General information | |||
TC006403 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 8463659..8468622 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC006403 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04132 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
previtellogenic egg chamber orientation irregular - Number of animals showing the phenotype: 2
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC006403 RA
MDTEFYNLH KALSAYKTS ISTSMDKID DIFSQAAST FINPDCARG PNPKKYTIS LEIVTVLQH YIRILSTVE SLADGLATK LSNLDRDAE PERETACNP FTSALLSDS KECAVSCFF HNLKAQIAE RDVSSGVDS DNVSSDAET IVDLKTENL DEYARLNLF DVNVKPENA PESEISNSQ KATNNSKTQ IKFEVEADD SSGAETLVE QPVQGKNDL LDHSRLNPF KADSNEPKT GAKKKKTVR IKEEPPCEN AEIEYTALS KLLSMSVHD RRREAQKKF ENKSIKRDF NWRNVTSPP RDKKVREKF SIYDILEDQ LSVGQKCVF SHIISPAEF YLRTNQALF EMLQNSLNE DMNSQHQWV VNKYKTKED ARTHMGNFC FAFLPNKRK WYRVEVLDW LMEEELENI CIQLIDFGE TYYCKFEIL LPMTLFYSM FPRMAVRCH FAAIYSPDP SKWPESSIE ALEQMSEME AKTEFETIF VCKEGNSYG IDLLNEEKF GKKTLGQIL VDSELALQV DISDGLEAL EDEDFEEYE NINEAITGY DAKDEARLC PFTNAAGGC FKGARCKLE HGVLAKDGV TTDKVLTMN QAFTVTPLP PKGQCVDVL VTGYISTCR FYAQIVTAP SKTSNFNAN FQRLRESMN SNNIVHTYE PFKLYPGLG EVVLIKENS EWFRGICVG LAENDKIEV FFVDFGYKR EKKLKDLRQ MKEDLLYVP FQAVECMLE GCQEKKDAP KNASQTFFD SCMNLKVFK ALVVESEEP LRLSMWDSE GMEMGYVLK TCGLGEDRK KDVFPTKDC YLSCN
- >TC006403 RA
atggatacg gaattttac aatttacac aaagccctt tctgcctac aagacgtcc atcagcaca agtatggac aaaattgac gatatcttt tcgcaagct gcaagtact tttatcaat cccgattgt gccagaggt ccaaacccg aaaaagtac acaatttcg ctcgaaatc gtaacagtt ttgcagcat tatattagg attcttagc actgttgag agcttagcc gacggtttg gcgaccaag ctctctaac ctagaccgt gacgcagag cctgaaaga gaaacagct tgcaatcct ttcacttcg gccctttta agcgactcg aaagagtgt gcagtttcg tgcttcttc cataacttg aaggcacaa attgccgaa agagatgtc agttcgggc gtcgattct gacaatgtt agttcagac gcggaaacg atagtggac ctaaaaacc gaaaatttg gacgaatac gcacgattg aatctgttt gatgtcaac gttaagcct gaaaatgcg cccgaaagt gaaatttcc aactctcaa aaagccaca aacaattcg aaaactcaa atcaaattc gaggttgaa gctgacgac agttcgggg gccgaaacg cttgtggaa caaccagtt cagggaaaa aacgatttg ttagatcac agtagactt aaccctttc aaagctgac tcgaatgag cccaaaaca ggggcaaaa aagaagaaa actgtaaga atcaaggag gagccgcct tgcgaaaac gccgaaatc gaatatacg gccctgagc aaacttttg tcgatgtct gtgcatgac aggcgtcgc gaggcgcag aaaaagttt gaaaacaaa tccatcaaa cgcgatttc aattggcgc aacgtcacc tcgccacca cgtgacaaa aaagtgcga gaaaaattc tcgatttac gatattctt gaggaccag ttaagtgtg ggccagaag tgtgttttc agtcacata atctcgccc gccgagttt tatcttcgc acgaatcag gcgttgttt gaaatgctg caaaatagc ttgaacgag gatatgaat agccaacac cagtgggtt gttaataaa tataaaacg aaggaggat gcaaggaca catatgggc aatttttgt ttcgcgttt ttgcccaat aagcggaag tggtacaga gtcgaggtg cttgattgg ctgatggag gaagaattg gagaatatc tgcatccag ttgattgat tttggcgaa acatactat tgcaaattt gagatactc ctccccatg accctattt tactcaatg tttccgcgt atggccgtc aggtgccac tttgcagcg atttattca cctgatcct agtaaatgg ccagaatcg agtatcgaa gccttggaa caaatgagc gaaatggag gctaaaacc gaattcgag acgattttt gtatgcaag gagggaaat tcgtatgga attgatctc ttgaatgag gagaaattt gggaagaaa acgttgggt cagattttg gtcgactcc gaacttgcc ctccaagtg gacatttca gacggactt gaagcatta gaggatgaa gatttcgaa gagtatgaa aacatcaac gaggccata actggatac gatgccaaa gatgaagca agactttgc cccttcact aatgcggca gggggctgt tttaaaggg gcaaggtgt aagctagaa cacggcgtt ttggcgaaa gatggcgtc acgacggat aaagttttg acgatgaat caagcgttc actgtgacg cctttgccc cccaaaggc cagtgtgtc gacgttttg gtaacgggg tacataagc acctgccgt ttttacgca caaattgtc acagcaccg tccaagact tcgaatttt aatgcaaac ttccagagg ctgagagag agcatgaac tcgaataat atagtccac acttacgag ccatttaaa ttgtatccc ggcctcggg gaggttgtt ttgatcaaa gaaaactcg gaatggttt cgggggatt tgcgttggc ttagccgaa aacgacaag attgaggtt ttctttgtg gattttggt tacaaaaga gagaaaaaa ctgaaagat cttagacaa atgaaagaa gatcttctt tatgtgcca ttccaagcg gttgaatgt atgttggag ggctgtcag gagaagaaa gatgcccca aaaaatgcc tcccaaaca tttttcgac tcgtgcatg aatttaaaa gtttttaag gctctagtt gttgaatca gaggaaccc ctgagactg tctatgtgg gactctgaa ggcatggag atgggctac gttctgaaa acgtgcggg ttaggagag gaccgcaag aaagatgtt ttcccaacc aaagactgt tatttaagt tgcaattga
- >TC006403 RA
cggactcta acatggtga catctgaca tggacgttt atttgacag tgacagtca cgcttttgt aactttttt tgaaaactt ttttgtttg atgcatgat ggatacgga attttacaa tttacacaa agccctttc tgcctacaa gacgtccat cagcacaag tatggacaa aattgacga tatcttttc gcaagctgc aagtacttt tatcaatcc cgattgtgc cagaggtcc aaacccgaa aaagtacac aatttcgct cgaaatcgt aacagtttt gcagcatta tattaggat tcttagcac tgttgagag cttagccga cggtttggc gaccaagct ctctaacct agaccgtga cgcagagcc tgaaagaga aacagcttg caatccttt cacttcggc ccttttaag cgactcgaa agagtgtgc agtttcgtg cttcttcca taacttgaa ggcacaaat tgccgaaag agatgtcag ttcgggcgt cgattctga caatgttag ttcagacgc ggaaacgat agtggacct aaaaaccga aaatttgga cgaatacgc acgattgaa tctgtttga tgtcaacgt taagcctga aaatgcgcc cgaaagtga aatttccaa ctctcaaaa agccacaaa caattcgaa aactcaaat caaattcga ggttgaagc tgacgacag ttcgggggc cgaaacgct tgtggaaca accagttca gggaaaaaa cgatttgtt agatcacag tagacttaa ccctttcaa agctgactc gaatgagcc caaaacagg ggcaaaaaa gaagaaaac tgtaagaat caaggagga gccgccttg cgaaaacgc cgaaatcga atatacggc cctgagcaa acttttgtc gatgtctgt gcatgacag gcgtcgcga ggcgcagaa aaagtttga aaacaaatc catcaaacg cgatttcaa ttggcgcaa cgtcacctc gccaccacg tgacaaaaa agtgcgaga aaaattctc gatttacga tattcttga ggaccagtt aagtgtggg ccagaagtg tgttttcag tcacataat ctcgcccgc cgagtttta tcttcgcac gaatcaggc gttgtttga aatgctgca aaatagctt gaacgagga tatgaatag ccaacacca gtgggttgt taataaata taaaacgaa ggaggatgc aaggacaca tatgggcaa tttttgttt cgcgttttt gcccaataa gcggaagtg gtacagagt cgaggtgct tgattggct gatggagga agaattgga gaatatctg catccagtt gattgattt tggcgaaac atactattg caaatttga gatactcct ccccatgac cctatttta ctcaatgtt tccgcgtat ggccgtcag gtgccactt tgcagcgat ttattcacc tgatcctag taaatggcc agaatcgag tatcgaagc cttggaaca aatgagcga aatggaggc taaaaccga attcgagac gatttttgt atgcaagga gggaaattc gtatggaat tgatctctt gaatgagga gaaatttgg gaagaaaac gttgggtca gattttggt cgactccga acttgccct ccaagtgga catttcaga cggacttga agcattaga ggatgaaga tttcgaaga gtatgaaaa catcaacga ggccataac tggatacga tgccaaaga tgaagcaag actttgccc cttcactaa tgcggcagg gggctgttt taaaggggc aaggtgtaa gctagaaca cggcgtttt ggcgaaaga tggcgtcac gacggataa agttttgac gatgaatca agcgttcac tgtgacgcc tttgccccc caaaggcca gtgtgtcga cgttttggt aacggggta cataagcac ctgccgttt ttacgcaca aattgtcac agcaccgtc caagacttc gaattttaa tgcaaactt ccagaggct gagagagag catgaactc gaataatat agtccacac ttacgagcc atttaaatt gtatcccgg cctcgggga ggttgtttt gatcaaaga aaactcgga atggtttcg ggggatttg cgttggctt agccgaaaa cgacaagat tgaggtttt ctttgtgga ttttggtta caaaagaga gaaaaaact gaaagatct tagacaaat gaaagaaga tcttcttta tgtgccatt ccaagcggt tgaatgtat gttggaggg ctgtcagga gaagaaaga tgccccaaa aaatgcctc ccaaacatt tttcgactc gtgcatgaa tttaaaagt ttttaaggc tctagttgt tgaatcaga ggaacccct gagactgtc tatgtggga ctctgaagg catggagat gggctacgt tctgaaaac gtgcgggtt aggagagga ccgcaagaa agatgtttt cccaaccaa agactgtta tttaagttg caattgatt tttccatga ctgtttaga gtgtatttt aatttattt tattaaaga caattcaat ttt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
