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Gene information
General information
TC006290 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6556793..6581239
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006290
    iBeetle screen iB_04103

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    gnathal appendages mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    prothorax partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: animals lost,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006290 RA
      MTWGFVTCG PNEALVISG CCYSKPLLV PGGRAFIWP 
      TIQRIQRIC LNTMTLIVD SPTVYTSQG VPISVTGIA 
      QVKIQGQNE EMLLAACEQ FLGKTEEEI QHIALVTLE 
      GHQRAIMGS MTVEEIYKD RKKFSKQVF EVASSDLVN 
      MGITVVSYT LKDIRDEEG YLKSLGMAR TAEVKRDAR 
      IGEAEARAD AQIKAAIAE EQRMASVFL NDTEIAKAK 
      RDFELKKAA YDVEVQTKN AEAELAYEL QAAKTKQKI 
      KEEQMQILV VERTQQIAV QDQEMQRRE KELEATVRR 
      PAEAEKYKL EKLAEADHN RIILEAQAQ AEAVRLKGE 
      AEAFAIEAK AKAEAEQMA KKADAFKEY KEAAMIDMF 
      LDVLPKVAA EVAAPISQT KKITMVSTG SGEIGAAKL 
      TGEVLDIVN KVPQLVKNL TGVDIAKNI HAA
    CDS sequence
    • >TC006290 RA
      atgacgtgg ggatttgtc acatgcggc cccaacgaa 
      gcactagtc atctcagga tgttgttac agcaaaccg 
      cttttggta cccggaggt agggctttt atctggcca 
      acgattcag aggatacaa agaatatgc ctgaacacg 
      atgacgcta attgtggac agtccgacg gtttacacg 
      agtcagggg gttcccatc tcggtgacg gggatcgct 
      caggtgaag attcagggc caaaacgag gagatgtta 
      ttggccgct tgcgagcag tttttgggc aaaacggaa 
      gaagaaatc cagcacatt gctctagtg actctagaa 
      ggccaccag agggccatc atgggctcc atgaccgtc 
      gaagaaatc tacaaagac cgcaaaaaa ttcagcaaa 
      caagtgttc gaagtggcg tcttctgac ttggttaat 
      atgggaatt acggtcgtt tcgtacact ttgaaggac 
      atcagggac gaagagggc tacctaaaa agtcttggc 
      atggctcga accgctgaa gtaaaacgc gacgcccga 
      atcggcgaa gccgaagcc cgggccgac gcccaaatc 
      aaggctgcc atcgccgaa gagcaacgc atggcttcg 
      gtgttcctc aacgacacc gaaatcgcc aaagccaag 
      cgtgatttc gagctgaaa aaagccgct tacgacgtc 
      gaagtccaa acgaagaac gccgaagcc gagctggct 
      tacgaactg caagcggcc aagacaaag cagaagatc 
      aaggaggag cagatgcag attttggtg gtggagagg 
      acgcagcag attgccgtc caagaccag gagatgcag 
      aggagggag aaggagttg gaggcgacg gtgaggagg 
      ccggcggaa gcggaaaag tacaaattg gagaagttg 
      gctgaggct gatcataat aggattatt ttggaagcg 
      caagcgcaa gctgaggct gtgaggctt aaaggggaa 
      gccgaggcg tttgctatt gaggccaag gcgaaagcc 
      gaggccgag caaatggcc aagaaggcg gatgcgttt 
      aaggagtac aaggaggcg gcgatgatt gatatgttc 
      ttggatgtt ttaccaaag gttgctgcc gaagtggcg 
      gcccctata tcccaaacc aagaaaatc acgatggtc 
      tcgacaggc tcgggtgaa atcggcgct gctaagcta 
      acgggggaa gtactcgat attgtcaac aaggtccct 
      cagctcgtt aagaattta accggtgtt gatatagcc 
      aagaatatt cacgccgca tag
    mRNA sequence
    • >TC006290 RA
      ctaaagccg attctgtca acacgaatg aatttcgcc 
      tcgaaaagt gtgtgtttg tgtgcttag acaccaatt 
      tccgcatat cagcgctaa acatgacgt ggggatttg 
      tcacatgcg gccccaacg aagcactag tcatctcag 
      gatgttgtt acagcaaac cgcttttgg tacccggag 
      gtagggctt ttatctggc caacgattc agaggatac 
      aaagaatat gcctgaaca cgatgacgc taattgtgg 
      acagtccga cggtttaca cgagtcagg gggttccca 
      tctcggtga cggggatcg ctcaggtga agattcagg 
      gccaaaacg aggagatgt tattggccg cttgcgagc 
      agtttttgg gcaaaacgg aagaagaaa tccagcaca 
      ttgctctag tgactctag aaggccacc agagggcca 
      tcatgggct ccatgaccg tcgaagaaa tctacaaag 
      accgcaaaa aattcagca aacaagtgt tcgaagtgg 
      cgtcttctg acttggtta atatgggaa ttacggtcg 
      tttcgtaca ctttgaagg acatcaggg acgaagagg 
      gctacctaa aaagtcttg gcatggctc gaaccgctg 
      aagtaaaac gcgacgccc gaatcggcg aagccgaag 
      cccgggccg acgcccaaa tcaaggctg ccatcgccg 
      aagagcaac gcatggctt cggtgttcc tcaacgaca 
      ccgaaatcg ccaaagcca agcgtgatt tcgagctga 
      aaaaagccg cttacgacg tcgaagtcc aaacgaaga 
      acgccgaag ccgagctgg cttacgaac tgcaagcgg 
      ccaagacaa agcagaaga tcaaggagg agcagatgc 
      agattttgg tggtggaga ggacgcagc agattgccg 
      tccaagacc aggagatgc agaggaggg agaaggagt 
      tggaggcga cggtgagga ggccggcgg aagcggaaa 
      agtacaaat tggagaagt tggctgagg ctgatcata 
      ataggatta ttttggaag cgcaagcgc aagctgagg 
      ctgtgaggc ttaaagggg aagccgagg cgtttgcta 
      ttgaggcca aggcgaaag ccgaggccg agcaaatgg 
      ccaagaagg cggatgcgt ttaaggagt acaaggagg 
      cggcgatga ttgatatgt tcttggatg ttttaccaa 
      aggttgctg ccgaagtgg cggccccta tatcccaaa 
      ccaagaaaa tcacgatgg tctcgacag gctcgggtg 
      aaatcggcg ctgctaagc taacggggg aagtactcg 
      atattgtca acaaggtcc ctcagctcg ttaagaatt 
      taaccggtg ttgatatag ccaagaata ttcacgccg 
      cataggaca acgatatta agggatcga ggaccaatt 
      ttcgcattt aacaacacc ttgcagccg ctttcggat 
      ggagatgac tatcgtagc ttaaatcct cactcaaat 
      cgagcctta taaatgtgt aaatgtata ttttttgta 
      tgccatatg tatgtgatt tttggtgtg taccttgta 
      tatgtacag aggagaggt ctggctcac gagcgatgc 
      ctagaatca ccccacttt tatgtaatt actaaactt 
      gttgcggat tcttcaagc aaattttcc ctcattatt 
      aattgaaga tattcgaat tgtgttgtt cacgcgtgt 
      ttgttttgt tgtagcacg agttttacc aatcatggg 
      ttattatag cattgatgt ggaagtcct tgttgttat 
      tatatgatg ccaaaaatt tattcccaa agtcactgt 
      aaattctgg cttgtacga ataaacgat attttacta 
      t
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