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Gene information
General information
TC006229 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 5152006..5164332
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006229
    iBeetle screen iB_04095
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006229 RA
      MVGVLVGVL LVGVIWGCD CHQKPYSYQ YVRREIERV 
      FAEEEANRW LCFVFGICA IDAAYPLKP DLAIVYQWS 
      QLEFDYPSI EDRQADIDA GNFIPGKPA PIDVDVHYS 
      YSPHKKSRV FVAIPRFQD GVPIALGVV TDKKFNSSN 
      VITPYPSWD WHKTPSKCN ENRIVSVYR VSIDECQRL 
      WVLDTGRLK ETQVCPPQI LAFDLQTDQ LIHRFQLPK 
      GIVEPRSIL VTPVVDVRD AHAKCKDTF IYIADCQTY 
      SIIVYDVQK GVIWKATDK TMYPYPNYG TFDILGDSF 
      DLMDGVLGM SLSPYKPGQ DRILYYHAM SSPTENWVL 
      TSDLRNRSR FIHDSQSSP EIFHTYHGK RHTQSAAEA 
      IDKDGIMYF GLMSDVTLN CWNTHTEYG PKTIDIIAK 
      NLQTLQFAS GVKVITNSK AGQELWVLT SRFQKVATD 
      SLSTSDVNF RILAGRTED MVAGTACRG GIPPGPPGG 
      GYGLVKPPF VPEAHHKKP HLGF
    CDS sequence
    • >TC006229 RA
      atggtagga gtgctggtc ggggttttg ctagtgggt 
      gtgatatgg ggatgtgac tgccatcag aagccttac 
      tcttaccag tacgttcgc agagagatc gagcgcgtc 
      tttgccgaa gaggaagcc aatcgctgg ttgtgcttc 
      gttttcggc atatgtgcc atcgacgcc gcctatccc 
      ctgaagccc gacttggcc attgtgtac caatggtcc 
      cagctcgaa ttcgattat ccgtccatt gaggatcgt 
      caagctgac atcgatgcg ggaaatttc atcccggga 
      aaaccggcg cccatcgac gtggacgtc cattattca 
      tatagccca cataagaaa agccgcgtt tttgtggca 
      atcccgcgc ttccaggat ggggtacct atagcgctg 
      ggtgtcgta actgataag aaatttaac agtagtaat 
      gttatcacg ccgtaccca tcatgggac tggcataaa 
      acgccatca aagtgcaat gagaatagg attgtgtcg 
      gtttaccgg gtttcgatc gacgaatgc cagagatta 
      tgggtcttg gacaccggc cgtctaaag gagacccaa 
      gtgtgccca cctcaaatt ctggccttt gatctccaa 
      acagaccaa ctgatccac agatttcag cttccaaaa 
      ggaattgtt gaaccaaga tcaattttg gtgactcct 
      gtagttgac gttagggac gctcatgct aagtgtaaa 
      gacactttt atctacatt gccgattgc cagacttat 
      tccatcata gtttatgac gtccaaaag ggcgttatt 
      tggaaggcc acagacaaa accatgtac ccatacccc 
      aattatggc accttcgac attttaggt gatagtttt 
      gacttgatg gacggcgtc ctgggcatg tccctatcg 
      ccctataaa ccaggccag gaccgtatt ttgtactac 
      catgccatg tcaagcccc accgaaaat tgggtcttg 
      acctccgac ctccgcaac cgctccaga ttcatccac 
      gattcgcaa tcatcgccg gaaatcttc catacatac 
      cacggcaaa agacacacc cagtccgct gcagaagcc 
      atcgacaaa gacggcatc atgtacttc ggtctaatg 
      agcgacgtc acactcaac tgctggaac actcacact 
      gaatacggc ccgaaaacc attgacatt atcgcgaaa 
      aacttgcaa actctgcag tttgccagt ggtgttaaa 
      gtgattacc aatagcaag gccgggcaa gagttgtgg 
      gttttgacg tcgcggttc cagaaagtt gccacggat 
      tcgttatca acgtccgat gttaatttc aggattctg 
      gcagggagg actgaagac atggtcgct gggacggcg 
      tgcaggggc ggaatacca ccaggacca cctgggggt 
      ggctatggc ttggtgaag ccgccattc gtgcccgaa 
      gctcaccac aagaagccc catttgggt ttttaa
    mRNA sequence
    • >TC006229 RA
      ttttagaga attgggcca aaattcgga aacagtttt 
      ttcttcgga caacctttg accgttgaa aaacctcgc 
      caaaaccag tcccaaatc ttcgagttg gtggaagcg 
      cctcataac tcatctgcc tatatatat ataattttt 
      cacatgtgt tccaccaac tgtcagtgt ttcagtgtt 
      cgtcgtgcg taaatccaa ctttgagat gacaagagg 
      ctattcaaa gctcatcaa catctgtga ttggaatgg 
      acaggggaa tgttatata agaattgaa agtgtcaga 
      aatttgatc aaatcggtt cagtttgct ggctgacca 
      tgatggtgg tgagacgat ggtaggagt gctggtcgg 
      ggttttgct agtgggtgt gatatgggg atgtgactg 
      ccatcagaa gccttactc ttaccagta cgttcgcag 
      agagatcga gcgcgtctt tgccgaaga ggaagccaa 
      tcgctggtt gtgcttcgt tttcggcat atgtgccat 
      cgacgccgc ctatcccct gaagcccga cttggccat 
      tgtgtacca atggtccca gctcgaatt cgattatcc 
      gtccattga ggatcgtca agctgacat cgatgcggg 
      aaatttcat cccgggaaa accggcgcc catcgacgt 
      ggacgtcca ttattcata tagcccaca taagaaaag 
      ccgcgtttt tgtggcaat cccgcgctt ccaggatgg 
      ggtacctat agcgctggg tgtcgtaac tgataagaa 
      atttaacag tagtaatgt tatcacgcc gtacccatc 
      atgggactg gcataaaac gccatcaaa gtgcaatga 
      gaataggat tgtgtcggt ttaccgggt ttcgatcga 
      cgaatgcca gagattatg ggtcttgga caccggccg 
      tctaaagga gacccaagt gtgcccacc tcaaattct 
      ggcctttga tctccaaac agaccaact gatccacag 
      atttcagct tccaaaagg aattgttga accaagatc 
      aattttggt gactcctgt agttgacgt tagggacgc 
      tcatgctaa gtgtaaaga cacttttat ctacattgc 
      cgattgcca gacttattc catcatagt ttatgacgt 
      ccaaaaggg cgttatttg gaaggccac agacaaaac 
      catgtaccc ataccccaa ttatggcac cttcgacat 
      tttaggtga tagttttga cttgatgga cggcgtcct 
      gggcatgtc cctatcgcc ctataaacc aggccagga 
      ccgtatttt gtactacca tgccatgtc aagccccac 
      cgaaaattg ggtcttgac ctccgacct ccgcaaccg 
      ctccagatt catccacga ttcgcaatc atcgccgga 
      aatcttcca tacatacca cggcaaaag acacaccca 
      gtccgctgc agaagccat cgacaaaga cggcatcat 
      gtacttcgg tctaatgag cgacgtcac actcaactg 
      ctggaacac tcacactga atacggccc gaaaaccat 
      tgacattat cgcgaaaaa cttgcaaac tctgcagtt 
      tgccagtgg tgttaaagt gattaccaa tagcaaggc 
      cgggcaaga gttgtgggt tttgacgtc gcggttcca 
      gaaagttgc cacggattc gttatcaac gtccgatgt 
      taatttcag gattctggc agggaggac tgaagacat 
      ggtcgctgg gacggcgtg caggggcgg aataccacc 
      aggaccacc tgggggtgg ctatggctt ggtgaagcc 
      gccattcgt gcccgaagc tcaccacaa gaagcccca 
      tttgggttt ttaaagctt tattattta ttgtattag 
      ttagagatg tttgaactt tgaatagat tgtttgcag 
      ttatgaaat aaataatac caagtttt