| General information | |||
| TC006390 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 8361064..8365938 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006390 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04129 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC006390 RA
MTTNVKLKE AFEVESKHG AFYTGGNIE WHQDTIYCQ TPSAVTLLK IQDGVVCQT IGEENGEDC DTVQTFTTD GERIISSHK SGLLKLWNG QGQLVKMWK YIHKGPIAK LTLKDNLLA SGGSDGSVR IWDLDHQTC TLHLKGCQG VVNIVEFHP IESVIFGSG DDGRILSWG LSKGEALIG YNAHFSKVT SLVFANDAK HFVTSGRDK VIILWKFNQ IKALRTIPL YEAVEVIVK LPEKFKLPD FKSDPDCVY VASGGENGL VRVWDVTNS KEIYKQQNS LVSKAQDGG LAITKLLLG DEDKTLAVI TADHNIILH ILKSFVCLK QFIGFSDEI LDICFVGKS DSHLAVATN SCDIKLYDN SDMNCQLLK GHSDIVLTL ATSRANPNL MLSGGKDNK ICLWSLDGA IMSCVGTGL RHTGSVGSV TFSNINDFM VSVSQDTCL KVWEVPKKP TSGTILNCT KTEIAHQKD INCVSVSPN DKIIATASQ DKTAKLWTD SLTFVGTLK GHKRGVWSV KFSPVDQVV VTSSADCMI KLWSVADLN CLKTLEGHD SSVLKAEFL SNGMQILSA GADGLLKLF SVKSSECVG TFEQHEGRI WAMAVRKDE SGVVTGGSD SLLIKWKDV TEERKLQRL KEEEEEALQ QQKLANYLQ NDELLKALK LALKLDRPL QVLKIVQGI IRKGETTGL ADAVKGLKN EQKEALLKC ATTWNTNSR NCQSAQLVL NILLGELQS GDFKPVGLG GTVEGALPY TERHFKRLT QLLQDLHFV TYTINCMQP HIKNM
- >TC006390 RA
atgaccacc aatgtgaag ctcaaagag gcttttgaa gtcgagtcc aaacatggc gctttctat acagggggc aacatcgag tggcatcaa gacaccatc tactgtcaa acgccttca gctgtcacg cttttgaag atccaagat ggcgtcgtg tgccaaaca atcggcgaa gaaaatggc gaagactgc gacacagtt caaactttt acaaccgat ggcgaaaga atcatttcg tcacacaaa agcggtctt ttaaaatta tggaatgga caaggccaa cttgtcaaa atgtggaaa tacatccac aagggacct atcgcaaaa ctaactctc aaagacaat ttactggca agtggtggt tcggatgga agtgtgcga atatgggac ttggaccac cagacttgc actttgcac ctgaaaggg tgccaaggc gtggttaac atagtggaa ttccaccca atcgaaagt gttattttc gggagtggg gacgatggg aggatcctg tcgtggggc ttaagcaaa ggcgaggcg ttgattggc tacaatgcg catttcagt aaagtgacg tcactcgtg ttcgccaat gatgccaaa cactttgtg acgtcaggc cgcgataag gttataatt ctatggaag tttaaccaa atcaaagcc ttaaggact atacctttg tacgaagca gtggaagtc atagtcaag ttacccgag aaatttaaa ttacccgac tttaagtcg gaccctgac tgtgtttat gtggcgtcc ggtggtgaa aatgggttg gtacgagtg tgggacgtt accaactcg aaagaaata tacaagcag caaaattcc ctagttagt aaagcgcaa gatggcggg ttggccatc acgaagctg ttgctaggt gatgaggac aaaacgttg gctgtgatc acagccgac cataacatt attttacat atactcaag tcgttcgtt tgtttgaag caattcata ggcttctct gatgagatt cttgatatt tgtttcgtt ggcaaaagt gactcgcat ttggccgtg gccacgaat tcgtgcgat atcaaactg tacgacaat tctgacatg aactgtcag ttactgaaa ggtcacagt gacattgtc ttgacctta gccactagt agggctaac cccaacctt atgctttct ggtggtaaa gataacaag atatgtcta tggtcactt gatggtgcc ataatgtcg tgcgttggt accggtcta agacacacg ggttccgtg ggttcggtc accttcagt aatatcaac gactttatg gtaagtgtc agtcaagac acttgcctc aaagtctgg gaagtcccg aagaagcca accagtggt accatccta aactgtacc aagacagaa attgcgcac cagaaagac atcaattgt gtgtccgtc tctccgaat gacaagatc atagccacg gcctcccaa gacaaaacc gccaagctc tggactgat agtttaacc ttcgtgggg acgctcaaa gggcataaa cgtggggtc tggtcagtg aaattctcg ccagttgat caagtcgtc gtaacctct tcggccgac tgtatgatc aaactgtgg tcagtggct gacctcaac tgcctcaaa acgctcgaa ggtcacgac tcgagtgtc ctcaaagct gagttcctc tcaaacggg atgcagatt ttgagtgcg ggggccgac ggcctcctc aaactcttc agtgtgaag agttccgaa tgtgtcgga accttcgaa cagcacgaa gggcggatc tgggccatg gcggtgcga aaggacgaa agtggggtg gtgacggga gggtcggat tcgctcctc atcaagtgg aaggacgtg acggaggaa aggaagttg cagaggttg aaggaggaa gaagaggag gcgttgcaa cagcaaaaa ttggccaat tatttgcaa aatgacgag cttttgaaa gcgctaaag ttggccttg aagcttgac cggcccttg caagtccta aagatcgtt caagggatt atacgcaag ggtgagacg actggactc gccgacgct gtgaagggg ttgaagaat gagcagaag gaggcgctg ctcaagtgt gccacgact tggaatacc aacagtcgg aactgtcaa tcggcacaa cttgtcttg aatatactg ttaggggag ttgcagagt ggggatttt aagcccgtg ggcttgggt gggaccgtt gagggcgct ttgccctac actgagaga cacttcaag cgcttaacc cagttgttg caagacttg catttcgtt acatatact atcaattgt atgcagccc catataaaa aacatgtga
- >TC006390 RA
acactcacg tgttttaca gggttaaag ctcactttt taacaccaa agggtctct aataatgat tcatggact tgtttttgc taatttatt gcaagccca aagtctatc acttgttat attttgctt gttacgtaa tcgacagaa tgagccaac ctcatggcg cgtcagctt ggtggaaca actgtcaag tgttaaaac acatgttgt gctttagtg ttatttttt ggacacaat tgtgcaagg aattagtgc aatttatga taattgact ccattacgt gactaagta aaaagtagt gttaaaatt tttggcaaa aaattgtga agttgggtt ttttttcat caaggattt gcctaaaat gaccaccaa tgtgaagct caaagaggc ttttgaagt cgagtccaa acatggcgc tttctatac agggggcaa catcgagtg gcatcaaga caccatcta ctgtcaaac gccttcagc tgtcacgct tttgaagat ccaagatgg cgtcgtgtg ccaaacaat cggcgaaga aaatggcga agactgcga cacagttca aacttttac aaccgatgg cgaaagaat catttcgtc acacaaaag cggtctttt aaaattatg gaatggaca aggccaact tgtcaaaat gtggaaata catccacaa gggacctat cgcaaaact aactctcaa agacaattt actggcaag tggtggttc ggatggaag tgtgcgaat atgggactt ggaccacca gacttgcac tttgcacct gaaagggtg ccaaggcgt ggttaacat agtggaatt ccacccaat cgaaagtgt tattttcgg gagtgggga cgatgggag gatcctgtc gtggggctt aagcaaagg cgaggcgtt gattggcta caatgcgca tttcagtaa agtgacgtc actcgtgtt cgccaatga tgccaaaca ctttgtgac gtcaggccg cgataaggt tataattct atggaagtt taaccaaat caaagcctt aaggactat acctttgta cgaagcagt ggaagtcat agtcaagtt acccgagaa atttaaatt acccgactt taagtcgga ccctgactg tgtttatgt ggcgtccgg tggtgaaaa tgggttggt acgagtgtg ggacgttac caactcgaa agaaatata caagcagca aaattccct agttagtaa agcgcaaga tggcgggtt ggccatcac gaagctgtt gctaggtga tgaggacaa aacgttggc tgtgatcac agccgacca taacattat tttacatat actcaagtc gttcgtttg tttgaagca attcatagg cttctctga tgagattct tgatatttg tttcgttgg caaaagtga ctcgcattt ggccgtggc cacgaattc gtgcgatat caaactgta cgacaattc tgacatgaa ctgtcagtt actgaaagg tcacagtga cattgtctt gaccttagc cactagtag ggctaaccc caaccttat gctttctgg tggtaaaga taacaagat atgtctatg gtcacttga tggtgccat aatgtcgtg cgttggtac cggtctaag acacacggg ttccgtggg ttcggtcac cttcagtaa tatcaacga ctttatggt aagtgtcag tcaagacac ttgcctcaa agtctggga agtcccgaa gaagccaac cagtggtac catcctaaa ctgtaccaa gacagaaat tgcgcacca gaaagacat caattgtgt gtccgtctc tccgaatga caagatcat agccacggc ctcccaaga caaaaccgc caagctctg gactgatag tttaacctt cgtggggac gctcaaagg gcataaacg tggggtctg gtcagtgaa attctcgcc agttgatca agtcgtcgt aacctcttc ggccgactg tatgatcaa actgtggtc agtggctga cctcaactg cctcaaaac gctcgaagg tcacgactc gagtgtcct caaagctga gttcctctc aaacgggat gcagatttt gagtgcggg ggccgacgg cctcctcaa actcttcag tgtgaagag ttccgaatg tgtcggaac cttcgaaca gcacgaagg gcggatctg ggccatggc ggtgcgaaa ggacgaaag tggggtggt gacgggagg gtcggattc gctcctcat caagtggaa ggacgtgac ggaggaaag gaagttgca gaggttgaa ggaggaaga agaggaggc gttgcaaca gcaaaaatt ggccaatta tttgcaaaa tgacgagct tttgaaagc gctaaagtt ggccttgaa gcttgaccg gcccttgca agtcctaaa gatcgttca agggattat acgcaaggg tgagacgac tggactcgc cgacgctgt gaaggggtt gaagaatga gcagaagga ggcgctgct caagtgtgc cacgacttg gaataccaa cagtcggaa ctgtcaatc ggcacaact tgtcttgaa tatactgtt aggggagtt gcagagtgg ggattttaa gcccgtggg cttgggtgg gaccgttga gggcgcttt gccctacac tgagagaca cttcaagcg cttaaccca gttgttgca agacttgca tttcgttac atatactat caattgtat gcagcccca tataaaaaa catgtgaat gtcttgttg ggttttgtc attatcggt attgttttt agtgagttc ttgacttgt ttaagtaac gtcattgtc aaataattg agtcagtgt gatttaaac atttgtttt ttcaattaa atcgtttca tgatta
