Status
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Gene information
General information
TC006299 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6740282..6754546
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006299
    iBeetle screen iB_04105

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006299 RA
      MNTSLSRGC SYSPRESFE YTALQFAAR RGSLEQVRY 
      LIESGTDLD TGPDPALHL ALRKQNTAI AHVLLHAGA 
      DFEIKDSHG DYPIHIACT LGLLDVVHA LCALGCSVE 
      VLTAKGLSP LHLAAKNGH IHVVRCLCA AGCNIDIRN 
      SDNIRADIT ALKYGHNDI AELLDRLRV TGQRDTLAR 
      QLVPTSKPA LRLNLRLLG HCGVGKTSL VKSLSAGLF 
      GSLFRRSSS LQSNKSRPS SPINTQIEM DVTSRQNSL 
      SFESSGNYQ STNGISVQN MDISNVGET TVWEFSGQE 
      NYFPIYHHF LWPSPHSLT LVLFSLEDS PSIQVQQVC 
      FWLNFLLAR QPADLPSSE YGQVMLVAT HVDLTRAVK 
      TQNGEWICP DAQKTLETV RKIIPHVPN LMSSVVIMD 
      TNVPASYPF KQLKSTLAE LKQECLQQT VGTWTGLLE 
      ATLAWLNSL QKDYEQFPV LSRSTFSEL LRSQVNILA 
      SDHHIQELL QQLHFMGEV FCVQDLVVV SVSWLGGQL 
      LGELFSSDF FLHARVTGV YTSEDFQAS FNQCDALGA 
      LDLLEALDV CVQCDIEGE IEYEFPIYN HVETLEGLW 
      DSEDPRYAS KGSQYGGLR LYTPPGTYH QFKSIFPHI 
      QVELRRAIL ANYSNNDSD MDLYQWHLG SKLCNTELE 
      SLITLEEDE VALQFIEIK IRGPNNSSQ YCFYFLEQI 
      MQTINHALL RVCPGLLIE KHVLSPLEL KRHSEGPFC 
      YPSDIITTA MLEAESTLD VALYNPNVD AQETIAQLI 
      MFSDLDLAG NIQWGCALR IQDLAPPIK LKLCGLLDP 
      PERHGRDWC LLALRLGLC QDKIAALDS QHSSHTMRL 
      LTTADCSIG ALITSLHEL DRLDAAEVV LRSAPIFKI 
      IEHVSV
    CDS sequence
    • >TC006299 RA
      atgaacacc tccttaagc cggggctgt tcttacagc 
      cctcgggag tccttcgaa tacactgcg cttcaattt 
      gcagctcga agaggctct ttggagcaa gtccgatat 
      ttgatcgag agtggcact gatcttgac acagggccg 
      gaccctgcc ctacatcta gccctccgg aagcaaaac 
      acggcgata gcacatgtg ctcctccat gctggggct 
      gattttgag atcaaagat tcacatgga gattatccc 
      atccatatt gcttgtact ttagggctt ttggacgtg 
      gtccatgcc ctgtgtgcg ttaggatgt agtgtcgaa 
      gttctgacg gcgaaaggg ctttcgccc ttgcatttg 
      gcggcgaag aatggacat attcatgtt gtcaggtgt 
      ttatgtgcc gctgggtgc aacatagac atccgtaat 
      tctgataac atcagggct gatatcacg gctttgaaa 
      tatggtcat aacgacatt gccgagttg ttggataga 
      ttaagggtg actgggcaa agggacact ttagcccgt 
      caattagta ccaacttcc aaaccagcc cttagatta 
      aacttgcgg cttttgggg cactgtggt gtcgggaaa 
      acttccctg gtcaagtcg ttaagtgcc ggtttgttt 
      ggttcgtta ttccggcga tcgagttcg ctccaaagc 
      aacaaatct cgtccttca tcccccatt aacacccaa 
      atcgagatg gatgtcacc tcacgacaa aattctcta 
      agttttgaa agcagtggc aattaccag tccacaaac 
      ggcattagt gtacaaaat atggacata tctaacgtt 
      ggcgaaaca acagtttgg gagttctca ggacaggaa 
      aactatttt cccatctat caccacttt ttatggcct 
      tcacctcat tcgcttact ttggttttg ttcagtttg 
      gaagactcg ccgtccatt caagtgcaa caagtttgc 
      ttctggctc aattttctt ctagcgagg caaccggct 
      gacctgcct tcttccgaa tacggccaa gtcatgttg 
      gtggccacc cacgtggac ttgacccgc gctgtgaag 
      acgcaaaac ggcgaatgg atttgtccc gacgctcag 
      aaaacgctc gaaactgtc cgaaaaata atcccccac 
      gtccccaat ctcatgtcc agtgttgtg ataatggac 
      acaaacgtg ccagcttcc taccctttc aagcaactc 
      aagtcgacg ctggccgaa ctgaaacaa gagtgcctc 
      cagcaaacc gtggggacc tggaccggc ctcctggaa 
      gcaacccta gcttggctc aactcgctc cagaaggat 
      tacgagcag tttcccgtt ttgtcgcgt tctacgttt 
      tcggagttg ttgcgaagt caggtcaac atcctggct 
      tcggaccac cacatccag gagttgctc cagcagttg 
      cacttcatg ggggaggtg ttctgcgtc caggatttg 
      gtcgtggtt tcggtttcc tggctaggg gggcaactt 
      ctaggcgag cttttctca agcgatttt ttcctacat 
      gcacgagtg actggcgtc tacactagt gaagatttc 
      caagctagt ttcaaccag tgtgacgct ttaggggcc 
      ttagattta ctcgaagct ttagatgtt tgcgttcag 
      tgtgacatt gaaggggag attgagtac gagtttcca 
      atttataat catgtggag actcttgaa gggttgtgg 
      gattcggag gatccgaga tatgcgagc aaaggatca 
      cagtatggt ggtttaagg ttgtacacc ccaccaggg 
      acgtaccac cagttcaaa tcaattttc ccccacatt 
      caagttgag ttgcggaga gcaattttg gccaattat 
      agcaacaat gatagtgat atggattta taccaatgg 
      catttaggc tcaaaacta tgtaacaca gaactcgaa 
      agtctcatt acgttggaa gaagacgaa gtcgcttta 
      caattcatt gagataaaa atcagggga ccaaataat 
      tcctcgcaa tactgtttc tactttttg gagcaaata 
      atgcaaacg ataaatcac gctttattg agagtttgt 
      cccggactg cttatcgag aaacacgtc ttgagccct 
      ttggaattg aagagacac tcagagggg cctttttgt 
      tacccctct gatatcatc acaactgca atgcttgaa 
      gctgagtca actctcgat gtcgccttg tacaacccc 
      aatgttgat gctcaagaa accatcgcc cagttaatt 
      atgtttagc gatttggat ttggcgggt aacatccag 
      tggggctgc gccctcaga atccaagac ctcgcccct 
      ccgattaaa ctgaagctg tgcggtttg ctggacccc 
      ccggagcgg cacggtcgc gactggtgc ttgctagcc 
      ctccgtttg ggtctctgt caggataaa atcgccgct 
      cttgattcg caacattcc agccatacg atgagactg 
      ctaacaaca gctgattgt tcaataggt gctcttata 
      accagtttg catgaacta gaccgactc gatgccgct 
      gaagtcgtc ctgcgttcc gcacccata tttaaaatt 
      atagagcat gtcagtgtt tag
    mRNA sequence
    • >TC006299 RA
      ttgttgcaa agtaaaaaa caccgcata cgggccatt 
      taataagga acaatgcgc acaaaagga cctttgttg 
      cacccaagt ggtttaatt catggaagt cagtttacg 
      gggaacatc tgggacgat ttagtgacc ttcggacac 
      attgttaaa aatttgtct ttgccgaaa gagtaattc 
      ctaagaaat ttctaaacg ttatccgat ccccgatta 
      aaactgtaa attcctccc acgttgtgt aaagacgag 
      ccatcagcg tgggcactc ctatcacgt gtaattaca 
      tctgaatat cctatttac gactccaca aatctcttt 
      tgatttgat tacctcctc ttgacccgt aacgagaaa 
      cctccaagc caaaaaatc tcgcatgca attattcca 
      agctcacta atgcacagt tatgtagcc tccaaatcg 
      tagagccag cctcgggac cgtttcgta tcagaaaca 
      aaccatcgg agtggatat taacccaag tcgtttgca 
      ttcatattg gcttaagga gatttcgta acataaatt 
      gaacgccgt tcctggaag cgcttttaa ttccgtttg 
      gcatttgaa tcttccaag aaaacaaat tacacgtta 
      tttcacatt cgtacacac tggatgcat gtgtgcagc 
      gcagctccg cggcatttc tcatttcaa tgcgttttt 
      aaatcacgc gaccgattt ttcgattcg aaagacagc 
      agcggttcc gagaaccga ttaatttcg atattgttt 
      gagaattca tacaatcgt tacttgcga aaatcaagt 
      ccggtttaa ttttagaaa cggactcct ttcggagaa 
      gaaattgtg caaattaag cgccaagaa ttttccatt 
      tgttctatc tttaacaaa tatttacgc gagtatagt 
      ctaggtcag gttcaattt ctgataagc gattgcggc 
      gccatcttg cgccttgac acgcctcca tttgcaggt 
      tccttcgcc cgggaaagc cggaaaatt cggaaaatc 
      tcaccatta aatcaaaca atcaactat ttcattctc 
      agtttttta gactagtgt tgaaatcaa agaaatttt 
      gcgaatttc ctttgtgac ttttccggc cgccggaaa 
      ttatcgatc ggtgatcag ctgttgttt tccgcggtt 
      gcagcacaa gtttccatg gaaaatgtg ataaaaaca 
      ctgggctta gaatgcgtt tgaaggcca gtcccttag 
      tttccctca gttttcctt taaaatgaa cacctcctt 
      aagccgggg ctgttctta cagccctcg ggagtcctt 
      cgaatacac tgcgcttca atttgcagc tcgaagagg 
      ctctttgga gcaagtccg atatttgat cgagagtgg 
      cactgatct tgacacagg gccggaccc tgccctaca 
      tctagccct ccggaagca aaacacggc gatagcaca 
      tgtgctcct ccatgctgg ggctgattt tgagatcaa 
      agattcaca tggagatta tcccatcca tattgcttg 
      tactttagg gcttttgga cgtggtcca tgccctgtg 
      tgcgttagg atgtagtgt cgaagttct gacggcgaa 
      agggctttc gcccttgca tttggcggc gaagaatgg 
      acatattca tgttgtcag gtgtttatg tgccgctgg 
      gtgcaacat agacatccg taattctga taacatcag 
      ggctgatat cacggcttt gaaatatgg tcataacga 
      cattgccga gttgttgga tagattaag ggtgactgg 
      gcaaaggga cactttagc ccgtcaatt agtaccaac 
      ttccaaacc agcccttag attaaactt gcggctttt 
      ggggcactg tggtgtcgg gaaaacttc cctggtcaa 
      gtcgttaag tgccggttt gtttggttc gttattccg 
      gcgatcgag ttcgctcca aagcaacaa atctcgtcc 
      ttcatcccc cattaacac ccaaatcga gatggatgt 
      cacctcacg acaaaattc tctaagttt tgaaagcag 
      tggcaatta ccagtccac aaacggcat tagtgtaca 
      aaatatgga catatctaa cgttggcga aacaacagt 
      ttgggagtt ctcaggaca ggaaaacta ttttcccat 
      ctatcacca ctttttatg gccttcacc tcattcgct 
      tactttggt tttgttcag tttggaaga ctcgccgtc 
      cattcaagt gcaacaagt ttgcttctg gctcaattt 
      tcttctagc gaggcaacc ggctgacct gccttcttc 
      cgaatacgg ccaagtcat gttggtggc cacccacgt 
      ggacttgac ccgcgctgt gaagacgca aaacggcga 
      atggatttg tcccgacgc tcagaaaac gctcgaaac 
      tgtccgaaa aataatccc ccacgtccc caatctcat 
      gtccagtgt tgtgataat ggacacaaa cgtgccagc 
      ttcctaccc tttcaagca actcaagtc gacgctggc 
      cgaactgaa acaagagtg cctccagca aaccgtggg 
      gacctggac cggcctcct ggaagcaac cctagcttg 
      gctcaactc gctccagaa ggattacga gcagtttcc 
      cgttttgtc gcgttctac gttttcgga gttgttgcg 
      aagtcaggt caacatcct ggcttcgga ccaccacat 
      ccaggagtt gctccagca gttgcactt catggggga 
      ggtgttctg cgtccagga tttggtcgt ggtttcggt 
      ttcctggct aggggggca acttctagg cgagctttt 
      ctcaagcga ttttttcct acatgcacg agtgactgg 
      cgtctacac tagtgaaga tttccaagc tagtttcaa 
      ccagtgtga cgctttagg ggccttaga tttactcga 
      agctttaga tgtttgcgt tcagtgtga cattgaagg 
      ggagattga gtacgagtt tccaattta taatcatgt 
      ggagactct tgaagggtt gtgggattc ggaggatcc 
      gagatatgc gagcaaagg atcacagta tggtggttt 
      aaggttgta caccccacc agggacgta ccaccagtt 
      caaatcaat tttccccca cattcaagt tgagttgcg 
      gagagcaat tttggccaa ttatagcaa caatgatag 
      tgatatgga tttatacca atggcattt aggctcaaa 
      actatgtaa cacagaact cgaaagtct cattacgtt 
      ggaagaaga cgaagtcgc tttacaatt cattgagat 
      aaaaatcag gggaccaaa taattcctc gcaatactg 
      tttctactt tttggagca aataatgca aacgataaa 
      tcacgcttt attgagagt ttgtcccgg actgcttat 
      cgagaaaca cgtcttgag ccctttgga attgaagag 
      acactcaga ggggccttt ttgttaccc ctctgatat 
      catcacaac tgcaatgct tgaagctga gtcaactct 
      cgatgtcgc cttgtacaa ccccaatgt tgatgctca 
      agaaaccat cgcccagtt aattatgtt tagcgattt 
      ggatttggc gggtaacat ccagtgggg ctgcgccct 
      cagaatcca agacctcgc ccctccgat taaactgaa 
      gctgtgcgg tttgctgga ccccccgga gcggcacgg 
      tcgcgactg gtgcttgct agccctccg tttgggtct 
      ctgtcagga taaaatcgc cgctcttga ttcgcaaca 
      ttccagcca tacgatgag actgctaac aacagctga 
      ttgttcaat aggtgctct tataaccag tttgcatga 
      actagaccg actcgatgc cgctgaagt cgtcctgcg 
      ttccgcacc catatttaa aattataga gcatgtcag 
      tgtttagtc atagtgaac actcttctc tagctcaaa 
      attcttcaa cggtccaat gtctgttag ttagtgctt 
      ctaccgtcc cccctttta ttagtccaa tcaaacaac 
      gaagtagct acttatagt ttaatcagc tattacagg 
      gttcatttc agtattaac aaatgaagg agattgtga 
      tatttatgc tcagttgtg ggaaaatat tttattgta 
      taaataaat gtgatacaa gtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY