| General information | |||
| TC006299 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid54: 120719..134983 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006299 | ||
| General information | ||||
| iB_04105 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC006299 RA
MNTSLSRGC SYSPRESFE YTALQFAAR RGSLEQVRY LIESGTDLD TGPDPALHL ALRKQNTAI AHVLLHAGA DFEIKDSHG DYPIHIACT LGLLDVVHA LCALGCSVE VLTAKGLSP LHLAAKNGH IHVVRCLCA AGCNIDIRN SDNIRADIT ALKYGHNDI AELLDRLRV TGQRDTLAR QLVPTSKPA LRLNLRLLG HCGVGKTSL VKSLSAGLF GSLFRRSSS LQSNKSRPS SPINTQIEM DVTSRQNSL SFESSGNYQ STNGISVQN MDISNVGET TVWEFSGQE NYFPIYHHF LWPSPHSLT LVLFSLEDS PSIQVQQVC FWLNFLLAR QPADLPSSE YGQVMLVAT HVDLTRAVK TQNGEWICP DAQKTLETV RKIIPHVPN LMSSVVIMD TNVPASYPF KQLKSTLAE LKQECLQQT VGTWTGLLE ATLAWLNSL QKDYEQFPV LSRSTFSEL LRSQVNILA SDHHIQELL QQLHFMGEV FCVQDLVVV SVSWLGGQL LGELFSSDF FLHARVTGV YTSEDFQAS FNQCDALGA LDLLEALDV CVQCDIEGE IEYEFPIYN HVETLEGLW DSEDPRYAS KGSQYGGLR LYTPPGTYH QFKSIFPHI QVELRRAIL ANYSNNDSD MDLYQWHLG SKLCNTELE SLITLEEDE VALQFIEIK IRGPNNSSQ YCFYFLEQI MQTINHALL RVCPGLLIE KHVLSPLEL KRHSEGPFC YPSDIITTA MLEAESTLD VALYNPNVD AQETIAQLI MFSDLDLAG NIQWGCALR IQDLAPPIK LKLCGLLDP PERHGRDWC LLALRLGLC QDKIAALDS QHSSHTMRL LTTADCSIG ALITSLHEL DRLDAAEVV LRSAPIFKI IEHVSV
- >TC006299 RA
atgaacacc tccttaagc cggggctgt tcttacagc cctcgggag tccttcgaa tacactgcg cttcaattt gcagctcga agaggctct ttggagcaa gtccgatat ttgatcgag agtggcact gatcttgac acagggccg gaccctgcc ctacatcta gccctccgg aagcaaaac acggcgata gcacatgtg ctcctccat gctggggct gattttgag atcaaagat tcacatgga gattatccc atccatatt gcttgtact ttagggctt ttggacgtg gtccatgcc ctgtgtgcg ttaggatgt agtgtcgaa gttctgacg gcgaaaggg ctttcgccc ttgcatttg gcggcgaag aatggacat attcatgtt gtcaggtgt ttatgtgcc gctgggtgc aacatagac atccgtaat tctgataac atcagggct gatatcacg gctttgaaa tatggtcat aacgacatt gccgagttg ttggataga ttaagggtg actgggcaa agggacact ttagcccgt caattagta ccaacttcc aaaccagcc cttagatta aacttgcgg cttttgggg cactgtggt gtcgggaaa acttccctg gtcaagtcg ttaagtgcc ggtttgttt ggttcgtta ttccggcga tcgagttcg ctccaaagc aacaaatct cgtccttca tcccccatt aacacccaa atcgagatg gatgtcacc tcacgacaa aattctcta agttttgaa agcagtggc aattaccag tccacaaac ggcattagt gtacaaaat atggacata tctaacgtt ggcgaaaca acagtttgg gagttctca ggacaggaa aactatttt cccatctat caccacttt ttatggcct tcacctcat tcgcttact ttggttttg ttcagtttg gaagactcg ccgtccatt caagtgcaa caagtttgc ttctggctc aattttctt ctagcgagg caaccggct gacctgcct tcttccgaa tacggccaa gtcatgttg gtggccacc cacgtggac ttgacccgc gctgtgaag acgcaaaac ggcgaatgg atttgtccc gacgctcag aaaacgctc gaaactgtc cgaaaaata atcccccac gtccccaat ctcatgtcc agtgttgtg ataatggac acaaacgtg ccagcttcc taccctttc aagcaactc aagtcgacg ctggccgaa ctgaaacaa gagtgcctc cagcaaacc gtggggacc tggaccggc ctcctggaa gcaacccta gcttggctc aactcgctc cagaaggat tacgagcag tttcccgtt ttgtcgcgt tctacgttt tcggagttg ttgcgaagt caggtcaac atcctggct tcggaccac cacatccag gagttgctc cagcagttg cacttcatg ggggaggtg ttctgcgtc caggatttg gtcgtggtt tcggtttcc tggctaggg gggcaactt ctaggcgag cttttctca agcgatttt ttcctacat gcacgagtg actggcgtc tacactagt gaagatttc caagctagt ttcaaccag tgtgacgct ttaggggcc ttagattta ctcgaagct ttagatgtt tgcgttcag tgtgacatt gaaggggag attgagtac gagtttcca atttataat catgtggag actcttgaa gggttgtgg gattcggag gatccgaga tatgcgagc aaaggatca cagtatggt ggtttaagg ttgtacacc ccaccaggg acgtaccac cagttcaaa tcaattttc ccccacatt caagttgag ttgcggaga gcaattttg gccaattat agcaacaat gatagtgat atggattta taccaatgg catttaggc tcaaaacta tgtaacaca gaactcgaa agtctcatt acgttggaa gaagacgaa gtcgcttta caattcatt gagataaaa atcagggga ccaaataat tcctcgcaa tactgtttc tactttttg gagcaaata atgcaaacg ataaatcac gctttattg agagtttgt cccggactg cttatcgag aaacacgtc ttgagccct ttggaattg aagagacac tcagagggg cctttttgt tacccctct gatatcatc acaactgca atgcttgaa gctgagtca actctcgat gtcgccttg tacaacccc aatgttgat gctcaagaa accatcgcc cagttaatt atgtttagc gatttggat ttggcgggt aacatccag tggggctgc gccctcaga atccaagac ctcgcccct ccgattaaa ctgaagctg tgcggtttg ctggacccc ccggagcgg cacggtcgc gactggtgc ttgctagcc ctccgtttg ggtctctgt caggataaa atcgccgct cttgattcg caacattcc agccatacg atgagactg ctaacaaca gctgattgt tcaataggt gctcttata accagtttg catgaacta gaccgactc gatgccgct gaagtcgtc ctgcgttcc gcacccata tttaaaatt atagagcat gtcagtgtt tag
- >TC006299 RA
ttgttgcaa agtaaaaaa caccgcata cgggccatt taataagga acaatgcgc acaaaagga cctttgttg cacccaagt ggtttaatt catggaagt cagtttacg gggaacatc tgggacgat ttagtgacc ttcggacac attgttaaa aatttgtct ttgccgaaa gagtaattc ctaagaaat ttctaaacg ttatccgat ccccgatta aaactgtaa attcctccc acgttgtgt aaagacgag ccatcagcg tgggcactc ctatcacgt gtaattaca tctgaatat cctatttac gactccaca aatctcttt tgatttgat tacctcctc ttgacccgt aacgagaaa cctccaagc caaaaaatc tcgcatgca attattcca agctcacta atgcacagt tatgtagcc tccaaatcg tagagccag cctcgggac cgtttcgta tcagaaaca aaccatcgg agtggatat taacccaag tcgtttgca ttcatattg gcttaagga gatttcgta acataaatt gaacgccgt tcctggaag cgcttttaa ttccgtttg gcatttgaa tcttccaag aaaacaaat tacacgtta tttcacatt cgtacacac tggatgcat gtgtgcagc gcagctccg cggcatttc tcatttcaa tgcgttttt aaatcacgc gaccgattt ttcgattcg aaagacagc agcggttcc gagaaccga ttaatttcg atattgttt gagaattca tacaatcgt tacttgcga aaatcaagt ccggtttaa ttttagaaa cggactcct ttcggagaa gaaattgtg caaattaag cgccaagaa ttttccatt tgttctatc tttaacaaa tatttacgc gagtatagt ctaggtcag gttcaattt ctgataagc gattgcggc gccatcttg cgccttgac acgcctcca tttgcaggt tccttcgcc cgggaaagc cggaaaatt cggaaaatc tcaccatta aatcaaaca atcaactat ttcattctc agtttttta gactagtgt tgaaatcaa agaaatttt gcgaatttc ctttgtgac ttttccggc cgccggaaa ttatcgatc ggtgatcag ctgttgttt tccgcggtt gcagcacaa gtttccatg gaaaatgtg ataaaaaca ctgggctta gaatgcgtt tgaaggcca gtcccttag tttccctca gttttcctt taaaatgaa cacctcctt aagccgggg ctgttctta cagccctcg ggagtcctt cgaatacac tgcgcttca atttgcagc tcgaagagg ctctttgga gcaagtccg atatttgat cgagagtgg cactgatct tgacacagg gccggaccc tgccctaca tctagccct ccggaagca aaacacggc gatagcaca tgtgctcct ccatgctgg ggctgattt tgagatcaa agattcaca tggagatta tcccatcca tattgcttg tactttagg gcttttgga cgtggtcca tgccctgtg tgcgttagg atgtagtgt cgaagttct gacggcgaa agggctttc gcccttgca tttggcggc gaagaatgg acatattca tgttgtcag gtgtttatg tgccgctgg gtgcaacat agacatccg taattctga taacatcag ggctgatat cacggcttt gaaatatgg tcataacga cattgccga gttgttgga tagattaag ggtgactgg gcaaaggga cactttagc ccgtcaatt agtaccaac ttccaaacc agcccttag attaaactt gcggctttt ggggcactg tggtgtcgg gaaaacttc cctggtcaa gtcgttaag tgccggttt gtttggttc gttattccg gcgatcgag ttcgctcca aagcaacaa atctcgtcc ttcatcccc cattaacac ccaaatcga gatggatgt cacctcacg acaaaattc tctaagttt tgaaagcag tggcaatta ccagtccac aaacggcat tagtgtaca aaatatgga catatctaa cgttggcga aacaacagt ttgggagtt ctcaggaca ggaaaacta ttttcccat ctatcacca ctttttatg gccttcacc tcattcgct tactttggt tttgttcag tttggaaga ctcgccgtc cattcaagt gcaacaagt ttgcttctg gctcaattt tcttctagc gaggcaacc ggctgacct gccttcttc cgaatacgg ccaagtcat gttggtggc cacccacgt ggacttgac ccgcgctgt gaagacgca aaacggcga atggatttg tcccgacgc tcagaaaac gctcgaaac tgtccgaaa aataatccc ccacgtccc caatctcat gtccagtgt tgtgataat ggacacaaa cgtgccagc ttcctaccc tttcaagca actcaagtc gacgctggc cgaactgaa acaagagtg cctccagca aaccgtggg gacctggac cggcctcct ggaagcaac cctagcttg gctcaactc gctccagaa ggattacga gcagtttcc cgttttgtc gcgttctac gttttcgga gttgttgcg aagtcaggt caacatcct ggcttcgga ccaccacat ccaggagtt gctccagca gttgcactt catggggga ggtgttctg cgtccagga tttggtcgt ggtttcggt ttcctggct aggggggca acttctagg cgagctttt ctcaagcga ttttttcct acatgcacg agtgactgg cgtctacac tagtgaaga tttccaagc tagtttcaa ccagtgtga cgctttagg ggccttaga tttactcga agctttaga tgtttgcgt tcagtgtga cattgaagg ggagattga gtacgagtt tccaattta taatcatgt ggagactct tgaagggtt gtgggattc ggaggatcc gagatatgc gagcaaagg atcacagta tggtggttt aaggttgta caccccacc agggacgta ccaccagtt caaatcaat tttccccca cattcaagt tgagttgcg gagagcaat tttggccaa ttatagcaa caatgatag tgatatgga tttatacca atggcattt aggctcaaa actatgtaa cacagaact cgaaagtct cattacgtt ggaagaaga cgaagtcgc tttacaatt cattgagat aaaaatcag gggaccaaa taattcctc gcaatactg tttctactt tttggagca aataatgca aacgataaa tcacgcttt attgagagt ttgtcccgg actgcttat cgagaaaca cgtcttgag ccctttgga attgaagag acactcaga ggggccttt ttgttaccc ctctgatat catcacaac tgcaatgct tgaagctga gtcaactct cgatgtcgc cttgtacaa ccccaatgt tgatgctca agaaaccat cgcccagtt aattatgtt tagcgattt ggatttggc gggtaacat ccagtgggg ctgcgccct cagaatcca agacctcgc ccctccgat taaactgaa gctgtgcgg tttgctgga ccccccgga gcggcacgg tcgcgactg gtgcttgct agccctccg tttgggtct ctgtcagga taaaatcgc cgctcttga ttcgcaaca ttccagcca tacgatgag actgctaac aacagctga ttgttcaat aggtgctct tataaccag tttgcatga actagaccg actcgatgc cgctgaagt cgtcctgcg ttccgcacc catatttaa aattataga gcatgtcag tgtttagtc atagtgaac actcttctc tagctcaaa attcttcaa cggtccaat gtctgttag ttagtgctt ctaccgtcc cccctttta ttagtccaa tcaaacaac gaagtagct acttatagt ttaatcagc tattacagg gttcatttc agtattaac aaatgaagg agattgtga tatttatgc tcagttgtg ggaaaatat tttattgta taaataaat gtgatacaa gtta