Status
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Gene information
General information
TC031278
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006404,TC006406,TC006405
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8468958..8481925
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031278
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_04134

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031278 RA
      MGNCLACFK EPNSTALDN SPNCRKEEM PEAQLYPAP 
      SHVPASSAP TETILSNGN HLTALPEPA NNRFYPRKP 
      HSKSAPAMG LTDSKPSDA KLNALFDQY KDNAEDTIL 
      AEGIEQLCR DLQISPDDF RILVLAWKL NAEQMCRFT 
      RSEFVTGLK AMRADSVKG IQGRLPELV AEVGQDVEQ 
      FKDLYRFTF RFGLDSAAG QRILPTDMA VVLWRLVFT 
      MREPPILAR WLSFLESHQ SVRGIPRDT WNMFLNFAE 
      AVGDDLSCY DDNEAWPSL FDDFVEPSK HYLLSPTHK 
      METSRAIVV KKFGSYDVL SVEHFALPP LEGNVEVKV 
      EYGGVNFAD LYTRQGLMH NKKLPFVLG MECVGTIVK 
      IGAENTQLK VGQHVICYD YHCGMYRDV VRVTPDKCY 
      PLPDHVTWE EGAAIFVNY LTAYFSIIE LGNLRQNET 
      VLILSCGGG VGWAGTQLA KTVPGVTVV GTTAPFKHP 
      EVKANGVDI TLTLDENFQ KEITKECPQ GFDLILSNY 
      AGPLYGFLK TLLKPLGRI VLIGANNLI QNEQKLSIF 
      TLLKGWLTT KNVDLEELI TRNRVAAGL HLGTLIEKD 
      PGRVRDALG VIFKMMEEK KLKVKIDSV HPMEKIVDA 
      TKLLAERKN VGKDCRLGV QLCVSLNRA PCGATVAST 
      GTVRFLRAC HLCFFWNHL PSWTHLARL DIRHTMADI 
      DDTHFETGD SGASATYPM QCSALRKNG FVMLKSRPC 
      KIVEMSTSK TGKHGHAKV HLVGIDIFT GKKYEDICP 
      STHNMDVPH VKREDYQLT DISDDGYLS LMSDNGDLR 
      EDLKVPEGD VGAQLRAEY DAGKDILCT VLKSCGEEV 
      VIAVKTNTA LDK
    CDS sequence
    • >TC031278 RA
      atgggcaat tgcctggcg tgcttcaag gagcccaac 
      agtacggcc ctggacaac tcgcccaat tgccgtaaa 
      gaagaaatg cccgaagcg cagctgtat ccggctccg 
      agtcacgtg ccagcctcc tcggcccca accgagacg 
      atcctgagc aacggcaac cacctaaca gcccttccg 
      gaacccgcc aacaaccgt ttctaccct cgcaagccc 
      cattcgaag tcggcgccg gcgatgggt ctcaccgat 
      agcaaacca tccgacgcg aaactaaac gcacttttc 
      gaccagtac aaggacaac gcggaggac accatcctc 
      gcagagggc atcgaacag ttgtgcaga gacctgcag 
      atctcgccc gacgatttt cggattttg gtactggcg 
      tggaagctc aacgccgag cagatgtgt cggttcacc 
      aggtccgag tttgtcacg gggttgaag gccatgcgg 
      gccgactcg gtcaagggg atccagggc cggctgccc 
      gagctcgtc gcggaggtc ggccaggac gtggagcag 
      tttaaggat ttgtacagg tttaccttc cggttcggc 
      ctggattct gcggcgggg cagcgaatc ctgccgacg 
      gacatggcg gtggtgctg tggcggctg gtgttcaca 
      atgcgggaa cccccgatc ctcgcgcgc tggctgtcg 
      ttcctcgag agtcaccag tcggttcgg gggattccc 
      cgcgacacg tggaacatg tttttgaat ttcgccgaa 
      gccgtgggc gacgacttg agttgttac gacgacaac 
      gaggcgtgg ccgagcctg ttcgacgac ttcgtcgag 
      ccaagcaaa cactacttg ttgagccct acccataaa 
      atggaaacg agcagagcc atcgtggtg aaaaaattc 
      ggcagttac gacgtcctt agcgtggaa cactttgcc 
      ttgcctcct ttggagggc aatgtcgaa gtgaaagtg 
      gaatatggg ggcgtaaac tttgccgat ttgtacacc 
      cggcaaggt ttgatgcac aacaaaaaa ctgccgttt 
      gttttgggg atggaatgt gtgggcaca attgtgaaa 
      atcggggcc gaaaacaca caactcaaa gtgggccaa 
      catgtcatc tgttatgat tatcactgt gggatgtac 
      cgggacgta gtcagggta acccccgac aaatgctac 
      cctctaccc gaccacgtg acttgggag gagggtgct 
      gcgattttc gtcaattac ttaaccgcc tacttttcc 
      attatcgag ctcgggaat ttgcggcaa aatgagaca 
      gttttgata ctctcatgc ggggggggt gtggggtgg 
      gcgggcacc cagctggcc aaaaccgtc cccggggtc 
      acagtggtg ggcaccaca gcccccttc aagcacccc 
      gaagtcaaa gccaacggc gtcgatatc accctaact 
      ttggacgag aatttccaa aaggaaata acaaaagag 
      tgtccccaa ggctttgat ttaattctg tctaattac 
      gccggcccg ctttatggc ttcttgaaa acccttttg 
      aagccttta ggacgcatt gttttaatt ggggcgaat 
      aatttgatt cagaatgag caaaaattg tcgattttt 
      acgctgttg aaggggtgg ttgactacg aaaaacgtt 
      gatttggag gagttaatt acgcgaaat agggtcgct 
      gcggggctt catttgggg acgctgatt gagaaggac 
      ccaggaagg gtaagagac gccttgggg gtgattttc 
      aaaatgatg gaagagaag aaactaaaa gtgaagatt 
      gattcggtg catcccatg gagaagatt gttgatgcc 
      acgaagttg ctggccgag aggaaaaac gtcgggaaa 
      gactgtagg ttgggcgtc caattgtgc gtgagtttg 
      aatcgcgcg ccttgcggc gccactgtc gcttccacg 
      ggtaccgtt cgttttctc cgcgcatgt catttgtgt 
      ttcttttgg aatcatttg ccatcttgg acacatctt 
      gcacggttg gacataaga cacacgatg gctgacatc 
      gacgacact catttcgaa actggggac tccggcgct 
      tcggccact taccccatg caatgttcg gccctgcgc 
      aagaacggc ttcgtcatg ctcaagtct cgaccttgc 
      aagattgtc gaaatgtca acttccaaa actggaaaa 
      cacggacac gccaaagtg catttggta ggcattgat 
      atttttaca ggtaaaaaa tacgaagat atatgtccc 
      tcaacacac aatatggat gtcccgcac gttaagcgc 
      gaggactac cagttgact gatatctct gatgatggc 
      tatttgtct ctcatgtct gacaatggt gacctccgt 
      gaggatctc aaagttcca gagggggac gtgggggcc 
      caactacgc gctgagtac gacgccggc aaggacatt 
      ctgtgtaca gtgctcaaa tcttgcggc gaggaggtg 
      gtaattgct gtgaaaaca aacactgcg cttgataaa 
      taa
    mRNA sequence
    • >TC031278 RA
      tgcgtgcaa ccaccgcca aaaatgaca attctccct 
      cccagaatt cgccaagtg tcataatga aaattccgt 
      cgaaaaact cgtcgaaat ttgtgaatt ggtcgcgta 
      aatagtggt agaatagaa atttcttac gatttgatg 
      gtaaagtca gcataggca atgcaagag tgttttttg 
      aaaatcctc gcgaggttt cgtttgggc ccttccaat 
      gggcaattg cctggcgtg cttcaagga gcccaacag 
      tacggccct ggacaactc gcccaattg ccgtaaaga 
      agaaatgcc cgaagcgca gctgtatcc ggctccgag 
      tcacgtgcc agcctcctc ggccccaac cgagacgat 
      cctgagcaa cggcaacca cctaacagc ccttccgga 
      acccgccaa caaccgttt ctaccctcg caagcccca 
      ttcgaagtc ggcgccggc gatgggtct caccgatag 
      caaaccatc cgacgcgaa actaaacgc acttttcga 
      ccagtacaa ggacaacgc ggaggacac catcctcgc 
      agagggcat cgaacagtt gtgcagaga cctgcagat 
      ctcgcccga cgattttcg gattttggt actggcgtg 
      gaagctcaa cgccgagca gatgtgtcg gttcaccag 
      gtccgagtt tgtcacggg gttgaaggc catgcgggc 
      cgactcggt caaggggat ccagggccg gctgcccga 
      gctcgtcgc ggaggtcgg ccaggacgt ggagcagtt 
      taaggattt gtacaggtt taccttccg gttcggcct 
      ggattctgc ggcggggca gcgaatcct gccgacgga 
      catggcggt ggtgctgtg gcggctggt gttcacaat 
      gcgggaacc cccgatcct cgcgcgctg gctgtcgtt 
      cctcgagag tcaccagtc ggttcgggg gattccccg 
      cgacacgtg gaacatgtt tttgaattt cgccgaagc 
      cgtgggcga cgacttgag ttgttacga cgacaacga 
      ggcgtggcc gagcctgtt cgacgactt cgtcgagcc 
      aagcaaaca ctacttgtt gagccctac ccataaaat 
      ggaaacgag cagagccat cgtggtgaa aaaattcgg 
      cagttacga cgtccttag cgtggaaca ctttgcctt 
      gcctccttt ggagggcaa tgtcgaagt gaaagtgga 
      atatggggg cgtaaactt tgccgattt gtacacccg 
      gcaaggttt gatgcacaa caaaaaact gccgtttgt 
      tttggggat ggaatgtgt gggcacaat tgtgaaaat 
      cggggccga aaacacaca actcaaagt gggccaaca 
      tgtcatctg ttatgatta tcactgtgg gatgtaccg 
      ggacgtagt cagggtaac ccccgacaa atgctaccc 
      tctacccga ccacgtgac ttgggagga gggtgctgc 
      gattttcgt caattactt aaccgccta cttttccat 
      tatcgagct cgggaattt gcggcaaaa tgagacagt 
      tttgatact ctcatgcgg ggggggtgt ggggtgggc 
      gggcaccca gctggccaa aaccgtccc cggggtcac 
      agtggtggg caccacagc ccccttcaa gcaccccga 
      agtcaaagc caacggcgt cgatatcac cctaacttt 
      ggacgagaa tttccaaaa ggaaataac aaaagagtg 
      tccccaagg ctttgattt aattctgtc taattacgc 
      cggcccgct ttatggctt cttgaaaac ccttttgaa 
      gcctttagg acgcattgt tttaattgg ggcgaataa 
      tttgattca gaatgagca aaaattgtc gatttttac 
      gctgttgaa ggggtggtt gactacgaa aaacgttga 
      tttggagga gttaattac gcgaaatag ggtcgctgc 
      ggggcttca tttggggac gctgattga gaaggaccc 
      aggaagggt aagagacgc cttgggggt gattttcaa 
      aatgatgga agagaagaa actaaaagt gaagattga 
      ttcggtgca tcccatgga gaagattgt tgatgccac 
      gaagttgct ggccgagag gaaaaacgt cgggaaaga 
      ctgtaggtt gggcgtcca attgtgcgt gagtttgaa 
      tcgcgcgcc ttgcggcgc cactgtcgc ttccacggg 
      taccgttcg ttttctccg cgcatgtca tttgtgttt 
      cttttggaa tcatttgcc atcttggac acatcttgc 
      acggttgga cataagaca cacgatggc tgacatcga 
      cgacactca tttcgaaac tggggactc cggcgcttc 
      ggccactta ccccatgca atgttcggc cctgcgcaa 
      gaacggctt cgtcatgct caagtctcg accttgcaa 
      gattgtcga aatgtcaac ttccaaaac tggaaaaca 
      cggacacgc caaagtgca tttggtagg cattgatat 
      ttttacagg taaaaaata cgaagatat atgtccctc 
      aacacacaa tatggatgt cccgcacgt taagcgcga 
      ggactacca gttgactga tatctctga tgatggcta 
      tttgtctct catgtctga caatggtga cctccgtga 
      ggatctcaa agttccaga gggggacgt gggggccca 
      actacgcgc tgagtacga cgccggcaa ggacattct 
      gtgtacagt gctcaaatc ttgcggcga ggaggtggt 
      aattgctgt gaaaacaaa cactgcgct tgataaata 
      ataccgatc acgtgacgt gggggccat agcctgttt 
      tcaccaagt ccagaggcc tccactgcc gctaagcta 
      tataattat cgtattgga ccagttata tcaacagct 
      tacctacac caggtggac tcgcaggtc gtcaatgtg 
      gtgtttaca ttttacatc tatttgtac ttttgttgg 
      cgaaattca aatgcaaaa gctacaatt atgttcaat 
      aaaaattta atttaattt taaatcaat gtaaatttt 
      tattcaagc tccaatcag tcgagccac ctgtttctc 
      atttttcac atcatcaga gtttttcta gtttcggga 
      agcagctgg ctctgactc cgaataact tgttgatat 
      tgcttttgt tcagtaagt atagaactt tttttaatt 
      gttttgata ttgtttagg cgtaagttt taagtaact 
      gaccaattg atttttgca ataattttc tggaattgg 
      ttgaaatac aataacata ggaacc
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