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Gene information
General information
TC006389 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8356483..8357890
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006389
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04128

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006389 RA
      MQWSHNTCR ICGQISDTC HLIWAKSPN DIENKIKKC 
      LKLELFKDD FKPKQLCNP CLLKLDDFY QFLVICHAT 
      DQKFESILM NRQEWGPET ILDYTQQKM SVIVSNKGG 
      LEHLECNEI DLYKESVLP LETCPEMLD IVENANYMP 
      ENLDYLNPN YGMKKFHDF YQEPGVLRH SSNYPQEAA 
      INYSCQQET FPDLSYPNV GLDYSNYAP QPNFAEAKA 
      EPSRKPPAS QKTFPCPHC EKKFKRRVT LNAHIAIHT 
      KIRPYICDI CKKSYAIKC DLTNHLKIH AGRNKCKYC 
      SSAFPAPSK LQRHLRTHT KDRPFVCDF KNCGKSFSD 
      KRNLDGHKA LHSNEFNFK CQECGRAFR TKNRLKQHE 
      KAHTVATPY VCEICSKAY KYKSTLIFH LKKHNGYFC 
      PYCEKDCEK AVKLLRHKK VCAVARNKN
    CDS sequence
    • >TC006389 RA
      atgcagtgg agtcacaac acgtgccgc atttgtggc 
      caaatcagc gacacgtgc cacctcatt tgggccaag 
      agccccaac gatattgag aataaaata aaaaaatgt 
      ctaaagttg gagcttttc aaggatgat ttcaaaccg 
      aaacagttg tgtaaccct tgtttgtta aaactggac 
      gatttttat caattccta gtgatatgt cacgccact 
      gaccagaaa ttcgagtcg attttaatg aaccggcaa 
      gagtggggc cctgagaca atcctcgac tacacgcag 
      caaaaaatg agcgtgatc gtgtcaaat aaggggggc 
      ttggagcac ctcgagtgc aacgaaatc gatttatac 
      aaggagtca gttttgccc ctggagacg tgccccgaa 
      atgttggat attgtggaa aacgcgaac tacatgccg 
      gagaatctc gattatttg aaccccaat tacgggatg 
      aagaagttt catgatttc taccaagag ccgggcgtt 
      ttgaggcac tcctcaaac tacccccag gaggctgcg 
      atcaattac agttgccaa caagagacg ttccccgac 
      ctcagttac cccaatgtg gggctggac tactcgaac 
      tacgccccc cagccgaat ttcgccgag gcgaaagcc 
      gagccatca cgcaagccc cctgcgagt caaaagacc 
      ttcccgtgc ccccattgc gagaaaaaa ttcaaacgc 
      agggtcaca ctcaacgcc cacatagcc atacacacc 
      aaaatacgc ccttatatc tgtgatatt tgcaaaaaa 
      tcgtacgca attaaatgc gacttaaca aaccatctt 
      aaaattcac gccggtcgt aataaatgc aaatattgc 
      tcgagcgcg ttcccggcc cccagcaag ttgcaaagg 
      cacttgcgg acgcacacg aaagatcgg ccgtttgtc 
      tgcgatttt aaaaattgc ggcaagtcg ttctcggat 
      aagaggaac ttggacggg cataaagcc ctgcattcg 
      aacgagttt aatttcaag tgccaggag tgtggaaga 
      gccttccgg acgaaaaat cggctcaaa cagcatgag 
      aaggcgcac acggtggct acgccctac gtgtgcgag 
      atttgctct aaggcgtac aagtacaag tcgactctt 
      atttttcac ttgaagaaa cacaacggc tatttttgt 
      ccctactgt gagaaggac tgcgaaaag gcggtcaag 
      ttgttgagg cacaagaaa gtgtgcgcc gtcgcccga 
      aataaaaat taa
    mRNA sequence
    • >TC006389 RA
      gggggtgca cccaaaaat gcagtggag tcacaacac 
      gtgccgcat ttgtggcca aatcagcga cacgtgcca 
      cctcatttg ggccaagag ccccaacga tattgagaa 
      taaaataaa aaaatgtct aaagttgga gcttttcaa 
      ggatgattt caaaccgaa acagttgtg taacccttg 
      tttgttaaa actggacga tttttatca attcctagt 
      gatatgtca cgccactga ccagaaatt cgagtcgat 
      tttaatgaa ccggcaaga gtggggccc tgagacaat 
      cctcgacta cacgcagca aaaaatgag cgtgatcgt 
      gtcaaataa ggggggctt ggagcacct cgagtgcaa 
      cgaaatcga tttatacaa ggagtcagt tttgcccct 
      ggagacgtg ccccgaaat gttggatat tgtggaaaa 
      cgcgaacta catgccgga gaatctcga ttatttgaa 
      ccccaatta cgggatgaa gaagtttca tgatttcta 
      ccaagagcc gggcgtttt gaggcactc ctcaaacta 
      cccccagga ggctgcgat caattacag ttgccaaca 
      agagacgtt ccccgacct cagttaccc caatgtggg 
      gctggacta ctcgaacta cgcccccca gccgaattt 
      cgccgaggc gaaagccga gccatcacg caagccccc 
      tgcgagtca aaagacctt cccgtgccc ccattgcga 
      gaaaaaatt caaacgcag ggtcacact caacgccca 
      catagccat acacaccaa aatacgccc ttatatctg 
      tgatatttg caaaaaatc gtacgcaat taaatgcga 
      cttaacaaa ccatcttaa aattcacgc cggtcgtaa 
      taaatgcaa atattgctc gagcgcgtt cccggcccc 
      cagcaagtt gcaaaggca cttgcggac gcacacgaa 
      agatcggcc gtttgtctg cgattttaa aaattgcgg 
      caagtcgtt ctcggataa gaggaactt ggacgggca 
      taaagccct gcattcgaa cgagtttaa tttcaagtg 
      ccaggagtg tggaagagc cttccggac gaaaaatcg 
      gctcaaaca gcatgagaa ggcgcacac ggtggctac 
      gccctacgt gtgcgagat ttgctctaa ggcgtacaa 
      gtacaagtc gactcttat ttttcactt gaagaaaca 
      caacggcta tttttgtcc ctactgtga gaaggactg 
      cgaaaaggc ggtcaagtt gttgaggca caagaaagt 
      gtgcgccgt cgcccgaaa taaaaatta atttaacgc 
      ttgttgtga gtacggtat ttttttctc tcgcccagt 
      gttgtgtat gaataaaag tagggatct agg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY