Status
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Gene information
General information
TC006285 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6224390..6237014
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006285
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04101

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006285 RA
      MNWVTHCST DSADYQVFN LKGSSIKLA KWQTEPFTE 
      RVLQQLNDE PKVILSSVK NNKRNVQRT DHNVPDLCD 
      IGNYVECST HEDIIYTSE KCVKLPDDG GFFFDIEEK 
      TDNAKKHDV EKELSIMFQ NVTTEEETA IKKPSHRRR 
      QHKPKKTKN LRELLIQLF GSPLDDIEK PPKTPQKVQ 
      NNRRQSLPV SIQIAGADF FKPVKINNK RKSEADIPS 
      CKKVQKTVQ VPVLEVFNV DYLSVAELK IPPIYSETA 
      TSSVTLEEL KVATVLYKP RLSKTQTVT NTHIIDLTQ 
      DSSNSFSST DIPVEIKGE QANVGLSMP SLSPLEEGA 
      KSRPETTSV QPPKTVEKM VATTQRRQS VDNVQSQPN 
      TRIQINRQQ SAPQLQQAE RPIRAQQTA LEQLMEWRK 
      NQIQPPSQP HWISNEHNM PQLLRIHQN TPSGYLLIS 
      NRVCGEKIF PYVYKYYVW KQKLVQLMD LKKSLFSAD 
      EVKENLETL ASYYEATRR NIAKRLIKE LLPLEIFNF 
      VLSDVLSTI STKMVEKQL DGGLCRVCC LIHNIMQGF 
      EDACITPFT KRFYSVVSA SMGRLDERS RHVFTSQQL 
      KLLELQITQ FHLIQCWIN ARAHAANQR PKSPKTNKR 
      KTTVIQSNP AKRNERTIV PRILVHSKE NVQSTLTPK 
      PPELPPYRS YNQGPLVNV SRPQETNNV TFRMPQETS 
      IFAFSRPQI PILPRPPPP QQVAETQQT NIVPRTPET 
      NVSTSFTRP QELTLRSLL NSQARPQEA NTEQGWSKV 
      PERVPTVQS TETKEPQTA EATAEEARK DSDDVIDLT 
      WIDDADEKA IYDEIIECK VFEFKDEFK NDNNAGETS 
      DREVVSPAD SGLGSPVEQ TDDGFKCLC GNRAIYVCA 
      CKSSMYCSQ ECQVNDWTY HQKMCNKTK S
    CDS sequence
    • >TC006285 RA
      atgaattgg gtgacgcat tgcagcact gattccgct 
      gattaccaa gtatttaat ctgaaagga agctcaata 
      aaactggcc aagtggcag actgaacca tttactgaa 
      agagtatta caacagttg aacgacgaa ccgaaagtg 
      atactgtca tcggtgaaa aataacaaa agaaacgtc 
      caacggacg gatcacaat gtaccagat ttgtgtgac 
      attggaaac tacgttgaa tgctcaacc cacgaagat 
      attatttat actagcgaa aaatgtgtg aagttgcca 
      gacgatggt ggttttttc ttcgacatt gaggaaaaa 
      acagacaat gcgaaaaaa cacgacgtc gaaaaggaa 
      cttagtata atgttccaa aatgtcacc acagaagaa 
      gaaacagca ataaaaaaa ccgtcacat cgtcgtcga 
      caacataaa cccaagaaa acgaaaaac ttgcgtgaa 
      ctgctcatc caactattc ggaagtcca ttggatgac 
      atcgaaaaa ccaccaaaa acgccccaa aaagtgcaa 
      aacaatcga agacaatct ctgcccgtt tcgatccaa 
      attgcgggt gcggatttt ttcaaaccg gtgaaaatt 
      aacaataaa cgtaaaagt gaagcagac attccctcg 
      tgtaaaaaa gtgcaaaaa acggtgcaa gttccagtg 
      ttggaggta ttcaacgtt gattacctc agtgttgcg 
      gagttgaaa ataccacca atatactca gaaactgcc 
      actagtagt gtaacactt gaagaactg aaagtggcc 
      actgtttta tacaaacct cgtttgagc aaaactcaa 
      accgtgacc aatacgcac atcattgat ctgactcag 
      gatagtagc aacagtttt agttcgact gatatacct 
      gtggaaatt aaaggcgaa caagccaat gttggtttg 
      tctatgccg tctttatca ccacttgag gaaggagcg 
      aaatctcgt ccggaaacc acatccgtt caaccgcca 
      aaaaccgta gaaaaaatg gtggctact acgcagcgt 
      aggcaaagt gttgacaat gtgcaatcg cagccgaac 
      actcggatt caaatcaat cggcaacag agtgctcca 
      caactgcag caagccgag agaccaata agagcgcaa 
      cagacggcg ttagagcag ttgatggag tggaggaag 
      aatcaaata caaccgccg agtcaaccg cattggatt 
      tccaacgag cataatatg ccacaattg ttgcgaatt 
      caccaaaac acaccgagt ggttatctc ctcatttcg 
      aatagggtg tgtggggaa aaaatcttt ccatatgtt 
      tacaagtat tacgtatgg aaacaaaaa ctagtccag 
      ttgatggac ttgaagaag agtctcttt tccgctgat 
      gaagtgaaa gagaatctc gagactctt gctagttac 
      tatgaggcg accagacgg aatattgcc aaaagattg 
      atcaaagag ttattacca ctggagata tttaatttt 
      gtgcttagt gatgtcttg agtacgata tcgactaaa 
      atggtcgag aagcagtta gatgggggt ctgtgccgt 
      gtttgttgt ttaatccat aacatcatg caggggttt 
      gaggacgct tgcattacc ccgtttacc aagaggttc 
      tatagcgtt gttagtgct tcaatggga cgactcgat 
      gaacgatcc cggcatgtg tttacaagc caacaattg 
      aaactactg gaactgcaa ataacgcag ttccatttg 
      attcagtgt tggattaac gcccgagca catgccgcc 
      aaccaaaga cccaaatca cccaaaacc aataagagg 
      aaaacgact gtgatacag agcaatcca gcgaaaaga 
      aacgaaaga actattgta cctagaatt ctggtacat 
      agcaaggaa aatgtgcaa tctactctt acgccaaaa 
      ccaccagag cttcctccg tataggagc tataaccaa 
      gggccactg gtgaatgtt tcaaggcca caagagact 
      aacaatgtg acatttcgt atgccacaa gagactagc 
      atttttgcg ttctcaaga cctcaaata ccaatcctt 
      ccaaggccg ccgccacca caacaagtg gccgaaaca 
      cagcaaact aatattgtt ccaaggacg ccagaaacg 
      aacgtttcg acgtcgttc acaaggcca caagaattg 
      actcttagg tcgttgttg aattcgcaa gcaagacct 
      caagaagct aatactgaa caaggatgg agtaaagta 
      cctgaacga gtccctaca gtgcaatca acggaaacc 
      aaagaaccg caaacagct gaagcgacg gcagaagaa 
      gcgaggaaa gatagcgac gatgtgatt gatctgaca 
      tggattgac gatgcggat gagaaggct atttacgat 
      gagataatt gagtgtaaa gtgtttgag ttcaaagat 
      gagtttaaa aatgacaac aatgcggga gagacgtcg 
      gatcgagaa gttgtttca ccggccgat agtgggtta 
      gggagtccg gttgagcaa acggacgat gggttcaaa 
      tgtctctgt gggaacaga gccatttat gtatgcgct 
      tgcaagtcg tcaatgtat tgcagtcaa gagtgtcaa 
      gttaacgac tggacgtac caccaaaaa atgtgtaat 
      aaaacaaag tcctaa
    mRNA sequence
    • >TC006285 RA
      aatctacaa taaacaaaa gccccagct ttgctttaa 
      actctgatt taaataaaa acagccggt accggtaaa 
      aaaccggcc cccaactca ataagagat tatttccac 
      gtaaacgca aagaatgtc gctttatta cttgccgaa 
      aaatgggca aaaacagtc aaagtgttt agttgcaaa 
      aaatatcaa gttgccgtg accacattg caattgttt 
      tttggaagt ttttcaatt ctgttgttc caccttttt 
      aaattaaaa aaacctcaa ttccgtgat tattcaaca 
      ggtatgtgc tatattttg gtacttttt aaccgatca 
      cagttgata aacttgaat tagttgcag tttttatgt 
      ttttggggg ttgtttaaa ttttgcgaa ttgttaggt 
      taggaacag gcccttgcg ggggcctat ttcagtgat 
      ttgagactt gaagtcgaa taaactgac ccacaatgt 
      gttttttgc gttcgatgc agtttcttt catgacaaa 
      atctgcaaa cgtaatctg aagttacaa tcgacagtt 
      tgcagttct tcgaaatga ataatgtgc gtgcctgat 
      ttcccagtt cttagaacg cctaaccgg ttttaaatc 
      taactaaca agggtgctt ctgagaact gacaattct 
      ttgccccac tacctgaag ttttgtgag cttaacagt 
      gccctaaag tcaccttga agtctgaaa taaatttag 
      ttgacgcct cttctcacg acctgtcta gactagatc 
      ggtcccaca agacggtaa gtgaaatac tgtgctcag 
      tgtaaaact tttctcgac tttaatatt ttgtaaaat 
      ttttttcag gaaaagttt ggtctagct accagttat 
      tttgttatt tttataact acttattta ggtctggtg 
      atgtcagca gttgaataa tgatttccg actctgcta 
      tgtgagtta ataaaaaat gaattgggt gacgcattg 
      cagcactga ttccgctga ttaccaagt atttaatct 
      gaaaggaag ctcaataaa actggccaa gtggcagac 
      tgaaccatt tactgaaag agtattaca acagttgaa 
      cgacgaacc gaaagtgat actgtcatc ggtgaaaaa 
      taacaaaag aaacgtcca acggacgga tcacaatgt 
      accagattt gtgtgacat tggaaacta cgttgaatg 
      ctcaaccca cgaagatat tatttatac tagcgaaaa 
      atgtgtgaa gttgccaga cgatggtgg ttttttctt 
      cgacattga ggaaaaaac agacaatgc gaaaaaaca 
      cgacgtcga aaaggaact tagtataat gttccaaaa 
      tgtcaccac agaagaaga aacagcaat aaaaaaacc 
      gtcacatcg tcgtcgaca acataaacc caagaaaac 
      gaaaaactt gcgtgaact gctcatcca actattcgg 
      aagtccatt ggatgacat cgaaaaacc accaaaaac 
      gccccaaaa agtgcaaaa caatcgaag acaatctct 
      gcccgtttc gatccaaat tgcgggtgc ggatttttt 
      caaaccggt gaaaattaa caataaacg taaaagtga 
      agcagacat tccctcgtg taaaaaagt gcaaaaaac 
      ggtgcaagt tccagtgtt ggaggtatt caacgttga 
      ttacctcag tgttgcgga gttgaaaat accaccaat 
      atactcaga aactgccac tagtagtgt aacacttga 
      agaactgaa agtggccac tgttttata caaacctcg 
      tttgagcaa aactcaaac cgtgaccaa tacgcacat 
      cattgatct gactcagga tagtagcaa cagttttag 
      ttcgactga tatacctgt ggaaattaa aggcgaaca 
      agccaatgt tggtttgtc tatgccgtc tttatcacc 
      acttgagga aggagcgaa atctcgtcc ggaaaccac 
      atccgttca accgccaaa aaccgtaga aaaaatggt 
      ggctactac gcagcgtag gcaaagtgt tgacaatgt 
      gcaatcgca gccgaacac tcggattca aatcaatcg 
      gcaacagag tgctccaca actgcagca agccgagag 
      accaataag agcgcaaca gacggcgtt agagcagtt 
      gatggagtg gaggaagaa tcaaataca accgccgag 
      tcaaccgca ttggatttc caacgagca taatatgcc 
      acaattgtt gcgaattca ccaaaacac accgagtgg 
      ttatctcct catttcgaa tagggtgtg tggggaaaa 
      aatctttcc atatgttta caagtatta cgtatggaa 
      acaaaaact agtccagtt gatggactt gaagaagag 
      tctcttttc cgctgatga agtgaaaga gaatctcga 
      gactcttgc tagttacta tgaggcgac cagacggaa 
      tattgccaa aagattgat caaagagtt attaccact 
      ggagatatt taattttgt gcttagtga tgtcttgag 
      tacgatatc gactaaaat ggtcgagaa gcagttaga 
      tgggggtct gtgccgtgt ttgttgttt aatccataa 
      catcatgca ggggtttga ggacgcttg cattacccc 
      gtttaccaa gaggttcta tagcgttgt tagtgcttc 
      aatgggacg actcgatga acgatcccg gcatgtgtt 
      tacaagcca acaattgaa actactgga actgcaaat 
      aacgcagtt ccatttgat tcagtgttg gattaacgc 
      ccgagcaca tgccgccaa ccaaagacc caaatcacc 
      caaaaccaa taagaggaa aacgactgt gatacagag 
      caatccagc gaaaagaaa cgaaagaac tattgtacc 
      tagaattct ggtacatag caaggaaaa tgtgcaatc 
      tactcttac gccaaaacc accagagct tcctccgta 
      taggagcta taaccaagg gccactggt gaatgtttc 
      aaggccaca agagactaa caatgtgac atttcgtat 
      gccacaaga gactagcat ttttgcgtt ctcaagacc 
      tcaaatacc aatccttcc aaggccgcc gccaccaca 
      acaagtggc cgaaacaca gcaaactaa tattgttcc 
      aaggacgcc agaaacgaa cgtttcgac gtcgttcac 
      aaggccaca agaattgac tcttaggtc gttgttgaa 
      ttcgcaagc aagacctca agaagctaa tactgaaca 
      aggatggag taaagtacc tgaacgagt ccctacagt 
      gcaatcaac ggaaaccaa agaaccgca aacagctga 
      agcgacggc agaagaagc gaggaaaga tagcgacga 
      tgtgattga tctgacatg gattgacga tgcggatga 
      gaaggctat ttacgatga gataattga gtgtaaagt 
      gtttgagtt caaagatga gtttaaaaa tgacaacaa 
      tgcgggaga gacgtcgga tcgagaagt tgtttcacc 
      ggccgatag tgggttagg gagtccggt tgagcaaac 
      ggacgatgg gttcaaatg tctctgtgg gaacagagc 
      catttatgt atgcgcttg caagtcgtc aatgtattg 
      cagtcaaga gtgtcaagt taacgactg gacgtacca 
      ccaaaaaat gtgtaataa aacaaagtc ctaaaatga 
      agcgttgtt aagatcagt attaagttg ttatttcat 
      tttttattg tttcaagat tatctaaat tgtatttaa 
      tcttgttga atattagtt tagtatttt ttcttattt 
      ttgtaattt ccatgcatt accaaagtt gaataaagt 
      tgttttgat att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY