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Gene information
General information
TC006313 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7118421..7122485
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006313
    iBeetle screen iB_04107

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006313 RA
      MVKNQANDD SNEKKSHRD RHSGRKAEK KKGKKNANR 
      LEDVPDKQR NPKAFAFNS AVRAERKFR RKQDIDTKK 
      QHVPLVDRT PLEPPPILI AVVGPPKVG KSTLINNLI 
      KLFTKSPLV EIKGPVTIV TGKKRRITF IECNNDINS 
      MIDLAKVAD LVLLLCDAS FGFEMEVFE FLNICQVHG 
      MPKIMGILT HLDMIKNAK TLKNTKKVL KHRFWTEVY 
      PGAKLFYLS GLVHDEYLR NEIRNLGRF ISVMKFRPL 
      TWRTTHGYL LADRYEDLT NQELVRQNP KCDRNISLY 
      GYVRGVAIK NHASIHIAG LGDVKIHDV SFLPDPCPL 
      PEQIKKRAL VEKEKLIYA PFSGVGGIV YDKDAVYVE 
      LGGSHSHSK KNEDETDIV TNLIDTQKT LDVKMAHSK 
      IQIFTGGKE ITGEEFGAG SDEEEEEEQ ETETKLYEK 
      KEEEKEEED LEGELKKLR GAYERFQEE RVEDDGRVR 
      RRVVFETGD EQLANRIED SDGEDSDDS EQKIKNEEN 
      KDKKRMKKT DCDNERKMT NFKEENTDD EDSDEENTD 
      DEDLNDFAT EENEDEVLV RKKNETENK DDVHSKISN 
      ILKKLDEKK EATLVEESS DEFEEQEDE NHDEDASES 
      EDLDEDSED NDDVKWKDN LAEKAHSAF LDRQSTNQN 
      LMKLVYGVF EKNNRNEDE ETQTEPEED EEETIGGLF 
      KKVSRAQQK LKLDKDTMN LPESSLIQP WNAPVRDYL 
      TEENKTTIV NCFVTGKWK SSEDASELL KLDDAEDLS 
      DMDDSEMFG DFEDLETGE KHTKRKRDE VEKDEEADR 
      QALAEKKRK LKEKFDEEY DNTEKSSYY DDLKQSAEK 
      QAQLNKTVF ENMADDVRV QIEGFRPGM YVRIEIRDV 
      PAEFVTNFN PTYPLIVGA LNMGEENIG YVNVKIKKH 
      RWYSKILKT GDPLIVSLG WRRFQTLPI YSKLEDDLK 
      FRYLKYTPE HLACNAHFW GPITPQGSG FVALQSVAS 
      NPEILKKQG FRIAATGVV QELDKSTQI MKKLKLVGH 
      PLKIYKKTA FIKGMFNTA LEVAKFEGA RIKTVSGIR 
      GQIKKAVNK PEGCFRATF EDKILLSDI VFCRTWYKV 
      DVPQFYTPV TTLLLPEDR KNSWRGVRT TGEIKREKG 
      VKNLPNEDS LYRPIERQP KPFKPLVIP AKLQKALPY 
      RDKPKHGVK ATDKKRGID RVAVIREPH EQKVSNLMK 
      MVKTNYEYK QEKVKAETQ ERLQKHRQM VEAQETMKE 
      KKIKQKKKE VFRKKSKAQ NSKK
    CDS sequence
    • >TC006313 RA
      atggtaaaa aaccaagcg aacgatgac tcaaatgag 
      aaaaaatcg caccgggac cggcattca ggtcgcaag 
      gctgagaag aagaagggc aaaaagaat gcgaaccgt 
      ttggaagac gtgcctgat aagcagcgc aatcccaaa 
      gcctttgcc ttcaattcg gccgtgcgt gccgagcgt 
      aaattccgc cggaaacag gacatagac acgaaaaaa 
      cagcatgtt cctcttgtc gatcgcacc cccttggag 
      ccccctccg attttaatc gcggtggtg ggccccccg 
      aaagtgggc aagagcacc ctaatcaac aacttgatc 
      aagttgttt acaaaatcg ccccttgtc gaaatcaag 
      ggccccgtt acgatagtc accggcaaa aaacgccga 
      atcactttc atcgagtgc aacaacgat atcaacagc 
      atgatcgac ctggcgaaa gtggccgat ttggtgctg 
      ttactgtgt gacgcgagt ttcggcttc gaaatggaa 
      gtgttcgag tttttaaac atttgccaa gtccatgga 
      atgcccaaa atcatgggc atcttgacc catctagac 
      atgatcaaa aacgcgaaa actctcaaa aacacgaaa 
      aaagtccta aaacaccgg ttctggacc gaagtgtac 
      cccggcgct aaacttttc tacctctcc ggactggta 
      cacgacgaa tatttgcgt aatgaaatt cgcaatttg 
      gggcgtttc atctccgtg atgaagttc cgccctctg 
      acgtggcgc accactcac ggctatttg ttagccgat 
      cgctacgag gatttaacc aatcaggaa ctggtccgg 
      caaaacccg aagtgcgat cgtaacatc tcgctttat 
      gggtatgtc agaggcgtg gcgattaaa aaccacgcc 
      tccattcac attgccggg ttgggtgat gttaaaatc 
      cacgacgtt tcgtttttg cccgaccct tgcccactc 
      cctgaacaa atcaagaag cgggcattg gtcgaaaag 
      gagaagctg atttacgcc cctttttcg ggagtgggg 
      gggattgtc tatgacaaa gacgcggtt tatgtcgag 
      ttggggggc tcacacagt catagtaag aaaaacgag 
      gatgagact gacattgtt accaacttg atcgatacg 
      cagaagacg ctagatgtg aaaatggcg cattcgaaa 
      atacagatt ttcacggga gggaaggag ataacgggg 
      gaggaattt ggggctggt agtgacgag gaggaggag 
      gaggagcaa gaaacggaa acgaaactt tacgagaag 
      aaagaagaa gaaaaggag gaggaagat ttggagggt 
      gaattgaag aaattgagg ggggcgtat gaacggttt 
      caggaagaa agggtggag gatgatggg agggtcagg 
      aggagggtc gtttttgag acaggagat gagcaatta 
      gccaatagg attgaagac tcagatggt gaggactcg 
      gatgattca gaacaaaaa attaagaat gaagaaaat 
      aaggataaa aagagaatg aagaaaaca gattgtgac 
      aatgaacgc aaaatgacg aattttaaa gaagaaaat 
      actgatgat gaagattcg gatgaagaa aatacagat 
      gatgaggat ttgaatgat tttgcgaca gaagaaaat 
      gaagatgaa gtgcttgtg agaaagaag aatgaaaca 
      gaaaacaaa gatgatgtc cattcgaaa attagcaat 
      attttgaag aaactggac gagaagaag gaagctaca 
      cttgttgaa gagtcttcg gatgaattt gaagaacaa 
      gaagacgaa aatcatgat gaagatgcg tcagaatct 
      gaagattta gatgaagac tctgaagat aacgacgac 
      gtaaaatgg aaagataat ctggcggaa aaagcacac 
      agtgccttc ttggatcgc cagagcacc aatcagaac 
      ctcatgaag ctagtttat ggcgttttc gaaaagaat 
      aacagaaac gaagacgaa gaaacacaa actgaaccg 
      gaagaagac gaagaagaa acgatcggt ggtctcttc 
      aagaaggtt tctcgcgcc cagcaaaag ctcaaactt 
      gacaaggac acaatgaac cttcctgaa tcttcctta 
      atacagccc tggaatgcc cctgtcaga gactacctg 
      accgaagaa aacaaaaca acaattgtc aattgtttc 
      gttaccggc aaatggaag agttcggaa gacgcttcg 
      gagctttta aaattagac gatgccgaa gatttaagc 
      gatatggat gattcagaa atgttcggt gattttgaa 
      gatctggaa acgggtgaa aaacacacg aagcggaaa 
      cgcgacgaa gtggagaaa gacgaagag gccgacagg 
      caagccttg gccgagaag aagcgcaaa ttgaaggag 
      aaattcgat gaagaatat gacaacacg gagaagagc 
      tcctattat gatgatttg aaacaaagc gcggaaaag 
      caagcacag ctgaataaa accgtcttt gagaacatg 
      gccgatgat gtccgagtg cagatcgag ggtttccgg 
      ccggggatg tacgtccga atcgagatt cgggacgtt 
      ccggctgaa ttcgtgacg aacttcaac ccgacttac 
      cctctgatc gtcggcgct ctcaacatg ggggaggaa 
      aatatcggg tacgtcaac gtgaaaata aagaagcat 
      cgctggtat agcaagatt ctcaaaacg ggggatccc 
      ctcattgtt tcgctcggc tggcggcgc tttcaaacc 
      ctccccatt tattccaaa ctcgaggac gatttaaag 
      tttcgttac ctgaagtac acgcctgag cacttggcg 
      tgcaatgcg catttttgg ggccccatt acgccccaa 
      ggttcgggt ttcgttgct ctgcagtca gtcgcgtcc 
      aatccggag attttgaaa aagcaaggc ttccgtatc 
      gctgccaca ggcgtggtc caggagttg gacaagtcg 
      acgcaaatc atgaaaaag ttgaagttg gtgggacac 
      cccttgaaa atttacaaa aagacggct ttcattaaa 
      ggcatgttt aatacagcc ctcgaagtg gcgaagttt 
      gagggggcg aggatcaag acagtgtcg gggatcagg 
      gggcagatt aagaaggcg gtgaacaag cctgagggc 
      tgtttcagg gccacgttc gaggataag attttgttg 
      agtgatatt gtcttttgc cggacgtgg tataaagtc 
      gatgtgccc cagttttat actcccgtg accacgctt 
      ttgctgccg gaggacagg aagaactcg tggcgcggt 
      gttagaacg actggggaa atcaaacgg gagaaagga 
      gttaaaaat ttgcccaat gaggatagt ttatatcgg 
      ccaattgag agacaacct aagccgttc aaacccttg 
      gtcattcct gccaagcta caaaaagcg ctaccgtac 
      cgtgataag cccaaacat ggcgtcaag gccactgat 
      aagaagagg ggcattgat agggtggcc gtgatccgc 
      gagccccac gaacaaaag gtctcgaac ttgatgaaa 
      atggttaaa acaaattac gagtataaa caagagaag 
      gttaaagcc gagacccag gagcgacta cagaagcac 
      cgacaaatg gtcgaagca caagaaaca atgaaggaa 
      aagaaaatc aaacagaag aaaaaggaa gtgttccgc 
      aagaaaagc aaagcccaa aatagcaaa aaataa
    mRNA sequence
    • >TC006313 RA
      taattcgta aaatacgaa gaagagcct taattacgc 
      aaaaattga ttttgtcaa gttggtagc tctaaacca 
      atgtcaact cgcaaaacg tgagctaac ctcaaaaat 
      acatgtgtt gcgattttg gtgtttact tggcaaaaa 
      atggtaaaa aaccaagcg aacgatgac tcaaatgag 
      aaaaaatcg caccgggac cggcattca ggtcgcaag 
      gctgagaag aagaagggc aaaaagaat gcgaaccgt 
      ttggaagac gtgcctgat aagcagcgc aatcccaaa 
      gcctttgcc ttcaattcg gccgtgcgt gccgagcgt 
      aaattccgc cggaaacag gacatagac acgaaaaaa 
      cagcatgtt cctcttgtc gatcgcacc cccttggag 
      ccccctccg attttaatc gcggtggtg ggccccccg 
      aaagtgggc aagagcacc ctaatcaac aacttgatc 
      aagttgttt acaaaatcg ccccttgtc gaaatcaag 
      ggccccgtt acgatagtc accggcaaa aaacgccga 
      atcactttc atcgagtgc aacaacgat atcaacagc 
      atgatcgac ctggcgaaa gtggccgat ttggtgctg 
      ttactgtgt gacgcgagt ttcggcttc gaaatggaa 
      gtgttcgag tttttaaac atttgccaa gtccatgga 
      atgcccaaa atcatgggc atcttgacc catctagac 
      atgatcaaa aacgcgaaa actctcaaa aacacgaaa 
      aaagtccta aaacaccgg ttctggacc gaagtgtac 
      cccggcgct aaacttttc tacctctcc ggactggta 
      cacgacgaa tatttgcgt aatgaaatt cgcaatttg 
      gggcgtttc atctccgtg atgaagttc cgccctctg 
      acgtggcgc accactcac ggctatttg ttagccgat 
      cgctacgag gatttaacc aatcaggaa ctggtccgg 
      caaaacccg aagtgcgat cgtaacatc tcgctttat 
      gggtatgtc agaggcgtg gcgattaaa aaccacgcc 
      tccattcac attgccggg ttgggtgat gttaaaatc 
      cacgacgtt tcgtttttg cccgaccct tgcccactc 
      cctgaacaa atcaagaag cgggcattg gtcgaaaag 
      gagaagctg atttacgcc cctttttcg ggagtgggg 
      gggattgtc tatgacaaa gacgcggtt tatgtcgag 
      ttggggggc tcacacagt catagtaag aaaaacgag 
      gatgagact gacattgtt accaacttg atcgatacg 
      cagaagacg ctagatgtg aaaatggcg cattcgaaa 
      atacagatt ttcacggga gggaaggag ataacgggg 
      gaggaattt ggggctggt agtgacgag gaggaggag 
      gaggagcaa gaaacggaa acgaaactt tacgagaag 
      aaagaagaa gaaaaggag gaggaagat ttggagggt 
      gaattgaag aaattgagg ggggcgtat gaacggttt 
      caggaagaa agggtggag gatgatggg agggtcagg 
      aggagggtc gtttttgag acaggagat gagcaatta 
      gccaatagg attgaagac tcagatggt gaggactcg 
      gatgattca gaacaaaaa attaagaat gaagaaaat 
      aaggataaa aagagaatg aagaaaaca gattgtgac 
      aatgaacgc aaaatgacg aattttaaa gaagaaaat 
      actgatgat gaagattcg gatgaagaa aatacagat 
      gatgaggat ttgaatgat tttgcgaca gaagaaaat 
      gaagatgaa gtgcttgtg agaaagaag aatgaaaca 
      gaaaacaaa gatgatgtc cattcgaaa attagcaat 
      attttgaag aaactggac gagaagaag gaagctaca 
      cttgttgaa gagtcttcg gatgaattt gaagaacaa 
      gaagacgaa aatcatgat gaagatgcg tcagaatct 
      gaagattta gatgaagac tctgaagat aacgacgac 
      gtaaaatgg aaagataat ctggcggaa aaagcacac 
      agtgccttc ttggatcgc cagagcacc aatcagaac 
      ctcatgaag ctagtttat ggcgttttc gaaaagaat 
      aacagaaac gaagacgaa gaaacacaa actgaaccg 
      gaagaagac gaagaagaa acgatcggt ggtctcttc 
      aagaaggtt tctcgcgcc cagcaaaag ctcaaactt 
      gacaaggac acaatgaac cttcctgaa tcttcctta 
      atacagccc tggaatgcc cctgtcaga gactacctg 
      accgaagaa aacaaaaca acaattgtc aattgtttc 
      gttaccggc aaatggaag agttcggaa gacgcttcg 
      gagctttta aaattagac gatgccgaa gatttaagc 
      gatatggat gattcagaa atgttcggt gattttgaa 
      gatctggaa acgggtgaa aaacacacg aagcggaaa 
      cgcgacgaa gtggagaaa gacgaagag gccgacagg 
      caagccttg gccgagaag aagcgcaaa ttgaaggag 
      aaattcgat gaagaatat gacaacacg gagaagagc 
      tcctattat gatgatttg aaacaaagc gcggaaaag 
      caagcacag ctgaataaa accgtcttt gagaacatg 
      gccgatgat gtccgagtg cagatcgag ggtttccgg 
      ccggggatg tacgtccga atcgagatt cgggacgtt 
      ccggctgaa ttcgtgacg aacttcaac ccgacttac 
      cctctgatc gtcggcgct ctcaacatg ggggaggaa 
      aatatcggg tacgtcaac gtgaaaata aagaagcat 
      cgctggtat agcaagatt ctcaaaacg ggggatccc 
      ctcattgtt tcgctcggc tggcggcgc tttcaaacc 
      ctccccatt tattccaaa ctcgaggac gatttaaag 
      tttcgttac ctgaagtac acgcctgag cacttggcg 
      tgcaatgcg catttttgg ggccccatt acgccccaa 
      ggttcgggt ttcgttgct ctgcagtca gtcgcgtcc 
      aatccggag attttgaaa aagcaaggc ttccgtatc 
      gctgccaca ggcgtggtc caggagttg gacaagtcg 
      acgcaaatc atgaaaaag ttgaagttg gtgggacac 
      cccttgaaa atttacaaa aagacggct ttcattaaa 
      ggcatgttt aatacagcc ctcgaagtg gcgaagttt 
      gagggggcg aggatcaag acagtgtcg gggatcagg 
      gggcagatt aagaaggcg gtgaacaag cctgagggc 
      tgtttcagg gccacgttc gaggataag attttgttg 
      agtgatatt gtcttttgc cggacgtgg tataaagtc 
      gatgtgccc cagttttat actcccgtg accacgctt 
      ttgctgccg gaggacagg aagaactcg tggcgcggt 
      gttagaacg actggggaa atcaaacgg gagaaagga 
      gttaaaaat ttgcccaat gaggatagt ttatatcgg 
      ccaattgag agacaacct aagccgttc aaacccttg 
      gtcattcct gccaagcta caaaaagcg ctaccgtac 
      cgtgataag cccaaacat ggcgtcaag gccactgat 
      aagaagagg ggcattgat agggtggcc gtgatccgc 
      gagccccac gaacaaaag gtctcgaac ttgatgaaa 
      atggttaaa acaaattac gagtataaa caagagaag 
      gttaaagcc gagacccag gagcgacta cagaagcac 
      cgacaaatg gtcgaagca caagaaaca atgaaggaa 
      aagaaaatc aaacagaag aaaaaggaa gtgttccgc 
      aagaaaagc aaagcccaa aatagcaaa aaataatat 
      attttgtac aatttattc acatttgat tttttaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY