Status
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Gene information
General information
TC006253 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5839607..5866124
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006253
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04099

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006253 RA
      MSSVKVAVR VRPFNNREI SRECKCIIK MGGNTTTIT 
      NPKADPKEA LKSFNFDYS YWSHDETDP EFSSQLVVY 
      KDIGEEMLQ HSFDGYNVC IFAYGQTGA GKSYTMMGK 
      QEEGQEGII PQICKDLFN RIRNNDNPD MKYSVEVSY 
      MEIYCERVR DLLNPKNKG NLRVREHPL LGPYVEDLS 
      KLAVTSYED IHDLIDEGN KARTVAATN MNETSSRSH 
      AVFTIFFTQ QRYDQTTTL CTEKVSKIS LVDLAGSER 
      ADSTGAKGT RLKEGANIN KSLTTLGKV ISALAEIAS 
      KNKKSKKAD FIPYRDSVL TWLLRENLG GNSKTAMIA 
      AISPADINY DETLSTLRY ADRAKQIVC KAVVNEDAN 
      AKLIRELKE EIQKLRELL KAEGIEVHE GAEDNIIAR 
      NNKENERSR TTSTTIAEE AVDQLQASE KLIAELNET 
      WEEKLKRTE AIRIQREAV FAEMGVAVK EDGITVGVF 
      SPKCTPHLV NLNEDPFMS ECLIYYTKD GITRVGSAE 
      ANIPQDIQL VGSHIKSEH CIFENKDGK VTLTPSAGA 
      LIYVNGREI TEPVVLKTG SRVILGKNH VFRFTHPDE 
      AREMREKNS TPLETVDWN YAQIELLEK QGVDLKAEM 
      EKRLLTLEE QYRKEKETA DQLFEEQRK NYEARIDAL 
      QKQVEEQSM TMSMYSSYT PEDFNNEDD IFECNWTEK 
      EYELAANAW RKWKHHQFT SLRDDLWGN AIFLKEANA 
      ISVELKKKV QFQFTLLTD TLYSPLPAD LRPTIDYDQ 
      DEDASVPKT IVAVEVQDT KNGATHYWS LDKLRQRLE 
      LMRLVYNEE VVELPSPEV GIETTTPLL RCLTQCPPS 
      PSRFSLTSL LPSRQRLEL MREMYHNEA EMSPTSPDY 
      NVESLTGGD PFYDRFPWF RMVGRGFVY LSNLLYPVT 
      LIHKVAIVS EKGDVRGYL RVAVQAVMD EDNSDQVGV 
      RQSARIAFE TPKAVIEHK NIHNMDDRI IEGQSAIDP 
      IKIDDLIEC DADSGRGDS SVSSDLKED EVPDHLAIG 
      KEFTFRVTV LQAVGISTE YADIFCQFN FLHRHDEAF 
      STEPVKNSG KGTPLGFYH VQNITVSTT KSFIDYIKT 
      QPIVFEVFG HYQQHPLHK DAKLEGNVR QPPRRMLPP 
      SIPISQPVR SSKFGALPS PCTAHIHAK VDVLVWFEI 
      CELAPSGEY VPVVVEHSD DLPCRGLFL LHQGIQRRI 
      RITIVHDQA AEIKWRDVR ELVVGRIRN SPESDEDED 
      NDNSVLSLG LFPGEYLEV PGDDRVMFR FEAAWDSSL 
      HNSPLLNRV TAQGEQIFM TISAYLELE NCGRPAIIT 
      KDLSMVIYG RDARTGPRS LKHLFSGNY RNAEANRLS 
      GVYELLLRR ASEAGSPGV QRRQRRVLD TSSTYVRGE 
      ENLHGWRPR GDSLIFDHQ WELEKLTRL EEVERVRHT 
      LLLRERLGL DRSTNHNKY HLDYTKSEK EVCNMVAKN 
      NNLNATNDF YEYTDREKQ LLMKCVRLI QGRGRESIT 
      GMKEKEVAS PEEMTEMST SMLSSMVSN GSIELCSPE 
      RAALPGECA PFPPVPVSP PGPRDPELV LYVPDMEEI 
      RISPVVARK GYLNVLEHK THGWKKRWV AVRRPYVFI 
      FRDEKDPVE RALINLATA QVEYSEDQL AMVRLPNTF 
      SVVSKHRGY LLQTLHEKE VHDWLYAIN PLLAGQIRS 
      KSARRNPTR QENTETTVA CK
    • >TC006253 RB
      MSSVKVAVR VRPFNNREI SRECKCIIK MGGNTTTIT 
      NPKADPKEA LKSFNFDYS YWSHDETDP EFSSQLVVY 
      KDIGEEMLQ HSFDGYNVC IFAYGQTGA GKSYTMMGK 
      QEEGQEGII PQICKDLFN RIRNNDNPD MKYSVEVSY 
      MEIYCERVR DLLNPKNKG NLRVREHPL LGPYVEDLS 
      KLAVTSYED IHDLIDEGN KARTVAATN MNETSSRSH 
      AVFTIFFTQ QRYDQTTTL CTEKVSKIS LVDLAGSER 
      ADSTGAKGT RLKEGANIN KSLTTLGKV ISALAEIAS 
      KNKKSKKAD FIPYRDSVL TWLLRENLG GNSKTAMIA 
      AISPADINY DETLSTLRY ADRAKQIVC KAVVNEDAN 
      AKLIRELKE EIQKLRELL KAEGIEVHE GAEDNIIAR 
      NNKENERSR TTSTTIAEE AVDQLQASE KLIAELNET 
      WEEKLKRTE AIRIQREAV FAEMGVAVK EDGITVGVF 
      SPKCTPHLV NLNEDPFMS ECLIYYTKD GITRVGSAE 
      ANIPQDIQL VGSHIKSEH CIFENKDGK VTLTPSAGA 
      LIYVNGREI TEPVVLKTG SRVILGKNH VFRFTHPDE 
      AREMREKNS TPLETVDWN YAQIELLEK QGVDLKAEM 
      EKRLLTLEE QYRKEKETA DQLFEEQRK NYEARIDAL 
      QKQVEEQSM TMSMYSSYT PEDFNNEDD IFVNPLFDA 
      ECNWTEKEY ELAANAWRK WKHHQFTSL RDDLWGNAI 
      FLKEANAIS VELKKKVQF QFTLLTDTL YSPLPADLR 
      PTIDYDQDE DASVPKTIV AVEVQDTKN GATHYWSLD 
      KLRQRLELM RLVYNEEVV ELPSPEVGI ETTTPLLRC 
      LTQCPPSPS RFSLTSLLP SRQRLELMR EMYHNEAEM 
      SPTSPDYNV ESLTGGDPF YDRFPWFRM VGRGFVYLS 
      NLLYPVTLI HKVAIVSEK GDVRGYLRV AVQAVMDED 
      NSDQVGVRQ SARIAFETP KAVIEHKNI HNMDDRIIE 
      GQSAIDPIK IDDLIECDA DSGRGDSSV SSDLKEDEV 
      PDHLAIGKE FTFRVTVLQ AVGISTEYA DIFCQFNFL 
      HRHDEAFST EPVKNSGKG TPLGFYHVQ NITVSTTKS 
      FIDYIKTQP IVFEVFGHY QQHPLHKDA KLEGNVRQP 
      PRRMLPPSI PISQPVRSS KFGALPSPC TAHIHAKVD 
      VLVWFEICE LAPSGEYVP VVVEHSDDL PCRGLFLLH 
      QGIQRRIRI TIVHDQAAE IKWRDVREL VVGRIRNSP 
      ESDEDEDND NSVLSLGLF PGEYLEVPG DDRVMFRFE 
      AAWDSSLHN SPLLNRVTA QGEQIFMTI SAYLELENC 
      GRPAIITKD LSMVIYGRD ARTGPRSLK HLFSGNYRN 
      AEANRLSGV YELLLRRAS EAGSPGVQR RQRRVLDTS 
      STYVRGEEN LHGWRPRGD SLIFDHQWE LEKLTRLEE 
      VERVRHTLL LRERLGLDR STNHNKYHL DYTKSEKEV 
      CNMVAKNNN LNATNDFYE YTDREKQLL MKCVRLIQG 
      RGRESITGM KEKEVASPE EMTEMSTSM LSSMVSNGS 
      IELCSPERA ALPGECAPF PPVPVSPPG PRDPELVLY 
      VPDMEEIRI SPVVARKGY LNVLEHKTH GWKKRWVAV 
      RRPYVFIFR DEKDPVERA LINLATAQV EYSEDQLAM 
      VRLPNTFSV VSKHRGYLL QTLHEKEVH DWLYAINPL 
      LAGQIRSKS ARRNPTRQE NTETTVACK
    CDS sequence
    • >TC006253 RA
      atgtcgtca gtcaaagta gcagttcgt gtccggccg 
      ttcaacaac cgagaaatc tcgcgagaa tgcaaatgt 
      ataataaaa atgggagga aacactaca acaatcaca 
      aatccaaaa gccgacccg aaagaagcc ctcaaaagc 
      ttcaatttc gactattcc tactggtca cacgatgag 
      accgacccg gaattctcc tcacaactg gtcgtctac 
      aaggacatc ggcgaggaa atgctccag cactccttc 
      gacggctac aacgtttgc atcttcgcc tacgggcag 
      accggcgcc ggcaagtcc tacaccatg atgggcaag 
      caggaggag ggccaagaa ggcatcatc ccccagatc 
      tgcaaggac ctcttcaac aggatacgc aacaacgac 
      aaccccgac atgaagtac tcggtggag gtcagctac 
      atggagatc tactgcgag cgggtgcgc gatctgctc 
      aaccccaag aacaagggg aacttgcgg gtgcgcgag 
      caccccctt ctggggccc tacgtcgag gatctgagc 
      aagctggcg gtgacgagc tacgaggac atccacgac 
      ctgatagat gagggcaac aaggccaga acggtggcc 
      gccactaac atgaacgag accagttcg aggtcgcat 
      gcggtgttt acgattttt ttcacgcaa cagagatac 
      gatcaaacg acgacattg tgcacggag aaggtctcc 
      aagatctcg ctggtggac ctggcgggc tccgagagg 
      gccgactcc acgggggcc aagggcacg aggctcaag 
      gagggcgcc aacatcaac aagagtctg acgacgttg 
      ggcaaggtc atctcggcg ctcgctgaa atcgcgtct 
      aagaacaag aaatcaaaa aaagccgac tttatccct 
      taccgagac tcggttttg acatggttg ctgcgcgag 
      aacctcgga ggcaacagc aagactgca atgatagcc 
      gccatatct ccagccgat atcaactac gacgagact 
      ttgtccact ctcaggtac gctgatcga gccaagcag 
      atcgtgtgc aaggccgtg gttaacgag gacgccaac 
      gcgaaactc atccgagag ctcaaagag gagatccag 
      aaactccgc gagctgctc aaggccgaa ggcatcgaa 
      gtgcacgag ggagctgaa gataacatc atcgctcgc 
      aacaataaa gaaaacgag agatcgaga acgacttca 
      acgacaata gccgaggaa gccgtcgat caattgcaa 
      gccagcgag aaactcatc gccgaactg aacgaaaca 
      tgggaggag aaattgaag cgcaccgaa gctataaga 
      atccaacgg gaggccgtt ttcgccgaa atgggggtg 
      gcggtgaag gaggacggg atcacggtt ggggtgttc 
      tcccccaaa tgtaccccc catttggtg aatttgaac 
      gaggatccg ttcatgtcc gaatgcctg atttactac 
      accaaggac ggtattacg agggtcggg tcagccgag 
      gcaaacatt ccgcaagat atacagctc gtggggtcg 
      cacattaaa tcagagcat tgcattttt gagaataag 
      gacgggaaa gtgacactg actccatca gctggggct 
      ttaatttat gtcaatgga cgggagatc accgagcca 
      gtcgtcctc aaaaccggc tcccgagtc attttgggc 
      aaaaaccac gtgttccgc ttcacgcat ccagacgaa 
      gcgcgtgag atgcgtgaa aaaaattcg acaccttta 
      gaaaccgtt gactggaac tatgcacaa attgaactt 
      cttgaaaaa caaggcgtt gacttaaag gccgagatg 
      gaaaaacgt ctcctaacc ctcgaagaa caataccgt 
      aaggaaaaa gaaaccgca gatcaactg tttgaagag 
      cagcgaaaa aattacgag gctcgaatt gacgcgctt 
      caaaagcaa gtggaagaa cagagcatg acaatgtcg 
      atgtacagt tcctatacg cccgaggat ttcaataat 
      gaagatgat attttcgag tgcaattgg accgagaag 
      gagtacgag ctggccgcc aatgcctgg aggaagtgg 
      aaacatcac cagtttacc tccttgagg gacgatctc 
      tggggcaat gccattttc ctcaaggag gcgaacgcc 
      atttcggtc gaactcaag aagaaagta caattccaa 
      ttcacactt ttgaccgac accctctac tcgcccctt 
      ccggccgat ttgcgtccc accatcgac tatgatcaa 
      gacgaggac gcgtcagtg ccgaaaaca attgtcgct 
      gttgaagtt caagatacg aaaaacggg gcgactcat 
      tactggtcg ttggataaa cttcgtcaa agactggaa 
      ttgatgcgt ctggtttat aacgaggaa gttgtggaa 
      ttgccgagt cctgaagtt ggtattgaa acaacaacg 
      cctttgctg agatgtctc acgcaatgt ccaccatca 
      ccgtcacgc ttctctctt accagtctg ttgccgtcg 
      aggcaaagg ctggaactc atgcgcgaa atgtaccac 
      aacgaggcc gaaatgtca ccgacttcg cccgattat 
      aatgtggag tcgctcact gggggcgac cccttctat 
      gaccgtttt ccctggttt agaatggtg gggcgaggg 
      tttgtctac ttgagcaat cttttatac cccgtgacg 
      ctgattcat aaagtggcg attgttagt gaaaaagga 
      gatgttcga ggatatttg agagtagcg gttcaggcc 
      gttatggat gaggataat tcggaccaa gttggtgtg 
      aggcaaagt gcaaggata gcttttgaa acacccaaa 
      gccgttatt gaacataaa aatattcac aatatggac 
      gatcgcatt atcgaaggt cagagcgct attgatccg 
      attaaaatc gatgatctt atagagtgt gatgcagat 
      tcaggacgt ggcgatagt tccgtttcg tcagattta 
      aaagaggat gaagttccc gatcattta gcgatcgga 
      aaagagttt actttcaga gttactgtc ttacaagca 
      gtgggaatt tccacggaa tatgccgat atcttctgc 
      caattcaat ttcttgcat agacatgac gaggctttt 
      tcgacagaa ccagtcaaa aattcgggc aaaggcact 
      ccacttggc ttctaccac gtacaaaat atcacagtt 
      tcgaccacg aaatcattc atcgactat atcaaaacc 
      cagccaatt gtcttcgaa gtttttggc cactaccaa 
      cagcacccc cttcacaaa gacgccaaa ttggagggc 
      aacgtccgc cagccccct cgccgcatg ctcccgccc 
      tccatcccc atttcgcag ccagtcaga tcgagcaaa 
      ttcggcgct ttgccctcc ccctgcacc gctcacatt 
      cacgccaaa gtggacgtt ttagtttgg tttgaaatc 
      tgcgaattg gccccgagt ggcgaatac gtccctgtt 
      gtggtggag cacagtgat gatttgcct tgtcgtgga 
      ttgtttttg ttgcatcag gggattcaa agacggatt 
      aggattaca attgttcat gatcaagcc gctgagatt 
      aaatggaga gatgtgaga gagttggta gtgggaagg 
      attaggaat agtccggag tcggacgag gatgaggat 
      aatgataat tcggtgctg tcgttggga ttattccca 
      ggggagtat ctggaggtg cctggggat gatagggtg 
      atgtttagg tttgaggcg gcctgggat agctcgctg 
      cataattca ccattgctg aatagggtc acggcgcaa 
      ggggagcag attttcatg accatctcg gcttatttg 
      gagttggaa aattgcggc cgccctgcc attataaca 
      aaagacctg agtatggta atctacgga agagacgct 
      cgaactggc cctcgatcc ctcaaacac ctcttctct 
      ggaaactat cgcaacgcc gaagcaaac cgcctatca 
      ggagtctac gaattactt ttgcgacgt gctagtgaa 
      gcaggtagt ccaggtgtg caaagacgc caacgtcgc 
      gtcctggac accagctcc acctacgtc cgcggcgag 
      gaaaacctc cacggttgg cgtcctcgt ggcgattcg 
      ttaatcttc gaccaccaa tgggagctc gagaaattg 
      acccgactt gaagaagtc gaacgtgtg cgccacact 
      ctcctcctc cgcgagcgt ctaggtctc gaccgttca 
      accaatcac aacaagtat cacctcgac tacacaaaa 
      agcgagaag gaagtgtgc aacatggtc gctaaaaac 
      aacaacctc aacgcaacc aacgatttt tacgaatac 
      accgatcgc gagaaacaa ctcttaatg aaatgcgtc 
      cgcttgatc cagggacga ggccgcgaa agcatcacc 
      ggaatgaag gaaaaagag gtggcaagt ccggaagaa 
      atgaccgaa atgagcacg agcatgcta tcaagcatg 
      gtgtcgaac gggtcgatc gagctctgc tcaccggaa 
      cgggcggcg cttccgggc gaatgtgcc ccgttcccg 
      ccggttccg gtctctccc ccaggcccc agggacccg 
      gagttggtg ctctacgtc ccggacatg gaggaaatt 
      aggatttcg cccgtcgtc gcacgcaag gggtatttg 
      aacgttttg gagcataaa acgcacggg tggaagaag 
      cgatgggtg gccgtccga cgaccctac gtgtttatt 
      ttccgggat gaaaaagat ccagttgaa cgggcgctt 
      attaaccta gctacggcc caagtggag tattccgag 
      gatcagttg gcgatggtt cgactcccg aatactttc 
      agcgtggtg agcaaacat cgcggttat ctccttcaa 
      acgcttcac gagaaggag gtgcacgat tggttgtat 
      gccattaat ccgttatta gctgggcaa attcgctcg 
      aaatcggcc agacgtaat ccaacacgt caggaaaat 
      acggaaact acggtggca tgtaaatga
    • >TC006253 RB
      atgtcgtca gtcaaagta gcagttcgt gtccggccg 
      ttcaacaac cgagaaatc tcgcgagaa tgcaaatgt 
      ataataaaa atgggagga aacactaca acaatcaca 
      aatccaaaa gccgacccg aaagaagcc ctcaaaagc 
      ttcaatttc gactattcc tactggtca cacgatgag 
      accgacccg gaattctcc tcacaactg gtcgtctac 
      aaggacatc ggcgaggaa atgctccag cactccttc 
      gacggctac aacgtttgc atcttcgcc tacgggcag 
      accggcgcc ggcaagtcc tacaccatg atgggcaag 
      caggaggag ggccaagaa ggcatcatc ccccagatc 
      tgcaaggac ctcttcaac aggatacgc aacaacgac 
      aaccccgac atgaagtac tcggtggag gtcagctac 
      atggagatc tactgcgag cgggtgcgc gatctgctc 
      aaccccaag aacaagggg aacttgcgg gtgcgcgag 
      caccccctt ctggggccc tacgtcgag gatctgagc 
      aagctggcg gtgacgagc tacgaggac atccacgac 
      ctgatagat gagggcaac aaggccaga acggtggcc 
      gccactaac atgaacgag accagttcg aggtcgcat 
      gcggtgttt acgattttt ttcacgcaa cagagatac 
      gatcaaacg acgacattg tgcacggag aaggtctcc 
      aagatctcg ctggtggac ctggcgggc tccgagagg 
      gccgactcc acgggggcc aagggcacg aggctcaag 
      gagggcgcc aacatcaac aagagtctg acgacgttg 
      ggcaaggtc atctcggcg ctcgctgaa atcgcgtct 
      aagaacaag aaatcaaaa aaagccgac tttatccct 
      taccgagac tcggttttg acatggttg ctgcgcgag 
      aacctcgga ggcaacagc aagactgca atgatagcc 
      gccatatct ccagccgat atcaactac gacgagact 
      ttgtccact ctcaggtac gctgatcga gccaagcag 
      atcgtgtgc aaggccgtg gttaacgag gacgccaac 
      gcgaaactc atccgagag ctcaaagag gagatccag 
      aaactccgc gagctgctc aaggccgaa ggcatcgaa 
      gtgcacgag ggagctgaa gataacatc atcgctcgc 
      aacaataaa gaaaacgag agatcgaga acgacttca 
      acgacaata gccgaggaa gccgtcgat caattgcaa 
      gccagcgag aaactcatc gccgaactg aacgaaaca 
      tgggaggag aaattgaag cgcaccgaa gctataaga 
      atccaacgg gaggccgtt ttcgccgaa atgggggtg 
      gcggtgaag gaggacggg atcacggtt ggggtgttc 
      tcccccaaa tgtaccccc catttggtg aatttgaac 
      gaggatccg ttcatgtcc gaatgcctg atttactac 
      accaaggac ggtattacg agggtcggg tcagccgag 
      gcaaacatt ccgcaagat atacagctc gtggggtcg 
      cacattaaa tcagagcat tgcattttt gagaataag 
      gacgggaaa gtgacactg actccatca gctggggct 
      ttaatttat gtcaatgga cgggagatc accgagcca 
      gtcgtcctc aaaaccggc tcccgagtc attttgggc 
      aaaaaccac gtgttccgc ttcacgcat ccagacgaa 
      gcgcgtgag atgcgtgaa aaaaattcg acaccttta 
      gaaaccgtt gactggaac tatgcacaa attgaactt 
      cttgaaaaa caaggcgtt gacttaaag gccgagatg 
      gaaaaacgt ctcctaacc ctcgaagaa caataccgt 
      aaggaaaaa gaaaccgca gatcaactg tttgaagag 
      cagcgaaaa aattacgag gctcgaatt gacgcgctt 
      caaaagcaa gtggaagaa cagagcatg acaatgtcg 
      atgtacagt tcctatacg cccgaggat ttcaataat 
      gaagatgat attttcgtg aaccctctg tttgacgca 
      gagtgcaat tggaccgag aaggagtac gagctggcc 
      gccaatgcc tggaggaag tggaaacat caccagttt 
      acctccttg agggacgat ctctggggc aatgccatt 
      ttcctcaag gaggcgaac gccatttcg gtcgaactc 
      aagaagaaa gtacaattc caattcaca cttttgacc 
      gacaccctc tactcgccc cttccggcc gatttgcgt 
      cccaccatc gactatgat caagacgag gacgcgtca 
      gtgccgaaa acaattgtc gctgttgaa gttcaagat 
      acgaaaaac ggggcgact cattactgg tcgttggat 
      aaacttcgt caaagactg gaattgatg cgtctggtt 
      tataacgag gaagttgtg gaattgccg agtcctgaa 
      gttggtatt gaaacaaca acgcctttg ctgagatgt 
      ctcacgcaa tgtccacca tcaccgtca cgcttctct 
      cttaccagt ctgttgccg tcgaggcaa aggctggaa 
      ctcatgcgc gaaatgtac cacaacgag gccgaaatg 
      tcaccgact tcgcccgat tataatgtg gagtcgctc 
      actgggggc gaccccttc tatgaccgt tttccctgg 
      tttagaatg gtggggcga gggtttgtc tacttgagc 
      aatctttta taccccgtg acgctgatt cataaagtg 
      gcgattgtt agtgaaaaa ggagatgtt cgaggatat 
      ttgagagta gcggttcag gccgttatg gatgaggat 
      aattcggac caagttggt gtgaggcaa agtgcaagg 
      atagctttt gaaacaccc aaagccgtt attgaacat 
      aaaaatatt cacaatatg gacgatcgc attatcgaa 
      ggtcagagc gctattgat ccgattaaa atcgatgat 
      cttatagag tgtgatgca gattcagga cgtggcgat 
      agttccgtt tcgtcagat ttaaaagag gatgaagtt 
      cccgatcat ttagcgatc ggaaaagag tttactttc 
      agagttact gtcttacaa gcagtggga atttccacg 
      gaatatgcc gatatcttc tgccaattc aatttcttg 
      catagacat gacgaggct ttttcgaca gaaccagtc 
      aaaaattcg ggcaaaggc actccactt ggcttctac 
      cacgtacaa aatatcaca gtttcgacc acgaaatca 
      ttcatcgac tatatcaaa acccagcca attgtcttc 
      gaagttttt ggccactac caacagcac ccccttcac 
      aaagacgcc aaattggag ggcaacgtc cgccagccc 
      cctcgccgc atgctcccg ccctccatc cccatttcg 
      cagccagtc agatcgagc aaattcggc gctttgccc 
      tccccctgc accgctcac attcacgcc aaagtggac 
      gttttagtt tggtttgaa atctgcgaa ttggccccg 
      agtggcgaa tacgtccct gttgtggtg gagcacagt 
      gatgatttg ccttgtcgt ggattgttt ttgttgcat 
      caggggatt caaagacgg attaggatt acaattgtt 
      catgatcaa gccgctgag attaaatgg agagatgtg 
      agagagttg gtagtggga aggattagg aatagtccg 
      gagtcggac gaggatgag gataatgat aattcggtg 
      ctgtcgttg ggattattc ccaggggag tatctggag 
      gtgcctggg gatgatagg gtgatgttt aggtttgag 
      gcggcctgg gatagctcg ctgcataat tcaccattg 
      ctgaatagg gtcacggcg caaggggag cagattttc 
      atgaccatc tcggcttat ttggagttg gaaaattgc 
      ggccgccct gccattata acaaaagac ctgagtatg 
      gtaatctac ggaagagac gctcgaact ggccctcga 
      tccctcaaa cacctcttc tctggaaac tatcgcaac 
      gccgaagca aaccgccta tcaggagtc tacgaatta 
      cttttgcga cgtgctagt gaagcaggt agtccaggt 
      gtgcaaaga cgccaacgt cgcgtcctg gacaccagc 
      tccacctac gtccgcggc gaggaaaac ctccacggt 
      tggcgtcct cgtggcgat tcgttaatc ttcgaccac 
      caatgggag ctcgagaaa ttgacccga cttgaagaa 
      gtcgaacgt gtgcgccac actctcctc ctccgcgag 
      cgtctaggt ctcgaccgt tcaaccaat cacaacaag 
      tatcacctc gactacaca aaaagcgag aaggaagtg 
      tgcaacatg gtcgctaaa aacaacaac ctcaacgca 
      accaacgat ttttacgaa tacaccgat cgcgagaaa 
      caactctta atgaaatgc gtccgcttg atccaggga 
      cgaggccgc gaaagcatc accggaatg aaggaaaaa 
      gaggtggca agtccggaa gaaatgacc gaaatgagc 
      acgagcatg ctatcaagc atggtgtcg aacgggtcg 
      atcgagctc tgctcaccg gaacgggcg gcgcttccg 
      ggcgaatgt gccccgttc ccgccggtt ccggtctct 
      cccccaggc cccagggac ccggagttg gtgctctac 
      gtcccggac atggaggaa attaggatt tcgcccgtc 
      gtcgcacgc aaggggtat ttgaacgtt ttggagcat 
      aaaacgcac gggtggaag aagcgatgg gtggccgtc 
      cgacgaccc tacgtgttt attttccgg gatgaaaaa 
      gatccagtt gaacgggcg cttattaac ctagctacg 
      gcccaagtg gagtattcc gaggatcag ttggcgatg 
      gttcgactc ccgaatact ttcagcgtg gtgagcaaa 
      catcgcggt tatctcctt caaacgctt cacgagaag 
      gaggtgcac gattggttg tatgccatt aatccgtta 
      ttagctggg caaattcgc tcgaaatcg gccagacgt 
      aatccaaca cgtcaggaa aatacggaa actacggtg 
      gcatgtaaa tga
    mRNA sequence
    • >TC006253 RA
      ttattaaca aatgtcatg tagtgtttg tttctgcaa 
      gaaaaatcg taaataaag acaataagt tttagggaa 
      acataaata tcaaacatg tcgtcagtc aaagtagca 
      gttcgtgtc cggccgttc aacaaccga gaaatctcg 
      cgagaatgc aaatgtata ataaaaatg ggaggaaac 
      actacaaca atcacaaat ccaaaagcc gacccgaaa 
      gaagccctc aaaagcttc aatttcgac tattcctac 
      tggtcacac gatgagacc gacccggaa ttctcctca 
      caactggtc gtctacaag gacatcggc gaggaaatg 
      ctccagcac tccttcgac ggctacaac gtttgcatc 
      ttcgcctac gggcagacc ggcgccggc aagtcctac 
      accatgatg ggcaagcag gaggagggc caagaaggc 
      atcatcccc cagatctgc aaggacctc ttcaacagg 
      atacgcaac aacgacaac cccgacatg aagtactcg 
      gtggaggtc agctacatg gagatctac tgcgagcgg 
      gtgcgcgat ctgctcaac cccaagaac aaggggaac 
      ttgcgggtg cgcgagcac ccccttctg gggccctac 
      gtcgaggat ctgagcaag ctggcggtg acgagctac 
      gaggacatc cacgacctg atagatgag ggcaacaag 
      gccagaacg gtggccgcc actaacatg aacgagacc 
      agttcgagg tcgcatgcg gtgtttacg atttttttc 
      acgcaacag agatacgat caaacgacg acattgtgc 
      acggagaag gtctccaag atctcgctg gtggacctg 
      gcgggctcc gagagggcc gactccacg ggggccaag 
      ggcacgagg ctcaaggag ggcgccaac atcaacaag 
      agtctgacg acgttgggc aaggtcatc tcggcgctc 
      gctgaaatc gcgtctaag aacaagaaa tcaaaaaaa 
      gccgacttt atcccttac cgagactcg gttttgaca 
      tggttgctg cgcgagaac ctcggaggc aacagcaag 
      actgcaatg atagccgcc atatctcca gccgatatc 
      aactacgac gagactttg tccactctc aggtacgct 
      gatcgagcc aagcagatc gtgtgcaag gccgtggtt 
      aacgaggac gccaacgcg aaactcatc cgagagctc 
      aaagaggag atccagaaa ctccgcgag ctgctcaag 
      gccgaaggc atcgaagtg cacgaggga gctgaagat 
      aacatcatc gctcgcaac aataaagaa aacgagaga 
      tcgagaacg acttcaacg acaatagcc gaggaagcc 
      gtcgatcaa ttgcaagcc agcgagaaa ctcatcgcc 
      gaactgaac gaaacatgg gaggagaaa ttgaagcgc 
      accgaagct ataagaatc caacgggag gccgttttc 
      gccgaaatg ggggtggcg gtgaaggag gacgggatc 
      acggttggg gtgttctcc cccaaatgt accccccat 
      ttggtgaat ttgaacgag gatccgttc atgtccgaa 
      tgcctgatt tactacacc aaggacggt attacgagg 
      gtcgggtca gccgaggca aacattccg caagatata 
      cagctcgtg gggtcgcac attaaatca gagcattgc 
      atttttgag aataaggac gggaaagtg acactgact 
      ccatcagct ggggcttta atttatgtc aatggacgg 
      gagatcacc gagccagtc gtcctcaaa accggctcc 
      cgagtcatt ttgggcaaa aaccacgtg ttccgcttc 
      acgcatcca gacgaagcg cgtgagatg cgtgaaaaa 
      aattcgaca cctttagaa accgttgac tggaactat 
      gcacaaatt gaacttctt gaaaaacaa ggcgttgac 
      ttaaaggcc gagatggaa aaacgtctc ctaaccctc 
      gaagaacaa taccgtaag gaaaaagaa accgcagat 
      caactgttt gaagagcag cgaaaaaat tacgaggct 
      cgaattgac gcgcttcaa aagcaagtg gaagaacag 
      agcatgaca atgtcgatg tacagttcc tatacgccc 
      gaggatttc aataatgaa gatgatatt ttcgagtgc 
      aattggacc gagaaggag tacgagctg gccgccaat 
      gcctggagg aagtggaaa catcaccag tttacctcc 
      ttgagggac gatctctgg ggcaatgcc attttcctc 
      aaggaggcg aacgccatt tcggtcgaa ctcaagaag 
      aaagtacaa ttccaattc acacttttg accgacacc 
      ctctactcg ccccttccg gccgatttg cgtcccacc 
      atcgactat gatcaagac gaggacgcg tcagtgccg 
      aaaacaatt gtcgctgtt gaagttcaa gatacgaaa 
      aacggggcg actcattac tggtcgttg gataaactt 
      cgtcaaaga ctggaattg atgcgtctg gtttataac 
      gaggaagtt gtggaattg ccgagtcct gaagttggt 
      attgaaaca acaacgcct ttgctgaga tgtctcacg 
      caatgtcca ccatcaccg tcacgcttc tctcttacc 
      agtctgttg ccgtcgagg caaaggctg gaactcatg 
      cgcgaaatg taccacaac gaggccgaa atgtcaccg 
      acttcgccc gattataat gtggagtcg ctcactggg 
      ggcgacccc ttctatgac cgttttccc tggtttaga 
      atggtgggg cgagggttt gtctacttg agcaatctt 
      ttatacccc gtgacgctg attcataaa gtggcgatt 
      gttagtgaa aaaggagat gttcgagga tatttgaga 
      gtagcggtt caggccgtt atggatgag gataattcg 
      gaccaagtt ggtgtgagg caaagtgca aggatagct 
      tttgaaaca cccaaagcc gttattgaa cataaaaat 
      attcacaat atggacgat cgcattatc gaaggtcag 
      agcgctatt gatccgatt aaaatcgat gatcttata 
      gagtgtgat gcagattca ggacgtggc gatagttcc 
      gtttcgtca gatttaaaa gaggatgaa gttcccgat 
      catttagcg atcggaaaa gagtttact ttcagagtt 
      actgtctta caagcagtg ggaatttcc acggaatat 
      gccgatatc ttctgccaa ttcaatttc ttgcataga 
      catgacgag gctttttcg acagaacca gtcaaaaat 
      tcgggcaaa ggcactcca cttggcttc taccacgta 
      caaaatatc acagtttcg accacgaaa tcattcatc 
      gactatatc aaaacccag ccaattgtc ttcgaagtt 
      tttggccac taccaacag caccccctt cacaaagac 
      gccaaattg gagggcaac gtccgccag ccccctcgc 
      cgcatgctc ccgccctcc atccccatt tcgcagcca 
      gtcagatcg agcaaattc ggcgctttg ccctccccc 
      tgcaccgct cacattcac gccaaagtg gacgtttta 
      gtttggttt gaaatctgc gaattggcc ccgagtggc 
      gaatacgtc cctgttgtg gtggagcac agtgatgat 
      ttgccttgt cgtggattg tttttgttg catcagggg 
      attcaaaga cggattagg attacaatt gttcatgat 
      caagccgct gagattaaa tggagagat gtgagagag 
      ttggtagtg ggaaggatt aggaatagt ccggagtcg 
      gacgaggat gaggataat gataattcg gtgctgtcg 
      ttgggatta ttcccaggg gagtatctg gaggtgcct 
      ggggatgat agggtgatg tttaggttt gaggcggcc 
      tgggatagc tcgctgcat aattcacca ttgctgaat 
      agggtcacg gcgcaaggg gagcagatt ttcatgacc 
      atctcggct tatttggag ttggaaaat tgcggccgc 
      cctgccatt ataacaaaa gacctgagt atggtaatc 
      tacggaaga gacgctcga actggccct cgatccctc 
      aaacacctc ttctctgga aactatcgc aacgccgaa 
      gcaaaccgc ctatcagga gtctacgaa ttacttttg 
      cgacgtgct agtgaagca ggtagtcca ggtgtgcaa 
      agacgccaa cgtcgcgtc ctggacacc agctccacc 
      tacgtccgc ggcgaggaa aacctccac ggttggcgt 
      cctcgtggc gattcgtta atcttcgac caccaatgg 
      gagctcgag aaattgacc cgacttgaa gaagtcgaa 
      cgtgtgcgc cacactctc ctcctccgc gagcgtcta 
      ggtctcgac cgttcaacc aatcacaac aagtatcac 
      ctcgactac acaaaaagc gagaaggaa gtgtgcaac 
      atggtcgct aaaaacaac aacctcaac gcaaccaac 
      gatttttac gaatacacc gatcgcgag aaacaactc 
      ttaatgaaa tgcgtccgc ttgatccag ggacgaggc 
      cgcgaaagc atcaccgga atgaaggaa aaagaggtg 
      gcaagtccg gaagaaatg accgaaatg agcacgagc 
      atgctatca agcatggtg tcgaacggg tcgatcgag 
      ctctgctca ccggaacgg gcggcgctt ccgggcgaa 
      tgtgccccg ttcccgccg gttccggtc tctccccca 
      ggccccagg gacccggag ttggtgctc tacgtcccg 
      gacatggag gaaattagg atttcgccc gtcgtcgca 
      cgcaagggg tatttgaac gttttggag cataaaacg 
      cacgggtgg aagaagcga tgggtggcc gtccgacga 
      ccctacgtg tttattttc cgggatgaa aaagatcca 
      gttgaacgg gcgcttatt aacctagct acggcccaa 
      gtggagtat tccgaggat cagttggcg atggttcga 
      ctcccgaat actttcagc gtggtgagc aaacatcgc 
      ggttatctc cttcaaacg cttcacgag aaggaggtg 
      cacgattgg ttgtatgcc attaatccg ttattagct 
      gggcaaatt cgctcgaaa tcggccaga cgtaatcca 
      acacgtcag gaaaatacg gaaactacg gtggcatgt 
      aaatgagat ttaatcgtt atggaatcc gttttggtt 
      tattaggtt tttttgagt tgattgagg ttgtgatag 
      ggttccata aacggggga tttggttct tctagccca 
      ggtgttgcg ggattgttt ggcgaaatg attcgcgac 
      acctgggct gtttctagt cgagcattt ctttacaaa 
      tctagtcga ggaattgtg tgagaattt ttatgatcc 
      ggttgtgtg caataagag agtttctca gaccgtttc 
      gaatgctta tttcagttt gattgatct cagtgttaa 
      aatagtatt aaagcttct tatagtgta atgaatatt 
      taatacatg taccaatct aattaaaca tcatattct 
      accgtttta atgttaagg cctcgggtt tttctttga 
      caaagtgaa tgtaaagaa agcagaaat ttcgacact 
      ttttcgttt gattttcgg gcacagtaa atgtatgat 
      gtacagggt gtttattgt tcaatcgat gaagagctc 
      actttttga ttatttttg gaaacttcc acttcaacc 
      aaccctgtt gttattttt gtacttgaa ctagattta 
      cacgaattg tttttattg tatatacct acctaccaa 
      agttagcac aatagatct cacgctgaa accgctatt 
      gtttcaaaa ctggcattt gtaagccga agcttacat 
      ccatgacca tgtctttga ctgattttt attcgattt 
      ggttgttgt aggtgcttg ttaaactca tcataattt 
      tatttttat agttagttc ccctataga cacagtgct 
      acgcttatc taagctata aatgtaaaa aagaagtac 
      tatttaatg aaattagta ggtacaatg taggaaaat 
      ttactttcc ttttcttta ttttatttc ggatgctgc 
      ctttgcagg ctcattatt gattaattg aatgattat 
      tattattgt ataattgtg taagcatta tatgaaata 
      aataaagaa aaccaaaat at
    • >TC006253 RB
      ttattaaca aatgtcatg tagtgtttg tttctgcaa 
      gaaaaatcg taaataaag acaataagt tttagggaa 
      acataaata tcaaacatg tcgtcagtc aaagtagca 
      gttcgtgtc cggccgttc aacaaccga gaaatctcg 
      cgagaatgc aaatgtata ataaaaatg ggaggaaac 
      actacaaca atcacaaat ccaaaagcc gacccgaaa 
      gaagccctc aaaagcttc aatttcgac tattcctac 
      tggtcacac gatgagacc gacccggaa ttctcctca 
      caactggtc gtctacaag gacatcggc gaggaaatg 
      ctccagcac tccttcgac ggctacaac gtttgcatc 
      ttcgcctac gggcagacc ggcgccggc aagtcctac 
      accatgatg ggcaagcag gaggagggc caagaaggc 
      atcatcccc cagatctgc aaggacctc ttcaacagg 
      atacgcaac aacgacaac cccgacatg aagtactcg 
      gtggaggtc agctacatg gagatctac tgcgagcgg 
      gtgcgcgat ctgctcaac cccaagaac aaggggaac 
      ttgcgggtg cgcgagcac ccccttctg gggccctac 
      gtcgaggat ctgagcaag ctggcggtg acgagctac 
      gaggacatc cacgacctg atagatgag ggcaacaag 
      gccagaacg gtggccgcc actaacatg aacgagacc 
      agttcgagg tcgcatgcg gtgtttacg atttttttc 
      acgcaacag agatacgat caaacgacg acattgtgc 
      acggagaag gtctccaag atctcgctg gtggacctg 
      gcgggctcc gagagggcc gactccacg ggggccaag 
      ggcacgagg ctcaaggag ggcgccaac atcaacaag 
      agtctgacg acgttgggc aaggtcatc tcggcgctc 
      gctgaaatc gcgtctaag aacaagaaa tcaaaaaaa 
      gccgacttt atcccttac cgagactcg gttttgaca 
      tggttgctg cgcgagaac ctcggaggc aacagcaag 
      actgcaatg atagccgcc atatctcca gccgatatc 
      aactacgac gagactttg tccactctc aggtacgct 
      gatcgagcc aagcagatc gtgtgcaag gccgtggtt 
      aacgaggac gccaacgcg aaactcatc cgagagctc 
      aaagaggag atccagaaa ctccgcgag ctgctcaag 
      gccgaaggc atcgaagtg cacgaggga gctgaagat 
      aacatcatc gctcgcaac aataaagaa aacgagaga 
      tcgagaacg acttcaacg acaatagcc gaggaagcc 
      gtcgatcaa ttgcaagcc agcgagaaa ctcatcgcc 
      gaactgaac gaaacatgg gaggagaaa ttgaagcgc 
      accgaagct ataagaatc caacgggag gccgttttc 
      gccgaaatg ggggtggcg gtgaaggag gacgggatc 
      acggttggg gtgttctcc cccaaatgt accccccat 
      ttggtgaat ttgaacgag gatccgttc atgtccgaa 
      tgcctgatt tactacacc aaggacggt attacgagg 
      gtcgggtca gccgaggca aacattccg caagatata 
      cagctcgtg gggtcgcac attaaatca gagcattgc 
      atttttgag aataaggac gggaaagtg acactgact 
      ccatcagct ggggcttta atttatgtc aatggacgg 
      gagatcacc gagccagtc gtcctcaaa accggctcc 
      cgagtcatt ttgggcaaa aaccacgtg ttccgcttc 
      acgcatcca gacgaagcg cgtgagatg cgtgaaaaa 
      aattcgaca cctttagaa accgttgac tggaactat 
      gcacaaatt gaacttctt gaaaaacaa ggcgttgac 
      ttaaaggcc gagatggaa aaacgtctc ctaaccctc 
      gaagaacaa taccgtaag gaaaaagaa accgcagat 
      caactgttt gaagagcag cgaaaaaat tacgaggct 
      cgaattgac gcgcttcaa aagcaagtg gaagaacag 
      agcatgaca atgtcgatg tacagttcc tatacgccc 
      gaggatttc aataatgaa gatgatatt ttcgtgaac 
      cctctgttt gacgcagag tgcaattgg accgagaag 
      gagtacgag ctggccgcc aatgcctgg aggaagtgg 
      aaacatcac cagtttacc tccttgagg gacgatctc 
      tggggcaat gccattttc ctcaaggag gcgaacgcc 
      atttcggtc gaactcaag aagaaagta caattccaa 
      ttcacactt ttgaccgac accctctac tcgcccctt 
      ccggccgat ttgcgtccc accatcgac tatgatcaa 
      gacgaggac gcgtcagtg ccgaaaaca attgtcgct 
      gttgaagtt caagatacg aaaaacggg gcgactcat 
      tactggtcg ttggataaa cttcgtcaa agactggaa 
      ttgatgcgt ctggtttat aacgaggaa gttgtggaa 
      ttgccgagt cctgaagtt ggtattgaa acaacaacg 
      cctttgctg agatgtctc acgcaatgt ccaccatca 
      ccgtcacgc ttctctctt accagtctg ttgccgtcg 
      aggcaaagg ctggaactc atgcgcgaa atgtaccac 
      aacgaggcc gaaatgtca ccgacttcg cccgattat 
      aatgtggag tcgctcact gggggcgac cccttctat 
      gaccgtttt ccctggttt agaatggtg gggcgaggg 
      tttgtctac ttgagcaat cttttatac cccgtgacg 
      ctgattcat aaagtggcg attgttagt gaaaaagga 
      gatgttcga ggatatttg agagtagcg gttcaggcc 
      gttatggat gaggataat tcggaccaa gttggtgtg 
      aggcaaagt gcaaggata gcttttgaa acacccaaa 
      gccgttatt gaacataaa aatattcac aatatggac 
      gatcgcatt atcgaaggt cagagcgct attgatccg 
      attaaaatc gatgatctt atagagtgt gatgcagat 
      tcaggacgt ggcgatagt tccgtttcg tcagattta 
      aaagaggat gaagttccc gatcattta gcgatcgga 
      aaagagttt actttcaga gttactgtc ttacaagca 
      gtgggaatt tccacggaa tatgccgat atcttctgc 
      caattcaat ttcttgcat agacatgac gaggctttt 
      tcgacagaa ccagtcaaa aattcgggc aaaggcact 
      ccacttggc ttctaccac gtacaaaat atcacagtt 
      tcgaccacg aaatcattc atcgactat atcaaaacc 
      cagccaatt gtcttcgaa gtttttggc cactaccaa 
      cagcacccc cttcacaaa gacgccaaa ttggagggc 
      aacgtccgc cagccccct cgccgcatg ctcccgccc 
      tccatcccc atttcgcag ccagtcaga tcgagcaaa 
      ttcggcgct ttgccctcc ccctgcacc gctcacatt 
      cacgccaaa gtggacgtt ttagtttgg tttgaaatc 
      tgcgaattg gccccgagt ggcgaatac gtccctgtt 
      gtggtggag cacagtgat gatttgcct tgtcgtgga 
      ttgtttttg ttgcatcag gggattcaa agacggatt 
      aggattaca attgttcat gatcaagcc gctgagatt 
      aaatggaga gatgtgaga gagttggta gtgggaagg 
      attaggaat agtccggag tcggacgag gatgaggat 
      aatgataat tcggtgctg tcgttggga ttattccca 
      ggggagtat ctggaggtg cctggggat gatagggtg 
      atgtttagg tttgaggcg gcctgggat agctcgctg 
      cataattca ccattgctg aatagggtc acggcgcaa 
      ggggagcag attttcatg accatctcg gcttatttg 
      gagttggaa aattgcggc cgccctgcc attataaca 
      aaagacctg agtatggta atctacgga agagacgct 
      cgaactggc cctcgatcc ctcaaacac ctcttctct 
      ggaaactat cgcaacgcc gaagcaaac cgcctatca 
      ggagtctac gaattactt ttgcgacgt gctagtgaa 
      gcaggtagt ccaggtgtg caaagacgc caacgtcgc 
      gtcctggac accagctcc acctacgtc cgcggcgag 
      gaaaacctc cacggttgg cgtcctcgt ggcgattcg 
      ttaatcttc gaccaccaa tgggagctc gagaaattg 
      acccgactt gaagaagtc gaacgtgtg cgccacact 
      ctcctcctc cgcgagcgt ctaggtctc gaccgttca 
      accaatcac aacaagtat cacctcgac tacacaaaa 
      agcgagaag gaagtgtgc aacatggtc gctaaaaac 
      aacaacctc aacgcaacc aacgatttt tacgaatac 
      accgatcgc gagaaacaa ctcttaatg aaatgcgtc 
      cgcttgatc cagggacga ggccgcgaa agcatcacc 
      ggaatgaag gaaaaagag gtggcaagt ccggaagaa 
      atgaccgaa atgagcacg agcatgcta tcaagcatg 
      gtgtcgaac gggtcgatc gagctctgc tcaccggaa 
      cgggcggcg cttccgggc gaatgtgcc ccgttcccg 
      ccggttccg gtctctccc ccaggcccc agggacccg 
      gagttggtg ctctacgtc ccggacatg gaggaaatt 
      aggatttcg cccgtcgtc gcacgcaag gggtatttg 
      aacgttttg gagcataaa acgcacggg tggaagaag 
      cgatgggtg gccgtccga cgaccctac gtgtttatt 
      ttccgggat gaaaaagat ccagttgaa cgggcgctt 
      attaaccta gctacggcc caagtggag tattccgag 
      gatcagttg gcgatggtt cgactcccg aatactttc 
      agcgtggtg agcaaacat cgcggttat ctccttcaa 
      acgcttcac gagaaggag gtgcacgat tggttgtat 
      gccattaat ccgttatta gctgggcaa attcgctcg 
      aaatcggcc agacgtaat ccaacacgt caggaaaat 
      acggaaact acggtggca tgtaaatga gatttaatc 
      gttatggaa tccgttttg gtttattag gtttttttg 
      agttgattg aggttgtga tagggttcc ataaacggg 
      ggatttggt tcttctagc ccaggtgtt gcgggattg 
      tttggcgaa atgattcgc gacacctgg gctgtttct 
      agtcgagca tttctttac aaatctagt cgaggaatt 
      gtgtgagaa tttttatga tccggttgt gtgcaataa 
      gagagtttc tcagaccgt ttcgaatgc ttatttcag 
      tttgattga tctcagtgt taaaatagt attaaagct 
      tcttatagt gtaatgaat atttaatac atgtaccaa 
      tctaattaa acatcatat tctaccgtt ttaatgtta 
      aggcctcgg gtttttctt tgacaaagt gaatgtaaa 
      gaaagcaga aatttcgac actttttcg tttgatttt 
      cgggcacag taaatgtat gatgtacag ggtgtttat 
      tgttcaatc gatgaagag ctcactttt tgattattt 
      ttggaaact tccacttca accaaccct gttgttatt 
      tttgtactt gaactagat ttacacgaa ttgttttta 
      ttgtatata cctacctac caaagttag cacaataga 
      tctcacgct gaaaccgct attgtttca aaactggca 
      tttgtaagc cgaagctta catccatga ccatgtctt 
      tgactgatt tttattcga tttggttgt tgtaggtgc 
      ttgttaaac tcatcataa ttttatttt tatagttag 
      ttcccctat agacacagt gctacgctt atctaagct 
      ataaatgta aaaaagaag tactattta atgaaatta 
      gtaggtaca atgtaggaa aatttactt tccttttct 
      ttattttat ttcggatgc tgcctttgc aggctcatt 
      attgattaa ttgaatgat tattattat tgtataatt 
      gtgtaagca ttatatgaa ataaataaa gaaaaccaa 
      aatat
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