Status
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Gene information
General information
TC006314 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7125151..7139404
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006314
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04108

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006314 RA
      MNDREKSRS SGKQHIYEG TFDYTLPRP PALGEERAY 
      NASERGAQP LQMDRRHYK SSSPVPKFD FDDGSSYRP 
      TSRPSSRHG KPPAYLAWA ESLHNLLQD SDGVKLFLR 
      YLESEGPHH ADTLRFWFA CEGLKNHDQ VENIQKLVK 
      LIYKTYFLK SALQINDDL KKQIMRNLK SPQCLEPPI 
      TLFDEAQAQ IENLINKTT YPNFLKSDI YLQYVQSVE 
      NASSDDFNS ESSSSSSSL SNKDLGNLA SGLGPLPTL 
      HEGMEFSLN TQQPVILTP GAASVSTGY HTPTVRLTK 
      DMLLRSQKH RALDIRPKS ETYAGLIYR SSGAHVAYN 
      SYNPVSRQD SEMQSLSSH SDARTESDN MSLNESNVD 
      GRPGRSLRK SIEEKQMRQ FARNKEVNM NQRFIPRTQ 
      RMDVGNNCL PIEEHAALL IKKLENLKR EQENQELFK 
      RRLKEGDSL SSLDDKFQT RDIESAMRE KLQFQDDND 
      QDILDEHVS RVFSDHSLS ISPGLVSPK ASSPPRGRW 
      SQSQARRRD KDVFSIFSS DSGNVHDFA ESSERLPKS 
      KSVPEYNDD RFSRAASCR RSAKKTLTD LTDSGVSVV 
      SDTASVVPV PKDSRVLTW LMESDRSAK TAPSHAHSE 
      MAVGRKYAK RYGSRSNSL ERPGPAQPF IADPSMPPL 
      PSPNTDIQL EEIRRRLTE DDGGRRSKQ RSSKYYPEV 
      AQSGQSTLR KSTRGQKPT PEDMTIVVF SFCDEQFPY 
      RTKIPGSQI TLRQFKEYL PKKGNYRFF FKTVCEDQV 
      THEEVSNDS EVLPLWEGK IMAQVKPID
    CDS sequence
    • >TC006314 RA
      atgaacgac cgggaaaag agcaggtcc agtggcaaa 
      cagcacatc tatgaaggc actttcgac tacactttg 
      ccgaggccc cccgcattg ggagaggag agagcttac 
      aacgcgtcg gagcggggg gcgcagcca ctgcagatg 
      gaccgcagg cactacaag tcttcctct cctgttcca 
      aagtttgat ttcgacgat ggtagcagt tacaggcct 
      acttcgcgg ccttcgtcg cgccacggg aaaccgccg 
      gcctacctc gcatgggcc gaatcgctg cacaatctg 
      ctgcaggat tcggacggt gtgaaatta ttcttgcgt 
      tacctcgaa tcggaaggg ccccatcat gccgacacg 
      ttgcgcttt tggttcgct tgcgaaggc ttgaagaac 
      cacgatcaa gtcgaaaat atacaaaag ttggttaaa 
      ttgatctac aaaacgtat tttttgaaa tcggcccta 
      caaatcaac gacgatctg aagaaacaa ataatgaga 
      aatttaaaa tcgccccag tgccttgaa ccacccatt 
      actctgttc gacgaggcc caagcacaa atcgaaaac 
      cttatcaat aaaactact tatcccaac ttcctcaaa 
      agcgacatc tatctgcaa tatgtccaa tcggtcgag 
      aatgcgagc agcgacgac ttcaacagc gaatcgagc 
      tcttccagc agttccctc agtaacaaa gacttgggg 
      aacttggcc agcggtttg ggccccctt cccacgctc 
      cacgaaggc atggaattc agtctcaac acccagcag 
      cccgtcatc ctaacgcca ggtgcagcc agtgtcagt 
      acgggttat cacacgcca acagttcgc ctcaccaaa 
      gacatgctc ctccgatct caaaagcat cgcgctctc 
      gacatccgc cccaaatcc gagacctat gctggtttg 
      atataccga agcagtggg gctcacgtc gcatacaat 
      tcctacaac ccggtctcg aggcaagat agcgaaatg 
      cagtcgctt agtagtcat tcagatgcg aggaccgaa 
      tcggacaat atgtcctta aatgagagc aatgttgac 
      ggcagacca ggtcgttct ttacgcaaa tccatcgaa 
      gagaaacaa atgcgtcaa ttcgcccgt aataaagaa 
      gtaaacatg aaccaacgt ttcatccca cgaacccaa 
      cgcatggat gtgggtaac aattgcctg ccaatcgaa 
      gaacacgcc gctctcttg ataaaaaag ctcgaaaat 
      ctcaaacgc gaacaggag aaccaggag ttgttcaag 
      cgccgattg aaagaaggc gattcgctg agttcgctg 
      gatgacaaa ttccaaacg cgcgatatc gagtcggcc 
      atgcgcgag aaactacag tttcaggat gacaacgat 
      caggacatc ctcgacgag cacgtctct cgtgtgttt 
      tcggaccac tcgttgtcg atctcgccg ggattggtc 
      agtccgaag gcgtcgtcc ccgccccgc ggccgctgg 
      agccaatcg caggcgcgt cgtcgcgac aaggacgtg 
      ttttcgatc tttagcagc gattcgggc aacgttcac 
      gatttcgcg gagtcgtcc gaacgcctc ccgaagtcg 
      aaaagcgtt cccgagtac aacgacgat cggttttcg 
      cgggcggcg tcctgtcga cgatcggcg aagaagacg 
      ctcacggac ttgaccgac agcggcgtt tcggtcgtt 
      tccgacacg gcgtcggtc gtgcccgtg ccgaaagac 
      agtcgtgtg ctgacgtgg ctgatggag tcggaccgc 
      agcgctaag accgcccct agccacgcc cacagcgag 
      atggcagtg gggaggaag tacgccaag aggtacggt 
      tcaaggtcg aattcgctg gaacggcca ggaccggcg 
      cagcccttc atcgccgat ccgagtatg ccgccgttg 
      ccgtccccg aacactgac atacaattg gaggagatc 
      cggaggcgg ctgacggag gacgacggc gggaggcgg 
      tccaaacag aggtcgtcg aaatactac ccggaggtg 
      gcgcaaagc ggccaatca acgttgcgc aagtcgacc 
      cgagggcaa aaaccaacc cctgaggac atgacgatc 
      gtcgttttt tcgttctgc gatgagcag tttccatac 
      agaacgaaa attccagga tcgcaaatt accctacgc 
      cagttcaaa gaatacttg ccgaaaaaa ggaaattat 
      cggtttttc ttcaagacg gtttgcgaa gatcaagtg 
      acccacgag gaggtgagc aacgacagt gaagttctt 
      ccattgtgg gaagggaaa ataatggca caggtgaag 
      ccgatcgac tga
    mRNA sequence
    • >TC006314 RA
      caacagttg gcacgttgg tggcgccat ccctcattc 
      ttcatggca tggcggcct caacatata cgtgataaa 
      aagtgcttc tttaaagtt tctttagtt attattaaa 
      aactacagt taatttccc cctttttcc tcctcgaag 
      aagtgtttc cgttcgtcc gttccacca ccacgtcgc 
      ttccgtaaa aatgttcca gtgttgaaa aaatgtgtt 
      gtgtttatg ttttccgta caaaaataa cattcggat 
      tttcaccgg acgcgaaaa agttttcat gatgaatcc 
      gaggcgttc cgccccctg taagtgccc catctcgct 
      cggctcgta tcgggggcc tctctcaac atgaacgac 
      cgggaaaag agcaggtcc agtggcaaa cagcacatc 
      tatgaaggc actttcgac tacactttg ccgaggccc 
      cccgcattg ggagaggag agagcttac aacgcgtcg 
      gagcggggg gcgcagcca ctgcagatg gaccgcagg 
      cactacaag tcttcctct cctgttcca aagtttgat 
      ttcgacgat ggtagcagt tacaggcct acttcgcgg 
      ccttcgtcg cgccacggg aaaccgccg gcctacctc 
      gcatgggcc gaatcgctg cacaatctg ctgcaggat 
      tcggacggt gtgaaatta ttcttgcgt tacctcgaa 
      tcggaaggg ccccatcat gccgacacg ttgcgcttt 
      tggttcgct tgcgaaggc ttgaagaac cacgatcaa 
      gtcgaaaat atacaaaag ttggttaaa ttgatctac 
      aaaacgtat tttttgaaa tcggcccta caaatcaac 
      gacgatctg aagaaacaa ataatgaga aatttaaaa 
      tcgccccag tgccttgaa ccacccatt actctgttc 
      gacgaggcc caagcacaa atcgaaaac cttatcaat 
      aaaactact tatcccaac ttcctcaaa agcgacatc 
      tatctgcaa tatgtccaa tcggtcgag aatgcgagc 
      agcgacgac ttcaacagc gaatcgagc tcttccagc 
      agttccctc agtaacaaa gacttgggg aacttggcc 
      agcggtttg ggccccctt cccacgctc cacgaaggc 
      atggaattc agtctcaac acccagcag cccgtcatc 
      ctaacgcca ggtgcagcc agtgtcagt acgggttat 
      cacacgcca acagttcgc ctcaccaaa gacatgctc 
      ctccgatct caaaagcat cgcgctctc gacatccgc 
      cccaaatcc gagacctat gctggtttg atataccga 
      agcagtggg gctcacgtc gcatacaat tcctacaac 
      ccggtctcg aggcaagat agcgaaatg cagtcgctt 
      agtagtcat tcagatgcg aggaccgaa tcggacaat 
      atgtcctta aatgagagc aatgttgac ggcagacca 
      ggtcgttct ttacgcaaa tccatcgaa gagaaacaa 
      atgcgtcaa ttcgcccgt aataaagaa gtaaacatg 
      aaccaacgt ttcatccca cgaacccaa cgcatggat 
      gtgggtaac aattgcctg ccaatcgaa gaacacgcc 
      gctctcttg ataaaaaag ctcgaaaat ctcaaacgc 
      gaacaggag aaccaggag ttgttcaag cgccgattg 
      aaagaaggc gattcgctg agttcgctg gatgacaaa 
      ttccaaacg cgcgatatc gagtcggcc atgcgcgag 
      aaactacag tttcaggat gacaacgat caggacatc 
      ctcgacgag cacgtctct cgtgtgttt tcggaccac 
      tcgttgtcg atctcgccg ggattggtc agtccgaag 
      gcgtcgtcc ccgccccgc ggccgctgg agccaatcg 
      caggcgcgt cgtcgcgac aaggacgtg ttttcgatc 
      tttagcagc gattcgggc aacgttcac gatttcgcg 
      gagtcgtcc gaacgcctc ccgaagtcg aaaagcgtt 
      cccgagtac aacgacgat cggttttcg cgggcggcg 
      tcctgtcga cgatcggcg aagaagacg ctcacggac 
      ttgaccgac agcggcgtt tcggtcgtt tccgacacg 
      gcgtcggtc gtgcccgtg ccgaaagac agtcgtgtg 
      ctgacgtgg ctgatggag tcggaccgc agcgctaag 
      accgcccct agccacgcc cacagcgag atggcagtg 
      gggaggaag tacgccaag aggtacggt tcaaggtcg 
      aattcgctg gaacggcca ggaccggcg cagcccttc 
      atcgccgat ccgagtatg ccgccgttg ccgtccccg 
      aacactgac atacaattg gaggagatc cggaggcgg 
      ctgacggag gacgacggc gggaggcgg tccaaacag 
      aggtcgtcg aaatactac ccggaggtg gcgcaaagc 
      ggccaatca acgttgcgc aagtcgacc cgagggcaa 
      aaaccaacc cctgaggac atgacgatc gtcgttttt 
      tcgttctgc gatgagcag tttccatac agaacgaaa 
      attccagga tcgcaaatt accctacgc cagttcaaa 
      gaatacttg ccgaaaaaa ggaaattat cggtttttc 
      ttcaagacg gtttgcgaa gatcaagtg acccacgag 
      gaggtgagc aacgacagt gaagttctt ccattgtgg 
      gaagggaaa ataatggca caggtgaag ccgatcgac 
      tgagttttt tacgttgag aaaacgtaa aattcgtat 
      ttatttttt gttacaacg acgaaaatg gcgataatt 
      ttgcaaaat gatttttta aattagttt ttggtaaat 
      tttatagtc tgtttttgt cgttttgtt acactgcca 
      cggtatgtt gtaattgtt tttcctttt tggggggga 
      aataaagca aataaaatg cacataaat ttgtatagt 
      gctttaaaa aacgatagt aataagatg cctaattcc 
      gggactata atttttgag aaattaccg acagagtcg 
      ctttgtata taactaacg gcgattttg tctgttttc 
      ttttgcaat aggtgcaag acaagtgcc ttttttgag 
      acggtttct tcggttttt ccatcaata gagacaatc 
      agaattaag cgggcagct gattgctaa ttttatttc 
      taattttaa gtttttcaa ttttaggaa tagttcttc 
      atatacctc atttctcta tttctcttt ttagaatta 
      attctatga aaaaaacta tttgtggat ttgtttact 
      taaattata cgttttaag tgtagaact gattttgta 
      taattaatt aatgtattt tttttttga aaaatgtct 
      taagcgaaa caatacaat tgatgcaat ttcggcgag 
      ttttattta atttttatt cctctattt ttagagtat 
      ttttcagag tattacgta ttttaggtg ccataaccg 
      aattcttat cgtttgcag tggcgtaga ttacgaaat 
      ttttacaaa taactcgag tttttatta taaaatgcg 
      actttgggg ctccccatg gtagtaaca ttgttacga 
      aataaaata gttttttta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY