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Gene information
General information
TC006284 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6199612..6206930
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006284
    iBeetle screen iB_04100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: about half of the remnants appear inside out
    head posterior ventralnot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax anterior not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs potentiallyfused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen bristle segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006284 RA
      MIISFRKPT VKYFVIALT AAIILAILG TIVYFLVKG 
      ESSGKGAVV TNGHTCAHI GAAILAKGG SAVDAAIAA 
      LFCEGVSMP QSMGLGGGF LMTVYFAEN RTAYSLNAR 
      EVAPLAASK DMFDGNSSL STKGGLAVA VPGELRGYW 
      EAYHRYGGK VAWADLVQP TIDLCESGV LVTEYLEKL 
      YASQLESFK KDPVMAETF IDPSTNRTY KKGQVVKRP 
      RLAKSLELM KSFGADALY DGTLTKGFV EDIRNKGGI 
      ITVEDMKNY KVKWQEPIV VQMPNNQTL YTAPLPGSG 
      VILSFIMNL LNNYLDNSQ PLSVTNWQR IVESFKFGY 
      GKRTELGDI TDSNFANVT DLVKKLISP DYASEIRQK 
      IHDGTTSQD PSYYGAKTV QPEDHGTAH ICVLAPNGD 
      AVSVTSTIN LYFGAGFMS ESTGIILNN EMDDFSSPN 
      MNNSFNVPP SEANYIEPG KRPLSSMVP SIVVGDGGV 
      ELVIGAAGG TKITTSVAQ IAVKHLWFG APIGAAVQE 
      KRIHHQLFP MEIEFENAF NETRDFVEA LHKIGHEYT 
      IAEPGSGFS AVTAISRKG GDVATASDV RRTGDEVIV
    CDS sequence
    • >TC006284 RA
      atgattatt tccttcaga aaacccacc gtcaagtac 
      ttcgtcatc gctttgacc gccgccatc atcctggca 
      atcctcggc actattgtg tatttcctg gttaagggc 
      gagtcgagc ggcaagggg gctgtggtc accaacggg 
      cacacgtgc gcccacatc ggcgccgcc atcctcgcg 
      aaagggggc tcggcggtg gacgccgcc atcgcagct 
      ttgttctgc gagggcgtc tccatgccc cagagcatg 
      gggctgggc gggggcttc ctgatgacg gtctacttc 
      gccgagaac cgcacggcg tactcgctg aatgcgcgg 
      gaggttgcc ccgctggcg gcctccaaa gacatgttc 
      gatgggaac agctcgctg tcgacgaaa ggggggctt 
      gcggtggcg gtgcccggg gagttgagg ggctactgg 
      gaggcctac cacaggtat gggggcaag gtggcgtgg 
      gccgatttg gtgcagcct accatagat ttgtgcgaa 
      agtggggtc ctggtgacg gagtatttg gagaagttg 
      tatgcgagt cagttggaa agcttcaag aaggatcct 
      gtgatggct gagactttt atcgatccg agcactaat 
      cgcacctat aagaaggga caagtggtt aagaggcca 
      cggttggcc aaaagtttg gaactgatg aagagtttt 
      ggggccgat gctttgtat gacggtact ttgacgaag 
      gggtttgtt gaggatata cggaacaaa ggtgggatt 
      atcacggtg gaggatatg aaaaactac aaggttaaa 
      tggcaagaa cccatcgtg gttcaaatg cccaacaac 
      cagacgctt tacacggca cctttgccc ggctctggt 
      gtgatcctt agtttcata atgaacctc cttaacaat 
      tacttggac aattcgcaa cccttgtcc gtcacaaac 
      tggcaacga atcgtcgaa agcttcaaa ttcggctac 
      ggcaaaagg acagaactc ggtgacatc accgacagc 
      aactttgcc aatgtcaca gacttggtc aaaaagctc 
      atttcgccg gattatgcc tccgaaatt cggcaaaaa 
      atccacgac ggtaccacc tcccaagac ccgtcgtac 
      tacggcgct aagacggtc caacccgag gaccatggt 
      accgcccat atctgcgtc ctggccccc aatggggac 
      gcagtctcc gtcacaagc accatcaat ttatatttc 
      ggcgccggt ttcatgtcc gaaagcacc ggaatcatc 
      ctcaacaac gaaatggac gacttctcc tcccccaac 
      atgaacaac agcttcaac gtgccgccg tccgaagcc 
      aactacatc gagcccggg aagcgcccg ttgtcctcc 
      atggtgccc tcgattgtc gtgggggat ggcggggtt 
      gagctcgtg ataggggcc gcaggaggg accaaaatc 
      acgacctcg gtggcgcag atcgccgtc aagcatttg 
      tggtttggg gctccgatc ggagcagca gtgcaggag 
      aagcggatt caccatcag ttgttcccg atggagatt 
      gagttcgag aatgcgttt aatgaaacg agagatttt 
      gtggaagcg ttgcataaa attgggcat gagtatacg 
      atagctgag ccggggtcg gggttttcg gcggtgacg 
      gccatctcc cggaaaggg ggcgatgtg gcgacggcg 
      agtgatgtg cggaggacg ggcgacgaa gttatagtt 
      tga
    mRNA sequence
    • >TC006284 RA
      tctcgattc aaataatcc gattaaata aaacgcact 
      tgaagttat ctcgcggca tttaattat tatcattac 
      ctcaaatta ttatcattt taattacgc tcaagagcg 
      gtaagagcc tcagtttgt ccgccggat tcgtcgcgg 
      aaaagtgtt gcgtcgtac gaattaatt taatcaaac 
      tatttttac taccgtgtt tatttcact attaccgcc 
      tgataaccg caatgatta tttccttca gaaaaccca 
      ccgtcaagt acttcgtca tcgctttga ccgccgcca 
      tcatcctgg caatcctcg gcactattg tgtatttcc 
      tggttaagg gcgagtcga gcggcaagg gggctgtgg 
      tcaccaacg ggcacacgt gcgcccaca tcggcgccg 
      ccatcctcg cgaaagggg gctcggcgg tggacgccg 
      ccatcgcag ctttgttct gcgagggcg tctccatgc 
      cccagagca tggggctgg gcgggggct tcctgatga 
      cggtctact tcgccgaga accgcacgg cgtactcgc 
      tgaatgcgc gggaggttg ccccgctgg cggcctcca 
      aagacatgt tcgatggga acagctcgc tgtcgacga 
      aaggggggc ttgcggtgg cggtgcccg gggagttga 
      ggggctact gggaggcct accacaggt atgggggca 
      aggtggcgt gggccgatt tggtgcagc ctaccatag 
      atttgtgcg aaagtgggg tcctggtga cggagtatt 
      tggagaagt tgtatgcga gtcagttgg aaagcttca 
      agaaggatc ctgtgatgg ctgagactt ttatcgatc 
      cgagcacta atcgcacct ataagaagg gacaagtgg 
      ttaagaggc cacggttgg ccaaaagtt tggaactga 
      tgaagagtt ttggggccg atgctttgt atgacggta 
      ctttgacga aggggtttg ttgaggata tacggaaca 
      aaggtggga ttatcacgg tggaggata tgaaaaact 
      acaaggtta aatggcaag aacccatcg tggttcaaa 
      tgcccaaca accagacgc tttacacgg cacctttgc 
      ccggctctg gtgtgatcc ttagtttca taatgaacc 
      tccttaaca attacttgg acaattcgc aacccttgt 
      ccgtcacaa actggcaac gaatcgtcg aaagcttca 
      aattcggct acggcaaaa ggacagaac tcggtgaca 
      tcaccgaca gcaactttg ccaatgtca cagacttgg 
      tcaaaaagc tcatttcgc cggattatg cctccgaaa 
      ttcggcaaa aaatccacg acggtacca cctcccaag 
      acccgtcgt actacggcg ctaagacgg tccaacccg 
      aggaccatg gtaccgccc atatctgcg tcctggccc 
      ccaatgggg acgcagtct ccgtcacaa gcaccatca 
      atttatatt tcggcgccg gtttcatgt ccgaaagca 
      ccggaatca tcctcaaca acgaaatgg acgacttct 
      cctccccca acatgaaca acagcttca acgtgccgc 
      cgtccgaag ccaactaca tcgagcccg ggaagcgcc 
      cgttgtcct ccatggtgc cctcgattg tcgtggggg 
      atggcgggg ttgagctcg tgatagggg ccgcaggag 
      ggaccaaaa tcacgacct cggtggcgc agatcgccg 
      tcaagcatt tgtggtttg gggctccga tcggagcag 
      cagtgcagg agaagcgga ttcaccatc agttgttcc 
      cgatggaga ttgagttcg agaatgcgt ttaatgaaa 
      cgagagatt ttgtggaag cgttgcata aaattgggc 
      atgagtata cgatagctg agccggggt cggggtttt 
      cggcggtga cggccatct cccggaaag ggggcgatg 
      tggcgacgg cgagtgatg tgcggagga cgggcgacg 
      aagttatag tttgatggt ggagatagt taggtttat 
      ttattatgg aaatttaat ttatttgca aataaaacc 
      actgattcg ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY