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Gene information
General information
TC006235 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 5223236..5225437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006235
    iBeetle screen iB_04097
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006235 RA
      MMSDESEND AVYTESYEN DIYYKKYKL 
      LLEECAQLQ KSNEILVFR IQEVNKIVS 
      RRQKEVNFF RKRLLNNYG EDWTVIPNL 
      DLIKEEEDS KSVAFLMLK DKLKEEKCE 
      EVEKPKPAK KPTKRKSTR SEKDPKAPK 
      RPSNPFFQF CQEQRQILM EQLNAELKP 
      GEAELTKQE LTRQLAIKW KSLSGNDKK 
      VYVDMYERS KEKYAAEMS EYNMKK
    CDS sequence
    • >TC006235 RA
      atgatgtca gatgagtct gaaaacgac gcagtttat 
      accgaaagc tacgaaaat gatatctat tacaaaaaa 
      tacaaactt ttattggaa gaatgtgcc cagttacaa 
      aaatccaac gaaattcta gtttttagg atccaagaa 
      gtgaacaaa atcgtgagt cgacgtcag aaagaggtg 
      aattttttc cgaaaacgc ctcttgaac aattatggc 
      gaagactgg actgtgata cctaaccta gacctcatc 
      aaggaagaa gaagactca aagtcagtt gcgtttctt 
      atgctgaaa gacaaactg aaggaagaa aagtgtgaa 
      gaggtggag aagcccaaa ccagcgaag aaaccaacg 
      aaacggaaa agtacaaga agcgaaaaa gacccaaaa 
      gcccccaag agaccatca aaccccttt ttccagttc 
      tgccaagaa cagagacag attttgatg gaacagtta 
      aatgctgag ttaaagcca ggcgaagct gagctaacc 
      aaacaggaa ttaactaga caattggcg attaaatgg 
      aagtcctta tcagggaat gataagaag gtttacgtg 
      gacatgtac gagaggtcc aaggagaaa tatgcagca 
      gagatgtcg gaatacaac atgaagaag taa
    mRNA sequence
    • >TC006235 RA
      agcttaata aggtggtgg gtacaatag ctttgtctc 
      tctcacgag atgcgattg ggtgtcttt gtttgacac 
      aatgagtga gacagagag gggattgcc gtttcacgg 
      tcagatgtt tatcgattt taaatagag agctatttt 
      tgaacgaca tggtacgac ttgaattga ggaaatgac 
      aaaatttaa aaaaattgc aaccaacct taaaaaaat 
      caaaatgca acaagctac taaagcaca caataacct 
      actggaggg aataaaatc ttccaacta ttgtaattc 
      cacatgtgt cgtcaagat gtttcaatc tcggaaaaa 
      tttcgattt tatttaaca tttttcgaa cgtaaacct 
      catttcgat aattttata aacggaaat ttattttta 
      accaagctc tcaattcat tcatccagt tttgaggat 
      cagtcaaca caaatcgag catttaatg aaaaactaa 
      tttaatcaa aatactttt aaatctagt cacactgct 
      gtgacgcaa ataaattga ctcaatttt gacgctatt 
      tttatcact ctgtgaatt ccattgcaa ttaccacga 
      tttttctat atttctttt tcaaaattt cctaatttt 
      tgtaagagt tgtcaaata ggcggagtc acttttgaa 
      gaaggcggg gcaaaacag tacgtgggc ggggtaaac 
      attttagag aaactaata attttaaac taaagaaaa 
      atacatttt ttcgcgcct attggattt cgtccataa 
      ttttgacgc atttgtctt ttttgaggt tagtttttt 
      taacctcta aatttttaa ataatcttc gactaataa 
      ataatttaa tcagagatg gattttggc gatttatct 
      gaggataaa gcaatccaa accaaactt tccaatttt 
      cattcaagt gttgaaaaa atcgaaaaa attatagaa 
      atatcctca agtcccgat atttacgac aaactgacg 
      actaaggaa aaagtcgat tatgattta ttcatggct 
      tatacactg aacacttta ttttggcta tacttaaag 
      actaaaggt gaagacccc actaaaagt gaaatcaaa 
      aatcagtta aatcgagtc aaacaatac atggtgaaa 
      gccaaagag gcccatgag agacaagtc ttgagacct 
      cgaatagac tgtggggcc gcagggcga ttcattaaa 
      cacggaata aattataaa gacagtggg acacccgag 
      gagccccct aataaaaaa atgaagttt tctgatgat 
      taatttagc gttttcttg taaaataaa taaaaaagt 
      ttcgtaatt taagtcatt ttattttct gaaagtgac 
      agttgtatg taaacttaa cctacaaaa ctttggttc 
      tgtctcgaa aaatgatgt cagatgagt ctgaaaacg 
      acgcagttt ataccgaaa gctacgaaa atgatatct 
      attacaaaa aatacaaac ttttattgg aagaatgtg 
      cccagttac aaaaatcca acgaaattc tagttttta 
      ggatccaag aagtgaaca aaatcgtga gtcgacgtc 
      agaaagagg tgaattttt tccgaaaac gcctcttga 
      acaattatg gcgaagact ggactgtga tacctaacc 
      tagacctca tcaaggaag aagaagact caaagtcag 
      ttgcgtttc ttatgctga aagacaaac tgaaggaag 
      aaaagtgtg aagaggtgg agaagccca aaccagcga 
      agaaaccaa cgaaacgga aaagtacaa gaagcgaaa 
      aagacccaa aagccccca agagaccat caaacccct 
      ttttccagt tctgccaag aacagagac agattttga 
      tggaacagt taaatgctg agttaaagc caggcgaag 
      ctgagctaa ccaaacagg aattaacta gacaattgg 
      cgattaaat ggaagtcct tatcaggga atgataaga 
      aggtttacg tggacatgt acgagaggt ccaaggaga 
      aatatgcag cagagatgt cggaataca acatgaaga 
      agtaaataa tgtattatt taaataaaa atttattct 
      ttgt