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Gene information
General information
TC006227 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 5130326..5140305
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006227
    iBeetle screen iB_04094
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 55.56% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006227 RA
      MLRHALFLL FLSPVLSLE LEVVNQWNL LDFQFPFDH 
      RLINNFRPE RNVFTGLEI TNDRIFLAV PRLWSGVSA 
      TLAVIPRHT PPGSSPMLQ AYPNWEAHK FGRGQNDSC 
      DGLTSVYRI RADSCNRLW VLDSGVNLA LEDFQRVCP 
      PKLVVFDLE TDQIVKTVV FPRQVLRPN SLLTNLVID 
      ETGHGGCER AFVYMSDTA APGLVVYDS RHDTAWRLM 
      HPTMFPDPD FSDYTVAGE RFTLMDGVV GLTHSPKLG 
      ILYFQPLAT DRLFSIPTS ALQKGPPGQ DEELPITLV 
      GKKSSQGLG LALDPKDDT IFFSPVRET SIAAWNPVT 
      NHQKLIAYD PQALQFAAE LRWKDEDQS IWLLSTRFQ 
      KFFRRTISP HEVNLRIIR ISTAPRVAS GVTNHLFY
    CDS sequence
    • >TC006227 RA
      atgttacgc cacgcactc ttccttcta ttcttatca 
      cctgtccta tcactggaa ctcgaggtg gtcaaccag 
      tggaatttg ctcgacttt caattcccc ttcgaccac 
      cgcctcatc aacaacttc cgcccggaa cggaacgtt 
      ttcaccggt ttggaaatc accaatgac cggattttc 
      ctcgcagtt ccgcgcctc tggtcgggc gtttccgcc 
      acgttggcg gtgattccc cgacacacg ccgcctgga 
      tcgagtcca atgctgcaa gcgtacccc aattgggag 
      gcccataag ttcgggagg ggtcaaaat gacagctgt 
      gacgggctt acttcagtc tacagaatc agggcggat 
      tcatgcaac cgactttgg gttctagat tcgggggtt 
      aacctagcc cttgaggac ttccaacgt gtgtgtcct 
      ccaaaattg gtcgttttt gatctagag accgaccaa 
      attgtgaaa actgttgtt ttcccaaga caagttctg 
      cgccccaat tcgctgcta actaactta gttattgat 
      gagacggga catgggggc tgcgagcgg gcttttgtc 
      tacatgtct gacacagca gcaccaggt ttggttgtg 
      tatgattcg aggcatgac acagcctgg cgtcttatg 
      caccccacg atgttccca gaccccgac ttttccgat 
      tatactgtc gcaggggag cgtttcacc cttatggat 
      ggggtggtg gggctcaca cactcgccc aagttagga 
      attttgtat ttccagccc ctggctact gaccgtttg 
      tttagtatc ccgacgtca gccctacag aagggcccc 
      ccaggccaa gacgaagaa ctccccatt actctagtc 
      gggaaaaaa tcatcgcaa ggtttaggt ctagccctt 
      gacccaaaa gacgacacg attttcttc agtccggtt 
      agagagacg tcaattgcg gcctggaat ccggttacc 
      aaccaccaa aagttaatc gcctatgac ccgcaagcg 
      cttcaattc gctgctgaa ttgcgatgg aaggatgag 
      gaccaatcg atttggttg ttgtctaca aggtttcag 
      aagtttttc aggaggact attagtccc cacgaggtc 
      aatttgagg ataattagg atcagtacg gctccaaga 
      gttgcttcc ggagtaacc aatcatttg ttttattaa
    mRNA sequence
    • >TC006227 RA
      gggaaataa tacgcttct ggcgggtgt gtctacgat 
      ttgggggct cgccgaggc catcactca aagggggga 
      ccggcgaaa gtaccccag aacttaaga tctgccggc 
      actttatta ccaaatagt ggcagtgct cgaacagtg 
      cgtgtaaca agtgaggat gttacgcca cgcactctt 
      ccttctatt cttatcacc tgtcctatc actggaact 
      cgaggtggt caaccagtg gaatttgct cgactttca 
      attcccctt cgaccaccg cctcatcaa caacttccg 
      cccggaacg gaacgtttt caccggttt ggaaatcac 
      caatgaccg gattttcct cgcagttcc gcgcctctg 
      gtcgggcgt ttccgccac gttggcggt gattccccg 
      acacacgcc gcctggatc gagtccaat gctgcaagc 
      gtaccccaa ttgggaggc ccataagtt cgggagggg 
      tcaaaatga cagctgtga cgggcttac ttcagtcta 
      cagaatcag ggcggattc atgcaaccg actttgggt 
      tctagattc gggggttaa cctagccct tgaggactt 
      ccaacgtgt gtgtcctcc aaaattggt cgtttttga 
      tctagagac cgaccaaat tgtgaaaac tgttgtttt 
      cccaagaca agttctgcg ccccaattc gctgctaac 
      taacttagt tattgatga gacgggaca tgggggctg 
      cgagcgggc ttttgtcta catgtctga cacagcagc 
      accaggttt ggttgtgta tgattcgag gcatgacac 
      agcctggcg tcttatgca ccccacgat gttcccaga 
      ccccgactt ttccgatta tactgtcgc aggggagcg 
      tttcaccct tatggatgg ggtggtggg gctcacaca 
      ctcgcccaa gttaggaat tttgtattt ccagcccct 
      ggctactga ccgtttgtt tagtatccc gacgtcagc 
      cctacagaa gggcccccc aggccaaga cgaagaact 
      ccccattac tctagtcgg gaaaaaatc atcgcaagg 
      tttaggtct agcccttga cccaaaaga cgacacgat 
      tttcttcag tccggttag agagacgtc aattgcggc 
      ctggaatcc ggttaccaa ccaccaaaa gttaatcgc 
      ctatgaccc gcaagcgct tcaattcgc tgctgaatt 
      gcgatggaa ggatgagga ccaatcgat ttggttgtt 
      gtctacaag gtttcagaa gtttttcag gaggactat 
      tagtcccca cgaggtcaa tttgaggat aattaggat 
      cagtacggc tccaagagt tgcttccgg agtaaccaa 
      tcatttgtt ttattaatc gacggaatt accaatttg 
      ttaagtgta aaaatgtat ttatttttg tagagattg 
      tgttttatg tattttgac tggtcatca agtgtattg 
      taaataagt gaatcaata aatttgagg aagtgac