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Gene information
General information
TC006241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5547861..5555737
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006241
    iBeetle screen iB_04098

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva anterior not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    adult nutritional state - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006241 RA
      MLRRFLSLA FLIGAVFTY PNEPKATKN TEKPIITTP 
      LGQVQGAIL ASRAGKPII SFRGIRYAK APIDELRFK 
      PPVPVPKWE NVVNATQDT PLCPQPTDE PTSEDCLFL 
      NVYTTKLPK GNEQPKRPV IVFIHSGGF HSTGGASNW 
      LGPQYLLDQ DIVLVTFNY RLGSLGFLS TGDKEAPGN 
      NGLKDQVLV LKWVKENIA SFGGDPESV TLAGYSAGA 
      ISVTLHLVS PLSRGLFHR AIIMSGSAL GQMPIPTNQ 
      MNLAKKQAK VLECSDDNS ANIIKCLKT KTANQLGES 
      QRMFLEFGD DPVVIWGPV IEGDFGQER FLTAHPIEL 
      IQKGEFAKV PIILGVTTD EFAARAFNV LANETLKKQ 
      MSEDFQKIA PIAFLYDRD SDKSKTISD GLKKFYLND 
      KPLDNSSLP GLAQLYADA LTGFGVNRA AELLSEKNN 
      ASVYYYKFN YKGRYSHFY LPSSNGTIP FGVVHNDDL 
      IYLFYIQKL FPIFKDTDP EAQTVNKMI LLWSNFAKT 
      GNPTPETSD KLDGAKWEP YSSKNKKYL EVGNKLVLG 
      EKLNEKRYE EWAKLFPLS QYTS
    CDS sequence
    • >TC006241 RA
      atgttgagg cggttttta agtttagcg tttctaatt 
      ggcgcggtt tttacttac ccgaatgag cccaaagca 
      actaaaaat accgaaaaa cccattatt actacgcca 
      ctggggcaa gtgcaaggg gccattttg gcctcaagg 
      gcggggaaa cccattatc tctttcagg gggattaga 
      tatgccaaa gctccaatt gacgagttg aggtttaag 
      ccccctgta cctgtcccc aaatgggaa aacgtggtc 
      aacgccacc caagacacc cccttgtgc ccccaaccg 
      actgacgaa cccacttca gaagactgt cttttcctc 
      aacgtttac accacaaaa ctgcctaag ggcaacgag 
      cagcccaaa cgccccgtc attgtcttc atccattct 
      gggggcttc catagcact gggggagcc agcaattgg 
      ctgggcccc caatacctc ctggaccaa gacatcgtc 
      cttgtaact ttcaactat cgactcgga tcgttgggt 
      ttcctaagc actggggac aaggaagct cctggaaac 
      aacggtctt aaagaccaa gttttggtc ctcaagtgg 
      gtcaaagag aatattgcg tcgtttgga ggtgatcca 
      gaatcggtg actttggcg gggtacagc gcaggggct 
      atcagtgtg acccttcac ttggtctca cccctgtca 
      agggggcta ttccatagg gctataatc atgagtggg 
      tcggcgcta ggccaaatg ccaatcccc acaaatcaa 
      atgaatttg gccaagaaa caagccaaa gttttggaa 
      tgtagtgat gataactca gccaatatt atcaaatgt 
      ttgaagacc aaaactgca aatcagttg ggggagtcc 
      cagaggatg tttctggag tttggggat gatccagtt 
      gtgatttgg gggcctgtg attgaaggg gacttcgga 
      caggagagg tttttgacc gctcatcca attgagttg 
      atacaaaag ggagaattt gcaaaagtt ccgattatt 
      ttgggggtt acaactgat gagtttgcc gcaagagct 
      ttcaatgtt ttggccaac gaaacgttg aagaagcag 
      atgagtgaa gacttccaa aaaatcgcc cctattgcc 
      ttcctctac gaccgagat tcggacaaa tcgaaaaca 
      atcagtgat ggcctcaag aaattttat ttgaacgat 
      aaaccttta gacaactcg tctttgccc ggcttggcc 
      caactttac gccgacgct ttgaccggc tttggagtc 
      aacagagcc gcagaactt ttatcagaa aaaaacaac 
      gcctcggtt tattactac aaatttaat tacaaggga 
      cgctacagc cacttttac ctaccctca agtaacgga 
      acaatcccg ttcggtgtt gtgcacaac gacgattta 
      atttacctg ttctacatc cagaaactg ttcccgatt 
      ttcaaagac acggacccc gaagcccaa actgtgaac 
      aagatgatt ttgctttgg tccaatttt gccaaaact 
      ggaaatccg actcctgag accagtgat aagctggat 
      ggggccaaa tgggagcct tacagttcc aaaaataag 
      aagtatttg gaggttggc aacaagttg gttctcggg 
      gaaaaactg aacgagaag agatacgag gagtgggcc 
      aaattgttt cctctgagc caatacaca tcgtaa
    mRNA sequence
    • >TC006241 RA
      tcccttttg tgtcccatc tctataaaa gcgagcgac 
      tgtagcaaa tattttagt ttcgccact agtcgtgct 
      atgtttgat tgtgcaaaa tgttgaggc ggtttttaa 
      gtttagcgt ttctaattg gcgcggttt ttacttacc 
      cgaatgagc ccaaagcaa ctaaaaata ccgaaaaac 
      ccattatta ctacgccac tggggcaag tgcaagggg 
      ccattttgg cctcaaggg cggggaaac ccattatct 
      ctttcaggg ggattagat atgccaaag ctccaattg 
      acgagttga ggtttaagc cccctgtac ctgtcccca 
      aatgggaaa acgtggtca acgccaccc aagacaccc 
      ccttgtgcc cccaaccga ctgacgaac ccacttcag 
      aagactgtc ttttcctca acgtttaca ccacaaaac 
      tgcctaagg gcaacgagc agcccaaac gccccgtca 
      ttgtcttca tccattctg ggggcttcc atagcactg 
      ggggagcca gcaattggc tgggccccc aatacctcc 
      tggaccaag acatcgtcc ttgtaactt tcaactatc 
      gactcggat cgttgggtt tcctaagca ctggggaca 
      aggaagctc ctggaaaca acggtctta aagaccaag 
      ttttggtcc tcaagtggg tcaaagaga atattgcgt 
      cgtttggag gtgatccag aatcggtga ctttggcgg 
      ggtacagcg caggggcta tcagtgtga cccttcact 
      tggtctcac ccctgtcaa gggggctat tccataggg 
      ctataatca tgagtgggt cggcgctag gccaaatgc 
      caatcccca caaatcaaa tgaatttgg ccaagaaac 
      aagccaaag ttttggaat gtagtgatg ataactcag 
      ccaatatta tcaaatgtt tgaagacca aaactgcaa 
      atcagttgg gggagtccc agaggatgt ttctggagt 
      ttggggatg atccagttg tgatttggg ggcctgtga 
      ttgaagggg acttcggac aggagaggt ttttgaccg 
      ctcatccaa ttgagttga tacaaaagg gagaatttg 
      caaaagttc cgattattt tgggggtta caactgatg 
      agtttgccg caagagctt tcaatgttt tggccaacg 
      aaacgttga agaagcaga tgagtgaag acttccaaa 
      aaatcgccc ctattgcct tcctctacg accgagatt 
      cggacaaat cgaaaacaa tcagtgatg gcctcaaga 
      aattttatt tgaacgata aacctttag acaactcgt 
      ctttgcccg gcttggccc aactttacg ccgacgctt 
      tgaccggct ttggagtca acagagccg cagaacttt 
      tatcagaaa aaaacaacg cctcggttt attactaca 
      aatttaatt acaagggac gctacagcc acttttacc 
      taccctcaa gtaacggaa caatcccgt tcggtgttg 
      tgcacaacg acgatttaa tttacctgt tctacatcc 
      agaaactgt tcccgattt tcaaagaca cggaccccg 
      aagcccaaa ctgtgaaca agatgattt tgctttggt 
      ccaattttg ccaaaactg gaaatccga ctcctgaga 
      ccagtgata agctggatg gggccaaat gggagcctt 
      acagttcca aaaataaga agtatttgg aggttggca 
      acaagttgg ttctcgggg aaaaactga acgagaaga 
      gatacgagg agtgggcca aattgtttc ctctgagcc 
      aatacacat cgtaacttt ttacatact tacttgtat 
      tttactgaa agtctgcgt aaataaagg tttttgtac 
      tgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY