| General information | |||
| TC006253 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 5839607..5866124 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006253 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04099 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
Days post injection
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC006253 RA
MSSVKVAVR VRPFNNREI SRECKCIIK MGGNTTTIT NPKADPKEA LKSFNFDYS YWSHDETDP EFSSQLVVY KDIGEEMLQ HSFDGYNVC IFAYGQTGA GKSYTMMGK QEEGQEGII PQICKDLFN RIRNNDNPD MKYSVEVSY MEIYCERVR DLLNPKNKG NLRVREHPL LGPYVEDLS KLAVTSYED IHDLIDEGN KARTVAATN MNETSSRSH AVFTIFFTQ QRYDQTTTL CTEKVSKIS LVDLAGSER ADSTGAKGT RLKEGANIN KSLTTLGKV ISALAEIAS KNKKSKKAD FIPYRDSVL TWLLRENLG GNSKTAMIA AISPADINY DETLSTLRY ADRAKQIVC KAVVNEDAN AKLIRELKE EIQKLRELL KAEGIEVHE GAEDNIIAR NNKENERSR TTSTTIAEE AVDQLQASE KLIAELNET WEEKLKRTE AIRIQREAV FAEMGVAVK EDGITVGVF SPKCTPHLV NLNEDPFMS ECLIYYTKD GITRVGSAE ANIPQDIQL VGSHIKSEH CIFENKDGK VTLTPSAGA LIYVNGREI TEPVVLKTG SRVILGKNH VFRFTHPDE AREMREKNS TPLETVDWN YAQIELLEK QGVDLKAEM EKRLLTLEE QYRKEKETA DQLFEEQRK NYEARIDAL QKQVEEQSM TMSMYSSYT PEDFNNEDD IFECNWTEK EYELAANAW RKWKHHQFT SLRDDLWGN AIFLKEANA ISVELKKKV QFQFTLLTD TLYSPLPAD LRPTIDYDQ DEDASVPKT IVAVEVQDT KNGATHYWS LDKLRQRLE LMRLVYNEE VVELPSPEV GIETTTPLL RCLTQCPPS PSRFSLTSL LPSRQRLEL MREMYHNEA EMSPTSPDY NVESLTGGD PFYDRFPWF RMVGRGFVY LSNLLYPVT LIHKVAIVS EKGDVRGYL RVAVQAVMD EDNSDQVGV RQSARIAFE TPKAVIEHK NIHNMDDRI IEGQSAIDP IKIDDLIEC DADSGRGDS SVSSDLKED EVPDHLAIG KEFTFRVTV LQAVGISTE YADIFCQFN FLHRHDEAF STEPVKNSG KGTPLGFYH VQNITVSTT KSFIDYIKT QPIVFEVFG HYQQHPLHK DAKLEGNVR QPPRRMLPP SIPISQPVR SSKFGALPS PCTAHIHAK VDVLVWFEI CELAPSGEY VPVVVEHSD DLPCRGLFL LHQGIQRRI RITIVHDQA AEIKWRDVR ELVVGRIRN SPESDEDED NDNSVLSLG LFPGEYLEV PGDDRVMFR FEAAWDSSL HNSPLLNRV TAQGEQIFM TISAYLELE NCGRPAIIT KDLSMVIYG RDARTGPRS LKHLFSGNY RNAEANRLS GVYELLLRR ASEAGSPGV QRRQRRVLD TSSTYVRGE ENLHGWRPR GDSLIFDHQ WELEKLTRL EEVERVRHT LLLRERLGL DRSTNHNKY HLDYTKSEK EVCNMVAKN NNLNATNDF YEYTDREKQ LLMKCVRLI QGRGRESIT GMKEKEVAS PEEMTEMST SMLSSMVSN GSIELCSPE RAALPGECA PFPPVPVSP PGPRDPELV LYVPDMEEI RISPVVARK GYLNVLEHK THGWKKRWV AVRRPYVFI FRDEKDPVE RALINLATA QVEYSEDQL AMVRLPNTF SVVSKHRGY LLQTLHEKE VHDWLYAIN PLLAGQIRS KSARRNPTR QENTETTVA CK
- >TC006253 RB
MSSVKVAVR VRPFNNREI SRECKCIIK MGGNTTTIT NPKADPKEA LKSFNFDYS YWSHDETDP EFSSQLVVY KDIGEEMLQ HSFDGYNVC IFAYGQTGA GKSYTMMGK QEEGQEGII PQICKDLFN RIRNNDNPD MKYSVEVSY MEIYCERVR DLLNPKNKG NLRVREHPL LGPYVEDLS KLAVTSYED IHDLIDEGN KARTVAATN MNETSSRSH AVFTIFFTQ QRYDQTTTL CTEKVSKIS LVDLAGSER ADSTGAKGT RLKEGANIN KSLTTLGKV ISALAEIAS KNKKSKKAD FIPYRDSVL TWLLRENLG GNSKTAMIA AISPADINY DETLSTLRY ADRAKQIVC KAVVNEDAN AKLIRELKE EIQKLRELL KAEGIEVHE GAEDNIIAR NNKENERSR TTSTTIAEE AVDQLQASE KLIAELNET WEEKLKRTE AIRIQREAV FAEMGVAVK EDGITVGVF SPKCTPHLV NLNEDPFMS ECLIYYTKD GITRVGSAE ANIPQDIQL VGSHIKSEH CIFENKDGK VTLTPSAGA LIYVNGREI TEPVVLKTG SRVILGKNH VFRFTHPDE AREMREKNS TPLETVDWN YAQIELLEK QGVDLKAEM EKRLLTLEE QYRKEKETA DQLFEEQRK NYEARIDAL QKQVEEQSM TMSMYSSYT PEDFNNEDD IFVNPLFDA ECNWTEKEY ELAANAWRK WKHHQFTSL RDDLWGNAI FLKEANAIS VELKKKVQF QFTLLTDTL YSPLPADLR PTIDYDQDE DASVPKTIV AVEVQDTKN GATHYWSLD KLRQRLELM RLVYNEEVV ELPSPEVGI ETTTPLLRC LTQCPPSPS RFSLTSLLP SRQRLELMR EMYHNEAEM SPTSPDYNV ESLTGGDPF YDRFPWFRM VGRGFVYLS NLLYPVTLI HKVAIVSEK GDVRGYLRV AVQAVMDED NSDQVGVRQ SARIAFETP KAVIEHKNI HNMDDRIIE GQSAIDPIK IDDLIECDA DSGRGDSSV SSDLKEDEV PDHLAIGKE FTFRVTVLQ AVGISTEYA DIFCQFNFL HRHDEAFST EPVKNSGKG TPLGFYHVQ NITVSTTKS FIDYIKTQP IVFEVFGHY QQHPLHKDA KLEGNVRQP PRRMLPPSI PISQPVRSS KFGALPSPC TAHIHAKVD VLVWFEICE LAPSGEYVP VVVEHSDDL PCRGLFLLH QGIQRRIRI TIVHDQAAE IKWRDVREL VVGRIRNSP ESDEDEDND NSVLSLGLF PGEYLEVPG DDRVMFRFE AAWDSSLHN SPLLNRVTA QGEQIFMTI SAYLELENC GRPAIITKD LSMVIYGRD ARTGPRSLK HLFSGNYRN AEANRLSGV YELLLRRAS EAGSPGVQR RQRRVLDTS STYVRGEEN LHGWRPRGD SLIFDHQWE LEKLTRLEE VERVRHTLL LRERLGLDR STNHNKYHL DYTKSEKEV CNMVAKNNN LNATNDFYE YTDREKQLL MKCVRLIQG RGRESITGM KEKEVASPE EMTEMSTSM LSSMVSNGS IELCSPERA ALPGECAPF PPVPVSPPG PRDPELVLY VPDMEEIRI SPVVARKGY LNVLEHKTH GWKKRWVAV RRPYVFIFR DEKDPVERA LINLATAQV EYSEDQLAM VRLPNTFSV VSKHRGYLL QTLHEKEVH DWLYAINPL LAGQIRSKS ARRNPTRQE NTETTVACK
- >TC006253 RA
atgtcgtca gtcaaagta gcagttcgt gtccggccg ttcaacaac cgagaaatc tcgcgagaa tgcaaatgt ataataaaa atgggagga aacactaca acaatcaca aatccaaaa gccgacccg aaagaagcc ctcaaaagc ttcaatttc gactattcc tactggtca cacgatgag accgacccg gaattctcc tcacaactg gtcgtctac aaggacatc ggcgaggaa atgctccag cactccttc gacggctac aacgtttgc atcttcgcc tacgggcag accggcgcc ggcaagtcc tacaccatg atgggcaag caggaggag ggccaagaa ggcatcatc ccccagatc tgcaaggac ctcttcaac aggatacgc aacaacgac aaccccgac atgaagtac tcggtggag gtcagctac atggagatc tactgcgag cgggtgcgc gatctgctc aaccccaag aacaagggg aacttgcgg gtgcgcgag caccccctt ctggggccc tacgtcgag gatctgagc aagctggcg gtgacgagc tacgaggac atccacgac ctgatagat gagggcaac aaggccaga acggtggcc gccactaac atgaacgag accagttcg aggtcgcat gcggtgttt acgattttt ttcacgcaa cagagatac gatcaaacg acgacattg tgcacggag aaggtctcc aagatctcg ctggtggac ctggcgggc tccgagagg gccgactcc acgggggcc aagggcacg aggctcaag gagggcgcc aacatcaac aagagtctg acgacgttg ggcaaggtc atctcggcg ctcgctgaa atcgcgtct aagaacaag aaatcaaaa aaagccgac tttatccct taccgagac tcggttttg acatggttg ctgcgcgag aacctcgga ggcaacagc aagactgca atgatagcc gccatatct ccagccgat atcaactac gacgagact ttgtccact ctcaggtac gctgatcga gccaagcag atcgtgtgc aaggccgtg gttaacgag gacgccaac gcgaaactc atccgagag ctcaaagag gagatccag aaactccgc gagctgctc aaggccgaa ggcatcgaa gtgcacgag ggagctgaa gataacatc atcgctcgc aacaataaa gaaaacgag agatcgaga acgacttca acgacaata gccgaggaa gccgtcgat caattgcaa gccagcgag aaactcatc gccgaactg aacgaaaca tgggaggag aaattgaag cgcaccgaa gctataaga atccaacgg gaggccgtt ttcgccgaa atgggggtg gcggtgaag gaggacggg atcacggtt ggggtgttc tcccccaaa tgtaccccc catttggtg aatttgaac gaggatccg ttcatgtcc gaatgcctg atttactac accaaggac ggtattacg agggtcggg tcagccgag gcaaacatt ccgcaagat atacagctc gtggggtcg cacattaaa tcagagcat tgcattttt gagaataag gacgggaaa gtgacactg actccatca gctggggct ttaatttat gtcaatgga cgggagatc accgagcca gtcgtcctc aaaaccggc tcccgagtc attttgggc aaaaaccac gtgttccgc ttcacgcat ccagacgaa gcgcgtgag atgcgtgaa aaaaattcg acaccttta gaaaccgtt gactggaac tatgcacaa attgaactt cttgaaaaa caaggcgtt gacttaaag gccgagatg gaaaaacgt ctcctaacc ctcgaagaa caataccgt aaggaaaaa gaaaccgca gatcaactg tttgaagag cagcgaaaa aattacgag gctcgaatt gacgcgctt caaaagcaa gtggaagaa cagagcatg acaatgtcg atgtacagt tcctatacg cccgaggat ttcaataat gaagatgat attttcgag tgcaattgg accgagaag gagtacgag ctggccgcc aatgcctgg aggaagtgg aaacatcac cagtttacc tccttgagg gacgatctc tggggcaat gccattttc ctcaaggag gcgaacgcc atttcggtc gaactcaag aagaaagta caattccaa ttcacactt ttgaccgac accctctac tcgcccctt ccggccgat ttgcgtccc accatcgac tatgatcaa gacgaggac gcgtcagtg ccgaaaaca attgtcgct gttgaagtt caagatacg aaaaacggg gcgactcat tactggtcg ttggataaa cttcgtcaa agactggaa ttgatgcgt ctggtttat aacgaggaa gttgtggaa ttgccgagt cctgaagtt ggtattgaa acaacaacg cctttgctg agatgtctc acgcaatgt ccaccatca ccgtcacgc ttctctctt accagtctg ttgccgtcg aggcaaagg ctggaactc atgcgcgaa atgtaccac aacgaggcc gaaatgtca ccgacttcg cccgattat aatgtggag tcgctcact gggggcgac cccttctat gaccgtttt ccctggttt agaatggtg gggcgaggg tttgtctac ttgagcaat cttttatac cccgtgacg ctgattcat aaagtggcg attgttagt gaaaaagga gatgttcga ggatatttg agagtagcg gttcaggcc gttatggat gaggataat tcggaccaa gttggtgtg aggcaaagt gcaaggata gcttttgaa acacccaaa gccgttatt gaacataaa aatattcac aatatggac gatcgcatt atcgaaggt cagagcgct attgatccg attaaaatc gatgatctt atagagtgt gatgcagat tcaggacgt ggcgatagt tccgtttcg tcagattta aaagaggat gaagttccc gatcattta gcgatcgga aaagagttt actttcaga gttactgtc ttacaagca gtgggaatt tccacggaa tatgccgat atcttctgc caattcaat ttcttgcat agacatgac gaggctttt tcgacagaa ccagtcaaa aattcgggc aaaggcact ccacttggc ttctaccac gtacaaaat atcacagtt tcgaccacg aaatcattc atcgactat atcaaaacc cagccaatt gtcttcgaa gtttttggc cactaccaa cagcacccc cttcacaaa gacgccaaa ttggagggc aacgtccgc cagccccct cgccgcatg ctcccgccc tccatcccc atttcgcag ccagtcaga tcgagcaaa ttcggcgct ttgccctcc ccctgcacc gctcacatt cacgccaaa gtggacgtt ttagtttgg tttgaaatc tgcgaattg gccccgagt ggcgaatac gtccctgtt gtggtggag cacagtgat gatttgcct tgtcgtgga ttgtttttg ttgcatcag gggattcaa agacggatt aggattaca attgttcat gatcaagcc gctgagatt aaatggaga gatgtgaga gagttggta gtgggaagg attaggaat agtccggag tcggacgag gatgaggat aatgataat tcggtgctg tcgttggga ttattccca ggggagtat ctggaggtg cctggggat gatagggtg atgtttagg tttgaggcg gcctgggat agctcgctg cataattca ccattgctg aatagggtc acggcgcaa ggggagcag attttcatg accatctcg gcttatttg gagttggaa aattgcggc cgccctgcc attataaca aaagacctg agtatggta atctacgga agagacgct cgaactggc cctcgatcc ctcaaacac ctcttctct ggaaactat cgcaacgcc gaagcaaac cgcctatca ggagtctac gaattactt ttgcgacgt gctagtgaa gcaggtagt ccaggtgtg caaagacgc caacgtcgc gtcctggac accagctcc acctacgtc cgcggcgag gaaaacctc cacggttgg cgtcctcgt ggcgattcg ttaatcttc gaccaccaa tgggagctc gagaaattg acccgactt gaagaagtc gaacgtgtg cgccacact ctcctcctc cgcgagcgt ctaggtctc gaccgttca accaatcac aacaagtat cacctcgac tacacaaaa agcgagaag gaagtgtgc aacatggtc gctaaaaac aacaacctc aacgcaacc aacgatttt tacgaatac accgatcgc gagaaacaa ctcttaatg aaatgcgtc cgcttgatc cagggacga ggccgcgaa agcatcacc ggaatgaag gaaaaagag gtggcaagt ccggaagaa atgaccgaa atgagcacg agcatgcta tcaagcatg gtgtcgaac gggtcgatc gagctctgc tcaccggaa cgggcggcg cttccgggc gaatgtgcc ccgttcccg ccggttccg gtctctccc ccaggcccc agggacccg gagttggtg ctctacgtc ccggacatg gaggaaatt aggatttcg cccgtcgtc gcacgcaag gggtatttg aacgttttg gagcataaa acgcacggg tggaagaag cgatgggtg gccgtccga cgaccctac gtgtttatt ttccgggat gaaaaagat ccagttgaa cgggcgctt attaaccta gctacggcc caagtggag tattccgag gatcagttg gcgatggtt cgactcccg aatactttc agcgtggtg agcaaacat cgcggttat ctccttcaa acgcttcac gagaaggag gtgcacgat tggttgtat gccattaat ccgttatta gctgggcaa attcgctcg aaatcggcc agacgtaat ccaacacgt caggaaaat acggaaact acggtggca tgtaaatga
- >TC006253 RB
atgtcgtca gtcaaagta gcagttcgt gtccggccg ttcaacaac cgagaaatc tcgcgagaa tgcaaatgt ataataaaa atgggagga aacactaca acaatcaca aatccaaaa gccgacccg aaagaagcc ctcaaaagc ttcaatttc gactattcc tactggtca cacgatgag accgacccg gaattctcc tcacaactg gtcgtctac aaggacatc ggcgaggaa atgctccag cactccttc gacggctac aacgtttgc atcttcgcc tacgggcag accggcgcc ggcaagtcc tacaccatg atgggcaag caggaggag ggccaagaa ggcatcatc ccccagatc tgcaaggac ctcttcaac aggatacgc aacaacgac aaccccgac atgaagtac tcggtggag gtcagctac atggagatc tactgcgag cgggtgcgc gatctgctc aaccccaag aacaagggg aacttgcgg gtgcgcgag caccccctt ctggggccc tacgtcgag gatctgagc aagctggcg gtgacgagc tacgaggac atccacgac ctgatagat gagggcaac aaggccaga acggtggcc gccactaac atgaacgag accagttcg aggtcgcat gcggtgttt acgattttt ttcacgcaa cagagatac gatcaaacg acgacattg tgcacggag aaggtctcc aagatctcg ctggtggac ctggcgggc tccgagagg gccgactcc acgggggcc aagggcacg aggctcaag gagggcgcc aacatcaac aagagtctg acgacgttg ggcaaggtc atctcggcg ctcgctgaa atcgcgtct aagaacaag aaatcaaaa aaagccgac tttatccct taccgagac tcggttttg acatggttg ctgcgcgag aacctcgga ggcaacagc aagactgca atgatagcc gccatatct ccagccgat atcaactac gacgagact ttgtccact ctcaggtac gctgatcga gccaagcag atcgtgtgc aaggccgtg gttaacgag gacgccaac gcgaaactc atccgagag ctcaaagag gagatccag aaactccgc gagctgctc aaggccgaa ggcatcgaa gtgcacgag ggagctgaa gataacatc atcgctcgc aacaataaa gaaaacgag agatcgaga acgacttca acgacaata gccgaggaa gccgtcgat caattgcaa gccagcgag aaactcatc gccgaactg aacgaaaca tgggaggag aaattgaag cgcaccgaa gctataaga atccaacgg gaggccgtt ttcgccgaa atgggggtg gcggtgaag gaggacggg atcacggtt ggggtgttc tcccccaaa tgtaccccc catttggtg aatttgaac gaggatccg ttcatgtcc gaatgcctg atttactac accaaggac ggtattacg agggtcggg tcagccgag gcaaacatt ccgcaagat atacagctc gtggggtcg cacattaaa tcagagcat tgcattttt gagaataag gacgggaaa gtgacactg actccatca gctggggct ttaatttat gtcaatgga cgggagatc accgagcca gtcgtcctc aaaaccggc tcccgagtc attttgggc aaaaaccac gtgttccgc ttcacgcat ccagacgaa gcgcgtgag atgcgtgaa aaaaattcg acaccttta gaaaccgtt gactggaac tatgcacaa attgaactt cttgaaaaa caaggcgtt gacttaaag gccgagatg gaaaaacgt ctcctaacc ctcgaagaa caataccgt aaggaaaaa gaaaccgca gatcaactg tttgaagag cagcgaaaa aattacgag gctcgaatt gacgcgctt caaaagcaa gtggaagaa cagagcatg acaatgtcg atgtacagt tcctatacg cccgaggat ttcaataat gaagatgat attttcgtg aaccctctg tttgacgca gagtgcaat tggaccgag aaggagtac gagctggcc gccaatgcc tggaggaag tggaaacat caccagttt acctccttg agggacgat ctctggggc aatgccatt ttcctcaag gaggcgaac gccatttcg gtcgaactc aagaagaaa gtacaattc caattcaca cttttgacc gacaccctc tactcgccc cttccggcc gatttgcgt cccaccatc gactatgat caagacgag gacgcgtca gtgccgaaa acaattgtc gctgttgaa gttcaagat acgaaaaac ggggcgact cattactgg tcgttggat aaacttcgt caaagactg gaattgatg cgtctggtt tataacgag gaagttgtg gaattgccg agtcctgaa gttggtatt gaaacaaca acgcctttg ctgagatgt ctcacgcaa tgtccacca tcaccgtca cgcttctct cttaccagt ctgttgccg tcgaggcaa aggctggaa ctcatgcgc gaaatgtac cacaacgag gccgaaatg tcaccgact tcgcccgat tataatgtg gagtcgctc actgggggc gaccccttc tatgaccgt tttccctgg tttagaatg gtggggcga gggtttgtc tacttgagc aatctttta taccccgtg acgctgatt cataaagtg gcgattgtt agtgaaaaa ggagatgtt cgaggatat ttgagagta gcggttcag gccgttatg gatgaggat aattcggac caagttggt gtgaggcaa agtgcaagg atagctttt gaaacaccc aaagccgtt attgaacat aaaaatatt cacaatatg gacgatcgc attatcgaa ggtcagagc gctattgat ccgattaaa atcgatgat cttatagag tgtgatgca gattcagga cgtggcgat agttccgtt tcgtcagat ttaaaagag gatgaagtt cccgatcat ttagcgatc ggaaaagag tttactttc agagttact gtcttacaa gcagtggga atttccacg gaatatgcc gatatcttc tgccaattc aatttcttg catagacat gacgaggct ttttcgaca gaaccagtc aaaaattcg ggcaaaggc actccactt ggcttctac cacgtacaa aatatcaca gtttcgacc acgaaatca ttcatcgac tatatcaaa acccagcca attgtcttc gaagttttt ggccactac caacagcac ccccttcac aaagacgcc aaattggag ggcaacgtc cgccagccc cctcgccgc atgctcccg ccctccatc cccatttcg cagccagtc agatcgagc aaattcggc gctttgccc tccccctgc accgctcac attcacgcc aaagtggac gttttagtt tggtttgaa atctgcgaa ttggccccg agtggcgaa tacgtccct gttgtggtg gagcacagt gatgatttg ccttgtcgt ggattgttt ttgttgcat caggggatt caaagacgg attaggatt acaattgtt catgatcaa gccgctgag attaaatgg agagatgtg agagagttg gtagtggga aggattagg aatagtccg gagtcggac gaggatgag gataatgat aattcggtg ctgtcgttg ggattattc ccaggggag tatctggag gtgcctggg gatgatagg gtgatgttt aggtttgag gcggcctgg gatagctcg ctgcataat tcaccattg ctgaatagg gtcacggcg caaggggag cagattttc atgaccatc tcggcttat ttggagttg gaaaattgc ggccgccct gccattata acaaaagac ctgagtatg gtaatctac ggaagagac gctcgaact ggccctcga tccctcaaa cacctcttc tctggaaac tatcgcaac gccgaagca aaccgccta tcaggagtc tacgaatta cttttgcga cgtgctagt gaagcaggt agtccaggt gtgcaaaga cgccaacgt cgcgtcctg gacaccagc tccacctac gtccgcggc gaggaaaac ctccacggt tggcgtcct cgtggcgat tcgttaatc ttcgaccac caatgggag ctcgagaaa ttgacccga cttgaagaa gtcgaacgt gtgcgccac actctcctc ctccgcgag cgtctaggt ctcgaccgt tcaaccaat cacaacaag tatcacctc gactacaca aaaagcgag aaggaagtg tgcaacatg gtcgctaaa aacaacaac ctcaacgca accaacgat ttttacgaa tacaccgat cgcgagaaa caactctta atgaaatgc gtccgcttg atccaggga cgaggccgc gaaagcatc accggaatg aaggaaaaa gaggtggca agtccggaa gaaatgacc gaaatgagc acgagcatg ctatcaagc atggtgtcg aacgggtcg atcgagctc tgctcaccg gaacgggcg gcgcttccg ggcgaatgt gccccgttc ccgccggtt ccggtctct cccccaggc cccagggac ccggagttg gtgctctac gtcccggac atggaggaa attaggatt tcgcccgtc gtcgcacgc aaggggtat ttgaacgtt ttggagcat aaaacgcac gggtggaag aagcgatgg gtggccgtc cgacgaccc tacgtgttt attttccgg gatgaaaaa gatccagtt gaacgggcg cttattaac ctagctacg gcccaagtg gagtattcc gaggatcag ttggcgatg gttcgactc ccgaatact ttcagcgtg gtgagcaaa catcgcggt tatctcctt caaacgctt cacgagaag gaggtgcac gattggttg tatgccatt aatccgtta ttagctggg caaattcgc tcgaaatcg gccagacgt aatccaaca cgtcaggaa aatacggaa actacggtg gcatgtaaa tga
- >TC006253 RA
ttattaaca aatgtcatg tagtgtttg tttctgcaa gaaaaatcg taaataaag acaataagt tttagggaa acataaata tcaaacatg tcgtcagtc aaagtagca gttcgtgtc cggccgttc aacaaccga gaaatctcg cgagaatgc aaatgtata ataaaaatg ggaggaaac actacaaca atcacaaat ccaaaagcc gacccgaaa gaagccctc aaaagcttc aatttcgac tattcctac tggtcacac gatgagacc gacccggaa ttctcctca caactggtc gtctacaag gacatcggc gaggaaatg ctccagcac tccttcgac ggctacaac gtttgcatc ttcgcctac gggcagacc ggcgccggc aagtcctac accatgatg ggcaagcag gaggagggc caagaaggc atcatcccc cagatctgc aaggacctc ttcaacagg atacgcaac aacgacaac cccgacatg aagtactcg gtggaggtc agctacatg gagatctac tgcgagcgg gtgcgcgat ctgctcaac cccaagaac aaggggaac ttgcgggtg cgcgagcac ccccttctg gggccctac gtcgaggat ctgagcaag ctggcggtg acgagctac gaggacatc cacgacctg atagatgag ggcaacaag gccagaacg gtggccgcc actaacatg aacgagacc agttcgagg tcgcatgcg gtgtttacg atttttttc acgcaacag agatacgat caaacgacg acattgtgc acggagaag gtctccaag atctcgctg gtggacctg gcgggctcc gagagggcc gactccacg ggggccaag ggcacgagg ctcaaggag ggcgccaac atcaacaag agtctgacg acgttgggc aaggtcatc tcggcgctc gctgaaatc gcgtctaag aacaagaaa tcaaaaaaa gccgacttt atcccttac cgagactcg gttttgaca tggttgctg cgcgagaac ctcggaggc aacagcaag actgcaatg atagccgcc atatctcca gccgatatc aactacgac gagactttg tccactctc aggtacgct gatcgagcc aagcagatc gtgtgcaag gccgtggtt aacgaggac gccaacgcg aaactcatc cgagagctc aaagaggag atccagaaa ctccgcgag ctgctcaag gccgaaggc atcgaagtg cacgaggga gctgaagat aacatcatc gctcgcaac aataaagaa aacgagaga tcgagaacg acttcaacg acaatagcc gaggaagcc gtcgatcaa ttgcaagcc agcgagaaa ctcatcgcc gaactgaac gaaacatgg gaggagaaa ttgaagcgc accgaagct ataagaatc caacgggag gccgttttc gccgaaatg ggggtggcg gtgaaggag gacgggatc acggttggg gtgttctcc cccaaatgt accccccat ttggtgaat ttgaacgag gatccgttc atgtccgaa tgcctgatt tactacacc aaggacggt attacgagg gtcgggtca gccgaggca aacattccg caagatata cagctcgtg gggtcgcac attaaatca gagcattgc atttttgag aataaggac gggaaagtg acactgact ccatcagct ggggcttta atttatgtc aatggacgg gagatcacc gagccagtc gtcctcaaa accggctcc cgagtcatt ttgggcaaa aaccacgtg ttccgcttc acgcatcca gacgaagcg cgtgagatg cgtgaaaaa aattcgaca cctttagaa accgttgac tggaactat gcacaaatt gaacttctt gaaaaacaa ggcgttgac ttaaaggcc gagatggaa aaacgtctc ctaaccctc gaagaacaa taccgtaag gaaaaagaa accgcagat caactgttt gaagagcag cgaaaaaat tacgaggct cgaattgac gcgcttcaa aagcaagtg gaagaacag agcatgaca atgtcgatg tacagttcc tatacgccc gaggatttc aataatgaa gatgatatt ttcgagtgc aattggacc gagaaggag tacgagctg gccgccaat gcctggagg aagtggaaa catcaccag tttacctcc ttgagggac gatctctgg ggcaatgcc attttcctc aaggaggcg aacgccatt tcggtcgaa ctcaagaag aaagtacaa ttccaattc acacttttg accgacacc ctctactcg ccccttccg gccgatttg cgtcccacc atcgactat gatcaagac gaggacgcg tcagtgccg aaaacaatt gtcgctgtt gaagttcaa gatacgaaa aacggggcg actcattac tggtcgttg gataaactt cgtcaaaga ctggaattg atgcgtctg gtttataac gaggaagtt gtggaattg ccgagtcct gaagttggt attgaaaca acaacgcct ttgctgaga tgtctcacg caatgtcca ccatcaccg tcacgcttc tctcttacc agtctgttg ccgtcgagg caaaggctg gaactcatg cgcgaaatg taccacaac gaggccgaa atgtcaccg acttcgccc gattataat gtggagtcg ctcactggg ggcgacccc ttctatgac cgttttccc tggtttaga atggtgggg cgagggttt gtctacttg agcaatctt ttatacccc gtgacgctg attcataaa gtggcgatt gttagtgaa aaaggagat gttcgagga tatttgaga gtagcggtt caggccgtt atggatgag gataattcg gaccaagtt ggtgtgagg caaagtgca aggatagct tttgaaaca cccaaagcc gttattgaa cataaaaat attcacaat atggacgat cgcattatc gaaggtcag agcgctatt gatccgatt aaaatcgat gatcttata gagtgtgat gcagattca ggacgtggc gatagttcc gtttcgtca gatttaaaa gaggatgaa gttcccgat catttagcg atcggaaaa gagtttact ttcagagtt actgtctta caagcagtg ggaatttcc acggaatat gccgatatc ttctgccaa ttcaatttc ttgcataga catgacgag gctttttcg acagaacca gtcaaaaat tcgggcaaa ggcactcca cttggcttc taccacgta caaaatatc acagtttcg accacgaaa tcattcatc gactatatc aaaacccag ccaattgtc ttcgaagtt tttggccac taccaacag caccccctt cacaaagac gccaaattg gagggcaac gtccgccag ccccctcgc cgcatgctc ccgccctcc atccccatt tcgcagcca gtcagatcg agcaaattc ggcgctttg ccctccccc tgcaccgct cacattcac gccaaagtg gacgtttta gtttggttt gaaatctgc gaattggcc ccgagtggc gaatacgtc cctgttgtg gtggagcac agtgatgat ttgccttgt cgtggattg tttttgttg catcagggg attcaaaga cggattagg attacaatt gttcatgat caagccgct gagattaaa tggagagat gtgagagag ttggtagtg ggaaggatt aggaatagt ccggagtcg gacgaggat gaggataat gataattcg gtgctgtcg ttgggatta ttcccaggg gagtatctg gaggtgcct ggggatgat agggtgatg tttaggttt gaggcggcc tgggatagc tcgctgcat aattcacca ttgctgaat agggtcacg gcgcaaggg gagcagatt ttcatgacc atctcggct tatttggag ttggaaaat tgcggccgc cctgccatt ataacaaaa gacctgagt atggtaatc tacggaaga gacgctcga actggccct cgatccctc aaacacctc ttctctgga aactatcgc aacgccgaa gcaaaccgc ctatcagga gtctacgaa ttacttttg cgacgtgct agtgaagca ggtagtcca ggtgtgcaa agacgccaa cgtcgcgtc ctggacacc agctccacc tacgtccgc ggcgaggaa aacctccac ggttggcgt cctcgtggc gattcgtta atcttcgac caccaatgg gagctcgag aaattgacc cgacttgaa gaagtcgaa cgtgtgcgc cacactctc ctcctccgc gagcgtcta ggtctcgac cgttcaacc aatcacaac aagtatcac ctcgactac acaaaaagc gagaaggaa gtgtgcaac atggtcgct aaaaacaac aacctcaac gcaaccaac gatttttac gaatacacc gatcgcgag aaacaactc ttaatgaaa tgcgtccgc ttgatccag ggacgaggc cgcgaaagc atcaccgga atgaaggaa aaagaggtg gcaagtccg gaagaaatg accgaaatg agcacgagc atgctatca agcatggtg tcgaacggg tcgatcgag ctctgctca ccggaacgg gcggcgctt ccgggcgaa tgtgccccg ttcccgccg gttccggtc tctccccca ggccccagg gacccggag ttggtgctc tacgtcccg gacatggag gaaattagg atttcgccc gtcgtcgca cgcaagggg tatttgaac gttttggag cataaaacg cacgggtgg aagaagcga tgggtggcc gtccgacga ccctacgtg tttattttc cgggatgaa aaagatcca gttgaacgg gcgcttatt aacctagct acggcccaa gtggagtat tccgaggat cagttggcg atggttcga ctcccgaat actttcagc gtggtgagc aaacatcgc ggttatctc cttcaaacg cttcacgag aaggaggtg cacgattgg ttgtatgcc attaatccg ttattagct gggcaaatt cgctcgaaa tcggccaga cgtaatcca acacgtcag gaaaatacg gaaactacg gtggcatgt aaatgagat ttaatcgtt atggaatcc gttttggtt tattaggtt tttttgagt tgattgagg ttgtgatag ggttccata aacggggga tttggttct tctagccca ggtgttgcg ggattgttt ggcgaaatg attcgcgac acctgggct gtttctagt cgagcattt ctttacaaa tctagtcga ggaattgtg tgagaattt ttatgatcc ggttgtgtg caataagag agtttctca gaccgtttc gaatgctta tttcagttt gattgatct cagtgttaa aatagtatt aaagcttct tatagtgta atgaatatt taatacatg taccaatct aattaaaca tcatattct accgtttta atgttaagg cctcgggtt tttctttga caaagtgaa tgtaaagaa agcagaaat ttcgacact ttttcgttt gattttcgg gcacagtaa atgtatgat gtacagggt gtttattgt tcaatcgat gaagagctc actttttga ttatttttg gaaacttcc acttcaacc aaccctgtt gttattttt gtacttgaa ctagattta cacgaattg tttttattg tatatacct acctaccaa agttagcac aatagatct cacgctgaa accgctatt gtttcaaaa ctggcattt gtaagccga agcttacat ccatgacca tgtctttga ctgattttt attcgattt ggttgttgt aggtgcttg ttaaactca tcataattt tatttttat agttagttc ccctataga cacagtgct acgcttatc taagctata aatgtaaaa aagaagtac tatttaatg aaattagta ggtacaatg taggaaaat ttactttcc ttttcttta ttttatttc ggatgctgc ctttgcagg ctcattatt gattaattg aatgattat tattattgt ataattgtg taagcatta tatgaaata aataaagaa aaccaaaat at
- >TC006253 RB
ttattaaca aatgtcatg tagtgtttg tttctgcaa gaaaaatcg taaataaag acaataagt tttagggaa acataaata tcaaacatg tcgtcagtc aaagtagca gttcgtgtc cggccgttc aacaaccga gaaatctcg cgagaatgc aaatgtata ataaaaatg ggaggaaac actacaaca atcacaaat ccaaaagcc gacccgaaa gaagccctc aaaagcttc aatttcgac tattcctac tggtcacac gatgagacc gacccggaa ttctcctca caactggtc gtctacaag gacatcggc gaggaaatg ctccagcac tccttcgac ggctacaac gtttgcatc ttcgcctac gggcagacc ggcgccggc aagtcctac accatgatg ggcaagcag gaggagggc caagaaggc atcatcccc cagatctgc aaggacctc ttcaacagg atacgcaac aacgacaac cccgacatg aagtactcg gtggaggtc agctacatg gagatctac tgcgagcgg gtgcgcgat ctgctcaac cccaagaac aaggggaac ttgcgggtg cgcgagcac ccccttctg gggccctac gtcgaggat ctgagcaag ctggcggtg acgagctac gaggacatc cacgacctg atagatgag ggcaacaag gccagaacg gtggccgcc actaacatg aacgagacc agttcgagg tcgcatgcg gtgtttacg atttttttc acgcaacag agatacgat caaacgacg acattgtgc acggagaag gtctccaag atctcgctg gtggacctg gcgggctcc gagagggcc gactccacg ggggccaag ggcacgagg ctcaaggag ggcgccaac atcaacaag agtctgacg acgttgggc aaggtcatc tcggcgctc gctgaaatc gcgtctaag aacaagaaa tcaaaaaaa gccgacttt atcccttac cgagactcg gttttgaca tggttgctg cgcgagaac ctcggaggc aacagcaag actgcaatg atagccgcc atatctcca gccgatatc aactacgac gagactttg tccactctc aggtacgct gatcgagcc aagcagatc gtgtgcaag gccgtggtt aacgaggac gccaacgcg aaactcatc cgagagctc aaagaggag atccagaaa ctccgcgag ctgctcaag gccgaaggc atcgaagtg cacgaggga gctgaagat aacatcatc gctcgcaac aataaagaa aacgagaga tcgagaacg acttcaacg acaatagcc gaggaagcc gtcgatcaa ttgcaagcc agcgagaaa ctcatcgcc gaactgaac gaaacatgg gaggagaaa ttgaagcgc accgaagct ataagaatc caacgggag gccgttttc gccgaaatg ggggtggcg gtgaaggag gacgggatc acggttggg gtgttctcc cccaaatgt accccccat ttggtgaat ttgaacgag gatccgttc atgtccgaa tgcctgatt tactacacc aaggacggt attacgagg gtcgggtca gccgaggca aacattccg caagatata cagctcgtg gggtcgcac attaaatca gagcattgc atttttgag aataaggac gggaaagtg acactgact ccatcagct ggggcttta atttatgtc aatggacgg gagatcacc gagccagtc gtcctcaaa accggctcc cgagtcatt ttgggcaaa aaccacgtg ttccgcttc acgcatcca gacgaagcg cgtgagatg cgtgaaaaa aattcgaca cctttagaa accgttgac tggaactat gcacaaatt gaacttctt gaaaaacaa ggcgttgac ttaaaggcc gagatggaa aaacgtctc ctaaccctc gaagaacaa taccgtaag gaaaaagaa accgcagat caactgttt gaagagcag cgaaaaaat tacgaggct cgaattgac gcgcttcaa aagcaagtg gaagaacag agcatgaca atgtcgatg tacagttcc tatacgccc gaggatttc aataatgaa gatgatatt ttcgtgaac cctctgttt gacgcagag tgcaattgg accgagaag gagtacgag ctggccgcc aatgcctgg aggaagtgg aaacatcac cagtttacc tccttgagg gacgatctc tggggcaat gccattttc ctcaaggag gcgaacgcc atttcggtc gaactcaag aagaaagta caattccaa ttcacactt ttgaccgac accctctac tcgcccctt ccggccgat ttgcgtccc accatcgac tatgatcaa gacgaggac gcgtcagtg ccgaaaaca attgtcgct gttgaagtt caagatacg aaaaacggg gcgactcat tactggtcg ttggataaa cttcgtcaa agactggaa ttgatgcgt ctggtttat aacgaggaa gttgtggaa ttgccgagt cctgaagtt ggtattgaa acaacaacg cctttgctg agatgtctc acgcaatgt ccaccatca ccgtcacgc ttctctctt accagtctg ttgccgtcg aggcaaagg ctggaactc atgcgcgaa atgtaccac aacgaggcc gaaatgtca ccgacttcg cccgattat aatgtggag tcgctcact gggggcgac cccttctat gaccgtttt ccctggttt agaatggtg gggcgaggg tttgtctac ttgagcaat cttttatac cccgtgacg ctgattcat aaagtggcg attgttagt gaaaaagga gatgttcga ggatatttg agagtagcg gttcaggcc gttatggat gaggataat tcggaccaa gttggtgtg aggcaaagt gcaaggata gcttttgaa acacccaaa gccgttatt gaacataaa aatattcac aatatggac gatcgcatt atcgaaggt cagagcgct attgatccg attaaaatc gatgatctt atagagtgt gatgcagat tcaggacgt ggcgatagt tccgtttcg tcagattta aaagaggat gaagttccc gatcattta gcgatcgga aaagagttt actttcaga gttactgtc ttacaagca gtgggaatt tccacggaa tatgccgat atcttctgc caattcaat ttcttgcat agacatgac gaggctttt tcgacagaa ccagtcaaa aattcgggc aaaggcact ccacttggc ttctaccac gtacaaaat atcacagtt tcgaccacg aaatcattc atcgactat atcaaaacc cagccaatt gtcttcgaa gtttttggc cactaccaa cagcacccc cttcacaaa gacgccaaa ttggagggc aacgtccgc cagccccct cgccgcatg ctcccgccc tccatcccc atttcgcag ccagtcaga tcgagcaaa ttcggcgct ttgccctcc ccctgcacc gctcacatt cacgccaaa gtggacgtt ttagtttgg tttgaaatc tgcgaattg gccccgagt ggcgaatac gtccctgtt gtggtggag cacagtgat gatttgcct tgtcgtgga ttgtttttg ttgcatcag gggattcaa agacggatt aggattaca attgttcat gatcaagcc gctgagatt aaatggaga gatgtgaga gagttggta gtgggaagg attaggaat agtccggag tcggacgag gatgaggat aatgataat tcggtgctg tcgttggga ttattccca ggggagtat ctggaggtg cctggggat gatagggtg atgtttagg tttgaggcg gcctgggat agctcgctg cataattca ccattgctg aatagggtc acggcgcaa ggggagcag attttcatg accatctcg gcttatttg gagttggaa aattgcggc cgccctgcc attataaca aaagacctg agtatggta atctacgga agagacgct cgaactggc cctcgatcc ctcaaacac ctcttctct ggaaactat cgcaacgcc gaagcaaac cgcctatca ggagtctac gaattactt ttgcgacgt gctagtgaa gcaggtagt ccaggtgtg caaagacgc caacgtcgc gtcctggac accagctcc acctacgtc cgcggcgag gaaaacctc cacggttgg cgtcctcgt ggcgattcg ttaatcttc gaccaccaa tgggagctc gagaaattg acccgactt gaagaagtc gaacgtgtg cgccacact ctcctcctc cgcgagcgt ctaggtctc gaccgttca accaatcac aacaagtat cacctcgac tacacaaaa agcgagaag gaagtgtgc aacatggtc gctaaaaac aacaacctc aacgcaacc aacgatttt tacgaatac accgatcgc gagaaacaa ctcttaatg aaatgcgtc cgcttgatc cagggacga ggccgcgaa agcatcacc ggaatgaag gaaaaagag gtggcaagt ccggaagaa atgaccgaa atgagcacg agcatgcta tcaagcatg gtgtcgaac gggtcgatc gagctctgc tcaccggaa cgggcggcg cttccgggc gaatgtgcc ccgttcccg ccggttccg gtctctccc ccaggcccc agggacccg gagttggtg ctctacgtc ccggacatg gaggaaatt aggatttcg cccgtcgtc gcacgcaag gggtatttg aacgttttg gagcataaa acgcacggg tggaagaag cgatgggtg gccgtccga cgaccctac gtgtttatt ttccgggat gaaaaagat ccagttgaa cgggcgctt attaaccta gctacggcc caagtggag tattccgag gatcagttg gcgatggtt cgactcccg aatactttc agcgtggtg agcaaacat cgcggttat ctccttcaa acgcttcac gagaaggag gtgcacgat tggttgtat gccattaat ccgttatta gctgggcaa attcgctcg aaatcggcc agacgtaat ccaacacgt caggaaaat acggaaact acggtggca tgtaaatga gatttaatc gttatggaa tccgttttg gtttattag gtttttttg agttgattg aggttgtga tagggttcc ataaacggg ggatttggt tcttctagc ccaggtgtt gcgggattg tttggcgaa atgattcgc gacacctgg gctgtttct agtcgagca tttctttac aaatctagt cgaggaatt gtgtgagaa tttttatga tccggttgt gtgcaataa gagagtttc tcagaccgt ttcgaatgc ttatttcag tttgattga tctcagtgt taaaatagt attaaagct tcttatagt gtaatgaat atttaatac atgtaccaa tctaattaa acatcatat tctaccgtt ttaatgtta aggcctcgg gtttttctt tgacaaagt gaatgtaaa gaaagcaga aatttcgac actttttcg tttgatttt cgggcacag taaatgtat gatgtacag ggtgtttat tgttcaatc gatgaagag ctcactttt tgattattt ttggaaact tccacttca accaaccct gttgttatt tttgtactt gaactagat ttacacgaa ttgttttta ttgtatata cctacctac caaagttag cacaataga tctcacgct gaaaccgct attgtttca aaactggca tttgtaagc cgaagctta catccatga ccatgtctt tgactgatt tttattcga tttggttgt tgtaggtgc ttgttaaac tcatcataa ttttatttt tatagttag ttcccctat agacacagt gctacgctt atctaagct ataaatgta aaaaagaag tactattta atgaaatta gtaggtaca atgtaggaa aatttactt tccttttct ttattttat ttcggatgc tgcctttgc aggctcatt attgattaa ttgaatgat tattattat tgtataatt gtgtaagca ttatatgaa ataaataaa gaaaaccaa aatat
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