Status
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Gene information
General information
TC006378 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8177141..8182180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006378
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04126

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006378 RA
      MDETTKNRG KWGNSIEFL MSCIAMNVG LGNIWRFPF 
      VAYENGGGA FLIPYISVL ILLGRPMYY LEMCLGQFS 
      SRGNVEMFE SIAQVLKGV GFGQLIGTF SVATYYCSL 
      MALTLYYLI HSFRPDLPW SQCDPNWGR DTWLGNNTC 
      IPSKSTEVG VENSVSSAE AWFRIEVLR EATDLSHGI 
      GYPNWELTL CLLCSWIIT FCICAKGVQ SSGKAAYFL 
      AIFPFIILF ALLIRSVTL EGAGQGILY FVKPNWEKL 
      LSAQVWYAA VTQCFFSLN IGFGSVTMY ASYNSFDHN 
      VYRDAMVVT TLDTFTSFL SGLIIFGIL GNLAYKMNV 
      EVSDVVKSG GTGLAFISY PEAIAHFDI VPWLFAILF 
      FFMLFVLGV GSLIALKGC AFTVILDAF PHLKTWHVS 
      FGTALVGFL VGLVYITPG GQFIFTMVD FFGGTFIFY 
      VLTIIEVLS VMWWYGLEN ICLDIEFMS KKRPGPYWR 
      ICWAVIIPI VLITVFVYF IATLEELKY EDQSYPGYI 
      IVWGWALFG VGVMQPLIW WGIEIGKNY KREGVSKAI 
      LRTFKHENW GPKEEEFYN NWVEFKRER RQKTATGRH 
      WFKHKLCVL VGK
    CDS sequence
    • >TC006378 RA
      atggacgaa acgacgaaa aacaggggc aaatggggc 
      aacagcatc gagttccta atgtcctgc attgccatg 
      aacgtgggc ttgggcaac atttggcgt ttccccttc 
      gtggcgtac gaaaacggc ggtggcgcc ttcctcatc 
      ccctacatc tccgtccta atcctcctc ggccgcccc 
      atgtactac ctggaaatg tgcctcggc cagttcagc 
      agccgaggc aacgtcgaa atgttcgag tccatagcc 
      caggtcttg aaaggggtc ggttttggg caactaatc 
      gggacgttt tccgtggcc acctactac tgctccctg 
      atggccttg accctgtac tacttgatc cattcgttc 
      cggcccgac ctgccctgg tcgcagtgc gaccccaac 
      tggggtcgg gacacgtgg ctgggcaac aatacgtgc 
      attccgagc aagagcacg gaagtgggg gtcgagaat 
      tcggtcagc tcggctgaa gcctggttc aggatcgag 
      gtgttgagg gaagccacg gatttgtcg catgggata 
      gggtacccc aattgggag ttgacgctc tgcttgctc 
      tgctcgtgg attatcacg ttttgtatt tgcgccaaa 
      ggggtgcaa agctcgggg aaagcggcg tattttctt 
      gcgattttt ccatttatt atacttttc gcgcttttg 
      attcggtcg gttacgctg gagggggct ggacaaggg 
      attttgtat tttgtcaag cccaactgg gaaaagtta 
      ctaagtgca caggtatgg tatgcagcc gtcactcaa 
      tgtttcttc tccttgaac atcggtttc ggttcggtt 
      acaatgtac gcatcttat aacagcttc gatcacaat 
      gtttacaga gacgcaatg gtggtcaca actcttgac 
      actttcact tcatttcta tccggtttg ataattttc 
      gggatttta ggaaattta gcttacaag atgaacgta 
      gaagtgtca gatgttgtt aaatctggt ggaacaggt 
      ttagctttt atctcatac cccgaagct atagctcat 
      tttgatatt gtaccatgg ttgtttgct attttgttt 
      ttcttcatg ttgtttgta ctgggcgtt ggaagttta 
      atagcgtta aaagggtgc gcttttacg gtgattttg 
      gacgctttt ccccatttg aaaacatgg catgtgtca 
      tttggaacg gctttggtt ggctttctg gtcggttta 
      gtctacatc accccgggt ggccaattt attttcaca 
      atggtggat tttttcggc ggtactttc attttctac 
      gtcctgacc ataatcgaa gtcctttcg gtgatgtgg 
      tggtacgga ttggaaaat atctgcctc gacattgag 
      tttatgtca aaaaaacgt ccagggccg tactggcga 
      atttgctgg gccgtcata atcccaatc gtccttatc 
      accgttttc gtgtatttt atcgcaacg ttagaagag 
      ttaaaatac gaggaccag tcataccca ggctacatc 
      attgtgtgg gggtgggcc ttgtttggc gtgggtgtg 
      atgcagcct ttgatttgg tgggggatt gaaatcggg 
      aagaattac aaaagggaa ggcgtttcc aaggcaata 
      ctgcggacg ttcaagcat gaaaattgg ggccccaaa 
      gaagaggag ttttacaac aactgggtg gaattcaag 
      cgggaaagg cggcaaaaa acggccacg ggccggcac 
      tggttcaag cacaaatta tgcgtgctt gtaggaaag 
      tag
    mRNA sequence
    • >TC006378 RA
      cgggtgttc cacaaggcc cagttttag ggcccgtgc 
      tatttcaat aaatcagcc aactgcgac cagttgaag 
      aaatggacg aaacgacga aaaacaggg gcaaatggg 
      gcaacagca tcgagttcc taatgtcct gcattgcca 
      tgaacgtgg gcttgggca acatttggc gtttcccct 
      tcgtggcgt acgaaaacg gcggtggcg ccttcctca 
      tcccctaca tctccgtcc taatcctcc tcggccgcc 
      ccatgtact acctggaaa tgtgcctcg gccagttca 
      gcagccgag gcaacgtcg aaatgttcg agtccatag 
      cccaggtct tgaaagggg tcggttttg ggcaactaa 
      tcgggacgt tttccgtgg ccacctact actgctccc 
      tgatggcct tgaccctgt actacttga tccattcgt 
      tccggcccg acctgccct ggtcgcagt gcgacccca 
      actggggtc gggacacgt ggctgggca acaatacgt 
      gcattccga gcaagagca cggaagtgg gggtcgaga 
      attcggtca gctcggctg aagcctggt tcaggatcg 
      aggtgttga gggaagcca cggatttgt cgcatggga 
      tagggtacc ccaattggg agttgacgc tctgcttgc 
      tctgctcgt ggattatca cgttttgta tttgcgcca 
      aaggggtgc aaagctcgg ggaaagcgg cgtattttc 
      ttgcgattt ttccattta ttatacttt tcgcgcttt 
      tgattcggt cggttacgc tggaggggg ctggacaag 
      ggattttgt attttgtca agcccaact gggaaaagt 
      tactaagtg cacaggtat ggtatgcag ccgtcactc 
      aatgtttct tctccttga acatcggtt tcggttcgg 
      ttacaatgt acgcatctt ataacagct tcgatcaca 
      atgtttaca gagacgcaa tggtggtca caactcttg 
      acactttca cttcatttc tatccggtt tgataattt 
      tcgggattt taggaaatt tagcttaca agatgaacg 
      tagaagtgt cagatgttg ttaaatctg gtggaacag 
      gtttagctt ttatctcat accccgaag ctatagctc 
      attttgata ttgtaccat ggttgtttg ctattttgt 
      ttttcttca tgttgtttg tactgggcg ttggaagtt 
      taatagcgt taaaagggt gcgctttta cggtgattt 
      tggacgctt ttccccatt tgaaaacat ggcatgtgt 
      catttggaa cggctttgg ttggctttc tggtcggtt 
      tagtctaca tcaccccgg gtggccaat ttattttca 
      caatggtgg attttttcg gcggtactt tcattttct 
      acgtcctga ccataatcg aagtccttt cggtgatgt 
      ggtggtacg gattggaaa atatctgcc tcgacattg 
      agtttatgt caaaaaaac gtccagggc cgtactggc 
      gaatttgct gggccgtca taatcccaa tcgtcctta 
      tcaccgttt tcgtgtatt ttatcgcaa cgttagaag 
      agttaaaat acgaggacc agtcatacc caggctaca 
      tcattgtgt gggggtggg ccttgtttg gcgtgggtg 
      tgatgcagc ctttgattt ggtggggga ttgaaatcg 
      ggaagaatt acaaaaggg aaggcgttt ccaaggcaa 
      tactgcgga cgttcaagc atgaaaatt ggggcccca 
      aagaagagg agttttaca acaactggg tggaattca 
      agcgggaaa ggcggcaaa aaacggcca cgggccggc 
      actggttca agcacaaat tatgcgtgc ttgtaggaa 
      agtaggcta gggattgaa aatgaatgt caaatgaag 
      cagagaaaa gtaatttgt gacgcctgt gtggttatt 
      agaatgaaa gcattgtta ttgtttgtt tgttttttt 
      tttttaaat aaagtggtc aagaatcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY