Status
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Gene information
General information
TC033998
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006221
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5314826..5327580
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033998
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04091

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033998 RA
      MDSLTLLWY DYLIFILVL LFSISIGIY FGFFAKNNQ 
      SDPSEYLQG KKQMSPLPV AVSLTASST SVSILLSYS 
      SEAYSFGAN TFLLIFGMI IAYCANKFI YLPVFYSLE 
      IDTVYEYLE KRFDKRARQ LASSIFTIC CVCFMSMTI 
      YTPALALSA VTGFKLQYI IVGVSCVCI FYTSVGGFK 
      TVIWTDVLQ FFVVLASLL TTLGLAVGS SGGFRQMWH 
      KAVTSGRLD IFEFDPNPT RHSSVWIIV IGYSFTFMS 
      ITCVDQIFV QKTRSIRNI ESASKALIF FTVGHYFIV 
      VVCILLGLA MYAKYWNCD PVSANFIQK PDQLLPYLV 
      MDVGNQIPG VSGIFVSAI FSSSLSLQS AILNCLSGS 
      IYSDFLKPF VGEIPESRI LKLTVLGLG VLCTVLAFV 
      IQYLGTLFH IIFTIVGAT GGPILGMFT CGLLVPRIN 
      SKATFYGTL SGFVICLSI ALPATYYQA QSGQNQYLK 
      PISTSCLNQ TLRNETTTD QSKSPWLFQ ISYFYYSVI 
      GTIVTAIVA FFVSCMTQK KNFHVDKNL ISPIARFLV 
      EKAPENSGR SPTLNGYLS IGDAFSGGV ATWAPAPRG 
      RRDVGSNVG GDMRVEKET FTTEKYVQW V
    • >TC033998 RB
      MDSLTLLWY DYLIFILVL LFSISIGIY FGFFAKNNQ 
      SDPSEYLQG KKQMSPLPV AVSLTASST SVSILLSYS 
      SEAYSFGAN TFLLIFGMI IAYCANKFI YLPVFYSLE 
      IDTVYEYLE KRFDKRARQ LASSIFTIC CVCFMSMTI 
      YTPALALSA VTGFKLQYI IVGVSCVCI FYTSVGGFK 
      TVIWTDVLQ FFVVLASLL TTLGLAVGS SGGFRQMWH 
      KAVTSGRLD IFEFDPNPT RHSSVWIIV IGYSFTFMS 
      ITCVDQIFV QKTRSIRNI ESASKALIF FTVGHYFIV 
      VVCILLGLA MYAKYWNCD PVSANFIQK PDQLLPYLV 
      MDVGNQIPG VSGIFVSAI FSSSLSLQS AILNCLSGS 
      IYSDFLKPF VGEIPESRI LKLTVLGLG VLCTVLAFV 
      IQYLGTLFH IIFTIVGAT GGPILGMFT CGLLVPRIN 
      SKGAFYGTL SGFVICLSI ALPATYYQA QSGQNQYLK 
      PISTSCLNQ TLRNETTTD QSKSPWLFQ ISYFYYSVI 
      GTIVTAIVA FFVSCMTQK KNFHVDKNL ISPIARFLV 
      EKAPENSGR SPTLNGYLS IGDAFSGGV ATWAPAPRG 
      RRDVGSNVG GDMRVEKET FTTEKYVQW V
    CDS sequence
    • >TC033998 RA
      atggactct ctcacacta ctatggtac gactactta 
      atcttcatt ttggtccta ctttttagc atttcaatc 
      ggtatttat ttcggtttc ttcgccaaa aacaaccaa 
      tcagaccct tcggagtac ctccaaggc aagaaacag 
      atgagccct ctcccagtt gccgtctca ctaaccgca 
      agttccacc tccgtttcc attcttctg agctacagt 
      tcagaagcg tacagtttt ggcgccaac acgtttttg 
      ctcattttt ggcatgatt atagcctac tgtgccaac 
      aaattcatc tatttgccc gtgttctac agtcttgaa 
      attgacaca gtttatgaa tatttggag aaacggttt 
      gataaaagg gcaagacaa ctcgcatca agcattttc 
      acgatttgt tgcgtctgt ttcatgtcc atgaccatt 
      tatacgccg gctttggcc ctatcagct gtcaccgga 
      tttaaacta caatacatt atagtgggg gtcagctgt 
      gtttgcatt ttttacaca agtgttggc ggctttaag 
      accgttatt tggaccgat gtgctgcaa tttttcgtg 
      gttttggct tctttgctc actactttg ggcctagca 
      gttgggtca agtggcggt tttaggcaa atgtggcac 
      aaagcggtg acaagtggc cgtttggat attttcgaa 
      tttgatcca aatccgacg cgacatagc agtgtttgg 
      attatagta atcggatat tcgttcaca tttatgtca 
      atcacttgt gttgatcag atttttgtc cagaaaact 
      cgctcgatt cgcaacatt gaaagcgcg tcaaaagcc 
      ttgattttt ttcacagtt ggtcactat tttattgtt 
      gttgtttgt attttgttg ggcttggct atgtatgca 
      aaatattgg aattgtgac ccagtcagt gccaatttt 
      atccaaaaa ccggaccag ttactgcct tatcttgtt 
      atggatgtg gggaatcaa atccccgga gtttccggg 
      atttttgtt tcagcaatt ttcagctcg agtttgagt 
      cttcagtca gcaattttg aactgtttg tctggttca 
      atatactcg gatttttta aaaccattt gtaggggaa 
      atccccgaa agcagaatt ttgaaattg acagtcctg 
      ggacttggt gttttatgc acagttttg gcttttgta 
      atacaatat ttagggact ttattccac attattttt 
      acgatagta ggggcgaca ggaggccct attttaggg 
      atgttcact tgcgggctt ttggtacca agaattaac 
      tccaaggca acattttat ggtactctc tctggattt 
      gtgatttgt ctgagtatt gctttacca gcaacttat 
      taccaggcc caaagtgga caaaaccaa tatttgaaa 
      cccatttcg accagttgt ttgaatcaa actttgcga 
      aatgaaact acaactgac caatcgaag tctccctgg 
      ttgtttcaa atttcatac ttttactat tcagtcatt 
      ggtacaatt gtcacagca attgtggca tttttcgtt 
      agttgtatg actcaaaaa aagaatttt catgtcgac 
      aaaaattta attagtcca atcgctcgt tttctcgtg 
      gaaaaagct cccgaaaac tccgggcgt tcgcccact 
      ctcaatgga tatttatca ataggcgac gctttcagt 
      ggcggcgtc gcgacgtgg gcgcccgca ccacgtgga 
      cggcgtgac gtcggctcc aacgtggga ggagacatg 
      cgcgtggag aaggagacg tttacaacc gagaaatat 
      gttcagtgg gtttga
    • >TC033998 RB
      atggactct ctcacacta ctatggtac gactactta 
      atcttcatt ttggtccta ctttttagc atttcaatc 
      ggtatttat ttcggtttc ttcgccaaa aacaaccaa 
      tcagaccct tcggagtac ctccaaggc aagaaacag 
      atgagccct ctcccagtt gccgtctca ctaaccgca 
      agttccacc tccgtttcc attcttctg agctacagt 
      tcagaagcg tacagtttt ggcgccaac acgtttttg 
      ctcattttt ggcatgatt atagcctac tgtgccaac 
      aaattcatc tatttgccc gtgttctac agtcttgaa 
      attgacaca gtttatgaa tatttggag aaacggttt 
      gataaaagg gcaagacaa ctcgcatca agcattttc 
      acgatttgt tgcgtctgt ttcatgtcc atgaccatt 
      tatacgccg gctttggcc ctatcagct gtcaccgga 
      tttaaacta caatacatt atagtgggg gtcagctgt 
      gtttgcatt ttttacaca agtgttggc ggctttaag 
      accgttatt tggaccgat gtgctgcaa tttttcgtg 
      gttttggct tctttgctc actactttg ggcctagca 
      gttgggtca agtggcggt tttaggcaa atgtggcac 
      aaagcggtg acaagtggc cgtttggat attttcgaa 
      tttgatcca aatccgacg cgacatagc agtgtttgg 
      attatagta atcggatat tcgttcaca tttatgtca 
      atcacttgt gttgatcag atttttgtc cagaaaact 
      cgctcgatt cgcaacatt gaaagcgcg tcaaaagcc 
      ttgattttt ttcacagtt ggtcactat tttattgtt 
      gttgtttgt attttgttg ggcttggct atgtatgca 
      aaatattgg aattgtgac ccagtcagt gccaatttt 
      atccaaaaa ccggaccag ttactgcct tatcttgtt 
      atggatgtg gggaatcaa atccccgga gtttccggg 
      atttttgtt tcagcaatt ttcagctcg agtttgagt 
      cttcagtca gcaattttg aactgtttg tctggttca 
      atatactcg gatttttta aaaccattt gtaggggaa 
      atccccgaa agcagaatt ttgaaattg acagtcctg 
      ggacttggt gttttatgc acagttttg gcttttgta 
      atacaatat ttagggact ttattccac attattttt 
      acgatagta ggggcgaca ggaggccct attttaggg 
      atgttcact tgcgggctt ttggtacca agaattaac 
      tccaaggga gcattttat ggtactctc tctggattt 
      gtgatttgt ctgagtatt gctttacca gcaacttat 
      taccaggcc caaagtgga caaaaccaa tatttgaaa 
      cccatttcg accagttgt ttgaatcaa actttgcga 
      aatgaaact acaactgac caatcgaag tctccctgg 
      ttgtttcaa atttcatac ttttactat tcagtcatt 
      ggtacaatt gtcacagca attgtggca tttttcgtt 
      agttgtatg actcaaaaa aagaatttt catgtcgac 
      aaaaattta attagtcca atcgctcgt tttctcgtg 
      gaaaaagct cccgaaaac tccgggcgt tcgcccact 
      ctcaatgga tatttatca ataggcgac gctttcagt 
      ggcggcgtc gcgacgtgg gcgcccgca ccacgtgga 
      cggcgtgac gtcggctcc aacgtggga ggagacatg 
      cgcgtggag aaggagacg tttacaacc gagaaatat 
      gttcagtgg gtttga
    mRNA sequence
    • >TC033998 RA
      ttacaaaac attatcaaa tttttgacc gcaatggac 
      tctctcaca ctactatgg tacgactac ttaatcttc 
      attttggtc ctacttttt agcatttca atcggtatt 
      tatttcggt ttcttcgcc aaaaacaac caatcagac 
      ccttcggag tacctccaa ggcaagaaa cagatgagc 
      cctctccca gttgccgtc tcactaacc gcaagttcc 
      acctccgtt tccattctt ctgagctac agttcagaa 
      gcgtacagt tttggcgcc aacacgttt ttgctcatt 
      tttggcatg attatagcc tactgtgcc aacaaattc 
      atctatttg cccgtgttc tacagtctt gaaattgac 
      acagtttat gaatatttg gagaaacgg tttgataaa 
      agggcaaga caactcgca tcaagcatt ttcacgatt 
      tgttgcgtc tgtttcatg tccatgacc atttatacg 
      ccggctttg gccctatca gctgtcacc ggatttaaa 
      ctacaatac attatagtg ggggtcagc tgtgtttgc 
      attttttac acaagtgtt ggcggcttt aagaccgtt 
      atttggacc gatgtgctg caatttttc gtggttttg 
      gcttctttg ctcactact ttgggccta gcagttggg 
      tcaagtggc ggttttagg caaatgtgg cacaaagcg 
      gtgacaagt ggccgtttg gatattttc gaatttgat 
      ccaaatccg acgcgacat agcagtgtt tggattata 
      gtaatcgga tattcgttc acatttatg tcaatcact 
      tgtgttgat cagattttt gtccagaaa actcgctcg 
      attcgcaac attgaaagc gcgtcaaaa gccttgatt 
      tttttcaca gttggtcac tattttatt gttgttgtt 
      tgtattttg ttgggcttg gctatgtat gcaaaatat 
      tggaattgt gacccagtc agtgccaat tttatccaa 
      aaaccggac cagttactg ccttatctt gttatggat 
      gtggggaat caaatcccc ggagtttcc gggattttt 
      gtttcagca attttcagc tcgagtttg agtcttcag 
      tcagcaatt ttgaactgt ttgtctggt tcaatatac 
      tcggatttt ttaaaacca tttgtaggg gaaatcccc 
      gaaagcaga attttgaaa ttgacagtc ctgggactt 
      ggtgtttta tgcacagtt ttggctttt gtaatacaa 
      tatttaggg actttattc cacattatt tttacgata 
      gtaggggcg acaggaggc cctatttta gggatgttc 
      acttgcggg cttttggta ccaagaatt aactccaag 
      gcaacattt tatggtact ctctctgga tttgtgatt 
      tgtctgagt attgcttta ccagcaact tattaccag 
      gcccaaagt ggacaaaac caatatttg aaacccatt 
      tcgaccagt tgtttgaat caaactttg cgaaatgaa 
      actacaact gaccaatcg aagtctccc tggttgttt 
      caaatttca tacttttac tattcagtc attggtaca 
      attgtcaca gcaattgtg gcatttttc gttagttgt 
      atgactcaa aaaaagaat tttcatgtc gacaaaaat 
      ttaattagt ccaatcgct cgttttctc gtggaaaaa 
      gctcccgaa aactccggg cgttcgccc actctcaat 
      ggatattta tcaataggc gacgctttc agtggcggc 
      gtcgcgacg tgggcgccc gcaccacgt ggacggcgt 
      gacgtcggc tccaacgtg ggaggagac atgcgcgtg 
      gagaaggag acgtttaca accgagaaa tatgttcag 
      tgggtttga tgacggtgt caatgtgtg tgcgcaaaa 
      agtgacact tgtactatc cacacacgt gagaacgta 
      agt
    • >TC033998 RB
      ttacaaaac attatcaaa tttttgacc gcaatggac 
      tctctcaca ctactatgg tacgactac ttaatcttc 
      attttggtc ctacttttt agcatttca atcggtatt 
      tatttcggt ttcttcgcc aaaaacaac caatcagac 
      ccttcggag tacctccaa ggcaagaaa cagatgagc 
      cctctccca gttgccgtc tcactaacc gcaagttcc 
      acctccgtt tccattctt ctgagctac agttcagaa 
      gcgtacagt tttggcgcc aacacgttt ttgctcatt 
      tttggcatg attatagcc tactgtgcc aacaaattc 
      atctatttg cccgtgttc tacagtctt gaaattgac 
      acagtttat gaatatttg gagaaacgg tttgataaa 
      agggcaaga caactcgca tcaagcatt ttcacgatt 
      tgttgcgtc tgtttcatg tccatgacc atttatacg 
      ccggctttg gccctatca gctgtcacc ggatttaaa 
      ctacaatac attatagtg ggggtcagc tgtgtttgc 
      attttttac acaagtgtt ggcggcttt aagaccgtt 
      atttggacc gatgtgctg caatttttc gtggttttg 
      gcttctttg ctcactact ttgggccta gcagttggg 
      tcaagtggc ggttttagg caaatgtgg cacaaagcg 
      gtgacaagt ggccgtttg gatattttc gaatttgat 
      ccaaatccg acgcgacat agcagtgtt tggattata 
      gtaatcgga tattcgttc acatttatg tcaatcact 
      tgtgttgat cagattttt gtccagaaa actcgctcg 
      attcgcaac attgaaagc gcgtcaaaa gccttgatt 
      tttttcaca gttggtcac tattttatt gttgttgtt 
      tgtattttg ttgggcttg gctatgtat gcaaaatat 
      tggaattgt gacccagtc agtgccaat tttatccaa 
      aaaccggac cagttactg ccttatctt gttatggat 
      gtggggaat caaatcccc ggagtttcc gggattttt 
      gtttcagca attttcagc tcgagtttg agtcttcag 
      tcagcaatt ttgaactgt ttgtctggt tcaatatac 
      tcggatttt ttaaaacca tttgtaggg gaaatcccc 
      gaaagcaga attttgaaa ttgacagtc ctgggactt 
      ggtgtttta tgcacagtt ttggctttt gtaatacaa 
      tatttaggg actttattc cacattatt tttacgata 
      gtaggggcg acaggaggc cctatttta gggatgttc 
      acttgcggg cttttggta ccaagaatt aactccaag 
      ggagcattt tatggtact ctctctgga tttgtgatt 
      tgtctgagt attgcttta ccagcaact tattaccag 
      gcccaaagt ggacaaaac caatatttg aaacccatt 
      tcgaccagt tgtttgaat caaactttg cgaaatgaa 
      actacaact gaccaatcg aagtctccc tggttgttt 
      caaatttca tacttttac tattcagtc attggtaca 
      attgtcaca gcaattgtg gcatttttc gttagttgt 
      atgactcaa aaaaagaat tttcatgtc gacaaaaat 
      ttaattagt ccaatcgct cgttttctc gtggaaaaa 
      gctcccgaa aactccggg cgttcgccc actctcaat 
      ggatattta tcaataggc gacgctttc agtggcggc 
      gtcgcgacg tgggcgccc gcaccacgt ggacggcgt 
      gacgtcggc tccaacgtg ggaggagac atgcgcgtg 
      gagaaggag acgtttaca accgagaaa tatgttcag 
      tgggtttga tgacggtgt caatgtgtg tgcgcaaaa 
      agtgacact tgtactatc cacacacgt gagaacgta 
      agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY