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Gene information
General information
TC034835 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7959478..7964060
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034835
    iBeetle screen iB_04123

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034835 RA
      MEKMIAGFL SSPSVLVAI FLGGSVLLL FYILKLFKI 
      EHEEQHQNE PASGPKRDP VAKSKKKPT WNRADKPQN 
      FTNPWLLKT LKSHTGQVL NIDYSASGK YLASCAEED 
      PPEMNPDSN KENLPEEES SPRSKGLSR RQRKNRRPN 
      SPDAKKKFK TEPLRQCLK FNISERTFA DLLHPHLLT 
      YEQLFAMGY PVHSPSHNN SVVFFNYSH FKPPIFDVN 
      AREFVPNQG GYDSHSSSD SSESESSGS EPDYDPAKD 
      QGIERVCVR CGRGFFTTN KEYLTQERC NYHWGKLHT 
      VNALATYSC CKGKPGSVG CATCKLHVW SGVVSGVNG 
      PCEGFVKTR QKKSIPSDG NYGVYALDC EMCYTVAGL 
      EVTKVTVVA MDGRLVYDA YVKPKNEIV DYNTRFSGI 
      TAKDLKPSA TKTLKEVQN DLRGFISAD TILIGHGLE 
      NDLRGLKIV XXXXXXXXX XXXXXXXXX XXXXXXXXX 
      XXFLKREVQ SGPHNSYED ACACMELML YKVRKENKG 
      YKIM
    CDS sequence
    • >TC034835 RA
      atggagaaa atgattgcg ggttttttg agtagtccg 
      agcgtcctt gtcgcgatt ttcctcggc gggagtgtt 
      ttattgctg ttttacatt ttaaaattg ttcaaaatc 
      gagcacgag gagcagcat caaaatgag ccagcgtcg 
      ggccccaag agagatccc gtcgccaaa tccaagaaa 
      aaacccacg tggaatcgc gctgataag ccgcaaaat 
      ttcaccaat ccgtggctg ctcaaaacg ctcaaaagc 
      cacaccgga caagtcctc aacattgat tattcggcc 
      tccgggaaa tatctagcg tcttgtgcc gaagaagac 
      ccccccgag atgaacccc gattcgaac aaggagaat 
      ctccccgag gaggagtcc agcccgcgc agcaagggc 
      ttatcgcgt cgtcagcga aaaaaccgg cgtccaaat 
      tcccccgac gctaagaaa aaattcaaa acggaacct 
      ctgaggcag tgcctcaag ttcaacatc tcggagcgc 
      acattcgcc gacttgtta caccctcac ttgctcacc 
      tacgagcag ctattcgcg atgggctac cctgtacat 
      tcgccatcg cacaacaat tcggtcgtt ttcttcaac 
      tatagccac ttcaaaccg ccgattttc gacgtgaac 
      gcgcgggaa ttcgtcccg aatcagggc ggctatgac 
      agccacagc tcgagcgat tcgagcgaa tctgaaagt 
      tcgggctcg gaacccgac tacgatcct gcgaaggac 
      caaggcatc gaacgtgtg tgtgtgcgg tgcggacgc 
      gggtttttc accaccaat aaggagtac ctcacccag 
      gagcggtgt aactaccac tggggaaag ctacacaca 
      gttaacgca ctcgccacc tactcgtgt tgcaaaggc 
      aagccgggc tcagtgggc tgtgcgacg tgcaaactc 
      cacgtgtgg agcggtgtt gtcagtggg gttaacggc 
      ccgtgcgag ggcttcgtc aaaacgcgg caaaaaaaa 
      tcaatcccg agtgatggc aactatggg gtttacgcc 
      ctcgattgt gagatgtgc tacactgtg gcgggcctg 
      gaggtgacc aaggtcacg gtggtggcc atggacggg 
      cgcttggtg tacgacgct tacgtcaag ccgaaaaac 
      gagattgtc gattacaat acccgattt tcggggatt 
      accgcaaaa gacttgaag ccgagtgcg accaagacg 
      ctcaaggag gtgcagaac gacctgcgg gggttcatc 
      agtgcggac accatcctc atagggcac gggctggag 
      aacgacctc agggggctc aaaattgtg cannnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnttt ctgaagcgc gaggtgcaa agtggcccc 
      cataacagt tacgaggac gcttgcgcc tgcatggaa 
      ctgatgctg tataaagtt aggaaagaa aataaaggt 
      tacaaaatc atgtga
    mRNA sequence
    • >TC034835 RA
      tcgccccca gttttcgtc gagtgaatt tcgccaaaa 
      ctgaatgga tccccgtgt gataatgcc ccatcgtaa 
      catcgaaaa aacgcgcaa aacccgcat gaaattcga 
      aaaggcccg ccagaccat taccaaccc gaagcgcat 
      gcgtcccca aatccccta cacgaggag tcagtagcg 
      cgctggatt ttgccgaaa acgaaatgg agaaaatga 
      ttgcgggtt ttttgagta gtccgagcg tccttgtcg 
      cgattttcc tcggcggga gtgttttat tgctgtttt 
      acattttaa aattgttca aaatcgagc acgaggagc 
      agcatcaaa atgagccag cgtcgggcc ccaagagag 
      atcccgtcg ccaaatcca agaaaaaac ccacgtgga 
      atcgcgctg ataagccgc aaaatttca ccaatccgt 
      ggctgctca aaacgctca aaagccaca ccggacaag 
      tcctcaaca ttgattatt cggcctccg ggaaatatc 
      tagcgtctt gtgccgaag aagaccccc ccgagatga 
      accccgatt cgaacaagg agaatctcc ccgaggagg 
      agtccagcc cgcgcagca agggcttat cgcgtcgtc 
      agcgaaaaa accggcgtc caaattccc ccgacgcta 
      agaaaaaat tcaaaacgg aacctctga ggcagtgcc 
      tcaagttca acatctcgg agcgcacat tcgccgact 
      tgttacacc ctcacttgc tcacctacg agcagctat 
      tcgcgatgg gctaccctg tacattcgc catcgcaca 
      acaattcgg tcgttttct tcaactata gccacttca 
      aaccgccga ttttcgacg tgaacgcgc gggaattcg 
      tcccgaatc agggcggct atgacagcc acagctcga 
      gcgattcga gcgaatctg aaagttcgg gctcggaac 
      ccgactacg atcctgcga aggaccaag gcatcgaac 
      gtgtgtgtg tgcggtgcg gacgcgggt ttttcacca 
      ccaataagg agtacctca cccaggagc ggtgtaact 
      accactggg gaaagctac acacagtta acgcactcg 
      ccacctact cgtgttgca aaggcaagc cgggctcag 
      tgggctgtg cgacgtgca aactccacg tgtggagcg 
      gtgttgtca gtggggtta acggcccgt gcgagggct 
      tcgtcaaaa cgcggcaaa aaaaatcaa tcccgagtg 
      atggcaact atggggttt acgccctcg attgtgaga 
      tgtgctaca ctgtggcgg gcctggagg tgaccaagg 
      tcacggtgg tggccatgg acgggcgct tggtgtacg 
      acgcttacg tcaagccga aaaacgaga ttgtcgatt 
      acaataccc gattttcgg ggattaccg caaaagact 
      tgaagccga gtgcgacca agacgctca aggaggtgc 
      agaacgacc tgcgggggt tcatcagtg cggacacca 
      tcctcatag ggcacgggc tggagaacg acctcaggg 
      ggctcaaaa ttgtgcann nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nntttctga 
      agcgcgagg tgcaaagtg gcccccata acagttacg 
      aggacgctt gcgcctgca tggaactga tgctgtata 
      aagttagga aagaaaata aaggttaca aaatcatgt 
      gacatgtta tcatgcttt atttattaa acgcgtcat 
      ttgtcatta ctatgtgat atcgtgttc ctagtgaat 
      ctcattacg tgatattta cgcaggaat caatgcaag 
      tttttatgt tttttgatt actttaagg acgtaatta 
      gtgtacaaa gtggtcaag actaaagtt cctagcaac 
      ttttttaca ttttgtacg gactgtaga ttcagccag 
      tttgccact cgtttatgt gaaatagta ttaccctag 
      acttaagtc acttgtgtt catttgttt gttatttgt 
      ttaaattag gcaaaaagg ctgtgctga aacgtgata 
      cagttgtat ctattttta ataaaccgt tttttaatt 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY