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Gene information
General information
TC006332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7321191..7324921
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006332
    iBeetle screen iB_04114

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva cuticle partially unilateralnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006332 RA
      MEARKPVLN QKNEAVIRV KSAVPKNRQ DLLKWNGWG 
      YRDSKFIFD SAANEVRFT GQRYPIGDL RLPHLMGWV 
      KENFDVDFS KPKKAQEVP PEEKFPKPQ ISPENLARI 
      TNLDISYSL KGLDRLIRA HGQTLHDIY TLRTSFFER 
      IPDIVVWPT CHSDVVKLV RLADESDMV VIPFGGGTA 
      VSGAVECPT GESRTIISL DTSQMNRIL WIDRENLVA 
      CCESGIIGQ DLERELRKL GFTSGHEPD SYEFSSLGG 
      WVATRASGM KKNLYGNIE DLLVHVKMV TPKGVLEKN 
      CQVPRLSCG PDFNHVIMG SEGSLGVIT EVVIKVRPL 
      PKCHRFGSI VFPDFEAGL ACMREVARQ RCQPSSIRL 
      MDNGQFKFG MILKPESSY LGLILDGLK KFYVTKIKG 
      FNVDTMCVM TLLFEGEEA DVKAQEKKI YSIGLQFGG 
      MPAGQTNGE RGYMLTFVI AYIRDLALE YEIVAESFE 
      TSVPWDRAL VLCKNVKEV IAQECRSQG IKYYMINCR 
      VTQTYDAGC VIYFYLGFN HRDVENPVT VHEILETKA 
      RDEIIACGG SISHHHGVG KLRRRWYAG TVSQVGVDL 
      YLATKKELD PKNIFATNN LIKAKL
    CDS sequence
    • >TC006332 RA
      atggaagca cgaaaaccg gtacttaac caaaaaaac 
      gaagcggtt attcgagtg aagagtgcc gtgccgaaa 
      aaccgccaa gacttgcta aaatggaac gggtggggg 
      taccgagac tcgaaattc attttcgac agtgctgcc 
      aacgaggtg cgctttacg gggcaaaga tatcccatc 
      ggtgacctc cgcttgccc catttgatg ggctgggtg 
      aaggaaaac ttcgatgtt gatttttcc aaacctaag 
      aaagcgcag gaagtccct ccagaggag aaattcccc 
      aaaccgcaa atatcccca gagaatctc gccagaata 
      accaacctg gacataagt tactccctg aagggccta 
      gaccggctc attcgggcc cacggacag accctacat 
      gacatatac acccttcga acgtcgttt ttcgaacgt 
      atccccgac attgtggtg tggccaacc tgtcacagt 
      gacgttgtc aagttggta cgactggct gatgagtcg 
      gacatggtc gttataccg ttcgggggc gggactgcg 
      gtttcgggc gccgtcgag tgtcccacc ggggaatcc 
      cgaaccatt atctcccta gatacgtcc caaatgaac 
      cggatttta tggatagat cgcgagaat ctcgtcgct 
      tgttgcgag agcgggatc ataggccaa gacctggaa 
      cgtgaactc cggaaactg ggcttcact tccgggcac 
      gagcccgac agctacgag ttttcgagt ctaggaggc 
      tgggtggct acgagggcg tccggaatg aagaaaaat 
      ttgtatggc aacattgag gatctccta gtgcatgtc 
      aaaatggtg acgccgaaa ggcgtcctt gagaagaac 
      tgtcaagtc cctcgtttg agttgcggg ccggacttt 
      aaccacgtg attatgggt tcggaaggc tcgctcggg 
      gtaatcacc gaagtggtg atcaaagtc cgaccattg 
      ccgaagtgc catcggttc ggttcgatc gttttcccc 
      gattttgag gcggggctt gcgtgtatg cgtgaagtt 
      gccaggcaa cggtgccag ccatcttcg attcggttg 
      atggacaat ggacagttt aaattcggg atgattttg 
      aagccggaa tcgagttac cttggcttg attttggac 
      ggtttgaag aagttttat gttacaaaa attaagggt 
      tttaatgtc gatacgatg tgtgtcatg actttgctc 
      tttgagggg gaggaagcg gacgtgaag gcgcaggag 
      aaaaaaata tatagtata gggttgcag tttgggggc 
      atgccggct gggcagacc aacggggag cgggggtac 
      atgctcact ttcgtcata gcctacatc agggacctg 
      gctttggag tacgagatc gtggcggag tctttcgag 
      acttcagtc ccgtgggac cgcgcccta gtcctgtgt 
      aaaaacgtg aaagaggtc atagcccag gaatgccga 
      tcccaaggg attaaatat tacatgata aactgccga 
      gtgacccaa acctacgac gccggttgt gtcatctac 
      ttctatctc ggtttcaat caccgtgat gttgagaat 
      cccgtcaca gttcacgaa atccttgaa acaaaagcc 
      agggatgaa attatagct tgtggtggg agtatttcg 
      caccatcac ggagttgga aaactaaga aggcggtgg 
      tatgcagga acggtgtca caagtaggc gtcgacttg 
      tatttagcc acgaagaag gagttggat ccgaagaat 
      atcttcgcg acgaataat ttaattaag gccaaattg 
      tga
    mRNA sequence
    • >TC006332 RA
      cgcgtcacg ccttgaggg ggctgttaa ggcggtgcg 
      atactcact ttaccttca atttaattg gtttttcat 
      ggtacctat aaaaggcaa aacccagaa agttggagt 
      attttttcg taaggcatg gaagcacga aaaccggta 
      cttaaccaa aaaaacgaa gcggttatt cgagtgaag 
      agtgccgtg ccgaaaaac cgccaagac ttgctaaaa 
      tggaacggg tgggggtac cgagactcg aaattcatt 
      ttcgacagt gctgccaac gaggtgcgc tttacgggg 
      caaagatat cccatcggt gacctccgc ttgccccat 
      ttgatgggc tgggtgaag gaaaacttc gatgttgat 
      ttttccaaa cctaagaaa gcgcaggaa gtccctcca 
      gaggagaaa ttccccaaa ccgcaaata tccccagag 
      aatctcgcc agaataacc aacctggac ataagttac 
      tccctgaag ggcctagac cggctcatt cgggcccac 
      ggacagacc ctacatgac atatacacc cttcgaacg 
      tcgtttttc gaacgtatc cccgacatt gtggtgtgg 
      ccaacctgt cacagtgac gttgtcaag ttggtacga 
      ctggctgat gagtcggac atggtcgtt ataccgttc 
      gggggcggg actgcggtt tcgggcgcc gtcgagtgt 
      cccaccggg gaatcccga accattatc tccctagat 
      acgtcccaa atgaaccgg attttatgg atagatcgc 
      gagaatctc gtcgcttgt tgcgagagc gggatcata 
      ggccaagac ctggaacgt gaactccgg aaactgggc 
      ttcacttcc gggcacgag cccgacagc tacgagttt 
      tcgagtcta ggaggctgg gtggctacg agggcgtcc 
      ggaatgaag aaaaatttg tatggcaac attgaggat 
      ctcctagtg catgtcaaa atggtgacg ccgaaaggc 
      gtccttgag aagaactgt caagtccct cgtttgagt 
      tgcgggccg gactttaac cacgtgatt atgggttcg 
      gaaggctcg ctcggggta atcaccgaa gtggtgatc 
      aaagtccga ccattgccg aagtgccat cggttcggt 
      tcgatcgtt ttccccgat tttgaggcg gggcttgcg 
      tgtatgcgt gaagttgcc aggcaacgg tgccagcca 
      tcttcgatt cggttgatg gacaatgga cagtttaaa 
      ttcgggatg attttgaag ccggaatcg agttacctt 
      ggcttgatt ttggacggt ttgaagaag ttttatgtt 
      acaaaaatt aagggtttt aatgtcgat acgatgtgt 
      gtcatgact ttgctcttt gagggggag gaagcggac 
      gtgaaggcg caggagaaa aaaatatat agtataggg 
      ttgcagttt gggggcatg ccggctggg cagaccaac 
      ggggagcgg gggtacatg ctcactttc gtcatagcc 
      tacatcagg gacctggct ttggagtac gagatcgtg 
      gcggagtct ttcgagact tcagtcccg tgggaccgc 
      gccctagtc ctgtgtaaa aacgtgaaa gaggtcata 
      gcccaggaa tgccgatcc caagggatt aaatattac 
      atgataaac tgccgagtg acccaaacc tacgacgcc 
      ggttgtgtc atctacttc tatctcggt ttcaatcac 
      cgtgatgtt gagaatccc gtcacagtt cacgaaatc 
      cttgaaaca aaagccagg gatgaaatt atagcttgt 
      ggtgggagt atttcgcac catcacgga gttggaaaa 
      ctaagaagg cggtggtat gcaggaacg gtgtcacaa 
      gtaggcgtc gacttgtat ttagccacg aagaaggag 
      ttggatccg aagaatatc ttcgcgacg aataattta 
      attaaggcc aaattgtga agttacctt tgtattttt 
      gtattactg tgatatgga gtggcattg agcgtgttt 
      cgtttgttt gtttttttt taataataa atgtgtgaa 
      attgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY