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Gene information
General information
TC006359 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7877777..7883679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006359
    iBeetle screen iB_04121

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles structure shortened - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006359 RA
      MGNVDTKLN FRKAVVQLT SKSEPIDAN DDSFWEQFW 
      SENVTNVQD IFTLVPAAE IRALREDAP SNLATLCYK 
      AVEKLVKAV DSSCRTQHE QQTVLNCAR LLTRILPYI 
      FEDPEWRGF FWSSLPSQG EDEDESVPL AQSLLNAVC 
      DLLFCPDFT VVASRKSGP DKAEELSAI DSCEYIWEA 
      GVGFAHSPP RSPVLDSNR TELLKLLLT CFSETMYQP 
      PTDISQNPN KWIAHLTSS ENRHALPMF TSLLNTVCA 
      YDPVGLGVP YNHLLFNDS LEPLVEAAL QILIVTLDH 
      DTSSSSPAE GEDSTVPDN LFINYLSRI HRDEDFEFI 
      LTGVTRLLN NPLVQTYLP NSTKKVHFH QELLVFFWK 
      MCDYNKKFL YFVLKSSDV LEVLVPILY HLNDSRADQ 
      SRVGLMHIG VFILLLLSG ERNFGVRLN KPYTATIPM 
      DIPVFTGTH ADLLIIVFH KIITTGHQR LQPLFDCLL 
      TILVNVSPY LKTLSMVAS TKLLHLLEA FSTPWFLFS 
      SSTNHHLVF FLLEIFNNI IQYQFDGNS NLVYTIIRK 
      RQVFHNLAN LPSDYGTIA KSLNKRTRS RMSSTTSHE 
      NVAELAMEG SHPALPAEP GTLKATLPD TPGIGQMTE 
      KEPAHPSYQ NEQLLTNGM ASITMNTPT ASTPEEPTN 
      DNSEQKLSR SPSRQSVAT RNSLRVSAS GDKNPAHWT 
      PTGEWVHSW KSKLPLQTI MRLLQVLVP QVEKICIDK 
      GLTDESEIL KFLQHGTLV GLLPVPHPI LIRKYQANS 
      GTTTWFRTY MWGVIYLRN VDPPVWYDT DVKLFEIQR 
      I
    CDS sequence
    • >TC006359 RA
      atgggtaac gtcgacacg aaattgaat ttccgcaaa 
      gcggtggtg caactgacg tccaaatca gagcccatt 
      gatgcgaat gacgactcg ttttgggag caattctgg 
      tccgagaac gttacaaac gtccaagac atattcact 
      ctagtgcct gcggccgaa atccgggcg ctccgcgaa 
      gacgcgccg tcgaatctc gccacctta tgctacaaa 
      gcggtggag aagttagtc aaggctgtt gatagtagt 
      tgtaggacg cagcacgag caacaaacc gtattgaat 
      tgtgcgcgc ttgctgacg cggatcttg ccgtatatt 
      tttgaggac cccgagtgg cggggattt ttctggtcg 
      tcgctgccc agccaaggg gaggacgag gatgagtcg 
      gtgccgttg gcacagtcg ttgctaaac gccgtttgt 
      gacttgcta ttctgcccg gattttacg gtggtagcg 
      agccgaaaa agtggccct gataaagcc gaggaattg 
      tcggcgatt gatagctgc gagtacata tgggaggcc 
      ggggtcggg tttgcgcac tcgccccca cgcagcccc 
      gtgcttgac tccaaccga actgagcta cttaaacta 
      ctgttgact tgctttagc gagactatg tatcagccc 
      ccgaccgac atttcgcaa aatcccaac aaatggata 
      gctcatttg acttcgtct gaaaaccgg catgctcta 
      cccatgttt acctcgctt ttgaataca gtctgtgct 
      tatgacccc gtgggcttg ggggtgcct tacaaccac 
      ctacttttc aatgattcg cttgagcca ttggttgaa 
      gccgctttg cagattttg attgtgacg ctggatcac 
      gatacgagt tcgtcaagt ccggcagag ggcgaagat 
      agtacggtc cccgataat ctctttatt aattacctc 
      tcgcggata caccgcgac gaagacttc gaatttatt 
      cttaccggt gttacaaga ttgttgaat aacccttta 
      gtacagacg tacttgccg aattcgacc aagaaagtg 
      cactttcat caggagttg ttggtgttt ttttggaaa 
      atgtgcgat tataacaag aagtttttg tattttgtg 
      ttgaaaagt agcgacgtt ttggaagta ctggtgcct 
      attctctat catctcaac gattcgaga gccgatcag 
      tctagggta ggcctgatg cacatagga gtatttatt 
      ttgctttta ttgagcgga gagcgaaat ttcggcgtt 
      cgcctcaac aaaccttac accgcaaca attccaatg 
      gacattccc gtctttacc ggcacacat gccgacctt 
      ctcatcata gtttttcac aaaataata accaccggc 
      caccaaaga cttcaacca ctattcgat tgccttctc 
      acaattcta gtcaatgta tctccatac ttgaaaacc 
      ctctcaatg gtcgcaagc acgaagctt ttgcacctt 
      ttagaagct ttcagtaca ccttggttc ctcttttcc 
      tcctcgacc aatcaccac ctagtcttt ttccttctg 
      gaaatcttc aacaacata atccagtac caattcgac 
      ggtaactcg aacctggtg tataccata atccggaaa 
      cgccaagtg ttccacaat ctggcaaac ctgccaagt 
      gattatggc accattgca aaatcgcta aacaaacga 
      acgcgaagt cgcatgtcg tcgacgacg agtcacgaa 
      aacgtggcc gagttggcc atggaaggg tcgcatccg 
      gcacttccg gcagaaccc ggaacgttg aaagccaca 
      cttccggac acgcccgga atcggccaa atgaccgaa 
      aaggaaccg gcacacccc tcgtaccaa aacgaacaa 
      ttgttgacc aatggcatg gcgtcgatt actatgaac 
      acgcccacg gcgagcacg cccgaagaa ccgaccaat 
      gataacagt gagcagaag ttgagtcgc agtccgtcg 
      cgccaatcc gtggccacg agaaacagt ttgcgggtc 
      tccgcctcc ggggataag aaccccgcc cattggacg 
      cccaccggc gaatgggtg cactcgtgg aagtccaaa 
      ctgcccctc cagaccatc atgcggcta ttgcaggtc 
      ttggtgccg caagttgag aaaatttgc atcgataaa 
      ggcctaact gacgaaagc gaaattttg aaattcctg 
      caacatgga actttggtg gggctgtta ccggtgccg 
      caccctatt ctcatacga aagtatcag gcgaattcg 
      gggacgacc acctggttt aggacgtac atgtggggc 
      gtgatttat ttgaggaat gtcgatccg ccggtttgg 
      tacgacacc gacgtaaaa ctatttgag attcagcga 
      atctaa
    mRNA sequence
    • >TC006359 RA
      tatcaataa acgcgaaat tatcgcgtg tcgttgata 
      acagttggc gttacgtca ctgtaaata gcccccacg 
      tcgcggcaa accccacta cggcccctc gggtcattg 
      acgtcgaac ctgtcaaag tgacgtttc gtaatgtca 
      gtccaaatt gtcaaactt taacttaaa ttcgaaaaa 
      tatgggtaa cgtcgacac gaaattgaa tttccgcaa 
      agcggtggt gcaactgac gtccaaatc agagcccat 
      tgatgcgaa tgacgactc gttttggga gcaattctg 
      gtccgagaa cgttacaaa cgtccaaga catattcac 
      tctagtgcc tgcggccga aatccgggc gctccgcga 
      agacgcgcc gtcgaatct cgccacctt atgctacaa 
      agcggtgga gaagttagt caaggctgt tgatagtag 
      ttgtaggac gcagcacga gcaacaaac cgtattgaa 
      ttgtgcgcg cttgctgac gcggatctt gccgtatat 
      ttttgagga ccccgagtg gcggggatt tttctggtc 
      gtcgctgcc cagccaagg ggaggacga ggatgagtc 
      ggtgccgtt ggcacagtc gttgctaaa cgccgtttg 
      tgacttgct attctgccc ggattttac ggtggtagc 
      gagccgaaa aagtggccc tgataaagc cgaggaatt 
      gtcggcgat tgatagctg cgagtacat atgggaggc 
      cggggtcgg gtttgcgca ctcgccccc acgcagccc 
      cgtgcttga ctccaaccg aactgagct acttaaact 
      actgttgac ttgctttag cgagactat gtatcagcc 
      cccgaccga catttcgca aaatcccaa caaatggat 
      agctcattt gacttcgtc tgaaaaccg gcatgctct 
      acccatgtt tacctcgct tttgaatac agtctgtgc 
      ttatgaccc cgtgggctt gggggtgcc ttacaacca 
      cctactttt caatgattc gcttgagcc attggttga 
      agccgcttt gcagatttt gattgtgac gctggatca 
      cgatacgag ttcgtcaag tccggcaga gggcgaaga 
      tagtacggt ccccgataa tctctttat taattacct 
      ctcgcggat acaccgcga cgaagactt cgaatttat 
      tcttaccgg tgttacaag attgttgaa taacccttt 
      agtacagac gtacttgcc gaattcgac caagaaagt 
      gcactttca tcaggagtt gttggtgtt tttttggaa 
      aatgtgcga ttataacaa gaagttttt gtattttgt 
      gttgaaaag tagcgacgt tttggaagt actggtgcc 
      tattctcta tcatctcaa cgattcgag agccgatca 
      gtctagggt aggcctgat gcacatagg agtatttat 
      tttgctttt attgagcgg agagcgaaa tttcggcgt 
      tcgcctcaa caaacctta caccgcaac aattccaat 
      ggacattcc cgtctttac cggcacaca tgccgacct 
      tctcatcat agtttttca caaaataat aaccaccgg 
      ccaccaaag acttcaacc actattcga ttgccttct 
      cacaattct agtcaatgt atctccata cttgaaaac 
      cctctcaat ggtcgcaag cacgaagct tttgcacct 
      tttagaagc tttcagtac accttggtt cctcttttc 
      ctcctcgac caatcacca cctagtctt tttccttct 
      ggaaatctt caacaacat aatccagta ccaattcga 
      cggtaactc gaacctggt gtataccat aatccggaa 
      acgccaagt gttccacaa tctggcaaa cctgccaag 
      tgattatgg caccattgc aaaatcgct aaacaaacg 
      aacgcgaag tcgcatgtc gtcgacgac gagtcacga 
      aaacgtggc cgagttggc catggaagg gtcgcatcc 
      ggcacttcc ggcagaacc cggaacgtt gaaagccac 
      acttccgga cacgcccgg aatcggcca aatgaccga 
      aaaggaacc ggcacaccc ctcgtacca aaacgaaca 
      attgttgac caatggcat ggcgtcgat tactatgaa 
      cacgcccac ggcgagcac gcccgaaga accgaccaa 
      tgataacag tgagcagaa gttgagtcg cagtccgtc 
      gcgccaatc cgtggccac gagaaacag tttgcgggt 
      ctccgcctc cggggataa gaaccccgc ccattggac 
      gcccaccgg cgaatgggt gcactcgtg gaagtccaa 
      actgcccct ccagaccat catgcggct attgcaggt 
      cttggtgcc gcaagttga gaaaatttg catcgataa 
      aggcctaac tgacgaaag cgaaatttt gaaattcct 
      gcaacatgg aactttggt ggggctgtt accggtgcc 
      gcaccctat tctcatacg aaagtatca ggcgaattc 
      ggggacgac cacctggtt taggacgta catgtgggg 
      cgtgattta tttgaggaa tgtcgatcc gccggtttg 
      gtacgacac cgacgtaaa actatttga gattcagcg 
      aatctaaac gtaatcaaa atattacct aatttattt 
      ttttattta tttagatcg atttttcca tatttatgt 
      aaatgtgca tttaatatt aagactgtt tacaaatgg 
      cttttaaat gtactttta atgtagtta ccgttgttt 
      atcttgtga tcagttcaa tttttaagt ggttggtat 
      cacctgggc aaggctggg aaatatacg ttggaaata 
      actttatat gtcgcagtt ttgaccgac tttacacca 
      agatcactt attttaatc ctttactgt tgaagcttt 
      tgtaacaag aaaaaaaat tgaagctat ttgtgtttc 
      gtttttgta gaaatttat tttcaaatg tatacgttt 
      gttacagtg tataacgga ttaaataaa taaatgact 
      atattcaaa cctggggat ttgtatttt gagggttgg 
      taaaaaacg attgtaggt acgctcttt attttattt 
      tacatagct gactagttc atacgtaat attttacaa 
      aaaaaatct acagttatt aaggattgc atttgacgc 
      agtctcaaa tacaataac tagaaaaat gatgcaata 
      aagaacaca aaaagaaat ggcctctgc atacgttga 
      aaacttcat taaaaatct aatcatgtc taattaaaa 
      agtagattt ttctgatat tttttgata tgtttacat 
      gaccatgga aattccgca attcggtgg ccactttaa 
      aatacacag tcaccgaaa caacagttt acgtcctag 
      atttgaaat ttatattag gtcggtttt tgataaatg 
      ttagaaaaa atataataa cattacgga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY