Status
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Gene information
General information
TC006390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8361064..8365938
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006390
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04129

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006390 RA
      MTTNVKLKE AFEVESKHG AFYTGGNIE WHQDTIYCQ 
      TPSAVTLLK IQDGVVCQT IGEENGEDC DTVQTFTTD 
      GERIISSHK SGLLKLWNG QGQLVKMWK YIHKGPIAK 
      LTLKDNLLA SGGSDGSVR IWDLDHQTC TLHLKGCQG 
      VVNIVEFHP IESVIFGSG DDGRILSWG LSKGEALIG 
      YNAHFSKVT SLVFANDAK HFVTSGRDK VIILWKFNQ 
      IKALRTIPL YEAVEVIVK LPEKFKLPD FKSDPDCVY 
      VASGGENGL VRVWDVTNS KEIYKQQNS LVSKAQDGG 
      LAITKLLLG DEDKTLAVI TADHNIILH ILKSFVCLK 
      QFIGFSDEI LDICFVGKS DSHLAVATN SCDIKLYDN 
      SDMNCQLLK GHSDIVLTL ATSRANPNL MLSGGKDNK 
      ICLWSLDGA IMSCVGTGL RHTGSVGSV TFSNINDFM 
      VSVSQDTCL KVWEVPKKP TSGTILNCT KTEIAHQKD 
      INCVSVSPN DKIIATASQ DKTAKLWTD SLTFVGTLK 
      GHKRGVWSV KFSPVDQVV VTSSADCMI KLWSVADLN 
      CLKTLEGHD SSVLKAEFL SNGMQILSA GADGLLKLF 
      SVKSSECVG TFEQHEGRI WAMAVRKDE SGVVTGGSD 
      SLLIKWKDV TEERKLQRL KEEEEEALQ QQKLANYLQ 
      NDELLKALK LALKLDRPL QVLKIVQGI IRKGETTGL 
      ADAVKGLKN EQKEALLKC ATTWNTNSR NCQSAQLVL 
      NILLGELQS GDFKPVGLG GTVEGALPY TERHFKRLT 
      QLLQDLHFV TYTINCMQP HIKNM
    CDS sequence
    • >TC006390 RA
      atgaccacc aatgtgaag ctcaaagag gcttttgaa 
      gtcgagtcc aaacatggc gctttctat acagggggc 
      aacatcgag tggcatcaa gacaccatc tactgtcaa 
      acgccttca gctgtcacg cttttgaag atccaagat 
      ggcgtcgtg tgccaaaca atcggcgaa gaaaatggc 
      gaagactgc gacacagtt caaactttt acaaccgat 
      ggcgaaaga atcatttcg tcacacaaa agcggtctt 
      ttaaaatta tggaatgga caaggccaa cttgtcaaa 
      atgtggaaa tacatccac aagggacct atcgcaaaa 
      ctaactctc aaagacaat ttactggca agtggtggt 
      tcggatgga agtgtgcga atatgggac ttggaccac 
      cagacttgc actttgcac ctgaaaggg tgccaaggc 
      gtggttaac atagtggaa ttccaccca atcgaaagt 
      gttattttc gggagtggg gacgatggg aggatcctg 
      tcgtggggc ttaagcaaa ggcgaggcg ttgattggc 
      tacaatgcg catttcagt aaagtgacg tcactcgtg 
      ttcgccaat gatgccaaa cactttgtg acgtcaggc 
      cgcgataag gttataatt ctatggaag tttaaccaa 
      atcaaagcc ttaaggact atacctttg tacgaagca 
      gtggaagtc atagtcaag ttacccgag aaatttaaa 
      ttacccgac tttaagtcg gaccctgac tgtgtttat 
      gtggcgtcc ggtggtgaa aatgggttg gtacgagtg 
      tgggacgtt accaactcg aaagaaata tacaagcag 
      caaaattcc ctagttagt aaagcgcaa gatggcggg 
      ttggccatc acgaagctg ttgctaggt gatgaggac 
      aaaacgttg gctgtgatc acagccgac cataacatt 
      attttacat atactcaag tcgttcgtt tgtttgaag 
      caattcata ggcttctct gatgagatt cttgatatt 
      tgtttcgtt ggcaaaagt gactcgcat ttggccgtg 
      gccacgaat tcgtgcgat atcaaactg tacgacaat 
      tctgacatg aactgtcag ttactgaaa ggtcacagt 
      gacattgtc ttgacctta gccactagt agggctaac 
      cccaacctt atgctttct ggtggtaaa gataacaag 
      atatgtcta tggtcactt gatggtgcc ataatgtcg 
      tgcgttggt accggtcta agacacacg ggttccgtg 
      ggttcggtc accttcagt aatatcaac gactttatg 
      gtaagtgtc agtcaagac acttgcctc aaagtctgg 
      gaagtcccg aagaagcca accagtggt accatccta 
      aactgtacc aagacagaa attgcgcac cagaaagac 
      atcaattgt gtgtccgtc tctccgaat gacaagatc 
      atagccacg gcctcccaa gacaaaacc gccaagctc 
      tggactgat agtttaacc ttcgtgggg acgctcaaa 
      gggcataaa cgtggggtc tggtcagtg aaattctcg 
      ccagttgat caagtcgtc gtaacctct tcggccgac 
      tgtatgatc aaactgtgg tcagtggct gacctcaac 
      tgcctcaaa acgctcgaa ggtcacgac tcgagtgtc 
      ctcaaagct gagttcctc tcaaacggg atgcagatt 
      ttgagtgcg ggggccgac ggcctcctc aaactcttc 
      agtgtgaag agttccgaa tgtgtcgga accttcgaa 
      cagcacgaa gggcggatc tgggccatg gcggtgcga 
      aaggacgaa agtggggtg gtgacggga gggtcggat 
      tcgctcctc atcaagtgg aaggacgtg acggaggaa 
      aggaagttg cagaggttg aaggaggaa gaagaggag 
      gcgttgcaa cagcaaaaa ttggccaat tatttgcaa 
      aatgacgag cttttgaaa gcgctaaag ttggccttg 
      aagcttgac cggcccttg caagtccta aagatcgtt 
      caagggatt atacgcaag ggtgagacg actggactc 
      gccgacgct gtgaagggg ttgaagaat gagcagaag 
      gaggcgctg ctcaagtgt gccacgact tggaatacc 
      aacagtcgg aactgtcaa tcggcacaa cttgtcttg 
      aatatactg ttaggggag ttgcagagt ggggatttt 
      aagcccgtg ggcttgggt gggaccgtt gagggcgct 
      ttgccctac actgagaga cacttcaag cgcttaacc 
      cagttgttg caagacttg catttcgtt acatatact 
      atcaattgt atgcagccc catataaaa aacatgtga
    mRNA sequence
    • >TC006390 RA
      acactcacg tgttttaca gggttaaag ctcactttt 
      taacaccaa agggtctct aataatgat tcatggact 
      tgtttttgc taatttatt gcaagccca aagtctatc 
      acttgttat attttgctt gttacgtaa tcgacagaa 
      tgagccaac ctcatggcg cgtcagctt ggtggaaca 
      actgtcaag tgttaaaac acatgttgt gctttagtg 
      ttatttttt ggacacaat tgtgcaagg aattagtgc 
      aatttatga taattgact ccattacgt gactaagta 
      aaaagtagt gttaaaatt tttggcaaa aaattgtga 
      agttgggtt ttttttcat caaggattt gcctaaaat 
      gaccaccaa tgtgaagct caaagaggc ttttgaagt 
      cgagtccaa acatggcgc tttctatac agggggcaa 
      catcgagtg gcatcaaga caccatcta ctgtcaaac 
      gccttcagc tgtcacgct tttgaagat ccaagatgg 
      cgtcgtgtg ccaaacaat cggcgaaga aaatggcga 
      agactgcga cacagttca aacttttac aaccgatgg 
      cgaaagaat catttcgtc acacaaaag cggtctttt 
      aaaattatg gaatggaca aggccaact tgtcaaaat 
      gtggaaata catccacaa gggacctat cgcaaaact 
      aactctcaa agacaattt actggcaag tggtggttc 
      ggatggaag tgtgcgaat atgggactt ggaccacca 
      gacttgcac tttgcacct gaaagggtg ccaaggcgt 
      ggttaacat agtggaatt ccacccaat cgaaagtgt 
      tattttcgg gagtgggga cgatgggag gatcctgtc 
      gtggggctt aagcaaagg cgaggcgtt gattggcta 
      caatgcgca tttcagtaa agtgacgtc actcgtgtt 
      cgccaatga tgccaaaca ctttgtgac gtcaggccg 
      cgataaggt tataattct atggaagtt taaccaaat 
      caaagcctt aaggactat acctttgta cgaagcagt 
      ggaagtcat agtcaagtt acccgagaa atttaaatt 
      acccgactt taagtcgga ccctgactg tgtttatgt 
      ggcgtccgg tggtgaaaa tgggttggt acgagtgtg 
      ggacgttac caactcgaa agaaatata caagcagca 
      aaattccct agttagtaa agcgcaaga tggcgggtt 
      ggccatcac gaagctgtt gctaggtga tgaggacaa 
      aacgttggc tgtgatcac agccgacca taacattat 
      tttacatat actcaagtc gttcgtttg tttgaagca 
      attcatagg cttctctga tgagattct tgatatttg 
      tttcgttgg caaaagtga ctcgcattt ggccgtggc 
      cacgaattc gtgcgatat caaactgta cgacaattc 
      tgacatgaa ctgtcagtt actgaaagg tcacagtga 
      cattgtctt gaccttagc cactagtag ggctaaccc 
      caaccttat gctttctgg tggtaaaga taacaagat 
      atgtctatg gtcacttga tggtgccat aatgtcgtg 
      cgttggtac cggtctaag acacacggg ttccgtggg 
      ttcggtcac cttcagtaa tatcaacga ctttatggt 
      aagtgtcag tcaagacac ttgcctcaa agtctggga 
      agtcccgaa gaagccaac cagtggtac catcctaaa 
      ctgtaccaa gacagaaat tgcgcacca gaaagacat 
      caattgtgt gtccgtctc tccgaatga caagatcat 
      agccacggc ctcccaaga caaaaccgc caagctctg 
      gactgatag tttaacctt cgtggggac gctcaaagg 
      gcataaacg tggggtctg gtcagtgaa attctcgcc 
      agttgatca agtcgtcgt aacctcttc ggccgactg 
      tatgatcaa actgtggtc agtggctga cctcaactg 
      cctcaaaac gctcgaagg tcacgactc gagtgtcct 
      caaagctga gttcctctc aaacgggat gcagatttt 
      gagtgcggg ggccgacgg cctcctcaa actcttcag 
      tgtgaagag ttccgaatg tgtcggaac cttcgaaca 
      gcacgaagg gcggatctg ggccatggc ggtgcgaaa 
      ggacgaaag tggggtggt gacgggagg gtcggattc 
      gctcctcat caagtggaa ggacgtgac ggaggaaag 
      gaagttgca gaggttgaa ggaggaaga agaggaggc 
      gttgcaaca gcaaaaatt ggccaatta tttgcaaaa 
      tgacgagct tttgaaagc gctaaagtt ggccttgaa 
      gcttgaccg gcccttgca agtcctaaa gatcgttca 
      agggattat acgcaaggg tgagacgac tggactcgc 
      cgacgctgt gaaggggtt gaagaatga gcagaagga 
      ggcgctgct caagtgtgc cacgacttg gaataccaa 
      cagtcggaa ctgtcaatc ggcacaact tgtcttgaa 
      tatactgtt aggggagtt gcagagtgg ggattttaa 
      gcccgtggg cttgggtgg gaccgttga gggcgcttt 
      gccctacac tgagagaca cttcaagcg cttaaccca 
      gttgttgca agacttgca tttcgttac atatactat 
      caattgtat gcagcccca tataaaaaa catgtgaat 
      gtcttgttg ggttttgtc attatcggt attgttttt 
      agtgagttc ttgacttgt ttaagtaac gtcattgtc 
      aaataattg agtcagtgt gatttaaac atttgtttt 
      ttcaattaa atcgtttca tgatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY