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Gene information
General information
TC006286 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid53: 363028..364254
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006286
    iBeetle screen iB_04102
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006286 RA
      MQQAFLELN GVRTNVLTY GKWVEESFK PTEARDIIL 
      VIPGNPGLT GFYKMFMHT LNEKTGFPV WIVGHAGHE 
      LPKKNSLFS VQPLQGNEG LFGLRGQVE HKLDFIKKY 
      VPEDARLHL VGHSIGSYM ILELLKEEL VRNKVVDVK 
      LLFPTFEYM AETANGKFL NFIKPIIWL VVFLSWIYT 
      ILPSILSNF LMYLYMKIM CIPKMHFRT MVHLIEPSI 
      LEKVFFLAY EELQNVRER NDTLIKENC NKIKFLYGH 
      KDGWAPPTY CDNLKKDIP GVNTEMSHF DHSFVLKQS 
      REVGSVVAG WIC
    CDS sequence
    • >TC006286 RA
      atgcagcaa gcctttctt gaattaaat ggtgttaga 
      accaacgtt ttgacctat gggaaatgg gtggaagag 
      tcgtttaaa ccaacggag gcccgagac attattttg 
      gtgattccg gggaatcct ggattgaca gggttttac 
      aaaatgttt atgcacacc ttgaatgaa aaaactggc 
      tttccagtg tggattgtg ggccacgct gggcatgaa 
      ctaccgaag aagaattca cttttttca gtgcaaccg 
      ttgcaaggg aatgagggg ttgtttggg ttgagggga 
      caagtggag cacaagctt gattttatt aaaaaatac 
      gtcccggaa gacgcaaga ctccaccta gtcgggcac 
      tcaattggc tcttacatg attctggag ctacttaag 
      gaagaatta gtgagaaat aaagtcgtt gatgttaaa 
      ttactgttc ccaacgttc gaatacatg gcagagaca 
      gctaacgga aagttttta aatttcatt aaaccaatc 
      atttggttg gtcgttttc ctgtcgtgg atttacacg 
      atcttgccc tcgatttta tcgaacttt ttaatgtat 
      ctttacatg aagataatg tgtataccg aagatgcat 
      tttaggaca atggttcac ttaatcgag cctagtatt 
      ttagaaaaa gtatttttc ctggcctat gaggaactc 
      caaaacgtt agagaacga aacgatacg cttattaag 
      gaaaactgc aataaaatc aaattcttg tatgggcat 
      aaagacgga tgggctcct ccaacttac tgtgataac 
      ttaaaaaaa gacattccg ggagtcaac accgagatg 
      agccacttc gaccactct ttcgtccta aagcaaagt 
      cgcgaagtt ggcagtgtt gtagctggt tggatttgt 
      taa
    mRNA sequence
    • >TC006286 RA
      aaataaaca aatgaccat tagttaggc aacaagtta 
      tgcactcaa ctgacctca actgtgatg acctcaaat 
      caaaaaaca caatttgtg taaaaacct gcttttaca 
      acaatgcga gctagttgt tgtttggct gtaaacaat 
      tggaaaaca ttttagtta atcggctta tcgattaga 
      gtgtggagg ttaggggga gaccttaaa ctttgtacc 
      aaattaaaa agtgtgtct tgcgtgacc gtggaaaat 
      gcagcaagc ctttcttga attaaatgg tgttagaac 
      caacgtttt gacctatgg gaaatgggt ggaagagtc 
      gtttaaacc aacggaggc ccgagacat tattttggt 
      gattccggg gaatcctgg attgacagg gttttacaa 
      aatgtttat gcacacctt gaatgaaaa aactggctt 
      tccagtgtg gattgtggg ccacgctgg gcatgaact 
      accgaagaa gaattcact tttttcagt gcaaccgtt 
      gcaagggaa tgaggggtt gtttgggtt gaggggaca 
      agtggagca caagcttga ttttattaa aaaatacgt 
      cccggaaga cgcaagact ccacctagt cgggcactc 
      aattggctc ttacatgat tctggagct acttaagga 
      agaattagt gagaaataa agtcgttga tgttaaatt 
      actgttccc aacgttcga atacatggc agagacagc 
      taacggaaa gtttttaaa tttcattaa accaatcat 
      ttggttggt cgttttcct gtcgtggat ttacacgat 
      cttgccctc gattttatc gaacttttt aatgtatct 
      ttacatgaa gataatgtg tataccgaa gatgcattt 
      taggacaat ggttcactt aatcgagcc tagtatttt 
      agaaaaagt atttttcct ggcctatga ggaactcca 
      aaacgttag agaacgaaa cgatacgct tattaagga 
      aaactgcaa taaaatcaa attcttgta tgggcataa 
      agacggatg ggctcctcc aacttactg tgataactt 
      aaaaaaaga cattccggg agtcaacac cgagatgag 
      ccacttcga ccactcttt cgtcctaaa gcaaagtcg 
      cgaagttgg cagtgttgt agctggttg gatttgtta 
      attttttat tgaataaaa ggttttggt tcaa