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Gene information
General information
TC006403 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8463659..8468622
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006403
    iBeetle screen iB_04132

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006403 RA
      MDTEFYNLH KALSAYKTS ISTSMDKID DIFSQAAST 
      FINPDCARG PNPKKYTIS LEIVTVLQH YIRILSTVE 
      SLADGLATK LSNLDRDAE PERETACNP FTSALLSDS 
      KECAVSCFF HNLKAQIAE RDVSSGVDS DNVSSDAET 
      IVDLKTENL DEYARLNLF DVNVKPENA PESEISNSQ 
      KATNNSKTQ IKFEVEADD SSGAETLVE QPVQGKNDL 
      LDHSRLNPF KADSNEPKT GAKKKKTVR IKEEPPCEN 
      AEIEYTALS KLLSMSVHD RRREAQKKF ENKSIKRDF 
      NWRNVTSPP RDKKVREKF SIYDILEDQ LSVGQKCVF 
      SHIISPAEF YLRTNQALF EMLQNSLNE DMNSQHQWV 
      VNKYKTKED ARTHMGNFC FAFLPNKRK WYRVEVLDW 
      LMEEELENI CIQLIDFGE TYYCKFEIL LPMTLFYSM 
      FPRMAVRCH FAAIYSPDP SKWPESSIE ALEQMSEME 
      AKTEFETIF VCKEGNSYG IDLLNEEKF GKKTLGQIL 
      VDSELALQV DISDGLEAL EDEDFEEYE NINEAITGY 
      DAKDEARLC PFTNAAGGC FKGARCKLE HGVLAKDGV 
      TTDKVLTMN QAFTVTPLP PKGQCVDVL VTGYISTCR 
      FYAQIVTAP SKTSNFNAN FQRLRESMN SNNIVHTYE 
      PFKLYPGLG EVVLIKENS EWFRGICVG LAENDKIEV 
      FFVDFGYKR EKKLKDLRQ MKEDLLYVP FQAVECMLE 
      GCQEKKDAP KNASQTFFD SCMNLKVFK ALVVESEEP 
      LRLSMWDSE GMEMGYVLK TCGLGEDRK KDVFPTKDC 
      YLSCN
    CDS sequence
    • >TC006403 RA
      atggatacg gaattttac aatttacac aaagccctt 
      tctgcctac aagacgtcc atcagcaca agtatggac 
      aaaattgac gatatcttt tcgcaagct gcaagtact 
      tttatcaat cccgattgt gccagaggt ccaaacccg 
      aaaaagtac acaatttcg ctcgaaatc gtaacagtt 
      ttgcagcat tatattagg attcttagc actgttgag 
      agcttagcc gacggtttg gcgaccaag ctctctaac 
      ctagaccgt gacgcagag cctgaaaga gaaacagct 
      tgcaatcct ttcacttcg gccctttta agcgactcg 
      aaagagtgt gcagtttcg tgcttcttc cataacttg 
      aaggcacaa attgccgaa agagatgtc agttcgggc 
      gtcgattct gacaatgtt agttcagac gcggaaacg 
      atagtggac ctaaaaacc gaaaatttg gacgaatac 
      gcacgattg aatctgttt gatgtcaac gttaagcct 
      gaaaatgcg cccgaaagt gaaatttcc aactctcaa 
      aaagccaca aacaattcg aaaactcaa atcaaattc 
      gaggttgaa gctgacgac agttcgggg gccgaaacg 
      cttgtggaa caaccagtt cagggaaaa aacgatttg 
      ttagatcac agtagactt aaccctttc aaagctgac 
      tcgaatgag cccaaaaca ggggcaaaa aagaagaaa 
      actgtaaga atcaaggag gagccgcct tgcgaaaac 
      gccgaaatc gaatatacg gccctgagc aaacttttg 
      tcgatgtct gtgcatgac aggcgtcgc gaggcgcag 
      aaaaagttt gaaaacaaa tccatcaaa cgcgatttc 
      aattggcgc aacgtcacc tcgccacca cgtgacaaa 
      aaagtgcga gaaaaattc tcgatttac gatattctt 
      gaggaccag ttaagtgtg ggccagaag tgtgttttc 
      agtcacata atctcgccc gccgagttt tatcttcgc 
      acgaatcag gcgttgttt gaaatgctg caaaatagc 
      ttgaacgag gatatgaat agccaacac cagtgggtt 
      gttaataaa tataaaacg aaggaggat gcaaggaca 
      catatgggc aatttttgt ttcgcgttt ttgcccaat 
      aagcggaag tggtacaga gtcgaggtg cttgattgg 
      ctgatggag gaagaattg gagaatatc tgcatccag 
      ttgattgat tttggcgaa acatactat tgcaaattt 
      gagatactc ctccccatg accctattt tactcaatg 
      tttccgcgt atggccgtc aggtgccac tttgcagcg 
      atttattca cctgatcct agtaaatgg ccagaatcg 
      agtatcgaa gccttggaa caaatgagc gaaatggag 
      gctaaaacc gaattcgag acgattttt gtatgcaag 
      gagggaaat tcgtatgga attgatctc ttgaatgag 
      gagaaattt gggaagaaa acgttgggt cagattttg 
      gtcgactcc gaacttgcc ctccaagtg gacatttca 
      gacggactt gaagcatta gaggatgaa gatttcgaa 
      gagtatgaa aacatcaac gaggccata actggatac 
      gatgccaaa gatgaagca agactttgc cccttcact 
      aatgcggca gggggctgt tttaaaggg gcaaggtgt 
      aagctagaa cacggcgtt ttggcgaaa gatggcgtc 
      acgacggat aaagttttg acgatgaat caagcgttc 
      actgtgacg cctttgccc cccaaaggc cagtgtgtc 
      gacgttttg gtaacgggg tacataagc acctgccgt 
      ttttacgca caaattgtc acagcaccg tccaagact 
      tcgaatttt aatgcaaac ttccagagg ctgagagag 
      agcatgaac tcgaataat atagtccac acttacgag 
      ccatttaaa ttgtatccc ggcctcggg gaggttgtt 
      ttgatcaaa gaaaactcg gaatggttt cgggggatt 
      tgcgttggc ttagccgaa aacgacaag attgaggtt 
      ttctttgtg gattttggt tacaaaaga gagaaaaaa 
      ctgaaagat cttagacaa atgaaagaa gatcttctt 
      tatgtgcca ttccaagcg gttgaatgt atgttggag 
      ggctgtcag gagaagaaa gatgcccca aaaaatgcc 
      tcccaaaca tttttcgac tcgtgcatg aatttaaaa 
      gtttttaag gctctagtt gttgaatca gaggaaccc 
      ctgagactg tctatgtgg gactctgaa ggcatggag 
      atgggctac gttctgaaa acgtgcggg ttaggagag 
      gaccgcaag aaagatgtt ttcccaacc aaagactgt 
      tatttaagt tgcaattga
    mRNA sequence
    • >TC006403 RA
      cggactcta acatggtga catctgaca tggacgttt 
      atttgacag tgacagtca cgcttttgt aactttttt 
      tgaaaactt ttttgtttg atgcatgat ggatacgga 
      attttacaa tttacacaa agccctttc tgcctacaa 
      gacgtccat cagcacaag tatggacaa aattgacga 
      tatcttttc gcaagctgc aagtacttt tatcaatcc 
      cgattgtgc cagaggtcc aaacccgaa aaagtacac 
      aatttcgct cgaaatcgt aacagtttt gcagcatta 
      tattaggat tcttagcac tgttgagag cttagccga 
      cggtttggc gaccaagct ctctaacct agaccgtga 
      cgcagagcc tgaaagaga aacagcttg caatccttt 
      cacttcggc ccttttaag cgactcgaa agagtgtgc 
      agtttcgtg cttcttcca taacttgaa ggcacaaat 
      tgccgaaag agatgtcag ttcgggcgt cgattctga 
      caatgttag ttcagacgc ggaaacgat agtggacct 
      aaaaaccga aaatttgga cgaatacgc acgattgaa 
      tctgtttga tgtcaacgt taagcctga aaatgcgcc 
      cgaaagtga aatttccaa ctctcaaaa agccacaaa 
      caattcgaa aactcaaat caaattcga ggttgaagc 
      tgacgacag ttcgggggc cgaaacgct tgtggaaca 
      accagttca gggaaaaaa cgatttgtt agatcacag 
      tagacttaa ccctttcaa agctgactc gaatgagcc 
      caaaacagg ggcaaaaaa gaagaaaac tgtaagaat 
      caaggagga gccgccttg cgaaaacgc cgaaatcga 
      atatacggc cctgagcaa acttttgtc gatgtctgt 
      gcatgacag gcgtcgcga ggcgcagaa aaagtttga 
      aaacaaatc catcaaacg cgatttcaa ttggcgcaa 
      cgtcacctc gccaccacg tgacaaaaa agtgcgaga 
      aaaattctc gatttacga tattcttga ggaccagtt 
      aagtgtggg ccagaagtg tgttttcag tcacataat 
      ctcgcccgc cgagtttta tcttcgcac gaatcaggc 
      gttgtttga aatgctgca aaatagctt gaacgagga 
      tatgaatag ccaacacca gtgggttgt taataaata 
      taaaacgaa ggaggatgc aaggacaca tatgggcaa 
      tttttgttt cgcgttttt gcccaataa gcggaagtg 
      gtacagagt cgaggtgct tgattggct gatggagga 
      agaattgga gaatatctg catccagtt gattgattt 
      tggcgaaac atactattg caaatttga gatactcct 
      ccccatgac cctatttta ctcaatgtt tccgcgtat 
      ggccgtcag gtgccactt tgcagcgat ttattcacc 
      tgatcctag taaatggcc agaatcgag tatcgaagc 
      cttggaaca aatgagcga aatggaggc taaaaccga 
      attcgagac gatttttgt atgcaagga gggaaattc 
      gtatggaat tgatctctt gaatgagga gaaatttgg 
      gaagaaaac gttgggtca gattttggt cgactccga 
      acttgccct ccaagtgga catttcaga cggacttga 
      agcattaga ggatgaaga tttcgaaga gtatgaaaa 
      catcaacga ggccataac tggatacga tgccaaaga 
      tgaagcaag actttgccc cttcactaa tgcggcagg 
      gggctgttt taaaggggc aaggtgtaa gctagaaca 
      cggcgtttt ggcgaaaga tggcgtcac gacggataa 
      agttttgac gatgaatca agcgttcac tgtgacgcc 
      tttgccccc caaaggcca gtgtgtcga cgttttggt 
      aacggggta cataagcac ctgccgttt ttacgcaca 
      aattgtcac agcaccgtc caagacttc gaattttaa 
      tgcaaactt ccagaggct gagagagag catgaactc 
      gaataatat agtccacac ttacgagcc atttaaatt 
      gtatcccgg cctcgggga ggttgtttt gatcaaaga 
      aaactcgga atggtttcg ggggatttg cgttggctt 
      agccgaaaa cgacaagat tgaggtttt ctttgtgga 
      ttttggtta caaaagaga gaaaaaact gaaagatct 
      tagacaaat gaaagaaga tcttcttta tgtgccatt 
      ccaagcggt tgaatgtat gttggaggg ctgtcagga 
      gaagaaaga tgccccaaa aaatgcctc ccaaacatt 
      tttcgactc gtgcatgaa tttaaaagt ttttaaggc 
      tctagttgt tgaatcaga ggaacccct gagactgtc 
      tatgtggga ctctgaagg catggagat gggctacgt 
      tctgaaaac gtgcgggtt aggagagga ccgcaagaa 
      agatgtttt cccaaccaa agactgtta tttaagttg 
      caattgatt tttccatga ctgtttaga gtgtatttt 
      aatttattt tattaaaga caattcaat ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY