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Gene information
General information
TC006218 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5197018..5203045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006218
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04090

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment potentially partiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006218 RA
      MEPVPEFEE LMLCGYCKQ KFNDSDQSP KLLSCKHYF 
      CLQCMRTNL NKGQELYCV YCWKRTEIG DLGPESLPT 
      YAPVLCLTK NFSQMKLGP KPPDKPAKV ISENCHTHA 
      MPLALWCHT CCAPVCRAC ATTSDHSTH QIKSQNEAK 
      EQLVAELQV DLASMNKML SEIQRLAIQ QREFLLKIL 
      EACVTLKTH VEADLTNNA SHFELTETR DTLAKLRLN 
      LSMAENLTE VHNLYSSVS VEKQRLQLR YQEMYLQCQ 
      LDDLIRNST VIFDFQLLK QALSNIHNG EMAFPGNSR 
      ILPPSQQNP ILFLANYCM SQLYSRHVL TKQTVNGSL 
      DYHNHNTIP PVNYHPQHL VLTTPKNIY QENNLMMQS 
      VIHSPQMRN PANMCPLYY FNIEVNGAS VGRIVIEVR 
      ADVAPKMAK NFGLLTTGE AGMGYKGCQ IFQCWEGES 
      VITGDFELN NGRGGRSIF EDSYFLPDD TKLMAVRGT 
      VGMRRTQKR HDNLGMVGS QFRIILQEM RGFTGIFGH 
      VIEGLELVE KISTYGDTA GKPTKNILI AKCGKL
    CDS sequence
    • >TC006218 RA
      atggaaccg gtgcccgaa ttcgaggag ctgatgctg 
      tgcggctac tgcaagcag aaattcaac gactccgac 
      cagagcccc aaactgcta tcgtgcaaa cactacttc 
      tgcctccaa tgcatgcgc accaacctg aacaagggc 
      caggagctg tactgcgtg tactgctgg aagcgcacc 
      gaaatcggc gacctgggg cccgagtcg ctcccgacc 
      tacgccccc gtgctctgc ctcacgaag aacttctcg 
      cagatgaag ctgggcccc aagcccccg gacaaaccc 
      gccaaagtc atatcggag aattgccac acgcacgcc 
      atgcccctg gcgctctgg tgccacacg tgctgcgcc 
      cccgtgtgc cgcgcctgc gccaccacc agcgaccat 
      tccacccac caaatcaaa tcgcagaac gaagccaag 
      gagcagctc gtcgccgag ctgcaggtc gacctcgcg 
      tcgatgaat aaaatgctc tcggagatc cagaggctt 
      gcgatacaa cagcgcgaa ttcttgctg aaaatactc 
      gaagcctgc gttacgcta aagacgcac gtcgaggcc 
      gatttgacc aacaatgcg tcgcacttt gaactgacc 
      gaaaccaga gacacttta gccaaacta cgactgaac 
      ctatccatg gctgagaac ctaaccgaa gtccacaac 
      ctctactcg agtgtctcc gtcgagaaa caaagacta 
      cagttacgg taccaagaa atgtacctc caatgccaa 
      ctcgatgac ttgatccgg aactcgacc gtgatcttc 
      gatttccaa ctactcaag caggctttg agcaacatc 
      cacaacggc gagatggca ttcccgggg aactcccga 
      atcctcccg ccttcgcag caaaacccg atacttttc 
      cttgcaaac tattgcatg tcccaactt tactcgcgt 
      cacgtttta accaaacaa accgtcaac ggaagtctc 
      gattaccac aaccataat accatcccc cctgttaac 
      taccacccc caacattta gtcctaacc acgccgaaa 
      aacatctac caggagaac aatctaatg atgcaaagc 
      gttatacac tcgccacaa atgcgcaac ccagccaat 
      atgtgcccc ctttactac ttcaacatc gaggtgaat 
      ggggcgtcc gttggccgg attgttatc gaagtgcga 
      gcggatgtg gcaccgaaa atggccaaa aatttcggc 
      ctattgacg acaggcgag gccggaatg gggtacaaa 
      ggatgccag attttccaa tgttgggag ggggagagt 
      gtgataacg ggggatttt gagctgaat aatggacga 
      ggggggcgt tcgattttc gaagactct tattttttg 
      ccagatgat accaagttg atggccgtg agggggacg 
      gtgggcatg aggcggaca cagaaacga cacgacaat 
      ttaggaatg gtggggtcg cagttccgg atcatattg 
      caggagatg cgggggttc actggtatt ttcgggcac 
      gtgatcgaa ggcctcgaa ttggtggaa aagatcagt 
      acgtatggg gacacggcc ggaaaaccg accaaaaac 
      atacttatc gcaaaatgc ggcaaatta taa
    mRNA sequence
    • >TC006218 RA
      cggttagtc gtcaacatg atgtggcac atcgttcgg 
      cggccacaa taactttaa gttttttcg taaatctgc 
      cgatcttgt cggcacgaa acaattatg taatataaa 
      tctcgatta gttaatgca tcgtacacc gaacccgtt 
      ttaaacgca aaatagtac aaacacgcg aaaaccgtt 
      gccgaaaca ccgagtttt tataaacga cgacaattc 
      agacacaca cacacacac aacaaacga cgaccaaaa 
      acaagcgta tccctcgtt ttatcggat ttactcaaa 
      ggctgtgct acacatcgt aaacaaaaa acataaaaa 
      tttaataaa aaacgaaac gaagaatgg aaccggtgc 
      ccgaattcg aggagctga tgctgtgcg gctactgca 
      agcagaaat tcaacgact ccgaccaga gccccaaac 
      tgctatcgt gcaaacact acttctgcc tccaatgca 
      tgcgcacca acctgaaca agggccagg agctgtact 
      gcgtgtact gctggaagc gcaccgaaa tcggcgacc 
      tggggcccg agtcgctcc cgacctacg cccccgtgc 
      tctgcctca cgaagaact tctcgcaga tgaagctgg 
      gccccaagc ccccggaca aacccgcca aagtcatat 
      cggagaatt gccacacgc acgccatgc ccctggcgc 
      tctggtgcc acacgtgct gcgcccccg tgtgccgcg 
      cctgcgcca ccaccagcg accattcca cccaccaaa 
      tcaaatcgc agaacgaag ccaaggagc agctcgtcg 
      ccgagctgc aggtcgacc tcgcgtcga tgaataaaa 
      tgctctcgg agatccaga ggcttgcga tacaacagc 
      gcgaattct tgctgaaaa tactcgaag cctgcgtta 
      cgctaaaga cgcacgtcg aggccgatt tgaccaaca 
      atgcgtcgc actttgaac tgaccgaaa ccagagaca 
      ctttagcca aactacgac tgaacctat ccatggctg 
      agaacctaa ccgaagtcc acaacctct actcgagtg 
      tctccgtcg agaaacaaa gactacagt tacggtacc 
      aagaaatgt acctccaat gccaactcg atgacttga 
      tccggaact cgaccgtga tcttcgatt tccaactac 
      tcaagcagg ctttgagca acatccaca acggcgaga 
      tggcattcc cggggaact cccgaatcc tcccgcctt 
      cgcagcaaa acccgatac ttttccttg caaactatt 
      gcatgtccc aactttact cgcgtcacg ttttaacca 
      aacaaaccg tcaacggaa gtctcgatt accacaacc 
      ataatacca tcccccctg ttaactacc acccccaac 
      atttagtcc taaccacgc cgaaaaaca tctaccagg 
      agaacaatc taatgatgc aaagcgtta tacactcgc 
      cacaaatgc gcaacccag ccaatatgt gcccccttt 
      actacttca acatcgagg tgaatgggg cgtccgttg 
      gccggattg ttatcgaag tgcgagcgg atgtggcac 
      cgaaaatgg ccaaaaatt tcggcctat tgacgacag 
      gcgaggccg gaatggggt acaaaggat gccagattt 
      tccaatgtt gggaggggg agagtgtga taacggggg 
      attttgagc tgaataatg gacgagggg ggcgttcga 
      ttttcgaag actcttatt ttttgccag atgatacca 
      agttgatgg ccgtgaggg ggacggtgg gcatgaggc 
      ggacacaga aacgacacg acaatttag gaatggtgg 
      ggtcgcagt tccggatca tattgcagg agatgcggg 
      ggttcactg gtattttcg ggcacgtga tcgaaggcc 
      tcgaattgg tggaaaaga tcagtacgt atggggaca 
      cggccggaa aaccgacca aaaacatac ttatcgcaa 
      aatgcggca aattataat cggcttgaa tcgaaacac 
      ttgatgtct aatcaaact ttgcttaaa gaaaaaaac 
      acaaaaaaa tcatatatc tagatgttt tatatgtag 
      cttcaagca acagtgcgt agttgatca cgagatgaa 
      aacgtaatg ataccgtct tagcttaat agtactatc 
      gaattccga aataactat attttcgta tcgtatcaa 
      atatcgtat ataaaaaat gaaaagtac gcgatattt 
      tttattgtt gtcgttttt cagcaaggg acgttttta 
      gcttaataa gtgtctatg ttatgtttg tttcctggt 
      gcggtagaa atgttaaag attacagag tttaaaaaa 
      aatcccgga acgtcagga attgcactt ggctttttt 
      tggtacctt atagactta gtttcgaac cgaaatctg 
      gtttgggtt catcggacg tgaagaaat ctccctgtt 
      ttttagtgt ttttcatta tattacatg aatataact 
      agattaagt acggtaatt cggtaaatt gttaatttg 
      gcaatttgt acgttcaaa ccgatcagc tcgatcgac 
      tgtggtcgg tttcaacgc acaagctcc caatttggt 
      cctagtttt aagttaatt aattagtcg caaccgggt 
      atatttatc gaagattaa tataacatg ctagatcgc 
      tgtcaatgt tatgatata aaaatcatt tgaaaaaag 
      aggcaaata aaatctata tttttaacg ttctcttcg 
      ttcattctt ttaactgtc tgtcgtaac taactattt 
      gggatacaa acgaatata caacgaatt taaatcaca 
      aggggattg tccgagtgc atatcaatt tgatttttt 
      ctagtgtag agtggtatc gtctcattt tgccggaat 
      ttttttgtg ccttaatat tgttttttt tttggtcat 
      ccaatagtt tagtttagg tcttcccaa tgtatcctg 
      cctacgtcg gaactcctg gatgccaca acttcaaaa 
      ataatcaaa attaccgtt tcgcaaatc agtggcgta 
      acgattgtt tttgtcgca gacaccaat aaatgttga 
      taatacaca tcgagatta atttgggtc aacactgcc 
      ataaaatct aaacttggc aagccgtcg aggtgcaaa 
      tagtccaga aggctgttt ccatctgct acctgtcaa 
      tccaaacaa aacaacgaa atctattcg ttgacaaat 
      aaatatcac aaagattg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY