggc gatgggtct caccgatag 
caaaccatc cgacgcgaa actaaacgc acttttcga 
ccagtacaa ggacaacgc ggaggacac catcctcgc 
agagggcat cgaacagtt gtgcagaga cctgcagat 
ctcgcccga cgattttcg gattttggt actggcgtg 
gaagctcaa cgccgagca gatgtgtcg gttcaccag 
gtccgagtt tgtcacggg gttgaaggc catgcgggc 
cgactcggt caaggggat ccagggccg gctgcccga 
gctcgtcgc ggaggtcgg ccaggacgt ggagcagtt 
taaggattt gtacaggtt taccttccg gttcggcct 
ggattctgc ggcggggca gcgaatcct gccgacgga 
catggcggt ggtgctgtg gcggctggt gttcacaat 
gcgggaacc cccgatcct cgcgcgctg gctgtcgtt 
cctcgagag tcaccagtc ggttcgggg gattccccg 
cgacacgtg gaacatgtt tttgaattt cgccgaagc 
cgtgggcga cgacttgag ttgttacga cgacaacga 
ggcgtggcc gagcctgtt cgacgactt cgtcgagcc 
aagcaaaca ctacttgtt gagccctac ccataaaat 
ggaaacgag cagagccat cgtggtgaa aaaattcgg 
cagttacga cgtccttag cgtggaaca ctttgcctt 
gcctccttt ggagggcaa tgtcgaagt gaaagtgga 
atatggggg cgtaaactt tgccgattt gtacacccg 
gcaaggttt gatgcacaa caaaaaact gccgtttgt 
tttggggat ggaatgtgt gggcacaat tgtgaaaat 
cggggccga aaacacaca actcaaagt gggccaaca 
tgtcatctg ttatgatta tcactgtgg gatgtaccg 
ggacgtagt cagggtaac ccccgacaa atgctaccc 
tctacccga ccacgtgac ttgggagga gggtgctgc 
gattttcgt caattactt aaccgccta cttttccat 
tatcgagct cgggaattt gcggcaaaa tgagacagt 
tttgatact ctcatgcgg ggggggtgt ggggtgggc 
gggcaccca gctggccaa aaccgtccc cggggtcac 
agtggtggg caccacagc ccccttcaa gcaccccga 
agtcaaagc caacggcgt cgatatcac cctaacttt 
ggacgagaa tttccaaaa ggaaataac aaaagagtg 
tccccaagg ctttgattt aattctgtc taattacgc 
cggcccgct ttatggctt cttgaaaac ccttttgaa 
gcctttagg acgcattgt tttaattgg ggcgaataa 
tttgattca gaatgagca aaaattgtc gatttttac 
gctgttgaa ggggtggtt gactacgaa aaacgttga 
tttggagga gttaattac gcgaaatag ggtcgctgc 
ggggcttca tttggggac gctgattga gaaggaccc 
aggaagggt aagagacgc cttgggggt gattttcaa 
aatgatgga agagaagaa actaaaagt gaagattga 
ttcggtgca tcccatgga gaagattgt tgatgccac 
gaagttgct ggccgagag gaaaaacgt cgggaaaga 
ctgtaggtt gggcgtcca attgtgcgt gagtttgaa 
tcgcgcgcc ttgcggcgc cactgtcgc ttccacggg 
taccgttcg ttttctccg cgcatgtca tttgtgttt 
cttttggaa tcatttgcc atcttggac acatcttgc 
acggttgga cataagaca cacgatggc tgacatcga 
cgacactca tttcgaaac tggggactc cggcgcttc 
ggccactta ccccatgca atgttcggc cctgcgcaa 
gaacggctt cgtcatgct caagtctcg accttgcaa 
gattgtcga aatgtcaac ttccaaaac tggaaaaca 
cggacacgc caaagtgca tttggtagg cattgatat 
ttttacagg taaaaaata cgaagatat atgtccctc 
aacacacaa tatggatgt cccgcacgt taagcgcga 
ggactacca gttgactga tatctctga tgatggcta 
tttgtctct catgtctga caatggtga cctccgtga 
ggatctcaa agttccaga gggggacgt gggggccca 
actacgcgc tgagtacga cgccggcaa ggacattct 
gtgtacagt gctcaaatc ttgcggcga ggaggtggt 
aattgctgt gaaaacaaa cactgcgct tgataaata 
ataccgatc acgtgacgt gggggccat agcctgttt 
tcaccaagt ccagaggcc tccactgcc gctaagcta 
tataattat cgtattgga ccagttata tcaacagct 
tacctacac caggtggac tcgcaggtc gtcaatgtg 
gtgtttaca ttttacatc tatttgtac ttttgttgg 
cgaaattca aatgcaaaa gctacaatt atgttcaat 
aaaaattta atttaattt taaatcaat gtaaatttt 
tattcaagc tccaatcag tcgagccac ctgtttctc 
atttttcac atcatcaga gtttttcta gtttcggga 
agcagctgg ctctgactc cgaataact tgttgatat 
tgcttttgt tcagtaagt atagaactt tttttaatt 
gttttgata ttgtttagg cgtaagttt taagtaact 
gaccaattg atttttgca ataattttc tggaattgg 
ttgaaatac aataacata ggaacc
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