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Gene information
General information
TC006353 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7771637..7785587
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006353
    iBeetle screen iB_04119

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006353 RA
      MAKADLLRS LMDVVGFLV LCLVVIIKS IVKSILPAS 
      YQHYKQLKG EIILITGGG GGLGRLLAL RLARLDAIV 
      VLWDVNVKA VEETVELVK GVGGVAYGF KCDLADKED 
      VYKIANTTR QEVGEVTIL INNAGIVSG NLLLNTPDH 
      LIKRTFDVN ILAHFWTVK AFLPQMIAK NHGHIVTIA 
      SMAGFVGIN KLVDYCSSK HAAVGFDEA LRVELEAQG 
      VDGVKTTVI CPYFIQSTG MFDNVNSRF LPTLKSNDV 
      ADRIVEGLR REEIVVMIP GIFKTGVIF KFLFPWACI 
      SMFLRGLVP DAVPHHHLP VQTSEKSDF TTKSDIMKS 
      SLASLDQRE GISGKNL
    CDS sequence
    • >TC006353 RA
      atggccaag gccgatttg ttaaggtcc ttaatggac 
      gtggtggga tttctggtg ctgtgttta gtcgttatc 
      attaaatca atcgtaaaa tcgattctc cccgcctct 
      tatcaacac tacaagcaa ctcaaaggg gagattatc 
      cttatcaca ggaggcggt ggagggctg gggaggtta 
      ctagccctc aggttggca cgactggac gccatcgtc 
      gtgctatgg gacgtcaac gttaaagcc gttgaagaa 
      actgtggaa ttagtaaaa ggcgtagga ggcgtcgct 
      tatggcttc aagtgcgac cttgcggac aaagaagac 
      gtgtacaaa atagcaaac acaacacgt caagaagtt 
      ggcgaagtg accatctta ataaacaac gccggaatt 
      gtttccgga aacctcctc ctgaacaca ccggaccac 
      ctaatcaag cggacgttc gacgtcaac attttggcc 
      cacttctgg accgttaag gcgtttttg ccccaaatg 
      atcgccaaa aaccacggc cacatcgtc acaatcgcg 
      tccatggcc ggcttcgtc ggaatcaac aaactggtc 
      gattactgc tcctccaaa cacgccgca gttggcttt 
      gacgaggcc cttagggtt gagctggaa gcccaaggg 
      gttgatggg gtcaaaacc acggtcatt tgcccgtat 
      tttatccaa tcaaccggg atgttcgac aatgtcaac 
      tcccgtttt ctgcccact ttgaagtcc aacgacgtg 
      gcggatcga attgttgaa gggctgcgc agggaagag 
      atcgtcgtg atgatccct gggattttc aaaactggc 
      gttattttc aaatttttg ttcccatgg gcgtgcatt 
      tcgatgttt ttgagaggt ttggtcccg gatgccgtc 
      ccccatcac caccttcca gtgcaaact agtgagaaa 
      agtgatttt acgaccaag tcggatatt atgaagagt 
      tctttagct tcgctagac cagcgagag gggattagt 
      gggaagaat ctatag
    mRNA sequence
    • >TC006353 RA
      cccattgac caccacgcc aaatacttt tgtttacag 
      ccgccggat taccagttt ctaaactct agtgaacgt 
      gaaaccacc gtgaaaacc tagttcttc acatcagtc 
      aactgagca ttttttgat ttttattat ggactacta 
      accggcact ccagatctt tgcctggcc ttgaaaatg 
      ctacaaatc tagtcaaac gtgccttaa ggatggcca 
      aggccgatt tgttaaggt ccttaatgg acgtggtgg 
      gatttctgg tgctgtgtt tagtcgtta tcattaaat 
      caatcgtaa aatcgattc tccccgcct cttatcaac 
      actacaagc aactcaaag gggagatta tccttatca 
      caggaggcg gtggagggc tggggaggt tactagccc 
      tcaggttgg cacgactgg acgccatcg tcgtgctat 
      gggacgtca acgttaaag ccgttgaag aaactgtgg 
      aattagtaa aaggcgtag gaggcgtcg cttatggct 
      tcaagtgcg accttgcgg acaaagaag acgtgtaca 
      aaatagcaa acacaacac gtcaagaag ttggcgaag 
      tgaccatct taataaaca acgccggaa ttgtttccg 
      gaaacctcc tcctgaaca caccggacc acctaatca 
      agcggacgt tcgacgtca acattttgg cccacttct 
      ggaccgtta aggcgtttt tgccccaaa tgatcgcca 
      aaaaccacg gccacatcg tcacaatcg cgtccatgg 
      ccggcttcg tcggaatca acaaactgg tcgattact 
      gctcctcca aacacgccg cagttggct ttgacgagg 
      cccttaggg ttgagctgg aagcccaag gggttgatg 
      gggtcaaaa ccacggtca tttgcccgt attttatcc 
      aatcaaccg ggatgttcg acaatgtca actcccgtt 
      ttctgccca ctttgaagt ccaacgacg tggcggatc 
      gaattgttg aagggctgc gcagggaag agatcgtcg 
      tgatgatcc ctgggattt tcaaaactg gcgttattt 
      tcaaatttt tgttcccat gggcgtgca tttcgatgt 
      ttttgagag gtttggtcc cggatgccg tcccccatc 
      accaccttc cagtgcaaa ctagtgaga aaagtgatt 
      ttacgacca agtcggata ttatgaaga gttctttag 
      cttcgctag accagcgag aggggatta gtgggaaga 
      atctatagt gattttttt tctgtgaaa ggtttaatt 
      aagtacaga cagtgggtt ttattgttt agttttaaa 
      actgtgtga cttaattta attaaatat ttttattac 
      taaacctgt ctctttttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY