Status
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Gene information
General information
TC034042
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006328,TC006327
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7238071..7242191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034042
    iBeetle screen iB_04113

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    longitudinal dorsal thoracic muscles mostly structure not present - Number of animals showing the phenotype: 10

    oblique dorsal thoracic muscles distal mostlystructure shortened - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034042 RA
      MTNSPGPST STTVKTTDP DLKKKKRTR RKPRTRSKK 
      DTDSPPDVD LLPLLPTFH IQPVGSPHA TVYKLRDQS 
      QGPINLQSL LKCSLFHTR VPSCDPNSA LNLLQKISN 
      LAVNGIREA SSSKKKKGK RKERVFFPE YLSEAEVES 
      GLETRELMK GYIRINPKN AKESYVGNE DVSKTDYMI 
      TSVLDRNRA FDGDEVVLR LKTETEDKK KTMEVVYIT 
      KKIHPRTAV GFLAPIEKN KEYALFTPR DKRIPKIKI 
      PRISWPSGF QSKPQLYQD ILFIVKITD WKDVKYAIG 
      TITENLGLS GDLKIESLA ILREFCLDP TPFGDDIRP 
      FLPKSTKIS TSDLKNRED LRKECVFTI DPITARDLD 
      DALSCKELP NGNYEVGVH ISDASFFLP ENTRLDEIV 
      KSKATTIYM VDSVYHMLP VELCFHCSL LPGEDKLAF 
      SVFWELTPT GDIVDFRIK RSVINSCVQ LAYEHAQVM 
      IDDPDRKFE RDELPEIYN GFNAHDLSK RVNDLQRIA 
      VCLREKRRQ NGSLKINQI KIGFVLDPA TGEPKEFYI 
      CENKPAHRL IEEFMLLAN MTVAKWINE AYPDLAFLR 
      LHEAPKPTM MTELQKSLE TLGLHIDIS TSGGIQASL 
      NKYISEDDF GKARMIVLN HLLAKPMKR ARYFCAGAL 
      EEELDYSHY ALSIPIYTH FTSPIRRYA DIMVHRLLA 
      ASLNYREKP TWAPEEVAT IAETCNRQK YHAKRAGEA 
      SSDLFLAHF VEKNQPVIE NAVVVDAKE RSIDVIVIR 
      TGSVVRIYT NNFGDKVTW KSEEIKVRK SSSDEENAK 
      KMWKIFVKF PRTVEEPEV CIVLEIFTL VRVELARKH 
      KTNKLDAKL LRPIQLV
    CDS sequence
    • >TC034042 RA
      atgacaaat tccccggga ccaagcacc tcaacgacc 
      gtcaagaca accgaccct gaccttaag aagaaaaaa 
      cgcacgcgt cggaagccc cgcacccgc tccaagaaa 
      gacactgac tcgcccccc gacgtcgac ctgctgcct 
      ctactgcca actttccac attcaaccg gtcggcagc 
      ccccatgcc actgtctac aaactgcga gaccagtcc 
      caaggcccc atcaactta cagtctttg ctcaagtgc 
      agtttattt cacacgaga gtgccaagc tgtgacccc 
      aactcggcc ctaaacctc ttacagaaa atttccaat 
      ttggctgtt aatggaatt cgcgaagct agttcctcg 
      aaaaagaag aaagggaag cggaaagaa cgtgttttt 
      ttcccggag tatttaagc gaggcggag gtcgagtcc 
      gggctggaa acaagggag ttgatgaag ggttatatt 
      aggattaac cccaaaaat gcgaaagaa agttatgtg 
      ggaaatgag gatgtttcc aagacggat tatatgatt 
      acgtccgtt ttggaccgg aaccgggcc tttgacggc 
      gacgaggtc gttttgagg ctcaaaaca gaaacggaa 
      gataagaaa aagaccatg gaggttgtt tacatcaca 
      aagaagatt cacccccgg accgctgtt ggtttcctt 
      gcccccatt gaaaagaat aaagagtac gccctgttc 
      accccacgc gacaaacgc atcccgaaa atcaaaatt 
      ccgagaata agttggcca agtggtttc caatccaag 
      ccccaacta taccaagac attcttttc atcgttaaa 
      ataacggat tggaaagac gtaaagtat gccataggc 
      acgattacc gagaatttg ggtttgagt ggtgatctc 
      aaaatcgag tctttggcg attttgcgc gaattttgt 
      ctcgatccg actccgttc ggtgacgat atcaggcca 
      ttcctgcca aaaagtacg aaaatttca acaagtgac 
      ttaaaaaat cgtgaggat ttacgcaaa gagtgtgtt 
      tttacgatt gatccaatc acagcacgc gatttggac 
      gatgctttg tcgtgcaag gagttgcca aatggaaat 
      tacgaagtt ggcgttcac atttccgat gcttcgttt 
      ttccttccg gaaaacacg cgactggac gaaattgtg 
      aaaagcaaa gctactacg atctacatg gttgacagt 
      gtgtatcac atgcttccg gtggagttg tgcttccat 
      tgttcgcta cttccgggt gaagataaa ctcgcattt 
      agtgttttt tgggagttg acaccgact ggtgatata 
      gtcgatttt cggatcaag cgatcagtg atcaattca 
      tgtgttcaa ctagcctac gagcatgcg caagtcatg 
      attgatgat ccggaccgg aagtttgaa cgagacgag 
      cttccggag atttacaac gggtttaat gcacatgat 
      ctcagtaag cgggtcaat gatttgcaa aggattgcc 
      gtttgtttg agggagaaa cggaggcaa aatgggtcg 
      ttgaaaatt aatcaaatt aaaattggt ttcgtgctg 
      gatccagca accggagaa ccgaaagag ttttacatt 
      tgtgaaaat aaaccagcg catagatta atagaggag 
      tttatgctt ttggccaac atgaccgtg gccaagtgg 
      ataaatgag gcctacccg gacctggcc tttctgaga 
      cttcacgag gcgcccaag ccgaccatg atgaccgag 
      ctccaaaaa agcctcgaa accctgggc ctccacatc 
      gacatctcc acttcgggg ggcatccaa gcctccttg 
      aacaagtac atcagtgag gacgatttt ggcaaagcc 
      cgcatgatc gtcctcaac cacctcctg gccaagcct 
      atgaaaagg gcgcgatat ttttgtgcc ggtgccctt 
      gaggaggag ctcgattac agtcattac gccctcagt 
      atacccatc tacacccac tttacgtca ccaatccgt 
      cgatatgct gacataatg gtgcacaga ctgctcgca 
      gcgagttta aactatcga gagaaaccc acttgggcg 
      cctgaggag gtggcaacg attgccgaa acgtgtaac 
      cggcagaaa tatcacgcc aagcgggcc ggggaggct 
      agtagtgac ctgtttttg gcccatttt gtcgaaaag 
      aatcaacca gtgattgaa aatgctgtg gttgtggat 
      gcgaaagaa aggtctatt gatgttatt gtgattagg 
      acgggaagt gttgtcagg atttataca aataatttt 
      ggggataaa gttacgtgg aaaagtgag gaaattaaa 
      gtaaggaaa agttcaagc gatgaggaa aatgcgaag 
      aagatgtgg aagattttt gttaaattt ccaaggact 
      gtcgaggaa ccggaagtt tgcatcgtt ttggaaatt 
      tttacgttg gtgagagtc gaattggca cggaagcac 
      aaaacgaac aaattggac gcgaaattg ttgaggccg 
      atacaactc gtgtaa
    mRNA sequence
    • >TC034042 RA
      ccactttac tggcacttg aaaaatgtg acgtttatc 
      gacatttca caatatttt ttcactctt acttcgcca 
      caaacttat caaaatgac aaattcccc gggaccaag 
      cacctcaac gaccgtcaa gacaaccga ccctgacct 
      taagaagaa aaaacgcac gcgtcggaa gccccgcac 
      ccgctccaa gaaagacac tgactcgcc ccccgacgt 
      cgacctgct gcctctact gccaacttt ccacattca 
      accggtcgg cagccccca tgccactgt ctacaaact 
      gcgagacca gtcccaagg ccccatcaa cttacagtc 
      tttgctcaa gtgcagttt atttcacac gagagtgcc 
      aagctgtga ccccaactc ggccctaaa cctcttaca 
      gaaaatttc caatttggc tgttaatgg aattcgcga 
      agctagttc ctcgaaaaa gaagaaagg gaagcggaa 
      agaacgtgt ttttttccc ggagtattt aagcgaggc 
      ggaggtcga gtccgggct ggaaacaag ggagttgat 
      gaagggtta tattaggat taaccccaa aaatgcgaa 
      agaaagtta tgtgggaaa tgaggatgt ttccaagac 
      ggattatat gattacgtc cgttttgga ccggaaccg 
      ggcctttga cggcgacga ggtcgtttt gaggctcaa 
      aacagaaac ggaagataa gaaaaagac catggaggt 
      tgtttacat cacaaagaa gattcaccc ccggaccgc 
      tgttggttt ccttgcccc cattgaaaa gaataaaga 
      gtacgccct gttcacccc acgcgacaa acgcatccc 
      gaaaatcaa aattccgag aataagttg gccaagtgg 
      tttccaatc caagcccca actatacca agacattct 
      tttcatcgt taaaataac ggattggaa agacgtaaa 
      gtatgccat aggcacgat taccgagaa tttgggttt 
      gagtggtga tctcaaaat cgagtcttt ggcgatttt 
      gcgcgaatt ttgtctcga tccgactcc gttcggtga 
      cgatatcag gccattcct gccaaaaag tacgaaaat 
      ttcaacaag tgacttaaa aaatcgtga ggatttacg 
      caaagagtg tgtttttac gattgatcc aatcacagc 
      acgcgattt ggacgatgc tttgtcgtg caaggagtt 
      gccaaatgg aaattacga agttggcgt tcacatttc 
      cgatgcttc gtttttcct tccggaaaa cacgcgact 
      ggacgaaat tgtgaaaag caaagctac tacgatcta 
      catggttga cagtgtgta tcacatgct tccggtgga 
      gttgtgctt ccattgttc gctacttcc gggtgaaga 
      taaactcgc atttagtgt tttttggga gttgacacc 
      gactggtga tatagtcga ttttcggat caagcgatc 
      agtgatcaa ttcatgtgt tcaactagc ctacgagca 
      tgcgcaagt catgattga tgatccgga ccggaagtt 
      tgaacgaga cgagcttcc ggagattta caacgggtt 
      taatgcaca tgatctcag taagcgggt caatgattt 
      gcaaaggat tgccgtttg tttgaggga gaaacggag 
      gcaaaatgg gtcgttgaa aattaatca aattaaaat 
      tggtttcgt gctggatcc agcaaccgg agaaccgaa 
      agagtttta catttgtga aaataaacc agcgcatag 
      attaataga ggagtttat gcttttggc caacatgac 
      cgtggccaa gtggataaa tgaggccta cccggacct 
      ggcctttct gagacttca cgaggcgcc caagccgac 
      catgatgac cgagctcca aaaaagcct cgaaaccct 
      gggcctcca catcgacat ctccacttc ggggggcat 
      ccaagcctc cttgaacaa gtacatcag tgaggacga 
      ttttggcaa agcccgcat gatcgtcct caaccacct 
      cctggccaa gcctatgaa aagggcgcg atatttttg 
      tgccggtgc ccttgagga ggagctcga ttacagtca 
      ttacgccct cagtatacc catctacac ccactttac 
      gtcaccaat ccgtcgata tgctgacat aatggtgca 
      cagactgct cgcagcgag tttaaacta tcgagagaa 
      acccacttg ggcgcctga ggaggtggc aacgattgc 
      cgaaacgtg taaccggca gaaatatca cgccaagcg 
      ggccgggga ggctagtag tgacctgtt tttggccca 
      ttttgtcga aaagaatca accagtgat tgaaaatgc 
      tgtggttgt ggatgcgaa agaaaggtc tattgatgt 
      tattgtgat taggacggg aagtgttgt caggattta 
      tacaaataa ttttgggga taaagttac gtggaaaag 
      tgaggaaat taaagtaag gaaaagttc aagcgatga 
      ggaaaatgc gaagaagat gtggaagat ttttgttaa 
      atttccaag gactgtcga ggaaccgga agtttgcat 
      cgttttgga aatttttac gttggtgag agtcgaatt 
      ggcacggaa gcacaaaac gaacaaatt ggacgcgaa 
      attgttgag gccgataca actcgtgta aggcgagtt 
      caaagcaat taaaaaaaa ttaaaataa acgaactgt 
      taaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY