| General information | |||
| TC034040
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 7082076..7105460 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034040 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04106 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg not developed/not fertilized -
Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
remarks: entire repetition development too advanced,
Pupal morphology
remarks: entire repetition development too advanced,
Adult morphology
remarks: entire repetition development too advanced,
- >TC034040 RA
MSLHDEIPA FSIWETLSA TIPALMSMK PSEKSAEHL AKIIKSGDF TTIFVYSRD PLSSLDDYR RIAGKYKSF TTWFLGRFF YLLSCEKLC KSHNVYVEI QLCVLEQLS NTQLSVYDE LARDYCETL NSLCHFVKS GHSTLTLNV FVPEKYADL CQKLNLEPI AIEINQRNF PDFFLNLLR IVKFILGQS VESDCYTSA TYRNLDDLL FFLSYSSVA VKIEILKIY LDLAKKFDK KNDFGDLIR FKMELFGRY FEQLVYEIY NCKIATSTK EFQSFELIT FEFFGQNLD TQIFEKLSE FLLTKTGEI APSEPLLNF CKRKRCTPL FGEYNYESF VKTALADFN TSVLCGFKS FLLTQVRNI RQEACKIKC VHSDKWKIF RGQFMQQIE ATDCTKDCK IPQFLKFLT HLATILTEV AIEMKNSNL EFFEEKIFL IKILALLNR HFATCDNNV PMPSIFDCF LSLVFVTSP PNVTFFFDM FSCIFVQNQ LSFGHFQGT SDFPRYKKE MTEFCKYVT RYAPKDVAF EYLNEFLAR ISSGFFEIA QIYEIESNF MKITKQMNN VGVVRHLAH ILTIFQNPD RVISEILTP ALSSSNERV LEEVTEALP DIICVSSSD FVKLTRIEE CQVKIDIFC KKCHDLTSD GLSVTFAKI DVDLTTLQG AILKNVLKF LTLDNAGLK SRALEVLPF CSNHILKFH SGNVAKIWV EKIGDSDEN VRKTFAKVV PKIIKCAQE NQVLSDTTK SETIEILFN CLLRLCKRS LQTSDFTLQ ETLLCTVEE ICNVRCERT VLPTIRILI YFIMVPTSK HSLIAVNKF FKLAELHDT TPSQLYKQN KREMCELIA HLCIVNQTL VNYPLATSL CKISTTLES YGSKDFVTL ECQHLLPFF VHKAIKSPL VGNLIEQMA LLIDVGLTE LLASIYGNI FLHVFLNGT KDDFKQSTL YLEKTTGLP SATLRKRNL WEILKQLLL NFHEKRDKV VLALRFLAN EDAETRTDD VREYLQPRL LGLLQHFDF KLMAKNTNR KDAILLSLA DFLTFLGAS YVTPLRFKV IAMLRTADC GDFPDLNCD VWDAFIRSC EVGTLASQL ATIFVSLLP LINSCPNKI NNIFKFLVV ENETQARDS IKDLFFVDD PKVDALVVQ TIRKYVRKF DDCGLKEKI RIFLKYLTH ETVEVRVRS LKQLKRLME KNREELDQM ILGYNGIDP IIVELIEIL TLGCREKDP VLKLACGEV IGELGAVEP AHLPRKYAQ NGRFFTFFM DDDGFITNA LSELTRALQ GEKNTVNMD RFAVAIQEI LKNHDISSD ESSARNDLW KQFPESQQE LMLPLLTSR YIVSEMQEM DFPSPIYGS PEGSTFQTW LYNWTRSLI MTLPQTNQT VLIPCLPAM KQDNRILML FLPHILLHC IVGDSERNL EKAYAEIKA VVGAKRSDF FQLRPIPSA NCGGPTLEK DIKTQCTKV VFVVLDFLD RWTREWQWS KGSTARSDP NFVKIKSFV GKFCNLELA VCNFYCGEY PRALKYLED YVAENPGEI SRQLTFLAE IYAQLDEPD GVAGVTALQ PTEPPIEQR ILSLEVSGK LSDAAACYE HVPQMKLHH LKSLIQCYL DLDNVHTAL HVAQGALNR QPEFGNMLL ELQAEPLWR LGQYDDLAT LLNRPQMTQ NNSWGVTIG RALLAFQNQ DRQAFDTII EDMKSQQVD FLGAASVEE GAYQHGYCY ISRLHALNE LQQVEKTTY EILAKADNC DFVRRVIEN LAKEWELRI KVVQESVRI IEPLLCLRR VAIEQSKRL VEDRVAVAV PMLNSLLGE CWLLSAQTA RSAGVHQQG FTHTLKAEE YNPASLFVE KSKLHWCRE EHEQALTTL RRGLEILLP DNSKEAIAA LSIEKRKIC AEAKLLIAC YNDSVSNVD TDIKTANYR EASEVYKEW ETGLVKLAQ FYDRRFENL SPEERDSSK GSEMQIWMI NCFCKSLRY GTAFVYQSM PRLLSIWFD YGTRLLDVS DAKTREERK TNLIKMTKL IDYALENLP TYVFLTAFS QIISRICHP QKEVYVELK SIIIKLLLD YPQHSLWMI ISVIKSSYK VRARRCVEI FSDSKLKSA TMLKLVNDF TSLAEKLIE LCNKSVPQD VETTTVSAL LRALPRMLA KPEFSEIMI PTHKFRKLV LPNPDFSNT QHNPFPNHY VHIVGIDEE VTLLRSLQR PRKITLRGS DGKGYIQML KPKDDLRKD FRLMEFNDI VNQLLSREP ESRQRRLNI RLYSVSPLN EECGLIEWV PNLVGLRPI LQCLYKQRG GAMTNKELR DASCNIRDP LTKKRDVFI KILLPKHPP VLGDWFRKA FPDPQSWFF ARTAYIRTT AVMSMVGYI LGLGDRHGE NILLDSTCG DTVHVDFNC LFNKGESFE WPERVPFRL TQNMVAAMG PLGVEGVFR KSCACTLRV LRTNANTLM SIVTPFVYD PLVSWPHAA NHNTAEKTN EQALDHVKN IQLRLQGII VKTKDRSFE EYR
- >TC034040 RA
atgtcacta cacgacgaa atcccggct ttttccatt tgggagacc ttaagcgcg acaattccg gccctaatg agcatgaaa ccgtccgaa aagagcgcc gaacatttg gctaaaata atcaaatcg ggggatttc acaaccatt ttcgtctac agcagggac cccctgagc agtcttgac gactaccga aggatcgcc gggaagtac aagtccttc acgacgtgg tttctcggc cgctttttc taccttctg agttgcgaa aagttgtgc aaaagtcac aatgtttac gttgagatc cagttgtgt gtcttggaa cagctatca aacacacaa ttgagcgtt tacgatgag ttggcacgg gactattgc gaaaccctg aacagtttg tgccatttt gtcaaatcc gggcacagc accttaact ctcaacgtt tttgtcccc gagaaatac gcagatttg tgccagaaa ttgaacctc gagccgatc gctatcgaa atcaatcag agaaacttc cccgatttt ttcctcaat ttgttgagg attgttaaa tttattttg ggccaaagc gtagagtct gactgttac acaagcgcc acgtaccgg aatttggac gatttgttg ttttttctt tcctattct tcagtcgcc gtaaaaatc gaaattcta aagatttat ctcgatttg gccaaaaaa ttcgacaag aagaacgat ttcggtgac ttaatccgg tttaaaatg gagctgttc gggcgatat ttcgaacag ttagtttat gaaatttac aattgtaaa atagccaca agtacaaaa gaattccaa tcgtttgaa ctcatcacg tttgagttt ttcggccag aatttggat acccaaatt ttcgaaaaa ttgagcgag tttttgctc accaaaacc ggcgaaatt gcgccatcg gagccactt ttgaatttt tgtaaacgc aagaggtgt acccccttg tttggagaa tacaattat gaaagtttc gtcaaaacg gccttagct gatttcaat acgtcggta ctttgtggt tttaagtca tttttgttg acccaagtt agaaatata agacaggag gcttgtaaa atcaagtgt gtacattcg gacaaatgg aaaattttt cgtggccaa tttatgcaa caaattgaa gccaccgat tgcactaaa gactgtaaa ataccccaa tttttgaaa tttctgacc caccttgca acaatcctt accgaagtc gcaatcgaa atgaaaaac tcgaatttg gaatttttt gaggagaaa attttcctc atcaaaatc ctggcactc ctcaatcgc catttcgcc acgtgtgac aacaacgtg ccaatgccc tcaattttc gactgtttt ttgagtcta gtcttcgtt accagtccc ccaaacgtt acttttttc ttcgatatg ttttcttgt atttttgtg caaaatcaa ctcagcttt ggacacttt caaggcacg agcgatttc ccacgatac aaaaaggaa atgaccgaa ttttgcaaa tacgtcact cgttacgcc ccaaaggac gtcgcgttt gaatacttg aatgaattt ttggcgcga atttcgagc ggttttttc gaaatcgcc caaatttac gaaattgaa tcaaacttt atgaaaatc accaaacaa atgaataac gtgggggtt gtcaggcat ttagcccac attttgaca atctttcaa aacccagac cgggtcatt tcggaaatc ctcacaccg gccctttcg tcatcaaac gaacgggtt ttggaagaa gtgaccgaa gccctgcct gacattatt tgcgtctcg tcgagtgat tttgtcaaa ctgacacga atcgaagag tgtcaagtc aaaattgac attttttgt aaaaaatgt catgacttg acaagtgac ggattgagt gtcactttt gccaaaatt gacgtcgat ttgacaact ctccaaggc gccattttg aaaaacgtc ctcaaattc ctcactctt gacaatgcg ggcctcaaa tcgagagcc ttggaggtg ttgccgttt tgctccaac cacatcctc aaattccat tcgggcaac gttgccaaa atttgggtc gaaaaaatc ggcgattct gacgaaaac gtgcgaaaa acgttcgcg aaagtggtc ccaaaaatt atcaagtgt gcgcaagag aatcaagtt ttgagcgac actactaaa agtgaaaca attgaaata ctcttcaat tgtttattg aggctttgc aagaggagt ctccaaact agcgatttt accttgcaa gagacactc ttgtgtaca gttgaggaa atttgcaac gttagatgc gagagaact gttttacca acaattcga attttgatt tatttcatc atggtgccg acgtccaaa cattcgcta atcgccgta aataaattc ttcaagttg gctgagctc cacgacacc acccccagc caactttac aagcaaaac aagcgcgag atgtgcgaa ctgatcgca cacttgtgc attgtgaac cagacttta gtaaattac ccactcgcc acctcgctg tgtaaaatc tcaacaacg ctagagtct tacggttct aaggatttt gtcactctg gagtgccaa cacctgttg ccgtttttc gtccacaag gccattaaa agcccactt gtggggaac ttgatcgag cagatggcg ctgctcatc gacgtcggt ttaaccgaa ctgttggcc agcatctac ggaaacatc ttcctccac gttttcctc aacggaacg aaggacgat ttcaaacaa tcgaccctt tatttggag aaaacaaca gggttgccg tcggcaacg ttacgaaag cgcaacctt tgggagatc cttaagcaa ctgttgctc aacttccac gaaaaacgc gataaggtc gtgttggcg ctgcgtttc ctggccaat gaggacgca gaaacgcga acagatgac gttcgcgag tacctacag ccgcgcctt ttgggcctt ttgcagcac tttgatttc aaactaatg gccaagaac accaatcgc aaggacgcg attttgctc agcttggcc gattttctc acgtttttg ggcgcgagt tatgtgacg ccgctccgg ttcaaggtt attgcaatg ctgcgaacg gccgattgc ggggacttc cccgacttg aactgcgac gtttgggac gcttttatc cgaagctgc gaagtcgga acgctggct tcgcagttg gccacgatt ttcgtctcg ttgttgcct ttaatcaac tcatgcccc aacaaaatc aacaacatc ttcaagttt ctcgttgtc gagaacgag acgcaagcc agggattcg atcaaggat ttgttcttt gttgacgat ccgaaagtc gatgctttg gtcgtgcag acgatacgg aagtacgtc cgaaagttc gacgattgc gggctgaag gagaaaata cggattttc ctcaagtat ttgacgcac gaaacggtc gaagtgcgg gtgaggagt ttgaagcag ttgaagcgt ttgatggag aaaaaccgg gaggagttg gaccagatg atactgggg tataatggg attgatccg attattgtc gagttgatt gaaattttg acgttgggg tgccgggag aaggacccc gtgcttaaa ttggcgtgt ggggaggtt attggggag ttgggggct gttgaaccc gcacatctt ccgcgaaag tacgcccag aatgggcgc ttcttcacg ttttttatg gacgatgat gggtttatt actaatgcg ttgagtgag ctcacgagg gcgctacaa ggagagaaa aacacagtt aacatggac cgcttcgcg gtcgcaatt caggaaatc ctgaagaat cacgacatt tcgtcagac gagtcaagt gctcggaac gacttatgg aaacaattc cccgaaagt cagcaggaa ttaatgttg cctttgtta acctcgcgc tatatcgtg tcagaaatg caagagatg gacttcccg agcccgatt tacggcagt ccggagggt tccaccttc caaacgtgg ctttacaat tggacccga agtctcatc atgacattg cctcaaacg aaccaaact gttttaatc ccgtgcctc cctgccatg aagcaggac aaccgcatt ttgatgctg ttcctgccc cacatcctc ctgcattgc atagttggc gattcggag cgaaatctc gaaaaggcc tacgccgaa attaaggca gttgttggg gcaaaacgc agtgatttt tttcagctt cgaccgatt ccatcggcg aattgtggc ggcccaacg ctcgaaaaa gacataaag acgcaatgt acgaaagtt gtgttcgtc gttttggat tttttggat cggtggacg cgcgaatgg caatggagt aaaggttca acggcgagg agtgatccg aattttgtc aaaattaag agttttgtg gggaagttt tgcaatttg gagttggcc gtttgtaat ttttattgc ggggagtac ccgagggcc ttgaagtat ttggaggat tacgtggcg gaaaatccg ggcgaaatt tcgcggcag ttgactttc ctggcagag atttacgcc caattggac gaacccgac ggcgtagcc ggcgtcaca gctctccaa cccaccgag cccccgatc gaacagcgc attctctcg ctcgaagtc tccgggaag ctttccgac gcggccgct tgctacgaa cacgtcccc cagatgaag ctccaccac ctcaaaagc ctcatccag tgctacctg gacctggac aacgtccac accgccctg cacgtcgcc caaggcgcc ctcaaccgc caacccgag ttcggaaac atgctcctc gaactccaa gccgagccc ttgtggcgc ctcggccag tacgacgac ctcgcaacg ctcctaaac cgcccccaa atgacccaa aacaactcc tggggcgtc acgatcgga cgagcttta ctcgcgttc caaaaccaa gaccgccag gcttttgac acaataatc gaagacatg aagagccaa caagtggat tttttgggg gctgccagt gtggaagaa ggggcctat cagcacggt tattgttac atcagtcgg ctgcacgcc ctcaatgag ttacaacaa gtcgaaaaa accacctat gaaatactc gccaaagca gataattgc gattttgtt cgtcgggtg attgaaaac ttggcgaaa gaatgggag ttgaggatc aaagtcgtg caagaatcg gtgcgcata atcgagccg cttttgtgt ctccgaaga gtggcaatc gaacagtcg aaacgtttg gttgaggat cgagtcgct gtggccgtc ccgatgctc aattctttg ttgggcgag tgttggttg ttgagtgca cagacggct agatcggct ggggtgcac cagcaaggg ttcacacat acgctcaaa gcagaggag tacaacccg gcaagtttg ttcgttgaa aaaagcaaa ttgcattgg tgtagagag gagcatgag caggcattg acgacgtta cgacggggc ttggagatt ttactgccc gataattcg aaggaggcg atcgctgcg ttgtcaatt gagaagagg aagatttgt gccgaagcg aaattgttg attgcgtgt tacaatgat tccgtttcg aatgtcgat actgatatt aaaacggcg aattatcgg gaggctagt gaggtgtac aaggagtgg gaaacgggg ctggtgaaa ctggctcag ttttacgac cgtcgcttc gaaaacctc tcacccgaa gagcgcgac agcagcaaa ggcagcgaa atgcaaatt tggatgatc aattgcttt tgcaaatcg ctacgttac ggcacggct ttcgtctac caatcaatg ccgcgctta ctatcaatt tggttcgat tacggcacg cgccttttg gacgtttcc gacgcaaaa acccgcgaa gaacgcaag accaatctg atcaaaatg acaaaactc atcgactac gcgctcgaa aacctccca acctacgtg ttcttgacc gctttctcg cagattatt tcgcgcatt tgccatcca cagaaagaa gtttacgtc gaattaaag tcgataata attaagctg ttgctcgat tatccccag cacagtctc tggatgatc atctccgtg atcaagtcg agttacaaa gtgcgcgcg cggcgttgt gtcgagatt ttcagcgat tccaagttg aaaagtgcg acaatgtta aaactggtc aatgatttt acgagtttg gccgaaaaa ctgatcgaa ttgtgtaac aaaagcgtg ccacaggac gttgaaacg acgactgtt agtgccttg ttacgggcg ctcccccga atgttggcc aagccagaa tttagcgaa attatgatc cctacgcac aagtttcgg aagttggtg ctcccgaat ccagatttt tccaacacg caacacaat cccttcccg aatcattac gtgcatatt gtggggatt gacgaggaa gtgacgctt ttgaggagt ttgcagcgg ccgaggaaa atcacgctg cggggatcg gacgggaaa ggttatatt cagatgttg aagccgaag gacgacctc cgcaaagac ttccgcttg atggaattc aacgatatt gtgaaccaa ttactgtca cgtgagccc gaatcgaga caaaggcgg ctcaatata agactatac tccgtttcg cccctcaat gaggaatgt ggtttaatc gaatgggtc ccgaacctg gtgggcttg aggcccatt ttgcaatgt ttgtacaag caacgtggg ggcgcaatg accaacaag gagctccgc gacgccagc tgcaatatc cgggacccc cttacgaaa aaacgcgac gttttcatc aaaattctc ctgccgaag caccctccc gtcttaggg gactggttc cggaaggcg tttccggac ccccagagc tggtttttc gcccggact gcgtacata aggactacg gcggttatg tcaatggtg gggtatatt ctagggttg ggcgaccga cacggtgaa aatatcctt ctggactcg acatgtggt gatacggtg catgtcgat ttcaattgt cttttcaac aaaggggag agttttgag tggccggag agggtgccc ttcagattg acgcaaaat atggtggct gcgatgggg ccactgggg gttgaagga gtgtttcgg aagtcgtgt gcctgcact ttgagggtt ttaaggaca aatgctaat actttaatg tcgattgtg acccccttt gtgtacgat ccgctcgtg tcgtggccc catgcagcc aatcacaat acagcggag aaaacaaat gaacaggcc ctagaccat gtcaaaaac atccaactg aggttgcaa ggaattatc gtgaaaacg aaggaccgc tcattcgaa gaatatcga taa
- >TC034040 RA
gggattgcg ccaaaagtc aaaaaatgt agtctgttt tcgtgaatt agacaatgt ttcgtgtaa ttatttcta gtttgtgat tttttatct cctttatgt taagtcatg tcactacac gacgaaatc ccggctttt tccatttgg gagacctta agcgcgaca attccggcc ctaatgagc atgaaaccg tccgaaaag agcgccgaa catttggct aaaataatc aaatcgggg gatttcaca accattttc gtctacagc agggacccc ctgagcagt cttgacgac taccgaagg atcgccggg aagtacaag tccttcacg acgtggttt ctcggccgc tttttctac cttctgagt tgcgaaaag ttgtgcaaa agtcacaat gtttacgtt gagatccag ttgtgtgtc ttggaacag ctatcaaac acacaattg agcgtttac gatgagttg gcacgggac tattgcgaa accctgaac agtttgtgc cattttgtc aaatccggg cacagcacc ttaactctc aacgttttt gtccccgag aaatacgca gatttgtgc cagaaattg aacctcgag ccgatcgct atcgaaatc aatcagaga aacttcccc gattttttc ctcaatttg ttgaggatt gttaaattt attttgggc caaagcgta gagtctgac tgttacaca agcgccacg taccggaat ttggacgat ttgttgttt tttctttcc tattcttca gtcgccgta aaaatcgaa attctaaag atttatctc gatttggcc aaaaaattc gacaagaag aacgatttc ggtgactta atccggttt aaaatggag ctgttcggg cgatatttc gaacagtta gtttatgaa atttacaat tgtaaaata gccacaagt acaaaagaa ttccaatcg tttgaactc atcacgttt gagtttttc ggccagaat ttggatacc caaattttc gaaaaattg agcgagttt ttgctcacc aaaaccggc gaaattgcg ccatcggag ccacttttg aatttttgt aaacgcaag aggtgtacc cccttgttt ggagaatac aattatgaa agtttcgtc aaaacggcc ttagctgat ttcaatacg tcggtactt tgtggtttt aagtcattt ttgttgacc caagttaga aatataaga caggaggct tgtaaaatc aagtgtgta cattcggac aaatggaaa atttttcgt ggccaattt atgcaacaa attgaagcc accgattgc actaaagac tgtaaaata ccccaattt ttgaaattt ctgacccac cttgcaaca atccttacc gaagtcgca atcgaaatg aaaaactcg aatttggaa ttttttgag gagaaaatt ttcctcatc aaaatcctg gcactcctc aatcgccat ttcgccacg tgtgacaac aacgtgcca atgccctca attttcgac tgttttttg agtctagtc ttcgttacc agtccccca aacgttact tttttcttc gatatgttt tcttgtatt tttgtgcaa aatcaactc agctttgga cactttcaa ggcacgagc gatttccca cgatacaaa aaggaaatg accgaattt tgcaaatac gtcactcgt tacgcccca aaggacgtc gcgtttgaa tacttgaat gaatttttg gcgcgaatt tcgagcggt tttttcgaa atcgcccaa atttacgaa attgaatca aactttatg aaaatcacc aaacaaatg aataacgtg ggggttgtc aggcattta gcccacatt ttgacaatc tttcaaaac ccagaccgg gtcatttcg gaaatcctc acaccggcc ctttcgtca tcaaacgaa cgggttttg gaagaagtg accgaagcc ctgcctgac attatttgc gtctcgtcg agtgatttt gtcaaactg acacgaatc gaagagtgt caagtcaaa attgacatt ttttgtaaa aaatgtcat gacttgaca agtgacgga ttgagtgtc acttttgcc aaaattgac gtcgatttg acaactctc caaggcgcc attttgaaa aacgtcctc aaattcctc actcttgac aatgcgggc ctcaaatcg agagccttg gaggtgttg ccgttttgc tccaaccac atcctcaaa ttccattcg ggcaacgtt gccaaaatt tgggtcgaa aaaatcggc gattctgac gaaaacgtg cgaaaaacg ttcgcgaaa gtggtccca aaaattatc aagtgtgcg caagagaat caagttttg agcgacact actaaaagt gaaacaatt gaaatactc ttcaattgt ttattgagg ctttgcaag aggagtctc caaactagc gattttacc ttgcaagag acactcttg tgtacagtt gaggaaatt tgcaacgtt agatgcgag agaactgtt ttaccaaca attcgaatt ttgatttat ttcatcatg gtgccgacg tccaaacat tcgctaatc gccgtaaat aaattcttc aagttggct gagctccac gacaccacc cccagccaa ctttacaag caaaacaag cgcgagatg tgcgaactg atcgcacac ttgtgcatt gtgaaccag actttagta aattaccca ctcgccacc tcgctgtgt aaaatctca acaacgcta gagtcttac ggttctaag gattttgtc actctggag tgccaacac ctgttgccg tttttcgtc cacaaggcc attaaaagc ccacttgtg gggaacttg atcgagcag atggcgctg ctcatcgac gtcggttta accgaactg ttggccagc atctacgga aacatcttc ctccacgtt ttcctcaac ggaacgaag gacgatttc aaacaatcg accctttat ttggagaaa acaacaggg ttgccgtcg gcaacgtta cgaaagcgc aacctttgg gagatcctt aagcaactg ttgctcaac ttccacgaa aaacgcgat aaggtcgtg ttggcgctg cgtttcctg gccaatgag gacgcagaa acgcgaaca gatgacgtt cgcgagtac ctacagccg cgccttttg ggccttttg cagcacttt gatttcaaa ctaatggcc aagaacacc aatcgcaag gacgcgatt ttgctcagc ttggccgat tttctcacg tttttgggc gcgagttat gtgacgccg ctccggttc aaggttatt gcaatgctg cgaacggcc gattgcggg gacttcccc gacttgaac tgcgacgtt tgggacgct tttatccga agctgcgaa gtcggaacg ctggcttcg cagttggcc acgattttc gtctcgttg ttgccttta atcaactca tgccccaac aaaatcaac aacatcttc aagtttctc gttgtcgag aacgagacg caagccagg gattcgatc aaggatttg ttctttgtt gacgatccg aaagtcgat gctttggtc gtgcagacg atacggaag tacgtccga aagttcgac gattgcggg ctgaaggag aaaatacgg attttcctc aagtatttg acgcacgaa acggtcgaa gtgcgggtg aggagtttg aagcagttg aagcgtttg atggagaaa aaccgggag gagttggac cagatgata ctggggtat aatgggatt gatccgatt attgtcgag ttgattgaa attttgacg ttggggtgc cgggagaag gaccccgtg cttaaattg gcgtgtggg gaggttatt ggggagttg ggggctgtt gaacccgca catcttccg cgaaagtac gcccagaat gggcgcttc ttcacgttt tttatggac gatgatggg tttattact aatgcgttg agtgagctc acgagggcg ctacaagga gagaaaaac acagttaac atggaccgc ttcgcggtc gcaattcag gaaatcctg aagaatcac gacatttcg tcagacgag tcaagtgct cggaacgac ttatggaaa caattcccc gaaagtcag caggaatta atgttgcct ttgttaacc tcgcgctat atcgtgtca gaaatgcaa gagatggac ttcccgagc ccgatttac ggcagtccg gagggttcc accttccaa acgtggctt tacaattgg acccgaagt ctcatcatg acattgcct caaacgaac caaactgtt ttaatcccg tgcctccct gccatgaag caggacaac cgcattttg atgctgttc ctgccccac atcctcctg cattgcata gttggcgat tcggagcga aatctcgaa aaggcctac gccgaaatt aaggcagtt gttggggca aaacgcagt gattttttt cagcttcga ccgattcca tcggcgaat tgtggcggc ccaacgctc gaaaaagac ataaagacg caatgtacg aaagttgtg ttcgtcgtt ttggatttt ttggatcgg tggacgcgc gaatggcaa tggagtaaa ggttcaacg gcgaggagt gatccgaat tttgtcaaa attaagagt tttgtgggg aagttttgc aatttggag ttggccgtt tgtaatttt tattgcggg gagtacccg agggccttg aagtatttg gaggattac gtggcggaa aatccgggc gaaatttcg cggcagttg actttcctg gcagagatt tacgcccaa ttggacgaa cccgacggc gtagccggc gtcacagct ctccaaccc accgagccc ccgatcgaa cagcgcatt ctctcgctc gaagtctcc gggaagctt tccgacgcg gccgcttgc tacgaacac gtcccccag atgaagctc caccacctc aaaagcctc atccagtgc tacctggac ctggacaac gtccacacc gccctgcac gtcgcccaa ggcgccctc aaccgccaa cccgagttc ggaaacatg ctcctcgaa ctccaagcc gagcccttg tggcgcctc ggccagtac gacgacctc gcaacgctc ctaaaccgc ccccaaatg acccaaaac aactcctgg ggcgtcacg atcggacga gctttactc gcgttccaa aaccaagac cgccaggct tttgacaca ataatcgaa gacatgaag agccaacaa gtggatttt ttgggggct gccagtgtg gaagaaggg gcctatcag cacggttat tgttacatc agtcggctg cacgccctc aatgagtta caacaagtc gaaaaaacc acctatgaa atactcgcc aaagcagat aattgcgat tttgttcgt cgggtgatt gaaaacttg gcgaaagaa tgggagttg aggatcaaa gtcgtgcaa gaatcggtg cgcataatc gagccgctt ttgtgtctc cgaagagtg gcaatcgaa cagtcgaaa cgtttggtt gaggatcga gtcgctgtg gccgtcccg atgctcaat tctttgttg ggcgagtgt tggttgttg agtgcacag acggctaga tcggctggg gtgcaccag caagggttc acacatacg ctcaaagca gaggagtac aacccggca agtttgttc gttgaaaaa agcaaattg cattggtgt agagaggag catgagcag gcattgacg acgttacga cggggcttg gagatttta ctgcccgat aattcgaag gaggcgatc gctgcgttg tcaattgag aagaggaag atttgtgcc gaagcgaaa ttgttgatt gcgtgttac aatgattcc gtttcgaat gtcgatact gatattaaa acggcgaat tatcgggag gctagtgag gtgtacaag gagtgggaa acggggctg gtgaaactg gctcagttt tacgaccgt cgcttcgaa aacctctca cccgaagag cgcgacagc agcaaaggc agcgaaatg caaatttgg atgatcaat tgcttttgc aaatcgcta cgttacggc acggctttc gtctaccaa tcaatgccg cgcttacta tcaatttgg ttcgattac ggcacgcgc cttttggac gtttccgac gcaaaaacc cgcgaagaa cgcaagacc aatctgatc aaaatgaca aaactcatc gactacgcg ctcgaaaac ctcccaacc tacgtgttc ttgaccgct ttctcgcag attatttcg cgcatttgc catccacag aaagaagtt tacgtcgaa ttaaagtcg ataataatt aagctgttg ctcgattat ccccagcac agtctctgg atgatcatc tccgtgatc aagtcgagt tacaaagtg cgcgcgcgg cgttgtgtc gagattttc agcgattcc aagttgaaa agtgcgaca atgttaaaa ctggtcaat gattttacg agtttggcc gaaaaactg atcgaattg tgtaacaaa agcgtgcca caggacgtt gaaacgacg actgttagt gccttgtta cgggcgctc ccccgaatg ttggccaag ccagaattt agcgaaatt atgatccct acgcacaag tttcggaag ttggtgctc ccgaatcca gatttttcc aacacgcaa cacaatccc ttcccgaat cattacgtg catattgtg gggattgac gaggaagtg acgcttttg aggagtttg cagcggccg aggaaaatc acgctgcgg ggatcggac gggaaaggt tatattcag atgttgaag ccgaaggac gacctccgc aaagacttc cgcttgatg gaattcaac gatattgtg aaccaatta ctgtcacgt gagcccgaa tcgagacaa aggcggctc aatataaga ctatactcc gtttcgccc ctcaatgag gaatgtggt ttaatcgaa tgggtcccg aacctggtg ggcttgagg cccattttg caatgtttg tacaagcaa cgtgggggc gcaatgacc aacaaggag ctccgcgac gccagctgc aatatccgg gaccccctt acgaaaaaa cgcgacgtt ttcatcaaa attctcctg ccgaagcac cctcccgtc ttaggggac tggttccgg aaggcgttt ccggacccc cagagctgg tttttcgcc cggactgcg tacataagg actacggcg gttatgtca atggtgggg tatattcta gggttgggc gaccgacac ggtgaaaat atccttctg gactcgaca tgtggtgat acggtgcat gtcgatttc aattgtctt ttcaacaaa ggggagagt tttgagtgg ccggagagg gtgcccttc agattgacg caaaatatg gtggctgcg atggggcca ctgggggtt gaaggagtg tttcggaag tcgtgtgcc tgcactttg agggtttta aggacaaat gctaatact ttaatgtcg attgtgacc ccctttgtg tacgatccg ctcgtgtcg tggccccat gcagccaat cacaataca gcggagaaa acaaatgaa caggcccta gaccatgtc aaaaacatc caactgagg ttgcaagga attatcgtg aaaacgaag gaccgctca ttcgaagaa tatcgataa tttgtgcca aatgtacat aggct
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