Status
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Gene information
General information
TC006380 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8191651..8197094
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006380
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04127

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006380 RA
      MFATNLSFK LLKTLTTSN CNVSRWSAL EKIFNTKPG 
      LNLDIVTKN VGLFGVPQL TTHEGFYYV TQKCVEKTD 
      DLVREALSS DRKRKMVEI FDELSDSLC QVADLAEFI 
      RFAHPSSEY IAAAEHTSV AISKLVERL NTNIQMYEA 
      LKTAVNTTD ILPTTEVDN LVGKLFLFD FEQCGIHLN 
      EHKRNQVVE LNNKILCAG QQFLNGISN PKVIKQSAL 
      PDSVRDVFS SDGDKVVIS GLYADSSNP LVREMAYKI 
      FLYPDPNQE YLLNELLSS RHELALACD FDTYSERAL 
      RGSILDKPP KVMELLEKI SESIHSKAM ADFSDMAHL 
      KMKENPNGG PLAVWDVPY FTQKAKRDR FEVTSKEYS 
      PYFSLGACM EGLNLIFNA LYGISLVNS EIASGETWS 
      KDVYKLSVV HESEGLLGY IYCDFYERN FKPNQECHF 
      TIRGGKVLP DGTYQLPIV VLMLNFPLP RWSSPTLLS 
      PNMVDNLFH EMGHAMHSM LARTQYQHV SGTRCSTDF 
      AEVPSILME YFASDPRVI KLFAKHFQT KEPIPEPML 
      VKLCASKTL FSASETQLQ IFYAALDQI YHGRHPLSG 
      TTTEILAQT QNRFYGLPY VANTTWQLR FSHLVGYGA 
      KYYSYLVSR ALAYLIWET YFKVDPLSR VQGDRYRRE 
      CLAHGGGKN PSDLIGDYL KVTPTPGLL ANSLNSEIE 
      SSQSQLNEM RNYCNKL
    CDS sequence
    • >TC006380 RA
      atgtttgcc acgaattta tcattcaag ctcctcaaa 
      actctcaca acctcaaac tgcaatgtt agtcgatgg 
      tcagctcta gaaaaaata tttaacacc aaaccgggc 
      ttaaacctt gacatagtg acaaaaaat gttggcctc 
      tttggggtg ccccaactc accacccat gagggtttt 
      tactacgtt acgcaaaaa tgcgttgaa aaaaccgac 
      gatttggtg cgtgaggcc ctttcgagc gaccgaaag 
      cgcaaaatg gttgagatt tttgacgaa ttgtctgac 
      tctttgtgc caagtggct gatttggct gagtttatt 
      cgatttgca cacccgtct tctgagtac atcgcggca 
      gcggagcac acgtcagtt gccataagt aaactagtc 
      gaaagatta aacactaat attcaaatg tatgaggct 
      ctaaagact gctgtgaat acaacagat atactgcca 
      acaaccgag gttgataac ctggtcggg aagttgttt 
      ttgtttgat tttgaacag tgtgggatt cacctaaat 
      gagcacaag cgaaaccaa gttgttgaa cttaataac 
      aaaatttta tgtgcgggg caacagttc ctcaatgga 
      atatcaaat cctaaggtt atcaagcag agtgccctt 
      ccggacagc gtccgcgat gtattttcg agcgatggc 
      gacaaagtc gttatttcc ggcctctac gccgactcc 
      tccaacccc ctcgtgcgt gaaatggcc tacaagata 
      ttcctctac cccgacccc aatcaggaa tacctcctc 
      aacgagttg ttatcttcg cgacacgag ctcgcccta 
      gcgtgtgat tttgacact tacagcgaa cgcgccctc 
      cgtggtagc atcctagac aaacccccc aaagtcatg 
      gagttactc gaaaaaatc tccgaaagt atccatagc 
      aaagcaatg gccgatttc tccgacatg gcccacttg 
      aaaatgaag gagaaccca aacgggggg ccactcgca 
      gtttgggac gtcccgtac ttcacccaa aaagccaaa 
      agggaccgg tttgaggtc actagcaag gaatactcc 
      ccgtatttt tcgctcggc gcttgcatg gagggcctt 
      aacttgatt ttcaatgct ttgtacggc atatctcta 
      gttaactcg gaaatcgcc tcgggtgag acatggtcc 
      aaagatgtt tataaacta tcagtggtg cacgagagt 
      gagggcttg ctggggtac atatattgc gatttctac 
      gaacgtaac ttcaagccc aatcaagag tgccacttt 
      accatccgt gggggcaaa gtcttgccc gatggtacc 
      taccagttg cccatcgtg gtcctcatg ttgaatttc 
      ccgctgcca cggtggtcc agccctacc ctactttcg 
      cccaacatg gtggacaac ctgttccat gaaatggga 
      cacgcgatg cattcaatg ttggccaga acccagtac 
      cagcacgtt tctggtacc cgctgtagt accgacttt 
      gccgaagta ccatcgatt ctgatggaa tatttcgca 
      tcggacccc cgagttatc aaactcttt gccaaacat 
      ttccaaacc aaagaaccc atccctgaa cccatgctg 
      gtcaagctg tgcgcctcg aagaccctc tttagtgcc 
      agcgagacc cagctgcag attttctat gccgccttg 
      gaccagatc taccatggg agacaccca ctgagtggt 
      accaccacc gaaattttg gcgcagacc cagaacagg 
      ttctatggg ttgccgtat gtggcaaat accacgtgg 
      cagttgagg tttagtcat ttggtgggt tatggggca 
      aagtactac tcgtatcta gtgtcaagg gctttggct 
      tacttgatt tgggagacg tattttaaa gttgatccg 
      ttgagtcgt gtccaaggt gacaggtac cggagggag 
      tgtctggcg catggtggg ggtaaaaat ccctccgat 
      ctgattggc gattatttg aaggtcacc cccactccc 
      ggcctactg gccaatagt ttgaatagt gaaattgaa 
      agtagccaa agccaactg aacgaaatg cgaaattat 
      tgcaataaa ttatga
    mRNA sequence
    • >TC006380 RA
      cccaaaatc acaatcatc tgtcaaaaa aagccaaag 
      ttcttgtaa ctttaaaaa acccatttt tgcataatt 
      ttcacccaa atcccattt taattaacc gttatgtac 
      aatgtctca cacaaaaac atcattttc tattttacg 
      gaacgttaa atcttgcta aaaccacat cagacgtca 
      cgttacaaa cgcaacttt gaaaatgtt tgccacgaa 
      tttatcatt caagctcct caaaactct cacaacctc 
      aaactgcaa tgttagtcg atggtcagc tctagaaaa 
      aatatttaa caccaaacc gggcttaaa ccttgacat 
      agtgacaaa aaatgttgg cctctttgg ggtgcccca 
      actcaccac ccatgaggg tttttacta cgttacgca 
      aaaatgcgt tgaaaaaac cgacgattt ggtgcgtga 
      ggccctttc gagcgaccg aaagcgcaa aatggttga 
      gatttttga cgaattgtc tgactcttt gtgccaagt 
      ggctgattt ggctgagtt tattcgatt tgcacaccc 
      gtcttctga gtacatcgc ggcagcgga gcacacgtc 
      agttgccat aagtaaact agtcgaaag attaaacac 
      taatattca aatgtatga ggctctaaa gactgctgt 
      gaatacaac agatatact gccaacaac cgaggttga 
      taacctggt cgggaagtt gtttttgtt tgattttga 
      acagtgtgg gattcacct aaatgagca caagcgaaa 
      ccaagttgt tgaacttaa taacaaaat tttatgtgc 
      ggggcaaca gttcctcaa tggaatatc aaatcctaa 
      ggttatcaa gcagagtgc ccttccgga cagcgtccg 
      cgatgtatt ttcgagcga tggcgacaa agtcgttat 
      ttccggcct ctacgccga ctcctccaa ccccctcgt 
      gcgtgaaat ggcctacaa gatattcct ctaccccga 
      ccccaatca ggaatacct cctcaacga gttgttatc 
      ttcgcgaca cgagctcgc cctagcgtg tgattttga 
      cacttacag cgaacgcgc cctccgtgg tagcatcct 
      agacaaacc ccccaaagt catggagtt actcgaaaa 
      aatctccga aagtatcca tagcaaagc aatggccga 
      tttctccga catggccca cttgaaaat gaaggagaa 
      cccaaacgg ggggccact cgcagtttg ggacgtccc 
      gtacttcac ccaaaaagc caaaaggga ccggtttga 
      ggtcactag caaggaata ctccccgta tttttcgct 
      cggcgcttg catggaggg ccttaactt gattttcaa 
      tgctttgta cggcatatc tctagttaa ctcggaaat 
      cgcctcggg tgagacatg gtccaaaga tgtttataa 
      actatcagt ggtgcacga gagtgaggg cttgctggg 
      gtacatata ttgcgattt ctacgaacg taacttcaa 
      gcccaatca agagtgcca ctttaccat ccgtggggg 
      caaagtctt gcccgatgg tacctacca gttgcccat 
      cgtggtcct catgttgaa tttcccgct gccacggtg 
      gtccagccc taccctact ttcgcccaa catggtgga 
      caacctgtt ccatgaaat gggacacgc gatgcattc 
      aatgttggc cagaaccca gtaccagca cgtttctgg 
      tacccgctg tagtaccga ctttgccga agtaccatc 
      gattctgat ggaatattt cgcatcgga cccccgagt 
      tatcaaact ctttgccaa acatttcca aaccaaaga 
      acccatccc tgaacccat gctggtcaa gctgtgcgc 
      ctcgaagac cctctttag tgccagcga gacccagct 
      gcagatttt ctatgccgc cttggacca gatctacca 
      tgggagaca cccactgag tggtaccac caccgaaat 
      tttggcgca gacccagaa caggttcta tgggttgcc 
      gtatgtggc aaataccac gtggcagtt gaggtttag 
      tcatttggt gggttatgg ggcaaagta ctactcgta 
      tctagtgtc aagggcttt ggcttactt gatttggga 
      gacgtattt taaagttga tccgttgag tcgtgtcca 
      aggtgacag gtaccggag ggagtgtct ggcgcatgg 
      tgggggtaa aaatccctc cgatctgat tggcgatta 
      tttgaaggt cacccccac tcccggcct actggccaa 
      tagtttgaa tagtgaaat tgaaagtag ccaaagcca 
      actgaacga aatgcgaaa ttattgcaa taaattatg 
      attactata gtaatgtgc agttatttt attcgcggc 
      ctacttttt tttgataag agagctaaa acacgggaa 
      aaatgcaaa attagtgta attattata gcgatttaa 
      ttttgtgat ttttctgtt attttgttg atatctgtg 
      attttaatt caaaaaacg cattcaaag gcttgaaaa 
      atgtataaa taatattgt cttcgtcat ttttagttt 
      ttttttgct tattttcga taaaatttc gtttaggaa 
      cgagctaat tcgagtgaa atcggcaaa ttctcatca 
      atttcgatc taatttgtt gatatttta ccgaaattt 
      tttaactga tagaagagc tataacaaa aaatgcaaa 
      attagtgta attatttgg tgtttttta attgtttta 
      aagagtggc gttgagggt gacaggtcc cgagggagt 
      gtctggcgc atggtgggg gtaaaactc ccttcgaac 
      tgattggag attatttga aggtcaccc ccaccccgg 
      cccactggc caatagttt gcatattga aactgaaag 
      tagccaaag ccaactgag cgaaatgcg aaattattg 
      caataaatt atgataact ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY