General information | |||
TC006218 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 5197018..5203045 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC006218 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04090 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment potentially partiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC006218 RA
MEPVPEFEE LMLCGYCKQ KFNDSDQSP KLLSCKHYF CLQCMRTNL NKGQELYCV YCWKRTEIG DLGPESLPT YAPVLCLTK NFSQMKLGP KPPDKPAKV ISENCHTHA MPLALWCHT CCAPVCRAC ATTSDHSTH QIKSQNEAK EQLVAELQV DLASMNKML SEIQRLAIQ QREFLLKIL EACVTLKTH VEADLTNNA SHFELTETR DTLAKLRLN LSMAENLTE VHNLYSSVS VEKQRLQLR YQEMYLQCQ LDDLIRNST VIFDFQLLK QALSNIHNG EMAFPGNSR ILPPSQQNP ILFLANYCM SQLYSRHVL TKQTVNGSL DYHNHNTIP PVNYHPQHL VLTTPKNIY QENNLMMQS VIHSPQMRN PANMCPLYY FNIEVNGAS VGRIVIEVR ADVAPKMAK NFGLLTTGE AGMGYKGCQ IFQCWEGES VITGDFELN NGRGGRSIF EDSYFLPDD TKLMAVRGT VGMRRTQKR HDNLGMVGS QFRIILQEM RGFTGIFGH VIEGLELVE KISTYGDTA GKPTKNILI AKCGKL
- >TC006218 RA
atggaaccg gtgcccgaa ttcgaggag ctgatgctg tgcggctac tgcaagcag aaattcaac gactccgac cagagcccc aaactgcta tcgtgcaaa cactacttc tgcctccaa tgcatgcgc accaacctg aacaagggc caggagctg tactgcgtg tactgctgg aagcgcacc gaaatcggc gacctgggg cccgagtcg ctcccgacc tacgccccc gtgctctgc ctcacgaag aacttctcg cagatgaag ctgggcccc aagcccccg gacaaaccc gccaaagtc atatcggag aattgccac acgcacgcc atgcccctg gcgctctgg tgccacacg tgctgcgcc cccgtgtgc cgcgcctgc gccaccacc agcgaccat tccacccac caaatcaaa tcgcagaac gaagccaag gagcagctc gtcgccgag ctgcaggtc gacctcgcg tcgatgaat aaaatgctc tcggagatc cagaggctt gcgatacaa cagcgcgaa ttcttgctg aaaatactc gaagcctgc gttacgcta aagacgcac gtcgaggcc gatttgacc aacaatgcg tcgcacttt gaactgacc gaaaccaga gacacttta gccaaacta cgactgaac ctatccatg gctgagaac ctaaccgaa gtccacaac ctctactcg agtgtctcc gtcgagaaa caaagacta cagttacgg taccaagaa atgtacctc caatgccaa ctcgatgac ttgatccgg aactcgacc gtgatcttc gatttccaa ctactcaag caggctttg agcaacatc cacaacggc gagatggca ttcccgggg aactcccga atcctcccg ccttcgcag caaaacccg atacttttc cttgcaaac tattgcatg tcccaactt tactcgcgt cacgtttta accaaacaa accgtcaac ggaagtctc gattaccac aaccataat accatcccc cctgttaac taccacccc caacattta gtcctaacc acgccgaaa aacatctac caggagaac aatctaatg atgcaaagc gttatacac tcgccacaa atgcgcaac ccagccaat atgtgcccc ctttactac ttcaacatc gaggtgaat ggggcgtcc gttggccgg attgttatc gaagtgcga gcggatgtg gcaccgaaa atggccaaa aatttcggc ctattgacg acaggcgag gccggaatg gggtacaaa ggatgccag attttccaa tgttgggag ggggagagt gtgataacg ggggatttt gagctgaat aatggacga ggggggcgt tcgattttc gaagactct tattttttg ccagatgat accaagttg atggccgtg agggggacg gtgggcatg aggcggaca cagaaacga cacgacaat ttaggaatg gtggggtcg cagttccgg atcatattg caggagatg cgggggttc actggtatt ttcgggcac gtgatcgaa ggcctcgaa ttggtggaa aagatcagt acgtatggg gacacggcc ggaaaaccg accaaaaac atacttatc gcaaaatgc ggcaaatta taa
- >TC006218 RA
cggttagtc gtcaacatg atgtggcac atcgttcgg cggccacaa taactttaa gttttttcg taaatctgc cgatcttgt cggcacgaa acaattatg taatataaa tctcgatta gttaatgca tcgtacacc gaacccgtt ttaaacgca aaatagtac aaacacgcg aaaaccgtt gccgaaaca ccgagtttt tataaacga cgacaattc agacacaca cacacacac aacaaacga cgaccaaaa acaagcgta tccctcgtt ttatcggat ttactcaaa ggctgtgct acacatcgt aaacaaaaa acataaaaa tttaataaa aaacgaaac gaagaatgg aaccggtgc ccgaattcg aggagctga tgctgtgcg gctactgca agcagaaat tcaacgact ccgaccaga gccccaaac tgctatcgt gcaaacact acttctgcc tccaatgca tgcgcacca acctgaaca agggccagg agctgtact gcgtgtact gctggaagc gcaccgaaa tcggcgacc tggggcccg agtcgctcc cgacctacg cccccgtgc tctgcctca cgaagaact tctcgcaga tgaagctgg gccccaagc ccccggaca aacccgcca aagtcatat cggagaatt gccacacgc acgccatgc ccctggcgc tctggtgcc acacgtgct gcgcccccg tgtgccgcg cctgcgcca ccaccagcg accattcca cccaccaaa tcaaatcgc agaacgaag ccaaggagc agctcgtcg ccgagctgc aggtcgacc tcgcgtcga tgaataaaa tgctctcgg agatccaga ggcttgcga tacaacagc gcgaattct tgctgaaaa tactcgaag cctgcgtta cgctaaaga cgcacgtcg aggccgatt tgaccaaca atgcgtcgc actttgaac tgaccgaaa ccagagaca ctttagcca aactacgac tgaacctat ccatggctg agaacctaa ccgaagtcc acaacctct actcgagtg tctccgtcg agaaacaaa gactacagt tacggtacc aagaaatgt acctccaat gccaactcg atgacttga tccggaact cgaccgtga tcttcgatt tccaactac tcaagcagg ctttgagca acatccaca acggcgaga tggcattcc cggggaact cccgaatcc tcccgcctt cgcagcaaa acccgatac ttttccttg caaactatt gcatgtccc aactttact cgcgtcacg ttttaacca aacaaaccg tcaacggaa gtctcgatt accacaacc ataatacca tcccccctg ttaactacc acccccaac atttagtcc taaccacgc cgaaaaaca tctaccagg agaacaatc taatgatgc aaagcgtta tacactcgc cacaaatgc gcaacccag ccaatatgt gcccccttt actacttca acatcgagg tgaatgggg cgtccgttg gccggattg ttatcgaag tgcgagcgg atgtggcac cgaaaatgg ccaaaaatt tcggcctat tgacgacag gcgaggccg gaatggggt acaaaggat gccagattt tccaatgtt gggaggggg agagtgtga taacggggg attttgagc tgaataatg gacgagggg ggcgttcga ttttcgaag actcttatt ttttgccag atgatacca agttgatgg ccgtgaggg ggacggtgg gcatgaggc ggacacaga aacgacacg acaatttag gaatggtgg ggtcgcagt tccggatca tattgcagg agatgcggg ggttcactg gtattttcg ggcacgtga tcgaaggcc tcgaattgg tggaaaaga tcagtacgt atggggaca cggccggaa aaccgacca aaaacatac ttatcgcaa aatgcggca aattataat cggcttgaa tcgaaacac ttgatgtct aatcaaact ttgcttaaa gaaaaaaac acaaaaaaa tcatatatc tagatgttt tatatgtag cttcaagca acagtgcgt agttgatca cgagatgaa aacgtaatg ataccgtct tagcttaat agtactatc gaattccga aataactat attttcgta tcgtatcaa atatcgtat ataaaaaat gaaaagtac gcgatattt tttattgtt gtcgttttt cagcaaggg acgttttta gcttaataa gtgtctatg ttatgtttg tttcctggt gcggtagaa atgttaaag attacagag tttaaaaaa aatcccgga acgtcagga attgcactt ggctttttt tggtacctt atagactta gtttcgaac cgaaatctg gtttgggtt catcggacg tgaagaaat ctccctgtt ttttagtgt ttttcatta tattacatg aatataact agattaagt acggtaatt cggtaaatt gttaatttg gcaatttgt acgttcaaa ccgatcagc tcgatcgac tgtggtcgg tttcaacgc acaagctcc caatttggt cctagtttt aagttaatt aattagtcg caaccgggt atatttatc gaagattaa tataacatg ctagatcgc tgtcaatgt tatgatata aaaatcatt tgaaaaaag aggcaaata aaatctata tttttaacg ttctcttcg ttcattctt ttaactgtc tgtcgtaac taactattt gggatacaa acgaatata caacgaatt taaatcaca aggggattg tccgagtgc atatcaatt tgatttttt ctagtgtag agtggtatc gtctcattt tgccggaat ttttttgtg ccttaatat tgttttttt tttggtcat ccaatagtt tagtttagg tcttcccaa tgtatcctg cctacgtcg gaactcctg gatgccaca acttcaaaa ataatcaaa attaccgtt tcgcaaatc agtggcgta acgattgtt tttgtcgca gacaccaat aaatgttga taatacaca tcgagatta atttgggtc aacactgcc ataaaatct aaacttggc aagccgtcg aggtgcaaa tagtccaga aggctgttt ccatctgct acctgtcaa tccaaacaa aacaacgaa atctattcg ttgacaaat aaatatcac aaagattg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
