Status
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Gene information
General information
TC034063
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006395,TC006396
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8395906..8400710
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC034063
      iBeetle screen iB_04131

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC034063 RA
        MKMKTVLLV FGLFDLGSC IRCYVCTSE ASSNQYEYD 
        ACLYGDERY LQAVACPEP SVCTAYHYQ TLIPGSLNQ 
        LSTSRGCQA LRYGATCED IFNDLRRTG SVLPGQHIC 
        ATCSEDLCN TAMGLSPGL CLMLVVIFL LMALRVICA 
        ILGVFLVNN LDSVTTLQC YSCTASLGS NFRCENLSY 
        SGGSLPYYT CMYGDAVCA KYIIEYGDS LMIHRSCQH 
        RDICAVLAS KYNNYYSKL LDCQTCSDH DWCNGANIS 
        DFKMSLKIC ILVTLLLAH YGDSLKCYT CETQDLNSP 
        CEDLSRGKD KIKTHDCSE GEVCAVYKT KNPSTGIIT 
        VKRSCEPKD ICEELYEEN SDFKKLEKC KICKTDLCD 
        SGTTLSISA ITFILALMI GAFIN
      CDS sequence
      • >TC034063 RA
        atgaagatg aagacggtt ttgctcgtt tttggactt 
        ttcgatctg ggctcctgc atccgctgc tacgtgtgc 
        acttcggag gcctccagc aaccaatac gaatacgac 
        gcgtgtctc tacggcgac gagcgctac ctccaggcc 
        gttgcgtgc cccgagccc tccgtatgc accgcctac 
        cactaccaa acccttatt ccggggtct ctcaaccag 
        ctgagcacc agccgcggg tgccaggcc ctgcgttat 
        ggggccact tgcgaggac atcttcaac gacctgaga 
        cggacaggg tcggtcttg cccggccag cacatttgc 
        gccacctgc agtgaggat ctatgcaac acagcgatg 
        gggctttcc cccggcttg tgtttaatg cttgtggtg 
        attttttta ctaatggcg ctgagagtg atttgtgcg 
        attttgggc gtttttctg gtgaataac ctcgattcg 
        gtgacgact ttgcaatgc tactcgtgc acagcatca 
        ctagggagc aattttaga tgcgagaac ttgtcctac 
        agcgggggt agtttaccc tactacacc tgcatgtac 
        ggcgatgca gtgtgtgcc aaatacata atcgaatat 
        ggtgattct ttgatgatc caccgaagt tgtcaacac 
        cgcgacatt tgtgccgtt ctagcctcg aaatacaac 
        aattattac agcaaattg ctcgattgt caaacttgt 
        tcggaccac gactggtgc aatggcgcc aacatttct 
        gactttaaa atgtctctg aaaatttgc atcctagtg 
        actttgctt ctcgcccat tacggggat tccctgaaa 
        tgctacacg tgtgaaacc caggattta aactcgccc 
        tgcgaggac ttgtcccga gggaaggac aaaatcaaa 
        acgcatgat tgttccgaa ggcgaagtt tgtgcagtt 
        tacaaaacc aaaaatccc tcaactgga atcatcaca 
        gtgaaaagg tcgtgcgaa ccgaaagac atttgtgag 
        gaattgtat gaagaaaat tccgatttt aaaaagttg 
        gagaaatgt aaaatttgt aaaacagat ctttgcgat 
        tccgggacg actttgtcc atctctgcg ataacgttc 
        atcctggcg ttgatgatc ggtgctttt attaattaa
      mRNA sequence
      • >TC034063 RA
        agtttaagt gagagacat ctcgcgcgc tcggagatt 
        ttttttcgc acagtgaag atgaagatg aagacggtt 
        ttgctcgtt tttggactt ttcgatctg ggctcctgc 
        atccgctgc tacgtgtgc acttcggag gcctccagc 
        aaccaatac gaatacgac gcgtgtctc tacggcgac 
        gagcgctac ctccaggcc gttgcgtgc cccgagccc 
        tccgtatgc accgcctac cactaccaa acccttatt 
        ccggggtct ctcaaccag ctgagcacc agccgcggg 
        tgccaggcc ctgcgttat ggggccact tgcgaggac 
        atcttcaac gacctgaga cggacaggg tcggtcttg 
        cccggccag cacatttgc gccacctgc agtgaggat 
        ctatgcaac acagcgatg gggctttcc cccggcttg 
        tgtttaatg cttgtggtg attttttta ctaatggcg 
        ctgagagtg atttgtgcg attttgggc gtttttctg 
        gtgaataac ctcgattcg gtgacgact ttgcaatgc 
        tactcgtgc acagcatca ctagggagc aattttaga 
        tgcgagaac ttgtcctac agcgggggt agtttaccc 
        tactacacc tgcatgtac ggcgatgca gtgtgtgcc 
        aaatacata atcgaatat ggtgattct ttgatgatc 
        caccgaagt tgtcaacac cgcgacatt tgtgccgtt 
        ctagcctcg aaatacaac aattattac agcaaattg 
        ctcgattgt caaacttgt tcggaccac gactggtgc 
        aatggcgcc aacatttct gactttaaa atgtctctg 
        aaaatttgc atcctagtg actttgctt ctcgcccat 
        tacggggat tccctgaaa tgctacacg tgtgaaacc 
        caggattta aactcgccc tgcgaggac ttgtcccga 
        gggaaggac aaaatcaaa acgcatgat tgttccgaa 
        ggcgaagtt tgtgcagtt tacaaaacc aaaaatccc 
        tcaactgga atcatcaca gtgaaaagg tcgtgcgaa 
        ccgaaagac atttgtgag gaattgtat gaagaaaat 
        tccgatttt aaaaagttg gagaaatgt aaaatttgt 
        aaaacagat ctttgcgat tccgggacg actttgtcc 
        atctctgcg ataacgttc atcctggcg ttgatgatc 
        ggtgctttt attaattaa caaattcag gtgcaataa 
        ttttattaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY